Lag0012272 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012272 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 541/586 (92.32%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GS++VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  QEPIGHRSSGICSFDEMV+YFNQ+DGLSEGY+SGSFNSAFGF GSKQ 
Sbjct: 61  LNVSWDIKNF--QEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+A TK+LS DGFYIPLAKFQ T TP VLQE+VKRA+PTSWDPPSLASFIENFGTHVITS
Sbjct: 121 DAATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSL
Sbjct: 301 LLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPV GMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
            IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 GIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGNK 540
           G RNVLYMKLLYSRLPGCTI+RTLWDHL NDKS+KLVSSS NS NADDSSSGS++IQGNK
Sbjct: 481 GPRNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDNS-NADDSSSGSRNIQGNK 540

Query: 541 LAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVD +EM KGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Lag0012272 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 536/586 (91.47%), Postives = 560/586 (95.56%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTA+NAVQALGRGFDVNFD+RLLYCKGV+GS++VE+DD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  QEPIGHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFNSAFGFTG KQ 
Sbjct: 61  LNVSWDIKNF--QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+AATKSLSTDGFYIPLAKFQ T TP VLQE+VKRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DAAATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIY+KQHQSSPLSTME+KRYVQDIANQRFSDTQGQ GSGSIK KEKGLDGGLF
Sbjct: 181 ITIGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGG+DLEQNHVRWAR VRSSPDVIEMTF PITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAILLYL+YKPPLEELRYFLEFQIPRVWAP  DTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPV GMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGNK 540
           GSRNVLYMKLLYSRLPGCTIRR LWDHLPNDKS+KLV+SS NS+  DDSSSG+KSIQGNK
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRMLWDHLPNDKSKKLVTSSDNSSKVDDSSSGTKSIQGNK 540

Query: 541 LAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAK+VD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of Lag0012272 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 539/587 (91.82%), Postives = 561/587 (95.57%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  Q+PIGHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKNF--QQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+AA K+LSTDGFYIPLAKFQLT+T  +L E+VKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPV GM LSLEG KQNRLSIHLQHLA+LPKILQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN-NADDSSSGSKSIQGN 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGSK+IQGN
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Lag0012272 vs. NCBI nr
Match: XP_023517935.1 (MACPF domain-containing protein CAD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 538/587 (91.65%), Postives = 560/587 (95.40%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  Q+PIGHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKNF--QQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+AA K+LSTDGFYIPLAKFQLT+T  +L E+VKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPV GM LSLEG KQNRLSIHLQHLA+LPKILQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNN-ADDSSSGSKSIQGN 540
           GSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGSK+IQGN
Sbjct: 481 GSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNTAADDSSSGSKNIQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Lag0012272 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 539/588 (91.67%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
            NVS DIKNF  QEPIGHRSSGICSFDEM DYFNQ+DGLSEGY SGSFNSAFGFTG +QT
Sbjct: 61  HNVSWDIKNF--QEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQT 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D++ATKSLSTDGFYIPLAKFQ T TP  L+E++KRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DASATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSL
Sbjct: 301 LLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPV GMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSQKLVSSSVNSNNADDSSSGSKSIQG- 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLP  +KS+KLVSSS N+NNAD+SSSG+KS QG 
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGN 540

Query: 541 NKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           NKLAKLVD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 NKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lag0012272 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 1.9e-200
Identity = 344/584 (58.90%), Postives = 431/584 (73.80%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V +++   RDL L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDS 123
           V  DI   +     G +   +CSF EM + FN R G+      G FN+ F +TGS Q D+
Sbjct: 73  VPADIDCSRGNS--GTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDA 132

Query: 124 AATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSIT 183
           A+TKSL+  G++IPL   +L     VL   ++RAVP+SWDP SLASFIEN+GTH++TS+T
Sbjct: 133 ASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVT 192

Query: 184 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 243
           IGG+DV+Y++QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K         
Sbjct: 193 IGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK--------- 252

Query: 244 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 303
                            D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL
Sbjct: 253 -----------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLL 312

Query: 304 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 363
           +GV G  HLTRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQF
Sbjct: 313 EGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQF 372

Query: 364 SIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVAI 423
           S+MG KL++S +Q+++GRKPV G+RLSLEGSKQNRLSIHLQHL SLPKILQP+WD+HV I
Sbjct: 373 SLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPI 432

Query: 424 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 483
           GAPKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS
Sbjct: 433 GAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGS 492

Query: 484 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGNKLA 543
           +NVL++KLL+S++PGCTIRR++WDH P   S +L     +++++ +  SG    Q  KLA
Sbjct: 493 KNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLA 552

Query: 544 KLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           K+VD +EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 KIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Lag0012272 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 9.2e-115
Identity = 250/603 (41.46%), Postives = 353/603 (58.54%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSS----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           A+ ++G G+D+  D RL YCKG +  S    + E DD    ++ L   + + NVS+ IK 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDD--NCEIVLPGGISIPNVSKSIK- 73

Query: 74  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDSAATKSLS 133
             D+       S I  F +M + FNQ   L+    SG FN+ F F+   Q D+A TK+L+
Sbjct: 74  -CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLA 133

Query: 134 TDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 193
            DG +I L    L  +  +L+EHVK+AVP++WDP +LA FI+ +GTH+I S+ +GGKDVI
Sbjct: 134 FDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVI 193

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFN 253
           Y KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     +
Sbjct: 194 YAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTS 253

Query: 254 NQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITA 313
           + G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+
Sbjct: 254 SLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 313

Query: 314 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPS 373
           LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C S
Sbjct: 314 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 373

Query: 374 LQFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTH 433
           LQFS  G KLYV+   + +G++P+ GMRL LEG + NRL+IHLQHL+SLPKI Q   D +
Sbjct: 374 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 433

Query: 434 VAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWD 493
            +I        E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V  
Sbjct: 434 RSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVES 493

Query: 494 FGSRNVLYMKLLYSRLPGCT-IRRTLWD------------------HLPNDKSQKLVSSS 553
            G +NVL+++L +SR+ G T ++ + WD                  H    +      + 
Sbjct: 494 HGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPAD 553

Query: 554 VNSNNADDSSSGSKSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVK 584
           VN N+A          Q  KL K VD +EM +GPQ+ PG+W+V+GA+L VEKGKI L+VK
Sbjct: 554 VNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVK 590

BLAST of Lag0012272 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.2e-102
Identity = 231/601 (38.44%), Postives = 331/601 (55.07%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSSVVEVDDKHTRDLRLYDDVVLLNVSRDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RDL     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK- 74

Query: 71  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDSAATKSLS 130
             D+       S I SF++M + FNQ   LS    SG FN+ F F+     D+++ K+L+
Sbjct: 75  -CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLA 134

Query: 131 TDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 190
            DG++I L   ++      L++ VKR VP+SWD  +LA FIE +GTHV+  +T+GGKDVI
Sbjct: 135 YDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVI 194

Query: 191 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQP 250
           +VKQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  
Sbjct: 195 HVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFG 254

Query: 251 TSAPYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGV 310
           TS        T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+
Sbjct: 255 TSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGL 314

Query: 311 AGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQFSI 370
            G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+
Sbjct: 315 PGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSL 374

Query: 371 MGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQ-PYWDTHVAIG 430
           MG KLYV+  ++  G +PV G+R  LEG K N L+IHLQHL++ P  L   + DT+  I 
Sbjct: 375 MGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIE 434

Query: 431 APKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSR 490
            P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R
Sbjct: 435 EPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMR 494

Query: 491 NVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGN---- 550
            VL+++L +S       R++ WD+L  +  +  V S +++  +   S    + +      
Sbjct: 495 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID 554

Query: 551 ----------------KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 585
                           KL  LVD  E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSL
Sbjct: 555 INSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSL 603

BLAST of Lag0012272 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.7e-100
Identity = 230/624 (36.86%), Postives = 336/624 (53.85%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSS----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           AV++LG+GFD+  D RL YCK   GS+    +V +D    R+L +    V  NVS DI N
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI-N 71

Query: 74  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGF-TGSKQTDSAATKSL 133
               E    RS  I  F++M +YFNQR  ++    SG+FN+ FGF +GS  TD+A  KSL
Sbjct: 72  CDKGERTRFRSD-ILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 131

Query: 134 STDGFYIPLAKFQLTN-TPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKD 193
             D   + L    + N     L + V+ AVP+SWDP  LA FIE +GTHVIT +++GG+D
Sbjct: 132 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 191

Query: 194 VIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQ---- 253
           V+ V+Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+    ++     
Sbjct: 192 VVVVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPK 251

Query: 254 -----GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMT 313
                 V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   
Sbjct: 252 FPEAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFN 311

Query: 314 FYPITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP--GHQR 373
           F PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV + +P      
Sbjct: 312 FIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPN 371

Query: 374 KEPVCPSLQFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASL-PKI 433
                P+L  + MG KLYV+   ++  + PV GMR  LEG K NRL+IHLQHL +    +
Sbjct: 372 MASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTV 431

Query: 434 LQPYWDTHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG--- 493
            +   D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S    
Sbjct: 432 GEKITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKS 491

Query: 494 ----VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQK------- 553
                +IVTGAQL V   GS++VL+++L Y+++    + +  W H P   SQK       
Sbjct: 492 QNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSM 551

Query: 554 ---LVSSSVNSNNADDSSS-----------GSKSIQGNKLAKLVDMTEMCKGPQDPPGHW 586
              L S SV+ N      +           G      NK+ K VD++++C+GPQ  PGHW
Sbjct: 552 SMPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHW 611

BLAST of Lag0012272 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 541/586 (92.32%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GS++VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  QEPIGHRSSGICSFDEMV+YFNQ+DGLSEGY+SGSFNSAFGF GSKQ 
Sbjct: 61  LNVSWDIKNF--QEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+A TK+LS DGFYIPLAKFQ T TP VLQE+VKRA+PTSWDPPSLASFIENFGTHVITS
Sbjct: 121 DAATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSL
Sbjct: 301 LLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPV GMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
            IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 GIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGNK 540
           G RNVLYMKLLYSRLPGCTI+RTLWDHL NDKS+KLVSSS NS NADDSSSGS++IQGNK
Sbjct: 481 GPRNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDNS-NADDSSSGSRNIQGNK 540

Query: 541 LAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVD +EM KGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Lag0012272 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 539/587 (91.82%), Postives = 561/587 (95.57%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKNF  Q+PIGHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKNF--QQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+AA K+LSTDGFYIPLAKFQLT+T  +L E+VKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPV GM LSLEG KQNRLSIHLQHLA+LPKILQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN-NADDSSSGSKSIQGN 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGSK+IQGN
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Lag0012272 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 539/588 (91.67%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
            NVS DIKNF  QEPIGHRSSGICSFDEM DYFNQ+DGLSEGY SGSFNSAFGFTG +QT
Sbjct: 61  HNVSWDIKNF--QEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQT 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D++ATKSLSTDGFYIPLAKFQ T TP  L+E++KRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DASATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSL
Sbjct: 301 LLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPV GMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSQKLVSSSVNSNNADDSSSGSKSIQG- 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLP  +KS+KLVSSS N+NNAD+SSSG+KS QG 
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGN 540

Query: 541 NKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           NKLAKLVD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 NKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lag0012272 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 539/588 (91.67%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+H+RD+ LYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
            NVS DIKNF  QEPIGHRSSGICSFDEM DYFNQ+DGLSEGY SGSFNSAFGFTG +QT
Sbjct: 61  HNVSWDIKNF--QEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQT 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D++ATKSLSTDGFYIPLAKFQ T TP  L+E++KRAVPT+WDPPSLASFIENFGTHVITS
Sbjct: 121 DASATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA
Sbjct: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSL
Sbjct: 301 LLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQISIGRKPV GMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV
Sbjct: 361 QFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHV 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSQKLVSSSVNSNNADDSSSGSKSIQG- 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLP  +KS+KLVSSS N+NNAD+SSSG+KS QG 
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGN 540

Query: 541 NKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           NKLAKLVD TEM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 NKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lag0012272 vs. ExPASy TrEMBL
Match: A0A6J1HR44 (MACPF domain-containing protein CAD1-like OS=Cucurbita maxima OX=3661 GN=LOC111465969 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 535/587 (91.14%), Postives = 559/587 (95.23%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGS++VEVDD+HTRD+ LYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQT 120
           LNVS DIKN   Q+PIGHRSSGICSFDEMVDYFNQ+DGLSEGY SGSFN+AFGFTGSKQ 
Sbjct: 61  LNVSWDIKN--SQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQI 120

Query: 121 DSAATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITS 180
           D+ A K+LSTDGFYIPLAKFQLT+T  +L E+VKR+VPTSWDPP+LASFIENFGTHVITS
Sbjct: 121 DADAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITS 180

Query: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLF 240
           ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLF
Sbjct: 181 ITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLF 240

Query: 241 NNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITA 300
           NNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPIT+
Sbjct: 241 NNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITS 300

Query: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360
           LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL
Sbjct: 301 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSL 360

Query: 361 QFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHV 420
           QFSIMGQKLYVSIEQI IGRKPV GM LSLEG KQNRLSIHL+HLA+LPKILQPYWDTH+
Sbjct: 361 QFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLKHLATLPKILQPYWDTHI 420

Query: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDF 480
           AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW F
Sbjct: 421 AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGF 480

Query: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSN-NADDSSSGSKSIQGN 540
           GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKS+KLVSSS NSN  ADDSSSGSK+ QGN
Sbjct: 481 GSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNYAADDSSSGSKNTQGN 540

Query: 541 KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVD TEM KGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Lag0012272 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 700.3 bits (1806), Expect = 1.3e-201
Identity = 344/584 (58.90%), Postives = 431/584 (73.80%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSSVVEVDDKHTRDLRLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V +++   RDL L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSRDIKNFQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDS 123
           V  DI   +     G +   +CSF EM + FN R G+      G FN+ F +TGS Q D+
Sbjct: 73  VPADIDCSRGNS--GTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDA 132

Query: 124 AATKSLSTDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSIT 183
           A+TKSL+  G++IPL   +L     VL   ++RAVP+SWDP SLASFIEN+GTH++TS+T
Sbjct: 133 ASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVT 192

Query: 184 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 243
           IGG+DV+Y++QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K         
Sbjct: 193 IGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK--------- 252

Query: 244 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 303
                            D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL
Sbjct: 253 -----------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLL 312

Query: 304 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 363
           +GV G  HLTRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQF
Sbjct: 313 EGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQF 372

Query: 364 SIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVAI 423
           S+MG KL++S +Q+++GRKPV G+RLSLEGSKQNRLSIHLQHL SLPKILQP+WD+HV I
Sbjct: 373 SLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPI 432

Query: 424 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 483
           GAPKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS
Sbjct: 433 GAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGS 492

Query: 484 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGNKLA 543
           +NVL++KLL+S++PGCTIRR++WDH P   S +L     +++++ +  SG    Q  KLA
Sbjct: 493 KNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLA 552

Query: 544 KLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           K+VD +EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 KIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Lag0012272 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 415.6 bits (1067), Expect = 6.5e-116
Identity = 250/603 (41.46%), Postives = 353/603 (58.54%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSS----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           A+ ++G G+D+  D RL YCKG +  S    + E DD    ++ L   + + NVS+ IK 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDD--NCEIVLPGGISIPNVSKSIK- 73

Query: 74  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDSAATKSLS 133
             D+       S I  F +M + FNQ   L+    SG FN+ F F+   Q D+A TK+L+
Sbjct: 74  -CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLA 133

Query: 134 TDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 193
            DG +I L    L  +  +L+EHVK+AVP++WDP +LA FI+ +GTH+I S+ +GGKDVI
Sbjct: 134 FDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVI 193

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFN 253
           Y KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     +
Sbjct: 194 YAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTS 253

Query: 254 NQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITA 313
           + G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+
Sbjct: 254 SLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 313

Query: 314 LLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPS 373
           LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C S
Sbjct: 314 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 373

Query: 374 LQFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTH 433
           LQFS  G KLYV+   + +G++P+ GMRL LEG + NRL+IHLQHL+SLPKI Q   D +
Sbjct: 374 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 433

Query: 434 VAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWD 493
            +I        E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V  
Sbjct: 434 RSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVES 493

Query: 494 FGSRNVLYMKLLYSRLPGCT-IRRTLWD------------------HLPNDKSQKLVSSS 553
            G +NVL+++L +SR+ G T ++ + WD                  H    +      + 
Sbjct: 494 HGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPAD 553

Query: 554 VNSNNADDSSSGSKSIQGNKLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVK 584
           VN N+A          Q  KL K VD +EM +GPQ+ PG+W+V+GA+L VEKGKI L+VK
Sbjct: 554 VNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVK 590

BLAST of Lag0012272 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 373.2 bits (957), Expect = 3.7e-103
Identity = 231/601 (38.44%), Postives = 331/601 (55.07%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSSVVEVDDKHTRDLRLYDDVVLLNVSRDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RDL     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK- 74

Query: 71  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDSAATKSLS 130
             D+       S I SF++M + FNQ   LS    SG FN+ F F+     D+++ K+L+
Sbjct: 75  -CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLA 134

Query: 131 TDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 190
            DG++I L   ++      L++ VKR VP+SWD  +LA FIE +GTHV+  +T+GGKDVI
Sbjct: 135 YDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVI 194

Query: 191 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQP 250
           +VKQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  
Sbjct: 195 HVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFG 254

Query: 251 TSAPYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGV 310
           TS        T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+
Sbjct: 255 TSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGL 314

Query: 311 AGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQFSI 370
            G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+
Sbjct: 315 PGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSL 374

Query: 371 MGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASLPKILQ-PYWDTHVAIG 430
           MG KLYV+  ++  G +PV G+R  LEG K N L+IHLQHL++ P  L   + DT+  I 
Sbjct: 375 MGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIE 434

Query: 431 APKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSR 490
            P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R
Sbjct: 435 EPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMR 494

Query: 491 NVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQKLVSSSVNSNNADDSSSGSKSIQGN---- 550
            VL+++L +S       R++ WD+L  +  +  V S +++  +   S    + +      
Sbjct: 495 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID 554

Query: 551 ----------------KLAKLVDMTEMCKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 585
                           KL  LVD  E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSL
Sbjct: 555 INSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSL 603

BLAST of Lag0012272 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 368.2 bits (944), Expect = 1.2e-101
Identity = 230/624 (36.86%), Postives = 336/624 (53.85%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSS----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           AV++LG+GFD+  D RL YCK   GS+    +V +D    R+L +    V  NVS DI N
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI-N 71

Query: 74  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGF-TGSKQTDSAATKSL 133
               E    RS  I  F++M +YFNQR  ++    SG+FN+ FGF +GS  TD+A  KSL
Sbjct: 72  CDKGERTRFRSD-ILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 131

Query: 134 STDGFYIPLAKFQLTN-TPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKD 193
             D   + L    + N     L + V+ AVP+SWDP  LA FIE +GTHVIT +++GG+D
Sbjct: 132 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 191

Query: 194 VIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQ---- 253
           V+ V+Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+    ++     
Sbjct: 192 VVVVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPK 251

Query: 254 -----GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMT 313
                 V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   
Sbjct: 252 FPEAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFN 311

Query: 314 FYPITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP--GHQR 373
           F PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV + +P      
Sbjct: 312 FIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPN 371

Query: 374 KEPVCPSLQFSIMGQKLYVSIEQISIGRKPVMGMRLSLEGSKQNRLSIHLQHLASL-PKI 433
                P+L  + MG KLYV+   ++  + PV GMR  LEG K NRL+IHLQHL +    +
Sbjct: 372 MASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTV 431

Query: 434 LQPYWDTHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG--- 493
            +   D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S    
Sbjct: 432 GEKITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKS 491

Query: 494 ----VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSQK------- 553
                +IVTGAQL V   GS++VL+++L Y+++    + +  W H P   SQK       
Sbjct: 492 QNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSM 551

Query: 554 ---LVSSSVNSNNADDSSS-----------GSKSIQGNKLAKLVDMTEMCKGPQDPPGHW 586
              L S SV+ N      +           G      NK+ K VD++++C+GPQ  PGHW
Sbjct: 552 SMPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHW 611

BLAST of Lag0012272 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 198.7 bits (504), Expect = 1.3e-50
Identity = 127/335 (37.91%), Postives = 189/335 (56.42%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSS----VVEVDDKHTRDLRLYDDVVLLNVSRDIKN 73
           A+ ++G G+D+  D RL YCKG +  S    + E DD    ++ L   + + NVS+ IK 
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDD--NCEIVLPGGISIPNVSKSIK- 73

Query: 74  FQDQEPIGHRSSGICSFDEMVDYFNQRDGLSEGYSSGSFNSAFGFTGSKQTDSAATKSLS 133
             D+       S I  F +M + FNQ   L+    SG FN+ F F+   Q D+A TK+L+
Sbjct: 74  -CDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLA 133

Query: 134 TDGFYIPLAKFQLTNTPFVLQEHVKRAVPTSWDPPSLASFIENFGTHVITSITIGGKDVI 193
            DG +I L    L  +  +L+EHVK+AVP++WDP +LA FI+ +GTH+I S+ +GGKDVI
Sbjct: 134 FDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVI 193

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFN 253
           Y KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     +
Sbjct: 194 YAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTS 253

Query: 254 NQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITA 313
           + G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+
Sbjct: 254 SLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 313

Query: 314 LLDGVAGKEHLTRAILLYLEYKP--PLEELRYFLE 334
           LL+GV G   L+ AI LYL  KP   L  + +FLE
Sbjct: 314 LLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335