Lag0010338 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0010338
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionIRK-interacting protein
Locationchr9: 46480309 .. 46482485 (+)
RNA-Seq ExpressionLag0010338
SyntenyLag0010338
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGCGACCCAAATGTTTCAGAACGCCGGCGATGTCAGCCGCCAAGAAATTCAGGCCGCCATTGCTAAAGCTGTGGAGCTCAGGGCTCTTCATGCCGCTCTTATGCAAGGAAGTAGCCCTTCTAACCTCCGATTCCCTTCGGCTTCTCCTGTTTCTCATGCTGCTTCTCAATTCTCTGCTCAAGATTACCCTGTTTTCACCCCTGTGAGTTCAACTTCCACACTTTTTTTTTTTTTTTTGGGGTGAATGATTGTTCTTTTTCCCTTATTCTTTTTTTTGAAGTCGCGTTGTTTAATTTTGGACTTTCTTGATTTGAGCTACGTCTTTGATTGTTTGATTATGATTTATGGATCTTATTTGCATCATTGGCATGTGCCTGGCATTTATGGTTAGCATTTAATGACTTCTTTTTTTCCCCCTTGAGTTGAGGACATTTTAGGATCGCCTTCTTTCTTTTTCACATTCCTATCCGTCATTTTCTCTTGGTTGGTTTAATTGATGATATTGAAAATTTGGGTATGTTTTGGTAGCTGTGTACGAAATGATTTAGACTAACAAATACATTTATGAGGGGTTAACTGTCAGTCAAAGGTAGAGACAATTTATTTGGTGAGTAATTAAAACATGGGTTTCTGTGAAGTTGGTTGAATTTGGAAGTCTCTTTACAGAGTTATGAAGATGAACCAACGAATGGGTACCACCAAGTTCCGATGAGAATTGGGACTTTTTCAGGAAGCTGGGATGAGTATGGATTAGGAGGAGGAGGAAATGAAGATGAAACAGTTTTATCTGATTACAAGAAGGAGATTTCAAAAGGGTTGGCTTCTGACTTTACCAATTTGGAACCCCATATTTGTCCAGCTGAAGATCATAAGACTCTCACTGGATCTGCAGTTCAAACATCACCTGTGAACGATTACTTCAAATCATCCAGGAGGAACAGTTTGGGGGACTCGAAATCGGCTTCTTCGTGCAATCGATGCAGGCCTGCAATTATTACCAAGGAGAGTGACAATACAGCAAAGAATAGCAGGATTTCAAACACCATAGTGCCATTGACAGATTCTCATTTGGGAACTCAATCGCAACCGAAAAGTCGGGGAGTGATTTCTTGGCTGTTTCCACGGTTAAAGAAGAAGAACAAGAATGAGAATTCCCCAAACAGGACGGAGTCAGAGGATGTCTCTCAGATATTTAAGGATTTGGGGATAGTGTCGATTGAAACCTTGAAGAAAGAATTGATTGAGGCGAACGAGACGAGAGACGCTGCATTGATGGAGGTTGGGGAGATGAAGTCGTCTTTAGGTGAGCTGAGGCAGAAACTGGAGGGCTTAGAGACTTACTGTGAGGAACTGAAGAGAGCTTTGAAGCAAGCAACACTTGCAAGAGATCTCCAAACTCCTACCAATTTGCCAAAGAAAACAACATTGAGCGCGGGGACGAACGGAGAGAACCGAATGCCAGTGAGTGAGGAAGTAATGGTGGAAGGCTTCTTGCAGATAGTATCAGAAGCAAGACTATCAGTAAAACAATTCTGCAAAACACTTGTAAGCCAGATTGAAGAAACTGATAGTACCCTGATGGAGAATTTGAACTCACTTCTTCAACCTTACAAGTTATCTCTAAACTCCAAGTATTCAAGAGCAGTATTGTACCATTTGGAAGCTATTATCAATCAGGCACTCTACCAAGATTTTGAGAACTGTGTGTTTGAAAAGAATGGCTCTCCCAAGCTGTTAGACCCTCTGCAAGATCGCCAAGCACAGTTCTCGTCGTTCGTGGCGCTGCGAAACCTCAGCTGGAACGAAGTGTTGAGGAAGGGAACAAAATACTACAGTGAAGAATTCAGCAAGTTTTGTGATCAAAAGATGAGCTGCATAATCACAACTCTGAATTGGACCAGACCATGGCCAGAGCAACTGCTTCAAGCATTCTTTGTAGCTGCCAAATGCATTTGGCTTCTTCACTTGCTCGCCTTCTCGTTCGATCCGCCGCTTAAAATCCTGAGAGTGGAAGAGAACAGAAGCTTCGATTCAAGTTACATGGAAGACATGTTTGCAGAGAGGCAGCGAAACGGTCCGAGTCGGGTTAAGATCATGGTGATGCCAGGGTTCTATGTACAAGATAGAATTTTAAGGTGTAAAGTTGTTTGCAGGTATAAGTCACCAGCTTAA

mRNA sequence

ATGCCTGCGACCCAAATGTTTCAGAACGCCGGCGATGTCAGCCGCCAAGAAATTCAGGCCGCCATTGCTAAAGCTGTGGAGCTCAGGGCTCTTCATGCCGCTCTTATGCAAGGAAGTAGCCCTTCTAACCTCCGATTCCCTTCGGCTTCTCCTGTTTCTCATGCTGCTTCTCAATTCTCTGCTCAAGATTACCCTGTTTTCACCCCTAGTTATGAAGATGAACCAACGAATGGGTACCACCAAGTTCCGATGAGAATTGGGACTTTTTCAGGAAGCTGGGATGAGTATGGATTAGGAGGAGGAGGAAATGAAGATGAAACAGTTTTATCTGATTACAAGAAGGAGATTTCAAAAGGGTTGGCTTCTGACTTTACCAATTTGGAACCCCATATTTGTCCAGCTGAAGATCATAAGACTCTCACTGGATCTGCAGTTCAAACATCACCTGTGAACGATTACTTCAAATCATCCAGGAGGAACAGTTTGGGGGACTCGAAATCGGCTTCTTCGTGCAATCGATGCAGGCCTGCAATTATTACCAAGGAGAGTGACAATACAGCAAAGAATAGCAGGATTTCAAACACCATAGTGCCATTGACAGATTCTCATTTGGGAACTCAATCGCAACCGAAAAGTCGGGGAGTGATTTCTTGGCTGTTTCCACGGTTAAAGAAGAAGAACAAGAATGAGAATTCCCCAAACAGGACGGAGTCAGAGGATGTCTCTCAGATATTTAAGGATTTGGGGATAGTGTCGATTGAAACCTTGAAGAAAGAATTGATTGAGGCGAACGAGACGAGAGACGCTGCATTGATGGAGGTTGGGGAGATGAAGTCGTCTTTAGGTGAGCTGAGGCAGAAACTGGAGGGCTTAGAGACTTACTGTGAGGAACTGAAGAGAGCTTTGAAGCAAGCAACACTTGCAAGAGATCTCCAAACTCCTACCAATTTGCCAAAGAAAACAACATTGAGCGCGGGGACGAACGGAGAGAACCGAATGCCAGTGAGTGAGGAAGTAATGGTGGAAGGCTTCTTGCAGATAGTATCAGAAGCAAGACTATCAGTAAAACAATTCTGCAAAACACTTGTAAGCCAGATTGAAGAAACTGATAGTACCCTGATGGAGAATTTGAACTCACTTCTTCAACCTTACAAGTTATCTCTAAACTCCAAGTATTCAAGAGCAGTATTGTACCATTTGGAAGCTATTATCAATCAGGCACTCTACCAAGATTTTGAGAACTGTGTGTTTGAAAAGAATGGCTCTCCCAAGCTGTTAGACCCTCTGCAAGATCGCCAAGCACAGTTCTCGTCGTTCGTGGCGCTGCGAAACCTCAGCTGGAACGAAGTGTTGAGGAAGGGAACAAAATACTACAGTGAAGAATTCAGCAAGTTTTGTGATCAAAAGATGAGCTGCATAATCACAACTCTGAATTGGACCAGACCATGGCCAGAGCAACTGCTTCAAGCATTCTTTGTAGCTGCCAAATGCATTTGGCTTCTTCACTTGCTCGCCTTCTCGTTCGATCCGCCGCTTAAAATCCTGAGAGTGGAAGAGAACAGAAGCTTCGATTCAAGTTACATGGAAGACATGTTTGCAGAGAGGCAGCGAAACGGTCCGAGTCGGGTTAAGATCATGGTGATGCCAGGGTTCTATGTACAAGATAGAATTTTAAGGTGTAAAGTTGTTTGCAGGTATAAGTCACCAGCTTAA

Coding sequence (CDS)

ATGCCTGCGACCCAAATGTTTCAGAACGCCGGCGATGTCAGCCGCCAAGAAATTCAGGCCGCCATTGCTAAAGCTGTGGAGCTCAGGGCTCTTCATGCCGCTCTTATGCAAGGAAGTAGCCCTTCTAACCTCCGATTCCCTTCGGCTTCTCCTGTTTCTCATGCTGCTTCTCAATTCTCTGCTCAAGATTACCCTGTTTTCACCCCTAGTTATGAAGATGAACCAACGAATGGGTACCACCAAGTTCCGATGAGAATTGGGACTTTTTCAGGAAGCTGGGATGAGTATGGATTAGGAGGAGGAGGAAATGAAGATGAAACAGTTTTATCTGATTACAAGAAGGAGATTTCAAAAGGGTTGGCTTCTGACTTTACCAATTTGGAACCCCATATTTGTCCAGCTGAAGATCATAAGACTCTCACTGGATCTGCAGTTCAAACATCACCTGTGAACGATTACTTCAAATCATCCAGGAGGAACAGTTTGGGGGACTCGAAATCGGCTTCTTCGTGCAATCGATGCAGGCCTGCAATTATTACCAAGGAGAGTGACAATACAGCAAAGAATAGCAGGATTTCAAACACCATAGTGCCATTGACAGATTCTCATTTGGGAACTCAATCGCAACCGAAAAGTCGGGGAGTGATTTCTTGGCTGTTTCCACGGTTAAAGAAGAAGAACAAGAATGAGAATTCCCCAAACAGGACGGAGTCAGAGGATGTCTCTCAGATATTTAAGGATTTGGGGATAGTGTCGATTGAAACCTTGAAGAAAGAATTGATTGAGGCGAACGAGACGAGAGACGCTGCATTGATGGAGGTTGGGGAGATGAAGTCGTCTTTAGGTGAGCTGAGGCAGAAACTGGAGGGCTTAGAGACTTACTGTGAGGAACTGAAGAGAGCTTTGAAGCAAGCAACACTTGCAAGAGATCTCCAAACTCCTACCAATTTGCCAAAGAAAACAACATTGAGCGCGGGGACGAACGGAGAGAACCGAATGCCAGTGAGTGAGGAAGTAATGGTGGAAGGCTTCTTGCAGATAGTATCAGAAGCAAGACTATCAGTAAAACAATTCTGCAAAACACTTGTAAGCCAGATTGAAGAAACTGATAGTACCCTGATGGAGAATTTGAACTCACTTCTTCAACCTTACAAGTTATCTCTAAACTCCAAGTATTCAAGAGCAGTATTGTACCATTTGGAAGCTATTATCAATCAGGCACTCTACCAAGATTTTGAGAACTGTGTGTTTGAAAAGAATGGCTCTCCCAAGCTGTTAGACCCTCTGCAAGATCGCCAAGCACAGTTCTCGTCGTTCGTGGCGCTGCGAAACCTCAGCTGGAACGAAGTGTTGAGGAAGGGAACAAAATACTACAGTGAAGAATTCAGCAAGTTTTGTGATCAAAAGATGAGCTGCATAATCACAACTCTGAATTGGACCAGACCATGGCCAGAGCAACTGCTTCAAGCATTCTTTGTAGCTGCCAAATGCATTTGGCTTCTTCACTTGCTCGCCTTCTCGTTCGATCCGCCGCTTAAAATCCTGAGAGTGGAAGAGAACAGAAGCTTCGATTCAAGTTACATGGAAGACATGTTTGCAGAGAGGCAGCGAAACGGTCCGAGTCGGGTTAAGATCATGGTGATGCCAGGGTTCTATGTACAAGATAGAATTTTAAGGTGTAAAGTTGTTTGCAGGTATAAGTCACCAGCTTAA

Protein sequence

MPATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQDYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLASDFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITKESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA
Homology
BLAST of Lag0010338 vs. NCBI nr
Match: XP_023534028.1 (IRK-interacting protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1058.1 bits (2735), Expect = 2.6e-305
Identity = 535/568 (94.19%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           +T MFQN GD+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQFSAQ
Sbjct: 6   STHMFQNGGDISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLAS 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS +WDEYGLGGGGNEDETVLSDY+KEISKGL S
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISKGLGS 125

Query: 123 DFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITKE 182
           DF N EPHICPAE+HK LTGSAVQTSPVNDYFKSSRRNSLGDSKS SSCNRCRPA ITKE
Sbjct: 126 DFANSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPATITKE 185

Query: 183 SDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 242
           SDN AKNSR+SNTIVPLTDSHL TQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS
Sbjct: 186 SDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 245

Query: 243 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRAL 302
           QIFKDLGIVSIETLKKELIEANETRDAALMEVGE+KSSLGELRQKLEGLE+YCEELKRAL
Sbjct: 246 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEELKRAL 305

Query: 303 KQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 362
           KQAT ARDLQT TNLPKK TL+AGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQFCKTL
Sbjct: 306 KQATNARDLQTVTNLPKKITLNAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 365

Query: 363 VSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNGS 422
           VSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVF+KNGS
Sbjct: 366 VSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGS 425

Query: 423 PKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 482
           PKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP
Sbjct: 426 PKLLDPRQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 485

Query: 483 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPSR 542
           WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFD SYMEDMFAERQRNGPSR
Sbjct: 486 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRNGPSR 545

Query: 543 VKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           VKIMVMPGFYVQDRILRCKVVCRYKSPA
Sbjct: 546 VKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Lag0010338 vs. NCBI nr
Match: XP_022958158.1 (IRK-interacting protein [Cucurbita moschata])

HSP 1 Score: 1057.4 bits (2733), Expect = 4.5e-305
Identity = 535/568 (94.19%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           +T MFQN GD+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQFSAQ
Sbjct: 6   STHMFQNGGDISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLAS 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS +WDEYGLGGGGNEDETVLSDY+KEISKGL S
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISKGLGS 125

Query: 123 DFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITKE 182
           DFTN EPHICPAE+HK LTGSAVQTSPVNDYFKSSRRNSLGDSKS SSCNRCRPA ITKE
Sbjct: 126 DFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPATITKE 185

Query: 183 SDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 242
           SDN AKNSR+SNTIVPLTDSHL TQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS
Sbjct: 186 SDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 245

Query: 243 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRAL 302
           QIFKDLGIVSIETLKKELIEANETRDAALMEVGE+KSSLGELRQKLEGLE+YCEELKRAL
Sbjct: 246 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEELKRAL 305

Query: 303 KQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 362
           KQAT ARDLQT T+LPKK  LSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQFCKTL
Sbjct: 306 KQATNARDLQTVTDLPKKIALSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 365

Query: 363 VSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNGS 422
           VSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVF+KNGS
Sbjct: 366 VSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGS 425

Query: 423 PKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 482
           PKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP
Sbjct: 426 PKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 485

Query: 483 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPSR 542
           WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFD SYMEDMFAERQRNGPSR
Sbjct: 486 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRNGPSR 545

Query: 543 VKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           VKIMVMPGFYVQDRILRCKVVCRYKSPA
Sbjct: 546 VKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Lag0010338 vs. NCBI nr
Match: KAG7035839.1 (IRK-interacting protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1056.6 bits (2731), Expect = 7.6e-305
Identity = 535/568 (94.19%), Postives = 550/568 (96.83%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           +T MFQN GD+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQFSAQ
Sbjct: 6   STHMFQNGGDISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLAS 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS +WDEYGLGGGGNEDETVLSDY+KEISKGL S
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISKGLGS 125

Query: 123 DFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITKE 182
           DF N EPHICPAE+HK LTGSAVQTSPVNDYFKSSRRNSLGDSKS SSCNRCRPA ITKE
Sbjct: 126 DFANSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPATITKE 185

Query: 183 SDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 242
           SDN AKNSR+SNTIVPLTDSHL TQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS
Sbjct: 186 SDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 245

Query: 243 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRAL 302
           QIFKDLGIVSIETLKKELIEANETRDAALMEVGE+KSSLGELRQKLEGLE+YCEELKRAL
Sbjct: 246 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEELKRAL 305

Query: 303 KQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 362
           KQAT ARDLQT TNLPKK TLSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQFCKTL
Sbjct: 306 KQATNARDLQTVTNLPKKITLSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 365

Query: 363 VSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNGS 422
           VSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVF+KNGS
Sbjct: 366 VSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGS 425

Query: 423 PKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 482
           PKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP
Sbjct: 426 PKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 485

Query: 483 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPSR 542
           WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFD SYMEDMFAERQRNGPSR
Sbjct: 486 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRNGPSR 545

Query: 543 VKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           VKIMVMPGFYVQ RILRCKVVCRYKSPA
Sbjct: 546 VKIMVMPGFYVQHRILRCKVVCRYKSPA 573

BLAST of Lag0010338 vs. NCBI nr
Match: XP_022995055.1 (IRK-interacting protein [Cucurbita maxima])

HSP 1 Score: 1053.9 bits (2724), Expect = 4.9e-304
Identity = 533/569 (93.67%), Postives = 550/569 (96.66%), Query Frame = 0

Query: 2   PATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSA 61
           P+  MFQN GD+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQFSA
Sbjct: 5   PSNHMFQNGGDISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQFSA 64

Query: 62  QDYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLA 121
           QDYPVFTPSYEDEPT GYHQVPMRIGTFS +W+EYGLGGGGNEDETVLSDY+KEISKGL 
Sbjct: 65  QDYPVFTPSYEDEPTTGYHQVPMRIGTFSETWEEYGLGGGGNEDETVLSDYRKEISKGLG 124

Query: 122 SDFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITK 181
           SDFTN EPHICPAE+HK LTGSAVQTSPVNDYFKSSRRNSLGDSKS SSCNRCRPA ITK
Sbjct: 125 SDFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPATITK 184

Query: 182 ESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDV 241
           ESDN AKNSR+SNTIVPLTDSHL TQSQ KSRGVISWLFPRLKKKNKNENSPNRTESEDV
Sbjct: 185 ESDNAAKNSRLSNTIVPLTDSHLATQSQSKSRGVISWLFPRLKKKNKNENSPNRTESEDV 244

Query: 242 SQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRA 301
           SQIFKDLGIVSIETLKKELIEANETRDAAL+EVGE+KSSLGELRQKLEGLE+YCEELKRA
Sbjct: 245 SQIFKDLGIVSIETLKKELIEANETRDAALLEVGEVKSSLGELRQKLEGLESYCEELKRA 304

Query: 302 LKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKT 361
           LKQAT ARDLQT TNLPKK TLSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQFCKT
Sbjct: 305 LKQATNARDLQTVTNLPKKITLSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQFCKT 364

Query: 362 LVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNG 421
           LVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVF+KNG
Sbjct: 365 LVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQKNG 424

Query: 422 SPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR 481
           SPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR
Sbjct: 425 SPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR 484

Query: 482 PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPS 541
           PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFD SYMEDMFAERQRNGPS
Sbjct: 485 PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRNGPS 544

Query: 542 RVKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           RVKIMVMPGFYVQDRILRCKVVCRYKSPA
Sbjct: 545 RVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Lag0010338 vs. NCBI nr
Match: XP_038901979.1 (IRK-interacting protein [Benincasa hispida])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.7e-302
Identity = 536/572 (93.71%), Postives = 550/572 (96.15%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           AT+MF+NAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ
Sbjct: 6   ATKMFRNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGL----GGGGNEDETVLSDYKKEISK 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS SWDEYGL    GGGGNEDET+LSDYKKEISK
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSESWDEYGLGGGGGGGGNEDETILSDYKKEISK 125

Query: 123 GLASDFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAI 182
           GLASDFTNLEPHICPAEDHK LTG   QTSP NDYFKSSRRNSLGDSKS SSCNRCRPAI
Sbjct: 126 GLASDFTNLEPHICPAEDHKPLTGYDFQTSPANDYFKSSRRNSLGDSKSVSSCNRCRPAI 185

Query: 183 ITKESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTES 242
           ITKESDNTAKNSR+SNTIVPLTDSHL TQSQPKSRGVIS LFPRLKKKNK ENSPNRTES
Sbjct: 186 ITKESDNTAKNSRLSNTIVPLTDSHLSTQSQPKSRGVISRLFPRLKKKNKYENSPNRTES 245

Query: 243 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEEL 302
           EDVSQIFKDLGIVSIETLKKELI+ANETRDAALMEV EMKSSLGELRQKLEGLE YCEEL
Sbjct: 246 EDVSQIFKDLGIVSIETLKKELIDANETRDAALMEVSEMKSSLGELRQKLEGLENYCEEL 305

Query: 303 KRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 362
           KRALKQAT ARDLQT TNLPK+   SAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF
Sbjct: 306 KRALKQATNARDLQTATNLPKRIPSSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 365

Query: 363 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFE 422
           CKTLVSQIEETD+TLMENLNS+LQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVF+
Sbjct: 366 CKTLVSQIEETDNTLMENLNSILQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQ 425

Query: 423 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 482
           KNGSPKLLDP QDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN
Sbjct: 426 KNGSPKLLDPHQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 485

Query: 483 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRN 542
           WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYM+D+FAERQ+N
Sbjct: 486 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMDDVFAERQKN 545

Query: 543 GPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           GPSRVKIMVMPGFYVQ++ILRCKVVCRYKSPA
Sbjct: 546 GPSRVKIMVMPGFYVQEKILRCKVVCRYKSPA 577

BLAST of Lag0010338 vs. ExPASy Swiss-Prot
Match: Q9LXU9 (IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 6.3e-161
Identity = 337/574 (58.71%), Postives = 414/574 (72.13%), Query Frame = 0

Query: 16  QEIQAAIAKAVELRALHAALMQ-GSSPS---NLRFPS----ASPVSHAASQFSAQDYPVF 75
           +E + AIA  V+LRAL A+LMQ  SSPS   +LR PS    ASP S      SA DYPVF
Sbjct: 12  REKEEAIANGVKLRALQASLMQMKSSPSSNYSLRNPSSSSAASPASRPLPNLSAHDYPVF 71

Query: 76  TPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSD-YKKEISKGLASDFTN 135
           TPSYEDEP + +H   +   T S +WDE G+  G  + +T LSD YK   S+      T 
Sbjct: 72  TPSYEDEPVSAFHHKNL---TLSETWDEDGV--GLVDGDTYLSDSYKTSTSRK-----TV 131

Query: 136 LEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRN-SLGDSKSASSCNRCRPAIITKESDN 195
           + PH      H   T S    SP   ++ + R N    D +S SSCN        K+   
Sbjct: 132 MMPH--QDSHHHVYTMSDALRSPPLHFYTTGRSNCGSVDFRSVSSCND-----YNKQKGF 191

Query: 196 TAKNSRISNTIVPLTDSHLG-TQSQPKSRG--VISWLFPRLKKKNKNE---NSPNRTE-S 255
             K+ + SN +VPLTDSH     SQP++RG  V+SWLFP+LKKK K+    NSP+ TE S
Sbjct: 192 DTKSLKSSNLVVPLTDSHSAVVSSQPRNRGGRVMSWLFPKLKKKQKSNSIFNSPSITEKS 251

Query: 256 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEEL 315
           E+VS++ KD G   +E LK+EL+EAN +RDAAL +V EMKSSLGEL +KL+ LE+YC+ L
Sbjct: 252 EEVSEVLKDSG-SGVEKLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNL 311

Query: 316 KRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 375
           K+AL++AT   ++ +  N   ++  S   N E  MPVSEEVMVEGFLQIVSEARLS+KQF
Sbjct: 312 KKALREAT---EVVSQENSGGRS--SGKKNSE--MPVSEEVMVEGFLQIVSEARLSIKQF 371

Query: 376 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFE 435
            KTLVS+I+E DSTL+ N+N+LLQP+ LS  SKYS+ + YHLEAII+Q++YQDFENCVF+
Sbjct: 372 LKTLVSEIDEEDSTLIGNINTLLQPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQ 431

Query: 436 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 495
           KNG PKLLDP QDRQA FSSF +LRNLSWNEVL+KGTKYYS+EFS+FCD+KMS IITTLN
Sbjct: 432 KNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLN 491

Query: 496 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRN 555
           WTRPW EQ+LQAFFVAAKC+WLLHLLAFSF+P L ILRVEENR F+SS+MEDM A+RQR+
Sbjct: 492 WTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRS 551

Query: 556 ----GPSRVKIMVMPGFYVQDRILRCKVVCRYKS 569
               GP+RVK+MVMPGFYV DR+LRCKV+CRYKS
Sbjct: 552 ALSRGPARVKVMVMPGFYVLDRVLRCKVLCRYKS 560

BLAST of Lag0010338 vs. ExPASy Swiss-Prot
Match: F4KGE8 (Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.4e-06
Identity = 74/308 (24.03%), Postives = 127/308 (41.23%), Query Frame = 0

Query: 274 VGEMKSSLGELRQKLEGLETYCEELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRM 333
           V   ++ + EL+++++  +T  E LK  +K A++A       N  KK  L +        
Sbjct: 239 VAPYEAVVKELKKEVKVKDTEIENLKEKVKVASMANG-----NGGKKHRLLSSRKVNCTT 298

Query: 334 PVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSL-----------LQ 393
            ++   + E F   + + + + K F   L+S +      +   + S+             
Sbjct: 299 QIAVSPVPELFEMTMIQVKEASKSFTGILLSLMRAAHWDIAAAVRSIEAASASSDGMSAS 358

Query: 394 PYKLSLNSKY-SRAVLYHLEAIINQALYQDFENCVFEKNGS-PKLLDPLQDRQAQFSSFV 453
            +  S+ S   ++   + LE+ I + ++Q F++  F  +GS   L++P Q R+  F+ F 
Sbjct: 359 SFASSVQSSVPNQHAKFALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFK 418

Query: 454 ALRNLSWNEVLR----------KGTKYYS-----EEFSKFCDQKMSCIITTLNWTRPWPE 513
            ++ +   E+L              KY S      E S F D +   ++   N  R    
Sbjct: 419 DMKAMDPMELLGILPTCHFGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPR---S 478

Query: 514 QLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDM--FAERQRNGPSRV 552
           Q    F   AK +WLLHLLAFS DP            F S YME +  F++ +      V
Sbjct: 479 QFYGEFLGLAKAVWLLHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVV 538

BLAST of Lag0010338 vs. ExPASy TrEMBL
Match: A0A6J1H2D1 (IRK-interacting protein OS=Cucurbita moschata OX=3662 GN=LOC111459468 PE=4 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 2.2e-305
Identity = 535/568 (94.19%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           +T MFQN GD+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQFSAQ
Sbjct: 6   STHMFQNGGDISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLAS 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS +WDEYGLGGGGNEDETVLSDY+KEISKGL S
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSETWDEYGLGGGGNEDETVLSDYRKEISKGLGS 125

Query: 123 DFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITKE 182
           DFTN EPHICPAE+HK LTGSAVQTSPVNDYFKSSRRNSLGDSKS SSCNRCRPA ITKE
Sbjct: 126 DFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPATITKE 185

Query: 183 SDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 242
           SDN AKNSR+SNTIVPLTDSHL TQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS
Sbjct: 186 SDNAAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDVS 245

Query: 243 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRAL 302
           QIFKDLGIVSIETLKKELIEANETRDAALMEVGE+KSSLGELRQKLEGLE+YCEELKRAL
Sbjct: 246 QIFKDLGIVSIETLKKELIEANETRDAALMEVGEVKSSLGELRQKLEGLESYCEELKRAL 305

Query: 303 KQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 362
           KQAT ARDLQT T+LPKK  LSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQFCKTL
Sbjct: 306 KQATNARDLQTVTDLPKKIALSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQFCKTL 365

Query: 363 VSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNGS 422
           VSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVF+KNGS
Sbjct: 366 VSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQKNGS 425

Query: 423 PKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 482
           PKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP
Sbjct: 426 PKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTRP 485

Query: 483 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPSR 542
           WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFD SYMEDMFAERQRNGPSR
Sbjct: 486 WPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRNGPSR 545

Query: 543 VKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           VKIMVMPGFYVQDRILRCKVVCRYKSPA
Sbjct: 546 VKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Lag0010338 vs. ExPASy TrEMBL
Match: A0A6J1K320 (IRK-interacting protein OS=Cucurbita maxima OX=3661 GN=LOC111490720 PE=4 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.4e-304
Identity = 533/569 (93.67%), Postives = 550/569 (96.66%), Query Frame = 0

Query: 2   PATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSA 61
           P+  MFQN GD+SRQEIQAAIAKAVELRALHAALMQGSSPSN+RFPSASPVS AASQFSA
Sbjct: 5   PSNHMFQNGGDISRQEIQAAIAKAVELRALHAALMQGSSPSNVRFPSASPVSQAASQFSA 64

Query: 62  QDYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSDYKKEISKGLA 121
           QDYPVFTPSYEDEPT GYHQVPMRIGTFS +W+EYGLGGGGNEDETVLSDY+KEISKGL 
Sbjct: 65  QDYPVFTPSYEDEPTTGYHQVPMRIGTFSETWEEYGLGGGGNEDETVLSDYRKEISKGLG 124

Query: 122 SDFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAIITK 181
           SDFTN EPHICPAE+HK LTGSAVQTSPVNDYFKSSRRNSLGDSKS SSCNRCRPA ITK
Sbjct: 125 SDFTNSEPHICPAEEHKALTGSAVQTSPVNDYFKSSRRNSLGDSKSISSCNRCRPATITK 184

Query: 182 ESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESEDV 241
           ESDN AKNSR+SNTIVPLTDSHL TQSQ KSRGVISWLFPRLKKKNKNENSPNRTESEDV
Sbjct: 185 ESDNAAKNSRLSNTIVPLTDSHLATQSQSKSRGVISWLFPRLKKKNKNENSPNRTESEDV 244

Query: 242 SQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELKRA 301
           SQIFKDLGIVSIETLKKELIEANETRDAAL+EVGE+KSSLGELRQKLEGLE+YCEELKRA
Sbjct: 245 SQIFKDLGIVSIETLKKELIEANETRDAALLEVGEVKSSLGELRQKLEGLESYCEELKRA 304

Query: 302 LKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFCKT 361
           LKQAT ARDLQT TNLPKK TLSAGTNGEN+MPVSEEVMVEGFLQIVSEARLSVKQFCKT
Sbjct: 305 LKQATNARDLQTVTNLPKKITLSAGTNGENQMPVSEEVMVEGFLQIVSEARLSVKQFCKT 364

Query: 362 LVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEKNG 421
           LVSQIEETDSTLMENLN+LLQPYKL+LNSKYSRAVLYHLEAIINQALYQDFENCVF+KNG
Sbjct: 365 LVSQIEETDSTLMENLNALLQPYKLALNSKYSRAVLYHLEAIINQALYQDFENCVFQKNG 424

Query: 422 SPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR 481
           SPKLLDP QDR+A+FSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR
Sbjct: 425 SPKLLDPQQDREAKFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNWTR 484

Query: 482 PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNGPS 541
           PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFD SYMEDMFAERQRNGPS
Sbjct: 485 PWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDPSYMEDMFAERQRNGPS 544

Query: 542 RVKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           RVKIMVMPGFYVQDRILRCKVVCRYKSPA
Sbjct: 545 RVKIMVMPGFYVQDRILRCKVVCRYKSPA 573

BLAST of Lag0010338 vs. ExPASy TrEMBL
Match: A0A6J1D6H9 (IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111017373 PE=4 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.3e-302
Identity = 539/573 (94.07%), Postives = 551/573 (96.16%), Query Frame = 0

Query: 1   MPATQMFQNAG-DVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQF 60
           M ATQMFQN G +VSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQF
Sbjct: 1   MAATQMFQNGGNEVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQF 60

Query: 61  SAQDYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGL--GGGGNEDETVLSDYKKEIS 120
           SAQDYPVFTPSYEDEP+NGYHQVPMRIGTFS SWDEYGL  GGGGNEDETV SDY KE+S
Sbjct: 61  SAQDYPVFTPSYEDEPSNGYHQVPMRIGTFSESWDEYGLGGGGGGNEDETVFSDY-KEVS 120

Query: 121 KGLASDFTNLEPHICPAED-HKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRP 180
           KGLASDFTN+E HICPAED HK LTGSA+QTSP NDYFKSSRRNSLGDSKS SSCNRCRP
Sbjct: 121 KGLASDFTNVESHICPAEDLHKPLTGSALQTSPANDYFKSSRRNSLGDSKSTSSCNRCRP 180

Query: 181 AIITKESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRT 240
           A+ITKESDNTAKNSR+SNTIVPLTDSHL TQSQPKSRGVISWLFPRLKKKNK ENSPNRT
Sbjct: 181 AVITKESDNTAKNSRLSNTIVPLTDSHLATQSQPKSRGVISWLFPRLKKKNKTENSPNRT 240

Query: 241 ESEDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCE 300
           ESEDVSQIFKDLGIVS+ETL+KELIEANE RDAALMEV EMKSSLGELRQKLEGLETYCE
Sbjct: 241 ESEDVSQIFKDLGIVSMETLRKELIEANEMRDAALMEVSEMKSSLGELRQKLEGLETYCE 300

Query: 301 ELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVK 360
           ELKRALKQAT ARDLQTPTNLPK+TT + GTNGENRMPVSEEVMVEGFLQIVSEARLSVK
Sbjct: 301 ELKRALKQATHARDLQTPTNLPKRTTSNVGTNGENRMPVSEEVMVEGFLQIVSEARLSVK 360

Query: 361 QFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCV 420
           QFCKTLV QIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEA INQALYQDFENCV
Sbjct: 361 QFCKTLVGQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEATINQALYQDFENCV 420

Query: 421 FEKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITT 480
           F+KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITT
Sbjct: 421 FQKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITT 480

Query: 481 LNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQ 540
           LNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQ
Sbjct: 481 LNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQ 540

Query: 541 RNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSP 570
           RNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSP
Sbjct: 541 RNGPSRVKIMVMPGFYVQDRILRCKVVCRYKSP 572

BLAST of Lag0010338 vs. ExPASy TrEMBL
Match: A0A0A0KZA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G290210 PE=4 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.7e-292
Identity = 522/571 (91.42%), Postives = 538/571 (94.22%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           AT+MF NAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPS SPV    SQFSAQ
Sbjct: 6   ATKMFHNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSPSPV----SQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGL---GGGGNEDETVLSDYKKEISKG 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS SWDEYGL   GGGGN+DETVLSDYKKEISKG
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSESWDEYGLGGGGGGGNDDETVLSDYKKEISKG 125

Query: 123 LASDFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPAII 182
           L SDFTNLEPHICPAEDHK LTG + QTSP NDYFKSSRRNSLGDSKS SSCNRCRPAII
Sbjct: 126 LVSDFTNLEPHICPAEDHKPLTGFSFQTSPANDYFKSSRRNSLGDSKSVSSCNRCRPAII 185

Query: 183 TKESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTESE 242
           TKE+DNTAKNSR+SNTIVPLTDSHL TQSQPKSRGVIS LFPRLKKKNK ENSPNRTESE
Sbjct: 186 TKETDNTAKNSRLSNTIVPLTDSHLSTQSQPKSRGVISRLFPRLKKKNKYENSPNRTESE 245

Query: 243 DVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEELK 302
           DVSQIFKDLGIVSIETLKKELI ANETRDAALMEV EMK+SLGELRQKLEGLE YCEELK
Sbjct: 246 DVSQIFKDLGIVSIETLKKELINANETRDAALMEVSEMKTSLGELRQKLEGLENYCEELK 305

Query: 303 RALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQFC 362
           RALKQAT ARDLQT TNL K+ T ++G N ENRMPVSEEVMVEGFLQIVSEARLSVKQFC
Sbjct: 306 RALKQATNARDLQTATNLHKRITSTSGMNEENRMPVSEEVMVEGFLQIVSEARLSVKQFC 365

Query: 363 KTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFEK 422
           KTLVSQIEETD+TLMENLN +LQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVF+K
Sbjct: 366 KTLVSQIEETDNTLMENLNLILQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFQK 425

Query: 423 NGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNW 482
           NGSPKLLDP QDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNW
Sbjct: 426 NGSPKLLDPHQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLNW 485

Query: 483 TRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRNG 542
           TRPWPEQLLQAFFVA KCIWLLHLLAFSFDPPLKILRVEENRSFDSSYM+D+FAERQ+NG
Sbjct: 486 TRPWPEQLLQAFFVAGKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMDDVFAERQKNG 545

Query: 543 PSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           PSRVKIMVMPGFYVQD+ILRCKVVCRYKS A
Sbjct: 546 PSRVKIMVMPGFYVQDKILRCKVVCRYKSLA 572

BLAST of Lag0010338 vs. ExPASy TrEMBL
Match: A0A5D3B8I3 (IRK-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001300 PE=4 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.0e-291
Identity = 523/573 (91.27%), Postives = 539/573 (94.07%), Query Frame = 0

Query: 3   ATQMFQNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPVSHAASQFSAQ 62
           AT+MF NAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASP+    SQFSAQ
Sbjct: 6   ATKMFHNAGDVSRQEIQAAIAKAVELRALHAALMQGSSPSNLRFPSASPI----SQFSAQ 65

Query: 63  DYPVFTPSYEDEPTNGYHQVPMRIGTFSGSWDEYGL-----GGGGNEDETVLSDYKKEIS 122
           DYPVFTPSYEDEPTNGYHQVPMRIGTFS SWDEYGL     GGGGNEDETVLSDYKKEIS
Sbjct: 66  DYPVFTPSYEDEPTNGYHQVPMRIGTFSESWDEYGLGGGGGGGGGNEDETVLSDYKKEIS 125

Query: 123 KGLASDFTNLEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRNSLGDSKSASSCNRCRPA 182
           K    DFTNLEPHICPAEDHK LTG A Q+SPVNDYFKSSRRNSLGDSKS SSCNRCRPA
Sbjct: 126 K----DFTNLEPHICPAEDHKHLTGHAFQSSPVNDYFKSSRRNSLGDSKSVSSCNRCRPA 185

Query: 183 IITKESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRLKKKNKNENSPNRTE 242
           IITKE+DNTAKNSR+SNTIVPLTDSHL TQSQPKSRGVIS LFPRLKKKNK ENSPNRTE
Sbjct: 186 IITKETDNTAKNSRLSNTIVPLTDSHLSTQSQPKSRGVISRLFPRLKKKNKYENSPNRTE 245

Query: 243 SEDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEE 302
           SEDVSQIFKDLGIVSIETLKKELI ANETRDAALMEV EMK+SLGELRQKLEGLE YCEE
Sbjct: 246 SEDVSQIFKDLGIVSIETLKKELINANETRDAALMEVSEMKTSLGELRQKLEGLENYCEE 305

Query: 303 LKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQ 362
           LKRALKQAT ARDLQT TNL K+TT +AG N ENRMPVSEEVMVEGFLQIVSEARLSVKQ
Sbjct: 306 LKRALKQATNARDLQTATNLHKRTTSTAGMNEENRMPVSEEVMVEGFLQIVSEARLSVKQ 365

Query: 363 FCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVF 422
           FCKTLVSQIEETD+TLMENLNS+LQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVF
Sbjct: 366 FCKTLVSQIEETDNTLMENLNSILQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVF 425

Query: 423 EKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTL 482
           +KNGSPKLLDP QDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTL
Sbjct: 426 QKNGSPKLLDPHQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTL 485

Query: 483 NWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQR 542
           NWTRPWPEQLLQAFFVA KCIWLLHLLAFSFDPPLKILRVEENRSFDSSYM+D+FAERQ+
Sbjct: 486 NWTRPWPEQLLQAFFVAGKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMDDVFAERQK 545

Query: 543 NGPSRVKIMVMPGFYVQDRILRCKVVCRYKSPA 571
           NGPSRVKIMVMPGFYVQ++ILRCKVVCRYKS A
Sbjct: 546 NGPSRVKIMVMPGFYVQEKILRCKVVCRYKSLA 570

BLAST of Lag0010338 vs. TAIR 10
Match: AT5G12900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12330.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 568.9 bits (1465), Expect = 4.5e-162
Identity = 337/574 (58.71%), Postives = 414/574 (72.13%), Query Frame = 0

Query: 16  QEIQAAIAKAVELRALHAALMQ-GSSPS---NLRFPS----ASPVSHAASQFSAQDYPVF 75
           +E + AIA  V+LRAL A+LMQ  SSPS   +LR PS    ASP S      SA DYPVF
Sbjct: 12  REKEEAIANGVKLRALQASLMQMKSSPSSNYSLRNPSSSSAASPASRPLPNLSAHDYPVF 71

Query: 76  TPSYEDEPTNGYHQVPMRIGTFSGSWDEYGLGGGGNEDETVLSD-YKKEISKGLASDFTN 135
           TPSYEDEP + +H   +   T S +WDE G+  G  + +T LSD YK   S+      T 
Sbjct: 72  TPSYEDEPVSAFHHKNL---TLSETWDEDGV--GLVDGDTYLSDSYKTSTSRK-----TV 131

Query: 136 LEPHICPAEDHKTLTGSAVQTSPVNDYFKSSRRN-SLGDSKSASSCNRCRPAIITKESDN 195
           + PH      H   T S    SP   ++ + R N    D +S SSCN        K+   
Sbjct: 132 MMPH--QDSHHHVYTMSDALRSPPLHFYTTGRSNCGSVDFRSVSSCND-----YNKQKGF 191

Query: 196 TAKNSRISNTIVPLTDSHLG-TQSQPKSRG--VISWLFPRLKKKNKNE---NSPNRTE-S 255
             K+ + SN +VPLTDSH     SQP++RG  V+SWLFP+LKKK K+    NSP+ TE S
Sbjct: 192 DTKSLKSSNLVVPLTDSHSAVVSSQPRNRGGRVMSWLFPKLKKKQKSNSIFNSPSITEKS 251

Query: 256 EDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEGLETYCEEL 315
           E+VS++ KD G   +E LK+EL+EAN +RDAAL +V EMKSSLGEL +KL+ LE+YC+ L
Sbjct: 252 EEVSEVLKDSG-SGVEKLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNL 311

Query: 316 KRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEGFLQIVSEARLSVKQF 375
           K+AL++AT   ++ +  N   ++  S   N E  MPVSEEVMVEGFLQIVSEARLS+KQF
Sbjct: 312 KKALREAT---EVVSQENSGGRS--SGKKNSE--MPVSEEVMVEGFLQIVSEARLSIKQF 371

Query: 376 CKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIINQALYQDFENCVFE 435
            KTLVS+I+E DSTL+ N+N+LLQP+ LS  SKYS+ + YHLEAII+Q++YQDFENCVF+
Sbjct: 372 LKTLVSEIDEEDSTLIGNINTLLQPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQ 431

Query: 436 KNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKFCDQKMSCIITTLN 495
           KNG PKLLDP QDRQA FSSF +LRNLSWNEVL+KGTKYYS+EFS+FCD+KMS IITTLN
Sbjct: 432 KNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLN 491

Query: 496 WTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFAERQRN 555
           WTRPW EQ+LQAFFVAAKC+WLLHLLAFSF+P L ILRVEENR F+SS+MEDM A+RQR+
Sbjct: 492 WTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRS 551

Query: 556 ----GPSRVKIMVMPGFYVQDRILRCKVVCRYKS 569
               GP+RVK+MVMPGFYV DR+LRCKV+CRYKS
Sbjct: 552 ALSRGPARVKVMVMPGFYVLDRVLRCKVLCRYKS 560

BLAST of Lag0010338 vs. TAIR 10
Match: AT1G12330.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12900.1); Has 249 Blast hits to 249 proteins in 27 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 14; Plants - 217; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 250.0 bits (637), Expect = 4.6e-66
Identity = 148/410 (36.10%), Postives = 240/410 (58.54%), Query Frame = 0

Query: 164 DSKSASSCNRCRPAIITKESDNTAKNSRISNTIVPLTDSHLGTQSQPKSRGVISWLFPRL 223
           D   A SCN+CRP            + R   ++VPL +SH           +I  +F  L
Sbjct: 94  DDGGAVSCNKCRP----------HHSHRDKFSVVPL-ESHNNPSFISSPNLIIKSIFQSL 153

Query: 224 KKKNKNENS------PNRTESEDVSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEM 283
            +++   +S      P  + S       ++   +++  L  +LI+A + ++ A++E   +
Sbjct: 154 TRRSPKPSSATAALPPRSSSSSAADASREEQWRLAVAELSHKLIQATKKKEDAVIEASRL 213

Query: 284 KSSLGELRQKLEGLETYCEELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSE 343
           KSS+ EL +KL  LE YC  LK  L + +            KK ++    +G N      
Sbjct: 214 KSSMAELEKKLNKLEIYCHNLKSGLDECS-----------NKKQSVPIRKDGFN------ 273

Query: 344 EVMVEGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNS--KYSRA 403
           + +++ FL  VSE+R S++   ++L SQ+      + E L+ LLQP+ + +NS  K  ++
Sbjct: 274 DRIIQQFLVSVSESRSSIRALSRSLASQLRTVGGKVYERLSLLLQPFDVKINSFAKNPKS 333

Query: 404 VLYHLEAIINQALYQDFENCVFEKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGT 463
           ++++LEAI+++A ++DFE   F+KNGS ++L+P+   ++ ++SF  L  L+W+EVL +GT
Sbjct: 334 LIFYLEAILSRAFFEDFEAPGFQKNGSTRILNPIDRCESNYASFNVLMELTWDEVLSRGT 393

Query: 464 KYYSEEFSKFCDQKMSCIITTLNWTRPWPEQLLQAFFVAAKCIWLLHLLAFSFDPPLKIL 523
           K++SEEFS+FCD+KMS +++ L+W R WPE LLQAFF A+K +WL+HLLA S +P L+I 
Sbjct: 394 KHFSEEFSRFCDRKMSDVVSMLSWNRAWPEPLLQAFFGASKSVWLVHLLANSVNPGLQIF 453

Query: 524 RVEENRSFDSSYMEDMFAERQRNGPSRVKIMVMPGFYVQDRILRCKVVCR 566
           RVE++  FD  YME+   ER +   S V+ MV PGFYV   +++CKVVC+
Sbjct: 454 RVEKDDRFDPIYMEETGGERFK---SLVRAMVQPGFYVYGSVVKCKVVCK 472

BLAST of Lag0010338 vs. TAIR 10
Match: AT2G45260.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-13
Identity = 79/311 (25.40%), Postives = 132/311 (42.44%), Query Frame = 0

Query: 273 EVGEMKSSLGELRQKLEGLETYCEELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENR 332
           E+    S + ++ QK+E       +L++ LK     R + T          + G+NG+  
Sbjct: 131 EIQNKDSEITQMLQKIEEANQKRLKLEKNLK----LRGMST----------NEGSNGDGN 190

Query: 333 MPVSEEVMVEGFLQIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKY 392
           M    ++  E ++     A  +V  F K L++ ++     L    NS ++P  +     +
Sbjct: 191 MQF-PDLTTELYVSTYEAAAKAVHDFSKPLINMMKAAGWDLDSAANS-IEPDVVYAKRPH 250

Query: 393 SRAVLYHLEAIINQALYQDFENCVFEKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLR 452
            +   Y  E+ I Q ++  F+   F  N     +    D    F  F+AL+++   + L 
Sbjct: 251 KK---YAFESYICQRMFSGFQQKNFSVNSESAAVMANDDTDTFFRQFLALKDMDPLDAL- 310

Query: 453 KGTKYYSEEFSKFCDQKMSCII----------------TTLNWTRPWPEQLLQAFFVAAK 512
            GT   S  F  FC  K   ++                       P      QAF   AK
Sbjct: 311 -GTNPDS-NFGIFCRSKYLLLVHPKMEASFFGNLDQRDYVTGGGHP-RTAFYQAFLKLAK 370

Query: 513 CIWLLHLLAFSFDPPLKILRVEENRSFDSSYMEDMFA-----ERQRNGPSRVKIMVMPGF 563
            IW+LH LA+SFDP  KI +V++   F  SYME +       E++ N   RV +MVMPGF
Sbjct: 371 SIWILHRLAYSFDPAAKIFQVKKGSEFSDSYMESVVKNIVVDEKEEN--PRVGLMVMPGF 416

BLAST of Lag0010338 vs. TAIR 10
Match: AT3G14870.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 59.7 bits (143), Expect = 8.9e-09
Identity = 87/361 (24.10%), Postives = 138/361 (38.23%), Query Frame = 0

Query: 233 PNRTESED--VSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEG 292
           PN  +  D  V    K L  +    LKK+L + N  R   L E+ E++S L     ++ G
Sbjct: 115 PNGIQKADNLVVAELKTLSELKQSFLKKQL-DPNPDRTLVLAEIQELRSVL--KTYEIMG 174

Query: 293 LETYCEELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEG-----FL 352
            +  C+   +  +   L    Q      K        +G+   P+   + +       F+
Sbjct: 175 KKLECQLKLKDSEIIFLKEKFQESMTQNKLMEKRLNQSGQLCNPLDHNLHLSAVSSTHFV 234

Query: 353 QIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIIN 412
             +     S++ F K +V Q+ +  +  ++    L+QP  L     +     + LE  + 
Sbjct: 235 TYLHHTVKSIRGFVKLMVEQM-KLAAWDIDMAAELIQPDVLYYKQDHK---CFALEHYVC 294

Query: 413 QALYQDFENCVFEKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKF 472
           + + + F+   F    S K     +D+   F  F  LR++   E L    K      +KF
Sbjct: 295 KIMLEAFQLPYFSNESSKK--TSREDKAMFFERFTELRSMKPREYLASRPK---SRLAKF 354

Query: 473 CDQKMSCII--------------TTLNWTRPWPE-QLLQAFFVAAKCIWLLHLLAFSFDP 532
           C  K   +I                      +PE  L  AF   AK +WLLH LAFSFDP
Sbjct: 355 CRTKYLQLIHPKMEQAFFGHLHQRNQVTAGEFPETSLCTAFLEMAKRVWLLHCLAFSFDP 414

Query: 533 PLKILRVEENRSFDSSYM-----EDMFAERQRNGPSR----VKIMVMPGFYVQDRILRCK 563
              I +V     F   YM     E  F+  Q    S     V   V+PGF +    ++C+
Sbjct: 415 EASIFQVSRGCRFSEVYMKSVSEEAFFSPEQEESSSETEPGVAFTVVPGFRIGKTTIQCE 463

BLAST of Lag0010338 vs. TAIR 10
Match: AT3G14870.2 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 59.7 bits (143), Expect = 8.9e-09
Identity = 87/361 (24.10%), Postives = 138/361 (38.23%), Query Frame = 0

Query: 233 PNRTESED--VSQIFKDLGIVSIETLKKELIEANETRDAALMEVGEMKSSLGELRQKLEG 292
           PN  +  D  V    K L  +    LKK+L + N  R   L E+ E++S L     ++ G
Sbjct: 111 PNGIQKADNLVVAELKTLSELKQSFLKKQL-DPNPDRTLVLAEIQELRSVL--KTYEIMG 170

Query: 293 LETYCEELKRALKQATLARDLQTPTNLPKKTTLSAGTNGENRMPVSEEVMVEG-----FL 352
            +  C+   +  +   L    Q      K        +G+   P+   + +       F+
Sbjct: 171 KKLECQLKLKDSEIIFLKEKFQESMTQNKLMEKRLNQSGQLCNPLDHNLHLSAVSSTHFV 230

Query: 353 QIVSEARLSVKQFCKTLVSQIEETDSTLMENLNSLLQPYKLSLNSKYSRAVLYHLEAIIN 412
             +     S++ F K +V Q+ +  +  ++    L+QP  L     +     + LE  + 
Sbjct: 231 TYLHHTVKSIRGFVKLMVEQM-KLAAWDIDMAAELIQPDVLYYKQDHK---CFALEHYVC 290

Query: 413 QALYQDFENCVFEKNGSPKLLDPLQDRQAQFSSFVALRNLSWNEVLRKGTKYYSEEFSKF 472
           + + + F+   F    S K     +D+   F  F  LR++   E L    K      +KF
Sbjct: 291 KIMLEAFQLPYFSNESSKK--TSREDKAMFFERFTELRSMKPREYLASRPK---SRLAKF 350

Query: 473 CDQKMSCII--------------TTLNWTRPWPE-QLLQAFFVAAKCIWLLHLLAFSFDP 532
           C  K   +I                      +PE  L  AF   AK +WLLH LAFSFDP
Sbjct: 351 CRTKYLQLIHPKMEQAFFGHLHQRNQVTAGEFPETSLCTAFLEMAKRVWLLHCLAFSFDP 410

Query: 533 PLKILRVEENRSFDSSYM-----EDMFAERQRNGPSR----VKIMVMPGFYVQDRILRCK 563
              I +V     F   YM     E  F+  Q    S     V   V+PGF +    ++C+
Sbjct: 411 EASIFQVSRGCRFSEVYMKSVSEEAFFSPEQEESSSETEPGVAFTVVPGFRIGKTTIQCE 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023534028.12.6e-30594.19IRK-interacting protein [Cucurbita pepo subsp. pepo][more]
XP_022958158.14.5e-30594.19IRK-interacting protein [Cucurbita moschata][more]
KAG7035839.17.6e-30594.19IRK-interacting protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022995055.14.9e-30493.67IRK-interacting protein [Cucurbita maxima][more]
XP_038901979.12.7e-30293.71IRK-interacting protein [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LXU96.3e-16158.71IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1[more]
F4KGE82.4e-0624.03Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1H2D12.2e-30594.19IRK-interacting protein OS=Cucurbita moschata OX=3662 GN=LOC111459468 PE=4 SV=1[more]
A0A6J1K3202.4e-30493.67IRK-interacting protein OS=Cucurbita maxima OX=3661 GN=LOC111490720 PE=4 SV=1[more]
A0A6J1D6H91.3e-30294.07IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111017373 PE=4 SV=1[more]
A0A0A0KZA92.7e-29291.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G290210 PE=4 SV=1[more]
A0A5D3B8I34.0e-29191.27IRK-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT5G12900.14.5e-16258.71unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G12330.14.6e-6636.10unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplas... [more]
AT2G45260.11.2e-1325.40Plant protein of unknown function (DUF641) [more]
AT3G14870.18.9e-0924.10Plant protein of unknown function (DUF641) [more]
AT3G14870.28.9e-0924.10Plant protein of unknown function (DUF641) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 281..308
NoneNo IPR availableCOILSCoilCoilcoord: 253..273
NoneNo IPR availablePANTHERPTHR31029:SF3IRK-INTERACTING PROTEINcoord: 4..568
IPR042316IRK-interacting protein-likePANTHERPTHR31029CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 4..568

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0010338.1Lag0010338.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding