Lag0012217 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012217 vs. NCBI nr
Match: XP_022978183.1 (amino acid transporter ANT1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 379/418 (90.67%), Postives = 400/418 (95.69%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTD VEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV LAGV
Sbjct: 15  PTDAVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVALAGV 74

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLVKCR KL SQGRSKESRT+GDLGYICMG+KGRYLTEFLIF +QCGG+VAYL
Sbjct: 75  ATYYCMLLLVKCREKLTSQGRSKESRTFGDLGYICMGSKGRYLTEFLIFFAQCGGSVAYL 134

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHGLA+S YIFLIA VEVVLSWI SLAALAPFS+FADICNAIAMGIVV
Sbjct: 135 VFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIAMGIVV 194

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF++RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFP+V
Sbjct: 195 KEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPKV 254

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           LAQAFVGIT VYVLFGFSGYMAYGD+TRDIITLNLPNTWSTKA+QVGLCVGL+FTFPMML
Sbjct: 255 LAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFTFPMML 314

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVEDKL QS+WF KIQDS+NVFSGK++KVATYISRA IV+GLA LASFVPGFG+F
Sbjct: 315 HPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVPGFGLF 374

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGSTVCAL+SFVLPA FHL LMGSSLSFGQKVL SFILI GLLFAVYGTYN+ VG
Sbjct: 375 ASLVGSTVCALISFVLPAIFHLRLMGSSLSFGQKVLDSFILISGLLFAVYGTYNSLVG 432

BLAST of Lag0012217 vs. NCBI nr
Match: KAG6603501.1 (Amino acid transporter ANT1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033681.1 Amino acid transporter ANT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 733.8 bits (1893), Expect = 8.4e-208
Identity = 376/418 (89.95%), Postives = 397/418 (94.98%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTD VEIPLLHSS PRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV LAGV
Sbjct: 4   PTDAVEIPLLHSSPPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVALAGV 63

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLV CR KL SQGRSKESRT+GDLGYICMG+KGRYLTEFLIF +QCGG+VAYL
Sbjct: 64  ATYYCMLLLVNCREKLTSQGRSKESRTFGDLGYICMGSKGRYLTEFLIFFAQCGGSVAYL 123

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHGLA+S YIFLIA VEVVLSWI SLAALAPFS+FADICNAIAMGIVV
Sbjct: 124 VFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIAMGIVV 183

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF++RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFP+V
Sbjct: 184 KEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPKV 243

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           LAQAFVGIT VYVLFGFSGYMAYGD+TRDIITLNLPNTWSTKA+QVGLCVGL+FTFPMML
Sbjct: 244 LAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFTFPMML 303

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVEDKL QS+WF KIQDS+NVFSGK++KVATYISRA IV+GLA LASFVPGFG+F
Sbjct: 304 HPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVPGFGLF 363

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGSTVCAL+SFVLPA FHL LMGSSLSF QKVL SFILI GLLFAVYGTYN+ VG
Sbjct: 364 ASLVGSTVCALISFVLPAIFHLRLMGSSLSFSQKVLDSFILISGLLFAVYGTYNSLVG 421

BLAST of Lag0012217 vs. NCBI nr
Match: XP_022950929.1 (amino acid transporter ANT1 [Cucurbita moschata])

HSP 1 Score: 733.8 bits (1893), Expect = 8.4e-208
Identity = 376/418 (89.95%), Postives = 397/418 (94.98%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTD VEIPLLHSS PRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV LAGV
Sbjct: 15  PTDAVEIPLLHSSPPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVALAGV 74

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLVKCR KL SQGRS ESRT+GDLGYICMG+KGRYLTEFLIF +QCGG+VAYL
Sbjct: 75  ATYYCMLLLVKCREKLTSQGRSNESRTFGDLGYICMGSKGRYLTEFLIFFAQCGGSVAYL 134

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHGLA+S YIFLIA VEVVLSWI SLAALAPFS+FADICNAIAMGIVV
Sbjct: 135 VFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIAMGIVV 194

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF++RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFP+V
Sbjct: 195 KEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPKV 254

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           LAQAFVGIT VYVLFGFSGYMAYGD+TRDIITLNLPNTWSTKA+QVGLCVGL+FTFPMML
Sbjct: 255 LAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFTFPMML 314

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVEDKL QS+WF KIQDS+NVFSGK++KVATYISRA IV+GLA LASFVPGFG+F
Sbjct: 315 HPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVPGFGLF 374

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGSTVCAL+SFVLPA FHL LMGSSLSF QKVL SFILI GLLFAVYGTYN+ VG
Sbjct: 375 ASLVGSTVCALISFVLPAIFHLRLMGSSLSFSQKVLDSFILISGLLFAVYGTYNSLVG 432

BLAST of Lag0012217 vs. NCBI nr
Match: XP_023543142.1 (amino acid transporter ANT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 726.9 bits (1875), Expect = 1.0e-205
Identity = 375/418 (89.71%), Postives = 396/418 (94.74%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTD VEIPLLHSSS RTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV LAGV
Sbjct: 4   PTDAVEIPLLHSSSLRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVALAGV 63

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLVKCR KL SQGRS ESRT+GDLGYICMG+KGRYLTEFLIF +QCGG+VAYL
Sbjct: 64  ATYYCMLLLVKCREKLTSQGRSNESRTFGDLGYICMGSKGRYLTEFLIFFAQCGGSVAYL 123

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHGLA+S YIFLIA VEVVLSWI SLAALAPFS+FADICNAIAMGIVV
Sbjct: 124 VFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIAMGIVV 183

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF++RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFP+V
Sbjct: 184 KEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPKV 243

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           LAQAFVGIT VYVLFGFSGYMAYGD+TRDIITLNLPNTWSTKAVQVGLCVGL+FTFPMML
Sbjct: 244 LAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAVQVGLCVGLVFTFPMML 303

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVEDKL QS+ F KIQDS+NVFSGK++KVATYISRA IV+GLA LASFVPGFG+F
Sbjct: 304 HPINEIVEDKLKQSSLFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAILASFVPGFGLF 363

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGSTVCAL+SFVLPA FHL LMGSSLSF QKVL SFILI GLLFA+YGTYN+ VG
Sbjct: 364 ASLVGSTVCALISFVLPAIFHLRLMGSSLSFSQKVLDSFILISGLLFAIYGTYNSLVG 421

BLAST of Lag0012217 vs. NCBI nr
Match: XP_022156208.1 (amino acid transporter ANT1, partial [Momordica charantia])

HSP 1 Score: 704.1 bits (1816), Expect = 7.1e-199
Identity = 363/420 (86.43%), Postives = 383/420 (91.19%), Query Frame = 0

Query: 9   VEIPLLHSSSPR-----TTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAG 68
           V IPLL SSS       TTSTLQ LGNIIVSV+GTGILGLPYAFRIAGW AGS GV+L G
Sbjct: 1   VGIPLLDSSSSSSSSQPTTSTLQTLGNIIVSVIGTGILGLPYAFRIAGWAAGSLGVILTG 60

Query: 69  VATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAY 128
           VATYYCMLLLV CR KLASQGRSKESRTYGDLGY+CMGNKGRYLTEFLIF +QCGG+VAY
Sbjct: 61  VATYYCMLLLVNCREKLASQGRSKESRTYGDLGYMCMGNKGRYLTEFLIFFAQCGGSVAY 120

Query: 129 LVFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIV 188
           LVFIGQNLSSVFQGHGLA SSYIFLIAAVE+V+SWIGSLAALAPFS+FADICNAIAMGIV
Sbjct: 121 LVFIGQNLSSVFQGHGLAFSSYIFLIAAVEIVMSWIGSLAALAPFSIFADICNAIAMGIV 180

Query: 189 VKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPR 248
           VKEDIQKAIAGGISF ER  ITSNLRGLPFAGGMAVFCFEGFGMTLALESSMK KA FPR
Sbjct: 181 VKEDIQKAIAGGISFGERMVITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKYKATFPR 240

Query: 249 VLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMM 308
           VLAQAFVGITI+YVLFGFSGYMAYGD+TRDIITLNLPNTWST+AVQVGLCVGL+FTFP+M
Sbjct: 241 VLAQAFVGITIMYVLFGFSGYMAYGDETRDIITLNLPNTWSTRAVQVGLCVGLVFTFPIM 300

Query: 309 LHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGV 368
           LHPINEIVE KL QSNW  K+QDSDNVFS K + +  YISRA IV+GLATLASF+PGFGV
Sbjct: 301 LHPINEIVEGKLKQSNWLQKVQDSDNVFSRKGVTLVAYISRAIIVLGLATLASFIPGFGV 360

Query: 369 FASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVGF 424
           FAS VGSTVCAL+SFVLPA FHL+LMGSSLSFGQKVL SFILICGL FAVYGTYNT VGF
Sbjct: 361 FASFVGSTVCALISFVLPAIFHLLLMGSSLSFGQKVLDSFILICGLFFAVYGTYNTIVGF 420

BLAST of Lag0012217 vs. ExPASy Swiss-Prot
Match: Q9SF09 (Amino acid transporter ANT1 OS=Arabidopsis thaliana OX=3702 GN=ANT1 PE=1 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 2.6e-151
Identity = 268/422 (63.51%), Postives = 332/422 (78.67%), Query Frame = 0

Query: 6   TDTVEIPLLHSSSPRT-----TSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVV 65
           T    +PL+ S    T     TS LQ LGNIIVS+VGTG+LGLPYAFRIAGW AGS GV+
Sbjct: 10  TGDSSLPLIKSPPSETTGGDRTSALQTLGNIIVSIVGTGVLGLPYAFRIAGWLAGSLGVI 69

Query: 66  LAGVATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGA 125
           + G ATYYCMLLL++CR KL S+   +ES+TYGDLG+ CMG KGRYLTEFLIF +QCGG+
Sbjct: 70  IVGFATYYCMLLLIQCRDKLESEEGEEESKTYGDLGFKCMGTKGRYLTEFLIFTAQCGGS 129

Query: 126 VAYLVFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAM 185
           VAYLVFIG+NLSS+F  +GL++ S+I ++  +EV LSWI SL+AL+PFS+FADICN IAM
Sbjct: 130 VAYLVFIGRNLSSIFSSYGLSMVSFILILVPIEVGLSWITSLSALSPFSIFADICNIIAM 189

Query: 186 GIVVKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAA 245
             VVKE+++  I G  SF +RTAI+S + GLPFAGG+AVFCFEGF MTLALESSM+++ A
Sbjct: 190 CFVVKENVEMVIEGDFSFSDRTAISSTIGGLPFAGGVAVFCFEGFAMTLALESSMREREA 249

Query: 246 FPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTF 305
           FP++LA+   GIT VYVLFGF GYMAYGD T+DIITLNLPN WS  AVQ+GLCVGL FTF
Sbjct: 250 FPKLLAKVLAGITFVYVLFGFCGYMAYGDQTKDIITLNLPNNWSAIAVQIGLCVGLTFTF 309

Query: 306 PMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPG 365
           P+M+HP+NEI+E KL + +W  K  +  +  +G   K A + +R  +V+GLA +AS VPG
Sbjct: 310 PIMVHPLNEIIEQKLKRIDWLQKHHNGYSNETGSVSKFAIFTTRTLLVVGLAAIASLVPG 369

Query: 366 FGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTF 423
           FG FASLVGST+CAL+SFVLPA++HL L+G SL+   K +  FI+ICGL+FAVYGTYNT 
Sbjct: 370 FGTFASLVGSTLCALISFVLPASYHLTLLGPSLNVWNKSIDVFIVICGLIFAVYGTYNTI 429

BLAST of Lag0012217 vs. ExPASy Swiss-Prot
Match: Q9SVG0 (Amino acid transporter AVT3C OS=Arabidopsis thaliana OX=3702 GN=AVT3C PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.2e-61
Identity = 159/437 (36.38%), Postives = 238/437 (54.46%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           P    + PLL    P  +S  +   N+ ++VVG G+LGLPYAF+  GW  G   +V   V
Sbjct: 18  PPAREDSPLL-GKGPPLSSQFKTFANVFIAVVGAGVLGLPYAFKRTGWLMGVLLLVSVSV 77

Query: 65  ATYYCMLLLVKCRGKLAS--QGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVA 124
            T++CM+LLV  R KL S   G SK   ++GDLG+   G+ GR + +  I +SQ G  V 
Sbjct: 78  LTHHCMMLLVYTRRKLDSFNAGISKIG-SFGDLGFAVCGSLGRIVVDLFIILSQAGFCVG 137

Query: 125 YLVFIGQNLSSVFQGHG---------------LALSS---YIFLIAAVEVVLSWIGSLAA 184
           YL+FIG  L+++                    L +SS   YI+     ++ L+ I +L  
Sbjct: 138 YLIFIGTTLANLSDPESPTSLRHQFTRLGSEFLGVSSKSLYIWGCFPFQLGLNSIKTLTH 197

Query: 185 LAPFSMFADICNAIAMGIVVKEDIQKAIAGGISFEERTAITS--NLRGLPFAGGMAVFCF 244
           LAP S+FADI +  AM +V+ ED        I  ++R  + +   +    +  G+AV+ F
Sbjct: 198 LAPLSIFADIVDLGAMAVVIVEDSM------IILKQRPDVVAFGGMSLFLYGMGVAVYSF 257

Query: 245 EGFGMTLALESSMKDKAAFPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNT 304
           EG GM L LES MKDK  F +VLA     I+++Y+ FG  GY+A+G+DT DIIT NL   
Sbjct: 258 EGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGILGYLAFGEDTMDIITANLGAG 317

Query: 305 WSTKAVQVGLCVGLLFTFPMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYI 364
             +  VQ+GLC+ L FTFP+M++P+ EIVE + ++  +   +                  
Sbjct: 318 LVSTVVQLGLCINLFFTFPLMMNPVFEIVERRFSRGMYSAWL------------------ 377

Query: 365 SRATIVIGLATLASFVPGFGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSS 420
            R  +V+ +  +A FVP F  F SLVGS+ C +L FVLPA FHL++    + + Q    +
Sbjct: 378 -RWVLVLAVTLVALFVPNFADFLSLVGSSTCCVLGFVLPALFHLLVFKEEMGWLQWSSDT 427

BLAST of Lag0012217 vs. ExPASy Swiss-Prot
Match: F4ILY9 (Amino acid transporter AVT3B OS=Arabidopsis thaliana OX=3702 GN=AVT3B PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.2e-61
Identity = 156/434 (35.94%), Postives = 235/434 (54.15%), Query Frame = 0

Query: 1   MEQQPTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVV 60
           +E+Q     + PLL    P  +S  +   N+ +++VG G+LGLPYAF+  GW  G   + 
Sbjct: 3   LEEQGRAREDTPLLGKGRP-LSSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLF 62

Query: 61  LAGVATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGA 120
                  +CM+LLV  R KL          ++GDLG+   GN GR++ + LI +SQ G  
Sbjct: 63  SVAALINHCMMLLVHIRRKLGVSNIG----SFGDLGFAACGNLGRFVVDILIILSQAGFC 122

Query: 121 VAYLVFIGQNLSSVFQ----------GHGLALSS---YIFLIAAVEVVLSWIGSLAALAP 180
           V YL+FIG  L+++ +           H + +S    YI+     ++ L+ I +L  LAP
Sbjct: 123 VGYLIFIGNTLANLSKPTKSTTLMSLRHLMGVSPKSLYIWGCFPFQLGLNSIKTLTHLAP 182

Query: 181 FSMFADICNAIAMGIVVKEDIQKAIAGGISFEERTAITS--NLRGLPFAGGMAVFCFEGF 240
            S+FAD+ +  AM +V+ EDI+      I+  +R  + +   +    +  G+AV+ FEG 
Sbjct: 183 LSIFADVVDLGAMAVVIVEDIK------ITVVQRPQVVAFGGMSVFFYGMGVAVYAFEGV 242

Query: 241 GMTLALESSMKDKAAFPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWST 300
           GM L LES  KDK  F +VLA + + I ++Y  FG  GYMA+GDDT DIIT NL     +
Sbjct: 243 GMVLPLESETKDKDKFGKVLALSMLFIAVMYGSFGVLGYMAFGDDTMDIITANLGAGVVS 302

Query: 301 KAVQVGLCVGLLFTFPMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRA 360
             VQ+GLC+ L FTFP+M++P+ EIVE +     W G               +     R 
Sbjct: 303 SLVQLGLCINLFFTFPLMMNPVFEIVERRF----WSG---------------MYCVWLRW 362

Query: 361 TIVIGLATLASFVPGFGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFIL 420
            +V+ +  +A  VP F  F SLVGS+VC  L FVLP+ FHL++    + + Q+ L   IL
Sbjct: 363 LLVLAVTLVALLVPNFADFLSLVGSSVCCALGFVLPSLFHLMVFKDEMEWKQRALDVGIL 406

BLAST of Lag0012217 vs. ExPASy Swiss-Prot
Match: Q9FKY3 (Amino acid transporter AVT3A OS=Arabidopsis thaliana OX=3702 GN=AVT3A PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.1e-56
Identity = 150/417 (35.97%), Postives = 224/417 (53.72%), Query Frame = 0

Query: 8   TVEIPLLHSSSPRTTSTL-QALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGVAT 67
           T + PLL    PRT S+  +   N+ +++VG G+LGLPY F+  GW  G   ++     T
Sbjct: 26  TEDTPLL---GPRTLSSQPKTFANVFIAIVGAGVLGLPYTFKKTGWLLGLLTLLFVSSLT 85

Query: 68  YYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYLVF 127
           ++CM+LLV  R KL S        ++GDLG    G  GR + + ++ +SQ G  V+YL+F
Sbjct: 86  FFCMMLLVHTRRKLESLSGFNSITSFGDLGESVCGPAGRLVVDVMLVLSQSGFCVSYLIF 145

Query: 128 IG---QNLSSVFQGHGLAL---SSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAM 187
           +     NL S    H L L   S Y++     ++ L+ I SL  LAP S+FADI +  A 
Sbjct: 146 VATTMANLLSRGTEHILGLDAASIYLWGCFPFQLGLNSIPSLTHLAPLSIFADIVDVAAT 205

Query: 188 GIVVKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAA 247
            +V+ +D+   +           ++    GL    G+AV+ FEG GM L LE   K K  
Sbjct: 206 LVVMVQDVFIFLKRRPPLRVFGGVSVFFYGL----GVAVYAFEGIGMVLPLELEAKYKDK 265

Query: 248 FPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTF 307
           F R L  A   I+I+Y  FG  GYMAYG++T+DIIT NL     +  VQ+GL + L FTF
Sbjct: 266 FGRALGLAMGLISIMYGAFGLLGYMAYGEETKDIITTNLGTGVVSTLVQLGLAINLFFTF 325

Query: 308 PMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPG 367
           P+M+ P+ E+VE +L  S +                  + ++  AT+++ +  +A  VP 
Sbjct: 326 PLMMQPVYEVVERRLCSSRY------------------SVWVRWATVLV-VTLVALLVPN 385

Query: 368 FGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTY 418
           F  F SLVGS+VC +L FVLP+ FHL    + LS  + V+   + + G++ A+ GT+
Sbjct: 386 FADFLSLVGSSVCVVLGFVLPSLFHLQAFKNELSITRIVVDVLVFLIGVMIAITGTW 416

BLAST of Lag0012217 vs. ExPASy Swiss-Prot
Match: Q495N2 (Proton-coupled amino acid transporter 3 OS=Homo sapiens OX=9606 GN=SLC36A3 PE=2 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 9.8e-34
Identity = 125/434 (28.80%), Postives = 201/434 (46.31%), Query Frame = 0

Query: 14  LHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGVATYYCMLLL 73
           +H +     S +Q L +++   +GTG+LGLP A + AG   G   ++  GV T +CM++L
Sbjct: 34  VHPAGEAGLSMMQTLIHLLKCNIGTGLLGLPLAIKNAGLLVGPVSLLAIGVLTVHCMVIL 93

Query: 74  VKCRGKLASQGRSKESRTYGD---LGYICMGNK--------GRYLTEFLIFISQCGGAVA 133
           + C   L SQ   K    YG+    G     N         GRY   FL+ I+Q G    
Sbjct: 94  LNCAQHL-SQRLQKTFVNYGEATMYGLETCPNTWLRAHAVWGRYTVSFLLVITQLGFCSV 153

Query: 134 YLVFIGQNL----------SSVFQGHG-------LALSSYIFLIAAVEVVLSWIGSLAAL 193
           Y +F+  NL          S++ Q          L +  Y+ +I    ++L +I +L  L
Sbjct: 154 YFMFMADNLQQMVEKAHVTSNICQPREILTLTPILDIRFYMLIILPFLILLVFIQNLKVL 213

Query: 194 APFSMFADICNAIAMGIVVKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGF 253
           + FS  A+I    +M ++      + I  GI +     + +N +      G A+F FEG 
Sbjct: 214 SVFSTLANITTLGSMALIF-----EYIMEGIPYPSNLPLMANWKTFLLFFGTAIFTFEGV 273

Query: 254 GMTLALESSMKDKAAFPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWST 313
           GM L L++ MK    F  VL      + I+Y+L G  GYM +G DT+  ITLNLPN W  
Sbjct: 274 GMVLPLKNQMKHPQQFSFVLYLGMSIVIILYILLGTLGYMKFGSDTQASITLNLPNCWLY 333

Query: 314 KAVQVGLCVGLLFTFPMMLH-PINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISR 373
           ++V++   +G+ FT+ +  H P   I+   ++Q      + +S  +F    +       R
Sbjct: 334 QSVKLMYSIGIFFTYALQFHVPAEIIIPFAISQ------VSESWALFVDLSV-------R 393

Query: 374 ATIVIGLATLASFVPGFGVFASLVGSTVCALLSFVLPATFHLILMGS-SLSFGQKVLSSF 418
           + +V      A  +P   +  SLVGS   + L+ ++PA   +++  S  +S         
Sbjct: 394 SALVCLTCVSAILIPRLDLVISLVGSVSSSALALIIPALLEIVIFYSEDMSCVTIAKDIM 448

BLAST of Lag0012217 vs. ExPASy TrEMBL
Match: A0A6J1ISA2 (amino acid transporter ANT1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478243 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 5.7e-210
Identity = 379/418 (90.67%), Postives = 400/418 (95.69%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTD VEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV LAGV
Sbjct: 15  PTDAVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVALAGV 74

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLVKCR KL SQGRSKESRT+GDLGYICMG+KGRYLTEFLIF +QCGG+VAYL
Sbjct: 75  ATYYCMLLLVKCREKLTSQGRSKESRTFGDLGYICMGSKGRYLTEFLIFFAQCGGSVAYL 134

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHGLA+S YIFLIA VEVVLSWI SLAALAPFS+FADICNAIAMGIVV
Sbjct: 135 VFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIAMGIVV 194

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF++RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFP+V
Sbjct: 195 KEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPKV 254

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           LAQAFVGIT VYVLFGFSGYMAYGD+TRDIITLNLPNTWSTKA+QVGLCVGL+FTFPMML
Sbjct: 255 LAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFTFPMML 314

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVEDKL QS+WF KIQDS+NVFSGK++KVATYISRA IV+GLA LASFVPGFG+F
Sbjct: 315 HPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVPGFGLF 374

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGSTVCAL+SFVLPA FHL LMGSSLSFGQKVL SFILI GLLFAVYGTYN+ VG
Sbjct: 375 ASLVGSTVCALISFVLPAIFHLRLMGSSLSFGQKVLDSFILISGLLFAVYGTYNSLVG 432

BLAST of Lag0012217 vs. ExPASy TrEMBL
Match: A0A6J1GH88 (amino acid transporter ANT1 OS=Cucurbita moschata OX=3662 GN=LOC111453876 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 4.1e-208
Identity = 376/418 (89.95%), Postives = 397/418 (94.98%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTD VEIPLLHSS PRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV LAGV
Sbjct: 15  PTDAVEIPLLHSSPPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVALAGV 74

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLVKCR KL SQGRS ESRT+GDLGYICMG+KGRYLTEFLIF +QCGG+VAYL
Sbjct: 75  ATYYCMLLLVKCREKLTSQGRSNESRTFGDLGYICMGSKGRYLTEFLIFFAQCGGSVAYL 134

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHGLA+S YIFLIA VEVVLSWI SLAALAPFS+FADICNAIAMGIVV
Sbjct: 135 VFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIAMGIVV 194

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF++RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFP+V
Sbjct: 195 KEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPKV 254

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           LAQAFVGIT VYVLFGFSGYMAYGD+TRDIITLNLPNTWSTKA+QVGLCVGL+FTFPMML
Sbjct: 255 LAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFTFPMML 314

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVEDKL QS+WF KIQDS+NVFSGK++KVATYISRA IV+GLA LASFVPGFG+F
Sbjct: 315 HPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVPGFGLF 374

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGSTVCAL+SFVLPA FHL LMGSSLSF QKVL SFILI GLLFAVYGTYN+ VG
Sbjct: 375 ASLVGSTVCALISFVLPAIFHLRLMGSSLSFSQKVLDSFILISGLLFAVYGTYNSLVG 432

BLAST of Lag0012217 vs. ExPASy TrEMBL
Match: A0A6J1DPZ9 (amino acid transporter ANT1 OS=Momordica charantia OX=3673 GN=LOC111023151 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 3.5e-199
Identity = 363/420 (86.43%), Postives = 383/420 (91.19%), Query Frame = 0

Query: 9   VEIPLLHSSSPR-----TTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAG 68
           V IPLL SSS       TTSTLQ LGNIIVSV+GTGILGLPYAFRIAGW AGS GV+L G
Sbjct: 1   VGIPLLDSSSSSSSSQPTTSTLQTLGNIIVSVIGTGILGLPYAFRIAGWAAGSLGVILTG 60

Query: 69  VATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAY 128
           VATYYCMLLLV CR KLASQGRSKESRTYGDLGY+CMGNKGRYLTEFLIF +QCGG+VAY
Sbjct: 61  VATYYCMLLLVNCREKLASQGRSKESRTYGDLGYMCMGNKGRYLTEFLIFFAQCGGSVAY 120

Query: 129 LVFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIV 188
           LVFIGQNLSSVFQGHGLA SSYIFLIAAVE+V+SWIGSLAALAPFS+FADICNAIAMGIV
Sbjct: 121 LVFIGQNLSSVFQGHGLAFSSYIFLIAAVEIVMSWIGSLAALAPFSIFADICNAIAMGIV 180

Query: 189 VKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPR 248
           VKEDIQKAIAGGISF ER  ITSNLRGLPFAGGMAVFCFEGFGMTLALESSMK KA FPR
Sbjct: 181 VKEDIQKAIAGGISFGERMVITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKYKATFPR 240

Query: 249 VLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMM 308
           VLAQAFVGITI+YVLFGFSGYMAYGD+TRDIITLNLPNTWST+AVQVGLCVGL+FTFP+M
Sbjct: 241 VLAQAFVGITIMYVLFGFSGYMAYGDETRDIITLNLPNTWSTRAVQVGLCVGLVFTFPIM 300

Query: 309 LHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGV 368
           LHPINEIVE KL QSNW  K+QDSDNVFS K + +  YISRA IV+GLATLASF+PGFGV
Sbjct: 301 LHPINEIVEGKLKQSNWLQKVQDSDNVFSRKGVTLVAYISRAIIVLGLATLASFIPGFGV 360

Query: 369 FASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVGF 424
           FAS VGSTVCAL+SFVLPA FHL+LMGSSLSFGQKVL SFILICGL FAVYGTYNT VGF
Sbjct: 361 FASFVGSTVCALISFVLPAIFHLLLMGSSLSFGQKVLDSFILICGLFFAVYGTYNTIVGF 420

BLAST of Lag0012217 vs. ExPASy TrEMBL
Match: A0A1S3B5C4 (amino acid transporter ANT1 OS=Cucumis melo OX=3656 GN=LOC103485964 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.6e-196
Identity = 357/418 (85.41%), Postives = 385/418 (92.11%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           PTDT+EIPLL+SSS RTTST QAL NIIVSVVGTG+LGLP+AFRIAG+ AGSFGV+L  +
Sbjct: 4   PTDTMEIPLLNSSS-RTTSTFQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLVAL 63

Query: 65  ATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYL 124
           ATYYCMLLLVKCR KL  QGRS ES+TYGDLGYICMGNKGRYLTEFLIF +QCGG+VAYL
Sbjct: 64  ATYYCMLLLVKCREKLTLQGRSNESKTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYL 123

Query: 125 VFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVV 184
           VFIGQNLSSVFQGHG+ LSSYIFLIAAVEVVLSWIGSLAALAPFS+FADICNAIAMGIVV
Sbjct: 124 VFIGQNLSSVFQGHGIPLSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVV 183

Query: 185 KEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRV 244
           KEDIQKAIAGGISF ERTAITSNLRGLPFAGGMAVFCFEGFGMTLAL+SSMKDKAAFP+V
Sbjct: 184 KEDIQKAIAGGISFNERTAITSNLRGLPFAGGMAVFCFEGFGMTLALQSSMKDKAAFPKV 243

Query: 245 LAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMML 304
           L QA VGITIVY+LFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGL+FTFP+ML
Sbjct: 244 LGQALVGITIVYILFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLIFTFPIML 303

Query: 305 HPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVF 364
           HPINEIVE KL QSNWF KI+D+D +F GKR + ATY+SRA IV+ LA LASFVPGFGVF
Sbjct: 304 HPINEIVEGKLAQSNWFEKIEDNDVIFLGKRAQAATYMSRAVIVLVLAILASFVPGFGVF 363

Query: 365 ASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           ASLVGST+CAL+SFVLPA FHL+LMGSSL   QKVL S ILICGL+FAVYGTYN+  G
Sbjct: 364 ASLVGSTICALISFVLPAIFHLMLMGSSLCLSQKVLDSSILICGLIFAVYGTYNSLFG 420

BLAST of Lag0012217 vs. ExPASy TrEMBL
Match: A0A0A0L026 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G193760 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.3e-194
Identity = 354/416 (85.10%), Postives = 383/416 (92.07%), Query Frame = 0

Query: 9   VEIPLLHSSSPR--TTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGVAT 68
           ++IPLL+SSS    TTST QAL NIIVSVVGTG+LGLP+AFRIAG+ AGSFGV+L  +AT
Sbjct: 1   MDIPLLNSSSSSSPTTSTFQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLVALAT 60

Query: 69  YYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYLVF 128
           YYCMLLLVKCR KLA QGRSKES+TYGDLGYICMGNKGRYLTEFLIF +QCGG+VAYLVF
Sbjct: 61  YYCMLLLVKCREKLALQGRSKESQTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF 120

Query: 129 IGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAMGIVVKE 188
           IGQNLSSVFQ +G+ LSSYIFLIAAVEVVLSWIGSLAALAPFS+FADICNAIAMGIVVKE
Sbjct: 121 IGQNLSSVFQSYGIPLSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE 180

Query: 189 DIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRVLA 248
           DIQKAIAGGISF ERTAITSNLRGLPFAGGMAVFCFEGFGMTLAL+SSMKDKAAFP+VL 
Sbjct: 181 DIQKAIAGGISFNERTAITSNLRGLPFAGGMAVFCFEGFGMTLALQSSMKDKAAFPKVLG 240

Query: 249 QAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTFPMMLHP 308
           QA VGITIVY+LFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGL+FTFP+MLHP
Sbjct: 241 QALVGITIVYILFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLVFTFPIMLHP 300

Query: 309 INEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVFAS 368
           INEIVE KL QSNWF KI+D+D++FSGKR KVATYISR  IV+ LA LASFVPGFGVFAS
Sbjct: 301 INEIVEGKLAQSNWFEKIEDNDDIFSGKRAKVATYISRTLIVLVLAILASFVPGFGVFAS 360

Query: 369 LVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTFVG 423
           LVGST+CAL+SFVLPA FHL+LMGSSL   QKVL S ILICGL+FA YGTYN+ VG
Sbjct: 361 LVGSTICALISFVLPAIFHLMLMGSSLCLSQKVLDSSILICGLIFAAYGTYNSLVG 416

BLAST of Lag0012217 vs. TAIR 10
Match: AT3G11900.1 (aromatic and neutral transporter 1 )

HSP 1 Score: 536.6 bits (1381), Expect = 1.8e-152
Identity = 268/422 (63.51%), Postives = 332/422 (78.67%), Query Frame = 0

Query: 6   TDTVEIPLLHSSSPRT-----TSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVV 65
           T    +PL+ S    T     TS LQ LGNIIVS+VGTG+LGLPYAFRIAGW AGS GV+
Sbjct: 10  TGDSSLPLIKSPPSETTGGDRTSALQTLGNIIVSIVGTGVLGLPYAFRIAGWLAGSLGVI 69

Query: 66  LAGVATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGA 125
           + G ATYYCMLLL++CR KL S+   +ES+TYGDLG+ CMG KGRYLTEFLIF +QCGG+
Sbjct: 70  IVGFATYYCMLLLIQCRDKLESEEGEEESKTYGDLGFKCMGTKGRYLTEFLIFTAQCGGS 129

Query: 126 VAYLVFIGQNLSSVFQGHGLALSSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAM 185
           VAYLVFIG+NLSS+F  +GL++ S+I ++  +EV LSWI SL+AL+PFS+FADICN IAM
Sbjct: 130 VAYLVFIGRNLSSIFSSYGLSMVSFILILVPIEVGLSWITSLSALSPFSIFADICNIIAM 189

Query: 186 GIVVKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAA 245
             VVKE+++  I G  SF +RTAI+S + GLPFAGG+AVFCFEGF MTLALESSM+++ A
Sbjct: 190 CFVVKENVEMVIEGDFSFSDRTAISSTIGGLPFAGGVAVFCFEGFAMTLALESSMREREA 249

Query: 246 FPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTF 305
           FP++LA+   GIT VYVLFGF GYMAYGD T+DIITLNLPN WS  AVQ+GLCVGL FTF
Sbjct: 250 FPKLLAKVLAGITFVYVLFGFCGYMAYGDQTKDIITLNLPNNWSAIAVQIGLCVGLTFTF 309

Query: 306 PMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPG 365
           P+M+HP+NEI+E KL + +W  K  +  +  +G   K A + +R  +V+GLA +AS VPG
Sbjct: 310 PIMVHPLNEIIEQKLKRIDWLQKHHNGYSNETGSVSKFAIFTTRTLLVVGLAAIASLVPG 369

Query: 366 FGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNTF 423
           FG FASLVGST+CAL+SFVLPA++HL L+G SL+   K +  FI+ICGL+FAVYGTYNT 
Sbjct: 370 FGTFASLVGSTLCALISFVLPASYHLTLLGPSLNVWNKSIDVFIVICGLIFAVYGTYNTI 429

BLAST of Lag0012217 vs. TAIR 10
Match: AT4G38250.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.3e-62
Identity = 159/437 (36.38%), Postives = 238/437 (54.46%), Query Frame = 0

Query: 5   PTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGV 64
           P    + PLL    P  +S  +   N+ ++VVG G+LGLPYAF+  GW  G   +V   V
Sbjct: 18  PPAREDSPLL-GKGPPLSSQFKTFANVFIAVVGAGVLGLPYAFKRTGWLMGVLLLVSVSV 77

Query: 65  ATYYCMLLLVKCRGKLAS--QGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVA 124
            T++CM+LLV  R KL S   G SK   ++GDLG+   G+ GR + +  I +SQ G  V 
Sbjct: 78  LTHHCMMLLVYTRRKLDSFNAGISKIG-SFGDLGFAVCGSLGRIVVDLFIILSQAGFCVG 137

Query: 125 YLVFIGQNLSSVFQGHG---------------LALSS---YIFLIAAVEVVLSWIGSLAA 184
           YL+FIG  L+++                    L +SS   YI+     ++ L+ I +L  
Sbjct: 138 YLIFIGTTLANLSDPESPTSLRHQFTRLGSEFLGVSSKSLYIWGCFPFQLGLNSIKTLTH 197

Query: 185 LAPFSMFADICNAIAMGIVVKEDIQKAIAGGISFEERTAITS--NLRGLPFAGGMAVFCF 244
           LAP S+FADI +  AM +V+ ED        I  ++R  + +   +    +  G+AV+ F
Sbjct: 198 LAPLSIFADIVDLGAMAVVIVEDSM------IILKQRPDVVAFGGMSLFLYGMGVAVYSF 257

Query: 245 EGFGMTLALESSMKDKAAFPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNT 304
           EG GM L LES MKDK  F +VLA     I+++Y+ FG  GY+A+G+DT DIIT NL   
Sbjct: 258 EGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGILGYLAFGEDTMDIITANLGAG 317

Query: 305 WSTKAVQVGLCVGLLFTFPMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYI 364
             +  VQ+GLC+ L FTFP+M++P+ EIVE + ++  +   +                  
Sbjct: 318 LVSTVVQLGLCINLFFTFPLMMNPVFEIVERRFSRGMYSAWL------------------ 377

Query: 365 SRATIVIGLATLASFVPGFGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSS 420
            R  +V+ +  +A FVP F  F SLVGS+ C +L FVLPA FHL++    + + Q    +
Sbjct: 378 -RWVLVLAVTLVALFVPNFADFLSLVGSSTCCVLGFVLPALFHLLVFKEEMGWLQWSSDT 427

BLAST of Lag0012217 vs. TAIR 10
Match: AT2G42005.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 236.9 bits (603), Expect = 3.0e-62
Identity = 156/434 (35.94%), Postives = 235/434 (54.15%), Query Frame = 0

Query: 1   MEQQPTDTVEIPLLHSSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVV 60
           +E+Q     + PLL    P  +S  +   N+ +++VG G+LGLPYAF+  GW  G   + 
Sbjct: 3   LEEQGRAREDTPLLGKGRP-LSSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLF 62

Query: 61  LAGVATYYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGA 120
                  +CM+LLV  R KL          ++GDLG+   GN GR++ + LI +SQ G  
Sbjct: 63  SVAALINHCMMLLVHIRRKLGVSNIG----SFGDLGFAACGNLGRFVVDILIILSQAGFC 122

Query: 121 VAYLVFIGQNLSSVFQ----------GHGLALSS---YIFLIAAVEVVLSWIGSLAALAP 180
           V YL+FIG  L+++ +           H + +S    YI+     ++ L+ I +L  LAP
Sbjct: 123 VGYLIFIGNTLANLSKPTKSTTLMSLRHLMGVSPKSLYIWGCFPFQLGLNSIKTLTHLAP 182

Query: 181 FSMFADICNAIAMGIVVKEDIQKAIAGGISFEERTAITS--NLRGLPFAGGMAVFCFEGF 240
            S+FAD+ +  AM +V+ EDI+      I+  +R  + +   +    +  G+AV+ FEG 
Sbjct: 183 LSIFADVVDLGAMAVVIVEDIK------ITVVQRPQVVAFGGMSVFFYGMGVAVYAFEGV 242

Query: 241 GMTLALESSMKDKAAFPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWST 300
           GM L LES  KDK  F +VLA + + I ++Y  FG  GYMA+GDDT DIIT NL     +
Sbjct: 243 GMVLPLESETKDKDKFGKVLALSMLFIAVMYGSFGVLGYMAFGDDTMDIITANLGAGVVS 302

Query: 301 KAVQVGLCVGLLFTFPMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRA 360
             VQ+GLC+ L FTFP+M++P+ EIVE +     W G               +     R 
Sbjct: 303 SLVQLGLCINLFFTFPLMMNPVFEIVERRF----WSG---------------MYCVWLRW 362

Query: 361 TIVIGLATLASFVPGFGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFIL 420
            +V+ +  +A  VP F  F SLVGS+VC  L FVLP+ FHL++    + + Q+ L   IL
Sbjct: 363 LLVLAVTLVALLVPNFADFLSLVGSSVCCALGFVLPSLFHLMVFKDEMEWKQRALDVGIL 406

BLAST of Lag0012217 vs. TAIR 10
Match: AT5G65990.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-57
Identity = 150/417 (35.97%), Postives = 224/417 (53.72%), Query Frame = 0

Query: 8   TVEIPLLHSSSPRTTSTL-QALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGVAT 67
           T + PLL    PRT S+  +   N+ +++VG G+LGLPY F+  GW  G   ++     T
Sbjct: 26  TEDTPLL---GPRTLSSQPKTFANVFIAIVGAGVLGLPYTFKKTGWLLGLLTLLFVSSLT 85

Query: 68  YYCMLLLVKCRGKLASQGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYLVF 127
           ++CM+LLV  R KL S        ++GDLG    G  GR + + ++ +SQ G  V+YL+F
Sbjct: 86  FFCMMLLVHTRRKLESLSGFNSITSFGDLGESVCGPAGRLVVDVMLVLSQSGFCVSYLIF 145

Query: 128 IG---QNLSSVFQGHGLAL---SSYIFLIAAVEVVLSWIGSLAALAPFSMFADICNAIAM 187
           +     NL S    H L L   S Y++     ++ L+ I SL  LAP S+FADI +  A 
Sbjct: 146 VATTMANLLSRGTEHILGLDAASIYLWGCFPFQLGLNSIPSLTHLAPLSIFADIVDVAAT 205

Query: 188 GIVVKEDIQKAIAGGISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAA 247
            +V+ +D+   +           ++    GL    G+AV+ FEG GM L LE   K K  
Sbjct: 206 LVVMVQDVFIFLKRRPPLRVFGGVSVFFYGL----GVAVYAFEGIGMVLPLELEAKYKDK 265

Query: 248 FPRVLAQAFVGITIVYVLFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLLFTF 307
           F R L  A   I+I+Y  FG  GYMAYG++T+DIIT NL     +  VQ+GL + L FTF
Sbjct: 266 FGRALGLAMGLISIMYGAFGLLGYMAYGEETKDIITTNLGTGVVSTLVQLGLAINLFFTF 325

Query: 308 PMMLHPINEIVEDKLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPG 367
           P+M+ P+ E+VE +L  S +                  + ++  AT+++ +  +A  VP 
Sbjct: 326 PLMMQPVYEVVERRLCSSRY------------------SVWVRWATVLV-VTLVALLVPN 385

Query: 368 FGVFASLVGSTVCALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTY 418
           F  F SLVGS+VC +L FVLP+ FHL    + LS  + V+   + + G++ A+ GT+
Sbjct: 386 FADFLSLVGSSVCVVLGFVLPSLFHLQAFKNELSITRIVVDVLVFLIGVMIAITGTW 416

BLAST of Lag0012217 vs. TAIR 10
Match: AT2G41190.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 121.7 bits (304), Expect = 1.4e-27
Identity = 102/406 (25.12%), Postives = 180/406 (44.33%), Query Frame = 0

Query: 23  STLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGVVLAGVATYYCMLLLVKCRGKLAS 82
           S +Q + N I  + G G+L  PY  + AGW +    ++ A +  Y   L+      K   
Sbjct: 148 SVIQTIFNAINVMAGVGLLSTPYTVKEAGWASMVILLLFAVICCYTATLMKDCFENKTG- 207

Query: 83  QGRSKESRTYGDLGYICMGNKGRYLTEFLIFISQCGGAVAYLVFIGQNLSSVFQGHGLAL 142
                   TY D+G    G  GR L   L++       V +++  G NL+ +F G  L L
Sbjct: 208 ------IITYPDIGEAAFGKYGRILICMLLYTELYSYCVEFIILEGDNLTGLFPGTSLDL 267

Query: 143 ------SSYIFLIAAVEVVLS--WIGSLAALAPFSMFADICNAIAMGIVVKEDIQKAIAG 202
                 S ++F I    +VL   W+  L  ++    +      IA  ++          G
Sbjct: 268 LGFRLDSKHLFGILTALIVLPTVWLKDLRIIS----YLSAGGVIATALIAVSVFFLGTTG 327

Query: 203 GISFEERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKAAFPRVLAQAFVGITI 262
           GI F   T       G+PFA G+  FC+ G  +   +  SM DK  F + +   F+   +
Sbjct: 328 GIGF-HHTGQAVKWNGIPFAIGIYGFCYSGHSVFPNIYQSMADKTKFNKAVITCFIICVL 387

Query: 263 VYVLFGFSGYMAYGDDTRDIITLNLP-NTWSTKAVQVGLCVGLLFTFPMMLHPINEIVED 322
           +Y      GY+ +G+ T   ITLN+P + + +K  Q    V     + ++++P+   +E+
Sbjct: 388 LYGGVAIMGYLMFGEATLSQITLNMPQDQFFSKVAQWTTVVSPFTKYALLMNPLARSIEE 447

Query: 323 KLNQSNWFGKIQDSDNVFSGKRIKVATYISRATIVIGLATLASFVPGFGVFASLVGSTVC 382
            L +       + S+N++          + R  +V      A  +P FG+  +L+GS + 
Sbjct: 448 LLPE-------RMSENIW-------CFLLLRTALVASSVCSAFLIPFFGLMMALIGSLLS 507

Query: 383 ALLSFVLPATFHLILMGSSLSFGQKVLSSFILICGLLFAVYGTYNT 420
            L++ ++PA   + +MG+  +  Q +LSS I+  G++    GTY++
Sbjct: 508 ILVAIIMPALCFIKIMGNKATRTQMILSSIIVAIGVVSGTLGTYSS 527