Lag0011286 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0011286
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr1: 20088517 .. 20089319 (-)
RNA-Seq Expression	Lag0011286
Synteny	Lag0011286

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGGGGTGGTGGTGGCTGTTGGCGATGAAGAAGGTGGCGCTGGCAACAACGGTGACTGGCGAGAAAAGGAGAGAGAGAGGGATTTGGTGGTGGAAAGAAAGCCATAAAAGAGATAGGGAGAGAGACTTTCCATGGAGGGGGGAGGGGTAGATTTTTTTTTATAATTTTTATAATTTTATTATTTGGTTTAATTTTAAAATATTATTTTATTTTATAAATGTACCACGTCAACATCCATTAGCAACACACCCGAACAGTGGGGACAAAAAAGGGTATTTTACTTTTTAAAATTCAAATTTCCACAAAATTTTCATTTTCCACTTGTCTTTTGCGTGCAATAGAGCGGTGGCTCCTTCCACCACATGCTTATAAAATCAAACAAATTTTCATTAGACCTCATCTTATCCCGAACTCCAAACATTGGTTCACATAAATGGAATTTTCTCAACTCTCTTCACCAGCCGCTGTCACCGGAATCTTCGCCCTTCTCTTCTGTCTCTATGCTTTCTTCAACATATCTGCAAGAGTCGGAGGTCATCGGAAGATGCTCCCACCTGAACCCGCCGGCGGTTGGCCGTTGATCGGCCACCTCCATCTGCTAAGTGCAAGAGAGCCAGCTCACATAACCTTTGCAAAGATGGCTGACACCTACGGACCAATCTTTACATTGAGATTGGGTATGAAGAGAGCGTTGATTGTGAGCAATTGGAAAATAGCGAGAGAGTGCTTTACTACCAACGACAGAATCTTCACTTCTCGTCCAAAGCTTGCGGCCTCAAAGCTTCTAGGTTATGACTAA

mRNA sequence

ATGGGAGGGGTGGTGGTGGCTGTTGGCGATGAAGAAGGTGGCGCTGGCAACAACGCCGCTGTCACCGGAATCTTCGCCCTTCTCTTCTGTCTCTATGCTTTCTTCAACATATCTGCAAGAGTCGGAGGTCATCGGAAGATGCTCCCACCTGAACCCGCCGGCGGTTGGCCGTTGATCGGCCACCTCCATCTGCTAAGTGCAAGAGAGCCAGCTCACATAACCTTTGCAAAGATGGCTGACACCTACGGACCAATCTTTACATTGAGATTGGGTATGAAGAGAGCGTTGATTGTGAGCAATTGGAAAATAGCGAGAGAGTGCTTTACTACCAACGACAGAATCTTCACTTCTCGTCCAAAGCTTGCGGCCTCAAAGCTTCTAGGTTATGACTAA

Coding sequence (CDS)

ATGGGAGGGGTGGTGGTGGCTGTTGGCGATGAAGAAGGTGGCGCTGGCAACAACGCCGCTGTCACCGGAATCTTCGCCCTTCTCTTCTGTCTCTATGCTTTCTTCAACATATCTGCAAGAGTCGGAGGTCATCGGAAGATGCTCCCACCTGAACCCGCCGGCGGTTGGCCGTTGATCGGCCACCTCCATCTGCTAAGTGCAAGAGAGCCAGCTCACATAACCTTTGCAAAGATGGCTGACACCTACGGACCAATCTTTACATTGAGATTGGGTATGAAGAGAGCGTTGATTGTGAGCAATTGGAAAATAGCGAGAGAGTGCTTTACTACCAACGACAGAATCTTCACTTCTCGTCCAAAGCTTGCGGCCTCAAAGCTTCTAGGTTATGACTAA

Protein sequence

MGGVVVAVGDEEGGAGNNAAVTGIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMADTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD

Homology

[Close]

BLAST of Lag0011286 vs. NCBI nr
Match: XP_008464766.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032728.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98760.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 185.7 bits (470), Expect = 2.6e-43
Identity = 87/111 (78.38%), Postives = 97/111 (87.39%), Query Frame = 0


Query: 21  VTGIFALLFCLYAFFNISAR-VGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMA 80
           VTGIF+LL  LY  FNIS R VG HRK LPPEPAG WP+IGHLHLLSA EPAHIT AK+A
Sbjct: 13  VTGIFSLLLFLYTLFNISTRVVGAHRKKLPPEPAGHWPVIGHLHLLSATEPAHITLAKLA 72

Query: 81  DTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           DTYGPIFTL+LGM +AL+VS+W+IA+ECFTTNDRIF SRPKL ASKLLGY+
Sbjct: 73  DTYGPIFTLKLGMSKALVVSSWEIAKECFTTNDRIFASRPKLVASKLLGYN 123

[Close]

BLAST of Lag0011286 vs. NCBI nr
Match: XP_038896981.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 183.3 bits (464), Expect = 1.3e-42
Identity = 85/111 (76.58%), Postives = 96/111 (86.49%), Query Frame = 0


Query: 21  VTGIFALLFCLYAFFNISARV-GGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMA 80
           + GIF+++  LYA F IS RV G HR  LPPEP GGWPLIGHLHLL+A EPAHITFAK+A
Sbjct: 13  IAGIFSVVLFLYALFIISTRVIGAHRNKLPPEPVGGWPLIGHLHLLNAAEPAHITFAKLA 72

Query: 81  DTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           DTYGPIFTL+LGMK+ALIVS+W+IA ECFTTND+IF SRPKL ASKLLGYD
Sbjct: 73  DTYGPIFTLKLGMKKALIVSSWEIANECFTTNDKIFASRPKLVASKLLGYD 123

[Close]

BLAST of Lag0011286 vs. NCBI nr
Match: XP_022940489.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 182.6 bits (462), Expect = 2.2e-42
Identity = 86/114 (75.44%), Postives = 95/114 (83.33%), Query Frame = 0


Query: 17  NNAAVTGIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFA 76
           ++AAV GIFALL  LY  FNIS R+  + K LPPEPAG WP+IGHLHLLS  EPAHIT A
Sbjct: 8   SSAAVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITLA 67

Query: 77  KMADTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           KMAD YGPIFTLRLGM +ALIVS+W+IARECFTT DRIF SRPKL ASKLLGY+
Sbjct: 68  KMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYN 121

[Close]

BLAST of Lag0011286 vs. NCBI nr
Match: XP_023523452.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 181.8 bits (460), Expect = 3.8e-42
Identity = 86/114 (75.44%), Postives = 95/114 (83.33%), Query Frame = 0


Query: 17  NNAAVTGIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFA 76
           ++AAV GIFALL  LYA FNI  R+  + K LPPEPAG WP+IGHLHLLS  EPAHIT A
Sbjct: 8   SSAAVAGIFALLIFLYALFNIYKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITLA 67

Query: 77  KMADTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           KMAD YGPIFTLRLGM +ALIVS+W+IARECFTT DRIF SRPKL ASKLLGY+
Sbjct: 68  KMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYN 121

[Close]

BLAST of Lag0011286 vs. NCBI nr
Match: XP_022982223.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 180.3 bits (456), Expect = 1.1e-41
Identity = 84/114 (73.68%), Postives = 94/114 (82.46%), Query Frame = 0


Query: 17  NNAAVTGIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFA 76
           ++AAV GIF LL  LYA FNIS ++  + K LPPEPAG WP+IGHLHLLS  EPAHIT A
Sbjct: 11  SSAAVAGIFTLLIFLYALFNISTKLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITLA 70

Query: 77  KMADTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           KMAD YGPIFTL+LGM R LIVS+W+IARECFTT DRIF SRPKL ASKLLGY+
Sbjct: 71  KMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYN 124

[Close]

BLAST of Lag0011286 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.1e-23
Identity = 50/108 (46.30%), Postives = 72/108 (66.67%), Query Frame = 0


Query: 23  GIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMADTY 82
           G F LL C   FF    +   ++   PP+PAG WP+IGHLHLL+  +  H   +  AD  
Sbjct: 13  GFFVLLPC---FFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKN 72

Query: 83  GPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           GP+F ++LG+ +AL+V+N +IA+ECFTTNDR F +RP   A+K++GY+
Sbjct: 73  GPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYN 117

[Close]

BLAST of Lag0011286 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 1.7e-21
Identity = 49/111 (44.14%), Postives = 70/111 (63.06%), Query Frame = 0


Query: 20  AVTGIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMA 79
           A   + +LLF L+ F  +S     H K  PP  +G WPL+GHL L+   +  H T   + 
Sbjct: 12  AFISLISLLFFLFRFSKVS-----HTKE-PPIISGSWPLLGHLPLMRNTQTPHKTLGALV 71

Query: 80  DTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           D YGPIFT++LG   AL++SNW++A+ECFT ND + +SRPK  A +L+ Y+
Sbjct: 72  DKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYN 116

[Close]

BLAST of Lag0011286 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-21
Identity = 47/108 (43.52%), Postives = 68/108 (62.96%), Query Frame = 0


Query: 23  GIFALLFCLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMADTY 82
           G+ +L+  LY F    +   G     PP  AG WP+IGHL LL   +  H T   +AD Y
Sbjct: 13  GVVSLILLLYLFLRGGSWKSGEEG--PPTVAGAWPIIGHLPLLLGSKTPHKTLGDLADKY 72

Query: 83  GPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
           GPIF++++G K A++VSNW++A+EC+TTND   +S P L ++ LL Y+
Sbjct: 73  GPIFSIKIGAKNAVVVSNWEMAKECYTTNDIAVSSLPDLISANLLCYN 118

[Close]

BLAST of Lag0011286 vs. ExPASy Swiss-Prot
Match: L7X3S1 (Methyltetrahydroprotoberberine 14-monooxygenase OS=Papaver somniferum OX=3469 GN=CYP82N4 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-21
Identity = 43/85 (50.59%), Postives = 59/85 (69.41%), Query Frame = 0


Query: 46  KMLPPEPAGGWPLIGHLHLLSAREPAHITFAKMADTYGPIFTLRLGMKRALIVSNWKIAR 105
           K L PE +GG P++GHLHL +  E  H     MADTYGP+F +R G  + L+VS+W+I +
Sbjct: 42  KKLAPEASGGRPIMGHLHLFNDGELTHRKLGAMADTYGPVFNIRFGSHKTLVVSDWEIVK 101

Query: 106 ECFTTNDRIFTSRPKLAASKLLGYD 131
           ECFTTND++F++RP     KL+ YD
Sbjct: 102 ECFTTNDKLFSNRPGTLGIKLMFYD 126

[Close]

BLAST of Lag0011286 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.8e-21
Identity = 48/115 (41.74%), Postives = 73/115 (63.48%), Query Frame = 0


Query: 17  NNAAVTGIFALLF-CLYAFFNISARVGGHRKMLPPEPAGGWPLIGHLHLLSAREPAHITF 76
           N  A+  I +L+F CL+ +   S      R    P  +G WP++GHL LL+  +  H   
Sbjct: 11  NTIAIASILSLIFLCLFLYRKNS------RGKDAPVVSGAWPILGHLSLLNGSQTPHKVL 70

Query: 77  AKMADTYGPIFTLRLGMKRALIVSNWKIARECFTTNDRIFTSRPKLAASKLLGYD 131
             +AD YGP+FT++LGMK AL++SNW++++E FTTND   +SRPKL A +++ Y+
Sbjct: 71  GALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYN 119

The following BLAST results are available for this feature:

BLAST of Lag0011286 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008464766.1	2.6e-43	78.38	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032728.1 cytochrome...	[more]
XP_038896981.1	1.3e-42	76.58	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_022940489.1	2.2e-42	75.44	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_023523452.1	3.8e-42	75.44	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982223.1	1.1e-41	73.68	cytochrome P450 CYP82D47-like [Cucurbita maxima]	[more]

BLAST of Lag0011286 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0N7F297	3.1e-23	46.30	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
Q43068	1.7e-21	44.14	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
O81972	2.2e-21	43.52	Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1	[more]
L7X3S1	2.2e-21	50.59	Methyltetrahydroprotoberberine 14-monooxygenase OS=Papaver somniferum OX=3469 GN...	[more]
O49858	3.8e-21	41.74	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 79..98 score: 34.05 coord: 103..124 score: 22.97
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 50..122 e-value: 3.1E-9 score: 36.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 18..130 e-value: 2.4E-18 score: 68.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 44..129
None	No IPR available	PANTHER	PTHR24299	CYTOCHROME P450 FAMILY 1	coord: 28..130
None	No IPR available	PANTHER	PTHR24299:SF32	CYTOCHROME P450 82A4-LIKE	coord: 28..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0011286.1	Lag0011286.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen