Lag0011811 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011811
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionshort integuments 2, mitochondrial
Locationchr1: 33246290 .. 33249713 (-)
RNA-Seq ExpressionLag0011811
SyntenyLag0011811
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAAATGGCCACCTTAAACCCTAAACCTCCGTCGTCGTCATCGTCGGAGAGGGAGAAGATGAGGGGGATCAATGGAGTGGTGAAGAAGGCTTTAGGGGAGATGGAATTCACTGCCGGCGGTGGAGCCATCAACTGGTTCCCCGGCCACATGGCCGCAGCCACGCGAGCCATTCGCGACAGACTCAAGCTCGCGGATATGGTGATCGAGGTCAGAGACTCCCGAATTCCCTTGTCCTCGGCCAACCAAGACCTCCAGCCCCATCTCTCTTCTAAACGACGCATCATCGCCCTCAACAAGAAAGATTTGGCTAACCCTAATATCATGCACGTAAGACACCACTTGATGATTCTGCTTCATTTGATTAACTTTCTATCCGTTGGATTTGGAAGTATTAACTCTGAGTTCGGAAACAGCTAGAAAATGAGCAATGATTGGCTTCAAGTAGTCCTCCTGTGTGTATGTGTGTGCGTCTTTAATTATCCTTTATCTCCTTGTAGCTCTACAATTGAACTGTTTTGTTCACTACTAAGATTGGTTTCCAATGCTGAATGTTCTGTAAAGTGAGTTCACTCTGTATCTGTCTGTTGATCCTTTCTGAGAATTTCCATTTCAAATGTTTGTAAAGTGATATTTGTATGTGTACGAATGTGCTTATTTATATATGGGTGTATGTTGAAAGATAGATATTCAAATTACAAAAAGTTGGTTATTTGTCGTGTTCTTTTAAGTAGTTAATCGATCATTTTTCCTTCATAGTTCGCATTGTCATTACGAATTTAATTGTGCAGAAATGGATGCACTTTTTTGACTCGTGCAATCAAGATTGCATTCCAATTAACGCTCACAGCAAGAGTTCTGTTCGGAAGGTATTGTTGTTTTCTACAGTACTGCGGTGATGTTAATTTACAGCTTCCAATTAATCTGAGGTTCTGGCCTTTAATATACAGCTTGATATTAATTTATTAGTTATTATTTTTTTATGGATGAAAAAGCTTCTTGAGCTCGTGGAATTTAAACTAAAGGAAGCAATTTCAAGGGAGCCTACTCTTCTTGTTATGGTGGTTGGTGTTCCGAATGTTGGGAAGTCGGCTTTAATTAATTCAATCCATCAAATTGCTTCTGCACGCTTTCCAGGTAAGGCTGTTTCTTGGAACATTATGTCAATTCCTCTTTCATATTGAATTGTACAAGATTACAAGTATCCGGAGGCCTTGTTTGTTGGACTTTTGTGGTTTCAATAATTTTTCCATAGTGCAGGAGAAAATGAAGCGAGCTCATGTTGGTCCCTTGCCTGGGGTTACTCAAGATATTGCTGGCTACAAGGTAGTTTTGATTAACCGCCTATCTTCATTTTGATTAATCAATTTCTACTTTTGCCTCTCCTTTCCTTTCTTCTTTCTGATATGAGTTCTTATCATCTTCAATTATTAACCATTTGCAATTCTTTGATATGCAGATTGCTCATCAACCTAGCATATATGTTCTAGATACTCCCGGTGTATTAGTTCCAAGTATTCAAGATATCGAGACAGGGTTAAAACTGGCACTTGCAGGTTGAATTCTAGTTTATAAGAATTATTTTTTATTTTTCAGAAACGTTTCATTAAGCTATAGACTATAGCTCTATGTGCATTGGGATATGCTATACAAGAATGATGCAATTAGGTGAAATACTGATTGTGCCGTTTATGCCACTAAATAACTAGTGCTGCTGAAGTTAAAAACATGGACTACGTAATGAATAATTACTAATGAAATCTGACCACAATAACCTAAATCTTAAGGACAGAAACTTCTTAGAGTATTGTAATTTTTAGTCTAGACAATTTGATGGAACATGAAATTCGCATCCCCTGCCAACTCCCATCTTGCAAGCTTTTAGCAGGATCGTGATTAGATGGATGCTGAAACAGTAATCTTGTGTTGTGATAATGCTTGTGTTTGGAGTTATGATTTTATCTCCTCGTGGCACCTCTTTGTGGGGACGGAAGATTCTTTCTTCATGTGATTGCTGGATTCCAATGTCCACAACCCCACAGTAGCCTTTTTGTTTGAATGGGATCTTTCTGTTGCCTATTTCCTCAATCATACAATTGAGATTAGTTCATTAAACTTACGGTTTAGTTTCCTCTTCATTCTAATATTGCACCATCACGTTGATATTGGCCATATTAGTGCATCTCCTTGTAAAACTGACGCTATATATATATTATTTTACATGGAAGCAGGATCTGTGAAGGATGCCGTGGTGGGTGAGGAGCGTATTGCTCAATATCTGCTGGCCGTTCTGAATACTCGGGGGACTCCCTTCCATTGGAAGCAGTTGAATAACAGAAGAATGGAGGGTATTAAATATGAACCAGAGGAAGGGCACAGATTTAATCTGAATGATCTCCGGCCAAGAAGGAGGGCTCCTCCTCCAAATAAGTCTGATGTGGTATACATTGAGGTAAGGAAAGGATTTCTTATTCAGACTCAGTGTCTGTGCCTTTCTATGTTCCATGGGACTTTAATGAATTGTAGTTTACAATTAGAGGGAATTCCAGATTTAAATTGTTCACCAGAAACAGGGAAAGACGTTAATTGTTGCTCTCTCGATAATATTGTGACAGTGCTTCCTCTTAGAGCTTGATGCTTTTATGGCATAGGATTAGCAAGTTAATTGGGCTTCAGTCTACTGGTTGGGATTCAATTTTAGTCGTTGAACTTTAAAAAACACATGGAAAGCATGAACATGAGCTGAGTTCAAAATCTTTACTTTCTCTAATGGGTTGTAGGTTCGAATCCTCACCCCGTGATGATATAATTTCTTGAAAAAAAGAACAAAATAAAACAAACTGAAAGCTACTTGCCCTGTTTTCTTAGCTTTATTTTCTGTTTGGGAGGATCAGGGAATGGCAATGACTCTATTGTTTTTTTTTTTTTTTATTTCCTAGCATCTTTAATATTTACTTGCAAAAAATTATCCTCTCTCTTCTTTCATCTACTACTCTGGAATCCGGGGTCCTGGAGGTGGTCAAGTTGCAGACTCTTTTATCCATCATTGGGTGGTTCTTGTATAACCTCACTTTTTCTCTTACTTTCATTCCTCTTCACAAAAGCGGTCTATAAACCGAAAAGGAAAACTGTATTCTTATTATCTGATTCATTTGTTCAGGATCTTGTTACAGAAGTTCAACGTGCACTGTACGCAACTCTGTCGAAATTTGATGGTAATGTAGAAGATGAGAATGATTTAGAAAGTCTTATCGAACTGCAGTTTGAAGCATTGCAGAAAGCAATGAAGATACCTCACAAGGCAGCAGAAGCTCGGCTGAAAGTATCAAAGAAACTACTTACTCTATTCAGAGCCGGTAAGCTTGGTCCATTCATCCTTGATGATGTCCCTAACGTAAATTATTCACCATTAAATCCTTAG

mRNA sequence

ATGGCAAAAATGGCCACCTTAAACCCTAAACCTCCGTCGTCGTCATCGTCGGAGAGGGAGAAGATGAGGGGGATCAATGGAGTGGTGAAGAAGGCTTTAGGGGAGATGGAATTCACTGCCGGCGGTGGAGCCATCAACTGGTTCCCCGGCCACATGGCCGCAGCCACGCGAGCCATTCGCGACAGACTCAAGCTCGCGGATATGGTGATCGAGGTCAGAGACTCCCGAATTCCCTTGTCCTCGGCCAACCAAGACCTCCAGCCCCATCTCTCTTCTAAACGACGCATCATCGCCCTCAACAAGAAAGATTTGGCTAACCCTAATATCATGCACAAATGGATGCACTTTTTTGACTCGTGCAATCAAGATTGCATTCCAATTAACGCTCACAGCAAGAGTTCTGTTCGGAAGCTTCTTGAGCTCGTGGAATTTAAACTAAAGGAAGCAATTTCAAGGGAGCCTACTCTTCTTGTTATGGTGGTTGGTGTTCCGAATGTTGGGAAGTCGGCTTTAATTAATTCAATCCATCAAATTGCTTCTGCACGCTTTCCAGTGCAGGAGAAAATGAAGCGAGCTCATGTTGGTCCCTTGCCTGGGGTTACTCAAGATATTGCTGGCTACAAGATTGCTCATCAACCTAGCATATATGTTCTAGATACTCCCGGTGTATTAGTTCCAAGTATTCAAGATATCGAGACAGGGTTAAAACTGGCACTTGCAGGATCTGTGAAGGATGCCGTGGTGGGTGAGGAGCGTATTGCTCAATATCTGCTGGCCGTTCTGAATACTCGGGGGACTCCCTTCCATTGGAAGCAGTTGAATAACAGAAGAATGGAGGGTATTAAATATGAACCAGAGGAAGGGCACAGATTTAATCTGAATGATCTCCGGCCAAGAAGGAGGGCTCCTCCTCCAAATAAGTCTGATGTGGTATACATTGAGGATCTTGTTACAGAAGTTCAACGTGCACTGTACGCAACTCTGTCGAAATTTGATGGTAATGTAGAAGATGAGAATGATTTAGAAAGTCTTATCGAACTGCAGTTTGAAGCATTGCAGAAAGCAATGAAGATACCTCACAAGGCAGCAGAAGCTCGGCTGAAAGTATCAAAGAAACTACTTACTCTATTCAGAGCCGGTAAGCTTGGTCCATTCATCCTTGATGATGTCCCTAACGTAAATTATTCACCATTAAATCCTTAG

Coding sequence (CDS)

ATGGCAAAAATGGCCACCTTAAACCCTAAACCTCCGTCGTCGTCATCGTCGGAGAGGGAGAAGATGAGGGGGATCAATGGAGTGGTGAAGAAGGCTTTAGGGGAGATGGAATTCACTGCCGGCGGTGGAGCCATCAACTGGTTCCCCGGCCACATGGCCGCAGCCACGCGAGCCATTCGCGACAGACTCAAGCTCGCGGATATGGTGATCGAGGTCAGAGACTCCCGAATTCCCTTGTCCTCGGCCAACCAAGACCTCCAGCCCCATCTCTCTTCTAAACGACGCATCATCGCCCTCAACAAGAAAGATTTGGCTAACCCTAATATCATGCACAAATGGATGCACTTTTTTGACTCGTGCAATCAAGATTGCATTCCAATTAACGCTCACAGCAAGAGTTCTGTTCGGAAGCTTCTTGAGCTCGTGGAATTTAAACTAAAGGAAGCAATTTCAAGGGAGCCTACTCTTCTTGTTATGGTGGTTGGTGTTCCGAATGTTGGGAAGTCGGCTTTAATTAATTCAATCCATCAAATTGCTTCTGCACGCTTTCCAGTGCAGGAGAAAATGAAGCGAGCTCATGTTGGTCCCTTGCCTGGGGTTACTCAAGATATTGCTGGCTACAAGATTGCTCATCAACCTAGCATATATGTTCTAGATACTCCCGGTGTATTAGTTCCAAGTATTCAAGATATCGAGACAGGGTTAAAACTGGCACTTGCAGGATCTGTGAAGGATGCCGTGGTGGGTGAGGAGCGTATTGCTCAATATCTGCTGGCCGTTCTGAATACTCGGGGGACTCCCTTCCATTGGAAGCAGTTGAATAACAGAAGAATGGAGGGTATTAAATATGAACCAGAGGAAGGGCACAGATTTAATCTGAATGATCTCCGGCCAAGAAGGAGGGCTCCTCCTCCAAATAAGTCTGATGTGGTATACATTGAGGATCTTGTTACAGAAGTTCAACGTGCACTGTACGCAACTCTGTCGAAATTTGATGGTAATGTAGAAGATGAGAATGATTTAGAAAGTCTTATCGAACTGCAGTTTGAAGCATTGCAGAAAGCAATGAAGATACCTCACAAGGCAGCAGAAGCTCGGCTGAAAGTATCAAAGAAACTACTTACTCTATTCAGAGCCGGTAAGCTTGGTCCATTCATCCTTGATGATGTCCCTAACGTAAATTATTCACCATTAAATCCTTAG

Protein sequence

MAKMATLNPKPPSSSSSEREKMRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVLNTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGKLGPFILDDVPNVNYSPLNP
Homology
BLAST of Lag0011811 vs. NCBI nr
Match: XP_022966362.1 (short integuments 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 699.5 bits (1804), Expect = 1.7e-197
Identity = 357/388 (92.01%), Postives = 373/388 (96.13%), Query Frame = 0

Query: 4   MATLNPKPPSSSSSEREKMRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRL 63
           MATLNPKPP SS  ERE+M+GI GVVKKALGEMEFTA GGAINWFPGHMAAATRAIRDR+
Sbjct: 1   MATLNPKPP-SSLLERERMKGITGVVKKALGEMEFTAAGGAINWFPGHMAAATRAIRDRV 60

Query: 64  KLADMVIEVRDSRIPLSSANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQD 123
           KLADMVIEVRDSRIPLSSANQDLQ HLSSKRRIIALNKKDLANPNIMHKW HFF+SCNQD
Sbjct: 61  KLADMVIEVRDSRIPLSSANQDLQSHLSSKRRIIALNKKDLANPNIMHKWTHFFESCNQD 120

Query: 124 CIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARF 183
           CIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMV+GVPNVGKSALINSIHQIASARF
Sbjct: 121 CIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMVIGVPNVGKSALINSIHQIASARF 180

Query: 184 PVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSV 243
           PVQEKMKRA+VGPLPGVTQDIAG+KIAHQPSIYVLDTPGVLVPSI+D+ETGLKL+LAGSV
Sbjct: 181 PVQEKMKRANVGPLPGVTQDIAGFKIAHQPSIYVLDTPGVLVPSIKDVETGLKLSLAGSV 240

Query: 244 KDAVVGEERIAQYLLAVLNTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAP 303
           KD+VVGEERIAQYLLAVLNTRGTPFHWKQ NNRR EGI+YE EE HRF+LNDLRP+RR P
Sbjct: 241 KDSVVGEERIAQYLLAVLNTRGTPFHWKQSNNRRTEGIQYEAEEMHRFSLNDLRPKRRPP 300

Query: 304 PPNKSDVVYIEDLVTEVQRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAA 363
            PNKSDVVY+EDLVT+VQRALYA LS FDGNVEDENDLESLIELQFEALQKAMKIPHKAA
Sbjct: 301 LPNKSDVVYVEDLVTQVQRALYAMLSGFDGNVEDENDLESLIELQFEALQKAMKIPHKAA 360

Query: 364 EARLKVSKKLLTLFRAGKLGPFILDDVP 392
           EARLKVSKKLLTLFRAGKLGPFILDDVP
Sbjct: 361 EARLKVSKKLLTLFRAGKLGPFILDDVP 387

BLAST of Lag0011811 vs. NCBI nr
Match: XP_038883550.1 (short integuments 2, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 691.8 bits (1784), Expect = 3.5e-195
Identity = 350/370 (94.59%), Postives = 363/370 (98.11%), Query Frame = 0

Query: 22  MRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSS 81
           MRGINGVVKKALG+MEFTAGGGA+NWFPGHMAAATRAIR RLKLADMVIEVRDSRIPLSS
Sbjct: 1   MRGINGVVKKALGDMEFTAGGGAVNWFPGHMAAATRAIRHRLKLADMVIEVRDSRIPLSS 60

Query: 82  ANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL 141
           ANQDLQPHLSSKRR+IALNKKDLANPNIMHKW+HFFDSCNQDCIPINAHSKSSVRKLLEL
Sbjct: 61  ANQDLQPHLSSKRRVIALNKKDLANPNIMHKWVHFFDSCNQDCIPINAHSKSSVRKLLEL 120

Query: 142 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVT 201
           VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIAS RFPVQEKMKRA VGPLPGVT
Sbjct: 121 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASERFPVQEKMKRATVGPLPGVT 180

Query: 202 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 261
           QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL
Sbjct: 181 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 240

Query: 262 NTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQ 321
           N+RGTPFHWK LNNRRMEGI+YEPEE HRFNLNDL+P+RRA PPNKSDV+YIEDLVTEVQ
Sbjct: 241 NSRGTPFHWKHLNNRRMEGIQYEPEERHRFNLNDLQPKRRA-PPNKSDVLYIEDLVTEVQ 300

Query: 322 RALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 381
           RALY T+S+FDGNVEDENDLESLIE+QFEALQKAMKIPHKAAEARL+VSKKLLTLFRAGK
Sbjct: 301 RALYTTVSEFDGNVEDENDLESLIEVQFEALQKAMKIPHKAAEARLRVSKKLLTLFRAGK 360

Query: 382 LGPFILDDVP 392
           LGPFILDDVP
Sbjct: 361 LGPFILDDVP 369

BLAST of Lag0011811 vs. NCBI nr
Match: XP_022156767.1 (short integuments 2, mitochondrial isoform X1 [Momordica charantia] >XP_022156768.1 short integuments 2, mitochondrial isoform X1 [Momordica charantia] >XP_022156769.1 short integuments 2, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 690.6 bits (1781), Expect = 7.7e-195
Identity = 351/385 (91.17%), Postives = 365/385 (94.81%), Query Frame = 0

Query: 15  SSSEREKMRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRD 74
           S     +M GI G+VKKALGEMEFTAGGGAINWFPGHMAAATRAIR+RLKLADMVIEVRD
Sbjct: 3   SIKNMHRMGGIKGLVKKALGEMEFTAGGGAINWFPGHMAAATRAIRERLKLADMVIEVRD 62

Query: 75  SRIPLSSANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSS 134
           +RIPLSSANQDLQP LSSKRRIIALNKKDLANPNIMHKWM+FFDSCNQDCI INAHSKSS
Sbjct: 63  ARIPLSSANQDLQPQLSSKRRIIALNKKDLANPNIMHKWMYFFDSCNQDCISINAHSKSS 122

Query: 135 VRKLLELVEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHV 194
           V+KLLEL EFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRA+V
Sbjct: 123 VQKLLELAEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRANV 182

Query: 195 GPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIA 254
           GPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIA
Sbjct: 183 GPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIA 242

Query: 255 QYLLAVLNTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIE 314
           QYLLAVLN RGTPFHWKQLNNRRMEGI+YE EE H+FNLNDLRP+R APP NKSDVVY+E
Sbjct: 243 QYLLAVLNIRGTPFHWKQLNNRRMEGIQYESEERHKFNLNDLRPKRGAPPTNKSDVVYVE 302

Query: 315 DLVTEVQRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLL 374
           DLVTEVQRALY +LS+FDGNVEDENDLESLIELQFEALQKAMKIPHKAAE RLKVSKKLL
Sbjct: 303 DLVTEVQRALYTSLSEFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEGRLKVSKKLL 362

Query: 375 TLFRAGKLGPFILDDVPNVNYSPLN 400
           TLFRAGKLGPFILDDVP +  SPLN
Sbjct: 363 TLFRAGKLGPFILDDVPTIKSSPLN 387

BLAST of Lag0011811 vs. NCBI nr
Match: KAG6595673.1 (Short integuments 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 682.6 bits (1760), Expect = 2.1e-192
Identity = 345/373 (92.49%), Postives = 361/373 (96.78%), Query Frame = 0

Query: 22  MRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSS 81
           M+GI GVVKKALGEMEFTA GGAINWFPGHMAAATRAIRDR+KLADMVIEVRDSRIPLSS
Sbjct: 1   MKGITGVVKKALGEMEFTAAGGAINWFPGHMAAATRAIRDRVKLADMVIEVRDSRIPLSS 60

Query: 82  ANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL 141
           ANQDLQ HLSSKRRIIALNKKDLANPNIMHKW HFF+SCNQDCIPINAHS SSVRKLLEL
Sbjct: 61  ANQDLQSHLSSKRRIIALNKKDLANPNIMHKWTHFFESCNQDCIPINAHSMSSVRKLLEL 120

Query: 142 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVT 201
           VEFKLKEAISREPTLLVMV+GVPNVGKSALINSIHQIASARFPVQEKMKRA+VGPLPGVT
Sbjct: 121 VEFKLKEAISREPTLLVMVIGVPNVGKSALINSIHQIASARFPVQEKMKRANVGPLPGVT 180

Query: 202 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 261
           QDIAG+KIAHQPSIYVLDTPGVLVPSI+D+ETGLKLALAGSVKD+VVGEERIAQYLLAVL
Sbjct: 181 QDIAGFKIAHQPSIYVLDTPGVLVPSIKDVETGLKLALAGSVKDSVVGEERIAQYLLAVL 240

Query: 262 NTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQ 321
           NTRGTPFHWKQ NNRRMEGI+YE EE HRF+LNDLRP+RR P PNKSDVVY+EDLVT+VQ
Sbjct: 241 NTRGTPFHWKQSNNRRMEGIQYESEEMHRFSLNDLRPKRRPPLPNKSDVVYVEDLVTQVQ 300

Query: 322 RALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 381
           RALYATLS FDGNVEDENDLESLIE+QFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK
Sbjct: 301 RALYATLSGFDGNVEDENDLESLIEVQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 360

Query: 382 LGPFILDDVPNVN 395
           LGPFILDDVP+ N
Sbjct: 361 LGPFILDDVPSHN 373

BLAST of Lag0011811 vs. NCBI nr
Match: XP_022924906.1 (short integuments 2, mitochondrial [Cucurbita moschata] >XP_022924907.1 short integuments 2, mitochondrial [Cucurbita moschata] >XP_022924908.1 short integuments 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 681.0 bits (1756), Expect = 6.1e-192
Identity = 344/373 (92.23%), Postives = 360/373 (96.51%), Query Frame = 0

Query: 22  MRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSS 81
           M+GI GVVKKALGEMEFTA GGAINWFPGHMAAATRAIRDR+KLADMVIEVRDSRIPLSS
Sbjct: 1   MKGITGVVKKALGEMEFTAAGGAINWFPGHMAAATRAIRDRVKLADMVIEVRDSRIPLSS 60

Query: 82  ANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL 141
           ANQDLQ HLSSKRRIIALNKKDLANPNIMHKW HFF+SCNQDCIPINAHS SSVRKLLEL
Sbjct: 61  ANQDLQSHLSSKRRIIALNKKDLANPNIMHKWTHFFESCNQDCIPINAHSMSSVRKLLEL 120

Query: 142 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVT 201
           VEFKLKEAISREPTLLVMV+GVPNVGKSALINSIHQIASARFPVQEKMKRA+VGPLPGVT
Sbjct: 121 VEFKLKEAISREPTLLVMVIGVPNVGKSALINSIHQIASARFPVQEKMKRANVGPLPGVT 180

Query: 202 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 261
           QDIAG+KIAHQPSIYVLDTPGVLVPSI+D+ETGLKLALAGSVKD+VVGEERIAQYLLAVL
Sbjct: 181 QDIAGFKIAHQPSIYVLDTPGVLVPSIKDVETGLKLALAGSVKDSVVGEERIAQYLLAVL 240

Query: 262 NTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQ 321
           NTRGTPFHWKQ NNRRMEGI+YE EE HRF+LNDLRP+RR P PNKSDVVY+EDLVT+VQ
Sbjct: 241 NTRGTPFHWKQSNNRRMEGIQYESEEMHRFSLNDLRPKRRPPLPNKSDVVYVEDLVTQVQ 300

Query: 322 RALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 381
           RALYAT S FDGNVEDENDLESLIE+QFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK
Sbjct: 301 RALYATFSGFDGNVEDENDLESLIEVQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 360

Query: 382 LGPFILDDVPNVN 395
           LGPFILDDVP+ N
Sbjct: 361 LGPFILDDVPSHN 373

BLAST of Lag0011811 vs. ExPASy Swiss-Prot
Match: Q8L607 (Short integuments 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SIN2 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 4.0e-146
Identity = 266/364 (73.08%), Postives = 306/364 (84.07%), Query Frame = 0

Query: 28  VVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQ 87
           V K  +G M F    G INWFPGHMAAATRAIR+RLKL+D+VIEVRD+RIPLSSAN+DLQ
Sbjct: 9   VKKGLIGGMSFAKDAGKINWFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQ 68

Query: 88  PHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLELVEFKLK 147
             +S+KRRIIALNKKDLANPN+++KW   F+S  QDCI INAHS+SSV KLL+LVE KLK
Sbjct: 69  SQMSAKRRIIALNKKDLANPNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLK 128

Query: 148 EAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGY 207
           E I+REPTLLVMVVGVPNVGKSALINSIHQIA+ARFPVQE++KRA VGPLPGVTQDIAG+
Sbjct: 129 EVIAREPTLLVMVVGVPNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGF 188

Query: 208 KIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVLNTRGTP 267
           KIAH+PSIYVLD+PGVLVPSI DIETGLKLAL+GSVKD+VVGEERIAQY LA+LN RGTP
Sbjct: 189 KIAHRPSIYVLDSPGVLVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTP 248

Query: 268 FHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQRALYAT 327
            HWK L     EG   +  +   +NL DLR  +R   P+ S + Y+ D+++EVQR+LY T
Sbjct: 249 LHWKYLVEGINEGPHADCIDKPSYNLKDLR-HQRTKQPDSSALHYVGDMISEVQRSLYIT 308

Query: 328 LSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGKLGPFIL 387
           LS+FDG+ EDENDLE LIE QFE LQKA+KIPHKA+EARL VSKK LTLFR G+LGPFIL
Sbjct: 309 LSEFDGDTEDENDLECLIEQQFEVLQKALKIPHKASEARLMVSKKFLTLFRTGRLGPFIL 368

Query: 388 DDVP 392
           DDVP
Sbjct: 369 DDVP 371

BLAST of Lag0011811 vs. ExPASy Swiss-Prot
Match: O82497 (DAR GTPase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DGP2 PE=3 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.7e-59
Identity = 138/352 (39.20%), Postives = 212/352 (60.23%), Query Frame = 0

Query: 47  WFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQPH--LSSKRRIIALNKKDL 106
           W+  HMAAA RAI +R+ L D V+E+RD+RIPLSS  + L+    L SK RII LNK +L
Sbjct: 28  WYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYELLRKFSPLPSK-RIIVLNKMEL 87

Query: 107 ANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLELVEFKLKE---AISREPTLLVMVV 166
           A+P  + K + +F+  N     +N+H+K  V++LL  ++ +++E   A     T  +M++
Sbjct: 88  ADPLELKKCIDYFEERNYLSYAVNSHNKDCVKQLLNFLQSQVRELHKAGHSGHTTTMMLL 147

Query: 167 GVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIYVLDTP 226
           G+PNVGKSAL NS+H I       + K+K   V   PG T+DI   KI   P++YVLDTP
Sbjct: 148 GIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGDTKDIMSLKIGSHPNVYVLDTP 207

Query: 227 GVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVLNTRGTPFHWKQL--NNRRME 286
           G+  P++ D E   KLAL G++ D +VGE ++A+  L +LN+      W +L  +    E
Sbjct: 208 GIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTILNSSHEYKKWAKLCKSQDLTE 267

Query: 287 GIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQRALYATLSKFDGNVEDEN 346
            +  E  +      +D + +R+    +  D +     V +V+R LY T+S FDGN+EDE 
Sbjct: 268 SLSDESSK------SDAKHKRQYATDHTQDFI-----VYDVRRVLYETISAFDGNLEDEI 327

Query: 347 DLESLIELQFEALQKAMKIPHKAAE-ARLKVSKKLLTLFRAGKLGPFILDDV 391
            + +LIE QF AL+  +++P +A+E A L+V+ K+L L+R G+LG + L+ V
Sbjct: 328 SMGNLIETQFAALRSVLRVPEEASEFADLRVASKILNLYRTGRLGHYTLEHV 367

BLAST of Lag0011811 vs. ExPASy Swiss-Prot
Match: Q8R2R6 (Mitochondrial ribosome-associated GTPase 1 OS=Mus musculus OX=10090 GN=Mtg1 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.5e-36
Identity = 99/250 (39.60%), Postives = 133/250 (53.20%), Query Frame = 0

Query: 27  GVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDL 86
           G V+ A  E     G     WFPGHMA   + ++  LK  D VIEV D+RIP S  N   
Sbjct: 9   GAVRGAWRECFPLQGHDVARWFPGHMAKGLKKMQSSLKSVDCVIEVHDARIPFSGRNPLF 68

Query: 87  QPHLSSKRRIIALNKKDLANPNIMHKWMH----------FFDSCNQDCIPINAHSKSSVR 146
           Q  L  K  ++ LNK DLA+     K +            F +C +D      + K  V 
Sbjct: 69  QELLGLKPHLLVLNKMDLADLTEQQKIVQRLEEKGLSNVLFTNCVKD-----ENIKQIVP 128

Query: 147 KLLELVEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGP 206
           K++EL+    +   +  P   +MVVGVPNVGKS+LINS+      R       K A VG 
Sbjct: 129 KVMELIRCSYRYHRAETPEYCIMVVGVPNVGKSSLINSL------RRQHLRTGKAARVGG 188

Query: 207 LPGVTQDIAG-YKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQ 266
            PG+T+ +    ++  +P +++LDTPGVL P I+ +ETGLKLAL G+V D +VGEE +A 
Sbjct: 189 EPGITRAVTSRIQVCERPLVFLLDTPGVLAPRIESVETGLKLALCGTVLDHLVGEETMAD 247

BLAST of Lag0011811 vs. ExPASy Swiss-Prot
Match: Q9BT17 (Mitochondrial ribosome-associated GTPase 1 OS=Homo sapiens OX=9606 GN=MTG1 PE=1 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 2.4e-34
Identity = 93/228 (40.79%), Postives = 131/228 (57.46%), Query Frame = 0

Query: 41  GGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQPHLSSKRRIIALN 100
           G     WFPGHMA   + ++  LKL D +IEV D+RIPLS  N   Q  L  K  ++ LN
Sbjct: 24  GRDVARWFPGHMAKGLKKMQSSLKLVDCIIEVHDARIPLSGRNPLFQETLGLKPHLLVLN 83

Query: 101 KKDLANPNIMHKWM-HFFDSCNQDCIPINAHSKSSVRKLLELVEFKL--KEAISREPTL- 160
           K DLA+     K M H      ++ I  N     +V++++ +V   +       R+  L 
Sbjct: 84  KMDLADLTEQQKIMQHLEGEGLKNVIFTNCVKDENVKQIIPMVTELIGRSHRYHRKENLE 143

Query: 161 -LVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDI-AGYKIAHQPS 220
             +MV+GVPNVGKS+LINS+      R     K K   VG  PG+T+ + +  +++ +P 
Sbjct: 144 YCIMVIGVPNVGKSSLINSL------RRQHLRKGKATRVGGEPGITRAVMSKIQVSERPL 203

Query: 221 IYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVLN 263
           +++LDTPGVL P I+ +ETGLKLAL G+V D +VGEE +A YLL  LN
Sbjct: 204 MFLLDTPGVLAPRIESVETGLKLALCGTVLDHLVGEETMADYLLYTLN 245

BLAST of Lag0011811 vs. ExPASy Swiss-Prot
Match: B7GGD6 (Ribosome biogenesis GTPase A OS=Anoxybacillus flavithermus (strain DSM 21510 / WK1) OX=491915 GN=rbgA PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.2e-34
Identity = 83/225 (36.89%), Postives = 132/225 (58.67%), Query Frame = 0

Query: 45  INWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQPHLSSKRRIIALNKKDL 104
           I WFPGHMA A R + ++LKL D+V E+ D+RIP+SS N  +   +++K RII LNK D+
Sbjct: 6   IQWFPGHMAKAKREVTEKLKLIDIVFELVDARIPMSSRNPLIDEIVANKPRIILLNKADM 65

Query: 105 ANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLELVEFKLK--------EAISREPTL 164
           A+P++  +W+ FF +   D I I++ S + V++++ + + KL+        + + R   +
Sbjct: 66  ADPDVTKQWVDFFAAQQIDAIAIDSQSGTGVKQMVAVAKEKLRSKFEKMMAKGMKRPRAM 125

Query: 165 LVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIY 224
             ++VG+PNVGKS LIN   ++A            A  G  PGVT+     K+  +  + 
Sbjct: 126 RALIVGIPNVGKSTLIN---RLAGKHI--------AKTGDTPGVTKAQQWIKVGKE--LE 185

Query: 225 VLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 262
           +LDTPG+L P  +D E G KLA  G++KD ++  + +A Y L  L
Sbjct: 186 LLDTPGILWPKFEDEEVGYKLATTGAIKDTILNLQDVAVYALRFL 217

BLAST of Lag0011811 vs. ExPASy TrEMBL
Match: A0A6J1HMR9 (short integuments 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111466034 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 8.0e-198
Identity = 357/388 (92.01%), Postives = 373/388 (96.13%), Query Frame = 0

Query: 4   MATLNPKPPSSSSSEREKMRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRL 63
           MATLNPKPP SS  ERE+M+GI GVVKKALGEMEFTA GGAINWFPGHMAAATRAIRDR+
Sbjct: 1   MATLNPKPP-SSLLERERMKGITGVVKKALGEMEFTAAGGAINWFPGHMAAATRAIRDRV 60

Query: 64  KLADMVIEVRDSRIPLSSANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQD 123
           KLADMVIEVRDSRIPLSSANQDLQ HLSSKRRIIALNKKDLANPNIMHKW HFF+SCNQD
Sbjct: 61  KLADMVIEVRDSRIPLSSANQDLQSHLSSKRRIIALNKKDLANPNIMHKWTHFFESCNQD 120

Query: 124 CIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARF 183
           CIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMV+GVPNVGKSALINSIHQIASARF
Sbjct: 121 CIPINAHSKSSVRKLLELVEFKLKEAISREPTLLVMVIGVPNVGKSALINSIHQIASARF 180

Query: 184 PVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSV 243
           PVQEKMKRA+VGPLPGVTQDIAG+KIAHQPSIYVLDTPGVLVPSI+D+ETGLKL+LAGSV
Sbjct: 181 PVQEKMKRANVGPLPGVTQDIAGFKIAHQPSIYVLDTPGVLVPSIKDVETGLKLSLAGSV 240

Query: 244 KDAVVGEERIAQYLLAVLNTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAP 303
           KD+VVGEERIAQYLLAVLNTRGTPFHWKQ NNRR EGI+YE EE HRF+LNDLRP+RR P
Sbjct: 241 KDSVVGEERIAQYLLAVLNTRGTPFHWKQSNNRRTEGIQYEAEEMHRFSLNDLRPKRRPP 300

Query: 304 PPNKSDVVYIEDLVTEVQRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAA 363
            PNKSDVVY+EDLVT+VQRALYA LS FDGNVEDENDLESLIELQFEALQKAMKIPHKAA
Sbjct: 301 LPNKSDVVYVEDLVTQVQRALYAMLSGFDGNVEDENDLESLIELQFEALQKAMKIPHKAA 360

Query: 364 EARLKVSKKLLTLFRAGKLGPFILDDVP 392
           EARLKVSKKLLTLFRAGKLGPFILDDVP
Sbjct: 361 EARLKVSKKLLTLFRAGKLGPFILDDVP 387

BLAST of Lag0011811 vs. ExPASy TrEMBL
Match: A0A6J1DRI9 (short integuments 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023602 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.7e-195
Identity = 351/385 (91.17%), Postives = 365/385 (94.81%), Query Frame = 0

Query: 15  SSSEREKMRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRD 74
           S     +M GI G+VKKALGEMEFTAGGGAINWFPGHMAAATRAIR+RLKLADMVIEVRD
Sbjct: 3   SIKNMHRMGGIKGLVKKALGEMEFTAGGGAINWFPGHMAAATRAIRERLKLADMVIEVRD 62

Query: 75  SRIPLSSANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSS 134
           +RIPLSSANQDLQP LSSKRRIIALNKKDLANPNIMHKWM+FFDSCNQDCI INAHSKSS
Sbjct: 63  ARIPLSSANQDLQPQLSSKRRIIALNKKDLANPNIMHKWMYFFDSCNQDCISINAHSKSS 122

Query: 135 VRKLLELVEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHV 194
           V+KLLEL EFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRA+V
Sbjct: 123 VQKLLELAEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRANV 182

Query: 195 GPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIA 254
           GPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIA
Sbjct: 183 GPLPGVTQDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIA 242

Query: 255 QYLLAVLNTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIE 314
           QYLLAVLN RGTPFHWKQLNNRRMEGI+YE EE H+FNLNDLRP+R APP NKSDVVY+E
Sbjct: 243 QYLLAVLNIRGTPFHWKQLNNRRMEGIQYESEERHKFNLNDLRPKRGAPPTNKSDVVYVE 302

Query: 315 DLVTEVQRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLL 374
           DLVTEVQRALY +LS+FDGNVEDENDLESLIELQFEALQKAMKIPHKAAE RLKVSKKLL
Sbjct: 303 DLVTEVQRALYTSLSEFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEGRLKVSKKLL 362

Query: 375 TLFRAGKLGPFILDDVPNVNYSPLN 400
           TLFRAGKLGPFILDDVP +  SPLN
Sbjct: 363 TLFRAGKLGPFILDDVPTIKSSPLN 387

BLAST of Lag0011811 vs. ExPASy TrEMBL
Match: A0A6J1EAB7 (short integuments 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111432314 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.0e-192
Identity = 344/373 (92.23%), Postives = 360/373 (96.51%), Query Frame = 0

Query: 22  MRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSS 81
           M+GI GVVKKALGEMEFTA GGAINWFPGHMAAATRAIRDR+KLADMVIEVRDSRIPLSS
Sbjct: 1   MKGITGVVKKALGEMEFTAAGGAINWFPGHMAAATRAIRDRVKLADMVIEVRDSRIPLSS 60

Query: 82  ANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL 141
           ANQDLQ HLSSKRRIIALNKKDLANPNIMHKW HFF+SCNQDCIPINAHS SSVRKLLEL
Sbjct: 61  ANQDLQSHLSSKRRIIALNKKDLANPNIMHKWTHFFESCNQDCIPINAHSMSSVRKLLEL 120

Query: 142 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVT 201
           VEFKLKEAISREPTLLVMV+GVPNVGKSALINSIHQIASARFPVQEKMKRA+VGPLPGVT
Sbjct: 121 VEFKLKEAISREPTLLVMVIGVPNVGKSALINSIHQIASARFPVQEKMKRANVGPLPGVT 180

Query: 202 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 261
           QDIAG+KIAHQPSIYVLDTPGVLVPSI+D+ETGLKLALAGSVKD+VVGEERIAQYLLAVL
Sbjct: 181 QDIAGFKIAHQPSIYVLDTPGVLVPSIKDVETGLKLALAGSVKDSVVGEERIAQYLLAVL 240

Query: 262 NTRGTPFHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQ 321
           NTRGTPFHWKQ NNRRMEGI+YE EE HRF+LNDLRP+RR P PNKSDVVY+EDLVT+VQ
Sbjct: 241 NTRGTPFHWKQSNNRRMEGIQYESEEMHRFSLNDLRPKRRPPLPNKSDVVYVEDLVTQVQ 300

Query: 322 RALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 381
           RALYAT S FDGNVEDENDLESLIE+QFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK
Sbjct: 301 RALYATFSGFDGNVEDENDLESLIEVQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGK 360

Query: 382 LGPFILDDVPNVN 395
           LGPFILDDVP+ N
Sbjct: 361 LGPFILDDVPSHN 373

BLAST of Lag0011811 vs. ExPASy TrEMBL
Match: A0A1S3CEC4 (short integuments 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499512 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.1e-181
Identity = 333/371 (89.76%), Postives = 349/371 (94.07%), Query Frame = 0

Query: 22  MRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSS 81
           MRGINGV+KKALG+MEFTAGGGAINWFPGHMAAA RAIR RLKLADMVIEVRDSRIPLSS
Sbjct: 1   MRGINGVMKKALGDMEFTAGGGAINWFPGHMAAAIRAIRHRLKLADMVIEVRDSRIPLSS 60

Query: 82  ANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL 141
           ANQDLQPHLSSKRR+IALNKKDLAN NIM+KW+ FFDSCNQDCIPINAHSKSSVRKLLEL
Sbjct: 61  ANQDLQPHLSSKRRVIALNKKDLANSNIMNKWVDFFDSCNQDCIPINAHSKSSVRKLLEL 120

Query: 142 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVT 201
           VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIAS RFPVQEK KRA VGPLPGVT
Sbjct: 121 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASERFPVQEKRKRATVGPLPGVT 180

Query: 202 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 261
           QDIAGYKIAHQPSIYVLDTPGVLVPSIQD ETGLKLALAGSVKD VVGEERIAQYLLAVL
Sbjct: 181 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDTETGLKLALAGSVKDEVVGEERIAQYLLAVL 240

Query: 262 NTRGTPFHWKQ-LNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEV 321
           N+R TP HW++ LNNRRMEGI+YEPEE HRFNLNDL+P+RRA  PNKSD VY+EDLVTEV
Sbjct: 241 NSRRTPLHWRRHLNNRRMEGIQYEPEERHRFNLNDLQPKRRA-LPNKSDAVYVEDLVTEV 300

Query: 322 QRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAG 381
           Q ALY TLS+FDGNVEDEN LESLIE+QFEALQKAMK+ H AAEARL+VSKKLLTLFRAG
Sbjct: 301 QCALYTTLSEFDGNVEDENGLESLIEVQFEALQKAMKVSHMAAEARLRVSKKLLTLFRAG 360

Query: 382 KLGPFILDDVP 392
           KLG FILDDVP
Sbjct: 361 KLGQFILDDVP 370

BLAST of Lag0011811 vs. ExPASy TrEMBL
Match: A0A5A7ULU0 (Short integuments 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold697G00560 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 7.6e-180
Identity = 333/372 (89.52%), Postives = 349/372 (93.82%), Query Frame = 0

Query: 22  MRGINGVVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSS 81
           MRGINGV+KKALG+MEFTAGGGAINWFPGHMAAA RAIR RLKLADMVIEVRDSRIPLSS
Sbjct: 1   MRGINGVMKKALGDMEFTAGGGAINWFPGHMAAAIRAIRHRLKLADMVIEVRDSRIPLSS 60

Query: 82  ANQDLQPHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL 141
           ANQDLQPHLSSKRR+IALNKKDLAN NIM+KW+ FFDSCNQDCIPINAHSKSSVRKLLEL
Sbjct: 61  ANQDLQPHLSSKRRVIALNKKDLANSNIMNKWVDFFDSCNQDCIPINAHSKSSVRKLLEL 120

Query: 142 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVT 201
           VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIAS RFPVQEK KRA VGPLPGVT
Sbjct: 121 VEFKLKEAISREPTLLVMVVGVPNVGKSALINSIHQIASERFPVQEKRKRATVGPLPGVT 180

Query: 202 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAG-SVKDAVVGEERIAQYLLAV 261
           QDIAGYKIAHQPSIYVLDTPGVLVPSIQD ETGLKLALAG SVKD VVGEERIAQYLLAV
Sbjct: 181 QDIAGYKIAHQPSIYVLDTPGVLVPSIQDTETGLKLALAGRSVKDEVVGEERIAQYLLAV 240

Query: 262 LNTRGTPFHWKQ-LNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTE 321
           LN+R TP HW++ LNNRRMEGI+YEPEE HRFNLNDL+P+RRA  PNKSD VY+EDLVTE
Sbjct: 241 LNSRRTPLHWRRHLNNRRMEGIQYEPEERHRFNLNDLQPKRRA-LPNKSDAVYVEDLVTE 300

Query: 322 VQRALYATLSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRA 381
           VQ ALY TLS+FDGNVEDEN LESLIE+QFEALQKAMK+ H AAEARL+VSKKLLTLFRA
Sbjct: 301 VQCALYTTLSEFDGNVEDENGLESLIEVQFEALQKAMKVSHMAAEARLRVSKKLLTLFRA 360

Query: 382 GKLGPFILDDVP 392
           GKLG FILDDVP
Sbjct: 361 GKLGQFILDDVP 371

BLAST of Lag0011811 vs. TAIR 10
Match: AT2G41670.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 519.2 bits (1336), Expect = 2.9e-147
Identity = 266/364 (73.08%), Postives = 306/364 (84.07%), Query Frame = 0

Query: 28  VVKKALGEMEFTAGGGAINWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQ 87
           V K  +G M F    G INWFPGHMAAATRAIR+RLKL+D+VIEVRD+RIPLSSAN+DLQ
Sbjct: 9   VKKGLIGGMSFAKDAGKINWFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQ 68

Query: 88  PHLSSKRRIIALNKKDLANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLELVEFKLK 147
             +S+KRRIIALNKKDLANPN+++KW   F+S  QDCI INAHS+SSV KLL+LVE KLK
Sbjct: 69  SQMSAKRRIIALNKKDLANPNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLK 128

Query: 148 EAISREPTLLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGY 207
           E I+REPTLLVMVVGVPNVGKSALINSIHQIA+ARFPVQE++KRA VGPLPGVTQDIAG+
Sbjct: 129 EVIAREPTLLVMVVGVPNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGF 188

Query: 208 KIAHQPSIYVLDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVLNTRGTP 267
           KIAH+PSIYVLD+PGVLVPSI DIETGLKLAL+GSVKD+VVGEERIAQY LA+LN RGTP
Sbjct: 189 KIAHRPSIYVLDSPGVLVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTP 248

Query: 268 FHWKQLNNRRMEGIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQRALYAT 327
            HWK L     EG   +  +   +NL DLR  +R   P+ S + Y+ D+++EVQR+LY T
Sbjct: 249 LHWKYLVEGINEGPHADCIDKPSYNLKDLR-HQRTKQPDSSALHYVGDMISEVQRSLYIT 308

Query: 328 LSKFDGNVEDENDLESLIELQFEALQKAMKIPHKAAEARLKVSKKLLTLFRAGKLGPFIL 387
           LS+FDG+ EDENDLE LIE QFE LQKA+KIPHKA+EARL VSKK LTLFR G+LGPFIL
Sbjct: 309 LSEFDGDTEDENDLECLIEQQFEVLQKALKIPHKASEARLMVSKKFLTLFRTGRLGPFIL 368

Query: 388 DDVP 392
           DDVP
Sbjct: 369 DDVP 371

BLAST of Lag0011811 vs. TAIR 10
Match: AT4G10650.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.7e-60
Identity = 138/352 (39.20%), Postives = 212/352 (60.23%), Query Frame = 0

Query: 47  WFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQPH--LSSKRRIIALNKKDL 106
           W+  HMAAA RAI +R+ L D V+E+RD+RIPLSS  + L+    L SK RII LNK +L
Sbjct: 28  WYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYELLRKFSPLPSK-RIIVLNKMEL 87

Query: 107 ANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLELVEFKLKE---AISREPTLLVMVV 166
           A+P  + K + +F+  N     +N+H+K  V++LL  ++ +++E   A     T  +M++
Sbjct: 88  ADPLELKKCIDYFEERNYLSYAVNSHNKDCVKQLLNFLQSQVRELHKAGHSGHTTTMMLL 147

Query: 167 GVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIYVLDTP 226
           G+PNVGKSAL NS+H I       + K+K   V   PG T+DI   KI   P++YVLDTP
Sbjct: 148 GIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGDTKDIMSLKIGSHPNVYVLDTP 207

Query: 227 GVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVLNTRGTPFHWKQL--NNRRME 286
           G+  P++ D E   KLAL G++ D +VGE ++A+  L +LN+      W +L  +    E
Sbjct: 208 GIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTILNSSHEYKKWAKLCKSQDLTE 267

Query: 287 GIKYEPEEGHRFNLNDLRPRRRAPPPNKSDVVYIEDLVTEVQRALYATLSKFDGNVEDEN 346
            +  E  +      +D + +R+    +  D +     V +V+R LY T+S FDGN+EDE 
Sbjct: 268 SLSDESSK------SDAKHKRQYATDHTQDFI-----VYDVRRVLYETISAFDGNLEDEI 327

Query: 347 DLESLIELQFEALQKAMKIPHKAAE-ARLKVSKKLLTLFRAGKLGPFILDDV 391
            + +LIE QF AL+  +++P +A+E A L+V+ K+L L+R G+LG + L+ V
Sbjct: 328 SMGNLIETQFAALRSVLRVPEEASEFADLRVASKILNLYRTGRLGHYTLEHV 367

BLAST of Lag0011811 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 103.2 bits (256), Expect = 4.9e-22
Identity = 67/224 (29.91%), Postives = 112/224 (50.00%), Query Frame = 0

Query: 45  INWFPGHMAAATRAIRDRLKLADMVIEVRDSRIPLSSANQDLQPHLSSKRRIIALNKKDL 104
           + W+PGH+    + +R++LKL D+VIEVRD+RIPLS+ +  +   L +++RI+ LN++D+
Sbjct: 96  VQWYPGHIMKTEKELREQLKLMDVVIEVRDARIPLSTTHPKMDAWLGNRKRILVLNREDM 155

Query: 105 ANPNIMHKWMHFFDSCNQDCIPINAHSKSSVRKLLEL-------VEFKLKEAISREPTLL 164
            + +  + W  +F       I  N        KL  L       V  K +E      ++ 
Sbjct: 156 ISNDDRNDWARYFAKQGIKVIFTNGKLGMGAMKLGRLAKSLAGDVNGKRREKGLLPRSVR 215

Query: 165 VMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAHVGPLPGVTQDIAGYKIAHQPSIYV 224
             ++G PNVGKS+LIN +            K K     P PGVT+++   K+     + +
Sbjct: 216 AGIIGYPNVGKSSLINRL-----------LKRKICAAAPRPGVTREMKWVKLG--KDLDL 275

Query: 225 LDTPGVLVPSIQDIETGLKLALAGSVKDAVVGEERIAQYLLAVL 262
           LD+PG+L   I D    +KLA+   + +       +A  L+ +L
Sbjct: 276 LDSPGMLPMRIDDQAAAIKLAICDDIGEKAYDFTDVAGILVQML 306

BLAST of Lag0011811 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.5e-07
Identity = 51/193 (26.42%), Postives = 90/193 (46.63%), Query Frame = 0

Query: 63  LKLADMVIEVRDSRIPLSSANQDLQPHL----SSKRRIIALNKKDLANPNIMHKWMHF-- 122
           ++L+D+++EV D+R PL +   D++  +     +K  ++ LNK DL       KW+ +  
Sbjct: 134 IELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLLNKIDLVPREAAEKWLMYLR 193

Query: 123 -------FDSCNQDCIPINAHSKSSVRKLLELVE----------FKLKEAISR----EPT 182
                  F    Q+         S   K   +++           KL +  SR    + +
Sbjct: 194 EEFPAVAFKCSTQEQRSNLGWKSSKASKPSNMLQTSDCLGADTLIKLLKNYSRSHELKKS 253

Query: 183 LLVMVVGVPNVGKSALINSIHQIASARFPVQEKMKRAH---VGPLPGVTQDIAGYKIAHQ 226
           + V ++G+PNVGKS+LINS              +KRAH   VG  PG+T+ +   ++   
Sbjct: 254 ITVGIIGLPNVGKSSLINS--------------LKRAHVVNVGATPGLTRSL--QEVHLD 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022966362.11.7e-19792.01short integuments 2, mitochondrial [Cucurbita maxima][more]
XP_038883550.13.5e-19594.59short integuments 2, mitochondrial isoform X1 [Benincasa hispida][more]
XP_022156767.17.7e-19591.17short integuments 2, mitochondrial isoform X1 [Momordica charantia] >XP_02215676... [more]
KAG6595673.12.1e-19292.49Short integuments 2, mitochondrial, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022924906.16.1e-19292.23short integuments 2, mitochondrial [Cucurbita moschata] >XP_022924907.1 short in... [more]
Match NameE-valueIdentityDescription
Q8L6074.0e-14673.08Short integuments 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SIN2 PE=1 ... [more]
O824973.7e-5939.20DAR GTPase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DGP2 PE=3 SV=1[more]
Q8R2R61.5e-3639.60Mitochondrial ribosome-associated GTPase 1 OS=Mus musculus OX=10090 GN=Mtg1 PE=1... [more]
Q9BT172.4e-3440.79Mitochondrial ribosome-associated GTPase 1 OS=Homo sapiens OX=9606 GN=MTG1 PE=1 ... [more]
B7GGD63.2e-3436.89Ribosome biogenesis GTPase A OS=Anoxybacillus flavithermus (strain DSM 21510 / W... [more]
Match NameE-valueIdentityDescription
A0A6J1HMR98.0e-19892.01short integuments 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111466034 P... [more]
A0A6J1DRI93.7e-19591.17short integuments 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1EAB73.0e-19292.23short integuments 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111432314... [more]
A0A1S3CEC43.1e-18189.76short integuments 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7ULU07.6e-18089.52Short integuments 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold697G... [more]
Match NameE-valueIdentityDescription
AT2G41670.12.9e-14773.08P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G10650.12.7e-6039.20P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G02790.14.9e-2229.91GTP-binding family protein [more]
AT3G07050.11.5e-0726.42GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 158..264
e-value: 5.3E-14
score: 52.3
IPR023179GTP-binding protein, orthogonal bundle domain superfamilyGENE3D1.10.1580.10coord: 229..381
e-value: 5.7E-6
score: 28.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 45..224
e-value: 2.6E-38
score: 133.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 51..238
NoneNo IPR availablePIRSRPIRSR006230-1PIRSR006230-1coord: 44..264
e-value: 2.3E-58
score: 195.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR45782MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1coord: 44..394
NoneNo IPR availablePANTHERPTHR45782:SF7SHORT INTEGUMENTS 2, MITOCHONDRIAL-LIKEcoord: 44..394
NoneNo IPR availableCDDcd01856YlqFcoord: 47..224
e-value: 2.68157E-68
score: 211.618
IPR030378Circularly permuted (CP)-type guanine nucleotide-binding (G) domainPROSITEPS51721G_CPcoord: 56..226
score: 34.31889

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011811.1Lag0011811.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000911 cytokinesis by cell plate formation
biological_process GO:0048481 plant ovule development
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding