Lag0009588 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009588
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncoumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like
Locationchr9: 40661869 .. 40663272 (+)
RNA-Seq ExpressionLag0009588
SyntenyLag0009588
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGAAGAAATCATGAGTGACTCCAATTATACAGCGAAGGTATTAAAGCACAGCCAGATTTCTCCACCGGCGGGGTCAGTTCCCAGGACACACCTGCCCCTCACTTTCTTCGACGCCGTCTTCATTGCTTGCCCTCCCACGCATCGCTTTTTGTTCTACCAATTCCCTCACTCTACGCACCATTTCATGGAAGCCATTTTCCCCAATCTCGAGCGCTCCCTTTCTCTTTCTCTCCAACACTTCTTTCCTCTCGCCGGCAATTTCATCTTCCCTCCTCAACCCCACAACCCCCACATCCTCTTCAACACTCACGACTCTGTTCCGCTCACAGTGGCCGAGTCAACCGACGGCGATTTCGACCACCTCATCGCCGATTACCCTCGCGACGCCAAATACTTTTACCCCTTTGTTCCAGACGTCCCCGAGGCAAGTGTTTTGCACGACGGCACGCATGTGGTTCCGCTTCTGGCACTCCAAATCACCGTTTTCCCTAACCAAGGTCTCTGTATCTGTATCGCATTTCATCACGTGGTTGCCGATGGCATGGCAATTTTCCATTTTATGAAATCGTGGGCCTCCATATACAGAAATGGAGCTGCTGATTCGGGTTCGTTTGACGGACCGCTGCCGTACCTCGACAAGGCCTTCGTCAAGGACCCAAATGGCATTGAGTCGGTCTTTTTACAGGATTTGTCCGGTTGGGATTGGAACCCAGTCTTCGATAAGCCCATAGCGCCTGTTGGCAAGGTCAGGGCCACGTTCGTCCTCACCAAAACTCAAATCGAGAAATTGAAAATTTGGGTTTCAGCTCGAGAGTCGGAGCCTTTACGATTGTCGGCGTTCGTTGTTACATGCTCTCTCGTTTGGTGCTGTTTGATTCAAACATTAGAAAGCAAATCGAATTGCTTTCCAGATAAAGATGAGGAGTGCTATTTTTTATTCACAGCAGATTGTCGGAATCGGCTCGAGGATCCAATAATACCTTCGACATATTTTGGGAATTGCCTTGACATGTGTTTTGTGAAATCGAAGATGAAGGATTTGTTGGAACGTGGAAATATTGGTGCAGTGGCGAAGGCTATTGGAAACCGAGTGAAGGAATTTGAGAGAGAACCATTGAAAATGGCAGACAAGTGGTTGTCAGAGTTGATGCGATGTGAAGAATCGGGACGCTTTATAAATATTGCAGGGTCGCCTAAGTTGAGGGCTTATGATACAGATTTTGGGTGGGGAAGGCCGCTGAAAGTTGAAATGCTTCACATTGACACATCCAAGACAATTTCTCTACATGAATCAAGAGATGAGGAAGGTGGGATTGAAATTGGTTTGGGGCTCAGTAAGAGTCATATGGATCGCTTCACTACCGTATGGGAAGAGAATTTGAAGGTCCTCTGTAGTTAA

mRNA sequence

ATGTCTGAAGAAATCATGAGTGACTCCAATTATACAGCGAAGGTATTAAAGCACAGCCAGATTTCTCCACCGGCGGGGTCAGTTCCCAGGACACACCTGCCCCTCACTTTCTTCGACGCCGTCTTCATTGCTTGCCCTCCCACGCATCGCTTTTTGTTCTACCAATTCCCTCACTCTACGCACCATTTCATGGAAGCCATTTTCCCCAATCTCGAGCGCTCCCTTTCTCTTTCTCTCCAACACTTCTTTCCTCTCGCCGGCAATTTCATCTTCCCTCCTCAACCCCACAACCCCCACATCCTCTTCAACACTCACGACTCTGTTCCGCTCACAGTGGCCGAGTCAACCGACGGCGATTTCGACCACCTCATCGCCGATTACCCTCGCGACGCCAAATACTTTTACCCCTTTGTTCCAGACGTCCCCGAGGCAAGTGTTTTGCACGACGGCACGCATGTGGTTCCGCTTCTGGCACTCCAAATCACCGTTTTCCCTAACCAAGGTCTCTGTATCTGTATCGCATTTCATCACGTGGTTGCCGATGGCATGGCAATTTTCCATTTTATGAAATCGTGGGCCTCCATATACAGAAATGGAGCTGCTGATTCGGGTTCGTTTGACGGACCGCTGCCGTACCTCGACAAGGCCTTCGTCAAGGACCCAAATGGCATTGAGTCGGTCTTTTTACAGGATTTGTCCGGTTGGGATTGGAACCCAGTCTTCGATAAGCCCATAGCGCCTGTTGGCAAGGTCAGGGCCACGTTCGTCCTCACCAAAACTCAAATCGAGAAATTGAAAATTTGGGTTTCAGCTCGAGAGTCGGAGCCTTTACGATTGTCGGCGTTCGTTGTTACATGCTCTCTCGTTTGGTGCTGTTTGATTCAAACATTAGAAAGCAAATCGAATTGCTTTCCAGATAAAGATGAGGAGTGCTATTTTTTATTCACAGCAGATTGTCGGAATCGGCTCGAGGATCCAATAATACCTTCGACATATTTTGGGAATTGCCTTGACATGTGTTTTGTGAAATCGAAGATGAAGGATTTGTTGGAACGTGGAAATATTGGTGCAGTGGCGAAGGCTATTGGAAACCGAGTGAAGGAATTTGAGAGAGAACCATTGAAAATGGCAGACAAGTGGTTGTCAGAGTTGATGCGATGTGAAGAATCGGGACGCTTTATAAATATTGCAGGGTCGCCTAAGTTGAGGGCTTATGATACAGATTTTGGGTGGGGAAGGCCGCTGAAAGTTGAAATGCTTCACATTGACACATCCAAGACAATTTCTCTACATGAATCAAGAGATGAGGAAGGTGGGATTGAAATTGGTTTGGGGCTCAGTAAGAGTCATATGGATCGCTTCACTACCGTATGGGAAGAGAATTTGAAGGTCCTCTGTAGTTAA

Coding sequence (CDS)

ATGTCTGAAGAAATCATGAGTGACTCCAATTATACAGCGAAGGTATTAAAGCACAGCCAGATTTCTCCACCGGCGGGGTCAGTTCCCAGGACACACCTGCCCCTCACTTTCTTCGACGCCGTCTTCATTGCTTGCCCTCCCACGCATCGCTTTTTGTTCTACCAATTCCCTCACTCTACGCACCATTTCATGGAAGCCATTTTCCCCAATCTCGAGCGCTCCCTTTCTCTTTCTCTCCAACACTTCTTTCCTCTCGCCGGCAATTTCATCTTCCCTCCTCAACCCCACAACCCCCACATCCTCTTCAACACTCACGACTCTGTTCCGCTCACAGTGGCCGAGTCAACCGACGGCGATTTCGACCACCTCATCGCCGATTACCCTCGCGACGCCAAATACTTTTACCCCTTTGTTCCAGACGTCCCCGAGGCAAGTGTTTTGCACGACGGCACGCATGTGGTTCCGCTTCTGGCACTCCAAATCACCGTTTTCCCTAACCAAGGTCTCTGTATCTGTATCGCATTTCATCACGTGGTTGCCGATGGCATGGCAATTTTCCATTTTATGAAATCGTGGGCCTCCATATACAGAAATGGAGCTGCTGATTCGGGTTCGTTTGACGGACCGCTGCCGTACCTCGACAAGGCCTTCGTCAAGGACCCAAATGGCATTGAGTCGGTCTTTTTACAGGATTTGTCCGGTTGGGATTGGAACCCAGTCTTCGATAAGCCCATAGCGCCTGTTGGCAAGGTCAGGGCCACGTTCGTCCTCACCAAAACTCAAATCGAGAAATTGAAAATTTGGGTTTCAGCTCGAGAGTCGGAGCCTTTACGATTGTCGGCGTTCGTTGTTACATGCTCTCTCGTTTGGTGCTGTTTGATTCAAACATTAGAAAGCAAATCGAATTGCTTTCCAGATAAAGATGAGGAGTGCTATTTTTTATTCACAGCAGATTGTCGGAATCGGCTCGAGGATCCAATAATACCTTCGACATATTTTGGGAATTGCCTTGACATGTGTTTTGTGAAATCGAAGATGAAGGATTTGTTGGAACGTGGAAATATTGGTGCAGTGGCGAAGGCTATTGGAAACCGAGTGAAGGAATTTGAGAGAGAACCATTGAAAATGGCAGACAAGTGGTTGTCAGAGTTGATGCGATGTGAAGAATCGGGACGCTTTATAAATATTGCAGGGTCGCCTAAGTTGAGGGCTTATGATACAGATTTTGGGTGGGGAAGGCCGCTGAAAGTTGAAATGCTTCACATTGACACATCCAAGACAATTTCTCTACATGAATCAAGAGATGAGGAAGGTGGGATTGAAATTGGTTTGGGGCTCAGTAAGAGTCATATGGATCGCTTCACTACCGTATGGGAAGAGAATTTGAAGGTCCTCTGTAGTTAA

Protein sequence

MSEEIMSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIADYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWDWNPVFDKPIAPVGKVRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS
Homology
BLAST of Lag0009588 vs. NCBI nr
Match: XP_022140373.1 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Momordica charantia])

HSP 1 Score: 625.9 bits (1613), Expect = 2.7e-175
Identity = 311/473 (65.75%), Postives = 361/473 (76.32%), Query Frame = 0

Query: 10  NYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFP 69
           +YTAKVL H +ISPPAGSVP THLPL+F D ++I C P  R  FY FPHSTHHF+ AIFP
Sbjct: 7   DYTAKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIHAIFP 66

Query: 70  NLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIADYPR 129
            L+RSLSL+LQHFFPLAGNFI PP PH PHIL   HDSVPLTVAES+  DFD LIAD+PR
Sbjct: 67  TLKRSLSLALQHFFPLAGNFILPPPPHRPHILCTPHDSVPLTVAESSGCDFDRLIADHPR 126

Query: 130 DAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFM 189
            AK+FYP VPD+P +    DG  VVP+ ALQITVFPNQGLCI IAFHHVVADGMA   F+
Sbjct: 127 PAKHFYPLVPDLPNSRASPDGARVVPVFALQITVFPNQGLCISIAFHHVVADGMAAHDFL 186

Query: 190 KSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDKPI-- 249
           KS ASI+RNG   SGS DGPLPY D+A VKDPNG+ES+FLQ+LS WD  WNPV DKPI  
Sbjct: 187 KSXASIHRNGGG-SGSLDGPLPYHDRAAVKDPNGLESIFLQELSYWDSLWNPVPDKPIID 246

Query: 250 -APVGKVRATFVLTKTQIEKLKIWVSAR-------ESEPLRLSAFVVTCSLVWCCLIQTL 309
            AP  KVRATFV+ KTQIE+LK W+S +       E +PLRLSA +VTCSL+W CLI++ 
Sbjct: 247 LAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVTCSLIWGCLIKST 306

Query: 310 ESK--SNCFPDK-DEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGN 369
           E +   N  PD+ DE C+F+  ADCRNRL+DP +P TYFGNCLDM  V  K  +LL+  +
Sbjct: 307 EGEFAQNDLPDELDELCFFVLLADCRNRLKDP-LPPTYFGNCLDMGAVVLKKSELLKSNS 366

Query: 370 IGAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRP 429
               AKAIG RV+EFE EPLK ADKW SE  RC ES   + IAGSPKLR YDTDFGWG+P
Sbjct: 367 SVVAAKAIGKRVEEFESEPLKTADKWFSE-WRCLESKHLLTIAGSPKLRVYDTDFGWGKP 426

Query: 430 LKVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS 468
            K E+LH+D+S  ISL E RDE+GGIE+GLGL+K+HMDRF  +WEENL VLC+
Sbjct: 427 KKSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENLNVLCT 476

BLAST of Lag0009588 vs. NCBI nr
Match: XP_022971001.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 623.2 bits (1606), Expect = 1.8e-174
Identity = 300/471 (63.69%), Postives = 368/471 (78.13%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L    ISPP GSVP T LPL+F D ++  C P  R  F+QFPHST+HF++
Sbjct: 1   MADVDYTAKLLNLYWISPPPGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFID 60

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+ SLSLSLQ FFPLAGN I PP PH PHIL+  HDSVPLTVAEST GDF+ LIA
Sbjct: 61  SVFPTLKHSLSLSLQQFFPLAGNLILPPPPHKPHILYTPHDSVPLTVAESTGGDFNTLIA 120

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           D PR+AK+FYPFVPD+P++ +L DG+  +P  A+QIT+FPNQGLCICIAFHH VADGMA+
Sbjct: 121 DSPRNAKHFYPFVPDLPKSRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMAV 180

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASIYRNG  DS SF+GPLP  D+A VKDPNG+ES+FL +LS WD  WNPV +K
Sbjct: 181 HHFLKSWASIYRNG-GDSVSFEGPLPCHDRAAVKDPNGLESIFLHELSSWDSLWNPVPEK 240

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   APV KVR T VLT+  IEKLK WVSA     +E +PLRLS+F+VTCSLVW CLI+
Sbjct: 241 PIIDQAPVDKVRTTLVLTRPIIEKLKTWVSAQSNSDKELKPLRLSSFIVTCSLVWGCLIK 300

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +++++   D+DE CYF+  ADCRNRL+D  IP TYFGNCLD+  V  K  +LL+R +I
Sbjct: 301 STQNEASGSEDEDELCYFVLVADCRNRLKDQ-IPPTYFGNCLDVGAVVLKKSELLKRNSI 360

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
             VAKAIGNRV+EFE EP KMA++W S    C ++  F+++AGSPK R+YDTDFGWGRP 
Sbjct: 361 VVVAKAIGNRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRSYDTDFGWGRPK 420

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLC 467
           K E+LH+D+S  ISLHESRDEEGGIEIG+GL K+ MDR  T+WE+NLK LC
Sbjct: 421 KSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRLMTIWEQNLKGLC 469

BLAST of Lag0009588 vs. NCBI nr
Match: XP_023533558.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 621.3 bits (1601), Expect = 6.7e-174
Identity = 300/472 (63.56%), Postives = 369/472 (78.18%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L   +ISPPAGSVP T LPL+F D +++ C P  R  F+QFPHST+HFM+
Sbjct: 39  MADVDYTAKLLNLYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMD 98

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+RSLSLSLQHFFPLAGN I PPQPH PHIL+  HDSVPLTVAEST GDF  LIA
Sbjct: 99  SVFPTLKRSLSLSLQHFFPLAGNLILPPQPHKPHILYTPHDSVPLTVAESTGGDFTTLIA 158

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           DYPRDAK+FYPFVPD+PE+ +L DG+  +P  A+QIT+FPNQGLCICIAF HVVADG+AI
Sbjct: 159 DYPRDAKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIAI 218

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASI RNG   S SF+GPLP  ++A +KD  G+ES+FLQ+LS WD  WNPV  K
Sbjct: 219 HHFIKSWASISRNGGT-SDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPYK 278

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   APV +VR T VLT+  IEKLK WVSA      E +PLRLS+F+VTCSLVW CLI+
Sbjct: 279 PIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWGCLIK 338

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +S+++   D+DE CYF F ADCRNR++DP +P TYFGNCLD+  V+ K  +L++R +I
Sbjct: 339 STQSEASDSEDEDELCYFGFLADCRNRVKDP-LPPTYFGNCLDVGVVELKKSELVKRNSI 398

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
              AKAIGNRV+EFE EPLK+A+KW S      +   F+ IAGSPKL+AYDTDFGWGR +
Sbjct: 399 VMAAKAIGNRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAM 458

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS 468
           K E+LH+D S++ISL +SRDE+GGIEIGLGL  + MDRF  +WE+NLK LCS
Sbjct: 459 KSEVLHVDNSESISLQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLKDLCS 508

BLAST of Lag0009588 vs. NCBI nr
Match: KAG6604829.1 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.4 bits (1596), Expect = 2.6e-173
Identity = 298/471 (63.27%), Postives = 364/471 (77.28%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L    ISPPAGSVP T LPL+F D ++  C P  R  F+QFPHST+HF++
Sbjct: 1   MADVDYTAKLLNLYWISPPAGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFID 60

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+  LSLSLQ FFPLAGN I PP PH PHIL+  HDSVPLTV EST GDF+ LIA
Sbjct: 61  SVFPTLKHCLSLSLQQFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIA 120

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           D PRDAK+FYPFVPD+PE+ +L DG+  +P  A+QIT+FPNQGLCICIAFHH VADGMA+
Sbjct: 121 DSPRDAKHFYPFVPDLPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMAV 180

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASIYRNG  DS SF+GPLP  D+A VKDPNG+ES+F ++LS WD  WNPV  K
Sbjct: 181 HHFLKSWASIYRNG-GDSVSFEGPLPCHDRAAVKDPNGLESIFSRELSSWDSLWNPVPYK 240

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   AP+ KVR T VLT+  IEKLK WVSA     +E +PLRLS+F+VTCSLVW CLI+
Sbjct: 241 PIIDQAPIDKVRTTLVLTRPIIEKLKTWVSAESNSDKELKPLRLSSFIVTCSLVWGCLIK 300

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +S+++   D+DE CYF+  ADCRNRL+D  IP TYFGNCLD+  V  +  +LL+R + 
Sbjct: 301 STQSEASGSEDEDELCYFVLVADCRNRLKDQ-IPPTYFGNCLDVGAVVLRKSELLKRNST 360

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
             VAKAIG RV+EFE EP KMA++W S    C ++  F+++AGSPK R YDTDFGWGRP 
Sbjct: 361 AIVAKAIGKRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRTYDTDFGWGRPK 420

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLC 467
           K E+LH+D+S  ISLHESRDEEGGIEIG+GL K+ MDRF T+WE+NLK LC
Sbjct: 421 KSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRFMTIWEQNLKGLC 469

BLAST of Lag0009588 vs. NCBI nr
Match: XP_022947400.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 617.1 bits (1590), Expect = 1.3e-172
Identity = 297/472 (62.92%), Postives = 370/472 (78.39%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L   +ISPPAGSVP T LPL+F D +++ C P  R  F+QFPHST+HFM+
Sbjct: 39  MADVDYTAKLLNVYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMD 98

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+RSLSLSLQHFFPLAGN I PP PH PHIL+  HDSVPLTV EST GDF+ LIA
Sbjct: 99  SVFPTLKRSLSLSLQHFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIA 158

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           DYPRDAK+FYPFVPD+PE+ +L DG+  +P  A+QIT+FPNQGLCICIAF HVVADG+AI
Sbjct: 159 DYPRDAKHFYPFVPDMPESCLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIAI 218

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASI RNG A S SF+GPLP  ++A +KD  G+ES+FLQ+LS WD  WNPV  K
Sbjct: 219 HHFIKSWASISRNGGA-SDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPYK 278

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   APV +VR T VLT+  IEKLK WVSA      ES+PLRLS+F+VTCSLVW CLI+
Sbjct: 279 PIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEESKPLRLSSFIVTCSLVWGCLIK 338

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +S+++   D+D+ CYF F ADCRNR++DP +P TYFGNCLD+  V+ K  +L++R +I
Sbjct: 339 STQSEASDSVDEDKLCYFGFLADCRNRVKDP-LPPTYFGNCLDVGVVELKKSELVKRNSI 398

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
              AKAIG RV+EFE EPLK+A+KW S      +   F+ IAGSPKL+AYDTDFGWGR +
Sbjct: 399 VMAAKAIGKRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAM 458

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS 468
           K E+LH+D+S++IS+ +SRDE+GGIEIGLGL  + MDRF  +WE+NLK LCS
Sbjct: 459 KSEVLHVDSSESISVQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLKDLCS 508

BLAST of Lag0009588 vs. ExPASy Swiss-Prot
Match: Q9ZWB4 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=3AT1 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.4e-91
Identity = 184/460 (40.00%), Postives = 270/460 (58.70%), Query Frame = 0

Query: 14  KVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLER 73
           K+++   ISPP G+VP T LPLTFFDA +++ P      F+ + +ST  F++   PNL+ 
Sbjct: 9   KIIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 74  SLSLSLQHFFPLAGNFIFPPQPHNPHILFNT-HDSVPLTVAESTDGDFDHLIADYPRDAK 133
           SLS++LQHFFP AG  I PP+P  P++ +N   DS+  TVAEST+ DFD L +D P+D  
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRPDPPYLHYNDGQDSLVFTVAESTETDFDQLKSDSPKDIS 128

Query: 134 YFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKSW 193
             +  +P +P   V  +G  + P++A+Q+T+FP  G+CI  +  HVVADG+   HFMK W
Sbjct: 129 VLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYW 188

Query: 194 ASIYRNGAADSGSFDGP-LP-YLDKAFVKDPNGIESVFLQDLSGWDWNPV-FDKPIAPVG 253
            S+ ++   D  +   P LP +  +  +KDP  + +  L+    W  N       + P  
Sbjct: 189 MSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERF--WSQNSAKHSSHVTPEN 248

Query: 254 KVRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTL----ESKSNCFP 313
            VRATF L++ QI+ LK WV+ +      +S FVVT + +W  LI+TL    E+K+N   
Sbjct: 249 MVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLVQDSETKAN-EE 308

Query: 314 DKDEECYFLFTADCRNRLE-DPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGN 373
           DKDE  + +   DCRNRL+    IP TYFGNC+    V  K  DLL    + A + AI  
Sbjct: 309 DKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVLAASDAITA 368

Query: 374 RVKE-FEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHID 433
           R+K+    + LK A +W   + +   S    +IAG+PKL  YD DFG G+P K+E++HI+
Sbjct: 369 RIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIVHIE 428

Query: 434 TSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLK 464
           T  +I+  ESRD   G+EIG+ L K  MD F ++ ++ +K
Sbjct: 429 TGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIK 465

BLAST of Lag0009588 vs. ExPASy Swiss-Prot
Match: Q9LR73 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=3AT2 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.6e-88
Identity = 179/455 (39.34%), Postives = 263/455 (57.80%), Query Frame = 0

Query: 15  VLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLERS 74
           +++   ISPP G+V  T LPLTFFDA +++ P      F+ + +ST  F++   PNL+ S
Sbjct: 10  IIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKHS 69

Query: 75  LSLSLQHFFPLAGNFIFPPQPHNPHILFNT-HDSVPLTVAESTDGDFDHLIADYPRDAKY 134
           LS++LQHFFP AG  I PP+P  P++ +N   DS+  TVAEST+ DFD L +D P+D   
Sbjct: 70  LSITLQHFFPYAGKLIIPPRPDPPYLHYNAGEDSLVFTVAESTETDFDQLKSDSPKDISV 129

Query: 135 FYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKSWA 194
            +  +P +P   V  +G  + P++A+Q+T+FP  G+CI  +  HVVADG+   HFMK W 
Sbjct: 130 LHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYWM 189

Query: 195 SIYRNGAADSGSFDGP-LP-YLDKAFVKDPNGIESVFLQDLSGWDWNPV-FDKPIAPVGK 254
           S+ ++   D  +   P LP +  +  +KDP  + +  L+    W  N       + P   
Sbjct: 190 SLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERF--WSQNSAKHSSHVTPENM 249

Query: 255 VRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTLESKSNCFPDKDEE 314
           VRATF L++ QI+ LK WV+ +      +S FVVT + +W  LI+TL   S     KDE 
Sbjct: 250 VRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLVQDSET-EAKDEV 309

Query: 315 CYFLFTADCRNRLE-DPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGNRVKE- 374
            + +   DCRNRL+    IP TYFGNC+    V  K  DLL    + A + AI  R+K+ 
Sbjct: 310 FHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMAASDAITARIKDM 369

Query: 375 FEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHIDTSKTI 434
              + LK A +W   + +   S    +IAG+PKL  YD DFG G+P K+E++HI+T  +I
Sbjct: 370 LSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIVHIETGGSI 429

Query: 435 SLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLK 464
           +  ESRD   G+EIG+ L K  MD F ++ ++ +K
Sbjct: 430 AFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIK 461

BLAST of Lag0009588 vs. ExPASy Swiss-Prot
Match: Q589Y0 (Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.5e-75
Identity = 161/457 (35.23%), Postives = 254/457 (55.58%), Query Frame = 0

Query: 13  AKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLE 72
           A V++  Q+ P  GS     LPLT+FD V++A     R LFY+ P S   F++ I P L+
Sbjct: 2   ASVIEQCQVVPSPGSATELTLPLTYFDHVWLAFHRMRRILFYKLPISRPDFVQTIIPTLK 61

Query: 73  RSLSLSLQHFFPLAGNFIFPPQ-PHNPHILFNTHDSVPLTVAESTDGDFDHLIADYPRDA 132
            SLSL+L+++ PLAGN   P      P + + T +SV +  +ES D DF++LI  +PR+ 
Sbjct: 62  DSLSLTLKYYLPLAGNVACPQDWSGYPELRYVTGNSVSVIFSES-DMDFNYLIGYHPRNT 121

Query: 133 KYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKS 192
           K FY FVP + E      G  + P+LA+Q+T+FPN G+ I    HHV  DG  I  F+++
Sbjct: 122 KDFYHFVPQLAEPKDA-PGVQLAPVLAIQVTLFPNHGISIGFTNHHVAGDGATIVKFVRA 181

Query: 193 WASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWDWNPVFDKPIAPVGKV 252
           WA + + G  +    +  +P+ D++ +KDPNG+      ++  +         + P  KV
Sbjct: 182 WALLNKFGGDEQFLANEFIPFYDRSVIKDPNGVGMSIWNEMKKYKHMMKMSDVVTPPDKV 241

Query: 253 RATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTLESKSNCFPDKDEEC 312
           R TF++T+  I KLK  V  R  +   +++F VTC+ VW C+I++ E+ +    D++   
Sbjct: 242 RGTFIITRHDIGKLKNLVLTRRPKLTHVTSFTVTCAYVWTCIIKS-EAATGEEIDENGME 301

Query: 313 YFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGNRV-KEFE 372
           +F   ADCR +   P+ PS YFGN L     +++  DL  +       + IG  + K  +
Sbjct: 302 FFGCAADCRAQFNPPLPPS-YFGNALVGYVARTRQVDLAGKEGFTIAVELIGEAIRKRMK 361

Query: 373 REPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHIDTSK--TI 432
            E   ++  W  E  +  ++ R +++AGSPKL  Y  DFGWGRP K+E + ID     ++
Sbjct: 362 DEEWILSGSWFKEYDKV-DAKRSLSVAGSPKLDLYAADFGWGRPEKLEFVSIDNDDGISM 421

Query: 433 SLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVL 466
           SL +S+D +G +EIGL LSK+ M+ F  ++   +  L
Sbjct: 422 SLSKSKDSDGDLEIGLSLSKTRMNAFAAMFTHGISFL 453

BLAST of Lag0009588 vs. ExPASy Swiss-Prot
Match: Q9ZWR8 (Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.4e-75
Identity = 174/463 (37.58%), Postives = 258/463 (55.72%), Query Frame = 0

Query: 14  KVLKHSQISPPAGSVP-RTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLE 73
           KVL+  Q++PP+ +      LP+TFFD  ++        LFY FP+   HF++ + PNL+
Sbjct: 8   KVLEKCQVTPPSDTTDVELSLPVTFFDIPWLHLNKMQSLLFYDFPYPRTHFLDTVIPNLK 67

Query: 74  RSLSLSLQHFFPLAGNFIFP-PQPHNPHILFNTH--DSVPLTVAESTDGDFDHLIADYPR 133
            SLSL+L+H+ PL+GN + P      P   ++    DS+ L VAES D DFD+L      
Sbjct: 68  ASLSLTLKHYVPLSGNLLMPIKSGEMPKFQYSRDEGDSITLIVAES-DQDFDYLKGHQLV 127

Query: 134 DAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFM 193
           D+   +     +P          V+PL+A+Q+TVFPN+G+ + +  HH +AD  +   F+
Sbjct: 128 DSNDLHGLFYVMPRVIRTMQDYKVIPLVAVQVTVFPNRGIAVALTAHHSIADAKSFVMFI 187

Query: 194 KSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSG-WDWNPVFDKPIAPV 253
            +WA I + G          LP  D++ +KD  G+E  F  ++    +    F       
Sbjct: 188 NAWAYINKFGKDADLLSANLLPSFDRSIIKDLYGLEETFWNEMQDVLEMFSRFGSKPPRF 247

Query: 254 GKVRATFVLTKTQIEKLKIWV-SARESEP-LRLSAFVVTCSLVWCCLIQT-----LESKS 313
            KVRAT+VL+  +I+KLK  V + R SEP +R++ F +TC  VW C++++      E  S
Sbjct: 248 NKVRATYVLSLAEIQKLKNKVLNLRGSEPTIRVTTFTMTCGYVWTCMVKSKDDVVSEESS 307

Query: 314 NCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLL-ERGNIGAVAK 373
           N   D++E  YF FTADCR  L  P  P  YFGNCL  C  K+  K+L+ ++G + AVA 
Sbjct: 308 N---DENELEYFSFTADCRGLLTPP-CPPNYFGNCLASCVAKATHKELVGDKGLLVAVA- 367

Query: 374 AIGNRVKE---FEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKV 433
           AIG  +++    E+  L  A  WLSE      S RF+ I GSPK  +Y  DFGWG+P K 
Sbjct: 368 AIGEAIEKRLHNEKGVLADAKTWLSE-SNGIPSKRFLGITGSPKFDSYGVDFGWGKPAKF 427

Query: 434 EMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEE 461
           ++  +D ++ I + +SRD E G+EIG+ L K HMD F  ++EE
Sbjct: 428 DITSVDYAELIYVIQSRDFEKGVEIGVSLPKIHMDAFAKIFEE 463

BLAST of Lag0009588 vs. ExPASy Swiss-Prot
Match: Q8GSN8 (Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase OS=Dahlia pinnata OX=101596 GN=3MAT PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.3e-74
Identity = 166/461 (36.01%), Postives = 245/461 (53.15%), Query Frame = 0

Query: 15  VLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLERS 74
           +L+HS+ISPP  ++    LPLTFFD  ++  PP H   FY FP+S  HF E + PNL+ S
Sbjct: 9   ILEHSRISPPPSTIGHRSLPLTFFDIAWLLFPPVHHLYFYHFPYSKSHFTETVIPNLKHS 68

Query: 75  LSLSLQHFFPLAGNFIFPPQPHN----PHILFNTHDSVPLTVAESTDGDFDHLIADYPRD 134
           LS++LQH+FP  G  I  P PH+    P I     DSV LT AE+T  DF+ L A++PR 
Sbjct: 69  LSITLQHYFPFVGKLIVYPNPHDSTRKPEIRHVEGDSVALTFAETT-LDFNDLSANHPRK 128

Query: 135 AKYFYPFVPDVPEASVLHDGTHV-VPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFM 194
            + FYP VP  P  + + +  +V +P+ ++Q+T FPN G+ I +  HH ++D    F F+
Sbjct: 129 CENFYPLVP--PLGNAVKESDYVTLPVFSVQVTYFPNSGISIGLTNHHSLSDANTRFGFL 188

Query: 195 KSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWDWNPVFDKPIAPVG 254
           K+WAS+   G       +G  P  D+  V +P   E+   Q   G      F +  + VG
Sbjct: 189 KAWASVCETGEDQPFLKNGSPPVFDRVVV-NPQLYENRLNQTRLG-----TFYQAPSLVG 248

Query: 255 ----KVRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTLESKSNCFP 314
               +VRATFVL +T I  LK  V  +       S+F VTC  +W C++++L +      
Sbjct: 249 SSSDRVRATFVLARTHISGLKKQVLTQLPMLEYTSSFTVTCGYIWSCIVKSLVNMGE-KK 308

Query: 315 DKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGNR 374
            +DE   F+ +  CR+RL DP +P  YFGNC   C V  K   L         AK IG  
Sbjct: 309 GEDELEQFIVSVGCRSRL-DPPLPENYFGNCSAPCIVTIKNGVLKGENGFVMAAKLIGEG 368

Query: 375 VKEFEREP---LKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHI 434
           + +   +    L+ AD+W       +   R + I+G+PKL  YD DFGWG+ +K E++ I
Sbjct: 369 ISKMVNKKGGILEYADRWYDGF---KIPARKMGISGTPKLNFYDIDFGWGKAMKYEVVSI 428

Query: 435 DTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLK 464
           D S ++SL   ++     EIG+      M+ F  ++ + L+
Sbjct: 429 DYSASVSLSACKESAQDFEIGVCFPSMQMEAFGKIFNDGLE 455

BLAST of Lag0009588 vs. ExPASy TrEMBL
Match: A0A6J1CHV4 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111011063 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.3e-175
Identity = 311/473 (65.75%), Postives = 361/473 (76.32%), Query Frame = 0

Query: 10  NYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFP 69
           +YTAKVL H +ISPPAGSVP THLPL+F D ++I C P  R  FY FPHSTHHF+ AIFP
Sbjct: 7   DYTAKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIHAIFP 66

Query: 70  NLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIADYPR 129
            L+RSLSL+LQHFFPLAGNFI PP PH PHIL   HDSVPLTVAES+  DFD LIAD+PR
Sbjct: 67  TLKRSLSLALQHFFPLAGNFILPPPPHRPHILCTPHDSVPLTVAESSGCDFDRLIADHPR 126

Query: 130 DAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFM 189
            AK+FYP VPD+P +    DG  VVP+ ALQITVFPNQGLCI IAFHHVVADGMA   F+
Sbjct: 127 PAKHFYPLVPDLPNSRASPDGARVVPVFALQITVFPNQGLCISIAFHHVVADGMAAHDFL 186

Query: 190 KSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDKPI-- 249
           KS ASI+RNG   SGS DGPLPY D+A VKDPNG+ES+FLQ+LS WD  WNPV DKPI  
Sbjct: 187 KSXASIHRNGGG-SGSLDGPLPYHDRAAVKDPNGLESIFLQELSYWDSLWNPVPDKPIID 246

Query: 250 -APVGKVRATFVLTKTQIEKLKIWVSAR-------ESEPLRLSAFVVTCSLVWCCLIQTL 309
            AP  KVRATFV+ KTQIE+LK W+S +       E +PLRLSA +VTCSL+W CLI++ 
Sbjct: 247 LAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVTCSLIWGCLIKST 306

Query: 310 ESK--SNCFPDK-DEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGN 369
           E +   N  PD+ DE C+F+  ADCRNRL+DP +P TYFGNCLDM  V  K  +LL+  +
Sbjct: 307 EGEFAQNDLPDELDELCFFVLLADCRNRLKDP-LPPTYFGNCLDMGAVVLKKSELLKSNS 366

Query: 370 IGAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRP 429
               AKAIG RV+EFE EPLK ADKW SE  RC ES   + IAGSPKLR YDTDFGWG+P
Sbjct: 367 SVVAAKAIGKRVEEFESEPLKTADKWFSE-WRCLESKHLLTIAGSPKLRVYDTDFGWGKP 426

Query: 430 LKVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS 468
            K E+LH+D+S  ISL E RDE+GGIE+GLGL+K+HMDRF  +WEENL VLC+
Sbjct: 427 KKSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENLNVLCT 476

BLAST of Lag0009588 vs. ExPASy TrEMBL
Match: A0A6J1I239 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469804 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 8.6e-175
Identity = 300/471 (63.69%), Postives = 368/471 (78.13%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L    ISPP GSVP T LPL+F D ++  C P  R  F+QFPHST+HF++
Sbjct: 1   MADVDYTAKLLNLYWISPPPGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFID 60

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+ SLSLSLQ FFPLAGN I PP PH PHIL+  HDSVPLTVAEST GDF+ LIA
Sbjct: 61  SVFPTLKHSLSLSLQQFFPLAGNLILPPPPHKPHILYTPHDSVPLTVAESTGGDFNTLIA 120

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           D PR+AK+FYPFVPD+P++ +L DG+  +P  A+QIT+FPNQGLCICIAFHH VADGMA+
Sbjct: 121 DSPRNAKHFYPFVPDLPKSRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMAV 180

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASIYRNG  DS SF+GPLP  D+A VKDPNG+ES+FL +LS WD  WNPV +K
Sbjct: 181 HHFLKSWASIYRNG-GDSVSFEGPLPCHDRAAVKDPNGLESIFLHELSSWDSLWNPVPEK 240

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   APV KVR T VLT+  IEKLK WVSA     +E +PLRLS+F+VTCSLVW CLI+
Sbjct: 241 PIIDQAPVDKVRTTLVLTRPIIEKLKTWVSAQSNSDKELKPLRLSSFIVTCSLVWGCLIK 300

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +++++   D+DE CYF+  ADCRNRL+D  IP TYFGNCLD+  V  K  +LL+R +I
Sbjct: 301 STQNEASGSEDEDELCYFVLVADCRNRLKDQ-IPPTYFGNCLDVGAVVLKKSELLKRNSI 360

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
             VAKAIGNRV+EFE EP KMA++W S    C ++  F+++AGSPK R+YDTDFGWGRP 
Sbjct: 361 VVVAKAIGNRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRSYDTDFGWGRPK 420

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLC 467
           K E+LH+D+S  ISLHESRDEEGGIEIG+GL K+ MDR  T+WE+NLK LC
Sbjct: 421 KSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRLMTIWEQNLKGLC 469

BLAST of Lag0009588 vs. ExPASy TrEMBL
Match: A0A6J1G6S5 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451276 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 6.1e-173
Identity = 297/472 (62.92%), Postives = 370/472 (78.39%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L   +ISPPAGSVP T LPL+F D +++ C P  R  F+QFPHST+HFM+
Sbjct: 39  MADVDYTAKLLNVYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMD 98

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+RSLSLSLQHFFPLAGN I PP PH PHIL+  HDSVPLTV EST GDF+ LIA
Sbjct: 99  SVFPTLKRSLSLSLQHFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIA 158

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           DYPRDAK+FYPFVPD+PE+ +L DG+  +P  A+QIT+FPNQGLCICIAF HVVADG+AI
Sbjct: 159 DYPRDAKHFYPFVPDMPESCLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIAI 218

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASI RNG A S SF+GPLP  ++A +KD  G+ES+FLQ+LS WD  WNPV  K
Sbjct: 219 HHFIKSWASISRNGGA-SDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPYK 278

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   APV +VR T VLT+  IEKLK WVSA      ES+PLRLS+F+VTCSLVW CLI+
Sbjct: 279 PIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEESKPLRLSSFIVTCSLVWGCLIK 338

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +S+++   D+D+ CYF F ADCRNR++DP +P TYFGNCLD+  V+ K  +L++R +I
Sbjct: 339 STQSEASDSVDEDKLCYFGFLADCRNRVKDP-LPPTYFGNCLDVGVVELKKSELVKRNSI 398

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
              AKAIG RV+EFE EPLK+A+KW S      +   F+ IAGSPKL+AYDTDFGWGR +
Sbjct: 399 VMAAKAIGKRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAM 458

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS 468
           K E+LH+D+S++IS+ +SRDE+GGIEIGLGL  + MDRF  +WE+NLK LCS
Sbjct: 459 KSEVLHVDSSESISVQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLKDLCS 508

BLAST of Lag0009588 vs. ExPASy TrEMBL
Match: A0A6J1I4H8 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469803 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 1.0e-172
Identity = 298/472 (63.14%), Postives = 367/472 (77.75%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L   +ISPPAGSVP T LP++F D +++ C P  R  F+QFPHST+HFM+
Sbjct: 1   MADVDYTAKLLNLYRISPPAGSVPTTLLPISFLDFIWMICRPMRRLFFFQFPHSTNHFMD 60

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+ SLSLSLQHFFPLAGN I PPQP  PHIL+  HDSVPLTV EST GDF+ LIA
Sbjct: 61  SVFPTLKSSLSLSLQHFFPLAGNLILPPQPQKPHILYTPHDSVPLTVTESTGGDFNTLIA 120

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           DYPRDAK+FYPFVPD+PE+ +L DG+  +P  A+QIT+FPNQGLCICIAFHHVVADG+AI
Sbjct: 121 DYPRDAKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHVVADGIAI 180

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWD--WNPVFDK 245
            HF+KSWASI RNG   S SF+GPLP  ++A +KD  G+ES+FLQ+LS WD  WNPV  K
Sbjct: 181 HHFIKSWASISRNGGT-SDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPYK 240

Query: 246 PI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCCLIQ 305
           PI   APV +VR T VLT+  IEKLK WVSA      E +PLRLS+F+VTCSLVW CLI+
Sbjct: 241 PIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWGCLIK 300

Query: 306 TLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNI 365
           + +S+ +   D+DE C+F F ADCRNRL+DP +P TYFGNCLD+  V+ K  +L++R + 
Sbjct: 301 STQSEVSDSEDEDELCHFGFLADCRNRLKDP-LPPTYFGNCLDVGVVELKKSELVKRNST 360

Query: 366 GAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPL 425
              AKAIGNRV+EFE EPLK+A+KW S      +   F+ IAGSPKLRAYDTDFGWGR +
Sbjct: 361 VMAAKAIGNRVEEFENEPLKVAEKWFSLWKAYSKDKHFVTIAGSPKLRAYDTDFGWGRAM 420

Query: 426 KVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLCS 468
           K E+LH+D+S++ISL +SRDEEGGIEIGLGL  + MDRF  +WE+NLK LCS
Sbjct: 421 KSEVLHVDSSESISLQDSRDEEGGIEIGLGLHNTRMDRFMAIWEQNLKDLCS 470

BLAST of Lag0009588 vs. ExPASy TrEMBL
Match: A0A6J1G865 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451633 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.3e-172
Identity = 299/474 (63.08%), Postives = 364/474 (76.79%), Query Frame = 0

Query: 6   MSDSNYTAKVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFME 65
           M+D +YTAK+L    ISPPAGSVP T LPL+F D ++  C P  R  F+QFPHST+HF++
Sbjct: 1   MADVDYTAKLLNLYWISPPAGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFID 60

Query: 66  AIFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIA 125
           ++FP L+ SLSLSLQ FFPLAGN I PP PH PHIL+  HDSVPLTV EST GDF+ LIA
Sbjct: 61  SVFPTLKHSLSLSLQQFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIA 120

Query: 126 DYPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAI 185
           D PRDAK+FYPFVPD+PE+ +L DG+  +P  A+QIT+FPNQGLCICIAFHH VADGMA+
Sbjct: 121 DSPRDAKHFYPFVPDLPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMAV 180

Query: 186 FHFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQ---DLSGWD--WNPV 245
            HF+KSWASIYRNG  DS SF+GPLP  D+A VKDPNG+ES+F +    LS WD  WNPV
Sbjct: 181 HHFLKSWASIYRNG-GDSVSFEGPLPCHDRAAVKDPNGLESIFSRCEYSLSSWDSLWNPV 240

Query: 246 FDKPI---APVGKVRATFVLTKTQIEKLKIWVSA-----RESEPLRLSAFVVTCSLVWCC 305
             KPI   AP+ KVR T VLT+  IEKLK WVSA     +E +PLRLS+F+VTCSLVW C
Sbjct: 241 PYKPIIDQAPIDKVRTTLVLTRPIIEKLKTWVSAESNSDKELKPLRLSSFIVTCSLVWGC 300

Query: 306 LIQTLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLER 365
           LI++ +S+++   D+DE CYF+  ADCRNRL+D  IP TYFGNCLD+  V  +  +LL+R
Sbjct: 301 LIKSTQSEASGSEDEDELCYFVLVADCRNRLKDQ-IPPTYFGNCLDVGAVVLRKSELLKR 360

Query: 366 GNIGAVAKAIGNRVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWG 425
            +   VAKAIG RV+EFE EP KMA++W S    C ++  F+++AGSPK R YDTDFGWG
Sbjct: 361 NSTAIVAKAIGKRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRTYDTDFGWG 420

Query: 426 RPLKVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVLC 467
           RP K E+LH+D+S  ISLHESRDEEGGIEIG+GL K+ MDRF T+WE+NLK LC
Sbjct: 421 RPKKSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRFMTIWEQNLKGLC 472

BLAST of Lag0009588 vs. TAIR 10
Match: AT1G03940.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 335.9 bits (860), Expect = 5.3e-92
Identity = 184/460 (40.00%), Postives = 270/460 (58.70%), Query Frame = 0

Query: 14  KVLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLER 73
           K+++   ISPP G+VP T LPLTFFDA +++ P      F+ + +ST  F++   PNL+ 
Sbjct: 9   KIIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 74  SLSLSLQHFFPLAGNFIFPPQPHNPHILFNT-HDSVPLTVAESTDGDFDHLIADYPRDAK 133
           SLS++LQHFFP AG  I PP+P  P++ +N   DS+  TVAEST+ DFD L +D P+D  
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRPDPPYLHYNDGQDSLVFTVAESTETDFDQLKSDSPKDIS 128

Query: 134 YFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKSW 193
             +  +P +P   V  +G  + P++A+Q+T+FP  G+CI  +  HVVADG+   HFMK W
Sbjct: 129 VLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYW 188

Query: 194 ASIYRNGAADSGSFDGP-LP-YLDKAFVKDPNGIESVFLQDLSGWDWNPV-FDKPIAPVG 253
            S+ ++   D  +   P LP +  +  +KDP  + +  L+    W  N       + P  
Sbjct: 189 MSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERF--WSQNSAKHSSHVTPEN 248

Query: 254 KVRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTL----ESKSNCFP 313
            VRATF L++ QI+ LK WV+ +      +S FVVT + +W  LI+TL    E+K+N   
Sbjct: 249 MVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLVQDSETKAN-EE 308

Query: 314 DKDEECYFLFTADCRNRLE-DPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGN 373
           DKDE  + +   DCRNRL+    IP TYFGNC+    V  K  DLL    + A + AI  
Sbjct: 309 DKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVLAASDAITA 368

Query: 374 RVKE-FEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHID 433
           R+K+    + LK A +W   + +   S    +IAG+PKL  YD DFG G+P K+E++HI+
Sbjct: 369 RIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIVHIE 428

Query: 434 TSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLK 464
           T  +I+  ESRD   G+EIG+ L K  MD F ++ ++ +K
Sbjct: 429 TGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIK 465

BLAST of Lag0009588 vs. TAIR 10
Match: AT1G03495.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.9e-89
Identity = 179/455 (39.34%), Postives = 263/455 (57.80%), Query Frame = 0

Query: 15  VLKHSQISPPAGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHHFMEAIFPNLERS 74
           +++   ISPP G+V  T LPLTFFDA +++ P      F+ + +ST  F++   PNL+ S
Sbjct: 10  IIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKHS 69

Query: 75  LSLSLQHFFPLAGNFIFPPQPHNPHILFNT-HDSVPLTVAESTDGDFDHLIADYPRDAKY 134
           LS++LQHFFP AG  I PP+P  P++ +N   DS+  TVAEST+ DFD L +D P+D   
Sbjct: 70  LSITLQHFFPYAGKLIIPPRPDPPYLHYNAGEDSLVFTVAESTETDFDQLKSDSPKDISV 129

Query: 135 FYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKSWA 194
            +  +P +P   V  +G  + P++A+Q+T+FP  G+CI  +  HVVADG+   HFMK W 
Sbjct: 130 LHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYWM 189

Query: 195 SIYRNGAADSGSFDGP-LP-YLDKAFVKDPNGIESVFLQDLSGWDWNPV-FDKPIAPVGK 254
           S+ ++   D  +   P LP +  +  +KDP  + +  L+    W  N       + P   
Sbjct: 190 SLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERF--WSQNSAKHSSHVTPENM 249

Query: 255 VRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTLESKSNCFPDKDEE 314
           VRATF L++ QI+ LK WV+ +      +S FVVT + +W  LI+TL   S     KDE 
Sbjct: 250 VRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLVQDSET-EAKDEV 309

Query: 315 CYFLFTADCRNRLE-DPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAVAKAIGNRVKE- 374
            + +   DCRNRL+    IP TYFGNC+    V  K  DLL    + A + AI  R+K+ 
Sbjct: 310 FHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMAASDAITARIKDM 369

Query: 375 FEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHIDTSKTI 434
              + LK A +W   + +   S    +IAG+PKL  YD DFG G+P K+E++HI+T  +I
Sbjct: 370 LSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIVHIETGGSI 429

Query: 435 SLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLK 464
           +  ESRD   G+EIG+ L K  MD F ++ ++ +K
Sbjct: 430 AFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIK 461

BLAST of Lag0009588 vs. TAIR 10
Match: AT3G29635.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 242.3 bits (617), Expect = 7.9e-64
Identity = 170/474 (35.86%), Postives = 245/474 (51.69%), Query Frame = 0

Query: 14  KVLKHSQISPPAG----SVPRTHLPLTFFDAVFIACPPTHRFLFYQF--PHSTHHFMEAI 73
           KV K SQ+SP +     S     LPLTFFD  ++   PT R +FY+     S   F+  I
Sbjct: 4   KVTKISQVSPASNSSNDSANSMVLPLTFFDLRWLQFHPTERVIFYKLIKDSSLESFLSVI 63

Query: 74  FPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIADY 133
            P LE SLS+ L+H+ PLAG   +  Q   P I+ + +D V LTVAES D DF  +    
Sbjct: 64  LPKLELSLSIVLRHYLPLAGRLTWSSQDPKPSIIVSPNDYVSLTVAES-DADFSRISGKG 123

Query: 134 PRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFH 193
            R           VPE S+  D   V   L+LQ+T+FPNQG CI IA HH V DG  +  
Sbjct: 124 IRPESEIRSL---VPELSLSCDSPSV---LSLQVTLFPNQGFCIGIASHHSVMDGKTVVR 183

Query: 194 FMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWDWNPVFD----- 253
           F+KSWA I ++GA D    +   P LD+  +  P  +++  ++ LS   ++ V D     
Sbjct: 184 FIKSWAHICKHGAMDLP--EDLTPVLDRTVINVPASLDAKIIELLS--YFSEVKDSFRSL 243

Query: 254 -----KPIAPVGKVRATFVLTKTQIEKLKIWV---SARESEPLRLSAFVVTCSLVWCCLI 313
                K I+P   VR +  LT+  IEKL+      SAR    L LS FVV  + +W CL+
Sbjct: 244 KLLPPKEISP-DLVRISLELTRENIEKLREQAKRESARSHHELHLSTFVVANAYLWTCLV 303

Query: 314 QTLESKSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDM--CFVKSKMKDLLER 373
           +T         D++    F++ AD RNRL DP +P  YFGNC+    CF       L E 
Sbjct: 304 KTRGG------DENRPVRFMYAADFRNRL-DPPVPEMYFGNCVFPIGCFGYKANVFLGED 363

Query: 374 GNIGAVAKAIGNRVKEFEREPLK-MADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGW 433
           G +  V + + + V+      L+ + + +++     +   +  +IAGS +   Y +DFGW
Sbjct: 364 GFVNMV-EILSDSVRSIGLRKLETICELYINGTKSVKPGTQIGSIAGSNQFGLYGSDFGW 423

Query: 434 GRPLKVEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLKVL 466
           G+P   E+  ID ++  S+ E RDE GG+EIGL L K  MD F  +++  L+++
Sbjct: 424 GKPCNSEIASIDRNEAFSMSERRDEPGGVEIGLCLKKCEMDIFIYLFQNGLEMV 457

BLAST of Lag0009588 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 236.5 bits (602), Expect = 4.3e-62
Identity = 153/459 (33.33%), Postives = 230/459 (50.11%), Query Frame = 0

Query: 19  SQISPP-AGSVPRTHLPLTFFDAVFIACPPTHRFLFYQFPHSTHH-FMEAIFPNLERSLS 78
           S+++P  + S     LPLTFFD +++      R +FY+        F   I PNL+ SLS
Sbjct: 11  SRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIVPNLKSSLS 70

Query: 79  LSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIADYPRDAKYFYP 138
            SL H+ PLAG+ I+ P    P I++  +D+V  TVAES + DF  L    P  +   +P
Sbjct: 71  SSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAES-NSDFSLLTGKEPFSSTELHP 130

Query: 139 FVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIFHFMKSWASIY 198
            VP+      L +      +++ Q+T+FPNQG CI +  HH V+DG     F+KSWA + 
Sbjct: 131 LVPE------LQNSDDSAAVVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHLC 190

Query: 199 RNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGWDWNPV--------FDKPIAPV 258
           ++   DS   D  +P+ D+  +K P  I++  L+      W+ +          +P    
Sbjct: 191 KH--QDSSLPDDLIPFYDRTVIKGPPEIDTKVLK-----IWHSIHKPKSLKLLPRPEIES 250

Query: 259 GKVRATFVLTKTQIEKLKIWVSARES--EPLRLSAFVVTCSLVWCCLIQTLESKSNCFPD 318
             VR TF LT+  IEKL+  +    S    +RLS FV+T S V+ CLI       +   D
Sbjct: 251 DVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLI------GSGGDD 310

Query: 319 KDEECYFLFTADCRNRLEDPIIPSTYFGNCLDMCFVKSKMKDLLERGNIGAV--AKAIGN 378
            +    + F  DCR  ++DP IP TYFGNC+    VK  +   +  G  G V  A+ I +
Sbjct: 311 PNRPVGYRFAVDCRRLIDDPPIPLTYFGNCV-YSAVKIPLDAGMFLGEQGFVVAARLISD 370

Query: 379 RVKEFEREPLKMADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLKVEMLHIDT 438
            V+E +        + L    +     +F+++AGS +   Y  DFGWG+P K  ++ ID 
Sbjct: 371 SVEELDSNVAWKIPELLETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQ 430

Query: 439 SKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENLK 464
              IS+ ESRD  GG+EIG  L K  M+    +  + +K
Sbjct: 431 RGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK 448

BLAST of Lag0009588 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 228.8 bits (582), Expect = 9.0e-60
Identity = 153/466 (32.83%), Postives = 234/466 (50.21%), Query Frame = 0

Query: 12  TAKVLKHSQISPPAGSVPRT----HLPLTFFDAVFIACPPTHRFLFYQFPHSTH-HFMEA 71
           T  V++ ++++P   SV  +     LPLTFFD  ++   P  R  FY+   ST  HF   
Sbjct: 2   TLHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSI 61

Query: 72  IFPNLERSLSLSLQHFFPLAGNFIFPPQPHNPHILFNTHDSVPLTVAESTDGDFDHLIAD 131
           I P L+ SLSL L+++ PL G+  + P    P I+ + +  V +T+AES D DF HL   
Sbjct: 62  ILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAES-DADFSHLSGY 121

Query: 132 YPRDAKYFYPFVPDVPEASVLHDGTHVVPLLALQITVFPNQGLCICIAFHHVVADGMAIF 191
             R     +  VP +P +             ++QIT+FPNQG  I +A HH V DG    
Sbjct: 122 GQRPLSELHALVPKLPVSD------DSATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSS 181

Query: 192 HFMKSWASIYRNGAADSGSFDGPLPYLDKAFVKDPNGIESVFLQDLSGW--DWNPVFDKP 251
            F+K+WA I +         +   P  D++ +K P  ++   ++ +     D   +    
Sbjct: 182 TFIKAWAQICKQELQSMP--ENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLT 241

Query: 252 IAPVGK-----VRATFVLTKTQIEKLKIWVSARESEPLRLSAFVVTCSLVWCCLIQTLES 311
             P  K     V AT VL++  IE+L+  V    S  L LS FV+  +  W C ++    
Sbjct: 242 SLPSSKLGDDVVLATLVLSRADIERLREQVK-NVSPSLHLSTFVIAYAYAWTCFVKARGG 301

Query: 312 KSNCFPDKDEECYFLFTADCRNRLEDPIIPSTYFGNCLDM--CFVKSKMKDLLERGNIGA 371
                 +KD     LF  D R+RL DP +P TYFGNC+    C+ +   + + E+G + A
Sbjct: 302 ------NKDRSVSLLFVGDFRDRL-DPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTA 361

Query: 372 VAKAIGNRVKEFEREPLK-MADKWLSELMRCEESGRFINIAGSPKLRAYDTDFGWGRPLK 431
            A+ I + VK      ++ +AD ++        S +F  IAGS +L  Y+ DFGWGRP+K
Sbjct: 362 -AEIISDLVKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVK 421

Query: 432 VEMLHIDTSKTISLHESRDEEGGIEIGLGLSKSHMDRFTTVWEENL 463
           V+++ ID  + I++ E RDE GG+EIG+ L K+ MD   + +   L
Sbjct: 422 VDIVSIDQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGL 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140373.12.7e-17565.75LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltr... [more]
XP_022971001.11.8e-17463.69coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_023533558.16.7e-17463.56coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
KAG6604829.12.6e-17363.27Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1, partial ... [more]
XP_022947400.11.3e-17262.92coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
Match NameE-valueIdentityDescription
Q9ZWB47.4e-9140.00Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabid... [more]
Q9LR732.6e-8839.34Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabid... [more]
Q589Y01.5e-7535.23Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=... [more]
Q9ZWR84.4e-7537.58Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1[more]
Q8GSN86.3e-7436.01Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase OS=Dahlia ... [more]
Match NameE-valueIdentityDescription
A0A6J1CHV41.3e-17565.75LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltr... [more]
A0A6J1I2398.6e-17563.69coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1G6S56.1e-17362.92coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1I4H81.0e-17263.14coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1G8652.3e-17263.08coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
Match NameE-valueIdentityDescription
AT1G03940.15.3e-9240.00HXXXD-type acyl-transferase family protein [more]
AT1G03495.11.9e-8939.34HXXXD-type acyl-transferase family protein [more]
AT3G29635.17.9e-6435.86HXXXD-type acyl-transferase family protein [more]
AT5G39090.14.3e-6233.33HXXXD-type acyl-transferase family protein [more]
AT3G29670.19.0e-6032.83HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 19..456
e-value: 6.7E-43
score: 146.9
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 247..465
e-value: 4.0E-51
score: 175.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 12..238
e-value: 8.0E-65
score: 220.5
NoneNo IPR availablePANTHERPTHR31625FAMILY NOT NAMEDcoord: 13..466
NoneNo IPR availablePANTHERPTHR31625:SF52COUMAROYL-COA:ANTHOCYANIDIN 3-O-GLUCOSIDE-6''-O-COUMAROYLTRANSFERASE 1-RELATEDcoord: 13..466
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 103..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009588.1Lag0009588.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups