Lag0007535 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0007535
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionaspartic proteinase Asp1-like
Locationchr9: 759777 .. 762851 (-)
RNA-Seq ExpressionLag0007535
SyntenyLag0007535
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAAGGGGTATTGATGATATTGGTGCTGATGGTGTCCTCTATTTCCATGAGCTGTTTGGCTCCATGTTCAGCTTCTTCGTTTTTCAAGGATAAGCCATGGGAGAGAAGGAGGCCAATTCTGTCGGTTCCGACAGCATCCTCTTCGTTTGCTTCGTCCTCCATTGTGTTGCCTCTTCAAGGGAACGTCTTTCCAAATGGGTAAGCCATAACTCTGCTCACACCTCTACGAAGTTTCAGCTTTTGCTTTTCCCATTTCGATTTTACTTTCAACTGTTACAGAGTACGGAAGTGGCTTTATTTAGAGGAAGTTCAATGTGAATGTGATAGCTCTTCAGATACTCTTCACATTGATGTCAATGATCTTAATTCTTAATGTGTTGTAATAATCTCGGAAGAACTGGAATTGGGATTTGGAAGGCCTTTGCCATTTTGATCTTATTGTATCTTCTCCCGCTTGTTAAATTGGAAAAAAGAAGATCACGAGACTCTGTTTTCCCATGTTTACTTTCTGCCCTGTGTTTCTTATCCTATTCCTGTTCAATTGGTTTGGAAGAGTGCTTTTCTGAGGTTTTTCATTTTGGAGACGAAGAACACATTTCCAAATGAAGTGTTTCTAAAATCTTCAAACTCATTTTACGTAATGTTTTCATTAGTTAAAAACACGTTGAAGATATATGTTTCTCTCTATATAATGATTTTCACGAACATGTTTTGAAGTCATATAATCTGTGTCTTTTTTCAGGTTCTATAACGTTACTCTTTATATAGGGCAGCCTCCAAAGCCTTACTTTCTAGATCCAGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAGCAGTGCACTGAGGTAAATTGTTATTTCATTCAGCAGTTAATGCTCATAGGAAGGAGACTCTTGAAAATATGATGACCAAATGAATGGAGAAAGTTACTTGCCTACACCTTTGGCTTCAGTTGAGTTAATTTTCTTCATAAGTAATCCAAATTTTCATCTTTCATTGTCTATTTTGTTTTAAGTTTCGTCTATATTTTTCTTCTTGACGTGCTTTCTCTTTTCGCTTTTAGTCTCTGAGTAATCACCTTTAAAAATCTGTCATTTCTTTTCGAGTTGAGATTTAAAGATCGTATGCATCAAATTGATGGCCTTGTTTCCCTTTCAGACACCTCATCCGCTCTATCAACCGAGCAACGATCTTGTGCCGTGTAAGGACCCTCTTTGTATGTCCTTGCACTCATCTATTGACCACAGATGTGAGAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCGTCTCTTGGAGTCCTTGTCAGGGATATATTTCCTCTCAACTTAACCAATGGAGATCCAATTAGACCTCGTTTGACCCTCGGGTAAACCGCTTGACTGTCTTCAGTAATTTGTCTTAATCTCATTATATTGTTGAGTTTCTGGCAATCTAAATCAACCTTGCTACTTCCAATTGAACCAATCATGAGATCTTGGCAAAAACAAACACCATATCATGCTGTCATATCTTATTTTTTGCACTCTCCTGATGTTGCAAGATAATGAACAGCATGAATGTACAGTTAGAGTTTATGTCGAATTCCAAACGTGTGTATTCGTGCGCTGGGTTACTTCAAGAAGCCACATGATAAAGATGGCAACAGTCACTAGATTTTATGACGGGAATAATGATTCTGATTTGTATCTTCAATAGTCATCAATAAGGATTATACTGTACAAGAGATTTATATTCACTTTGCTAAAAAATTCCATACATTGCTAGTCCTTGCAAACTGTAGTTTTCAGTTCATGTGGGTAAATAAAATGCATGTAAAGTCTGTATGTAAGTCAGTTACTTGAATCTGTAGTACTGAGTTTTGTTTCATTTGATTACTCAGATGTGGTTATGATCAAGATCCTGGATCATCATCTTATCACCCCATGGATGGAATACTTGGCCTTGGAAAGGGAGCAGTAAGCATCGTCTCACAGCTGCATAATCAGGGCATTATCCGAAACGTCGTTGGTCACTGTTTCAGCAGCAAGGGAGGAGGATATCTTTTCTTTGGGGATGATATTTATGATTCCTATCGCTTAGTTTGGACGCCCATGTCTCGGGACTACCCGTAAATAACCTACATCGATCATATATTTATCTTGTTATGTATGATTCATGTTGGCTCCTGTTCTTAGAAAGCCACGCTTCATGTCATTGTATCAGGAAGCATTACTCTCCCGGGTTTGGAGAACTACTCTTCAATGGAAGAAGTACTGGACTCAGAAACCTCTTCGTAGTTTTTGACAGTGGGAGCTCTTACACCTACTTCAATGCTCAGGCTTATCAAGTTTTAACATCTTTGGTAAATCATCTGTAGAAAGAACCTCTGATTCTATTGTTGGGAAAGTTTTTCGATGACTATTTAATTCATCTTGGCACTTTTCTTGTAGTTGAATAGAGAACTAGCTGGGAAACCACTAAGAGAAGCCGAGGACGACGACACGCTTCCGCTCTGCTGGAGAGGACGAAAGCCATTCAAAAGTTTACGTGATGTGAGAAAATATTTCAAGCCGTTAGCTTTGAGCTTTCCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAATGGAAGGTTATCTTATAATATCGGTAAGCTTCAAGCCTCCCAACTCCAACTACGATTCACTACCATTTGCTTTGGATTTTTAAGTAGAACGATTTAACATATAAGTTATGGAATGGCAGTCCATGGGAAACGTTTGCTTGGGAATTCTAAACGGCACTGAAGTTGGGCTTGAAAACTCCAATATCATTGGTGGTACGTACTTTATTTTGCATGCATTGCATGTAACAAATGCCTTCAAAGCCATGCAAAATTATTCTTTTATTAGAGAACAAAAAAAAAAAAAAAAAGAAAAGCCTCATCTTCTGTGGGGTTTTGTTGACAGATATATCGATGCAAGATAAGATGGTAGTATACAACAACGAGAAGCAAGCAATTGGATGGGCTACTGCTAACTGTGATCGGGTTCCCAAGTCTAGAGTCAGTAGCATGTAA

mRNA sequence

ATGGGGAAAGGGGTATTGATGATATTGGTGCTGATGGTGTCCTCTATTTCCATGAGCTGTTTGGCTCCATGTTCAGCTTCTTCGTTTTTCAAGGATAAGCCATGGGAGAGAAGGAGGCCAATTCTGTCGGTTCCGACAGCATCCTCTTCGTTTGCTTCGTCCTCCATTGTGTTGCCTCTTCAAGGGAACGTCTTTCCAAATGGGTTCTATAACGTTACTCTTTATATAGGGCAGCCTCCAAAGCCTTACTTTCTAGATCCAGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAGCAGTGCACTGAGACACCTCATCCGCTCTATCAACCGAGCAACGATCTTGTGCCGTGTAAGGACCCTCTTTGTATGTCCTTGCACTCATCTATTGACCACAGATGTGAGAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCGTCTCTTGGAGTCCTTGTCAGGGATATATTTCCTCTCAACTTAACCAATGGAGATCCAATTAGACCTCGTTTGACCCTCGGATGTGGTTATGATCAAGATCCTGGATCATCATCTTATCACCCCATGGATGGAATACTTGGCCTTGGAAAGGGAGCAGTAAGCATCGTCTCACAGCTGCATAATCAGGGCATTATCCGAAACGTCGTTGGTCACTGTTTCAGCAGCAAGGGAGGAGGATATCTTTTCTTTGGGGATGATATTTATGATTCCTATCGCTTAGTTTGGACGCCCATGTCTCGGGACTACCCGAAGCATTACTCTCCCGGGTTTGGAGAACTACTCTTCAATGGAAGAAGTACTGGACTCAGAAACCTCTTCGTAGTTTTTGACAGTGGGAGCTCTTACACCTACTTCAATGCTCAGGCTTATCAAGTTTTAACATCTTTGTTGAATAGAGAACTAGCTGGGAAACCACTAAGAGAAGCCGAGGACGACGACACGCTTCCGCTCTGCTGGAGAGGACGAAAGCCATTCAAAAGTTTACGTGATGTGAGAAAATATTTCAAGCCGTTAGCTTTGAGCTTTCCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAATGGAAGGTTATCTTATAATATCGTCCATGGGAAACGTTTGCTTGGGAATTCTAAACGGCACTGAAGTTGGGCTTGAAAACTCCAATATCATTGGTGATATATCGATGCAAGATAAGATGGTAGTATACAACAACGAGAAGCAAGCAATTGGATGGGCTACTGCTAACTGTGATCGGGTTCCCAAGTCTAGAGTCAGTAGCATGTAA

Coding sequence (CDS)

ATGGGGAAAGGGGTATTGATGATATTGGTGCTGATGGTGTCCTCTATTTCCATGAGCTGTTTGGCTCCATGTTCAGCTTCTTCGTTTTTCAAGGATAAGCCATGGGAGAGAAGGAGGCCAATTCTGTCGGTTCCGACAGCATCCTCTTCGTTTGCTTCGTCCTCCATTGTGTTGCCTCTTCAAGGGAACGTCTTTCCAAATGGGTTCTATAACGTTACTCTTTATATAGGGCAGCCTCCAAAGCCTTACTTTCTAGATCCAGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAGCAGTGCACTGAGACACCTCATCCGCTCTATCAACCGAGCAACGATCTTGTGCCGTGTAAGGACCCTCTTTGTATGTCCTTGCACTCATCTATTGACCACAGATGTGAGAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCGTCTCTTGGAGTCCTTGTCAGGGATATATTTCCTCTCAACTTAACCAATGGAGATCCAATTAGACCTCGTTTGACCCTCGGATGTGGTTATGATCAAGATCCTGGATCATCATCTTATCACCCCATGGATGGAATACTTGGCCTTGGAAAGGGAGCAGTAAGCATCGTCTCACAGCTGCATAATCAGGGCATTATCCGAAACGTCGTTGGTCACTGTTTCAGCAGCAAGGGAGGAGGATATCTTTTCTTTGGGGATGATATTTATGATTCCTATCGCTTAGTTTGGACGCCCATGTCTCGGGACTACCCGAAGCATTACTCTCCCGGGTTTGGAGAACTACTCTTCAATGGAAGAAGTACTGGACTCAGAAACCTCTTCGTAGTTTTTGACAGTGGGAGCTCTTACACCTACTTCAATGCTCAGGCTTATCAAGTTTTAACATCTTTGTTGAATAGAGAACTAGCTGGGAAACCACTAAGAGAAGCCGAGGACGACGACACGCTTCCGCTCTGCTGGAGAGGACGAAAGCCATTCAAAAGTTTACGTGATGTGAGAAAATATTTCAAGCCGTTAGCTTTGAGCTTTCCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAATGGAAGGTTATCTTATAATATCGTCCATGGGAAACGTTTGCTTGGGAATTCTAAACGGCACTGAAGTTGGGCTTGAAAACTCCAATATCATTGGTGATATATCGATGCAAGATAAGATGGTAGTATACAACAACGAGAAGCAAGCAATTGGATGGGCTACTGCTAACTGTGATCGGGTTCCCAAGTCTAGAGTCAGTAGCATGTAA

Protein sequence

MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLTLGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDRVPKSRVSSM
Homology
BLAST of Lag0007535 vs. NCBI nr
Match: XP_038900559.1 (aspartic proteinase Asp1 isoform X2 [Benincasa hispida])

HSP 1 Score: 852.0 bits (2200), Expect = 2.2e-243
Identity = 405/431 (93.97%), Postives = 418/431 (96.98%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           M K VLMILVLMV+  SMSCLAPCSASSFFKDKPWERRRPILSVP ASSSFASSSIV+PL
Sbjct: 1   MEKRVLMILVLMVA--SMSCLAPCSASSFFKDKPWERRRPILSVPIASSSFASSSIVMPL 60

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGD 240
           LGCGYDQDPGSSSYHPMDG+LGLG+GAVS+VSQLHNQGI+RNVVGHCFSSKGGGYLFFGD
Sbjct: 181 LGCGYDQDPGSSSYHPMDGVLGLGRGAVSMVSQLHNQGIVRNVVGHCFSSKGGGYLFFGD 240

Query: 241 DIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYD YR+VWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV
Sbjct: 241 GIYDPYRIVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300

Query: 301 LTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVF 360
           LTSLLNRELAGKPLREA DDDTLPLCWRGRKPFKSLRDVRKYFKPLALSF SGGRSKAVF
Sbjct: 301 LTSLLNRELAGKPLREAMDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFSSGGRSKAVF 360

Query: 361 EIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           EIPMEGYLIISSMGN CLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 361 EIPMEGYLIISSMGNACLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420

Query: 421 DRVPKSRVSSM 432
           DRVPKSRV SM
Sbjct: 421 DRVPKSRVGSM 429

BLAST of Lag0007535 vs. NCBI nr
Match: XP_004147327.2 (aspartic proteinase Asp1 isoform X1 [Cucumis sativus] >KAE8651999.1 hypothetical protein Csa_016941 [Cucumis sativus])

HSP 1 Score: 850.5 bits (2196), Expect = 6.3e-243
Identity = 404/430 (93.95%), Postives = 419/430 (97.44%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++LVLMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 1   MGKRVLVVLVLMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 60

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGD 240
           LGCGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYLFFGD
Sbjct: 181 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 240

Query: 241 DIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV
Sbjct: 241 GIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300

Query: 301 LTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVF 360
           LTSLLNRELAGKPLREA DDDTLPLCWRGRKP KSLRDVRKYFKPLALSF SGGRSKAVF
Sbjct: 301 LTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 360

Query: 361 EIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           EIP EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 361 EIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420

Query: 421 DRVPKSRVSS 431
           DRVPKS+VSS
Sbjct: 421 DRVPKSQVSS 428

BLAST of Lag0007535 vs. NCBI nr
Match: XP_008460823.1 (PREDICTED: aspartic proteinase Asp1 isoform X2 [Cucumis melo])

HSP 1 Score: 846.7 bits (2186), Expect = 9.1e-242
Identity = 402/430 (93.49%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++L LMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 1   MGKWVLVVLALMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 60

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGD 240
           LGCGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYLFFGD
Sbjct: 181 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 240

Query: 241 DIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV
Sbjct: 241 GIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300

Query: 301 LTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVF 360
           LTSLLNRELAGKPLREA DDDTLPLCWR RKP KSLRDVRKYFKPLALSF SGGRSKAVF
Sbjct: 301 LTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 360

Query: 361 EIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           EIP+EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 361 EIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420

Query: 421 DRVPKSRVSS 431
           DRVPKS+VSS
Sbjct: 421 DRVPKSQVSS 428

BLAST of Lag0007535 vs. NCBI nr
Match: TYK02025.1 (aspartic proteinase Asp1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 846.7 bits (2186), Expect = 9.1e-242
Identity = 402/430 (93.49%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++L LMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 42  MGKWVLVVLALMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 101

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 102 QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 161

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 162 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 221

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGD 240
           LGCGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYLFFGD
Sbjct: 222 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 281

Query: 241 DIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV
Sbjct: 282 GIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 341

Query: 301 LTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVF 360
           LTSLLNRELAGKPLREA DDDTLPLCWR RKP KSLRDVRKYFKPLALSF SGGRSKAVF
Sbjct: 342 LTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 401

Query: 361 EIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           EIP+EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 402 EIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 461

Query: 421 DRVPKSRVSS 431
           DRVPKS+VSS
Sbjct: 462 DRVPKSQVSS 469

BLAST of Lag0007535 vs. NCBI nr
Match: XP_008460822.1 (PREDICTED: aspartic proteinase Asp1 isoform X1 [Cucumis melo])

HSP 1 Score: 840.9 bits (2171), Expect = 5.0e-240
Identity = 402/434 (92.63%), Postives = 418/434 (96.31%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++L LMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 1   MGKWVLVVLALMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 60

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180

Query: 181 LG----CGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYL 240
           LG    CGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYL
Sbjct: 181 LGCQLICGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYL 240

Query: 241 FFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQ 300
           FFGD IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQ
Sbjct: 241 FFGDGIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQ 300

Query: 301 AYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRS 360
           AYQVLTSLLNRELAGKPLREA DDDTLPLCWR RKP KSLRDVRKYFKPLALSF SGGRS
Sbjct: 301 AYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRS 360

Query: 361 KAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWA 420
           KAVFEIP+EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWA
Sbjct: 361 KAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWA 420

Query: 421 TANCDRVPKSRVSS 431
           TANCDRVPKS+VSS
Sbjct: 421 TANCDRVPKSQVSS 432

BLAST of Lag0007535 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 5.6e-93
Identity = 185/388 (47.68%), Postives = 255/388 (65.72%), Query Frame = 0

Query: 53  SSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPL 112
           SS++VL L GNV+P G + +T+ IG P K YFLD DTGS LTWLQCDAPC  C   PH L
Sbjct: 21  SSAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVL 80

Query: 113 YQPS-NDLVPCKDPLCMSLHSSI--DHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNL 172
           Y+P+   LV C D LC  L++ +    RC +  QCDY ++Y D  SS+GVLV D F L+ 
Sbjct: 81  YKPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSA 140

Query: 173 TNGDPIRPRLTLGCGYDQDPGSSSYH-PMDGILGLGKGAVSIVSQLHNQGII-RNVVGHC 232
           +NG      +  GCGYDQ   + +   P+D ILGL +G V+++SQL +QG+I ++V+GHC
Sbjct: 141 SNGTN-PTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 233 FSSKGGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLF--NGRSTGLRNLFVVFD 292
            SSKGGG+LFFGD    +  + WTPM+R++ K+YSPG G L F  N ++     + V+FD
Sbjct: 201 ISSKGGGFLFFGDAQVPTSGVTWTPMNREH-KYYSPGHGTLHFDSNSKAISAAPMAVIFD 260

Query: 293 SGSSYTYFNAQAYQ----VLTSLLNRELAGKPLRE-AEDDDTLPLCWRGRKPFKSLRDVR 352
           SG++YTYF AQ YQ    V+ S LN E   K L E  E D  L +CW+G+    ++ +V+
Sbjct: 261 SGATYTYFAAQPYQATLSVVKSTLNSEC--KFLTEVTEKDRALTVCWKGKDKIVTIDEVK 320

Query: 353 KYFKPLALSFPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTE--VGLENSNIIGDIS 412
           K F+ L+L F  G + KA  EIP E YLIIS  G+VCLGIL+G++  + L  +N+IG I+
Sbjct: 321 KCFRSLSLEFADGDK-KATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGIT 380

Query: 413 MQDKMVVYNNEKQAIGWATANCDRVPKS 427
           M D+MV+Y++E+  +GW    CDR+P+S
Sbjct: 381 MLDQMVIYDSERSLLGWVNYQCDRIPRS 402

BLAST of Lag0007535 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 327.4 bits (838), Expect = 2.4e-88
Identity = 175/386 (45.34%), Postives = 249/386 (64.51%), Query Frame = 0

Query: 53  SSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPL 112
           SS++VL L GNV+P G + VT+ IG P KPYFLD DTGS LTWLQCD PC  C + PH L
Sbjct: 21  SSAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGL 80

Query: 113 YQPS-NDLVPCKDPLCMSLHSSI--DHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNL 172
           Y+P     V C +  C  L++ +    +C   +QC Y ++Y  GGSS+GVL+ D F L  
Sbjct: 81  YKPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYV-GGSSIGVLIVDSFSLPA 140

Query: 173 TNGDPIRPRLTLGCGYDQDPGSSSY-HPMDGILGLGKGAVSIVSQLHNQGII-RNVVGHC 232
           +NG      +  GCGY+Q   + +   P++GILGLG+G V+++SQL +QG+I ++V+GHC
Sbjct: 141 SNGTN-PTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 233 FSSKGGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGL--RNLFVVFD 292
            SSKG G+LFFGD    +  + W+PM+R++ KHYSP  G L FN  S  +    + V+FD
Sbjct: 201 ISSKGKGFLFFGDAKVPTSGVTWSPMNREH-KHYSPRQGTLQFNSNSKPISAAPMEVIFD 260

Query: 293 SGSSYTYFNAQAYQVLTSLLNRELAG--KPLREA-EDDDTLPLCWRGRKPFKSLRDVRKY 352
           SG++YTYF  Q Y    S++   L+   K L E  E D  L +CW+G+   +++ +V+K 
Sbjct: 261 SGATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKC 320

Query: 353 FKPLALSFPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTE--VGLENSNIIGDISMQ 412
           F+ L+L F  G + KA  EIP E YLIIS  G+VCLGIL+G++    L  +N+IG I+M 
Sbjct: 321 FRSLSLKFADGDK-KATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITML 380

Query: 413 DKMVVYNNEKQAIGWATANCDRVPKS 427
           D+MV+Y++E+  +GW    CDR+P+S
Sbjct: 381 DQMVIYDSERSLLGWVNYQCDRIPRS 402

BLAST of Lag0007535 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 7.6e-82
Identity = 172/397 (43.32%), Postives = 242/397 (60.96%), Query Frame = 0

Query: 46  TASSSFASSSIVLPLQGNVFPNGFYNVTLYIGQPP--KPYFLDPDTGSDLTWLQCDAPCQ 105
           T++ S  SS+ + P+ GNV+P+G Y   + +G+P   + Y LD DTGS+LTW+QCDAPC 
Sbjct: 179 TSAGSIDSSTTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCT 238

Query: 106 QCTETPHPLYQPSND-LVPCKDPLCMSL-HSSIDHRCENPDQCDYEVEYADGGSSLGVLV 165
            C +  + LY+P  D LV   +  C+ +  + +   CEN  QCDYE+EYAD   S+GVL 
Sbjct: 239 SCAKGANQLYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCHQCDYEIEYADHSYSMGVLT 298

Query: 166 RDIFPLNLTNGDPIRPRLTLGCGYDQDP-GSSSYHPMDGILGLGKGAVSIVSQLHNQGII 225
           +D F L L NG      +  GCGYDQ     ++    DGILGL +  +S+ SQL ++GII
Sbjct: 299 KDKFHLKLHNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGII 358

Query: 226 RNVVGHCFSS--KGGGYLFFGDDIYDSYRLVWTPMSRD--------YPKHYSPGFGELLF 285
            NVVGHC +S   G GY+F G D+  S+ + W PM  D             S G G L  
Sbjct: 359 SNVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSL 418

Query: 286 NGRSTGLRNLFVVFDSGSSYTYFNAQAY-QVLTSLLNRELAGKPLREAEDDDTLPLCWRG 345
           +G +  +    V+FD+GSSYTYF  QAY Q++TSL  +E++G  L   + D+TLP+CWR 
Sbjct: 419 DGENGRVGK--VLFDTGSSYTYFPNQAYSQLVTSL--QEVSGLELTRDDSDETLPICWRA 478

Query: 346 RK--PFKSLRDVRKYFKPLALSFPSGGR--SKAVFEIPMEGYLIISSMGNVCLGILNGTE 405
           +   PF SL DV+K+F+P+ L   S     S+ +  I  E YLIIS+ GNVCLGIL+G+ 
Sbjct: 479 KTNFPFSSLSDVKKFFRPITLQIGSKWLIISRKLL-IQPEDYLIISNKGNVCLGILDGSS 538

Query: 406 VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 423
           V   ++ I+GDISM+  ++VY+N K+ IGW  ++C R
Sbjct: 539 VHDGSTIILGDISMRGHLIVYDNVKRRIGWMKSDCVR 570

BLAST of Lag0007535 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 2.2e-33
Identity = 115/391 (29.41%), Postives = 174/391 (44.50%), Query Frame = 0

Query: 54  SSIVLPLQGN--VFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHP 113
           +SI LPL G+  V   G Y   + +G PPK Y +  DTGSD+ W+ C  PC +C    + 
Sbjct: 56  ASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPTKTNL 115

Query: 114 LYQPS---------NDLVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVR 173
            ++ S         +  V C D  C  +  S    C+    C Y + YAD  +S G  +R
Sbjct: 116 NFRLSLFDMNASSTSKKVGCDDDFCSFISQS--DSCQPALGCSYHIVYADESTSDGKFIR 175

Query: 174 DIFPLNLTNGD----PIRPRLTLGCGYDQDPG-SSSYHPMDGILGLGKGAVSIVSQLHNQ 233
           D+  L    GD    P+   +  GCG DQ     +    +DG++G G+   S++SQL   
Sbjct: 176 DMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAAT 235

Query: 234 GIIRNVVGHCFSS-KGGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTG 293
           G  + V  HC  + KGGG   F   + DS ++  TPM  +   HY+     +  +G S  
Sbjct: 236 GDAKRVFSHCLDNVKGGG--IFAVGVVDSPKVKTTPMVPN-QMHYNVMLMGMDVDGTSLD 295

Query: 294 L-----RNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRK 353
           L     RN   + DSG++  YF    Y    SL+   LA +P++    ++T        +
Sbjct: 296 LPRSIVRNGGTIVDSGTTLAYFPKVLYD---SLIETILARQPVKLHIVEETF-------Q 355

Query: 354 PFKSLRDVRKYFKPLALSFPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENS 413
            F    +V + F P++  F           +    YL        C G   G     E S
Sbjct: 356 CFSFSTNVDEAFPPVSFEF----EDSVKLTVYPHDYLFTLEEELYCFGWQAGGLTTDERS 415

Query: 414 NII--GDISMQDKMVVYNNEKQAIGWATANC 421
            +I  GD+ + +K+VVY+ + + IGWA  NC
Sbjct: 416 EVILLGDLVLSNKLVVYDLDNEVIGWADHNC 426

BLAST of Lag0007535 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.1e-27
Identity = 111/373 (29.76%), Postives = 162/373 (43.43%), Query Frame = 0

Query: 69  FYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCT-ETPHP--------LYQP---- 128
           + NVT  +G P   + +  DTGSDL WL CD  C  C  E   P        +Y P    
Sbjct: 105 YANVT--VGTPSDWFMVALDTGSDLFWLPCD--CTNCVRELKAPGGSSLDLNIYSPNASS 164

Query: 129 SNDLVPCKDPLCMSLHSSIDHRCENPD-QCDYEVEY-ADGGSSLGVLVRDIFPL--NLTN 188
           ++  VPC   LC     +   RC +P+  C Y++ Y ++G SS GVLV D+  L  N  +
Sbjct: 165 TSTKVPCNSTLC-----TRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSNDKS 224

Query: 189 GDPIRPRLTLGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSK 248
              I  R+T GCG  Q          +G+ GLG   +S+ S L  +GI  N    CF + 
Sbjct: 225 SKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFGND 284

Query: 249 GGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYT 308
           G G + FGD    S     TP++   P          +  G +TG      VFDSG+S+T
Sbjct: 285 GAGRISFGDK--GSVDQRETPLNIRQPHPTYNITVTKISVGGNTGDLEFDAVFDSGTSFT 344

Query: 309 YFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFP 368
           Y    AY +++   N     K  +  + +     C+         +D  +Y  P      
Sbjct: 345 YLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCY----ALSPNKDSFQY--PAVNLTM 404

Query: 369 SGGRSKAVFE----IPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYN 421
            GG S  V+     IPM+   +       CL I+      +E+ +IIG   M    VV++
Sbjct: 405 KGGSSYPVYHPLVVIPMKDTDV------YCLAIMK-----IEDISIIGQNFMTGYRVVFD 449

BLAST of Lag0007535 vs. ExPASy TrEMBL
Match: A0A5D3BS69 (Aspartic proteinase Asp1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00070 PE=4 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.4e-242
Identity = 402/430 (93.49%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++L LMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 42  MGKWVLVVLALMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 101

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 102 QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 161

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 162 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 221

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGD 240
           LGCGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYLFFGD
Sbjct: 222 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 281

Query: 241 DIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV
Sbjct: 282 GIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 341

Query: 301 LTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVF 360
           LTSLLNRELAGKPLREA DDDTLPLCWR RKP KSLRDVRKYFKPLALSF SGGRSKAVF
Sbjct: 342 LTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 401

Query: 361 EIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           EIP+EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 402 EIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 461

Query: 421 DRVPKSRVSS 431
           DRVPKS+VSS
Sbjct: 462 DRVPKSQVSS 469

BLAST of Lag0007535 vs. ExPASy TrEMBL
Match: A0A1S3CDB2 (aspartic proteinase Asp1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.4e-242
Identity = 402/430 (93.49%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++L LMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 1   MGKWVLVVLALMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 60

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFFGD 240
           LGCGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYLFFGD
Sbjct: 181 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 240

Query: 241 DIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV
Sbjct: 241 GIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300

Query: 301 LTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKAVF 360
           LTSLLNRELAGKPLREA DDDTLPLCWR RKP KSLRDVRKYFKPLALSF SGGRSKAVF
Sbjct: 301 LTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 360

Query: 361 EIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           EIP+EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 361 EIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420

Query: 421 DRVPKSRVSS 431
           DRVPKS+VSS
Sbjct: 421 DRVPKSQVSS 428

BLAST of Lag0007535 vs. ExPASy TrEMBL
Match: A0A1S3CDB4 (aspartic proteinase Asp1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.4e-240
Identity = 402/434 (92.63%), Postives = 418/434 (96.31%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVPTASSSFASSSIVLPL 60
           MGK VL++L LMV+  SMSCLAPCSASSFFKDKPWER+RPILSVPTASSSFASSSIVLPL
Sbjct: 1   MGKWVLVVLALMVA--SMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPL 60

Query: 61  QGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120
           QGNV+PNGFYNVTLY+GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180

Query: 181 LG----CGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYL 240
           LG    CGYDQDPGSSSYHPMDGILGLG+GAVSIVSQLHNQGI+RNVVGHCF+SKGGGYL
Sbjct: 181 LGCQLICGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYL 240

Query: 241 FFGDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQ 300
           FFGD IYD YRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQ
Sbjct: 241 FFGDGIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQ 300

Query: 301 AYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRS 360
           AYQVLTSLLNRELAGKPLREA DDDTLPLCWR RKP KSLRDVRKYFKPLALSF SGGRS
Sbjct: 301 AYQVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRS 360

Query: 361 KAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWA 420
           KAVFEIP+EGY+IISSMGNVCLGILNGT+VGLENSNIIGDISMQDKMVVYNNEKQAIGWA
Sbjct: 361 KAVFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWA 420

Query: 421 TANCDRVPKSRVSS 431
           TANCDRVPKS+VSS
Sbjct: 421 TANCDRVPKSQVSS 432

BLAST of Lag0007535 vs. ExPASy TrEMBL
Match: A0A6J1I590 (aspartic proteinase Asp1-like OS=Cucurbita maxima OX=3661 GN=LOC111469733 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 5.9e-239
Identity = 401/433 (92.61%), Postives = 413/433 (95.38%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVP--TASSSFASSSIVL 60
           MGKGVLMILVLMVSSI  SCLAPCSASSFFKDK WERRRP LSVP  +ASSS AS SIVL
Sbjct: 1   MGKGVLMILVLMVSSI--SCLAPCSASSFFKDKLWERRRPTLSVPIASASSSIASPSIVL 60

Query: 61  PLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSND 120
           PLQGNVFPNGFYNVTL+IGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSND
Sbjct: 61  PLQGNVFPNGFYNVTLFIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSND 120

Query: 121 LVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPR 180
           LVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPR
Sbjct: 121 LVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPR 180

Query: 181 LTLGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFF 240
           LTLGCGYDQ+PGSSSYHPMDG+LGLGKGAVSIVSQLHNQGI+RNVVGHCFSSKGGGYLFF
Sbjct: 181 LTLGCGYDQNPGSSSYHPMDGVLGLGKGAVSIVSQLHNQGIVRNVVGHCFSSKGGGYLFF 240

Query: 241 GDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300
           GDDIYD YRL WTPMSRDYPKHYSPGFG+L FNGRSTGLRNLFVVFDSGSSYTYFNAQAY
Sbjct: 241 GDDIYDPYRLAWTPMSRDYPKHYSPGFGDLFFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300

Query: 301 QVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKA 360
           Q++TSLLNREL GKPLREA+DDDTLPLCWRGR PFKSLRDVRKYFKPLALSF SG RSKA
Sbjct: 301 QIITSLLNRELTGKPLREAKDDDTLPLCWRGRNPFKSLRDVRKYFKPLALSFSSGRRSKA 360

Query: 361 VFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420
           VFE+P E YLIISS GNVCLGILNG+EVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA
Sbjct: 361 VFEMPTESYLIISSKGNVCLGILNGSEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420

Query: 421 NCDRVPKSRVSSM 432
           NCDRVPKS V S+
Sbjct: 421 NCDRVPKSSVGSL 431

BLAST of Lag0007535 vs. ExPASy TrEMBL
Match: A0A6J1G7F2 (aspartic proteinase Asp1-like OS=Cucurbita moschata OX=3662 GN=LOC111451545 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.1e-237
Identity = 400/433 (92.38%), Postives = 411/433 (94.92%), Query Frame = 0

Query: 1   MGKGVLMILVLMVSSISMSCLAPCSASSFFKDKPWERRRPILSVP--TASSSFASSSIVL 60
           MGKGVLMILV MV SI  SCLAPCSASSFFKDK WERRRP LSVP  +ASSS  SSSIVL
Sbjct: 8   MGKGVLMILVPMVFSI--SCLAPCSASSFFKDKLWERRRPTLSVPIASASSSIPSSSIVL 67

Query: 61  PLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSND 120
           PLQGNVFPNGFYNVTL+IGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSND
Sbjct: 68  PLQGNVFPNGFYNVTLFIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSND 127

Query: 121 LVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPR 180
           LVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPR
Sbjct: 128 LVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPR 187

Query: 181 LTLGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSKGGGYLFF 240
           LTLGCGYDQDPGSSSYHPMDG+LGLGKGAVSIVSQLHNQGI+RNVVGHCFSSKGGGYLFF
Sbjct: 188 LTLGCGYDQDPGSSSYHPMDGVLGLGKGAVSIVSQLHNQGIVRNVVGHCFSSKGGGYLFF 247

Query: 241 GDDIYDSYRLVWTPMSRDYPKHYSPGFGELLFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300
           GDDIYD YRL WTPMSRDYPKHYSPGFG+L FNGRSTGLRNLFVVFDSGSSYTYFNAQAY
Sbjct: 248 GDDIYDPYRLAWTPMSRDYPKHYSPGFGDLFFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 307

Query: 301 QVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFPSGGRSKA 360
           Q++TSLLNREL GKPLREA+DDDTLPLCWRGR PFKSLRDVRKYFKPLALSF SG RSKA
Sbjct: 308 QIITSLLNRELTGKPLREAKDDDTLPLCWRGRNPFKSLRDVRKYFKPLALSFSSGRRSKA 367

Query: 361 VFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420
           VFE+PME YLIISS GNVCLGILNG+EVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA
Sbjct: 368 VFEMPMESYLIISSKGNVCLGILNGSEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 427

Query: 421 NCDRVPKSRVSSM 432
            CDRVPKS V S+
Sbjct: 428 ICDRVPKSSVGSL 438

BLAST of Lag0007535 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 1.9e-168
Identity = 263/381 (69.03%), Postives = 320/381 (83.99%), Query Frame = 0

Query: 52  ASSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHP 111
           A SS+V P+ GNV+P G+YNVT+ IGQPP+PY+LD DTGSDLTWLQCDAPC +C E PHP
Sbjct: 42  AVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHP 101

Query: 112 LYQPSNDLVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTN 171
           LYQPS+DL+PC DPLC +LH + + RCE P+QCDYEVEYADGGSSLGVLVRD+F +N T 
Sbjct: 102 LYQPSSDLIPCNDPLCKALHLNSNQRCETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQ 161

Query: 172 GDPIRPRLTLGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSK 231
           G  + PRL LGCGYDQ PG+SS+HP+DG+LGLG+G VSI+SQLH+QG ++NV+GHC SS 
Sbjct: 162 GLRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSL 221

Query: 232 GGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGF-GELLFNGRSTGLRNLFVVFDSGSSY 291
           GGG LFFGDD+YDS R+ WTPMSR+Y KHYSP   GELLF GR+TGL+NL  VFDSGSSY
Sbjct: 222 GGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSY 281

Query: 292 TYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSF 351
           TYFN++AYQ +T LL REL+GKPL+EA DD TLPLCW+GR+PF S+ +V+KYFKPLALSF
Sbjct: 282 TYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSF 341

Query: 352 PSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNEK 411
            +G RSK +FEIP E YLIIS  GNVCLGILNGTE+GL+N N+IGDISMQD+M++Y+NEK
Sbjct: 342 KTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEK 401

Query: 412 QAIGWATANCDRVPKSRVSSM 432
           Q+IGW   +CD +   + + +
Sbjct: 402 QSIGWMPVDCDELASLKAAQV 422

BLAST of Lag0007535 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 547.4 bits (1409), Expect = 1.1e-155
Identity = 246/345 (71.30%), Postives = 293/345 (84.93%), Query Frame = 0

Query: 52  ASSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHP 111
           A SS+V P+ GNV+P G+YNVT+ IGQPP+PY+LD DTGSDLTWLQCDAPC +C E PHP
Sbjct: 39  AVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHP 98

Query: 112 LYQPSNDLVPCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTN 171
           LYQPS+DL+PC DPLC +LH + + RCE P+QCDYEVEYADGGSSLGVLVRD+F +N T 
Sbjct: 99  LYQPSSDLIPCNDPLCKALHLNSNQRCETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQ 158

Query: 172 GDPIRPRLTLGCGYDQDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSSK 231
           G  + PRL LGCGYDQ PG+SS+HP+DG+LGLG+G VSI+SQLH+QG ++NV+GHC SS 
Sbjct: 159 GLRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSL 218

Query: 232 GGGYLFFGDDIYDSYRLVWTPMSRDYPKHYSPGF-GELLFNGRSTGLRNLFVVFDSGSSY 291
           GGG LFFGDD+YDS R+ WTPMSR+Y KHYSP   GELLF GR+TGL+NL  VFDSGSSY
Sbjct: 219 GGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSY 278

Query: 292 TYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSF 351
           TYFN++AYQ +T LL REL+GKPL+EA DD TLPLCW+GR+PF S+ +V+KYFKPLALSF
Sbjct: 279 TYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSF 338

Query: 352 PSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIG 396
            +G RSK +FEIP E YLIIS  GNVCLGILNGTE+GL+N N+IG
Sbjct: 339 KTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIG 383

BLAST of Lag0007535 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 203/396 (51.26%), Postives = 287/396 (72.47%), Query Frame = 0

Query: 41  ILSVPTASSSF-------ASSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDL 100
           ++ VP + SS        + SS+V PL GNVFP G+Y+V + IG PPK +  D DTGSDL
Sbjct: 13  LVIVPLSKSSIFKTFIKSSPSSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDL 72

Query: 101 TWLQCDAPCQQCTETPHPLYQPSNDLVPCKDPLCMSLHSSIDHRCENP-DQCDYEVEYAD 160
           TW+QCDAPC  CT  P+  Y+P  +++PC +P+C +LH      C NP +QCDYEV+YAD
Sbjct: 73  TWVQCDAPCSGCTLPPNLQYKPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYAD 132

Query: 161 GGSSLGVLVRDIFPLNLTNGDPIRPRLTLGCGYDQDPGSSSYHPMD-GILGLGKGAVSIV 220
            GSS+G LV D FPL L NG  ++P +  GCGYDQ   S+   P   G+LGLG+G + ++
Sbjct: 133 QGSSMGALVTDQFPLKLVNGSFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLL 192

Query: 221 SQLHNQGIIRNVVGHCFSSKGGGYLFFGDDIYDSYRLVWTP-MSRDYPKHYSPGFGELLF 280
           +QL + G+ RNVVGHC SSKGGG+LFFGD++  S  + WTP +S+D   HY+ G  +LLF
Sbjct: 193 TQLVSAGLTRNVVGHCLSSKGGGFLFFGDNLVPSIGVAWTPLLSQD--NHYTTGPADLLF 252

Query: 281 NGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGR 340
           NG+ TGL+ L ++FD+GSSYTYFN++AYQ + +L+  +L   PL+ A++D TLP+CW+G 
Sbjct: 253 NGKPTGLKGLKLIFDTGSSYTYFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGA 312

Query: 341 KPFKSLRDVRKYFKPLALSFPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLEN 400
           KPFKS+ +V+ +FK + ++F +G R+  ++  P E YLI+S  GNVCLG+LNG+EVGL+N
Sbjct: 313 KPFKSVLEVKNFFKTITINFTNGRRNTQLYLAP-ELYLIVSKTGNVCLGLLNGSEVGLQN 372

Query: 401 SNIIGDISMQDKMVVYNNEKQAIGWATANCDRVPKS 427
           SN+IGDISMQ  M++Y+NEKQ +GW +++C+++PK+
Sbjct: 373 SNVIGDISMQGLMMIYDNEKQQLGWVSSDCNKLPKT 405

BLAST of Lag0007535 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 414.8 bits (1065), Expect = 8.2e-116
Identity = 198/377 (52.52%), Postives = 264/377 (70.03%), Query Frame = 0

Query: 53  SSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPL 112
           SS++V P+ GNV+P G+Y V L IG PPK + LD DTGSDLTW+QCDAPC  CT+     
Sbjct: 50  SSTVVFPVSGNVYPLGYYYVLLNIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQ 109

Query: 113 YQPSNDLVPCKDPLCMSLHSSIDHRCENP-DQCDYEVEYADGGSSLGVLVRDIFPLNLTN 172
           Y+P+++ +PC   LC  L    D  C +P DQCDYE+ Y+D  SS+G LV D  PL L N
Sbjct: 110 YKPNHNTLPCSHILCSGLDLPQDRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLAN 169

Query: 173 GDPIRPRLTLGCGYD-QDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSS 232
           G  +  RLT GCGYD Q+PG     P  GILGLG+G V + +QL + GI +NV+ HC S 
Sbjct: 170 GSIMNLRLTFGCGYDQQNPGPHPPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSH 229

Query: 233 KGGGYLFFGDDIYDSYRLVWTPMSRDYP-KHYSPGFGELLFNGRSTGLRNLFVVFDSGSS 292
            G G+L  GD++  S  + WT ++ + P K+Y  G  ELLFN ++TG++ + VVFDSGSS
Sbjct: 230 TGKGFLSIGDELVPSSGVTWTSLATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSS 289

Query: 293 YTYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALS 352
           YTYFNA+AYQ +  L+ ++L GKPL + +DD +LP+CW+G+KP KSL +V+KYFK + L 
Sbjct: 290 YTYFNAEAYQAILDLIRKDLNGKPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLR 349

Query: 353 FPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNE 412
           F    ++  +F++P E YLII+  G VCLGILNGTE+GLE  NIIGDIS Q  MV+Y+NE
Sbjct: 350 F-GNQKNGQLFQVPPESYLIITEKGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNE 409

Query: 413 KQAIGWATANCDRVPKS 427
           KQ IGW +++CD++PKS
Sbjct: 410 KQRIGWISSDCDKLPKS 425

BLAST of Lag0007535 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 411.4 bits (1056), Expect = 9.1e-115
Identity = 196/375 (52.27%), Postives = 262/375 (69.87%), Query Frame = 0

Query: 53  SSSIVLPLQGNVFPNGFYNVTLYIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPL 112
           SS++V P+ GNV+P G+Y V L IG PPK + LD DTGSDLTW+QCDAPC  CT+     
Sbjct: 50  SSTVVFPVSGNVYPLGYYYVLLNIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQ 109

Query: 113 YQPSNDLVPCKDPLCMSLHSSIDHRCENP-DQCDYEVEYADGGSSLGVLVRDIFPLNLTN 172
           Y+P+++ +PC   LC  L    D  C +P DQCDYE+ Y+D  SS+G LV D  PL L N
Sbjct: 110 YKPNHNTLPCSHILCSGLDLPQDRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLAN 169

Query: 173 GDPIRPRLTLGCGYD-QDPGSSSYHPMDGILGLGKGAVSIVSQLHNQGIIRNVVGHCFSS 232
           G  +  RLT GCGYD Q+PG     P  GILGLG+G V + +QL + GI +NV+ HC S 
Sbjct: 170 GSIMNLRLTFGCGYDQQNPGPHPPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSH 229

Query: 233 KGGGYLFFGDDIYDSYRLVWTPMSRDYP-KHYSPGFGELLFNGRSTGLRNLFVVFDSGSS 292
            G G+L  GD++  S  + WT ++ + P K+Y  G  ELLFN ++TG++ + VVFDSGSS
Sbjct: 230 TGKGFLSIGDELVPSSGVTWTSLATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSS 289

Query: 293 YTYFNAQAYQVLTSLLNRELAGKPLREAEDDDTLPLCWRGRKPFKSLRDVRKYFKPLALS 352
           YTYFNA+AYQ +  L+ ++L GKPL + +DD +LP+CW+G+KP KSL +V+KYFK + L 
Sbjct: 290 YTYFNAEAYQAILDLIRKDLNGKPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLR 349

Query: 353 FPSGGRSKAVFEIPMEGYLIISSMGNVCLGILNGTEVGLENSNIIGDISMQDKMVVYNNE 412
           F    ++  +F++P E YLII+  G VCLGILNGTE+GLE  NIIGDIS Q  MV+Y+NE
Sbjct: 350 F-GNQKNGQLFQVPPESYLIITEKGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNE 409

Query: 413 KQAIGWATANCDRVP 425
           KQ IGW +++CD++P
Sbjct: 410 KQRIGWISSDCDKLP 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900559.12.2e-24393.97aspartic proteinase Asp1 isoform X2 [Benincasa hispida][more]
XP_004147327.26.3e-24393.95aspartic proteinase Asp1 isoform X1 [Cucumis sativus] >KAE8651999.1 hypothetical... [more]
XP_008460823.19.1e-24293.49PREDICTED: aspartic proteinase Asp1 isoform X2 [Cucumis melo][more]
TYK02025.19.1e-24293.49aspartic proteinase Asp1 isoform X2 [Cucumis melo var. makuwa][more]
XP_008460822.15.0e-24092.63PREDICTED: aspartic proteinase Asp1 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q0IU525.6e-9347.68Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S... [more]
A2ZC672.4e-8845.34Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Q9M9A87.6e-8243.32Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q9S9K42.2e-3329.41Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q8VYV91.1e-2729.76Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3BS694.4e-24293.49Aspartic proteinase Asp1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3CDB24.4e-24293.49aspartic proteinase Asp1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4... [more]
A0A1S3CDB42.4e-24092.63aspartic proteinase Asp1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4... [more]
A0A6J1I5905.9e-23992.61aspartic proteinase Asp1-like OS=Cucurbita maxima OX=3661 GN=LOC111469733 PE=4 S... [more]
A0A6J1G7F21.1e-23792.38aspartic proteinase Asp1-like OS=Cucurbita moschata OX=3662 GN=LOC111451545 PE=4... [more]
Match NameE-valueIdentityDescription
AT4G33490.21.9e-16869.03Eukaryotic aspartyl protease family protein [more]
AT4G33490.11.1e-15571.30Eukaryotic aspartyl protease family protein [more]
AT1G44130.11.2e-12251.26Eukaryotic aspartyl protease family protein [more]
AT1G77480.28.2e-11652.52Eukaryotic aspartyl protease family protein [more]
AT1G77480.19.1e-11552.27Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 70..240
e-value: 4.5E-48
score: 163.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 241..424
e-value: 1.1E-27
score: 98.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 45..240
e-value: 1.1E-44
score: 154.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 62..423
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 281..416
e-value: 5.0E-15
score: 55.6
NoneNo IPR availablePANTHERPTHR13683:SF800EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 7..425
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..20
score: 5.0
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 7..425
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 70..416
score: 34.886223
IPR033823NucellinCDDcd05475nucellin_likecoord: 68..420
e-value: 4.821E-148
score: 419.854

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0007535.1Lag0007535.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity