Lag0011952 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011952
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr1: 35370311 .. 35370637 (-)
RNA-Seq ExpressionLag0011952
SyntenyLag0011952
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAAGGTTAACGTAGATGCAGCTTGGACTTCTTCTCCTCCATCGACAGGGTTGAGGAAAATTATCAGAGATTCCATGGGCAACATAGTTGGAGCTTCAACATTTTCAGTTAAAATCGACTATAAAGCCCCCATAGCAGAAGTGATGGCAATTTTCAAAGGTGTATCGCTGGCTAGGTTGCTGGGATGTAAGGAAATTATCGTGGAATCTGATTCTTCTCAGGCGATTAATCTGGTGGCAAAGAAAACTGAAGTTTGGGGTTGGAGGCCATCTAGGATCAACTTGTTTTTTTTTCTCATATGTCAATTTTGTATACATTTCTAG

mRNA sequence

ATGTGGAAGGTTAACGTAGATGCAGCTTGGACTTCTTCTCCTCCATCGACAGGGTTGAGGAAAATTATCAGAGATTCCATGGGCAACATAGTTGGAGCTTCAACATTTTCAGTTAAAATCGACTATAAAGCCCCCATAGCAGAAGTGATGGCAATTTTCAAAGGTGTATCGCTGGCTAGGTTGCTGGGATGTAAGGAAATTATCGTGGAATCTGATTCTTCTCAGGCGATTAATCTGGTGGCAAAGAAAACTGAAGTTTGGGGTTGGAGGCCATCTAGGATCAACTTGTTTTTTTTTCTCATATGTCAATTTTGTATACATTTCTAG

Coding sequence (CDS)

ATGTGGAAGGTTAACGTAGATGCAGCTTGGACTTCTTCTCCTCCATCGACAGGGTTGAGGAAAATTATCAGAGATTCCATGGGCAACATAGTTGGAGCTTCAACATTTTCAGTTAAAATCGACTATAAAGCCCCCATAGCAGAAGTGATGGCAATTTTCAAAGGTGTATCGCTGGCTAGGTTGCTGGGATGTAAGGAAATTATCGTGGAATCTGATTCTTCTCAGGCGATTAATCTGGTGGCAAAGAAAACTGAAGTTTGGGGTTGGAGGCCATCTAGGATCAACTTGTTTTTTTTTCTCATATGTCAATTTTGTATACATTTCTAG

Protein sequence

MWKVNVDAAWTSSPPSTGLRKIIRDSMGNIVGASTFSVKIDYKAPIAEVMAIFKGVSLARLLGCKEIIVESDSSQAINLVAKKTEVWGWRPSRINLFFFLICQFCIHF
Homology
BLAST of Lag0011952 vs. NCBI nr
Match: XP_038902513.1 (uncharacterized protein LOC120089172 [Benincasa hispida])

HSP 1 Score: 66.2 bits (160), Expect = 1.9e-07
Identity = 39/105 (37.14%), Postives = 56/105 (53.33%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLRKIIRDSMGNIVGASTFSVKIDYKAPIAEVMAIFKGVSLARLL 62
           K+NVDAAW  SP S+G   IIRD+ G++      S+   Y  P+AE   + +G+ L   +
Sbjct: 65  KLNVDAAWKFSPYSSGFSAIIRDNQGSLKVVQISSIDAVYPPPLAEAFVVLQGLRLTSKM 124

Query: 63  GCKEIIVESDSSQAINLVAK------KTEVWGWRPSRINLFFFLI 102
             K+IIV+SD S AI+L  K         VW      I++ F+ I
Sbjct: 125 NFKKIIVKSDCSGAIDLFLKILISDSSVRVWLEEIWEISIVFYPI 169

BLAST of Lag0011952 vs. NCBI nr
Match: PNT66003.1 (hypothetical protein BRADI_3g05652v3 [Brachypodium distachyon])

HSP 1 Score: 59.3 bits (142), Expect = 2.4e-05
Identity = 28/83 (33.73%), Postives = 46/83 (55.42%), Query Frame = 0

Query: 1   MWKVNVDAAWTSSPPSTGLRKIIRDSMGNIVGASTFSVKIDYKAPIAEVMAIFKGVSLAR 60
           M K+NVD ++T          +IRD+ G  VGAS   + I Y A  AE +A++ G+ LA+
Sbjct: 127 MQKLNVDTSFTEDMDEGATGAVIRDASGMFVGASNSFIPIVYDAATAEALALWHGIQLAK 186

Query: 61  LLGCKEIIVESDSSQAINLVAKK 84
             GC  +++ SD  + ++ +  K
Sbjct: 187 NFGCSNLLINSDIIEVVHEMTSK 209

BLAST of Lag0011952 vs. NCBI nr
Match: PON66239.1 (Ribonuclease H-like domain containing protein [Parasponia andersonii])

HSP 1 Score: 59.3 bits (142), Expect = 2.4e-05
Identity = 28/83 (33.73%), Postives = 50/83 (60.24%), Query Frame = 0

Query: 2   WKVNVDAAWTSSPPSTGLRKIIRDSMGNIVGASTFSVKIDYKAPIAEVMAIFKGVSLARL 61
           +K+N DA+        GL  ++RD  G ++ A ++S  ++Y A + EVM + KG+ +AR 
Sbjct: 89  FKLNTDASLMVIGNKMGLGGVVRDYRGKVMVAFSYSHHLNYSAEVTEVMVVHKGIQIARD 148

Query: 62  LGCKEIIVESDSSQAINLVAKKT 85
            G   +IVESD+S  ++L+  ++
Sbjct: 149 NGLHPLIVESDASNVVSLIDNRS 171

BLAST of Lag0011952 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 59.3 bits (142), Expect = 2.4e-05
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 2   WKVNVDAAWTSSPPSTGLRKIIRDSMGNIVGASTFSVKIDYKAPIAEVMAIFKGVSLARL 61
           WK+N +AAW +   + G+  I+RD  G ++ AS   ++ +      EVMAI +G+   R 
Sbjct: 87  WKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQ 146

Query: 62  LGCKEIIVESDSSQAINLVAKKTE 86
             C+ I +ESDS +AI+L+ ++ +
Sbjct: 147 EHCRPIHLESDSLEAIHLLHRQCQ 170

BLAST of Lag0011952 vs. NCBI nr
Match: XP_030923260.1 (uncharacterized protein LOC115950147 [Quercus lobata])

HSP 1 Score: 59.3 bits (142), Expect = 2.4e-05
Identity = 32/81 (39.51%), Postives = 49/81 (60.49%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLRKIIRDSMGNIVGASTFSVKIDYKAPIAEVMAIFKGVSLARLL 62
           K+NVD A +    S+ +  +IRDS G +V A +  +++ + A ++E+MA+  GV LA+ L
Sbjct: 36  KINVDGASSELENSSSISVVIRDSFGKVVVALSKPLQVCFTAELSEIMALEHGVLLAQEL 95

Query: 63  GCKEIIVESDSSQAINLVAKK 84
               II+ESDS  AI  V  K
Sbjct: 96  QLSRIIIESDSQNAIQAVQDK 116

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902513.11.9e-0737.14uncharacterized protein LOC120089172 [Benincasa hispida][more]
PNT66003.12.4e-0533.73hypothetical protein BRADI_3g05652v3 [Brachypodium distachyon][more]
PON66239.12.4e-0533.73Ribonuclease H-like domain containing protein [Parasponia andersonii][more]
XP_022143535.12.4e-0534.52uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_030923260.12.4e-0539.51uncharacterized protein LOC115950147 [Quercus lobata][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..97
e-value: 5.9E-8
score: 34.8
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 5..85
e-value: 2.8E-13
score: 49.8
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 3..99
NoneNo IPR availablePANTHERPTHR47074:SF17PUTATIVE-RELATEDcoord: 3..99
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 4..108
e-value: 1.49296E-13
score: 60.0204
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 1..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011952.1Lag0011952.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity