Lag0011611 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011611
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncysteine proteinase inhibitor 5-like
Locationchr1: 29016006 .. 29019141 (-)
RNA-Seq ExpressionLag0011611
SyntenyLag0011611
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTGCTCAATGGTGTACCAAAGGTCCAACAGCGACGTTGTTGCCAACGTCTTGAAACTAGAAACATGTGCAAGGCAACGTAGCCTCCTCAAAATTCGAAGCATTGATACGTTTAAATTTCTACACTCGTTGCTGCTGCCATCAAGTGCTTGTGATCTGACGCCTGAAGAGCTTGCTGCGATGGCAGGTGGAGAAGAGAGGCATGGGAGGTTGGCTGGCGATTGCTGCTTCGTCGAGAAAAAAAATGATAAAACTGAAGAAGATGGTGGTGGTATAATTGTGCATTTGTTTAAATTATAAGTTAGGGTTTTGCTAGGCTCCAATTTTCATTTGTTTACAATTTTTCCACAAGAATCAATTAAATCCCTTCCAACCAAATTAAAACTCCAATTAACCCTCCAAAAGTTTCTCAATTAAATTGATGTTTACCGCCTTACACTTGTCTCTTTCTAGTTTTTGTTTTGTTTCTACAATAATGTGTACCTAAATTTCTGTTTCTAATTCAAGGGTGAATTAATTATAGTTGTAATAATTTGTTGAGTCTTTGTACCAAATAGAATTTATTTTATTCAAGATTTGTTTTTCTTTCTTATTCTCTATTTTTTTATTAATAATTTGATATTTAATTAAATTGACAAATTAATTTAATATCGATTGTTCTATATTGTCGTTGATGAATTTGTGATATGTAATTGTCATGAATGATTTAATTTAGCAACAATGAGCTAGTTTGCTTAGGTATGGGTAAGTATAACTTTTCCATTTGACATAACTTGCATAATAAACTCTAGAGGACAATATCTCGATTAATTGAATTTGGTTTTTAGTCATTCCCATATAGATTTATTTTTAATGCTATCATTAAATCGATATTGGACACTATGGTGTCCAATTGATTAAATGATAAGTGTTTTTTTTTTTTTTTTTTTTGGAAAAGTTAAATGATAAGTGTTTATTTTGATGTTTGGTTTAGAATCAAATAAATAGAGAATGTACACACTAATTATGGCTTTACCACTTAAGTAGATTAAGTTCTTGAATTGAGAATTTGATTTTTTTTTTTTTAAGTTTGTGATCAAGATTACAATGGGATAGAATTTGCGCCCTTGATTTCAAATTTGGTAATTTTATTTTTATATTCACTTCTTCATTTCTTAATTTTTATCTTAGTTCAACATTTCAAAACCTTATTTTATTTTAGTTTTCTTAAACCTTGAGAAATTAATTTGACTAGTTCTCTTGAGATACAACTTAGTTTCACATCTCTCCACAAGTTACATTAGAAAGATCAATTATTACTATGCAGCGCCTCGAAAACCAGACCAATATCCATTAATCGTCGATTAACATCTTATTGGAATAAACCTTTGTATTCTTTCATATATATCTACTTAATATCTTTTAATATATTCCAAAATTCTTTCTATCACATTTGGAATCAAGAATCGAAATTTGACTTGGAAAAATCCAACATGAGAACTGTAACCGCCCAAAATTTCAACAAGTTAGAATGTATACTTAATTAAACTTTTATATTCTTCCATATCTAATATTTTATATTCTAAAATATGTGTGCTTATTATTTTTAGTCAACACCAAAGTCAAGAGCTTTAATTAACAAGTTAGAATGTATATTTAATTCTTTCTATCACATTTTAAATGGAGAATCTAAGAATGGGGGAGGAAGAAGAGAGGAAAGAGAGAGAAAAATAAATAAATGAATAAAATGTTTTTAATTAACAAAATAATTTTGAAAAAATAAATTTATTAAAAAAATTGTCACATCAGTTTTCTGTTAGTAATTAACAGAAAAGTTAACGAAGGGACTAGAGGTATAACAAAATCCAAACCTTAGAAGTAAAATTGTTGAATTTGAAACTTTTGGAACCAAAATGTAACCTATCCCTATCCTGATTAAAAGTGTATTTGTCCCTAAATTTTATTGAAACATTTGCGTGGATGACCCACTCGAGAGGTAGAATAAAAAATGTCCTAAATTTTTATTTATTATTTTTTTTTTATTTGACAATGGACATGCCTTTGTTTAGTGAATAGAATCTCATATAAAATACTTAATAAATATCTCAATTCACATTAAACCATCCACATATGTCATAGCCAAATTTTCAACAAGTTAGAATAATCTATACTTAATTAAACTTTTATATTCTTCCATATCTAATATATTTTATGTTCTAAAATATTTGTGCTTATTCTTATTCTTTTTGGGTTGATACCAAATTTAAGAGCTTTAATTAACACATTAGAATGTGTACTTAATTTATTTTAATGTATTCCAAAATTCTTTCTATCACATTTGGAGTCGAGAATCTAAATTTGACTTAGAAAAATCCAACATGAAAACTGTAACGGTCCAAAATTTCAACAAGTTAGAATAATCTATACTTGATTAAACCTTTATATTCTCCCATATCTAATAATCTATGCTTTAGCATATCTGCCTTTCATTTTAGTTTCACTAAATTACATGGTAAATTAATTAGTTGTCATGAAGAATATTTGAAAATGAAGACATTAGAAGAATGTTGTTGAGTAAACAAATTTTATTCGAAACTATCTTTTAATTAGCTATGAAACTCGTATTGGTCCACTGTTTTTTATGAAATCTGCATATTAAGATTCCAGGTATTAAGTTACTATTGAACGACTCTTAACTCCACAAATAATACTGTTGGACGATATTCATTGCTTGGGATCAAACACAATTCTATAAATATCAAACTACCATTTTAGGTGAAGAGCAAACGACCCGCTTACAAAATTTTGTTTAGGTGGAAACTATCAATCAAAAGTTAGGATGAGTTCTTCTGGACAAGATGATTGGAAACAAATAGAGAACATTAACGACGATCCGTATGTGCAAGAGATCGCAAAGTTTGCAGTGACGAAGAGAACACACGATATACTTGGGGCATTGACATTCATTGGAGTGGTGAGTGGTGAGGTTGCAAAGGTGAATGAAGGAGAAAAGTACCGGCTTAAGGTAGAGGTGAAAATAGAGATAGTATCTAACAATCCTCCCCTACCAAAATATATAAAAGTTGTTGTGCTGGATCGTAAACCACCACCAATTGAATCTTGGGAACTCTTAGATTTTGATTTTCCCAATTAG

mRNA sequence

ATGCATTGCTCAATGGTGTACCAAAGGTCCAACAGCGACGTTGTTGCCAACGTCTTGAAACTAGAAACATGTGCAAGGCAACGTAGCCTCCTCAAAATTCGAAGCATTGATACGTTTAAATTTCTACACTCGTTGCTGCTGCCATCAAGTGCTTGTGATCTGACGCCTGAAGAGCTTGCTGCGATGGCAGGTGGAGAAGAGAGGCATGGGAGGTTGGCTGGCGATTGCTGCTTCGTCGAGAAAAAAAATGATAAAACTGAAGAAGATGGTGGTGGTGGAAACTATCAATCAAAAGTTAGGATGAGTTCTTCTGGACAAGATGATTGGAAACAAATAGAGAACATTAACGACGATCCGTATGTGCAAGAGATCGCAAAGTTTGCAGTGACGAAGAGAACACACGATATACTTGGGGCATTGACATTCATTGGAGTGGTGAGTGGTGAGGTTGCAAAGGTGAATGAAGGAGAAAAGTACCGGCTTAAGGTAGAGGTGAAAATAGAGATAGTATCTAACAATCCTCCCCTACCAAAATATATAAAAGTTGTTGTGCTGGATCGTAAACCACCACCAATTGAATCTTGGGAACTCTTAGATTTTGATTTTCCCAATTAG

Coding sequence (CDS)

ATGCATTGCTCAATGGTGTACCAAAGGTCCAACAGCGACGTTGTTGCCAACGTCTTGAAACTAGAAACATGTGCAAGGCAACGTAGCCTCCTCAAAATTCGAAGCATTGATACGTTTAAATTTCTACACTCGTTGCTGCTGCCATCAAGTGCTTGTGATCTGACGCCTGAAGAGCTTGCTGCGATGGCAGGTGGAGAAGAGAGGCATGGGAGGTTGGCTGGCGATTGCTGCTTCGTCGAGAAAAAAAATGATAAAACTGAAGAAGATGGTGGTGGTGGAAACTATCAATCAAAAGTTAGGATGAGTTCTTCTGGACAAGATGATTGGAAACAAATAGAGAACATTAACGACGATCCGTATGTGCAAGAGATCGCAAAGTTTGCAGTGACGAAGAGAACACACGATATACTTGGGGCATTGACATTCATTGGAGTGGTGAGTGGTGAGGTTGCAAAGGTGAATGAAGGAGAAAAGTACCGGCTTAAGGTAGAGGTGAAAATAGAGATAGTATCTAACAATCCTCCCCTACCAAAATATATAAAAGTTGTTGTGCTGGATCGTAAACCACCACCAATTGAATCTTGGGAACTCTTAGATTTTGATTTTCCCAATTAG

Protein sequence

MHCSMVYQRSNSDVVANVLKLETCARQRSLLKIRSIDTFKFLHSLLLPSSACDLTPEELAAMAGGEERHGRLAGDCCFVEKKNDKTEEDGGGGNYQSKVRMSSSGQDDWKQIENINDDPYVQEIAKFAVTKRTHDILGALTFIGVVSGEVAKVNEGEKYRLKVEVKIEIVSNNPPLPKYIKVVVLDRKPPPIESWELLDFDFPN
Homology
[Close]
BLAST of Lag0011611 vs. NCBI nr
Match: XP_038882290.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 75.9 bits (185), Expect = 4.6e-10
Identity = 43/94 (45.74%), Postives = 64/94 (68.09%), Query Frame = 0 

Query: 109 WKQIENINDDPYVQEIAKFAVTKRTHDILGA-LTFIGVVSGEVAKVNEGEKYRLKVEVKI 168
           W+ I+NI+ DPYVQE+ +FAV + TH ++GA L FI VVSGE   +++G++YRL VEV  
Sbjct: 24  WEPIKNIH-DPYVQEMGRFAVMEHTHKLVGASLKFICVVSGETRPMSQGKEYRLVVEVAE 83

Query: 169 EIVSNNPPLPKYIKVVVLDRKPPPIE-SWELLDF 201
           +I+++ P L   IK  +      PI+ SW+L+ F
Sbjct: 84  QIITSPPMLSVSIKFYLATVLEKPIDRSWQLISF 116

[Close]
BLAST of Lag0011611 vs. NCBI nr
Match: KAG6603238.1 (Cysteine proteinase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 73.2 bits (178), Expect = 3.0e-09
Identity = 51/109 (46.79%), Postives = 64/109 (58.72%), Query Frame = 0 

Query: 96  QSKVRMSSSGQDDWKQIENINDDPYVQEIAKFAVTKRTHDILG-ALTFIGVVSGEVAKVN 155
           Q  V + +    DWK IE+I  DPYVQEI +FA+TK  H   G   TFI VV+GE   V 
Sbjct: 5   QDDVGLQACKPHDWKSIEDIK-DPYVQEIGRFAMTKFIHHQTGKTWTFIRVVNGETLAVE 64

Query: 156 EGEKYRLKVEVKIEIV--SNNPPLPKYIKVVVLDRKPPPIESWELLDFD 202
           EG  YR+ +EVK +IV  S    +  Y+   VLD K P  +SWELL F+
Sbjct: 65  EGMLYRVVIEVKTDIVVQSYLGSIELYV-AKVLDHKQPR-KSWELLAFE 110

[Close]
BLAST of Lag0011611 vs. NCBI nr
Match: WP_198843152.1 (hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical protein [Aureibaculum sp. A20])

HSP 1 Score: 69.3 bits (168), Expect = 4.3e-08
Identity = 40/94 (42.55%), Postives = 61/94 (64.89%), Query Frame = 0 

Query: 109 WKQIENINDDPYVQEIAKFAVTKRTHDILGA-LTFIGVVSGEVAKVNEGEKYRLKVEVKI 168
           W+ I+NI+ DPYVQE+ +F+V + TH ++GA + FI VVSGE   ++EG++YRL VEV  
Sbjct: 28  WEPIKNIH-DPYVQEMGRFSVMEHTHKLVGASMKFIRVVSGETRPMSEGKEYRLVVEVAE 87

Query: 169 EIVSNNPPLPKYIKVVVLDRKPPPIE-SWELLDF 201
            I++  P L   +K  +      P++ SW+L  F
Sbjct: 88  HIITCPPMLSVSVKFYLATVLEKPLDRSWQLEAF 120

[Close]
BLAST of Lag0011611 vs. NCBI nr
Match: KAG6590493.1 (hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 69.3 bits (168), Expect = 4.3e-08
Identity = 45/107 (42.06%), Postives = 66/107 (61.68%), Query Frame = 0 

Query: 96  QSKVRMSSSGQDDWKQIENINDDPYVQEIAKFAVTKRTHDILGA-LTFIGVVSGEVAKVN 155
           Q  V   +  Q DW+ I+N  DDPYVQE+ +FAV +  H ++GA  +F+GVVS E   V 
Sbjct: 10  QDGVLNQNHKQCDWELIKN-KDDPYVQEMGRFAVMEHVHGLVGAKWSFVGVVSCESLVVP 69

Query: 156 EGEKYRLKVEVKIEI-VSNNPPLPKYIKVVVLDRKPPPIESWELLDF 201
           +G+ YRL ++V IEI V  +P   +  + +VLD++     SWEL+ F
Sbjct: 70  KGKLYRLLIKVDIEIVVPCHPHAIEVYEAIVLDKECE--RSWELMSF 113

[Close]
BLAST of Lag0011611 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 68.9 bits (167), Expect = 5.6e-08
Identity = 44/94 (46.81%), Postives = 62/94 (65.96%), Query Frame = 0 

Query: 109 WKQIENINDDPYVQEIAKFAVTKRTHDILGA-LTFIGVVSGEVAKVNEGEKYRLKVEVK- 168
           W+ I+NIN DPYVQE+ +FAV + TH++ GA   FI VVSGE ++V  G+KY L +EVK 
Sbjct: 25  WEPIKNIN-DPYVQELGRFAVMEHTHNLCGASYEFIRVVSGE-SQVVGGKKYCLVIEVKE 84

Query: 169 IEIVSNNPPLPKYIKVVVLDRKPPPIESWELLDF 201
             I+ + P   K+ K ++LD+     +SW LL F
Sbjct: 85  TIIIPSMPSSIKFFKAIMLDKAWE--KSWALLSF 114

[Close]
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.7e-08
Identity = 44/94 (46.81%), Postives = 62/94 (65.96%), Query Frame = 0 

Query: 109 WKQIENINDDPYVQEIAKFAVTKRTHDILGA-LTFIGVVSGEVAKVNEGEKYRLKVEVK- 168
           W+ I+NIN DPYVQE+ +FAV + TH++ GA   FI VVSGE ++V  G+KY L +EVK 
Sbjct: 25  WEPIKNIN-DPYVQELGRFAVMEHTHNLCGASYEFIRVVSGE-SQVVGGKKYCLVIEVKE 84

Query: 169 IEIVSNNPPLPKYIKVVVLDRKPPPIESWELLDF 201
             I+ + P   K+ K ++LD+     +SW LL F
Sbjct: 85  TIIIPSMPSSIKFFKAIMLDKAWE--KSWALLSF 114

[Close]
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match: A0A6J1BUK6 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005500 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-07
Identity = 40/99 (40.40%), Postives = 65/99 (65.66%), Query Frame = 0 

Query: 109 WKQIENINDDPYVQEIAKFAVTK---RTHDILGALTFIGVVSGEVAKVNEGEKYRLKVEV 168
           W+ I+NI  DPYVQE+ +FAV +   +  +++ ++ FIGVVSGE+  V  G++YRL VEV
Sbjct: 23  WEPIKNIK-DPYVQEMGRFAVMEQHPKPSELIASMEFIGVVSGEIQLVAHGKEYRLVVEV 82

Query: 169 KI--EIVSNNPPLP--KYIKVVVLDRKPPPIESWELLDF 201
           ++  +I+ +  P+   +    VVLD+  P  +SW++L F
Sbjct: 83  RVTEKIIVHGCPVSSIRLYVAVVLDK--PSDKSWKVLSF 118

[Close]
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match: A0A6J1BX23 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005501 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.0e-07
Identity = 40/98 (40.82%), Postives = 64/98 (65.31%), Query Frame = 0 

Query: 109 WKQIENINDDPYVQEIAKFAVTKRTHDI--LGALTFIGVVSGEVAKVNEGEKYRLKVEVK 168
           W+ I+NI  DPYVQE+ +FAV ++   +  + ++ F+GVVSGE+  V  G++YRL VEVK
Sbjct: 54  WEPIKNIK-DPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVEVK 113

Query: 169 I--EIVSNNPPLP--KYIKVVVLDRKPPPIESWELLDF 201
           +  +I+ +  P+   +    VVLD+  P  +SW++L F
Sbjct: 114 VTEKIIVHGCPVSSIRLYVAVVLDK--PSDKSWKVLSF 148

[Close]
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match: A0A5A7UB42 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold48614G00010 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.9e-07
Identity = 43/108 (39.81%), Postives = 62/108 (57.41%), Query Frame = 0 

Query: 96  QSKVRMSSSGQDDWKQIENINDDPYVQEIAKFAVTK--RTHDILGALTFIGVVSGEVAKV 155
           ++ + + +    +WK IE+   DPY++++ +F+V K  R  DI    TFI VVS E   V
Sbjct: 10  EANLLVQNHKNSEWKAIED-TKDPYIEDMGRFSVAKFTRNQDIEEMATFIRVVSSEYLVV 69

Query: 156 NEGEKYRLKVEVKIEIVSNNPPLPKYIKVVVLDRKPPPIESWELLDFD 202
           NEG  YR+ VE++ E VS +  L  Y+   VLD K  P  SW LL F+
Sbjct: 70  NEGTMYRIVVEIRKENVSQSREL--YV-AKVLDHK-HPFNSWTLLSFE 112

[Close]
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.5e-06
Identity = 42/95 (44.21%), Postives = 59/95 (62.11%), Query Frame = 0 

Query: 108 DWKQIENINDDPYVQEIAKFAVTKRTHDILG-ALTFIGVVSGEVAKVNEGEKYRLKVEVK 167
           DW+ + N++D  YVQE+ +FAV +  H + G  +TFI VVSGE   V EG+KY L VEVK
Sbjct: 22  DWEPV-NVHDQ-YVQELGRFAVMEHVHGLCGVGMTFICVVSGESKMVCEGKKYSLVVEVK 81

Query: 168 -IEIVSNNPPLPKYIKVVVLDRKPPPIESWELLDF 201
            + ++ +  P  K     VLD++    +SWELL F
Sbjct: 82  EVPVLPHACPAIKLYVADVLDKQ--CAKSWELLAF 112

The following BLAST results are available for this feature:
BLAST of Lag0011611 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038882290.14.6e-1045.74cysteine proteinase inhibitor 5-like [Benincasa hispida][more]
KAG6603238.13.0e-0946.79Cysteine proteinase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia][more]
WP_198843152.14.3e-0842.55hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical ... [more]
KAG6590493.14.3e-0842.06hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022132557.15.6e-0846.81cysteine proteinase inhibitor 6-like [Momordica charantia][more]
BLAST of Lag0011611 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BU612.7e-0846.81cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1BUK61.8e-0740.40cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1BX233.0e-0740.82cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A5A7UB423.9e-0739.81Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1GCT21.5e-0644.21uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC1114530... [more]
BLAST of Lag0011611 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 113..168
e-value: 1.1E-7
score: 32.1
NoneNo IPR availableGENE3D3.10.450.10coord: 103..202
e-value: 1.1E-10
score: 43.6
NoneNo IPR availablePANTHERPTHR47364CYSTEINE PROTEINASE INHIBITOR 5coord: 101..200
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 112..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011611.1Lag0011611.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity