Lag0009306 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009306
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPoly(A)-specific ribonuclease
Locationchr9: 37989460 .. 37990459 (-)
RNA-Seq ExpressionLag0009306
SyntenyLag0009306
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGAACACGATGAGCAGAGAGAATCGATGGGGTCCGAGAAGACGAAAACCAATTATTCGAGAGGTCTGGGCTTGGAATTTCGAGTCTGAAATCGCCCGCTTCGATCGCTGCCTTGAATCGTACGCGATTCTGTCGATCGACACCGAGTTTCCTGGGTGTATTCAGACTGCTCCTTGGGGCGCCATCGACGAACAAGTTTATCAGAATATGAGGGTCAATGTAAATCAGACGAAGCTGATTCAACTGGGTCTGACTGCCTCCGATGAATGCGGGAAGATCGGCGGCGCCTGGGAGTTTAATTTCTGTGATTTCGACGCCGAAACCGACGCCCACAACCCTTCGTCCATTTCGTTCCTGAAACAGAACGGCCTGGATTTCGACCGATTCAAATCCGACGGGATTCCACTGGTTGCATTCACGGCGATTTTCGTTCAAATCTTACGAAAGAACCAAATCTTCCGATGGGTCACATTTCACGGCCTGTACGATATCGGGTAAGTAGAAATTAAATTTCACCGCCGATTTCCAATTCCATTTGGTTTCGATTCACTGAATTGTTGTTTTCCTCAACAGATACCTCTTGAAGGCGATGGGGGTGGTCGAGGAGTTGCCGGAATCAATTGAGGATTTCGGGAGTTTAGTGGTGAATGCGGTAGGCAGTGTGCGCGATCTGAAGCACATTGCTCGGTTCTGTGAAGGGCTGGAAGATGGGAAATTCGGCCTGGAAAGACTGGGCGAACTGCTCGAGCAAAAGCGGTTCGGATCGAAGCACCATGCCGGCTCAGACAGTCTGCTGACGGCTGCACTGCACACGAGGATGATGGAACGGTTTGAACCGGATTCGGAAATCTGCGACGGGTTTCTGTATGGGCTTACAAACAAACTCCAAAACATGGAGATGATGAGGATGAAGGCGGTTCAGACCATAACGTTCGGGCGAATTCCGGTTTCGATTCCGGTTTATTACTCTCCCTGTTACGTTACATATTACTAG

mRNA sequence

ATGAGGAAGAACACGATGAGCAGAGAGAATCGATGGGGTCCGAGAAGACGAAAACCAATTATTCGAGAGGTCTGGGCTTGGAATTTCGAGTCTGAAATCGCCCGCTTCGATCGCTGCCTTGAATCGTACGCGATTCTGTCGATCGACACCGAGTTTCCTGGGTGTATTCAGACTGCTCCTTGGGGCGCCATCGACGAACAAGTTTATCAGAATATGAGGGTCAATGTAAATCAGACGAAGCTGATTCAACTGGGTCTGACTGCCTCCGATGAATGCGGGAAGATCGGCGGCGCCTGGGAGTTTAATTTCTGTGATTTCGACGCCGAAACCGACGCCCACAACCCTTCGTCCATTTCGTTCCTGAAACAGAACGGCCTGGATTTCGACCGATTCAAATCCGACGGGATTCCACTGGTTGCATTCACGGCGATTTTCGTTCAAATCTTACGAAAGAACCAAATCTTCCGATGGGTCACATTTCACGGCCTGTACGATATCGGATACCTCTTGAAGGCGATGGGGGTGGTCGAGGAGTTGCCGGAATCAATTGAGGATTTCGGGAGTTTAGTGGTGAATGCGGTAGGCAGTGTGCGCGATCTGAAGCACATTGCTCGGTTCTGTGAAGGGCTGGAAGATGGGAAATTCGGCCTGGAAAGACTGGGCGAACTGCTCGAGCAAAAGCGGTTCGGATCGAAGCACCATGCCGGCTCAGACAGTCTGCTGACGGCTGCACTGCACACGAGGATGATGGAACGGTTTGAACCGGATTCGGAAATCTGCGACGGGTTTCTGTATGGGCTTACAAACAAACTCCAAAACATGGAGATGATGAGGATGAAGGCGGTTCAGACCATAACGTTCGGGCGAATTCCGGTTTCGATTCCGGTTTATTACTCTCCCTGTTACGTTACATATTACTAG

Coding sequence (CDS)

ATGAGGAAGAACACGATGAGCAGAGAGAATCGATGGGGTCCGAGAAGACGAAAACCAATTATTCGAGAGGTCTGGGCTTGGAATTTCGAGTCTGAAATCGCCCGCTTCGATCGCTGCCTTGAATCGTACGCGATTCTGTCGATCGACACCGAGTTTCCTGGGTGTATTCAGACTGCTCCTTGGGGCGCCATCGACGAACAAGTTTATCAGAATATGAGGGTCAATGTAAATCAGACGAAGCTGATTCAACTGGGTCTGACTGCCTCCGATGAATGCGGGAAGATCGGCGGCGCCTGGGAGTTTAATTTCTGTGATTTCGACGCCGAAACCGACGCCCACAACCCTTCGTCCATTTCGTTCCTGAAACAGAACGGCCTGGATTTCGACCGATTCAAATCCGACGGGATTCCACTGGTTGCATTCACGGCGATTTTCGTTCAAATCTTACGAAAGAACCAAATCTTCCGATGGGTCACATTTCACGGCCTGTACGATATCGGATACCTCTTGAAGGCGATGGGGGTGGTCGAGGAGTTGCCGGAATCAATTGAGGATTTCGGGAGTTTAGTGGTGAATGCGGTAGGCAGTGTGCGCGATCTGAAGCACATTGCTCGGTTCTGTGAAGGGCTGGAAGATGGGAAATTCGGCCTGGAAAGACTGGGCGAACTGCTCGAGCAAAAGCGGTTCGGATCGAAGCACCATGCCGGCTCAGACAGTCTGCTGACGGCTGCACTGCACACGAGGATGATGGAACGGTTTGAACCGGATTCGGAAATCTGCGACGGGTTTCTGTATGGGCTTACAAACAAACTCCAAAACATGGAGATGATGAGGATGAAGGCGGTTCAGACCATAACGTTCGGGCGAATTCCGGTTTCGATTCCGGTTTATTACTCTCCCTGTTACGTTACATATTACTAG

Protein sequence

MRKNTMSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERFEPDSEICDGFLYGLTNKLQNMEMMRMKAVQTITFGRIPVSIPVYYSPCYVTYY
Homology
BLAST of Lag0009306 vs. NCBI nr
Match: XP_038895744.1 (putative CCR4-associated factor 1 homolog 8 [Benincasa hispida])

HSP 1 Score: 406.8 bits (1044), Expect = 1.7e-109
Identity = 190/294 (64.63%), Postives = 243/294 (82.65%), Query Frame = 0

Query: 15  RRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRV 74
           R RK +I +VWA NF++EI++ + CLE + I+SIDTEFPG I+ +PWG+IDE +Y++ R 
Sbjct: 5   RIRKSLIHKVWASNFDAEISKLEECLEFHTIVSIDTEFPGFIRNSPWGSIDETLYEDFRF 64

Query: 75  NVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSD 134
           NVNQTKLIQLGLTA D+ GKIGG+WEFNF DFD E DA +PSS SFL+QNGLDF++ K  
Sbjct: 65  NVNQTKLIQLGLTACDDSGKIGGSWEFNFSDFDPEIDAQSPSSASFLEQNGLDFNKLKKY 124

Query: 135 GIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAV 194
           GIP+  FTA FV I+RK+ +FRWVTFHGLYDIGYL+KAMG+ E LPE++E+F ++VV  +
Sbjct: 125 GIPINLFTAKFVHIIRKHNVFRWVTFHGLYDIGYLIKAMGITEVLPETMEEFATVVVRRL 184

Query: 195 GSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERFE 254
           G VRDLKH+ARFCEGLEDGK GLERLG+LL+QKR+G KH+AGSDSLLTA+ H  M++RF+
Sbjct: 185 GIVRDLKHMARFCEGLEDGKLGLERLGKLLDQKRYGLKHNAGSDSLLTASAHFEMIQRFD 244

Query: 255 PDSEICDGFLYGLTNKLQNMEM-MRMKAVQTITFGRIPVSIPVY-YSPCYVTYY 307
            D+EICDG+LYG +NKLQ+M++ M M   + ITFG+IP +IP+Y Y P Y+TYY
Sbjct: 245 MDAEICDGYLYGFSNKLQSMDINMNMFHRKIITFGQIPYAIPLYCYVPSYITYY 298

BLAST of Lag0009306 vs. NCBI nr
Match: KAG6604931.1 (putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027010.1 putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.5 bits (981), Expect = 3.4e-102
Identity = 184/289 (63.67%), Postives = 224/289 (77.51%), Query Frame = 0

Query: 6   MSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAID 65
           M R NR  PRRR P I+EVWA NF+ EI R +RCL++  ILSIDTEFPGCI++ PWG+ID
Sbjct: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 66  EQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 125
           E+VY+++R NVNQTKLIQLGLTASDE G++ G+WEFNF DFD + D  NPSSISFL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120

Query: 126 LDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIED 185
           LDF + K  GIPL  FT  F +I+R++++ +WVTFHGLYDIGYL+KAMGV EELPES+E+
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 186 FGSLVVNAVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAAL 245
           F  +V   VG VRDLK +AR+CEGLE G+ GLERLGELL +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 246 HTRMMERFEPDSEICDGFLYGLTNKLQNMEMMRMKAVQTITFGRIPVSI 295
           H +M   F  +S   DG LYGL NKL++     MK  QTI FG+I  S+
Sbjct: 241 HAKMARWFRLESGAYDGHLYGLQNKLEH-----MKVNQTIMFGQISCSL 284

BLAST of Lag0009306 vs. NCBI nr
Match: XP_022146113.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 382.1 bits (980), Expect = 4.5e-102
Identity = 182/301 (60.47%), Postives = 224/301 (74.42%), Query Frame = 0

Query: 6   MSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAID 65
           M R  R   RR    IREVW+ NF+ E+A  + CL  Y IL+IDTEFPG I++ PWGAI+
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 66  EQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 125
           E++Y + R NVN+TKLIQLGLTASDE G IG  WEFNF DFD + D HN SS+SFLKQNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 126 LDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIED 185
           LDFD+ K  GIP+  FT+    + RK++I RWVTFHGLYDIGYLLKAMGV  E+PES+E+
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 186 FGSLVVNAVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAAL 245
           F  LV  A+G+V DLKH+A+FCEGLEDGK GLE+L +L+ QKRFG+KHHAGSDSLLTA+L
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 246 HTRMMERFEPDSEICDGFLYGLTNKLQNMEMMRMKAVQTITFGRIPVSIPVYYSPCYVTY 305
           + +M  +FE  SE+CDGFLYGL  KL      ++K  Q + FG+IP  +   Y PCY+ Y
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLH----AKIKLYQNLMFGQIPYGLRACYPPCYIAY 297

Query: 306 Y 307
           Y
Sbjct: 301 Y 297

BLAST of Lag0009306 vs. NCBI nr
Match: KAG6604930.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.9 bits (969), Expect = 8.5e-101
Identity = 169/262 (64.50%), Postives = 209/262 (79.77%), Query Frame = 0

Query: 13  GPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNM 72
           GPR   P+IREVWA+N + EI R DRCL SY+++SIDTEFPGCI+  PWG IDE++Y + 
Sbjct: 7   GPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADF 66

Query: 73  RVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFK 132
           R NVNQTK+IQLG+T SDE G IGG WEFNF DFD E DAHNP+SI FLKQNGLDF + K
Sbjct: 67  RFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLK 126

Query: 133 SDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVN 192
            DGI +  F   F+  LRK+ I+RW+TFHGLYDIGYL+ AMGVV+ LPE++ +F  +V  
Sbjct: 127 KDGIKVRKFAIRFIYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVAR 186

Query: 193 AVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMER 252
            VG+VRDLKH+ARFCEGLE G  GLE+LG+LL+QKRFG KHHAGSDSL+TA LH +M++R
Sbjct: 187 RVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQR 246

Query: 253 FEPDSEICDGFLYGLTNKLQNM 275
           ++  +EICDGFLYGL+ K + +
Sbjct: 247 YDFHAEICDGFLYGLSKKFEEL 268

BLAST of Lag0009306 vs. NCBI nr
Match: XP_022947867.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-100
Identity = 177/269 (65.80%), Postives = 214/269 (79.55%), Query Frame = 0

Query: 6   MSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAID 65
           M R NR  PRRR P I+EVWA NF+ EI R +RCL++  ILSIDTEFPGCI++ PWG+ID
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 66  EQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 125
           E+VY+++R NVNQTKLIQLGLTASDE G++ G+WEFNF DFD + D  NPSSI+FL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 126 LDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIED 185
           LDF + K  GIPL  FT  F +I+R++++ +WVTFHGLYDIGYL+KAMGV EELPES+E+
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 186 FGSLVVNAVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAAL 245
           F  +V   VG VRDLK +AR+CEGLE G+ GLERLGELL +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 246 HTRMMERFEPDSEICDGFLYGLTNKLQNM 275
           H +M   F  +SE  DG LYGL NKLQ M
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKLQYM 269

BLAST of Lag0009306 vs. ExPASy Swiss-Prot
Match: Q9C6M9 (Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CAF1-4 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 107/276 (38.77%), Postives = 153/276 (55.43%), Query Frame = 0

Query: 23  EVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLI 82
           EVW WN E E+     CL+ ++ ++IDTEFPGC++  P  A +E  Y++M+ NV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 83  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFT 142
           QLG T  D  G I   WE N  DF+      N  SI+FLK NGL+ D+   +GI +  F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 143 AIFVQILR-KNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGS-VRDL 202
             F QIL+ K+    WV F G YD  YL+K +   + LPE+ E+F   V   +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 203 KHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERFEPDSEIC 262
           K IA  C GL   +FGL+R+ ++L+ KR G  HHAGSDS LTA + T++        + C
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKLTFDLLNSRKQC 242

Query: 263 DGFLYGLTNKLQNMEMMRMKAVQTITFGRIPVSIPV 297
              +     +L+  ++M  +         IP+ IP+
Sbjct: 243 VRRVDHQQYQLEQQQLMMTRCY-------IPIPIPI 269

BLAST of Lag0009306 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 106/262 (40.46%), Postives = 148/262 (56.49%), Query Frame = 0

Query: 23  EVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLI 82
           EVW WN ++E+     CL+    ++IDTEFPGC++  P  A DE  Y++M+ NV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 83  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFT 142
           QLGLT   +   I   WE N  DF+      N  SI+FLK NGLD D+ + +GI +  F 
Sbjct: 64  QLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 143 AIFVQIL-RKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGS-VRDL 202
             F QIL  K+   RWVTF G YD  YLLK +   + LPE+ ++F   V   +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 203 KHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMM-----ERFEP 262
           K +A  C GL   +FGL+R+ ++L+ +R G  HHAGSDS LTA + T+++      R E 
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKLIFDLVNSRKES 243

Query: 263 DSEICDGFLYGLTNKLQNMEMM 278
                D   Y L  +    ++M
Sbjct: 244 TGRRADDQQYQLEQQQHQQQLM 261

BLAST of Lag0009306 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.7e-46
Identity = 95/252 (37.70%), Postives = 144/252 (57.14%), Query Frame = 0

Query: 18  KPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVN 77
           +P+ R VW  N + E+AR   CL+ + +++ DTE+PG I    + +  ++ Y+ M+ NV 
Sbjct: 6   QPLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVE 65

Query: 78  QTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIP 137
            TKLIQ G T  +  G+IGG WE NF +F   +D  N  SI FL+++GLD  + + +G+ 
Sbjct: 66  NTKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVD 125

Query: 138 L--VAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVG 197
           +    F    + + R  +   +VTF G YD  Y L  +    +LPE+  +F + VV   G
Sbjct: 126 MFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILN-HGKLPETHGEFATEVVKVFG 185

Query: 198 SVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERFEP 257
            V D K +A FCEGL +   GL +L +LL+  R G  HHAGSDSL+TA +  ++   +E 
Sbjct: 186 QVYDTKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE- 245

Query: 258 DSEICDGFLYGL 268
           DS    G +YG+
Sbjct: 246 DSRFARGLIYGI 254

BLAST of Lag0009306 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.2e-46
Identity = 100/229 (43.67%), Postives = 135/229 (58.95%), Query Frame = 0

Query: 23  EVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLI 82
           EVW WN E E+     CL+  + ++IDTEFPGC++  P  A +E  Y++M+ NV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 83  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFT 142
           QLG T  D  G     WE N  DFD      N  SI+FLK NGL+ D+ + +GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 143 AIFVQIL-RKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGS-VRDL 202
             F QIL  K+    WV F G YD  YL+K +   + LPE+ E+F   V   +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 203 KHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRM 250
           K IA  C GL   +FGL+R+ ++L+ KR G  HHAGSDS LTA + T++
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

BLAST of Lag0009306 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 3.8e-43
Identity = 95/216 (43.98%), Postives = 128/216 (59.26%), Query Frame = 0

Query: 39  CLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLIQLGLTASDECGKIGGA 98
           CL SY  ++IDTEFP  ++     A DE+ Y +M  +V++ KLIQLGLT  D  G+IGG 
Sbjct: 7   CLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRIGGT 66

Query: 99  WEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVA-FTAIFVQILRKNQIFRW 158
           WE NF DF  + DA N  SI FL++NGLD  + + +GI +   F+ +F  + +  +   W
Sbjct: 67  WEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRNITW 126

Query: 159 VTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGSVRDLKHIARFCEGLEDGKFGL 218
           VTFHG YDI YLLK     E LP + E F   V   +GSV DLK +A  CEGL   + GL
Sbjct: 127 VTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSRLGL 186

Query: 219 ERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERF 254
           E L       R G+ HHAGS++ LTA +  +++  F
Sbjct: 187 ETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

BLAST of Lag0009306 vs. ExPASy TrEMBL
Match: A0A6J1CXP9 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 2.2e-102
Identity = 182/301 (60.47%), Postives = 224/301 (74.42%), Query Frame = 0

Query: 6   MSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAID 65
           M R  R   RR    IREVW+ NF+ E+A  + CL  Y IL+IDTEFPG I++ PWGAI+
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 66  EQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 125
           E++Y + R NVN+TKLIQLGLTASDE G IG  WEFNF DFD + D HN SS+SFLKQNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 126 LDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIED 185
           LDFD+ K  GIP+  FT+    + RK++I RWVTFHGLYDIGYLLKAMGV  E+PES+E+
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 186 FGSLVVNAVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAAL 245
           F  LV  A+G+V DLKH+A+FCEGLEDGK GLE+L +L+ QKRFG+KHHAGSDSLLTA+L
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 246 HTRMMERFEPDSEICDGFLYGLTNKLQNMEMMRMKAVQTITFGRIPVSIPVYYSPCYVTY 305
           + +M  +FE  SE+CDGFLYGL  KL      ++K  Q + FG+IP  +   Y PCY+ Y
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLH----AKIKLYQNLMFGQIPYGLRACYPPCYIAY 297

Query: 306 Y 307
           Y
Sbjct: 301 Y 297

BLAST of Lag0009306 vs. ExPASy TrEMBL
Match: A0A6J1G7L2 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 5.4e-101
Identity = 177/269 (65.80%), Postives = 214/269 (79.55%), Query Frame = 0

Query: 6   MSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAID 65
           M R NR  PRRR P I+EVWA NF+ EI R +RCL++  ILSIDTEFPGCI++ PWG+ID
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 66  EQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 125
           E+VY+++R NVNQTKLIQLGLTASDE G++ G+WEFNF DFD + D  NPSSI+FL+QNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 126 LDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIED 185
           LDF + K  GIPL  FT  F +I+R++++ +WVTFHGLYDIGYL+KAMGV EELPES+E+
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 186 FGSLVVNAVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAAL 245
           F  +V   VG VRDLK +AR+CEGLE G+ GLERLGELL +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 246 HTRMMERFEPDSEICDGFLYGLTNKLQNM 275
           H +M   F  +SE  DG LYGL NKLQ M
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKLQYM 269

BLAST of Lag0009306 vs. ExPASy TrEMBL
Match: A0A6J1G7T4 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.0e-99
Identity = 169/262 (64.50%), Postives = 207/262 (79.01%), Query Frame = 0

Query: 13  GPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNM 72
           GPR   P+IREVWA+N + EI R DRCL SY+I+SIDTEFPGCI+  PWG  DE++Y + 
Sbjct: 7   GPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDEELYADF 66

Query: 73  RVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFK 132
           R NVNQTK+IQLG+T SDE G IGG WEFNF DFD E DAHNP+SI FLKQNGLDF + K
Sbjct: 67  RFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLK 126

Query: 133 SDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVN 192
            DGI +  F   F+  L K+ I+RWVTFHGLYDIGYL+ AMGVV+ LPE++ +F  +V  
Sbjct: 127 KDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFKWIVAR 186

Query: 193 AVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMER 252
            VG+VRDLKH+ARFCEGLE G  GLE+LG+LL+QKRFG KHHAGSDSL+TA LH +M++R
Sbjct: 187 RVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQR 246

Query: 253 FEPDSEICDGFLYGLTNKLQNM 275
           ++  +EICDGFLYGL+ K + +
Sbjct: 247 YDFHAEICDGFLYGLSKKFEEL 268

BLAST of Lag0009306 vs. ExPASy TrEMBL
Match: A0A0A0LPP0 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G270190 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 9.5e-98
Identity = 178/305 (58.36%), Postives = 229/305 (75.08%), Query Frame = 0

Query: 6   MSRENRWGPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAID 65
           M   N+    R KP+IR+VWA NF+SEIARFD CL  + IL+IDTEFPG I  +P G+ID
Sbjct: 1   MGGRNQTRSTREKPLIRQVWASNFDSEIARFDECLRFHTILTIDTEFPGFIAQSPRGSID 60

Query: 66  EQVYQNMRVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNG 125
           +++Y++   NVNQTKLIQLG+TASD+ G+IGG+WEFNF DFD E DAH+P +I FL+ NG
Sbjct: 61  DELYKDFCFNVNQTKLIQLGITASDDLGQIGGSWEFNFSDFDFEADAHSPYAIPFLEHNG 120

Query: 126 LDFDRFKSDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIED 185
           LD  + K DGIP+ +FT  F+ ILRK  IFRWVTFHGLYDIGYL+KAMG++  LP+S+E+
Sbjct: 121 LDLKKMKKDGIPIASFTKKFLPILRKRDIFRWVTFHGLYDIGYLIKAMGLITVLPKSMEE 180

Query: 186 FGSLVVNAVGSVRDLKHIARFCEGLED-GKFGLERLGELLEQKRFGSKHHAGSDSLLTAA 245
           F ++VVN VG VRDLKH+A+FCEGL+D G+ GLERLG+LL  KRFG KH+AGSDSLLTA+
Sbjct: 181 FATVVVNEVGIVRDLKHMAKFCEGLDDHGRLGLERLGKLLNLKRFGMKHNAGSDSLLTAS 240

Query: 246 LHTRMMERFEPDSEICDGFLYGLTNKLQNMEM-MRMKAV--QTITFGRIPVSIPVYYSPC 305
            H  M+ERF  +S++C+GFLYG +  L++M+M M+MK      + F          Y PC
Sbjct: 241 AHLEMVERFGMNSKVCNGFLYGFSETLESMKMKMKMKICHHNILRFS--------LYIPC 297

Query: 306 YVTYY 307
           Y  +Y
Sbjct: 301 YFVFY 297

BLAST of Lag0009306 vs. ExPASy TrEMBL
Match: A0A6J1I6H7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.8e-96
Identity = 163/266 (61.28%), Postives = 205/266 (77.07%), Query Frame = 0

Query: 13  GPRRRKPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNM 72
           GPR   P+I EVWA+N + EI R D CL  Y ++SIDTEFPGCI+  PWG IDE++Y + 
Sbjct: 7   GPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDEELYADF 66

Query: 73  RVNVNQTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFK 132
           R NVNQTK+IQLG+T SDE G IGG WEFNF DFD E DAHNP+SI FLKQNGLDF + K
Sbjct: 67  RFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLK 126

Query: 133 SDGIPLVAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVN 192
            DGI +  F   F+  +RK+ I +W+TFHGLYDIGYL+ A+GVV+ LPE++ +F  +V  
Sbjct: 127 KDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEFEWIVAR 186

Query: 193 AVGSVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMER 252
            VG+VRDLKH+ARFCEGLE G  GLE+LG+LL+QKRFG KHHAGSDSL+TA LH +M++ 
Sbjct: 187 RVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQL 246

Query: 253 FEPDSEICDGFLYGLTNKLQNMEMMR 279
           ++ ++EICDGFLYGL+ K +    M+
Sbjct: 247 YDFNAEICDGFLYGLSKKFEEFVGMQ 272

BLAST of Lag0009306 vs. TAIR 10
Match: AT1G27890.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 8.9e-48
Identity = 107/276 (38.77%), Postives = 153/276 (55.43%), Query Frame = 0

Query: 23  EVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLI 82
           EVW WN E E+     CL+ ++ ++IDTEFPGC++  P  A +E  Y++M+ NV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 83  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFT 142
           QLG T  D  G I   WE N  DF+      N  SI+FLK NGL+ D+   +GI +  F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 143 AIFVQILR-KNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGS-VRDL 202
             F QIL+ K+    WV F G YD  YL+K +   + LPE+ E+F   V   +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 203 KHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERFEPDSEIC 262
           K IA  C GL   +FGL+R+ ++L+ KR G  HHAGSDS LTA + T++        + C
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKLTFDLLNSRKQC 242

Query: 263 DGFLYGLTNKLQNMEMMRMKAVQTITFGRIPVSIPV 297
              +     +L+  ++M  +         IP+ IP+
Sbjct: 243 VRRVDHQQYQLEQQQLMMTRCY-------IPIPIPI 269

BLAST of Lag0009306 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 8.9e-48
Identity = 106/262 (40.46%), Postives = 148/262 (56.49%), Query Frame = 0

Query: 23  EVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLI 82
           EVW WN ++E+     CL+    ++IDTEFPGC++  P  A DE  Y++M+ NV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 83  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFT 142
           QLGLT   +   I   WE N  DF+      N  SI+FLK NGLD D+ + +GI +  F 
Sbjct: 64  QLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 143 AIFVQIL-RKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGS-VRDL 202
             F QIL  K+   RWVTF G YD  YLLK +   + LPE+ ++F   V   +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 203 KHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMM-----ERFEP 262
           K +A  C GL   +FGL+R+ ++L+ +R G  HHAGSDS LTA + T+++      R E 
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKLIFDLVNSRKES 243

Query: 263 DSEICDGFLYGLTNKLQNMEMM 278
                D   Y L  +    ++M
Sbjct: 244 TGRRADDQQYQLEQQQHQQQLM 261

BLAST of Lag0009306 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.4e-47
Identity = 95/252 (37.70%), Postives = 144/252 (57.14%), Query Frame = 0

Query: 18  KPIIREVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVN 77
           +P+ R VW  N + E+AR   CL+ + +++ DTE+PG I    + +  ++ Y+ M+ NV 
Sbjct: 6   QPLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVE 65

Query: 78  QTKLIQLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIP 137
            TKLIQ G T  +  G+IGG WE NF +F   +D  N  SI FL+++GLD  + + +G+ 
Sbjct: 66  NTKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVD 125

Query: 138 L--VAFTAIFVQILRKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVG 197
           +    F    + + R  +   +VTF G YD  Y L  +    +LPE+  +F + VV   G
Sbjct: 126 MFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILN-HGKLPETHGEFATEVVKVFG 185

Query: 198 SVRDLKHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERFEP 257
            V D K +A FCEGL +   GL +L +LL+  R G  HHAGSDSL+TA +  ++   +E 
Sbjct: 186 QVYDTKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE- 245

Query: 258 DSEICDGFLYGL 268
           DS    G +YG+
Sbjct: 246 DSRFARGLIYGI 254

BLAST of Lag0009306 vs. TAIR 10
Match: AT1G27820.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.4e-47
Identity = 100/229 (43.67%), Postives = 135/229 (58.95%), Query Frame = 0

Query: 23  EVWAWNFESEIARFDRCLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLI 82
           EVW WN E E+     CL+  + ++IDTEFPGC++  P  A +E  Y++M+ NV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 83  QLGLTASDECGKIGGAWEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVAFT 142
           QLG T  D  G     WE N  DFD      N  SI+FLK NGL+ D+ + +GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 143 AIFVQIL-RKNQIFRWVTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGS-VRDL 202
             F QIL  K+    WV F G YD  YL+K +   + LPE+ E+F   V   +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 203 KHIARFCEGLEDGKFGLERLGELLEQKRFGSKHHAGSDSLLTAALHTRM 250
           K IA  C GL   +FGL+R+ ++L+ KR G  HHAGSDS LTA + T++
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

BLAST of Lag0009306 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 176.8 bits (447), Expect = 2.7e-44
Identity = 95/216 (43.98%), Postives = 128/216 (59.26%), Query Frame = 0

Query: 39  CLESYAILSIDTEFPGCIQTAPWGAIDEQVYQNMRVNVNQTKLIQLGLTASDECGKIGGA 98
           CL SY  ++IDTEFP  ++     A DE+ Y +M  +V++ KLIQLGLT  D  G+IGG 
Sbjct: 7   CLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRIGGT 66

Query: 99  WEFNFCDFDAETDAHNPSSISFLKQNGLDFDRFKSDGIPLVA-FTAIFVQILRKNQIFRW 158
           WE NF DF  + DA N  SI FL++NGLD  + + +GI +   F+ +F  + +  +   W
Sbjct: 67  WEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRNITW 126

Query: 159 VTFHGLYDIGYLLKAMGVVEELPESIEDFGSLVVNAVGSVRDLKHIARFCEGLEDGKFGL 218
           VTFHG YDI YLLK     E LP + E F   V   +GSV DLK +A  CEGL   + GL
Sbjct: 127 VTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSRLGL 186

Query: 219 ERLGELLEQKRFGSKHHAGSDSLLTAALHTRMMERF 254
           E L       R G+ HHAGS++ LTA +  +++  F
Sbjct: 187 ETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895744.11.7e-10964.63putative CCR4-associated factor 1 homolog 8 [Benincasa hispida][more]
KAG6604931.13.4e-10263.67putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp.... [more]
XP_022146113.14.5e-10260.47putative CCR4-associated factor 1 homolog 8 [Momordica charantia][more]
KAG6604930.18.5e-10164.50putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022947867.11.1e-10065.80putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9C6M91.2e-4638.77Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
O647731.2e-4640.46Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SHJ04.7e-4637.70Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SFX66.2e-4643.67Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LXM43.8e-4343.98Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1CXP92.2e-10260.47Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=... [more]
A0A6J1G7L25.4e-10165.80Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3... [more]
A0A6J1G7T41.0e-9964.50Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3... [more]
A0A0A0LPP09.5e-9858.36Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G270190 PE=3 SV... [more]
A0A6J1I6H71.8e-9661.28Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G27890.18.9e-4838.77Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G61470.18.9e-4840.46Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G06450.13.4e-4737.70Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G27820.14.4e-4743.67Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G44240.12.7e-4443.98Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 26..138
e-value: 7.5E-11
score: 41.9
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 10..271
e-value: 5.5E-70
score: 237.5
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 19..290
NoneNo IPR availablePANTHERPTHR10797:SF57CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATEDcoord: 19..290
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 15..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009306.1Lag0009306.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006402 mRNA catabolic process
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030014 CCR4-NOT complex
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity