Lag0011393 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011393
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionE3 ubiquitin-protein ligase MBR2-like
Locationchr1: 23602834 .. 23603553 (-)
RNA-Seq ExpressionLag0011393
SyntenyLag0011393
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTGCCTCCGATACTGTATCCGCGCCACTCCAAACACCATGCCTTTTGCAAATTGTTCCACCGTCCGGTACTGCCTCCGCCGCAACGACGGCGCCGATGAACAAGTGCTCCAAGTCCGGGTGGGTTACGTGAGCCGTCTGATCTCCCGGTCGCCGTCGGGCGGACTTCCGTACGACATCATCGGAGAAAGTCCGTGGAGTCCAGTGGCGGATGGGGAGTTCCCTCTCCCTCTTCGAGAGCTGGAAGATCCGTTGTTGTGCGAGGCGTACGTGGTTGAACTGCTGCTGTCGTGGTTCAAGCTGGAGCGTGCAACCTGCAACGTCATGGCTCGGAAGGTGACGTCGTTGGTGGGTGAGATCTTGGGCGAAGGAAATTACGACGTGAAGTTCCAGGCGGTGGCGGATATCGAATACATTCATATGTTCTGGGCGGAGGAGCGGAGGAGGAGGAGCTCGGAGAATGTTAATGTTAATGTTAATGTTGGGCGGCGTGGTCATAGTCATGGTGTAATCATGGAGGAGAAAGTGGTTTTGGTGGCGAGGGAGATTAAGCGGGAACACTCGATCAAAGAATGCAGCATCTGTTGCGATGAACTACTCAACGACGTGGCTAAAATCCCATGCGGCCATGTTTATCACAAATCCTGCATCCTCACCTGGTTTCACATTAGCAATTCATGTCCCTTGTGTAGAACCAAAATCAAACAACAAACTTAA

mRNA sequence

ATGTCTTGCCTCCGATACTGTATCCGCGCCACTCCAAACACCATGCCTTTTGCAAATTGTTCCACCGTCCGGTACTGCCTCCGCCGCAACGACGGCGCCGATGAACAAGTGCTCCAAGTCCGGGTGGGTTACGTGAGCCGTCTGATCTCCCGGTCGCCGTCGGGCGGACTTCCGTACGACATCATCGGAGAAAGTCCGTGGAGTCCAGTGGCGGATGGGGAGTTCCCTCTCCCTCTTCGAGAGCTGGAAGATCCGTTGTTGTGCGAGGCGTACGTGGTTGAACTGCTGCTGTCGTGGTTCAAGCTGGAGCGTGCAACCTGCAACGTCATGGCTCGGAAGGTGACGTCGTTGGTGGGTGAGATCTTGGGCGAAGGAAATTACGACGTGAAGTTCCAGGCGGTGGCGGATATCGAATACATTCATATGTTCTGGGCGGAGGAGCGGAGGAGGAGGAGCTCGGAGAATGTTAATGTTAATGTTAATGTTGGGCGGCGTGGTCATAGTCATGGTGTAATCATGGAGGAGAAAGTGGTTTTGGTGGCGAGGGAGATTAAGCGGGAACACTCGATCAAAGAATGCAGCATCTGTTGCGATGAACTACTCAACGACGTGGCTAAAATCCCATGCGGCCATGTTTATCACAAATCCTGCATCCTCACCTGGTTTCACATTAGCAATTCATGTCCCTTGTGTAGAACCAAAATCAAACAACAAACTTAA

Coding sequence (CDS)

ATGTCTTGCCTCCGATACTGTATCCGCGCCACTCCAAACACCATGCCTTTTGCAAATTGTTCCACCGTCCGGTACTGCCTCCGCCGCAACGACGGCGCCGATGAACAAGTGCTCCAAGTCCGGGTGGGTTACGTGAGCCGTCTGATCTCCCGGTCGCCGTCGGGCGGACTTCCGTACGACATCATCGGAGAAAGTCCGTGGAGTCCAGTGGCGGATGGGGAGTTCCCTCTCCCTCTTCGAGAGCTGGAAGATCCGTTGTTGTGCGAGGCGTACGTGGTTGAACTGCTGCTGTCGTGGTTCAAGCTGGAGCGTGCAACCTGCAACGTCATGGCTCGGAAGGTGACGTCGTTGGTGGGTGAGATCTTGGGCGAAGGAAATTACGACGTGAAGTTCCAGGCGGTGGCGGATATCGAATACATTCATATGTTCTGGGCGGAGGAGCGGAGGAGGAGGAGCTCGGAGAATGTTAATGTTAATGTTAATGTTGGGCGGCGTGGTCATAGTCATGGTGTAATCATGGAGGAGAAAGTGGTTTTGGTGGCGAGGGAGATTAAGCGGGAACACTCGATCAAAGAATGCAGCATCTGTTGCGATGAACTACTCAACGACGTGGCTAAAATCCCATGCGGCCATGTTTATCACAAATCCTGCATCCTCACCTGGTTTCACATTAGCAATTCATGTCCCTTGTGTAGAACCAAAATCAAACAACAAACTTAA

Protein sequence

MSCLRYCIRATPNTMPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGEFPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAVADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREIKREHSIKECSICCDELLNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKIKQQT
Homology
BLAST of Lag0011393 vs. NCBI nr
Match: XP_022947841.1 (NEP1-interacting protein-like 1 [Cucurbita moschata])

HSP 1 Score: 227.3 bits (578), Expect = 1.4e-55
Identity = 128/231 (55.41%), Postives = 156/231 (67.53%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           MPFAN ST+RY LRRN GAD Q+LQ+R+GY SRLISRSPS GLPYDI+ E+P +P+AD  
Sbjct: 1   MPFANASTLRYSLRRNHGAD-QLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S F L   T N + +K+TS V +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-FNLGSVTWNPIVQKITSFVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVL----VAREIKREHSI 194
           ADI+YI MFW E+ R           +VGR     GV++EE+VVL    VA   K+    
Sbjct: 121 ADIDYIQMFWMEKHRG----------SVGR-----GVMVEERVVLEETGVAAAEKQGIGG 180

Query: 195 KECSICCDEL------LNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +ECS+C +          +VAKIPCGH +HKSCILTWF  SNSCPLCR K+
Sbjct: 181 EECSVCYESYEAEKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Lag0011393 vs. NCBI nr
Match: KAG6605222.1 (E3 ubiquitin-protein ligase AMFR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 227.3 bits (578), Expect = 1.4e-55
Identity = 128/231 (55.41%), Postives = 156/231 (67.53%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           MPFAN ST+RY LRRN GAD Q+LQ+R+GY SRLISRSPS GLPYDI+ E+P +P+AD  
Sbjct: 1   MPFANASTLRYSLRRNHGAD-QLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S F L   T N + +K+TS V +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-FNLGSVTWNPIVQKITSFVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVL----VAREIKREHSI 194
           ADI+YI MFW E+R            +VGR     GV++EE+VVL    VA   K+    
Sbjct: 121 ADIDYIQMFWMEKR----------GGSVGR-----GVMVEERVVLEETGVAAAEKQGIGG 180

Query: 195 KECSICCDEL------LNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +ECS+C +          +VAKIPCGH +HKSCILTWF  SNSCPLCR K+
Sbjct: 181 EECSVCYESYEAEKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Lag0011393 vs. NCBI nr
Match: XP_023006825.1 (NEP1-interacting protein-like 1 [Cucurbita maxima])

HSP 1 Score: 223.4 bits (568), Expect = 2.1e-54
Identity = 125/231 (54.11%), Postives = 155/231 (67.10%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           MP AN ST+RY LRRN GAD Q L+ R+ Y SRLISRSPS GLPYDI+ E+P +P+AD  
Sbjct: 1   MPLANASTLRYSLRRNHGAD-QFLRFRLCYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY++EL+ S   L+  +CN + +K+TSLV +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLIELVSS-LNLDSMSCNPIVQKITSLVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREI----KREHSI 194
           ADI+YI MFW E+R            +VGR     GV++EEKVVL   E+    K+    
Sbjct: 121 ADIDYIQMFWMEKRPG----------SVGR-----GVMVEEKVVLKEAEVAAAEKQGIGG 180

Query: 195 KECSICCDEL------LNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +ECS+C +          +VAKIPCGHV+HKSCILTWF  SNSCPLCR K+
Sbjct: 181 EECSVCYESYEAEKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Lag0011393 vs. NCBI nr
Match: KAG6605180.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.5 bits (558), Expect = 3.0e-53
Identity = 123/227 (54.19%), Postives = 149/227 (65.64%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           M FAN ST+RY LRR DG D Q+LQVR+GY SRLISRSP  GLPYDI+ E P +P+AD  
Sbjct: 1   MLFANSSTLRYSLRRKDGTD-QLLQVRLGYSSRLISRSPYSGLPYDILEEIPSTPIADTL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S   L+  +CN +A+K+TSLV +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-LNLDSISCNPIAQKITSLVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREIKREHSIKECS 194
           ADI+YI MFW EE R      V V   V        ++ E  V   +   K+    +ECS
Sbjct: 121 ADIDYIQMFWREESRGPVRRGVMVEEKV--------ILEEAGVAATSAAGKQGMGGEECS 180

Query: 195 I---CCDELL---NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +   C DE      +VAKIPCGH++HKSCILTWF  SNSCPLCR K+
Sbjct: 181 VCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFQCSNSCPLCRAKL 216

BLAST of Lag0011393 vs. NCBI nr
Match: XP_022947853.1 (E3 ubiquitin-protein ligase MBR2-like [Cucurbita moschata])

HSP 1 Score: 216.5 bits (550), Expect = 2.6e-52
Identity = 123/227 (54.19%), Postives = 150/227 (66.08%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           M FAN ST+RY LRR DG D Q+LQVR+GY SRLISRSP  GLPYDI+ E+P + +AD  
Sbjct: 1   MLFANSSTLRYSLRRKDGTD-QLLQVRLGYSSRLISRSPYSGLPYDILEETPSTLIADTL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S   L+  +CN +A+K+TSLV +     +YD+KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-LNLDSISCNPIAQKITSLVTQ-WAAVDYDLKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREIKREHSIKECS 194
           ADI+YI MFW EE R      V V   V        V+ E  V   +   K+    +ECS
Sbjct: 121 ADIDYIQMFWREESRGPVRRGVMVEEKV--------VLEEAGVAATSAAGKQGMGGEECS 180

Query: 195 I---CCDELL---NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +   C DE      +VAKIPCGH++HKSCILTWF  SNSCPLCR K+
Sbjct: 181 VCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFLCSNSCPLCRAKL 216

BLAST of Lag0011393 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-07
Identity = 24/61 (39.34%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 177 VVLVAREIKREHSIKECSICCDELLND--VAKIPCGHVYHKSCILTWFHISNSCPLCRTK 236
           V + A E+ R+    +CSIC D+   D  V K+PC H+YH++CI+ W ++ ++CP+CR  
Sbjct: 240 VQINAEEVNRK---IQCSICWDDFKIDETVRKLPCSHLYHENCIVPWLNLHSTCPICRKS 297

BLAST of Lag0011393 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-07
Identity = 20/48 (41.67%), Postives = 34/48 (70.83%), Query Frame = 0

Query: 190 IKECSICCDELL--NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           + +C++C DE    +DV ++PC HV+H+ C+L W  + NSCP+CR ++
Sbjct: 213 MNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of Lag0011393 vs. ExPASy Swiss-Prot
Match: Q95SP2 (E3 ubiquitin-protein ligase HRD1 OS=Drosophila melanogaster OX=7227 GN=sip3 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.6e-07
Identity = 20/53 (37.74%), Postives = 35/53 (66.04%), Query Frame = 0

Query: 183 EIKREHSIKECSICCDELLNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           E+++  +I  C IC ++++N   K+PCGH++H +C+ +WF    +CP CR  I
Sbjct: 281 ELRQSDNI--CIICREDMVNHSKKLPCGHIFHTTCLRSWFQRQQTCPTCRLNI 331

BLAST of Lag0011393 vs. ExPASy Swiss-Prot
Match: Q75DC2 (Alcohol-sensitive RING finger protein 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=ASR1 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.4e-07
Identity = 23/47 (48.94%), Postives = 33/47 (70.21%), Query Frame = 0

Query: 192 ECSICCDELLNDVAKI-PCGHVYHKSCILTWFHI---SNSCPLCRTK 235
           ECSIC + + + V ++ PCGH YH +CI  WFH+   + SCP+CRT+
Sbjct: 17  ECSICWESMPSGVGRLMPCGHEYHLACIRKWFHLHSGNRSCPVCRTE 63

BLAST of Lag0011393 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.4e-07
Identity = 26/66 (39.39%), Postives = 39/66 (59.09%), Query Frame = 0

Query: 175 EKVVLVAREIK-REHSIKECSICCDEL-LNDVAKI--PCGHVYHKSCILTWFHISNSCPL 234
           EK+ +    +K  E  ++ECS+C  E   +D  ++   CGHV+H  CI TWF   +SCPL
Sbjct: 94  EKIPIFVYSVKTHESPLEECSVCLSEFEEDDEGRVLPKCGHVFHVDCIDTWFRSRSSCPL 153

Query: 235 CRTKIK 237
           CR  ++
Sbjct: 154 CRAPVQ 159

BLAST of Lag0011393 vs. ExPASy TrEMBL
Match: A0A6J1G7L1 (NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 7.0e-56
Identity = 128/231 (55.41%), Postives = 156/231 (67.53%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           MPFAN ST+RY LRRN GAD Q+LQ+R+GY SRLISRSPS GLPYDI+ E+P +P+AD  
Sbjct: 1   MPFANASTLRYSLRRNHGAD-QLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S F L   T N + +K+TS V +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-FNLGSVTWNPIVQKITSFVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVL----VAREIKREHSI 194
           ADI+YI MFW E+ R           +VGR     GV++EE+VVL    VA   K+    
Sbjct: 121 ADIDYIQMFWMEKHRG----------SVGR-----GVMVEERVVLEETGVAAAEKQGIGG 180

Query: 195 KECSICCDEL------LNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +ECS+C +          +VAKIPCGH +HKSCILTWF  SNSCPLCR K+
Sbjct: 181 EECSVCYESYEAEKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Lag0011393 vs. ExPASy TrEMBL
Match: A0A6J1L186 (NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.0e-54
Identity = 125/231 (54.11%), Postives = 155/231 (67.10%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           MP AN ST+RY LRRN GAD Q L+ R+ Y SRLISRSPS GLPYDI+ E+P +P+AD  
Sbjct: 1   MPLANASTLRYSLRRNHGAD-QFLRFRLCYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY++EL+ S   L+  +CN + +K+TSLV +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLIELVSS-LNLDSMSCNPIVQKITSLVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREI----KREHSI 194
           ADI+YI MFW E+R            +VGR     GV++EEKVVL   E+    K+    
Sbjct: 121 ADIDYIQMFWMEKRPG----------SVGR-----GVMVEEKVVLKEAEVAAAEKQGIGG 180

Query: 195 KECSICCDEL------LNDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +ECS+C +          +VAKIPCGHV+HKSCILTWF  SNSCPLCR K+
Sbjct: 181 EECSVCYESYEAEKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Lag0011393 vs. ExPASy TrEMBL
Match: A0A6J1G7R8 (E3 ubiquitin-protein ligase MBR2-like OS=Cucurbita moschata OX=3662 GN=LOC111451612 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 1.2e-52
Identity = 123/227 (54.19%), Postives = 150/227 (66.08%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           M FAN ST+RY LRR DG D Q+LQVR+GY SRLISRSP  GLPYDI+ E+P + +AD  
Sbjct: 1   MLFANSSTLRYSLRRKDGTD-QLLQVRLGYSSRLISRSPYSGLPYDILEETPSTLIADTL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S   L+  +CN +A+K+TSLV +     +YD+KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-LNLDSISCNPIAQKITSLVTQ-WAAVDYDLKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREIKREHSIKECS 194
           ADI+YI MFW EE R      V V   V        V+ E  V   +   K+    +ECS
Sbjct: 121 ADIDYIQMFWREESRGPVRRGVMVEEKV--------VLEEAGVAATSAAGKQGMGGEECS 180

Query: 195 I---CCDELL---NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           +   C DE      +VAKIPCGH++HKSCILTWF  SNSCPLCR K+
Sbjct: 181 VCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFLCSNSCPLCRAKL 216

BLAST of Lag0011393 vs. ExPASy TrEMBL
Match: A0A6J1L191 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111499501 PE=4 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 3.6e-52
Identity = 124/237 (52.32%), Postives = 154/237 (64.98%), Query Frame = 0

Query: 15  MPFANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSPSGGLPYDIIGESPWSPVADGE 74
           M FAN ST+RY LRR+ G D Q+LQVR+GY SRLIS+SPS GLPYDI+ E+P +P+AD  
Sbjct: 1   MLFANSSTLRYSLRRHHGTD-QLLQVRLGYSSRLISQSPSAGLPYDILEETPLTPIADAL 60

Query: 75  FPLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAV 134
           FPLPLRELEDPL C AY+VEL+ S   L+  +CN + +K+TSLV +     +YD KFQ V
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSS-LNLDSMSCNPIIQKITSLVTQ-WAAVDYDAKFQLV 120

Query: 135 ADIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVL----------VAREI 194
            DI+YI MFW EE R              RR    GV++EE++VL           A   
Sbjct: 121 VDIDYIQMFWREESR-----------GPVRR----GVMVEERIVLDKAGVAATVAAAAAG 180

Query: 195 KREHSIKECSI---CCDELL---NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           K+    +ECS+   C DE      +VAKIPCGH++HKSCILTWF  SNSCPLCR K+
Sbjct: 181 KQGMGGEECSVCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFQCSNSCPLCRAKL 219

BLAST of Lag0011393 vs. ExPASy TrEMBL
Match: A0A5D3BYE9 (RING finger protein 165-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00110 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.1e-32
Identity = 84/223 (37.67%), Postives = 132/223 (59.19%), Query Frame = 0

Query: 17  FANCSTVRYCLRRNDGADEQVLQVRVGYVSRLISRSP-SGGLPYDIIGESPWSPVADGEF 76
           F + S  RY +RR D +++Q L +++G V + +SR P  G   ++I+ ES   P+    F
Sbjct: 5   FQSDSISRYTVRRCDDSNDQSLLIQLGIVHQQLSRPPYHGPTNHNILHESNTVPLRITVF 64

Query: 77  PLPLRELEDPLLCEAYVVELLLSWFKLERATCNVMARKVTSLVGEILGEGNYDVKFQAVA 136
           PLPL+EL+DP +C  Y  + LL++  L+  TC+++A+ +TSL+GE+L  GN +VKF+ VA
Sbjct: 65  PLPLQELQDPSICHLY-TQRLLAYVDLDIETCDIVAKDITSLIGEVLAAGNLEVKFRIVA 124

Query: 137 DIEYIHMFWAEERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREIKREHSIKECSI 196
            I+ + M W EE  +    +        RR     ++M EK        +    + +C +
Sbjct: 125 LIDRVQMIWREETGKSRGGS--------RRAAIERLMMMEKF----DGFEEGRELGDCCV 184

Query: 197 CCDELL---NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           C +EL     +V++I CGHVYHKSCI+ W   ++SCPLCR+KI
Sbjct: 185 CYEELKGEEKEVSRIGCGHVYHKSCIINWVLRNDSCPLCRSKI 214

BLAST of Lag0011393 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-09
Identity = 19/47 (40.43%), Postives = 33/47 (70.21%), Query Frame = 0

Query: 188 HSIKECSICCDELL--NDVAKIPCGHVYHKSCILTWFHISNSCPLCR 233
           + + +C++C +E +   D  ++PC H+YHK CI+ W  ++NSCP+CR
Sbjct: 219 NDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCPICR 265

BLAST of Lag0011393 vs. TAIR 10
Match: AT5G54990.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.2e-09
Identity = 24/50 (48.00%), Postives = 34/50 (68.00%), Query Frame = 0

Query: 192 ECSICCDELLNDVA--KIPCGHVYHKSCILTWFHISNSCPLCRTKIKQQT 240
           +C IC  EL + V+  K+PC HV+H+ CI+TW   + SCP+CRTK   +T
Sbjct: 173 DCPICLTELSSGVSRMKLPCSHVFHRDCIMTWLKKNPSCPICRTKAHGKT 222

BLAST of Lag0011393 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.4e-08
Identity = 20/48 (41.67%), Postives = 34/48 (70.83%), Query Frame = 0

Query: 190 IKECSICCDELL--NDVAKIPCGHVYHKSCILTWFHISNSCPLCRTKI 236
           + +C++C DE    +DV ++PC HV+H+ C+L W  + NSCP+CR ++
Sbjct: 213 MNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of Lag0011393 vs. TAIR 10
Match: AT4G12210.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-08
Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 0

Query: 176 KVVLVAREIKREH------SIKECSICCDELL-----NDVAKIPCGHVYHKSCILTWFHI 235
           ++VL+ R IK E         + CSIC + L+     +D+ ++ C HV+H  C+L WF  
Sbjct: 131 RIVLLGR-IKAEELKSLKMETESCSICLENLVSGPKPSDLTRMTCSHVFHNPCLLEWFMR 190

BLAST of Lag0011393 vs. TAIR 10
Match: AT1G18760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 56.6 bits (135), Expect = 3.1e-08
Identity = 36/95 (37.89%), Postives = 50/95 (52.63%), Query Frame = 0

Query: 146 EERRRRSSENVNVNVNVGRRGHSHGVIMEEKVVLVAREI--KREHSIKECSICCDELLND 205
           EE  R S E  N   N+  R  +  V+       +AR+I  K   S + C+IC +E  ND
Sbjct: 134 EEAVRASLEETN---NISLRPANKLVVNS-----LARKIYKKTTSSTERCTICLEE-FND 193

Query: 206 VAKI---PCGHVYHKSCILTWFHISNSCPLCRTKI 236
             K+   PCGH +   C+LTWF  ++ CPLCR K+
Sbjct: 194 GTKVMTLPCGHEFDDECVLTWFETNHDCPLCRFKL 219

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022947841.11.4e-5555.41NEP1-interacting protein-like 1 [Cucurbita moschata][more]
KAG6605222.11.4e-5555.41E3 ubiquitin-protein ligase AMFR, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023006825.12.1e-5454.11NEP1-interacting protein-like 1 [Cucurbita maxima][more]
KAG6605180.13.0e-5354.19E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022947853.12.6e-5254.19E3 ubiquitin-protein ligase MBR2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9VHI72.0e-0739.34E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1... [more]
Q8LPN72.0e-0741.67E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q95SP22.6e-0737.74E3 ubiquitin-protein ligase HRD1 OS=Drosophila melanogaster OX=7227 GN=sip3 PE=1... [more]
Q75DC24.4e-0748.94Alcohol-sensitive RING finger protein 1 OS=Ashbya gossypii (strain ATCC 10895 / ... [more]
Q9LZJ64.4e-0739.39RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G7L17.0e-5655.41NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE... [more]
A0A6J1L1861.0e-5454.11NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4... [more]
A0A6J1G7R81.2e-5254.19E3 ubiquitin-protein ligase MBR2-like OS=Cucurbita moschata OX=3662 GN=LOC111451... [more]
A0A6J1L1913.6e-5252.32E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3BYE91.1e-3237.67RING finger protein 165-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT1G60360.11.7e-0940.43RING/U-box superfamily protein [more]
AT5G54990.12.2e-0948.00RING/U-box superfamily protein [more]
AT3G19950.11.4e-0841.67RING/U-box superfamily protein [more]
AT4G12210.11.8e-0838.03RING/U-box superfamily protein [more]
AT1G18760.13.1e-0837.89Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 193..231
e-value: 4.2E-6
score: 36.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 191..232
e-value: 4.5E-11
score: 42.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 193..232
score: 12.754832
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 153..238
e-value: 2.9E-16
score: 60.9
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 153..234
NoneNo IPR availablePANTHERPTHR15710:SF137ZINC FINGER, C3HC4 TYPE (RING FINGER) PROTEINcoord: 153..234
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 192..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011393.1Lag0011393.1mRNA