Lag0009325 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009325
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Phytocyanin domain-containing protein
Location	chr9: 38151987 .. 38154741 (-)
RNA-Seq Expression	Lag0009325
Synteny	Lag0009325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAATACGACAACGTTTCGAGATTATAAAAAAGTCAACAGAATCGGTGCAATGGGCCAGTCAACGCAGGAACACCCCATGTCAAGAGGCGCAGCTAATGGGCCTAAAGCCCATCAGGTTCGATCCGAATGTAATATACCAATTTATGCTATTCTTATAAATTATAGTTTAAATTAGAATTTTCAATCTTGGACAAAGTTTCAATTTCATCTCGATAAGTTTAAAATTTCAATTTAGTCTATGTAGTTAGAGTTGAGTTTCAATTTGGTCCCTATAATTTAATCAAATTTCACAAATTGTCACTTTATGATTGTGTTAGTTGACTAGATTGGAGTGAATTTTTCCTAGTAAGGAAAACACAATTTGGACGCAACTAGTAAGATTTAACCAAATTATAGAGACCAAATTGAAACCAGGTCCAAACGATAGGAACCAAACTGAAACTTTTAAAACTATAGAAACCAAACTGAAGTTAAATCTACAAGACTAAATTAAAACTCTTAAAACCACGAGAACCAAATTGAAAGAAATCGAATTTGTAACTTCTCTTTTCTTTTATTAATGGCACTGCACGTTTTACATAGATTTTAAAAGTCCTTTTAGATCTGTACTTCCTCTCAATAATCAACTCTACAATAGAAACCTATAATGAAACTCTCAAGTAATCATGACTAAGGAGAGACTTAATTAAACATTCATCTTCTATGATTTCTTCTTCTTGAAATGAACTGTTTGAGGCTGGAACTTCACCAAATACTGTTCAATTTAAACCATCTCCTTCCAAAACTGAAGCCTTTTTGAGCAACTTCCATTTAAACTTTCCCTGCTTAGGAATTGTTGTTTTGATATGGTCTTTGAGAATATTTTATAGCCTACAAGAATTTGCATAAATATTGACTTGTCAACAGAGCCAAGTCTTTATCTAACACTTGAGATTCAACAATTAAAAAACATTTGATATGCCTTATTTTTCAAACTGATCAATTTAGAGAAAGAGAAAGAGCTGTGATGGCCTTTTTCCCAGCTGTAACCACCTAAAACATGTTGATTGCAGTTTTGCTTCAAGATCAAATTTACTTAAATTCTCTTGTTTCTCTCTCTCTCTCTCTCTATAGGAAAAAGGAATTAGGAGTGAGAAACAATGATGGTGAAGTTGTATCATTGCAGATTGATCCATTTCTTCCTGTTTCTGAGTTATGGTGGAATGTCAATGTGTGAAGTCTTCCTTGTTGGAGATGAAGATGGCTGGAACTCTGGCATCAATTTTGCTACCTGGTCTCAAAGTCACAATTTCACCAAAGGAGATTTTCTGGGTATTCACTTCATTTTTCTTTTTCATTCACTATTTTATTGCAGTTTCTTCTTGAGTAATCTCATTATAAAAAAAAAAGTTAGTTTCTGGAATCAAATTAGTTGAATATTTGTGATTCTCCCATGAATATAACAGGATGAATTGAAAGATGAATGAATAGATATACAGAGAGAACCATGTATTTGTCTCTGTTAACTGTTTTACTTTTATGTCAATTAAATTAGCTCATGCCTAACTTTTCATGTTATATATCTACTTTGACACATGCCTAACCTTTCTTGATTTATTTGAAACTGTGATAACAAATTCACTTAGGAGAATTCATTTAGAATCGGTTACTTTAAGCCCTGTTTGATGACTATTTGGTTTTTGGTTTTTTTATTTTTAAAAATTAGGCTTGTTTTCTTTAAATTTCTTTACATGGTTTTCATTTTTGTTAAAGACAAACTTGAATTCCTAACGGAATTCTAAAAACAGAAACAAGTTTTGGTACTTTTTTTTAGTTTTAAAAAATTGGTTTTGTTTTTTAAAACATGGGAAGAAGGTAGATAAAAAAAAAAAAAAAAAACAAAGAAACTGAATCCTCGTTTGATAACCATTTGGATTTCGAAAATTATGTTTGTTTTCTCTCAAATTCCCTACAATGGTTTTCATCTTTATGCTAAGGATCAATTTGAATTCCTAGCCAAATTCCAAAAACAAAAAGGAAGTTTTTGAAAACTACTATTTTTTTTTTTTCAAAATTTGACTTGGTTTTTTAAAACAAAGAAGAAAGTAGATACTAAAACAAAGAAACTTATAGGTGGAAATAGATGTATATAAGCTTAATTTTAAAAAACAACCAAATGGTTATCAAATGGGGCCTTAGAATTGGAAACAATGTTTATAAGTTTAAAAACCAAATGGTTATCAAAAACGAAACTTTAAATATTGTTCAATTCAAACTACATAAACCAAACATGATTCATGTGCTCTAAGCAACTTCGACTATAACTTGGTTATGGATCGCTTCCAAATGACATAGCTAAACGACCCTTAACTCTTCGTTGACTATGATTAAGCTGTTGTTTCTCTCGATCTCGATTTTGTAGTTTTCAATTACGCGAAAAGTGTACACAATGTGGTCGAAGTTGTTGAGGAGACATATAGATCATGCAATACAAGTAATGGTGTATTGATTGAATATGAGAGCGGAAATGATAAGATTGAGCTGGAAGATGCAAGGAAGTATTGGTTCATTTGTAATGTGGATGGTCATTGCTTGGGTGGAATGAGGTTTGGAATTGTGGTCAAAGAAGCCAATTCTAGCATTCAAGAAGTCCCAATTAGTCCTTTTCAATCTCCTCCTCCCCCCACCACCAATAACCATGGTTCTAATGGAAGGTATCCAAGGTTGAGCACTTTGATTTTCATCATAGCATTTGAAATTCTATTTGGTTGA

mRNA sequence

ATGCAAATACGACAACGTTTCGAGATTATAAAAAAGTCAACAGAATCGGTGCAATGGGCCAGTCAACGCAGGAACACCCCATGTCAAGAGGCGCAGCTAATGGGCCTAAAGCCCATCAGATTGATCCATTTCTTCCTGTTTCTGAGTTATGGTGGAATGTCAATGTGTGAAGTCTTCCTTGTTGGAGATGAAGATGGCTGGAACTCTGGCATCAATTTTGCTACCTGGTCTCAAAGTCACAATTTCACCAAAGGAGATTTTCTGGTTTTCAATTACGCGAAAAGTGTACACAATGTGGTCGAAGTTGTTGAGGAGACATATAGATCATGCAATACAAGTAATGGTGTATTGATTGAATATGAGAGCGGAAATGATAAGATTGAGCTGGAAGATGCAAGGAAGTATTGGTTCATTTGTAATGTGGATGGTCATTGCTTGGGTGGAATGAGGTTTGGAATTGTGGTCAAAGAAGCCAATTCTAGCATTCAAGAAGTCCCAATTAGTCCTTTTCAATCTCCTCCTCCCCCCACCACCAATAACCATGGTTCTAATGGAAGGTATCCAAGGTTGAGCACTTTGATTTTCATCATAGCATTTGAAATTCTATTTGGTTGA

Coding sequence (CDS)

ATGCAAATACGACAACGTTTCGAGATTATAAAAAAGTCAACAGAATCGGTGCAATGGGCCAGTCAACGCAGGAACACCCCATGTCAAGAGGCGCAGCTAATGGGCCTAAAGCCCATCAGATTGATCCATTTCTTCCTGTTTCTGAGTTATGGTGGAATGTCAATGTGTGAAGTCTTCCTTGTTGGAGATGAAGATGGCTGGAACTCTGGCATCAATTTTGCTACCTGGTCTCAAAGTCACAATTTCACCAAAGGAGATTTTCTGGTTTTCAATTACGCGAAAAGTGTACACAATGTGGTCGAAGTTGTTGAGGAGACATATAGATCATGCAATACAAGTAATGGTGTATTGATTGAATATGAGAGCGGAAATGATAAGATTGAGCTGGAAGATGCAAGGAAGTATTGGTTCATTTGTAATGTGGATGGTCATTGCTTGGGTGGAATGAGGTTTGGAATTGTGGTCAAAGAAGCCAATTCTAGCATTCAAGAAGTCCCAATTAGTCCTTTTCAATCTCCTCCTCCCCCCACCACCAATAACCATGGTTCTAATGGAAGGTATCCAAGGTTGAGCACTTTGATTTTCATCATAGCATTTGAAATTCTATTTGGTTGA

Protein sequence

MQIRQRFEIIKKSTESVQWASQRRNTPCQEAQLMGLKPIRLIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANSSIQEVPISPFQSPPPPTTNNHGSNGRYPRLSTLIFIIAFEILFG

Homology

[Close]

BLAST of Lag0009325 vs. NCBI nr
Match: XP_004147051.2 (blue copper protein isoform X1 [Cucumis sativus] >KAE8651903.1 hypothetical protein Csa_006334 [Cucumis sativus])

HSP 1 Score: 280.0 bits (715), Expect = 1.6e-71
Identity = 138/191 (72.25%), Postives = 158/191 (82.72%), Query Frame = 0


Query: 17  VQWASQRRNTPCQEAQLMGLKPIRLIH-FFLFLSYGGMSMCEVFLVGDEDGWNSGINFAT 76
           +QWA+QRR +PC + QLMGLKPIRLIH FFLFLS+ G+SMCEVF+VGDEDGWNSG NFAT
Sbjct: 1   MQWATQRRYSPCPQPQLMGLKPIRLIHYFFLFLSFFGVSMCEVFVVGDEDGWNSGTNFAT 60

Query: 77  WSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKY 136
           WSQSHNFTKGDFLVFNYAK+VHNV EV+EETYRSC   NGVL EY+SGNDKIEL++AR Y
Sbjct: 61  WSQSHNFTKGDFLVFNYAKNVHNVYEVIEETYRSCEAKNGVLGEYDSGNDKIELKEARNY 120

Query: 137 WFICNVDGHCLGGMRFGIVVKEANSSIQEVPISPFQSPPPPTTNNHGS--NGRYPRLST- 196
           WFICNV GHCLGGMRFGIVVKE+NSS   +P++P    PPP T NH S   GR+P   T 
Sbjct: 121 WFICNVAGHCLGGMRFGIVVKESNSS-THLPLNPIDQSPPPNT-NHASICCGRFPMWWTF 180

Query: 197 LIFIIAFEILF 204
            I I++F +LF
Sbjct: 181 FICILSFNVLF 189

[Close]

BLAST of Lag0009325 vs. NCBI nr
Match: XP_031736432.1 (chemocyanin isoform X2 [Cucumis sativus])

HSP 1 Score: 259.2 bits (661), Expect = 2.9e-65
Identity = 129/190 (67.89%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 17  VQWASQRRNTPCQEAQLMGLKPIRLIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATW 76
           +QWA+QRR +PC + QLMGLKPI         S+ G+SMCEVF+VGDEDGWNSG NFATW
Sbjct: 1   MQWATQRRYSPCPQPQLMGLKPI---------SFFGVSMCEVFVVGDEDGWNSGTNFATW 60

Query: 77  SQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYW 136
           SQSHNFTKGDFLVFNYAK+VHNV EV+EETYRSC   NGVL EY+SGNDKIEL++AR YW
Sbjct: 61  SQSHNFTKGDFLVFNYAKNVHNVYEVIEETYRSCEAKNGVLGEYDSGNDKIELKEARNYW 120

Query: 137 FICNVDGHCLGGMRFGIVVKEANSSIQEVPISPFQSPPPPTTNNHGS--NGRYPRLST-L 196
           FICNV GHCLGGMRFGIVVKE+NSS   +P++P    PPP T NH S   GR+P   T  
Sbjct: 121 FICNVAGHCLGGMRFGIVVKESNSS-THLPLNPIDQSPPPNT-NHASICCGRFPMWWTFF 179

Query: 197 IFIIAFEILF 204
           I I++F +LF
Sbjct: 181 ICILSFNVLF 179

[Close]

BLAST of Lag0009325 vs. NCBI nr
Match: KAG6571015.1 (hypothetical protein SDJN03_29930, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010846.1 hypothetical protein SDJN02_27642, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 256.9 bits (655), Expect = 1.5e-64
Identity = 124/164 (75.61%), Postives = 140/164 (85.37%), Query Frame = 0


Query: 41  LIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVV 100
           ++  F+FLS+GG+SMCEVFLVGDE+GWNSGINFATWSQ+HNFTKGDFLVFNYAKSVHNV 
Sbjct: 3   MVKLFMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVY 62

Query: 101 EVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANS 160
           EV EET+RSC TSNGVL EYESGND+IEL++ARKYWFICNV GHCLGGMRFGI VK+ NS
Sbjct: 63  EVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVKQPNS 122

Query: 161 SIQEVPISPFQSPPPPTTNNHGSN-GRYPRLSTLIFIIAFEILF 204
           S   +P+SP QSPPPPT  NH SN G Y   ST + IIAF+ LF
Sbjct: 123 S-SHLPLSPVQSPPPPT--NHASNHGSYALWSTFMCIIAFQTLF 163

[Close]

BLAST of Lag0009325 vs. NCBI nr
Match: XP_022943841.1 (basic blue protein-like [Cucurbita moschata])

HSP 1 Score: 256.1 bits (653), Expect = 2.5e-64
Identity = 124/164 (75.61%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 41  LIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVV 100
           ++  F+FLS+GG+SMCEVFLVGDE+GWNSGINFATWSQ+HNFTKGDFLVFNYAKSVHNV 
Sbjct: 3   MVKLFMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVY 62

Query: 101 EVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANS 160
           EV EET+RSC TSNGVL EYESGND+IEL++ARKYWFICNV GHCLGGMRFGI V EANS
Sbjct: 63  EVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVNEANS 122

Query: 161 SIQEVPISPFQSPPPPTTNNHGSN-GRYPRLSTLIFIIAFEILF 204
               +P+SP QSPPPPT  NH SN G Y   ST + IIAF+ LF
Sbjct: 123 R-SHLPLSPVQSPPPPT--NHASNHGSYALWSTFMCIIAFQTLF 163

[Close]

BLAST of Lag0009325 vs. NCBI nr
Match: XP_023513085.1 (basic blue protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.4 bits (646), Expect = 1.6e-63
Identity = 123/164 (75.00%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 41  LIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVV 100
           ++   +FLS+GG+SMCEVFLVGDE+GWNSGINFATWSQ+HNFTKGDFLVFNYAKSVHNV 
Sbjct: 5   MVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVY 64

Query: 101 EVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANS 160
           EV EET+RSC TSNGVL EYESGND+IEL++ARKYWFICNV GHCLGGMRFGI VK+ NS
Sbjct: 65  EVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVKQPNS 124

Query: 161 SIQEVPISPFQSPPPPTTNNHGSN-GRYPRLSTLIFIIAFEILF 204
           +   +P SP QSPPPPT  NHGSN G Y   ST + IIAF+ LF
Sbjct: 125 T-SHLPPSPVQSPPPPT--NHGSNHGSYAIWSTFMCIIAFQTLF 165

[Close]

BLAST of Lag0009325 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.3e-19
Identity = 47/119 (39.50%), Postives = 65/119 (54.62%), Query Frame = 0


Query: 54  SMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTS 113
           S+  V+ VGD  GW  G +++TW+    F  GD LVFNY    H V EV E  Y+SC + 
Sbjct: 22  SLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAGAHTVDEVKESDYKSCTSG 81

Query: 114 NGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANSSIQEVPISPFQS 173
           N +  +  +G   I L+ A K++FIC V GH  GGM+  I VK ++ S      +P  S
Sbjct: 82  NSISTD-STGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASSGSSAAPSATPSSS 139

[Close]

BLAST of Lag0009325 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.4e-15
Identity = 41/106 (38.68%), Postives = 62/106 (58.49%), Query Frame = 0


Query: 58  VFLVGDEDGWNSGI--NFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNG 117
           V  VGD  GW + +  ++A W+ S+ F  GD L+FNY    HNV++V +E ++SCN+S+ 
Sbjct: 3   VHKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSSSP 62

Query: 118 VLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANSS 162
               Y SG D I L+    ++F+C + GHC  G +  I V   +SS
Sbjct: 63  A-ASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEIKVDPGSSS 107

[Close]

BLAST of Lag0009325 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 47/144 (32.64%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 34  MGLKPIRLIHFFLFLSYGGMSMCEVFLVGDEDGW----NSGINFATWSQSHNFTKGDFLV 93
           M    + +  F L   +   +  EV + G    W    +S  +F  W+Q   F  GDF+V
Sbjct: 7   MASSSLHVAIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIV 66

Query: 94  FNYAKSVHNVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGM 153
           F Y     +V+EV +E Y SCNT+N  L  Y  G  K++L+ +  ++FI   +GHC  G 
Sbjct: 67  FRYESGKDSVLEVTKEAYNSCNTTN-PLANYTDGETKVKLDRSGPFYFISGANGHCEKGQ 126

Query: 154 RFGIVVKEANSSIQEVPISPFQSP 174
           +  +VV     S+    ISP  SP
Sbjct: 127 KLSLVVISPRHSV----ISPAPSP 145

[Close]

BLAST of Lag0009325 vs. ExPASy Swiss-Prot
Match: P60496 (Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 7.1e-14
Identity = 41/96 (42.71%), Postives = 54/96 (56.25%), Query Frame = 0


Query: 58  VFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVL 117
           V+ VGD  GW  G   + W     F  GD LVF Y  +VHNVV V    Y+SC  S G  
Sbjct: 32  VYTVGDGGGWTFGT--SGWPAGKTFRAGDVLVFKYNPAVHNVVSVPAGGYKSCTASPGSR 91

Query: 118 IEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGI 154
           + ++SG+D+I L     Y FIC+V GHC GG++  +
Sbjct: 92  V-FKSGDDRITLSRGTNY-FICSVPGHCQGGLKIAV 123

[Close]

BLAST of Lag0009325 vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 9.3e-14
Identity = 39/96 (40.62%), Postives = 54/96 (56.25%), Query Frame = 0


Query: 58  VFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVL 117
           V++VG   GW    N  +W +   F  GD L+FNY   +HNVV V +  + +CNT  G  
Sbjct: 2   VYVVGGSGGWT--FNTESWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGAK 61

Query: 118 IEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGI 154
           + Y SG D+I+L   + Y FICN  GHC  GM+  +
Sbjct: 62  V-YTSGRDQIKLPKGQSY-FICNFPGHCQSGMKIAV 93

[Close]

BLAST of Lag0009325 vs. ExPASy TrEMBL
Match: A0A6J1FST3 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448452 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.2e-64
Identity = 124/164 (75.61%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 41  LIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVV 100
           ++  F+FLS+GG+SMCEVFLVGDE+GWNSGINFATWSQ+HNFTKGDFLVFNYAKSVHNV 
Sbjct: 3   MVKLFMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVY 62

Query: 101 EVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANS 160
           EV EET+RSC TSNGVL EYESGND+IEL++ARKYWFICNV GHCLGGMRFGI V EANS
Sbjct: 63  EVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVNEANS 122

Query: 161 SIQEVPISPFQSPPPPTTNNHGSN-GRYPRLSTLIFIIAFEILF 204
               +P+SP QSPPPPT  NH SN G Y   ST + IIAF+ LF
Sbjct: 123 R-SHLPLSPVQSPPPPT--NHASNHGSYALWSTFMCIIAFQTLF 163

[Close]

BLAST of Lag0009325 vs. ExPASy TrEMBL
Match: A0A6J1JF60 (basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483949 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.2e-61
Identity = 118/164 (71.95%), Postives = 137/164 (83.54%), Query Frame = 0


Query: 41  LIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVV 100
           ++   +FLS+GG+SMCEVFLVGDE+GWNSGINFATWSQ+HNFTKGDFLVFNYAKSVHNV 
Sbjct: 5   MVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVY 64

Query: 101 EVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANS 160
           EV EET+RSC TSNGVL EYESGND+IEL++ARKYWFICNV GHCLGGMRFGI V EAN 
Sbjct: 65  EVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANP 124

Query: 161 SIQEVPISPFQSPPPPTTNNHGS-NGRYPRLSTLIFIIAFEILF 204
               +P+SP QSPPPPT  NH S +G Y   S+ + ++AF+ LF
Sbjct: 125 R-SHLPLSPVQSPPPPT--NHASHHGSYALWSSFMGMVAFQTLF 165

[Close]

BLAST of Lag0009325 vs. ExPASy TrEMBL
Match: A0A1S3C699 (mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103497341 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 4.9e-58
Identity = 111/159 (69.81%), Postives = 126/159 (79.25%), Query Frame = 0


Query: 41  LIHFFLFLSYGGMSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVV 100
           + +FFLFLS+ G+SMCEVF+VGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAK+VHNV 
Sbjct: 12  IYYFFLFLSFFGVSMCEVFVVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKNVHNVY 71

Query: 101 EVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANS 160
           EV+EETYRSC   NGVL EY+SGNDKIEL++ R YWFICNV GHCLGGMRFGI VK+  +
Sbjct: 72  EVIEETYRSCEAKNGVLGEYDSGNDKIELKEGRNYWFICNVVGHCLGGMRFGIEVKQQPN 131

Query: 161 SIQEVPISPFQSPPPPTTNNHGS--NGRYPRLSTLIFII 198
           S   +P++P    PPP T NH S   GRYP   T    I
Sbjct: 132 SSTHLPLNPIDQSPPPNT-NHASICCGRYPMWWTFFICI 169

[Close]

BLAST of Lag0009325 vs. ExPASy TrEMBL
Match: A0A6J1CYL0 (basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111015378 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 7.1e-57
Identity = 120/167 (71.86%), Postives = 136/167 (81.44%), Query Frame = 0


Query: 41  LIHF-FLFLSYGG--MSMCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVH 100
           LIH+ FLFLS+GG  +S CEVFLVG EDGWNSGINF +WSQ+HNFTKGD LVFNY+K+ H
Sbjct: 10  LIHYLFLFLSFGGILLSKCEVFLVGGEDGWNSGINFVSWSQTHNFTKGDVLVFNYSKNEH 69

Query: 101 NVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKE 160
           NV EV EETYRSCN S GV+ EY+SGND+IEL++ARKYWFICNV GHCLGGMRFGI V +
Sbjct: 70  NVYEVSEETYRSCNASKGVVGEYDSGNDEIELKEARKYWFICNVAGHCLGGMRFGIEVNQ 129

Query: 161 ANSSIQEVPISPFQSPPPPTTNNHGSNGRYP-RLSTLIFIIAFEILF 204
            NS+I  VP S FQ PPPPTT NHGS   Y    STLIFIIAF ++F
Sbjct: 130 PNSTI-HVPNSDFQ-PPPPTT-NHGSYESYQIWSSTLIFIIAFGLVF 173

[Close]

BLAST of Lag0009325 vs. ExPASy TrEMBL
Match: A0A0A0LME3 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270850 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.3e-55
Identity = 110/152 (72.37%), Postives = 124/152 (81.58%), Query Frame = 0


Query: 55  MCEVFLVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSN 114
           MCEVF+VGDEDGWNSG NFATWSQSHNFTKGDFLVFNYAK+VHNV EV+EETYRSC   N
Sbjct: 1   MCEVFVVGDEDGWNSGTNFATWSQSHNFTKGDFLVFNYAKNVHNVYEVIEETYRSCEAKN 60

Query: 115 GVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANSSIQEVPISPFQSPP 174
           GVL EY+SGNDKIEL++AR YWFICNV GHCLGGMRFGIVVKE+NSS   +P++P    P
Sbjct: 61  GVLGEYDSGNDKIELKEARNYWFICNVAGHCLGGMRFGIVVKESNSS-THLPLNPIDQSP 120

Query: 175 PPTTNNHGS--NGRYPRLST-LIFIIAFEILF 204
           PP T NH S   GR+P   T  I I++F +LF
Sbjct: 121 PPNT-NHASICCGRFPMWWTFFICILSFNVLF 150

[Close]

BLAST of Lag0009325 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.2e-18
Identity = 47/143 (32.87%), Postives = 74/143 (51.75%), Query Frame = 0


Query: 53  MSMCEVFLVGDEDGWN--SGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSC 112
           +S   V+ VGD  GW   + +++  W+ +  F  GD ++F Y    HNV+ V    YRSC
Sbjct: 18  LSEAAVYKVGDSAGWTTIANVDYKLWASTKTFHIGDTVLFEYNPQFHNVMRVTHPMYRSC 77

Query: 113 NTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGMRFG--IVVKEANSSIQEVPIS 172
           NTS  +   + +GND I L +   ++F C V GHCL G +    +++  +++ + + P S
Sbjct: 78  NTSKPI-STFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLHVLLPASSTPLSDPPTS 137

Query: 173 PFQSPPPPTTNNHGSNGRYPRLS 192
              SPP  T    G  G  P L+
Sbjct: 138 SSSSPPSTTIPAAGVPGPSPSLA 159

[Close]

BLAST of Lag0009325 vs. TAIR 10
Match: AT3G17675.1 (Cupredoxin superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.7e-18
Identity = 40/96 (41.67%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 60  LVGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVLIE 119
           +VGD +GW    N+  W+Q   F  GD LVFNY    HNV++V    Y  C   N   + 
Sbjct: 10  IVGDSNGWELFTNYTNWTQGREFHVGDVLVFNYKSDQHNVMQVNSTAYTDCGLDNYTTL- 69

Query: 120 YESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVV 156
           +  GND I L +  K WFIC VD HC+ G +  I V
Sbjct: 70  FTKGNDSIILSEVGKLWFICGVDDHCVNGQKLSINV 104

[Close]

BLAST of Lag0009325 vs. TAIR 10
Match: AT2G25060.1 (early nodulin-like protein 14 )

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-15
Identity = 47/144 (32.64%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 34  MGLKPIRLIHFFLFLSYGGMSMCEVFLVGDEDGW----NSGINFATWSQSHNFTKGDFLV 93
           M    + +  F L   +   +  EV + G    W    +S  +F  W+Q   F  GDF+V
Sbjct: 7   MASSSLHVAIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIV 66

Query: 94  FNYAKSVHNVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGM 153
           F Y     +V+EV +E Y SCNT+N  L  Y  G  K++L+ +  ++FI   +GHC  G 
Sbjct: 67  FRYESGKDSVLEVTKEAYNSCNTTN-PLANYTDGETKVKLDRSGPFYFISGANGHCEKGQ 126

Query: 154 RFGIVVKEANSSIQEVPISPFQSP 174
           +  +VV     S+    ISP  SP
Sbjct: 127 KLSLVVISPRHSV----ISPAPSP 145

[Close]

BLAST of Lag0009325 vs. TAIR 10
Match: AT3G01070.1 (early nodulin-like protein 16 )

HSP 1 Score: 75.9 bits (185), Expect = 4.3e-14
Identity = 41/127 (32.28%), Postives = 62/127 (48.82%), Query Frame = 0


Query: 61  VGDEDGWNSGINFATWSQSHNFTKGDFLVFNYAKSVHNVVEVVEETYRSCNTSNGVL-IE 120
           VGD   WN  IN+  W+Q  +F   D+L F Y ++ +NV+EV E  Y SCN +N +    
Sbjct: 29  VGDNKFWNPNINYTIWAQDKHFYLDDWLYFVYERNQYNVIEVNETNYISCNPNNPIANWS 88

Query: 121 YESGNDKIELEDARKYWFICNVDGHCLGGMRFGIVVKEANSSIQEVPISPFQSPPPPTTN 180
             +G D + L   R Y+ I    G C GGM+  ++V+              + PPPP   
Sbjct: 89  RGAGRDLVHLNVTRHYYLISGNGGGCYGGMKLAVLVE--------------KPPPPPAAA 141

Query: 181 NHGSNGR 187
            + ++ R
Sbjct: 149 PNKNSAR 141

[Close]

BLAST of Lag0009325 vs. TAIR 10
Match: AT4G30590.1 (early nodulin-like protein 12 )

HSP 1 Score: 75.1 bits (183), Expect = 7.3e-14
Identity = 50/156 (32.05%), Postives = 77/156 (49.36%), Query Frame = 0


Query: 38  PIRLIHFFLFLSYG-GMSMCEVFLVGDEDG-W------NSGINFATWSQSHNFTKGDFLV 97
           P+  + F LF     G S   V LVG   G W      N+ +N   W++++ F  GDF+V
Sbjct: 6   PVLTLVFLLFAKVSHGASNPRVILVGGSVGSWKVPDSPNNTLNH--WAENNRFKVGDFIV 65

Query: 98  FNYAKSVHNVVEVVEETYRSCNTSNGVLIEYESGNDKIELEDARKYWFICNVDGHCLGGM 157
           + Y   V +V++V +E Y SCNT+N  L +Y  GN K+ L+ +  Y+FI    G+C  G 
Sbjct: 66  WKYDMKVDSVLQVTKEDYESCNTAN-PLKQYNDGNTKVALDKSGPYFFISGAPGNCAKGE 125

Query: 158 RFGIVV---------KEANSSIQEVPISPFQSPPPP 177
           +  +VV           +  + +  P+SP    P P
Sbjct: 126 KITLVVLAERKSGGGSSSGDAPKVSPVSPTAQTPAP 158

The following BLAST results are available for this feature:

BLAST of Lag0009325 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147051.2	1.6e-71	72.25	blue copper protein isoform X1 [Cucumis sativus] >KAE8651903.1 hypothetical prot...	[more]
XP_031736432.1	2.9e-65	67.89	chemocyanin isoform X2 [Cucumis sativus]	[more]
KAG6571015.1	1.5e-64	75.61	hypothetical protein SDJN03_29930, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022943841.1	2.5e-64	75.61	basic blue protein-like [Cucurbita moschata]	[more]
XP_023513085.1	1.6e-63	75.00	basic blue protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0009325 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41001	7.3e-19	39.50	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P80728	6.4e-15	38.68	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
Q9SK27	1.4e-14	32.64	Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S...	[more]
P60496	7.1e-14	42.71	Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1	[more]
P00303	9.3e-14	40.63	Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]

BLAST of Lag0009325 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FST3	1.2e-64	75.61	basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448452 PE=4 SV=1	[more]
A0A6J1JF60	1.2e-61	71.95	basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483949 PE=4 SV=1	[more]
A0A1S3C699	4.9e-58	69.81	mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103497341 PE=4 SV=1	[more]
A0A6J1CYL0	7.1e-57	71.86	basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111015378 PE=4 SV=1	[more]
A0A0A0LME3	2.3e-55	72.37	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270850...	[more]

BLAST of Lag0009325 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26330.1	2.2e-18	32.87	Cupredoxin superfamily protein	[more]
AT3G17675.1	3.7e-18	41.67	Cupredoxin superfamily protein	[more]
AT2G25060.1	1.0e-15	32.64	early nodulin-like protein 14	[more]
AT3G01070.1	4.3e-14	32.28	early nodulin-like protein 16	[more]
AT4G30590.1	7.3e-14	32.05	early nodulin-like protein 12	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 55..162 e-value: 1.6E-31 score: 110.6
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 57..156
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 67..149 e-value: 5.4E-23 score: 80.8
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 57..157 score: 33.956108
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 165..184
None	No IPR available	PANTHER	PTHR33021:SF179	BLUE COPPER BINDING PROTEIN-LIKE	coord: 40..159
None	No IPR available	CDD	cd04216	Phytocyanin	coord: 58..155 e-value: 1.83694E-36 score: 121.213
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 40..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009325.1	Lag0009325.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity