Lag0012319 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012319 vs. NCBI nr
Match: KAG7033751.1 (Protein DETOXIFICATION 44, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 889.4 bits (2297), Expect = 1.6e-254
Identity = 498/591 (84.26%), Postives = 523/591 (88.49%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VPCFNT TNLSSKCQ+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    SASVSVPLLNR RDA FKFD+LA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+ T+
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN  Q N DGIEEN+GKKLLSSVSTSLALA GLGIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISF+LSSIDGGR+ARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSG---------------- 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFS DAEVLEIARSG                
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWEDPVISHDATYTHY 480

Query: 481 --------------LLFVAGSQPVNALAFVVDGLYYGVSDFAYAAYSMMLVGLFSSLYLV 540
                         L FVAGSQPVNALAFVVDGLYYGVSDF YAAYSM+LVGL SSLYL+
Sbjct: 481 KAIVIGCPDILAMFLQFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLL 540

Query: 541 VVTPAFGLPGVWSGLFLFMMLRLVAGIWSAG---HFDATVPEHVRQFAAIF 555
           VVTPAFGLPGVWSGLFLFMMLRL+AGIW AG       TVPEHVRQ AAIF
Sbjct: 541 VVTPAFGLPGVWSGLFLFMMLRLIAGIWRAGPPSMQQPTVPEHVRQSAAIF 591

BLAST of Lag0012319 vs. NCBI nr
Match: XP_022949803.1 (protein DETOXIFICATION 44, chloroplastic [Cucurbita moschata])

HSP 1 Score: 888.3 bits (2294), Expect = 3.5e-254
Identity = 485/541 (89.65%), Postives = 510/541 (94.27%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VPCFNT TNLSSKCQ+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    SASVSVPLLNR RDA FKFD+LA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+ T+
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN  Q N DGIEEN+GKKLLSSVSTSLALA GLGIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISF+LSSIDGGR+ARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFS DAEVLEIARSGL FVAGSQPVNALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+LVGL SSLYL+VVTPAFGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

BLAST of Lag0012319 vs. NCBI nr
Match: XP_023543949.1 (protein DETOXIFICATION 44, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023543950.1 protein DETOXIFICATION 44, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023543951.1 protein DETOXIFICATION 44, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.9 bits (2288), Expect = 1.8e-253
Identity = 486/541 (89.83%), Postives = 509/541 (94.09%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VP FNT TNLSSKCQ+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPSFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    SASVSVPLLNR RDAVFKFDKLA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+ T+
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN  Q N DGIEEN+GKKLLSSVSTSLALA GLGIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISF+LSSIDGGRIARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+V MAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVSMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFS DAEVLEIARSGL FVAGSQPVNALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+LVGL SSLYL+VVTPAFGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

BLAST of Lag0012319 vs. NCBI nr
Match: XP_022978135.1 (protein DETOXIFICATION 44, chloroplastic [Cucurbita maxima])

HSP 1 Score: 884.4 bits (2284), Expect = 5.1e-253
Identity = 484/541 (89.46%), Postives = 508/541 (93.90%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VPCFNT TNLSSKCQ+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    S  VSVPLLNR RDAVFKFDKLA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+  +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN VQ N DGIEEN+GKKLLSSVSTSLALA G+GIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS +LSSIDGGRIARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFS DAEVLEIARSGL FVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+LVGL SSLYL+VVTPAFGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

BLAST of Lag0012319 vs. NCBI nr
Match: KAG6603561.1 (Protein DETOXIFICATION 44, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 883.6 bits (2282), Expect = 8.7e-253
Identity = 484/541 (89.46%), Postives = 509/541 (94.09%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VPCFNT TNLSSK Q+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKRQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    SASVSVPLLNR RDA FKFD+LA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+ T+
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN  Q N DGIEEN+GKKLLSSVSTSLALA GLGIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISF+LSSIDGGR+ARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFS DAEVLEIARSGL FVAGSQPVNALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+LVGL SSLYL+VVTPAFGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

BLAST of Lag0012319 vs. ExPASy Swiss-Prot
Match: Q84K71 (Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX44 PE=2 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.5e-154
Identity = 310/500 (62.00%), Postives = 383/500 (76.60%), Query Frame = 0

Query: 39  PKASFQKNLTTSSLKSPPEESKSTVSSEQVRRNKPDVSSNS-ASVSVPLLNRLRDAVFKF 98
           P +S ++ +   S  SP +ES +  +S Q    +P+   N   S + P  +   D     
Sbjct: 22  PNSSIRRTIVCKS--SPRDESPAVSTSSQ----RPEKQQNPLTSQNKPDHDHKPDP--GI 81

Query: 99  DKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVP 158
            K+ M++++IALPAALALAADPI SLVDTAFVGHIGS ELAAVGVS SVFNLVSKLFNVP
Sbjct: 82  GKIGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVP 141

Query: 159 LLNITTSFVAEEQALMSTDDKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVM 218
           LLN+TTSFVAEEQA+ + DD + ++T+         KK+L SVSTSL LA G+GIAEA+ 
Sbjct: 142 LLNVTTSFVAEEQAIAAKDDNDSIETS---------KKVLPSVSTSLVLAAGVGIAEAIA 201

Query: 219 LSLGSGALMDIMGIPVDSSMRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYA 278
           LSLGS  LMD+M IP DS MR PAEQFL LRAYGAPPIV+ALAAQG +RGFKDT TPLYA
Sbjct: 202 LSLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYA 261

Query: 279 TAAGNLLNAVLDPLLIFLCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGR 338
             AGN+LNAVLDP+LIF+ GFGI GAA ATVISEYLIAF+LLW+LN  +      I  GR
Sbjct: 262 VVAGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGR 321

Query: 339 IARYLQSGGLLMGRTLAVLVTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALA 398
             +YL+SGGLL+GRT+A+LV  TLATS+AA+ G   MAG+QI ++IWLA+SLLTDALA+A
Sbjct: 322 ANQYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIA 381

Query: 399 AQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIA 458
           AQ+LLA++Y+  +Y+ +R+V++  LQ+GL +G  LA +LF+ F  FS LF+ D+EVL+IA
Sbjct: 382 AQSLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVLKIA 441

Query: 459 RSGLLFVAGSQPVNALAFVVDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGV 518
            SG LFVAGSQPVNALAFV+DGLYYGVSDF +AAYSM++VG  SSL+++V  P FGL G+
Sbjct: 442 LSGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFMLVAAPTFGLAGI 501

Query: 519 WSGLFLFMMLRLVAGIWSAG 538
           W+GLFLFM LRLVAG W  G
Sbjct: 502 WTGLFLFMALRLVAGAWRLG 504

BLAST of Lag0012319 vs. ExPASy Swiss-Prot
Match: Q9SVE7 (Protein DETOXIFICATION 45, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX45 PE=2 SV=2)

HSP 1 Score: 392.1 bits (1006), Expect = 1.0e-107
Identity = 230/478 (48.12%), Postives = 322/478 (67.36%), Query Frame = 0

Query: 68  VRRNKPDVSSNSASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALAADPIASLVDTA 127
           V+  + D S +S    +P +N +   V +   +  +++ ++LPA    A DP+  L++TA
Sbjct: 74  VKLGEEDDSCSSLD-KLPEVNGVHTGVARPVDIKRELVMLSLPAIAGQAIDPLTLLMETA 133

Query: 128 FVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTDDKNIVQTNTD- 187
           ++G +GS EL + GVS ++FN +SKLFN+PLL++ TSFVAE+ A ++  D     + +D 
Sbjct: 134 YIGRLGSVELGSAGVSMAIFNTISKLFNIPLLSVATSFVAEDIAKIAAQDLASEDSQSDI 193

Query: 188 ---GIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSMRAPAEQ 247
              G+ E   +K LSSVST+L LA G+GI EA+ LSL SG  + +MGI   S M  PA Q
Sbjct: 194 PSQGLPE---RKQLSSVSTALVLAIGIGIFEALALSLASGPFLRLMGIQSMSEMFIPARQ 253

Query: 248 FLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCGFGIGGA 307
           FL LRA GAP  V++LA QG +RGFKDTKTP+Y    GN L   L PL I+    G+ GA
Sbjct: 254 FLVLRALGAPAYVVSLALQGIFRGFKDTKTPVYCLGIGNFLAVFLFPLFIYKFRMGVAGA 313

Query: 308 AIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLVTLTLAT 367
           AI++VIS+Y +A ++L  LN  +      I   +   YL+SGG ++GRTL+VLVT+T+AT
Sbjct: 314 AISSVISQYTVAILMLILLNKRVILLPPKIGSLKFGDYLKSGGFVLGRTLSVLVTMTVAT 373

Query: 368 SMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQVIYRTLQ 427
           SMAAR+G   MA +QIC+Q+WLA+SLLTDALA + QAL+ASS + +D+E  ++V    L+
Sbjct: 374 SMAARQGVFAMAAHQICMQVWLAVSLLTDALASSGQALIASSASKRDFEGVKEVTTFVLK 433

Query: 428 IGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVVDGLYYG 487
           IG+++GI+LAI+L + F + +GLFS D EVL I R G+LFVA +QP+ ALAF+ DGL+YG
Sbjct: 434 IGVVTGIALAIVLGMSFSSIAGLFSKDPEVLRIVRKGVLFVAATQPITALAFIFDGLHYG 493

Query: 488 VSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAG----IWSAG 538
           +SDF YAA SMM+VG  SS +++      GL GVW GL +FM LR+VAG    +W  G
Sbjct: 494 MSDFPYAACSMMVVGGISSAFMLYAPAGLGLSGVWVGLSMFMGLRMVAGFSRLMWRKG 547

BLAST of Lag0012319 vs. ExPASy Swiss-Prot
Match: Q9SYD6 (Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2)

HSP 1 Score: 358.2 bits (918), Expect = 1.7e-97
Identity = 210/480 (43.75%), Postives = 303/480 (63.12%), Query Frame = 0

Query: 93  AVFKFDKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSK 152
           +V KFD+L +++  IALPAALAL ADPIASLVDTAF+G IG  ELAAVGVS ++FN VS+
Sbjct: 25  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 84

Query: 153 LFNVPLLNITTSFVAEEQALMSTDD---------------------KNIVQTNTDGIEEN 212
           +   PL++ITTSFVAEE A  S  D                     + I + + D + + 
Sbjct: 85  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 144

Query: 213 --------------RGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSM 272
                           K+ + S S++L +   LG+ +AV L   +  L+  MG+  DS M
Sbjct: 145 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 204

Query: 273 RAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCG 332
             P++++LSLR+ GAP ++++LAAQG +RGFKDT TPL+AT  G++ N +LDP+ IF+  
Sbjct: 205 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 264

Query: 333 FGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLV 392
            G+ GAA A VIS+YL+  +LLW+L G++     S    +  R++++G LL+ R +AV  
Sbjct: 265 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 324

Query: 393 TLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQV 452
            +TL+ S+AAREGS  MA +Q+C+Q+WLA SLL D  A+A QA+LAS++  +DY+ +   
Sbjct: 325 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 384

Query: 453 IYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVV 512
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL +   GL FVAG+QP+NALAFV 
Sbjct: 385 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 444

Query: 513 DGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSAG 538
           DG+ +G SDF YAA S+++V + S L L+ ++   G  G+W GL ++M LR   G W  G
Sbjct: 445 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 504

BLAST of Lag0012319 vs. ExPASy Swiss-Prot
Match: Q9SFB0 (Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 3.4e-90
Identity = 201/490 (41.02%), Postives = 298/490 (60.82%), Query Frame = 0

Query: 84  VPLLNRLRDA--VFKFDKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVG 143
           +P L   +D   VF  D    ++L IA PAALALAADPIASL+DTAFVG +G+ +LAAVG
Sbjct: 15  IPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLAAVG 74

Query: 144 VSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTDDK----NIVQTNT----DGIEE--- 203
           VS ++FN  S++   PL+++TTSFVAEE  +    ++    N+V   T    D +E+   
Sbjct: 75  VSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEKGIS 134

Query: 204 ---------------------------NRGKKLLSSVSTSLALATGLGIAEAVMLSLGSG 263
                                       + K+ + + ST++ L   LG+ +A+ L   S 
Sbjct: 135 SPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSK 194

Query: 264 ALMDIMGIPVDSSMRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNL 323
            L+ +MG+  +S M +PA ++LS+RA GAP ++++LA QG +RGFKDTKTPL+AT   ++
Sbjct: 195 LLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADV 254

Query: 324 LNAVLDPLLIFLCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQ 383
           +N VLDP+ IF+   GI GAAIA VIS+Y +  +L   L  +++    +    +  R+L+
Sbjct: 255 INIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLK 314

Query: 384 SGGLLMGRTLAVLVTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLA 443
           +G LL+ RT+AV    TLA +MAAR G+ PMA +QIC+Q+WL  SLL D LA+A QA+LA
Sbjct: 315 NGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILA 374

Query: 444 SSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLF 503
            S+  +DY     V  R LQ+G + G+ L++ + LG    +G+FS D  V+ +   G+ F
Sbjct: 375 CSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPF 434

Query: 504 VAGSQPVNALAFVVDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFL 534
           +A +QP+N+LAFV+DG+ +G SDFAY AYSM+ V   S   ++ +    G  G+W  L +
Sbjct: 435 IAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIALTI 494

BLAST of Lag0012319 vs. ExPASy Swiss-Prot
Match: Q8W4G3 (Protein DETOXIFICATION 46, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX46 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.3e-12
Identity = 102/425 (24.00%), Postives = 183/425 (43.06%), Query Frame = 0

Query: 110 PAALALAADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEE 169
           PAA      P+ SL+DTA +G   S ELAA+G +  + + +   F    L++ TS +   
Sbjct: 125 PAAGLWLCGPLMSLIDTAVIGQGSSLELAALGPATVICDYLCYTF--MFLSVATSNLVAT 184

Query: 170 QALMSTDDKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSL-GSGALMDI 229
              ++  DK+ VQ                 +S  L +    G+   V+  L GS AL   
Sbjct: 185 S--LARQDKDEVQ---------------HQISILLFIGLACGVTMMVLTRLFGSWALTAF 244

Query: 230 MGIPVDSSMRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVL 289
            G+  ++ +   A +++ +R    P ++I   AQ    G KD+  PL A A  + +N V 
Sbjct: 245 TGVK-NADIVPAANKYVQIRGLAWPAVLIGWVAQSASLGMKDSWGPLKALAVASAINGVG 304

Query: 290 DPLLIFLCGFGIGGAAIATVISEYLIAFVLLWRLN--GEISFSLSSIDGGRIARYLQSGG 349
           D +L    G+GI GAA AT++S+ + A++++  LN  G  +FS        +        
Sbjct: 305 DVVLCTFLGYGIAGAAWATMVSQVVAAYMMMDALNKKGYSAFSFCVPSPSELLTIFGLAA 364

Query: 350 LLMGRTLAVLVTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSY 409
            +    ++ ++  TL    A   G+  +A +Q+ +QI+   ++  + L+  AQ+ +    
Sbjct: 365 PVFITMMSKVLFYTLLVYFATSMGTNIIAAHQVMLQIYTMSTVWGEPLSQTAQSFMPELL 424

Query: 410 --TLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFV 469
               ++   +R ++   + IG   GI +  I       F G+F+ D  V       ++  
Sbjct: 425 FGINRNLPKARVLLKSLVIIGATLGIVVGTIGTAVPWLFPGIFTRDKVVTSEMHKVIIPY 484

Query: 470 AGSQPVNALAFVVDGLYYGVSDFAYAAYSMM-LVGLFSSLYLVVVTPAFGLPGVWSGLFL 529
             +  +      ++G      D  Y + SM   + +   L +++    FGL G W  L  
Sbjct: 485 FLALSITPSTHSLEGTLLAGRDLRYISLSMTGCLAVAGLLLMLLSNGGFGLRGCWYALVG 529

BLAST of Lag0012319 vs. ExPASy TrEMBL
Match: A0A6J1GD47 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453089 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 485/541 (89.65%), Postives = 510/541 (94.27%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VPCFNT TNLSSKCQ+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    SASVSVPLLNR RDA FKFD+LA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+ T+
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN  Q N DGIEEN+GKKLLSSVSTSLALA GLGIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISF+LSSIDGGR+ARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFS DAEVLEIARSGL FVAGSQPVNALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+LVGL SSLYL+VVTPAFGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

BLAST of Lag0012319 vs. ExPASy TrEMBL
Match: A0A6J1IP82 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478206 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.5e-253
Identity = 484/541 (89.46%), Postives = 508/541 (93.90%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+VPCFNT TNLSSKCQ+LRRNANC++RFRY  KASFQKNLTTSSLKSPPEE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STVSSEQVRRNKPDVSSN----SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           ST SSEQ+RRN P   S+    S  VSVPLLNR RDAVFKFDKLA+D+LAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL+  +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +KN VQ N DGIEEN+GKKLLSSVSTSLALA G+GIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNAVLDPLLIF C
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS +LSSIDGGRIARYL+SGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG+VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFS DAEVLEIARSGL FVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+LVGL SSLYL+VVTPAFGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

BLAST of Lag0012319 vs. ExPASy TrEMBL
Match: A0A6J1CS71 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111013829 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.9e-247
Identity = 477/541 (88.17%), Postives = 506/541 (93.53%), Query Frame = 0

Query: 1   MATSLS-IHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEES 60
           MA +LS I+VPCF+TDTNLSSK QMLRRNANCK+RFR LPKA FQKN TT SLKSPPEE 
Sbjct: 1   MAAALSAIYVPCFSTDTNLSSKIQMLRRNANCKVRFRSLPKALFQKNFTT-SLKSPPEEP 60

Query: 61  KSTVSSEQVRRNKPDVSSN---SASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALA 120
           KS+ SSEQVRR+KP  SSN   SA VSVPLLNR+RDAVFKFD+LA+D+LAIALPAALALA
Sbjct: 61  KSSASSEQVRRDKPAKSSNSLLSAPVSVPLLNRVRDAVFKFDQLALDVLAIALPAALALA 120

Query: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTD 180
           ADPIASLVDTAFVGHIGSTELAAVGVSAS+FNLVSKLFNVPLLNITTSFVAEEQAL+ST 
Sbjct: 121 ADPIASLVDTAFVGHIGSTELAAVGVSASIFNLVSKLFNVPLLNITTSFVAEEQALVSTK 180

Query: 181 DKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSS 240
           +K+IVQTN DGI+EN+ KKLLSSVSTSLALA GLGIAEAVML LGSGALMDIMGIP +SS
Sbjct: 181 EKSIVQTNIDGIDENQEKKLLSSVSTSLALAAGLGIAEAVMLCLGSGALMDIMGIPAESS 240

Query: 241 MRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLC 300
           MRAPAEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNA LDPLLIFLC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAALDPLLIFLC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLW+LNGEISF+LSS+DG RIARYLQSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWKLNGEISFTLSSMDGERIARYLQSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG VPMAGYQICVQIWLAISLLTDALALA QALLASSYTLQDYEHSR+
Sbjct: 361 VTLTLATSMAAREGPVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRE 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFV 480
           VIYR LQIGLI+GISLAIILFLGFGAF+GLFS DAEVLEIARSGL FVAGSQPVNA+AFV
Sbjct: 421 VIYRVLQIGLITGISLAIILFLGFGAFAGLFSADAEVLEIARSGLFFVAGSQPVNAMAFV 480

Query: 481 VDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSA 538
           VDGLYYGVSDF YAAYSM+ VGL SSL+L+V  PAFGLPGVWSGLFLFMMLRLVAGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVAVGLVSSLFLIVAAPAFGLPGVWSGLFLFMMLRLVAGIWRL 540

BLAST of Lag0012319 vs. ExPASy TrEMBL
Match: A0A5D3CIE7 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00260 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.4e-242
Identity = 466/535 (87.10%), Postives = 497/535 (92.90%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+ P FNT TNLS KCQMLRRNANC L F YLPK SF KNLTTSSLKSP EESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STVSSEQVRRNKPDVSSNSASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALAADPI 120
           ST SS+QVRRN+ D +S+++ +S   +NR RDA FKFD+LA+D+LAIALPAALALAADPI
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSAS-VNRFRDAAFKFDELALDILAIALPAALALAADPI 120

Query: 121 ASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTDDKNI 180
           ASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL++TD+KNI
Sbjct: 121 ASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNI 180

Query: 181 VQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSMRAP 240
           VQT+ D IE+ + KKLLSSVSTSLALAT LGIAEAVMLSLGSG LMDIMGIPVDSSMRAP
Sbjct: 181 VQTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAP 240

Query: 241 AEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCGFGI 300
           AEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGNLLNA+LDPLLIF CGFGI
Sbjct: 241 AEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAILDPLLIFFCGFGI 300

Query: 301 GGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLVTLT 360
           GGAAIATVISEYLIAF+LLWRLNGEISF+LSSIDGGRIARYLQSGGLLM RTLAVLVTLT
Sbjct: 301 GGAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLT 360

Query: 361 LATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQVIYR 420
           LATSMAAREG VPMAGYQI VQIW+AISLLTDALALA QALLA S+TLQDY+HSRQVIYR
Sbjct: 361 LATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQVIYR 420

Query: 421 TLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVVDGL 480
           TLQIGLISGISLAIILFLGFGAFSGLFS DAEVLE ARSG LFVAGSQPVNALAFVVDGL
Sbjct: 421 TLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGL 480

Query: 481 YYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWS 536
           YYGVSDF YAAYSM+LVGL SS++L+VVTP FGLPGVWSGLFLFMMLRLVAGIWS
Sbjct: 481 YYGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWS 534

BLAST of Lag0012319 vs. ExPASy TrEMBL
Match: A0A1S3CL06 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103502163 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 7.5e-242
Identity = 465/537 (86.59%), Postives = 496/537 (92.36%), Query Frame = 0

Query: 1   MATSLSIHVPCFNTDTNLSSKCQMLRRNANCKLRFRYLPKASFQKNLTTSSLKSPPEESK 60
           MAT LSI+ P FNT TNLS KCQMLRRNANC L F YLPK SF KNLTTSSLKSP EESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STVSSEQVRRNKPDVSSNSASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALAADPI 120
           ST SS+QVRRN+ D +S+++ +S   +NR RDA FKFD+LA+D+LAIALPAALALAADPI
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSAS-VNRFRDAAFKFDELALDILAIALPAALALAADPI 120

Query: 121 ASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTDDKNI 180
           ASL+DTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQAL++TD+KNI
Sbjct: 121 ASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNI 180

Query: 181 VQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSMRAP 240
           VQT+ D IE+ + KKLLSSVSTSLALAT LGIAEAVMLSLGSG LMDIMGIPVDSSMRAP
Sbjct: 181 VQTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAP 240

Query: 241 AEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCGFGI 300
           AEQFLSLRA+GAPPIVIALAAQGT+RGFKDTKTPLYATAAGN LNA+LDPLLIF CGFGI
Sbjct: 241 AEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNFLNAILDPLLIFFCGFGI 300

Query: 301 GGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLVTLT 360
           GGAAIATVISEYLIAF+LLWRLNGEISF+LSSIDGGRIARYLQSGGLLM RTLAVLVTLT
Sbjct: 301 GGAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLT 360

Query: 361 LATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQVIYR 420
           LATSMAAREG VPMAGYQI VQIW+AISLLTDALALA QALLA S+TLQDY+HSRQVIYR
Sbjct: 361 LATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQVIYR 420

Query: 421 TLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVVDGL 480
           TLQIGLISGISLAIILFLGFGAFSGLFS DAEVLE ARSG LFVAGSQPVNALAFVVDGL
Sbjct: 421 TLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGL 480

Query: 481 YYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSAG 538
           YYGVSDF YAAYSM+LVGL SS++L+VVTP FGLPGVWSGLFLFMMLRLVAGIW  G
Sbjct: 481 YYGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWRLG 536

BLAST of Lag0012319 vs. TAIR 10
Match: AT2G38330.1 (MATE efflux family protein )

HSP 1 Score: 547.7 bits (1410), Expect = 1.1e-155
Identity = 310/500 (62.00%), Postives = 383/500 (76.60%), Query Frame = 0

Query: 39  PKASFQKNLTTSSLKSPPEESKSTVSSEQVRRNKPDVSSNS-ASVSVPLLNRLRDAVFKF 98
           P +S ++ +   S  SP +ES +  +S Q    +P+   N   S + P  +   D     
Sbjct: 22  PNSSIRRTIVCKS--SPRDESPAVSTSSQ----RPEKQQNPLTSQNKPDHDHKPDP--GI 81

Query: 99  DKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVP 158
            K+ M++++IALPAALALAADPI SLVDTAFVGHIGS ELAAVGVS SVFNLVSKLFNVP
Sbjct: 82  GKIGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVP 141

Query: 159 LLNITTSFVAEEQALMSTDDKNIVQTNTDGIEENRGKKLLSSVSTSLALATGLGIAEAVM 218
           LLN+TTSFVAEEQA+ + DD + ++T+         KK+L SVSTSL LA G+GIAEA+ 
Sbjct: 142 LLNVTTSFVAEEQAIAAKDDNDSIETS---------KKVLPSVSTSLVLAAGVGIAEAIA 201

Query: 219 LSLGSGALMDIMGIPVDSSMRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYA 278
           LSLGS  LMD+M IP DS MR PAEQFL LRAYGAPPIV+ALAAQG +RGFKDT TPLYA
Sbjct: 202 LSLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYA 261

Query: 279 TAAGNLLNAVLDPLLIFLCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGR 338
             AGN+LNAVLDP+LIF+ GFGI GAA ATVISEYLIAF+LLW+LN  +      I  GR
Sbjct: 262 VVAGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGR 321

Query: 339 IARYLQSGGLLMGRTLAVLVTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALA 398
             +YL+SGGLL+GRT+A+LV  TLATS+AA+ G   MAG+QI ++IWLA+SLLTDALA+A
Sbjct: 322 ANQYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIA 381

Query: 399 AQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIA 458
           AQ+LLA++Y+  +Y+ +R+V++  LQ+GL +G  LA +LF+ F  FS LF+ D+EVL+IA
Sbjct: 382 AQSLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVLKIA 441

Query: 459 RSGLLFVAGSQPVNALAFVVDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGV 518
            SG LFVAGSQPVNALAFV+DGLYYGVSDF +AAYSM++VG  SSL+++V  P FGL G+
Sbjct: 442 LSGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFMLVAAPTFGLAGI 501

Query: 519 WSGLFLFMMLRLVAGIWSAG 538
           W+GLFLFM LRLVAG W  G
Sbjct: 502 WTGLFLFMALRLVAGAWRLG 504

BLAST of Lag0012319 vs. TAIR 10
Match: AT4G38380.1 (MATE efflux family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 7.4e-109
Identity = 230/478 (48.12%), Postives = 322/478 (67.36%), Query Frame = 0

Query: 68  VRRNKPDVSSNSASVSVPLLNRLRDAVFKFDKLAMDMLAIALPAALALAADPIASLVDTA 127
           V+  + D S +S    +P +N +   V +   +  +++ ++LPA    A DP+  L++TA
Sbjct: 74  VKLGEEDDSCSSLD-KLPEVNGVHTGVARPVDIKRELVMLSLPAIAGQAIDPLTLLMETA 133

Query: 128 FVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTDDKNIVQTNTD- 187
           ++G +GS EL + GVS ++FN +SKLFN+PLL++ TSFVAE+ A ++  D     + +D 
Sbjct: 134 YIGRLGSVELGSAGVSMAIFNTISKLFNIPLLSVATSFVAEDIAKIAAQDLASEDSQSDI 193

Query: 188 ---GIEENRGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSMRAPAEQ 247
              G+ E   +K LSSVST+L LA G+GI EA+ LSL SG  + +MGI   S M  PA Q
Sbjct: 194 PSQGLPE---RKQLSSVSTALVLAIGIGIFEALALSLASGPFLRLMGIQSMSEMFIPARQ 253

Query: 248 FLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCGFGIGGA 307
           FL LRA GAP  V++LA QG +RGFKDTKTP+Y    GN L   L PL I+    G+ GA
Sbjct: 254 FLVLRALGAPAYVVSLALQGIFRGFKDTKTPVYCLGIGNFLAVFLFPLFIYKFRMGVAGA 313

Query: 308 AIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLVTLTLAT 367
           AI++VIS+Y +A ++L  LN  +      I   +   YL+SGG ++GRTL+VLVT+T+AT
Sbjct: 314 AISSVISQYTVAILMLILLNKRVILLPPKIGSLKFGDYLKSGGFVLGRTLSVLVTMTVAT 373

Query: 368 SMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQVIYRTLQ 427
           SMAAR+G   MA +QIC+Q+WLA+SLLTDALA + QAL+ASS + +D+E  ++V    L+
Sbjct: 374 SMAARQGVFAMAAHQICMQVWLAVSLLTDALASSGQALIASSASKRDFEGVKEVTTFVLK 433

Query: 428 IGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVVDGLYYG 487
           IG+++GI+LAI+L + F + +GLFS D EVL I R G+LFVA +QP+ ALAF+ DGL+YG
Sbjct: 434 IGVVTGIALAIVLGMSFSSIAGLFSKDPEVLRIVRKGVLFVAATQPITALAFIFDGLHYG 493

Query: 488 VSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAG----IWSAG 538
           +SDF YAA SMM+VG  SS +++      GL GVW GL +FM LR+VAG    +W  G
Sbjct: 494 MSDFPYAACSMMVVGGISSAFMLYAPAGLGLSGVWVGLSMFMGLRMVAGFSRLMWRKG 547

BLAST of Lag0012319 vs. TAIR 10
Match: AT1G51340.2 (MATE efflux family protein )

HSP 1 Score: 358.2 bits (918), Expect = 1.2e-98
Identity = 210/480 (43.75%), Postives = 303/480 (63.12%), Query Frame = 0

Query: 93  AVFKFDKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSK 152
           +V KFD+L +++  IALPAALAL ADPIASLVDTAF+G IG  ELAAVGVS ++FN VS+
Sbjct: 25  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 84

Query: 153 LFNVPLLNITTSFVAEEQALMSTDD---------------------KNIVQTNTDGIEEN 212
           +   PL++ITTSFVAEE A  S  D                     + I + + D + + 
Sbjct: 85  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 144

Query: 213 --------------RGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSM 272
                           K+ + S S++L +   LG+ +AV L   +  L+  MG+  DS M
Sbjct: 145 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 204

Query: 273 RAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCG 332
             P++++LSLR+ GAP ++++LAAQG +RGFKDT TPL+AT  G++ N +LDP+ IF+  
Sbjct: 205 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 264

Query: 333 FGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLV 392
            G+ GAA A VIS+YL+  +LLW+L G++     S    +  R++++G LL+ R +AV  
Sbjct: 265 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 324

Query: 393 TLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQV 452
            +TL+ S+AAREGS  MA +Q+C+Q+WLA SLL D  A+A QA+LAS++  +DY+ +   
Sbjct: 325 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 384

Query: 453 IYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVV 512
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL +   GL FVAG+QP+NALAFV 
Sbjct: 385 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 444

Query: 513 DGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSAG 538
           DG+ +G SDF YAA S+++V + S L L+ ++   G  G+W GL ++M LR   G W  G
Sbjct: 445 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 504

BLAST of Lag0012319 vs. TAIR 10
Match: AT1G51340.1 (MATE efflux family protein )

HSP 1 Score: 358.2 bits (918), Expect = 1.2e-98
Identity = 210/480 (43.75%), Postives = 303/480 (63.12%), Query Frame = 0

Query: 93  AVFKFDKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVGVSASVFNLVSK 152
           +V KFD+L +++  IALPAALAL ADPIASLVDTAF+G IG  ELAAVGVS ++FN VS+
Sbjct: 19  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 78

Query: 153 LFNVPLLNITTSFVAEEQALMSTDD---------------------KNIVQTNTDGIEEN 212
           +   PL++ITTSFVAEE A  S  D                     + I + + D + + 
Sbjct: 79  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 138

Query: 213 --------------RGKKLLSSVSTSLALATGLGIAEAVMLSLGSGALMDIMGIPVDSSM 272
                           K+ + S S++L +   LG+ +AV L   +  L+  MG+  DS M
Sbjct: 139 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 198

Query: 273 RAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNLLNAVLDPLLIFLCG 332
             P++++LSLR+ GAP ++++LAAQG +RGFKDT TPL+AT  G++ N +LDP+ IF+  
Sbjct: 199 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 258

Query: 333 FGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQSGGLLMGRTLAVLV 392
            G+ GAA A VIS+YL+  +LLW+L G++     S    +  R++++G LL+ R +AV  
Sbjct: 259 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 318

Query: 393 TLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLASSYTLQDYEHSRQV 452
            +TL+ S+AAREGS  MA +Q+C+Q+WLA SLL D  A+A QA+LAS++  +DY+ +   
Sbjct: 319 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 378

Query: 453 IYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLFVAGSQPVNALAFVV 512
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL +   GL FVAG+QP+NALAFV 
Sbjct: 379 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 438

Query: 513 DGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFLFMMLRLVAGIWSAG 538
           DG+ +G SDF YAA S+++V + S L L+ ++   G  G+W GL ++M LR   G W  G
Sbjct: 439 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 498

BLAST of Lag0012319 vs. TAIR 10
Match: AT3G08040.1 (MATE efflux family protein )

HSP 1 Score: 334.0 bits (855), Expect = 2.4e-91
Identity = 201/490 (41.02%), Postives = 298/490 (60.82%), Query Frame = 0

Query: 84  VPLLNRLRDA--VFKFDKLAMDMLAIALPAALALAADPIASLVDTAFVGHIGSTELAAVG 143
           +P L   +D   VF  D    ++L IA PAALALAADPIASL+DTAFVG +G+ +LAAVG
Sbjct: 15  IPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLAAVG 74

Query: 144 VSASVFNLVSKLFNVPLLNITTSFVAEEQALMSTDDK----NIVQTNT----DGIEE--- 203
           VS ++FN  S++   PL+++TTSFVAEE  +    ++    N+V   T    D +E+   
Sbjct: 75  VSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEKGIS 134

Query: 204 ---------------------------NRGKKLLSSVSTSLALATGLGIAEAVMLSLGSG 263
                                       + K+ + + ST++ L   LG+ +A+ L   S 
Sbjct: 135 SPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSK 194

Query: 264 ALMDIMGIPVDSSMRAPAEQFLSLRAYGAPPIVIALAAQGTYRGFKDTKTPLYATAAGNL 323
            L+ +MG+  +S M +PA ++LS+RA GAP ++++LA QG +RGFKDTKTPL+AT   ++
Sbjct: 195 LLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADV 254

Query: 324 LNAVLDPLLIFLCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFSLSSIDGGRIARYLQ 383
           +N VLDP+ IF+   GI GAAIA VIS+Y +  +L   L  +++    +    +  R+L+
Sbjct: 255 INIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLK 314

Query: 384 SGGLLMGRTLAVLVTLTLATSMAAREGSVPMAGYQICVQIWLAISLLTDALALAAQALLA 443
           +G LL+ RT+AV    TLA +MAAR G+ PMA +QIC+Q+WL  SLL D LA+A QA+LA
Sbjct: 315 NGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILA 374

Query: 444 SSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSDDAEVLEIARSGLLF 503
            S+  +DY     V  R LQ+G + G+ L++ + LG    +G+FS D  V+ +   G+ F
Sbjct: 375 CSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPF 434

Query: 504 VAGSQPVNALAFVVDGLYYGVSDFAYAAYSMMLVGLFSSLYLVVVTPAFGLPGVWSGLFL 534
           +A +QP+N+LAFV+DG+ +G SDFAY AYSM+ V   S   ++ +    G  G+W  L +
Sbjct: 435 IAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIALTI 494