Lag0011257 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0011257
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase
Location	chr1: 19157679 .. 19158581 (-)
RNA-Seq Expression	Lag0011257
Synteny	Lag0011257

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTTAGAGTAAGGGTTATTCCTCTGATTACCTACAAAAAACACAGAAGCTGGATTGTTGGGGCAAAACTCGTAGTTGTGATTTTCACCACAATAGACACATGCTTCCTCTACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGTTGAAGAAGTTAGGAGCCACAGTGTTTGAGGGATCCACAGATCTAGCTGACGCCGATGTCTGGTTAAATATGTTGGAGAAATGCTTCGACGTGATGAGTTGTCCTCAAGAGCGAAAAGTCTAATTAGCCACATTCCTTTTGCAGAAGGAGGCAGAGGGATGGTGGAAATCCATTATAGCCAGGCGCAGTGATGCACGTACGCTAGATTGGCAGACATTCAGAGGCATATTTGAGGAAAAGTACTATCCCGCCACATATTGTGAGGCAAAAAGAGATGAGTTTCTTGAGCTGAAACAAGGGTCACTTTCAGTGGTCGAGTACGAGAGGAAGTATACCGAGCTTTCACGGAATGCTGAAGTGATTGTGGCATCTGAGAGTGACAGGTGTCACAGGTTTGAGAGAGGGCTACGTTTTGAGATACGTACCCCAGTTACCGCAATTGCCAAGTGGACGAATTTTTCCCAGCTAGTAGAGACTGCCTTACGTGTGGAGCAGAGTATTACAGAGGAAAATTCGGTTGCAGAGCCTAGTCGTGGAGCTCCAACAGCTAGTGGTTTTCGAGGTGGTGAGCAGCGGAGGTTCACACATGGAGTGAATGTTTCAGGTCGTCAAGACTTCAAGCGTCGATCTGGTGGCCAGTTATCGAGGCAGATGAGTTCAGGTAGTGCCTATCAAAGGTAG

mRNA sequence

ATGAAAGTTAGAAAGGAGGCAGAGGGATGGTGGAAATCCATTATAGCCAGGCGCAGTGATGCACGTACGCTAGATTGGCAGACATTCAGAGGCATATTTGAGGAAAAGTACTATCCCGCCACATATTGTGAGGCAAAAAGAGATGAGTTTCTTGAGCTGAAACAAGGGTCACTTTCAGTGGTCGAGTACGAGAGGAAGTATACCGAGCTTTCACGGAATGCTGAAGTGATTGTGGCATCTGAGAGTGACAGGTGTCACAGGTTTGAGAGAGGGCTACGTTTTGAGATACGTACCCCAGTTACCGCAATTGCCAAGTGGACGAATTTTTCCCAGCTAGTAGAGACTGCCTTACGTGTGGAGCAGAGTATTACAGAGGAAAATTCGGTTGCAGAGCCTAGTCGTGGAGCTCCAACAGCTAGTGGTTTTCGAGGTGGTGAGCAGCGGAGGTTCACACATGGAGTGAATGTTTCAGGTCGTCAAGACTTCAAGCGTCGATCTGGTGGCCAGTTATCGAGGCAGATGAGTTCAGGTAGTGCCTATCAAAGGTAG

Coding sequence (CDS)

ATGAAAGTTAGAAAGGAGGCAGAGGGATGGTGGAAATCCATTATAGCCAGGCGCAGTGATGCACGTACGCTAGATTGGCAGACATTCAGAGGCATATTTGAGGAAAAGTACTATCCCGCCACATATTGTGAGGCAAAAAGAGATGAGTTTCTTGAGCTGAAACAAGGGTCACTTTCAGTGGTCGAGTACGAGAGGAAGTATACCGAGCTTTCACGGAATGCTGAAGTGATTGTGGCATCTGAGAGTGACAGGTGTCACAGGTTTGAGAGAGGGCTACGTTTTGAGATACGTACCCCAGTTACCGCAATTGCCAAGTGGACGAATTTTTCCCAGCTAGTAGAGACTGCCTTACGTGTGGAGCAGAGTATTACAGAGGAAAATTCGGTTGCAGAGCCTAGTCGTGGAGCTCCAACAGCTAGTGGTTTTCGAGGTGGTGAGCAGCGGAGGTTCACACATGGAGTGAATGTTTCAGGTCGTCAAGACTTCAAGCGTCGATCTGGTGGCCAGTTATCGAGGCAGATGAGTTCAGGTAGTGCCTATCAAAGGTAG

Protein sequence

MKVRKEAEGWWKSIIARRSDARTLDWQTFRGIFEEKYYPATYCEAKRDEFLELKQGSLSVVEYERKYTELSRNAEVIVASESDRCHRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQSITEENSVAEPSRGAPTASGFRGGEQRRFTHGVNVSGRQDFKRRSGGQLSRQMSSGSAYQR

Homology

[Close]

BLAST of Lag0011257 vs. NCBI nr
Match: KAA0037805.1 (uncharacterized protein E6C27_scaffold918G00190 [Cucumis melo var. makuwa])

HSP 1 Score: 301.2 bits (770), Expect = 6.0e-78
Identity = 151/180 (83.89%), Postives = 162/180 (90.00%), Query Frame = 0


Query: 3   VRKEAEGWWKSIIARRSDARTLDWQTFRGIFEEKYYPATYCEAKRDEFLELKQGSLSVVE 62
           ++KEAEGWWKSI+ARRSDAR LDWQTFRGIFE+KYYP+TYCEAKRDEFL LKQGSLSV E
Sbjct: 115 LQKEAEGWWKSILARRSDARALDWQTFRGIFEDKYYPSTYCEAKRDEFLGLKQGSLSVAE 174

Query: 63  YERKYTELSRNAEVIVASESDRCHRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 122
           YERKYTELSR A+VI+ASESDRC RFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS
Sbjct: 175 YERKYTELSRYADVIIASESDRCRRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 234

Query: 123 ITEENSVAEPSRGAPTASGFRGGEQRRFTHGVNVSGRQDFKRRSGGQLSRQMSSGSAYQR 182
           ITEE SV E SRG  TASGFRG EQRRFT G+N+S RQDFK RSGGQ SR +S GS +QR
Sbjct: 235 ITEEKSVVELSRGTSTASGFRGREQRRFTPGINISSRQDFKNRSGGQASRNVSHGSVFQR 294

[Close]

BLAST of Lag0011257 vs. NCBI nr
Match: TYK03091.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 299.3 bits (765), Expect = 2.3e-77
Identity = 154/180 (85.56%), Postives = 161/180 (89.44%), Query Frame = 0


Query: 3   VRKEAEGWWKSIIARRSDARTLDWQTFRGIFEEKYYPATYCEAKRDEFLELKQGSLSVVE 62
           ++KEAEGWWKSIIARR+DARTLDWQTFRGIFEEKYYP TYCEAKRDEFLELKQ SLSV +
Sbjct: 327 LQKEAEGWWKSIIARRNDARTLDWQTFRGIFEEKYYPTTYCEAKRDEFLELKQWSLSVAK 386

Query: 63  YERKYTELSRNAEVIVASESDRCHRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 122
           YERKYTELSR AE+IVASESDRCHRFERGLRFEIRTPVTAIAKW NFSQLVETALRV+QS
Sbjct: 387 YERKYTELSRYAEMIVASESDRCHRFERGLRFEIRTPVTAIAKWMNFSQLVETALRVKQS 446

Query: 123 ITEENSVAEPSRGAPTASGFRGGEQRRFTHGVNVSGRQDFKRRSGGQLSRQMSSGSAYQR 182
           I EE S  E SRG  T SG RG EQRRFT GVNVSG QDFKRRSGG+  RQMSSGSAYQR
Sbjct: 447 IVEEKSAMELSRGVSTTSGIRGREQRRFTPGVNVSGCQDFKRRSGGKPLRQMSSGSAYQR 506

[Close]

BLAST of Lag0011257 vs. NCBI nr
Match: KAA0066849.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 299.3 bits (765), Expect = 2.3e-77
Identity = 150/180 (83.33%), Postives = 161/180 (89.44%), Query Frame = 0


Query: 3   VRKEAEGWWKSIIARRSDARTLDWQTFRGIFEEKYYPATYCEAKRDEFLELKQGSLSVVE 62
           ++KEAEGWWKSI+ARRSDAR LDWQTFRGIFE+KYYP+TYCEAKRDEFL LKQGSLSV E
Sbjct: 153 LQKEAEGWWKSILARRSDARALDWQTFRGIFEDKYYPSTYCEAKRDEFLGLKQGSLSVAE 212

Query: 63  YERKYTELSRNAEVIVASESDRCHRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 122
           YERKYTELSR A+VI+ASESDRC RFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS
Sbjct: 213 YERKYTELSRYADVIIASESDRCRRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 272

Query: 123 ITEENSVAEPSRGAPTASGFRGGEQRRFTHGVNVSGRQDFKRRSGGQLSRQMSSGSAYQR 182
           ITEE S  E SRG  TASGFRG EQRRFT G+N+S RQDFK RSGGQ SR +S GS +QR
Sbjct: 273 ITEEKSAVELSRGTSTASGFRGREQRRFTPGINISSRQDFKNRSGGQASRNVSYGSVFQR 332

[Close]

BLAST of Lag0011257 vs. NCBI nr
Match: KAA0035455.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >KAA0046782.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK01987.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK03719.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK25877.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 299.3 bits (765), Expect = 2.3e-77
Identity = 150/180 (83.33%), Postives = 161/180 (89.44%), Query Frame = 0


Query: 3   VRKEAEGWWKSIIARRSDARTLDWQTFRGIFEEKYYPATYCEAKRDEFLELKQGSLSVVE 62
           ++KEAEGWWKSI+ARRSDAR LDWQTFRGIFE+KYYP+TYCEAKRDEFL LKQGSLSV E
Sbjct: 153 LQKEAEGWWKSILARRSDARALDWQTFRGIFEDKYYPSTYCEAKRDEFLGLKQGSLSVAE 212

Query: 63  YERKYTELSRNAEVIVASESDRCHRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 122
           YERKYTELSR A+VI+ASESDRC RFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS
Sbjct: 213 YERKYTELSRYADVIIASESDRCRRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 272

Query: 123 ITEENSVAEPSRGAPTASGFRGGEQRRFTHGVNVSGRQDFKRRSGGQLSRQMSSGSAYQR 182
           ITEE S  E SRG  TASGFRG EQRRFT G+N+S RQDFK RSGGQ SR +S GS +QR
Sbjct: 273 ITEEKSAVELSRGTSTASGFRGREQRRFTPGINISSRQDFKNRSGGQASRNVSYGSVFQR 332

[Close]

BLAST of Lag0011257 vs. NCBI nr
Match: TYK06334.1 (putative polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 299.3 bits (765), Expect = 2.3e-77
Identity = 150/180 (83.33%), Postives = 161/180 (89.44%), Query Frame = 0


Query: 3   VRKEAEGWWKSIIARRSDARTLDWQTFRGIFEEKYYPATYCEAKRDEFLELKQGSLSVVE 62
           ++KEAEGWWKSI+ARRSDAR LDWQTFRGIFE+KYYP+TYCEAKRDEFL LKQGSLSV E
Sbjct: 115 LQKEAEGWWKSILARRSDARALDWQTFRGIFEDKYYPSTYCEAKRDEFLGLKQGSLSVAE 174

Query: 63  YERKYTELSRNAEVIVASESDRCHRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 122
           YERKYTELSR A+VI+ASESDRC RFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS
Sbjct: 175 YERKYTELSRYADVIIASESDRCRRFERGLRFEIRTPVTAIAKWTNFSQLVETALRVEQS 234

Query: 123 ITEENSVAEPSRGAPTASGFRGGEQRRFTHGVNVSGRQDFKRRSGGQLSRQMSSGSAYQR 182
           ITEE S  E SRG  TASGFRG EQRRFT G+N+S RQDFK RSGGQ SR +S GS +QR
Sbjct: 235 ITEEKSAVELSRGTSTASGFRGREQRRFTPGINISSRQDFKNRSGGQASRNVSYGSVFQR 294

The following BLAST results are available for this feature:

BLAST of Lag0011257 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0037805.1	6.0e-78	83.89	uncharacterized protein E6C27_scaffold918G00190 [Cucumis melo var. makuwa]	[more]
TYK03091.1	2.3e-77	85.56	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0066849.1	2.3e-77	83.33	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0035455.1	2.3e-77	83.33	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >KAA0046782.1...	[more]
TYK06334.1	2.3e-77	83.33	putative polyprotein [Cucumis melo var. makuwa]	[more]

BLAST of Lag0011257 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005162	Retrotransposon gag domain	PFAM	PF03732	Retrotrans_gag	coord: 3..93 e-value: 2.7E-12 score: 46.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 162..182
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..182
None	No IPR available	PANTHER	PTHR34482	DNA DAMAGE-INDUCIBLE PROTEIN 1-LIKE	coord: 3..165
None	No IPR available	PANTHER	PTHR34482:SF4	POLYMERASES SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATED	coord: 3..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0011257.1	Lag0011257.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0008270	zinc ion binding