Lag0012324 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012324
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionmitoferrin-like
Locationchr1: 40016443 .. 40018173 (-)
RNA-Seq ExpressionLag0012324
SyntenyLag0012324
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACAAGCGTCTCCCACTCTCCCGATTTCCGCCCCGAAATCTCAGTTTCGCCACCGACCCACGACGGCCTTTTCTTCTGGCAGTTCATGATCGCCGGATCAATAGCAGGGTCCGTCGAGCACATGGCCATGTTTCCAGTGGACACTCTCAAGACCCGAATACAAGCCCTCGGCGGATCATCTACAGTCCGACAAGCACTCGGGTCGATTCTGAAAGTGGAAGGTCCGGCCGGACTCTACCGTGGAATCGGAGCAATGGGTCTGGGCGCAGGGCCTGCACACGCTGTATACTTCTCGGTATACGAATTCTGCAAAGAGGGGTTTTCGATTGGAAATCCGAACAACCCATTAGGGCACGCCATTTCTGGAGTGTGTGCGACGGTGGCGAGCGACGCCGTGATAACGCCGATGGATGTGGTGAAACAGAGGCTGCAGTTGGAGAGCAGTCCTTATAAGGGAGTGGGGGACTGTGTGAGGAGGGTTTTGGTTGAGGAAGGAATTGGGGCGCTCTATGCTTCCTATCGGACCACGGTTTTGATGAATGCGCCGTACACGGCTGTCCATTTTGCCACTTATGAAGCTGCGAAAAGGGGCTTGAAGGAAGTCTCGCCGGGAAGTGACGACGATGAGGGGTTGGTTGTTCATGCTACGGCCGGTGCGGCAGCTGGGTCTATTGCTGCTGCTCTTACAACGCCATTAGACGTTGTGAAAACTCGGCTGCAATGCCAGGTAATGACTGTTTGTGTTTCTTGCCGGCCTGGTAACTTCGCATGATCATAGAACTTTTTTTTTTTTTTTTTTTTAATTTGCGAGTGTAAAGTCATATAGGTTCTGTTACAAATTTAGCTAGAGCTTTGGTTCCTGGACTTTAAAATTTTATATTCAAGTATTCATCTTTGAATTTGAATGTGACCTAAGCCCTTATCATGTCAAGTTGACTAAGTGGGAAGATTTGGCATAATTAGACAAAAGAGAATAAACAAATGAACAAAATCAAGGGACATTTACAACATATACCATGTTCTGTCATTTGTGCGATCAACCGTGCTAATATTAGAGAATTATATATTAATTCAAATTTAAACTTCTACTATTGAAACTTTTGAAAGTTCTCTTTTCATGAAAGCCTTTAGTTTTTGAAAAATAAAAAATATAGTTTAAAAAAACGTAATATTGTATTTGGAATTTGACTAGGAATTCAAACATGTCATTTATAAAGATACAAAGATGAAGAAAAAACCAGCATAGTTTTAGAACCAAGTTATCCAATGAGACCTAAATTAGTGATTTAACAAGTAAAAATGGAGATCCCACAGATGAGGATGAGGAATTTTGTCGAACAAATGGAGGGGAATAATTCTTCACTCCGTTCCAGTTTTCCAGGAAAAAGAGCATAACCAGATTGCATCAACACCCACCAAGTTAACACTCGTTTAACTTAAATAGAAACTATTCTATTCTCTAATTTACAAACAGCTATAACAGGAAATTTCATGTGTTTCTCTAATGGTTTTACTGTACAAACAGGGAGTGTGTGGGTGCGACAGATTTTCAAGCAGTTCGATAGGATACGTACTTGGGCATGTGGTGAAGAAGGATGGGTACAATGGCCTGATGAGAGGATGGCTCCCAAGGATGCTGTTCCATGCGCCTGCTGCTGCAATTTGCTGGTCCACTTACGAAGCTTCAAAAACCTTCTTTCAACAGCTCCACCACGACAACAATTAG

mRNA sequence

ATGGCCACAAGCGTCTCCCACTCTCCCGATTTCCGCCCCGAAATCTCAGTTTCGCCACCGACCCACGACGGCCTTTTCTTCTGGCAGTTCATGATCGCCGGATCAATAGCAGGGTCCGTCGAGCACATGGCCATGTTTCCAGTGGACACTCTCAAGACCCGAATACAAGCCCTCGGCGGATCATCTACAGTCCGACAAGCACTCGGGTCGATTCTGAAAGTGGAAGGTCCGGCCGGACTCTACCGTGGAATCGGAGCAATGGGTCTGGGCGCAGGGCCTGCACACGCTGTATACTTCTCGGTATACGAATTCTGCAAAGAGGGGTTTTCGATTGGAAATCCGAACAACCCATTAGGGCACGCCATTTCTGGAGTGTGTGCGACGGTGGCGAGCGACGCCGTGATAACGCCGATGGATGTGGTGAAACAGAGGCTGCAGTTGGAGAGCAGTCCTTATAAGGGAGTGGGGGACTGTGTGAGGAGGGTTTTGGTTGAGGAAGGAATTGGGGCGCTCTATGCTTCCTATCGGACCACGGTTTTGATGAATGCGCCGTACACGGCTGTCCATTTTGCCACTTATGAAGCTGCGAAAAGGGGCTTGAAGGAAGTCTCGCCGGGAAGTGACGACGATGAGGGGTTGGTTGTTCATGCTACGGCCGGTGCGGCAGCTGGGTCTATTGCTGCTGCTCTTACAACGCCATTAGACGTTGTGAAAACTCGGCTGCAATGCCAGGGAGTGTGTGGGTGCGACAGATTTTCAAGCAGTTCGATAGGATACGTACTTGGGCATGTGGTGAAGAAGGATGGGTACAATGGCCTGATGAGAGGATGGCTCCCAAGGATGCTGTTCCATGCGCCTGCTGCTGCAATTTGCTGGTCCACTTACGAAGCTTCAAAAACCTTCTTTCAACAGCTCCACCACGACAACAATTAG

Coding sequence (CDS)

ATGGCCACAAGCGTCTCCCACTCTCCCGATTTCCGCCCCGAAATCTCAGTTTCGCCACCGACCCACGACGGCCTTTTCTTCTGGCAGTTCATGATCGCCGGATCAATAGCAGGGTCCGTCGAGCACATGGCCATGTTTCCAGTGGACACTCTCAAGACCCGAATACAAGCCCTCGGCGGATCATCTACAGTCCGACAAGCACTCGGGTCGATTCTGAAAGTGGAAGGTCCGGCCGGACTCTACCGTGGAATCGGAGCAATGGGTCTGGGCGCAGGGCCTGCACACGCTGTATACTTCTCGGTATACGAATTCTGCAAAGAGGGGTTTTCGATTGGAAATCCGAACAACCCATTAGGGCACGCCATTTCTGGAGTGTGTGCGACGGTGGCGAGCGACGCCGTGATAACGCCGATGGATGTGGTGAAACAGAGGCTGCAGTTGGAGAGCAGTCCTTATAAGGGAGTGGGGGACTGTGTGAGGAGGGTTTTGGTTGAGGAAGGAATTGGGGCGCTCTATGCTTCCTATCGGACCACGGTTTTGATGAATGCGCCGTACACGGCTGTCCATTTTGCCACTTATGAAGCTGCGAAAAGGGGCTTGAAGGAAGTCTCGCCGGGAAGTGACGACGATGAGGGGTTGGTTGTTCATGCTACGGCCGGTGCGGCAGCTGGGTCTATTGCTGCTGCTCTTACAACGCCATTAGACGTTGTGAAAACTCGGCTGCAATGCCAGGGAGTGTGTGGGTGCGACAGATTTTCAAGCAGTTCGATAGGATACGTACTTGGGCATGTGGTGAAGAAGGATGGGTACAATGGCCTGATGAGAGGATGGCTCCCAAGGATGCTGTTCCATGCGCCTGCTGCTGCAATTTGCTGGTCCACTTACGAAGCTTCAAAAACCTTCTTTCAACAGCTCCACCACGACAACAATTAG

Protein sequence

MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGGSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLGHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASKTFFQQLHHDNN
Homology
BLAST of Lag0012324 vs. NCBI nr
Match: XP_022144050.1 (mitoferrin-like [Momordica charantia])

HSP 1 Score: 588.6 bits (1516), Expect = 3.2e-164
Identity = 286/315 (90.79%), Postives = 301/315 (95.56%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGG 60
           MATSVSHSPDF PEISVSP THDGL+FWQFM+AGSIAGSVEHMAMFPVDTLKTR+QALGG
Sbjct: 1   MATSVSHSPDFHPEISVSPQTHDGLYFWQFMVAGSIAGSVEHMAMFPVDTLKTRMQALGG 60

Query: 61  SST-----VRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPN 120
           SS      VRQAL SILK+EGPAGLYRGIGAMGLGAGPAHAVYF+VYE CKEGF++GNPN
Sbjct: 61  SSPIQPIGVRQALSSILKLEGPAGLYRGIGAMGLGAGPAHAVYFTVYEICKEGFTVGNPN 120

Query: 121 NPLGHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASY 180
           NP+GHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRR+LVEEGIGALYASY
Sbjct: 121 NPIGHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRILVEEGIGALYASY 180

Query: 181 RTTVLMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLD 240
           RTTVLMNAPYTAVHFATYE AKRGLKE+SPGSD+DE LVVHATAGAAAGS+AAALTTPLD
Sbjct: 181 RTTVLMNAPYTAVHFATYEVAKRGLKEISPGSDNDEKLVVHATAGAAAGSLAAALTTPLD 240

Query: 241 VVKTRLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTY 300
            VKT+LQCQGVCGCDRFSSSSIGYVLG VVKKDGYNGLMRGW+PRMLFHAPAAAICWSTY
Sbjct: 241 AVKTQLQCQGVCGCDRFSSSSIGYVLGRVVKKDGYNGLMRGWIPRMLFHAPAAAICWSTY 300

Query: 301 EASKTFFQQLHHDNN 311
           EASKTFFQQLHHDN+
Sbjct: 301 EASKTFFQQLHHDNS 315

BLAST of Lag0012324 vs. NCBI nr
Match: XP_038881976.1 (mitoferrin-like [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 6.0e-163
Identity = 283/311 (91.00%), Postives = 303/311 (97.43%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPD+RPE+SV PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDYRPEVSVPPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSMGNDNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAVITPMDVVKQRLQL+SSPYKGVGDCVRR+LVEEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLKSSPYKGVGDCVRRILVEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEV+PGSD+DE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVAPGSDNDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ LH+DNN
Sbjct: 301 SFFQHLHNDNN 311

BLAST of Lag0012324 vs. NCBI nr
Match: XP_008464226.1 (PREDICTED: mitoferrin-like [Cucumis melo] >KAA0037726.1 mitoferrin-like [Cucumis melo var. makuwa])

HSP 1 Score: 582.0 bits (1499), Expect = 3.0e-162
Identity = 283/311 (91.00%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAVITPMDVVKQRLQLE+SPYKGVGDCVRR+L EEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLENSPYKGVGDCVRRILAEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSDDDE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDDDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ LH+ NN
Sbjct: 301 SFFQHLHNHNN 311

BLAST of Lag0012324 vs. NCBI nr
Match: TYK10935.1 (mitoferrin-like [Cucumis melo var. makuwa])

HSP 1 Score: 577.4 bits (1487), Expect = 7.4e-161
Identity = 281/311 (90.35%), Postives = 300/311 (96.46%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAVITPMDVVKQRLQL++SPYKGVGDCVRR+L EEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLKNSPYKGVGDCVRRILAEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSDDDE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDDDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ L + NN
Sbjct: 301 SFFQHLQNHNN 311

BLAST of Lag0012324 vs. NCBI nr
Match: XP_004148606.1 (mitoferrin [Cucumis sativus] >KGN54404.1 hypothetical protein Csa_018144 [Cucumis sativus])

HSP 1 Score: 576.6 bits (1485), Expect = 1.3e-160
Identity = 280/311 (90.03%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAV+TPMDVVKQRLQL+SSPYKGV DCV+R+LVEEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVLTPMDVVKQRLQLKSSPYKGVRDCVKRILVEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSD+DE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDEDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ LH+ NN
Sbjct: 301 SFFQHLHNHNN 311

BLAST of Lag0012324 vs. ExPASy Swiss-Prot
Match: Q55DY8 (Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-55
Identity = 126/297 (42.42%), Postives = 181/297 (60.94%), Query Frame = 0

Query: 23  DGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL--GGSSTVR-QALGSILKVEGPAG 82
           +G  F+  +IAG+ AG  EH  M+P+DT+KT IQA+  G   T   Q    I++  G  G
Sbjct: 12  EGGSFYVHLIAGAAAGFAEHCGMYPIDTIKTHIQAIKPGAMQTSSLQITKHIIQQHGITG 71

Query: 83  LYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGN--PNNPLGHAISGVCATVASDAVITP 142
           L+RG+ A+  GA P+HAV+FS+YE  K  F IG+   ++P+   I+G  AT+ S+AV +P
Sbjct: 72  LFRGLTAVAAGAAPSHAVHFSIYELLKFKF-IGSDEDHHPIKVGIAGAIATMTSEAVASP 131

Query: 143 MDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAK 202
           MDVVKQRLQL+ + YKG+ DC +R+ V+EGI   Y+ Y TT++MN PY  V+FA+YE+ K
Sbjct: 132 MDVVKQRLQLQITDYKGLTDCTKRIWVKEGIRGFYSGYTTTLVMNVPYNIVYFASYESLK 191

Query: 203 RGLKEVSPGSDDDE---GLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQGVCGCDRFSS 262
           + ++      + +E    L+ H  AG  AG +AAA T P DVVKTRLQ Q     D  +S
Sbjct: 192 KIIQPWFNNKNPEERSYQLIDHLVAGGGAGMLAAAFTNPFDVVKTRLQTQS----DFIAS 251

Query: 263 SSIGY------------VLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           S+I               +  +  ++G +G +RG  PRM+FH+ ++AI WS YE  K
Sbjct: 252 STINSAKSIKRYGGMMDAMKTIWIEEGMDGYLRGMKPRMVFHSMSSAIVWSVYEYFK 303

BLAST of Lag0012324 vs. ExPASy Swiss-Prot
Match: P10566 (Mitochondrial RNA-splicing protein MRS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MRS3 PE=1 SV=4)

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-51
Identity = 117/297 (39.39%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 20  PTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGGSS-TVRQALGSILKV---E 79
           PTH  L+    +IAG+ AG +EH  MFP+D LKTRIQ+    S + +  L  I  +   E
Sbjct: 28  PTHAPLY--HQLIAGAFAGIMEHSVMFPIDALKTRIQSANAKSLSAKNMLSQISHISTSE 87

Query: 80  GPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGF---SIGNPNNPLGHAISGVCATVASD 139
           G   L++G+ ++ LGAGPAHAVYF  YEFCK+     S    ++P   AISG CAT ASD
Sbjct: 88  GTLALWKGVQSVILGAGPAHAVYFGTYEFCKKNLIDSSDTQTHHPFKTAISGACATTASD 147

Query: 140 AVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFAT 199
           A++ P D +KQR+QL +S    V    +++   EG+ A Y SY TT++MN P+ A +F  
Sbjct: 148 ALMNPFDTIKQRIQLNTS--ASVWQTTKQIYQSEGLAAFYYSYPTTLVMNIPFAAFNFVI 207

Query: 200 YEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQG------- 259
           YE++ + L        ++   ++H   G+ +GS  AA+TTPLD +KT LQ +G       
Sbjct: 208 YESSTKFL-----NPSNEYNPLIHCLCGSISGSTCAAITTPLDCIKTVLQIRGSQTVSLE 267

Query: 260 -VCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASKTF 302
            +   D FS ++       + +  G+ G  RGW PR++ + PA AI W+ YE +K F
Sbjct: 268 IMRKADTFSKAA-----SAIYQVYGWKGFWRGWKPRIVANMPATAISWTAYECAKHF 310

BLAST of Lag0012324 vs. ExPASy Swiss-Prot
Match: O14281 (Uncharacterized mitochondrial carrier C8C9.12c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC8C9.12c PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-49
Identity = 112/276 (40.58%), Postives = 161/276 (58.33%), Query Frame = 0

Query: 31  MIAGSIAGSVEHMAMFPVDTLKTRIQALGG--SSTVRQALGSILKV---EGPAGLYRGIG 90
           ++AG+ +G +EH  M+PVD +KTR+Q L G   S     + S++K+   EG   L+RGI 
Sbjct: 23  LLAGAFSGILEHSVMYPVDAIKTRMQMLNGVSRSVSGNIVNSVIKISSTEGVYSLWRGIS 82

Query: 91  AMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLGHAISGVCATVASDAVITPMDVVKQRL 150
           ++ +GAGP+HA+YFSV EF K   +  +P+ PL  A++G CA   SDA +TP DV+KQR+
Sbjct: 83  SVIMGAGPSHAIYFSVLEFFKSKIN-ASPDRPLASALAGACAITISDAFMTPFDVIKQRM 142

Query: 151 QLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAKRGLKEVSP 210
           QL S  YK    C   V   EG+GA Y SY T + M+ P+TA+  ATY+     +  ++P
Sbjct: 143 QLPSRKYKSALHCATTVFRNEGLGAFYISYPTCIAMSIPFTAIQVATYDTC---MSFLNP 202

Query: 211 GSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQGVCGCD--RFSSSSIGYVLGH 270
            +  D     H  +G  +G+IA++LTTPLDVVKT LQ +G       R    S+  V+  
Sbjct: 203 NAVYDP--TSHIISGGLSGAIASSLTTPLDVVKTLLQTRGSSSIPEVRKCKGSLD-VVRF 262

Query: 271 VVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           +    G     +G  PRM+   PA A+ W+ YEA K
Sbjct: 263 IYNYGGIPSFFKGIRPRMVVAMPATAVSWAAYEAGK 291

BLAST of Lag0012324 vs. ExPASy Swiss-Prot
Match: Q9VAY3 (Mitoferrin OS=Drosophila melanogaster OX=7227 GN=mfrn PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.9e-48
Identity = 116/286 (40.56%), Postives = 164/286 (57.34%), Query Frame = 0

Query: 31  MIAGSIAGSVEHMAMFPVDTLKTRIQALG---GSSTVRQALGSILKVEGPAGLYRGIGAM 90
           M AG+IAG +EH+ M+P+D++KTR+Q+L     +  +   L +++  EG     RG  A+
Sbjct: 18  MTAGAIAGVLEHVVMYPLDSVKTRMQSLSPPTKNMNIVSTLRTMITREGLLRPIRGASAV 77

Query: 91  GLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLGHAISGVCATVASDAVITPMDVVKQRLQL 150
            LGAGPAH++YF+ YE  KE  +       L + ISG  AT+  DA+ +P DV+KQR+Q+
Sbjct: 78  VLGAGPAHSLYFAAYEMTKELTAKFTSVRNLNYVISGAVATLIHDAISSPTDVIKQRMQM 137

Query: 151 ESSPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAKRGL---KEVS 210
            +SPY  V  CVR +   EG  A Y +Y T ++MN PY  +HF TYE  +  +   ++ +
Sbjct: 138 YNSPYTSVVSCVRDIYKREGFKAFYRAYGTQLVMNLPYQTIHFTTYEFFQNKMNLERKYN 197

Query: 211 PGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQ--GVCGCDRFSSSSIGYVLG 270
           P         VH  AGAAAG+ AAA+TTPLDV+KT L  Q  G+      +S  I ++ G
Sbjct: 198 P--------PVHMAAGAAAGACAAAVTTPLDVIKTLLNTQETGLTRGMIEASRKIYHMAG 257

Query: 271 HVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASKTFFQQLHHD 309
            +       G  RG   R+L+  PA AICWSTYE  K +   L  D
Sbjct: 258 PL-------GFFRGTTARVLYSMPATAICWSTYEFFKFYLCGLDAD 288

BLAST of Lag0012324 vs. ExPASy Swiss-Prot
Match: Q96A46 (Mitoferrin-2 OS=Homo sapiens OX=9606 GN=SLC25A28 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.4e-47
Identity = 118/312 (37.82%), Postives = 164/312 (52.56%), Query Frame = 0

Query: 5   VSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGGSSTV 64
           V   PD  P+    P    G      M+AG++AG +EH  M+P+D +KTR+Q+L      
Sbjct: 53  VRQDPDSGPDYEALPA---GATVTTHMVAGAVAGILEHCVMYPIDCVKTRMQSLQPDPAA 112

Query: 65  R-----QALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFS-IGNP--NN 124
           R     +AL  I++ EG     RG+     GAGPAHA+YF+ YE  K+  S + +P  N+
Sbjct: 113 RYRNVLEALWRIIRTEGLWRPMRGLNVTATGAGPAHALYFACYEKLKKTLSDVIHPGGNS 172

Query: 125 PLGHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYR 184
            + +  +G  AT+  DA + P +VVKQR+Q+ +SPY  V DCVR V   EG GA Y SY 
Sbjct: 173 HIANGAAGCVATLLHDAAMNPAEVVKQRMQMYNSPYHRVTDCVRAVWQNEGAGAFYRSYT 232

Query: 185 TTVLMNAPYTAVHFATYEAAKRGL---KEVSPGSDDDEGLVVHATAGAAAGSIAAALTTP 244
           T + MN P+ A+HF TYE  +      +  +P S        H  +GA AG++AAA TTP
Sbjct: 233 TQLTMNVPFQAIHFMTYEFLQEHFNPQRRYNPSS--------HVLSGACAGAVAAAATTP 292

Query: 245 LDVVKTRLQCQGVCGCDRFSSSSIGYVLG------HVVKKDGYNGLMRGWLPRMLFHAPA 300
           LDV KT L  Q        +S   G++ G       V +  G     RG   R+++  P+
Sbjct: 293 LDVCKTLLNTQESLA---LNSHITGHITGMASAFRTVYQVGGVTAYFRGVQARVIYQIPS 350

BLAST of Lag0012324 vs. ExPASy TrEMBL
Match: A0A6J1CQJ6 (mitoferrin-like OS=Momordica charantia OX=3673 GN=LOC111013836 PE=3 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-164
Identity = 286/315 (90.79%), Postives = 301/315 (95.56%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGG 60
           MATSVSHSPDF PEISVSP THDGL+FWQFM+AGSIAGSVEHMAMFPVDTLKTR+QALGG
Sbjct: 1   MATSVSHSPDFHPEISVSPQTHDGLYFWQFMVAGSIAGSVEHMAMFPVDTLKTRMQALGG 60

Query: 61  SST-----VRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPN 120
           SS      VRQAL SILK+EGPAGLYRGIGAMGLGAGPAHAVYF+VYE CKEGF++GNPN
Sbjct: 61  SSPIQPIGVRQALSSILKLEGPAGLYRGIGAMGLGAGPAHAVYFTVYEICKEGFTVGNPN 120

Query: 121 NPLGHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASY 180
           NP+GHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRR+LVEEGIGALYASY
Sbjct: 121 NPIGHAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRILVEEGIGALYASY 180

Query: 181 RTTVLMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLD 240
           RTTVLMNAPYTAVHFATYE AKRGLKE+SPGSD+DE LVVHATAGAAAGS+AAALTTPLD
Sbjct: 181 RTTVLMNAPYTAVHFATYEVAKRGLKEISPGSDNDEKLVVHATAGAAAGSLAAALTTPLD 240

Query: 241 VVKTRLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTY 300
            VKT+LQCQGVCGCDRFSSSSIGYVLG VVKKDGYNGLMRGW+PRMLFHAPAAAICWSTY
Sbjct: 241 AVKTQLQCQGVCGCDRFSSSSIGYVLGRVVKKDGYNGLMRGWIPRMLFHAPAAAICWSTY 300

Query: 301 EASKTFFQQLHHDNN 311
           EASKTFFQQLHHDN+
Sbjct: 301 EASKTFFQQLHHDNS 315

BLAST of Lag0012324 vs. ExPASy TrEMBL
Match: A0A5A7T6D3 (Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00190 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 283/311 (91.00%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAVITPMDVVKQRLQLE+SPYKGVGDCVRR+L EEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLENSPYKGVGDCVRRILAEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSDDDE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDDDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ LH+ NN
Sbjct: 301 SFFQHLHNHNN 311

BLAST of Lag0012324 vs. ExPASy TrEMBL
Match: A0A1S3CL00 (mitoferrin-like OS=Cucumis melo OX=3656 GN=LOC103502157 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 283/311 (91.00%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAVITPMDVVKQRLQLE+SPYKGVGDCVRR+L EEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLENSPYKGVGDCVRRILAEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSDDDE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDDDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ LH+ NN
Sbjct: 301 SFFQHLHNHNN 311

BLAST of Lag0012324 vs. ExPASy TrEMBL
Match: A0A5D3CHC5 (Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00150 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.6e-161
Identity = 281/311 (90.35%), Postives = 300/311 (96.46%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAVITPMDVVKQRLQL++SPYKGVGDCVRR+L EEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLKNSPYKGVGDCVRRILAEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSDDDE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDDDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ L + NN
Sbjct: 301 SFFQHLQNHNN 311

BLAST of Lag0012324 vs. ExPASy TrEMBL
Match: A0A0A0L006 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G312860 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.1e-161
Identity = 280/311 (90.03%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MATSVSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-G 60
           MATSVSHSPDFRPE+SV+PPTHDGL+FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL G
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLG 120
           GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEF KEGFS+GN NNPL 
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTV 180
           HAI+GVCATV SDAV+TPMDVVKQRLQL+SSPYKGV DCV+R+LVEEGIGALYASYRTTV
Sbjct: 121 HAIAGVCATVTSDAVLTPMDVVKQRLQLKSSPYKGVRDCVKRILVEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAVHFATYEAAKRGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 240
           +MNAPYTAV+FATYEAAKRGLKEVSPGSD+DE L+VHATAGAAAGS+AAALTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDEDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 300
           RLQCQGVCGCD+FSSSSIGYVLG VVKKDGYNGLM+GW+PRM+FHAPAAAICWSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TFFQQLHHDNN 311
           +FFQ LH+ NN
Sbjct: 301 SFFQHLHNHNN 311

BLAST of Lag0012324 vs. TAIR 10
Match: AT1G07030.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 438.7 bits (1127), Expect = 3.8e-123
Identity = 221/311 (71.06%), Postives = 254/311 (81.67%), Query Frame = 0

Query: 5   VSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGGSST- 64
           VS +PDF+PEI+     HDGL FWQFMIAGSIAGSVEHMAMFPVDT+KT +QAL      
Sbjct: 19  VSQTPDFKPEIA-----HDGLKFWQFMIAGSIAGSVEHMAMFPVDTIKTHMQALRPCPLK 78

Query: 65  ---VRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLGH 124
              +R+A  SI++ EGP+ LYRGI AMGLGAGPAHAVYFS YE  K+  S G+ NN + H
Sbjct: 79  PVGIREAFRSIIQKEGPSALYRGIWAMGLGAGPAHAVYFSFYEVSKKYLSAGDQNNSVAH 138

Query: 125 AISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVL 184
           A+SGV AT++SDAV TPMD+VKQRLQ+    YKGV DCV+RVL EEGIGA YASYRTTVL
Sbjct: 139 AMSGVFATISSDAVFTPMDMVKQRLQMGEGTYKGVWDCVKRVLREEGIGAFYASYRTTVL 198

Query: 185 MNAPYTAVHFATYEAAKRGLKEVSPGS-DDDEGLVVHATAGAAAGSIAAALTTPLDVVKT 244
           MNAP+TAVHFATYEAAK+GL E SP    D+EG +VHATAGAAAG +AAA+TTPLDVVKT
Sbjct: 199 MNAPFTAVHFATYEAAKKGLMEFSPDRISDEEGWLVHATAGAAAGGLAAAVTTPLDVVKT 258

Query: 245 RLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASK 304
           +LQCQGVCGCDRF+SSSI +VL  +VKKDGY GL+RGWLPRMLFHAPAAAICWSTYE  K
Sbjct: 259 QLQCQGVCGCDRFTSSSISHVLRTIVKKDGYRGLLRGWLPRMLFHAPAAAICWSTYEGVK 318

Query: 305 TFFQQLHHDNN 311
           +FFQ  + D+N
Sbjct: 319 SFFQDFNVDSN 324

BLAST of Lag0012324 vs. TAIR 10
Match: AT2G30160.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 421.0 bits (1081), Expect = 8.2e-118
Identity = 212/314 (67.52%), Postives = 245/314 (78.03%), Query Frame = 0

Query: 5   VSHSPDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGGSST- 64
           +   PDF P I V P  +  L FWQ M+AGSIAGSVEHMAMFPVDT+KT +QAL      
Sbjct: 17  IPQPPDFHPAIIV-PAQNTTLKFWQLMVAGSIAGSVEHMAMFPVDTVKTHMQALRSCPIK 76

Query: 65  ---VRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNNPLGH 124
              +RQA  SI+K +GP+ LYRGI AMGLGAGPAHAVYFS YE  K+  S GNPNN   H
Sbjct: 77  PIGIRQAFRSIIKTDGPSALYRGIWAMGLGAGPAHAVYFSFYEVSKKFLSGGNPNNSAAH 136

Query: 125 AISGVCATVASDAVITPMDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVL 184
           AISGV AT++SDAV TPMD+VKQRLQ+ +  YKGV DC++RV  EEG GA YASYRTTVL
Sbjct: 137 AISGVFATISSDAVFTPMDMVKQRLQIGNGTYKGVWDCIKRVTREEGFGAFYASYRTTVL 196

Query: 185 MNAPYTAVHFATYEAAKRGLKEVSP----GSDDDEGLVVHATAGAAAGSIAAALTTPLDV 244
           MNAP+TAVHF TYEA KRGL+E+ P    G++D+EG +++ATAGAAAG +AAA+TTPLDV
Sbjct: 197 MNAPFTAVHFTTYEAVKRGLREMLPEHAVGAEDEEGWLIYATAGAAAGGLAAAVTTPLDV 256

Query: 245 VKTRLQCQGVCGCDRFSSSSIGYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYE 304
           VKT+LQCQGVCGCDRF SSSI  V   +VKKDGY GL RGWLPRMLFHAPAAAICWSTYE
Sbjct: 257 VKTQLQCQGVCGCDRFKSSSISDVFRTIVKKDGYRGLARGWLPRMLFHAPAAAICWSTYE 316

Query: 305 ASKTFFQQLHHDNN 311
             K+FFQ L+ + N
Sbjct: 317 TVKSFFQDLNGEAN 329

BLAST of Lag0012324 vs. TAIR 10
Match: AT1G34065.1 (S-adenosylmethionine carrier 2 )

HSP 1 Score: 139.0 bits (349), Expect = 6.3e-33
Identity = 91/287 (31.71%), Postives = 144/287 (50.17%), Query Frame = 0

Query: 20  PTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALGGSSTVRQALGSILKVEGPAG 79
           P H     ++ +I G +AG V   A++P+DT+KTRIQ       V +  G I+      G
Sbjct: 71  PCHFLRVLYESLITGGLAGVVVEAALYPIDTIKTRIQ-------VARDGGKIIW----KG 130

Query: 80  LYRGIGAMGLGAGPAHAVYFSVYEFCKEGFSIGNPNN--PLGHAISGVCATVASDAVITP 139
           LY G+G   +G  PA A++F VYE  K+      P+N   + H  +G      S  V  P
Sbjct: 131 LYSGLGGNLVGVLPASALFFGVYEPTKQKLLKVLPDNLSAVAHLAAGALGGAVSSIVRVP 190

Query: 140 MDVVKQRLQLESSPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAK 199
            +VVKQR+Q  +  +    D VR ++ +EG G +YA Y + +L + P+ A+ F  YE  +
Sbjct: 191 TEVVKQRMQ--TGQFVSAPDAVRLIIAKEGFGGMYAGYGSFLLRDLPFDALQFCVYEQLR 250

Query: 200 RGLKEVSPGSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQGVCGCDRFSSSSI 259
            G K  +    +D     +A  GA AG++   LTTPLDV+KTRL  QG           +
Sbjct: 251 IGYKLAARRDLNDP---ENAMIGAFAGAVTGVLTTPLDVIKTRLMVQG----SGTQYKGV 310

Query: 260 GYVLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASKTFFQQ 305
              +  +++++G + L +G  PR+L+     +I +   E +K    +
Sbjct: 311 SDCIKTIIREEGSSALWKGMGPRVLWIGIGGSIFFGVLEKTKQILSE 337

BLAST of Lag0012324 vs. TAIR 10
Match: AT1G74240.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.4e-32
Identity = 108/358 (30.17%), Postives = 163/358 (45.53%), Query Frame = 0

Query: 1   MATSVSHS------PDFRPEISVSPPTHDGLFFWQFMIAGSIAGSVEHMAMFPVDTLKTR 60
           MAT  S S      P FR  + +   THD  F W+  + G IAG+     M PVDTLKTR
Sbjct: 1   MATKSSDSSHKQSPPSFRKSVEIK-ATHDQFFVWREFLWGGIAGAFGEGMMHPVDTLKTR 60

Query: 61  IQ------ALGGSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKEG 120
           +Q      A     ++ Q L ++   +G  G YRGI     G+    A YF   E  K+ 
Sbjct: 61  LQSQIIMNATQRQKSILQMLRTVWVGDGLKGFYRGIAPGVTGSLATGATYFGFIESTKKW 120

Query: 121 FSIGNPN--NPLGHAISGVCATVASDAVITPMDVVKQRLQLESSP--------------- 180
               +P+      H I+G         +  P +V+KQR+Q++ +                
Sbjct: 121 IEESHPSLAGHWAHFIAGAVGDTLGSFIYVPCEVIKQRMQIQGTSSSWSSYISRNSVPVQ 180

Query: 181 --------YKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAK----RG 240
                   Y G+      +  E+G   LYA Y +T+  + P+  +    YE  K    +G
Sbjct: 181 PRGDMYGYYTGMFQAGCSIWKEQGPKGLYAGYWSTLARDVPFAGLMVVFYEGLKDLTDQG 240

Query: 241 LKEVSP--GSDDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQGVCGCDRFSSSSI 300
            K+      +   EGLV+    G  AG ++A LTTPLDVVKTRLQ QG         S+I
Sbjct: 241 KKKFPQYGVNSSIEGLVL----GGLAGGLSAYLTTPLDVVKTRLQVQG---------STI 300

Query: 301 GY-----VLGHVVKKDGYNGLMRGWLPRMLFHAPAAAICWSTYEASKTFFQQLHHDNN 311
            Y      +G + +K+G  G  RG +PR++++ PA+A+ +   E  +  F++  ++NN
Sbjct: 301 KYKGWLDAVGQIWRKEGPQGFFRGSVPRVMWYLPASALTFMAVEFLRDNFREKSNNNN 344

BLAST of Lag0012324 vs. TAIR 10
Match: AT4G39460.1 (S-adenosylmethionine carrier 1 )

HSP 1 Score: 131.7 bits (330), Expect = 1.0e-30
Identity = 90/277 (32.49%), Postives = 140/277 (50.54%), Query Frame = 0

Query: 32  IAGSIAGSVEHMAMFPVDTLKTRIQALGGSSTVRQALGSILKVEGPAGLYRGIGAMGLGA 91
           IAG  AG V   A++P+DT+KTR+QA  G   +      +LK     GLY G+     G 
Sbjct: 59  IAGGTAGVVVETALYPIDTIKTRLQAARGGGKI------VLK-----GLYSGLAGNIAGV 118

Query: 92  GPAHAVYFSVYEFCKEGFSIGNPN--NPLGHAISGVCATVASDAVITPMDVVKQRLQLES 151
            PA A++  VYE  K+      P+  + + H  +G    +A+  +  P +VVKQR+Q  +
Sbjct: 119 LPASALFVGVYEPTKQKLLKTFPDHLSAVAHLTAGAIGGLAASLIRVPTEVVKQRMQ--T 178

Query: 152 SPYKGVGDCVRRVLVEEGIGALYASYRTTVLMNAPYTAVHFATYEAAKRGLKEVSPG--S 211
             +      VR +  +EG   LYA YR+ +L + P+ A+ F  YE    G K+ +    S
Sbjct: 179 GQFTSAPSAVRMIASKEGFRGLYAGYRSFLLRDLPFDAIQFCIYEQLCLGYKKAARRELS 238

Query: 212 DDDEGLVVHATAGAAAGSIAAALTTPLDVVKTRLQCQGVCGCDRFSSSSIGYVLGHVVKK 271
           D +  L+     GA AG++  A+TTPLDV+KTRL  QG           I   +  +V++
Sbjct: 239 DPENALI-----GAFAGALTGAVTTPLDVIKTRLMVQG----SAKQYQGIVDCVQTIVRE 298

Query: 272 DGYNGLMRGWLPRMLFHAPAAAICWSTYEASKTFFQQ 305
           +G   L++G  PR+L+     +I +   E++K    Q
Sbjct: 299 EGAPALLKGIGPRVLWIGIGGSIFFGVLESTKRTLAQ 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144050.13.2e-16490.79mitoferrin-like [Momordica charantia][more]
XP_038881976.16.0e-16391.00mitoferrin-like [Benincasa hispida][more]
XP_008464226.13.0e-16291.00PREDICTED: mitoferrin-like [Cucumis melo] >KAA0037726.1 mitoferrin-like [Cucumis... [more]
TYK10935.17.4e-16190.35mitoferrin-like [Cucumis melo var. makuwa][more]
XP_004148606.11.3e-16090.03mitoferrin [Cucumis sativus] >KGN54404.1 hypothetical protein Csa_018144 [Cucumi... [more]
Match NameE-valueIdentityDescription
Q55DY81.5e-5542.42Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1[more]
P105666.5e-5139.39Mitochondrial RNA-splicing protein MRS3 OS=Saccharomyces cerevisiae (strain ATCC... [more]
O142813.6e-4940.58Uncharacterized mitochondrial carrier C8C9.12c OS=Schizosaccharomyces pombe (str... [more]
Q9VAY33.9e-4840.56Mitoferrin OS=Drosophila melanogaster OX=7227 GN=mfrn PE=2 SV=1[more]
Q96A467.4e-4737.82Mitoferrin-2 OS=Homo sapiens OX=9606 GN=SLC25A28 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CQJ61.6e-16490.79mitoferrin-like OS=Momordica charantia OX=3673 GN=LOC111013836 PE=3 SV=1[more]
A0A5A7T6D31.5e-16291.00Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G0019... [more]
A0A1S3CL001.5e-16291.00mitoferrin-like OS=Cucumis melo OX=3656 GN=LOC103502157 PE=3 SV=1[more]
A0A5D3CHC53.6e-16190.35Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G0015... [more]
A0A0A0L0066.1e-16190.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G312860 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G07030.13.8e-12371.06Mitochondrial substrate carrier family protein [more]
AT2G30160.18.2e-11867.52Mitochondrial substrate carrier family protein [more]
AT1G34065.16.3e-3331.71S-adenosylmethionine carrier 2 [more]
AT1G74240.11.4e-3230.17Mitochondrial substrate carrier family protein [more]
AT4G39460.11.0e-3032.49S-adenosylmethionine carrier 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002067Mitochondrial carrier proteinPRINTSPR00926MITOCARRIERcoord: 130..148
score: 26.57
coord: 172..190
score: 23.55
coord: 30..43
score: 22.48
coord: 220..242
score: 27.51
coord: 43..57
score: 28.42
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 26..109
e-value: 8.2E-20
score: 70.5
coord: 211..304
e-value: 1.4E-21
score: 76.2
coord: 116..201
e-value: 1.2E-19
score: 70.1
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 115..199
score: 23.714384
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 25..108
score: 17.60025
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 211..301
score: 20.796762
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 110..310
e-value: 6.1E-52
score: 178.2
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 25..296
NoneNo IPR availablePANTHERPTHR45758:SF12MITOFERRIN-LIKEcoord: 2..309
NoneNo IPR availablePANTHERPTHR45758MITOFERRIN-1-RELATEDcoord: 2..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012324.1Lag0012324.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048250 iron import into the mitochondrion
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005381 iron ion transmembrane transporter activity