Lag0006650 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0006650
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	TIR-NBS-LRR disease resistance protein
Location	chr6: 44525884 .. 44527132 (+)
RNA-Seq Expression	Lag0006650
Synteny	Lag0006650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCTCCCCTTGCTCTGGCTGAATCTTCATCTTCTTCTCCAAATTTGAAGTGGACTTATGATGTGTTTTTGAGTTTTAGAGGAGAGGATACAAGATCCAACTTCACCAGTCATCTTGACATGGCCTTGCGTCAAAAGGGAGTCTACTTCTTCATAGATGACAAGCTCGACAGAGGAGGGCAAATTTCTGAATCTCTTCTCAAATGTATTGAAGGATCTAGGATTTCCATTATTATATTTTCTGAAAATTATGCATCTTCCACTTGGTGTTTGGATGAACTGGTCAAAATCATTGAGTGTATGAAATCCAAGGGACAGTTAGTTTTACCAGTCTTCTACAAGGTGGATCCATCCGAAGTTCGAAAACAAACTGGTAGATTTGGTGATGAAACATGAAGCTAAGTTTATGAGCACCAAGATTCAAACATGGAAGGAAGCTTTGATTACTGCTGCTGGTTTGTCTGGTTGGGATCTAGCAACTTATAGGTCTTTCTCTGGAGACTCTGTCTTTTCAATTCCCTCTTTTTTGTTTAACAAGCACACTACCTACTAGATACAATCCTAATGATAAAATTGGCAAATTTTAAATCTTGAAAGGTTCAATAACCAGATTCAATTTAACTTTATCTACTATTGTCTATGTTGTTCATCACCTTTTTATTCATGTATGATATTTTTTAACGACAGGAAGGAAGCTGATGTTATCCAGGACCTTGTTAATAAAGTGTTGTCTAAAGTAAATCGCACTCAATTACTTGATGTAGCCAAGCATCCGATTGGAATTGATTCTCAACTAAGAGCTATTGAGTTGGCCTCCCATGTTGTGTCCCATGGTGTTTATATGGTAGGGCTATATGGCATGGGAGGCATTGGTAAGACGACTTTGGCCAAAGCTTTATACAACAAAATTGCGAATCAATTTGAAGGTTGCAGCTTTCTTTCAAATGTTAGAGAAGCTTCAGAGCAATTCAATGGTCTAGTTCAACTACAGGAAAATCTACTCTATGCAATCTTAAAGGATAATTTGAAGGTTGGCAATGTCGACAGAGGAATAAATATCATTAGGGATAGACTACGTTCAAAGAAAGTTCTTATAGTTCTAGATGATGTAGATAAGCTCGATCAATTAGAAAAATTGGTTGGTGGACGTGATTGGTTCAGTCAAGGTAGCAAAATCATTGTGACAACAAGGAATAAACAGTTACTTTCTGGCCATGGATTTGATAAAATACATGATATTTGTTAA

mRNA sequence

ATGGGTTCTCCCCTTGCTCTGGCTGAATCTTCATCTTCTTCTCCAAATTTGAAGTGGACTTATGATGTGTTTTTGAGTTTTAGAGGAGAGGATACAAGATCCAACTTCACCAGTCATCTTGACATGGCCTTGCGTCAAAAGGGAGTCTACTTCTTCATAGATGACAAGCTCGACAGAGGAGGGCAAATTTCTGAATCTCTTCTCAAATGTATTGAAGGATCTAGGATTTCCATTATTATATTTTCTGAAAATTATGCATCTTCCACTTGGTGTTTGGATGAACTGGTCAAAATCATTGAGTATTTGGTGATGAAACATGAAGCTAAGTTTATGAGCACCAAGATTCAAACATGGAAGGAAGCTTTGATTACTGCTGCTGGTTTGTCTGGTTGGGATCTAGCAACTTATAGGAAGGAAGCTGATGTTATCCAGGACCTTGTTAATAAAGTGTTGTCTAAAGTAAATCGCACTCAATTACTTGATGTAGCCAAGCATCCGATTGGAATTGATTCTCAACTAAGAGCTATTGAGTTGGCCTCCCATGTTGTGTCCCATGGTGTTTATATGGTAGGGCTATATGGCATGGGAGGCATTGGTAAGACGACTTTGGCCAAAGCTTTATACAACAAAATTGCGAATCAATTTGAAGGTTGCAGCTTTCTTTCAAATGTTAGAGAAGCTTCAGAGCAATTCAATGGTCTAGTTCAACTACAGGAAAATCTACTCTATGCAATCTTAAAGGATAATTTGAAGGTTGGCAATGTCGACAGAGGAATAAATATCATTAGGGATAGACTACGTTCAAAGAAAGTTCTTATAGTTCTAGATGATGTAGATAAGCTCGATCAATTAGAAAAATTGGTTGGTGGACGTGATTGGTTCAGTCAAGGTAGCAAAATCATTGTGACAACAAGGAATAAACAGTTACTTTCTGGCCATGGATTTGATAAAATACATGATATTTGTTAA

Coding sequence (CDS)

ATGGGTTCTCCCCTTGCTCTGGCTGAATCTTCATCTTCTTCTCCAAATTTGAAGTGGACTTATGATGTGTTTTTGAGTTTTAGAGGAGAGGATACAAGATCCAACTTCACCAGTCATCTTGACATGGCCTTGCGTCAAAAGGGAGTCTACTTCTTCATAGATGACAAGCTCGACAGAGGAGGGCAAATTTCTGAATCTCTTCTCAAATGTATTGAAGGATCTAGGATTTCCATTATTATATTTTCTGAAAATTATGCATCTTCCACTTGGTGTTTGGATGAACTGGTCAAAATCATTGAGTATTTGGTGATGAAACATGAAGCTAAGTTTATGAGCACCAAGATTCAAACATGGAAGGAAGCTTTGATTACTGCTGCTGGTTTGTCTGGTTGGGATCTAGCAACTTATAGGAAGGAAGCTGATGTTATCCAGGACCTTGTTAATAAAGTGTTGTCTAAAGTAAATCGCACTCAATTACTTGATGTAGCCAAGCATCCGATTGGAATTGATTCTCAACTAAGAGCTATTGAGTTGGCCTCCCATGTTGTGTCCCATGGTGTTTATATGGTAGGGCTATATGGCATGGGAGGCATTGGTAAGACGACTTTGGCCAAAGCTTTATACAACAAAATTGCGAATCAATTTGAAGGTTGCAGCTTTCTTTCAAATGTTAGAGAAGCTTCAGAGCAATTCAATGGTCTAGTTCAACTACAGGAAAATCTACTCTATGCAATCTTAAAGGATAATTTGAAGGTTGGCAATGTCGACAGAGGAATAAATATCATTAGGGATAGACTACGTTCAAAGAAAGTTCTTATAGTTCTAGATGATGTAGATAAGCTCGATCAATTAGAAAAATTGGTTGGTGGACGTGATTGGTTCAGTCAAGGTAGCAAAATCATTGTGACAACAAGGAATAAACAGTTACTTTCTGGCCATGGATTTGATAAAATACATGATATTTGTTAA

Protein sequence

MGSPLALAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYLVMKHEAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRTQLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGNVDRGINIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDIC

Homology

[Close]

BLAST of Lag0006650 vs. NCBI nr
Match: KGN51651.2 (hypothetical protein Csa_008503 [Cucumis sativus])

HSP 1 Score: 435.6 bits (1119), Expect = 3.6e-118
Identity = 240/355 (67.61%), Postives = 273/355 (76.90%), Query Frame = 0


Query: 1   MGSPL-ALAESSSS-SPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLD 60
           MGS L  LA+SSSS S NLKW+YDVFLSFRGEDTR+NFTSHLD ALR+KGV FFIDDKL+
Sbjct: 1   MGSSLVGLAQSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLE 60

Query: 61  RGGQISESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL---------------- 120
           RGGQISESLLK I+GS+ISIIIFS+NYASSTWCLDELVKI++ +                
Sbjct: 61  RGGQISESLLKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDP 120

Query: 121 -------------VMKHEA-KFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVN 180
                        + KHEA + M+ K+Q WKEAL TAA LSGWDLAT + EAD+I DLV 
Sbjct: 121 SEVRKQTGGFGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVK 180

Query: 181 KVLSKVNRTQLLDVAKHPIGIDSQLRAI-ELASHVVSHGVYMVGLYGMGGIGKTTLAKAL 240
           +VLS +N+TQLL VAKHP+GIDSQLRA+ ELASH V  GV MVG++GMGGIGKTTLAKAL
Sbjct: 181 EVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKAL 240

Query: 241 YNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNL-KVGNVDRGINIIRDRL 300
           YNKIA QFE C FLSNVRE  EQF  LVQLQE LL  ILKDN  KVGNV +G NIIRDRL
Sbjct: 241 YNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRL 300

Query: 301 RSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
            SKKVLI+LDDVDK +QL+ LVG RDWF +GSKII TTR++ LL  H FD ++ I
Sbjct: 301 CSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPI 355

[Close]

BLAST of Lag0006650 vs. NCBI nr
Match: XP_031741747.1 (TMV resistance protein N [Cucumis sativus])

HSP 1 Score: 435.6 bits (1119), Expect = 3.6e-118
Identity = 240/355 (67.61%), Postives = 273/355 (76.90%), Query Frame = 0


Query: 1   MGSPL-ALAESSSS-SPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLD 60
           MGS L  LA+SSSS S NLKW+YDVFLSFRGEDTR+NFTSHLD ALR+KGV FFIDDKL+
Sbjct: 1   MGSSLVGLAQSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLE 60

Query: 61  RGGQISESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL---------------- 120
           RGGQISESLLK I+GS+ISIIIFS+NYASSTWCLDELVKI++ +                
Sbjct: 61  RGGQISESLLKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDP 120

Query: 121 -------------VMKHEA-KFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVN 180
                        + KHEA + M+ K+Q WKEAL TAA LSGWDLAT + EAD+I DLV 
Sbjct: 121 SEVRKQTGGFGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVK 180

Query: 181 KVLSKVNRTQLLDVAKHPIGIDSQLRAI-ELASHVVSHGVYMVGLYGMGGIGKTTLAKAL 240
           +VLS +N+TQLL VAKHP+GIDSQLRA+ ELASH V  GV MVG++GMGGIGKTTLAKAL
Sbjct: 181 EVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKAL 240

Query: 241 YNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNL-KVGNVDRGINIIRDRL 300
           YNKIA QFE C FLSNVRE  EQF  LVQLQE LL  ILKDN  KVGNV +G NIIRDRL
Sbjct: 241 YNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRL 300

Query: 301 RSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
            SKKVLI+LDDVDK +QL+ LVG RDWF +GSKII TTR++ LL  H FD ++ I
Sbjct: 301 CSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPI 355

[Close]

BLAST of Lag0006650 vs. NCBI nr
Match: XP_011648798.1 (TMV resistance protein N [Cucumis sativus] >KGN60871.1 hypothetical protein Csa_023462 [Cucumis sativus])

HSP 1 Score: 424.5 bits (1090), Expect = 8.3e-115
Identity = 229/367 (62.40%), Postives = 270/367 (73.57%), Query Frame = 0


Query: 1   MGSPLALAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRG 60
           MGS  A  ES +     +W+YDVFLSFRGEDTR+NFTSHLDMALRQKGV  FIDDKL+RG
Sbjct: 1   MGSSTAATESMA----FEWSYDVFLSFRGEDTRTNFTSHLDMALRQKGVNVFIDDKLERG 60

Query: 61  GQISESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIE-------------------- 120
            QISESL K I+ + ISI+IFS+NYASS+WCLDELV IIE                    
Sbjct: 61  EQISESLFKSIQEASISIVIFSQNYASSSWCLDELVNIIECKKSKGQNVFPVFYKVDPSD 120

Query: 121 ---------YLVMKHEAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVL 180
                      + KH+ KF  TK Q W++AL TAA LSGWDL  YR+EAD+I+DLV +VL
Sbjct: 121 IRKQTGSFGEAMAKHQPKF-QTKTQIWRKALTTAANLSGWDLGAYRREADLIRDLVKEVL 180

Query: 181 SKVNRTQL-LDVAKHPIGIDSQLRAIELASHVVSH----------------GVYMVGLYG 240
           S +NRT+  L VAK+P+GIDSQL  ++  SH ++                 GVYMVG+YG
Sbjct: 181 STINRTRTPLYVAKYPVGIDSQLEYMKFHSHHLNKGNKFQYWTQNEYESDIGVYMVGIYG 240

Query: 241 MGGIGKTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGN 300
           +GG+GKTTLAKALYNKIA+QFEGC FLSNVR+AS QFNGLVQLQ+NLLY IL+D+LK  N
Sbjct: 241 IGGLGKTTLAKALYNKIASQFEGCCFLSNVRQASNQFNGLVQLQQNLLYEILEDDLKFVN 300

Query: 301 VDRGINIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHG 322
           +D+GI IIR+RLRSKKVLIVLDDVDKL+QLE LVGGRDWF QGSKIIVTTRN  LLS HG
Sbjct: 301 LDKGITIIRNRLRSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNSHLLSSHG 360

[Close]

BLAST of Lag0006650 vs. NCBI nr
Match: XP_016901470.1 (PREDICTED: LOW QUALITY PROTEIN: disease resistance protein RPP4-like [Cucumis melo])

HSP 1 Score: 422.9 bits (1086), Expect = 2.4e-114
Identity = 233/362 (64.36%), Postives = 268/362 (74.03%), Query Frame = 0


Query: 7   LAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 66
           L +SS+ S   KW+YDVFLSFRGEDTR+NFTSHLDMALRQKGV  FIDDKL+RG QISES
Sbjct: 25  LMDSSTESSTFKWSYDVFLSFRGEDTRTNFTSHLDMALRQKGVNVFIDDKLERGEQISES 84

Query: 67  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIE-------------------------- 126
           L K I+ + ISI+IFS+NYASS+WCLDELVKIIE                          
Sbjct: 85  LFKSIQEALISIVIFSQNYASSSWCLDELVKIIECKKSKGQIVLPIFYKVDPSDIRKQSG 144

Query: 127 ---YLVMKHEAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRT 186
                + KH+AKF  TK Q W+EAL TAA LSGWDL T RKEAD+I DLV  VLS +NRT
Sbjct: 145 TFGEALAKHQAKF-QTKTQIWREALTTAANLSGWDLGT-RKEADLIGDLVKNVLSTLNRT 204

Query: 187 -QLLDVAKHPIGIDSQLRAIELASHVV-----------------SHGVYMVGLYGMGGIG 246
              L VAK+P+GIDS+L  ++L SH +                   GVY+VG+YG+GGIG
Sbjct: 205 CTPLYVAKYPVGIDSKLEYMKLQSHNLFEKSNKFHYQTQHEYEFDTGVYLVGIYGIGGIG 264

Query: 247 KTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGNVDRGI 306
           KTTLAKALYNKIA+QFEGC FLSNVREAS+QFNGL QLQE+LLY IL  +LKV N+DRGI
Sbjct: 265 KTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQESLLYEILTVDLKVVNIDRGI 324

Query: 307 NIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIH 322
           NIIR+RL SKKVLIVLDDVDKL+QLE LVGGRDWF QGS+I+VTTRNK LLS HGFD+IH
Sbjct: 325 NIIRNRLCSKKVLIVLDDVDKLEQLETLVGGRDWFGQGSRIMVTTRNKHLLSSHGFDEIH 384

[Close]

BLAST of Lag0006650 vs. NCBI nr
Match: TYK06440.1 (TMV resistance protein N [Cucumis melo var. makuwa])

HSP 1 Score: 419.1 bits (1076), Expect = 3.5e-113
Identity = 232/323 (71.83%), Postives = 263/323 (81.42%), Query Frame = 0


Query: 8   AESSSS-SPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 67
           AESSSS S NL+W+YDVFLSFRGEDTR  F SHL +ALRQKGV FFIDDKLDRG QIS+S
Sbjct: 9   AESSSSCSSNLRWSYDVFLSFRGEDTRDKFISHLVVALRQKGVNFFIDDKLDRGHQISKS 68

Query: 68  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL---VMKHEA-KFMSTKIQTWKEAL 127
           LLK IE SRISIIIFS+NYASSTWCLDELVKII      + K+E  + M  KIQ WKEAL
Sbjct: 69  LLKSIEESRISIIIFSQNYASSTWCLDELVKIIRVFGEAMAKYETNRLMINKIQPWKEAL 128

Query: 128 ITAAGLSGWDLATY-RKEADVIQDLVNKVLSKVNRTQLLDVAKHPIGIDSQLRAI-ELAS 187
            TAA LSGWDL  Y + EA +I DLV KV S + +TQLL+VAKHP+GIDSQL+A+ E AS
Sbjct: 129 TTAATLSGWDLPNYWKNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFAS 188

Query: 188 H-VVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQE 247
           H V+ +GV MVG++GMGGIGKTTLAKALYNKI  +FE C FLSNVREASEQFNGLVQLQE
Sbjct: 189 HGVLDNGVNMVGIHGMGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQE 248

Query: 248 NLLYAILKD-NLKVGNVDRGINIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGS 307
            LL  I KD NLKV NV +G+NI++DRL S+KVLIVLDDVDK DQL+ LVGGRDWF +GS
Sbjct: 249 KLLSEIFKDNNLKVDNVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGS 308

Query: 308 KIIVTTRNKQLLSGHGFDKIHDI 322
           KIIVTTR++ LL  + FDKIH I
Sbjct: 309 KIIVTTRDRHLLERYSFDKIHPI 330

[Close]

BLAST of Lag0006650 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.4e-69
Identity = 155/346 (44.80%), Postives = 213/346 (61.56%), Query Frame = 0


Query: 8   AESSSSSPNL--KWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISE 67
           A SSSS+P++    TYDVFLSFRGEDTR NFT HL  AL ++G+  F DD+L RG  I+ 
Sbjct: 9   ASSSSSTPSIPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRDDRLRRGEAIAP 68

Query: 68  SLLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL------------------VMKHE 127
            LLK IE SR S+I+FSENYA S WCLDELVKI+E                    V K E
Sbjct: 69  ELLKAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQE 128

Query: 128 AKF----------MSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRT 187
             F             KI  W+ AL  AA LSGW L   R E++ I+++ N +  ++ + 
Sbjct: 129 GSFGEAFAGYEENWKDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQL-KC 188

Query: 188 QLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEG 247
           + LDV  + +GIDS ++ + L  H+ S  V MVG+YG+GGIGKTT+AK +YN+++ +FE 
Sbjct: 189 KRLDVGANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEY 248

Query: 248 CSFLSNVREASEQFNGLVQLQENLLYAIL--KDNLKVGNVDRGINIIRDRLRSKKVLIVL 307
            SFL N+RE S     L  LQ  LL  IL  + +  + +V    ++I+D L S++V IVL
Sbjct: 249 MSFLENIREGSNP-QVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVL 308

Query: 308 DDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
           DDVD L QLE L+G R+W  +GS++I+TTRNK +L+    D ++++
Sbjct: 309 DDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQEVDDLYEV 352

[Close]

BLAST of Lag0006650 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.7e-65
Identity = 153/351 (43.59%), Postives = 211/351 (60.11%), Query Frame = 0


Query: 8   AESSSSSPNL--KWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISE 67
           + SSSS+P++    TYDVFLSFRGEDTR NFT HL  AL ++G+  F DDKL RG  I+ 
Sbjct: 12  SSSSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRDDKLRRGEAIAP 71

Query: 68  SLLKCIEGSRISIIIFSENYASSTWCLDELVKIIE---------------------YLVM 127
            LLK IE SR S+I+FSENYA S WCLDELVKI+E                       V 
Sbjct: 72  ELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHVR 131

Query: 128 KHEAKF----------MSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKV 187
           K E  F          +  KI  W+ AL  AA LSGW L     E++ I+++ + +  ++
Sbjct: 132 KQEGSFGEAFAGYGENLKDKIPRWRTALTEAANLSGWPLQD-GYESNQIKEITDSIFRRL 191

Query: 188 NRTQLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQ 247
            + + LD   + +GIDS ++ +    H+ S  V MVG+YG+GGIGKTT+AK +YN+++ +
Sbjct: 192 -KCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSRE 251

Query: 248 FEGCSFLSNVREASEQFN--GLVQLQENLLYAILK--DNLKVGNVDRGINIIRDRLRSKK 307
           FE  SFL N+R   E+FN  G+  LQ  LL  ILK   +  + +V  G ++I+D L SK 
Sbjct: 252 FEYMSFLENIR---EKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDILSSKI 311

Query: 308 VLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
           V IVLDDVD   QLE L+  R+W  +GS++I+TTRNK +L     D ++++
Sbjct: 312 VFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVDDLYEV 357

[Close]

BLAST of Lag0006650 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.0e-58
Identity = 140/349 (40.11%), Postives = 204/349 (58.45%), Query Frame = 0


Query: 12  SSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDK-LDRGGQISESLLKC 71
           +SS +   +YDVFLSFRGEDTR  F  HL  AL +KG++ F+DDK L RG  IS  L+K 
Sbjct: 3   TSSSHHGRSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKA 62

Query: 72  IEGSRISIIIFSENYASSTWCLDELVKII---------------------------EYLV 131
           I  SR ++++FS+NYASSTWCL+ELVKI+                           EY V
Sbjct: 63  IGESRFAVVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAV 122

Query: 132 --MKHEAKFMS--TKIQTWKEALITAAGLSGWDLA-TYRKE-----ADVIQDLVNKVLSK 191
              K EA  +    K+  W+EAL   A +SG DL  TY  +       +++D+ +K    
Sbjct: 123 CFTKFEANLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFS 182

Query: 192 VNRTQLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIAN 251
           ++ T      +  +GI+SQ++ +     +   GV +VG++GMGG+GKTT A+AL+N+   
Sbjct: 183 ISITN-----RDLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQ 242

Query: 252 QFEGCSFLSNVREASEQFNGLVQLQENLLYAILK-DNLKVGNVDRGINIIRDRLRSKKVL 311
            FE   FL +V+E   Q + L+ LQ+ LL  +LK + +   + +    I++ RL SKKVL
Sbjct: 243 NFESACFLEDVKEYL-QHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVL 302

Query: 312 IVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
           +VLDDV+  DQL+KLVG  DWF  GS+I++TTR+ +LL  H   + ++I
Sbjct: 303 VVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHDVHETYEI 345

[Close]

BLAST of Lag0006650 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 140/343 (40.82%), Postives = 198/343 (57.73%), Query Frame = 0


Query: 7   LAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDK-LDRGGQISE 66
           +A SSSSS   +W+YDVFLSFRGEDTR  FTSHL   L  KG+  F DDK L+ G  I  
Sbjct: 1   MASSSSSS---RWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPG 60

Query: 67  SLLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYLVM--------------------- 126
            L K IE S+ +I++FSENYA+S WCL+ELVKI+E                         
Sbjct: 61  ELCKAIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQK 120

Query: 127 --------KHEAKFMS--TKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKV 186
                   +HE K+      IQ W+ AL  AA L G      + +AD I+ +V+++ SK+
Sbjct: 121 ESFAKAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKL 180

Query: 187 NRTQLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKI--- 246
            +   L   ++ +GID+ L  IE    +  +GV ++G++GMGG+GKTT+A+A+++ +   
Sbjct: 181 CKIS-LSYLQNIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGR 240

Query: 247 ---ANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGNVDRGINIIRDRLRS 306
              + QF+G  FL +++E      G+  LQ  LL  +L++     N + G + +  RLRS
Sbjct: 241 MDSSYQFDGACFLKDIKENK---RGMHSLQNALLSELLREKANYNNEEDGKHQMASRLRS 300

Query: 307 KKVLIVLDDVDKLDQ-LEKLVGGRDWFSQGSKIIVTTRNKQLL 311
           KKVLIVLDD+D  D  LE L G  DWF  GS+II+TTR+K L+
Sbjct: 301 KKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLI 336

[Close]

BLAST of Lag0006650 vs. ExPASy Swiss-Prot
Match: O82500 (Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana OX=3702 GN=At4g11170 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.0e-51
Identity = 128/350 (36.57%), Postives = 190/350 (54.29%), Query Frame = 0


Query: 7   LAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 66
           +A SSS+S    W YDVF SFRGED R+NF SHL      KG+  F DD + R   I   
Sbjct: 1   MASSSSNS----WRYDVFPSFRGEDVRNNFLSHLLKEFESKGIVTFRDDHIKRSHTIGHE 60

Query: 67  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL------------------VMKHEA 126
           L   I  S+IS+++FSENYASS+WCLDEL++I++                    + K   
Sbjct: 61  LRAAIRESKISVVLFSENYASSSWCLDELIEIMKCKEEQGLKVMPVFYKVDPSDIRKQTG 120

Query: 127 KFMSTKIQT-----------WKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRT 186
           KF  + ++T           W+ AL  AA + G     +  EA  I  +   VL K+N T
Sbjct: 121 KFGMSFLETCCGKTEERQHNWRRALTDAANILGDHPQNWDNEAYKITTISKDVLEKLNAT 180

Query: 187 QLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEG 246
              D     +G+++ +  +E    + S GV +VG++G  G+GKTT+A+ALYN+    F  
Sbjct: 181 PSRDF-NDLVGMEAHIAKMESLLCLESQGVRIVGIWGPAGVGKTTIARALYNQYHENFNL 240

Query: 247 CSFLSNVREA-----SEQFNGLVQLQENLLYAIL-KDNLKVGNVDRGINIIRDRLRSKKV 306
             F+ NVRE+      + +   + LQ+  L  +L + +L+V    R +  I +RL+S+KV
Sbjct: 241 SIFMENVRESYGEAGLDDYGLKLHLQQRFLSKLLDQKDLRV----RHLGAIEERLKSQKV 300

Query: 307 LIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
           LI+LDDVD ++QL+ L     WF   S+I+VTT+NKQLL  H  + ++ +
Sbjct: 301 LIILDDVDNIEQLKALAKENQWFGNKSRIVVTTQNKQLLVSHDINHMYQV 341

[Close]

BLAST of Lag0006650 vs. ExPASy TrEMBL
Match: A0A0A0KNK0 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G494390 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.7e-118
Identity = 240/355 (67.61%), Postives = 273/355 (76.90%), Query Frame = 0


Query: 1   MGSPL-ALAESSSS-SPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLD 60
           MGS L  LA+SSSS S NLKW+YDVFLSFRGEDTR+NFTSHLD ALR+KGV FFIDDKL+
Sbjct: 1   MGSSLVGLAQSSSSCSSNLKWSYDVFLSFRGEDTRNNFTSHLDRALREKGVNFFIDDKLE 60

Query: 61  RGGQISESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL---------------- 120
           RGGQISESLLK I+GS+ISIIIFS+NYASSTWCLDELVKI++ +                
Sbjct: 61  RGGQISESLLKSIDGSKISIIIFSKNYASSTWCLDELVKIVQCMKSMGHIVFPVFYKVDP 120

Query: 121 -------------VMKHEA-KFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVN 180
                        + KHEA + M+ K+Q WKEAL TAA LSGWDLAT + EAD+I DLV 
Sbjct: 121 SEVRKQTGGFGEALAKHEANELMTNKVQPWKEALTTAASLSGWDLATRKNEADLIHDLVK 180

Query: 181 KVLSKVNRTQLLDVAKHPIGIDSQLRAI-ELASHVVSHGVYMVGLYGMGGIGKTTLAKAL 240
           +VLS +N+TQLL VAKHP+GIDSQLRA+ ELASH V  GV MVG++GMGGIGKTTLAKAL
Sbjct: 181 EVLSILNQTQLLHVAKHPVGIDSQLRAVEELASHDVPDGVNMVGIHGMGGIGKTTLAKAL 240

Query: 241 YNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNL-KVGNVDRGINIIRDRL 300
           YNKIA QFE C FLSNVRE  EQF  LVQLQE LL  ILKDN  KVGNV +G NIIRDRL
Sbjct: 241 YNKIAYQFEACCFLSNVRETLEQFKDLVQLQEKLLSEILKDNAWKVGNVHKGKNIIRDRL 300

Query: 301 RSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
            SKKVLI+LDDVDK +QL+ LVG RDWF +GSKII TTR++ LL  H FD ++ I
Sbjct: 301 CSKKVLIILDDVDKDEQLDALVGERDWFGRGSKIIATTRDRHLLENHSFDIVYPI 355

[Close]

BLAST of Lag0006650 vs. ExPASy TrEMBL
Match: A0A0A0LLL0 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G020940 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 4.0e-115
Identity = 229/367 (62.40%), Postives = 270/367 (73.57%), Query Frame = 0


Query: 1   MGSPLALAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRG 60
           MGS  A  ES +     +W+YDVFLSFRGEDTR+NFTSHLDMALRQKGV  FIDDKL+RG
Sbjct: 1   MGSSTAATESMA----FEWSYDVFLSFRGEDTRTNFTSHLDMALRQKGVNVFIDDKLERG 60

Query: 61  GQISESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIE-------------------- 120
            QISESL K I+ + ISI+IFS+NYASS+WCLDELV IIE                    
Sbjct: 61  EQISESLFKSIQEASISIVIFSQNYASSSWCLDELVNIIECKKSKGQNVFPVFYKVDPSD 120

Query: 121 ---------YLVMKHEAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVL 180
                      + KH+ KF  TK Q W++AL TAA LSGWDL  YR+EAD+I+DLV +VL
Sbjct: 121 IRKQTGSFGEAMAKHQPKF-QTKTQIWRKALTTAANLSGWDLGAYRREADLIRDLVKEVL 180

Query: 181 SKVNRTQL-LDVAKHPIGIDSQLRAIELASHVVSH----------------GVYMVGLYG 240
           S +NRT+  L VAK+P+GIDSQL  ++  SH ++                 GVYMVG+YG
Sbjct: 181 STINRTRTPLYVAKYPVGIDSQLEYMKFHSHHLNKGNKFQYWTQNEYESDIGVYMVGIYG 240

Query: 241 MGGIGKTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGN 300
           +GG+GKTTLAKALYNKIA+QFEGC FLSNVR+AS QFNGLVQLQ+NLLY IL+D+LK  N
Sbjct: 241 IGGLGKTTLAKALYNKIASQFEGCCFLSNVRQASNQFNGLVQLQQNLLYEILEDDLKFVN 300

Query: 301 VDRGINIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHG 322
           +D+GI IIR+RLRSKKVLIVLDDVDKL+QLE LVGGRDWF QGSKIIVTTRN  LLS HG
Sbjct: 301 LDKGITIIRNRLRSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNSHLLSSHG 360

[Close]

BLAST of Lag0006650 vs. ExPASy TrEMBL
Match: A0A1S4E0G6 (LOW QUALITY PROTEIN: disease resistance protein RPP4-like OS=Cucumis melo OX=3656 GN=LOC103499468 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.2e-114
Identity = 233/362 (64.36%), Postives = 268/362 (74.03%), Query Frame = 0


Query: 7   LAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 66
           L +SS+ S   KW+YDVFLSFRGEDTR+NFTSHLDMALRQKGV  FIDDKL+RG QISES
Sbjct: 25  LMDSSTESSTFKWSYDVFLSFRGEDTRTNFTSHLDMALRQKGVNVFIDDKLERGEQISES 84

Query: 67  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIE-------------------------- 126
           L K I+ + ISI+IFS+NYASS+WCLDELVKIIE                          
Sbjct: 85  LFKSIQEALISIVIFSQNYASSSWCLDELVKIIECKKSKGQIVLPIFYKVDPSDIRKQSG 144

Query: 127 ---YLVMKHEAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRT 186
                + KH+AKF  TK Q W+EAL TAA LSGWDL T RKEAD+I DLV  VLS +NRT
Sbjct: 145 TFGEALAKHQAKF-QTKTQIWREALTTAANLSGWDLGT-RKEADLIGDLVKNVLSTLNRT 204

Query: 187 -QLLDVAKHPIGIDSQLRAIELASHVV-----------------SHGVYMVGLYGMGGIG 246
              L VAK+P+GIDS+L  ++L SH +                   GVY+VG+YG+GGIG
Sbjct: 205 CTPLYVAKYPVGIDSKLEYMKLQSHNLFEKSNKFHYQTQHEYEFDTGVYLVGIYGIGGIG 264

Query: 247 KTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGNVDRGI 306
           KTTLAKALYNKIA+QFEGC FLSNVREAS+QFNGL QLQE+LLY IL  +LKV N+DRGI
Sbjct: 265 KTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQESLLYEILTVDLKVVNIDRGI 324

Query: 307 NIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIH 322
           NIIR+RL SKKVLIVLDDVDKL+QLE LVGGRDWF QGS+I+VTTRNK LLS HGFD+IH
Sbjct: 325 NIIRNRLCSKKVLIVLDDVDKLEQLETLVGGRDWFGQGSRIMVTTRNKHLLSSHGFDEIH 384

[Close]

BLAST of Lag0006650 vs. ExPASy TrEMBL
Match: A0A5D3C521 (TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001460 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.7e-113
Identity = 232/323 (71.83%), Postives = 263/323 (81.42%), Query Frame = 0


Query: 8   AESSSS-SPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 67
           AESSSS S NL+W+YDVFLSFRGEDTR  F SHL +ALRQKGV FFIDDKLDRG QIS+S
Sbjct: 9   AESSSSCSSNLRWSYDVFLSFRGEDTRDKFISHLVVALRQKGVNFFIDDKLDRGHQISKS 68

Query: 68  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYL---VMKHEA-KFMSTKIQTWKEAL 127
           LLK IE SRISIIIFS+NYASSTWCLDELVKII      + K+E  + M  KIQ WKEAL
Sbjct: 69  LLKSIEESRISIIIFSQNYASSTWCLDELVKIIRVFGEAMAKYETNRLMINKIQPWKEAL 128

Query: 128 ITAAGLSGWDLATY-RKEADVIQDLVNKVLSKVNRTQLLDVAKHPIGIDSQLRAI-ELAS 187
            TAA LSGWDL  Y + EA +I DLV KV S + +TQLL+VAKHP+GIDSQL+A+ E AS
Sbjct: 129 TTAATLSGWDLPNYWKNEAHLIHDLVEKV-SILKQTQLLNVAKHPVGIDSQLKAVEEFAS 188

Query: 188 H-VVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEGCSFLSNVREASEQFNGLVQLQE 247
           H V+ +GV MVG++GMGGIGKTTLAKALYNKI  +FE C FLSNVREASEQFNGLVQLQE
Sbjct: 189 HGVLDNGVNMVGIHGMGGIGKTTLAKALYNKITYEFEACCFLSNVREASEQFNGLVQLQE 248

Query: 248 NLLYAILKD-NLKVGNVDRGINIIRDRLRSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGS 307
            LL  I KD NLKV NV +G+NI++DRL S+KVLIVLDDVDK DQL+ LVGGRDWF +GS
Sbjct: 249 KLLSEIFKDNNLKVDNVHKGMNIMKDRLCSRKVLIVLDDVDKDDQLDALVGGRDWFGRGS 308

Query: 308 KIIVTTRNKQLLSGHGFDKIHDI 322
           KIIVTTR++ LL  + FDKIH I
Sbjct: 309 KIIVTTRDRHLLERYSFDKIHPI 330

[Close]

BLAST of Lag0006650 vs. ExPASy TrEMBL
Match: A0A0A0LWV1 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G334920 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.8e-113
Identity = 238/350 (68.00%), Postives = 268/350 (76.57%), Query Frame = 0


Query: 8   AESSSS-SPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 67
           AESSSS S N KW+YDVFLSFRGEDTR  F SHLD+ALR++GV FFIDDKLDRG QIS+S
Sbjct: 9   AESSSSCSSNSKWSYDVFLSFRGEDTRDKFISHLDLALRREGVNFFIDDKLDRGKQISKS 68

Query: 68  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIEYLVMKHE------------------- 127
           LLK IEGSRISIIIFS+NYASSTWCLDE+VKIIE +  K +                   
Sbjct: 69  LLKSIEGSRISIIIFSQNYASSTWCLDEVVKIIECMRSKKQTVLPVFYNVSPSEVVKQTG 128

Query: 128 ------AKF-----MSTKIQTWKEALITAAGLSGWDLATYRK--EADVIQDLVNKVLSKV 187
                 AK+     M+ KIQ WKEAL TAA LSGWDL  Y K  EA +IQDLV KV S +
Sbjct: 129 IFGEAFAKYETNPLMTNKIQPWKEALTTAATLSGWDLGNYWKNNEAHLIQDLVKKV-SIL 188

Query: 188 NRTQLLDVAKHPIGIDSQLRAI-ELASHVVS-HGVYMVGLYGMGGIGKTTLAKALYNKIA 247
            +TQLL+VAKHP+ IDSQL+AI ELASH VS +GV MVG++GMGGIGKTTLAKALYNKI 
Sbjct: 189 KQTQLLNVAKHPVAIDSQLKAIEELASHGVSDNGVNMVGIHGMGGIGKTTLAKALYNKIT 248

Query: 248 NQFEGCSFLSNVREASEQFNGLVQLQENLLYAILKD-NLKVGNVDRGINIIRDRLRSKKV 307
            QFE C FLSNVRE SEQFNGLVQLQE LL  I KD NLKV NVD+G+NII+DRL S+KV
Sbjct: 249 YQFEACCFLSNVRETSEQFNGLVQLQEKLLNEIFKDNNLKVDNVDKGMNIIKDRLCSRKV 308

Query: 308 LIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
           L+VLDDVDK DQL+ LVGGRDWF +GSKIIVTTR++ LL  + FDKIH I
Sbjct: 309 LMVLDDVDKDDQLDALVGGRDWFGRGSKIIVTTRDRHLLETYSFDKIHPI 357

[Close]

BLAST of Lag0006650 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 229.9 bits (585), Expect = 2.8e-60
Identity = 138/338 (40.83%), Postives = 198/338 (58.58%), Query Frame = 0


Query: 5   LALAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDD-KLDRGGQI 64
           L +A+     P  +WTYDVF+SFRG D R NF SHL  +LR+ G+  F+DD +L RG  I
Sbjct: 2   LGMAQGRERIPE-RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYI 61

Query: 65  SESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIE-------YLVM------------ 124
           S  LL  IE S+I I++ +++YASS WCLDELV I++       ++V             
Sbjct: 62  SPELLNAIETSKILIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIR 121

Query: 125 -----------KHEAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSK 184
                      KH+      K++ W+EAL   A +SGWD+   R EA+ I D+  ++L +
Sbjct: 122 WQQGSYAKSFSKHKNSHPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKR 181

Query: 185 VNRTQLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIAN 244
           +   Q L V  + +G+ S+L+ I     + S GV ++ +YGMGGIGKTTLAK  +N+ ++
Sbjct: 182 L-PCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSH 241

Query: 245 QFEGCSFLSNVREASEQFNGLVQLQENLLYAILKDN-LKVGNVDRGINIIRDRLRSKKVL 304
            FEG SFL N RE S++  G   LQ  LL  IL+ N ++   +D   + +++R RSK+VL
Sbjct: 242 LFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLD---HAVKERFRSKRVL 301

Query: 305 IVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLL 311
           +V+DDVD + QL      RD F  GS+II+TTRN  LL
Sbjct: 302 LVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLL 333

[Close]

BLAST of Lag0006650 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 229.6 bits (584), Expect = 3.7e-60
Identity = 135/325 (41.54%), Postives = 193/325 (59.38%), Query Frame = 0


Query: 18  KWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDD-KLDRGGQISESLLKCIEGSRI 77
           +WTYDVF+SFRG D R NF SHL  +LR+ G+  F+DD +L RG  IS  LL  IE S+I
Sbjct: 11  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 70

Query: 78  SIIIFSENYASSTWCLDELVKIIE-------YLVM-----------------------KH 137
            I++ +++YASS WCLDELV I++       ++V                        KH
Sbjct: 71  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 130

Query: 138 EAKFMSTKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRTQLLDVAKHP 197
           +      K++ W+EAL   A +SGWD+   R EA+ I D+  ++L ++   Q L V  + 
Sbjct: 131 KNSHPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRL-PCQYLHVPSYA 190

Query: 198 IGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEGCSFLSNVRE 257
           +G+ S+L+ I     + S GV ++ +YGMGGIGKTTLAK  +N+ ++ FEG SFL N RE
Sbjct: 191 VGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFRE 250

Query: 258 ASEQFNGLVQLQENLLYAILKDN-LKVGNVDRGINIIRDRLRSKKVLIVLDDVDKLDQLE 311
            S++  G   LQ  LL  IL+ N ++   +D   + +++R RSK+VL+V+DDVD + QL 
Sbjct: 251 YSKKPEGRTHLQHQLLSDILRRNDIEFKGLD---HAVKERFRSKRVLLVVDDVDDVHQLN 310

[Close]

BLAST of Lag0006650 vs. TAIR 10
Match: AT5G46450.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 216.5 bits (550), Expect = 3.2e-56
Identity = 127/356 (35.67%), Postives = 201/356 (56.46%), Query Frame = 0


Query: 7   LAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISES 66
           +A SSSSS N  W+YDVF SF GED R  F SH    L +K +  F D++++R   I+  
Sbjct: 1   MASSSSSSRN--WSYDVFPSFSGEDVRKTFLSHFLRELERKSIITFKDNEMERSQSIAPE 60

Query: 67  LLKCIEGSRISIIIFSENYASSTWCLDELVKIIE--------------YLVMKH------ 126
           L++ I+ SRI++I+FS+NYASS+WCL+EL++I+               YL   H      
Sbjct: 61  LVEAIKDSRIAVIVFSKNYASSSWCLNELLEIMRCNKYLGQQVIPVFYYLDPSHLRKQSG 120

Query: 127 ---EAKFMSTKIQT------WKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRT 186
              EA   + + QT      WK+AL   + + G+       EA +I+++ + +L K++ T
Sbjct: 121 EFGEAFKKTCQNQTEEVKNQWKQALTDVSNILGYHSKNCNSEATMIEEISSHILGKLSLT 180

Query: 187 QLLDVAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEG 246
              D  +  +GI   +  + L  H+ S  V MVG++G  GIGKTT+A+AL++ +++QF+ 
Sbjct: 181 PSNDFEEF-VGIKDHIEKVRLLLHLESDEVRMVGIWGTSGIGKTTIARALFSNLSSQFQS 240

Query: 247 CSFLSNV----------REASEQFNGLVQLQENLLYAIL-KDNLKVGNVDRGINIIRDRL 306
             ++             R   + +N  ++L+EN L+ IL K N+K+G ++       +RL
Sbjct: 241 SVYIDRAFISKSMEGYGRANPDDYNMKLRLRENFLFEILGKKNMKIGAME-------ERL 300

Query: 307 RSKKVLIVLDDVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDIC 323
           + +KVLI++DD+D  D L+ LVG   WF  GS+IIV T+NK  L  HG D +++ C
Sbjct: 301 KHQKVLIIIDDLDDQDVLDALVGRTQWFGSGSRIIVVTKNKHFLRAHGIDHVYEAC 346

[Close]

BLAST of Lag0006650 vs. TAIR 10
Match: AT1G27170.1 (transmembrane receptors;ATP binding )

HSP 1 Score: 215.7 bits (548), Expect = 5.5e-56
Identity = 128/335 (38.21%), Postives = 191/335 (57.01%), Query Frame = 0


Query: 9   ESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDDKLDRGGQISESLL 68
           ES   S   +  YDVFLSFRG DTR NF  HL  AL+ K   F  ++ ++RG +IS SL 
Sbjct: 2   ESGVVSKPHRLKYDVFLSFRGADTRDNFGDHLYKALKDKVRVFRDNEGMERGDEISSSLK 61

Query: 69  KCIEGSRISIIIFSENYASSTWCLDELVKI-----------------------------I 128
             +E S  S+I+ S NY+ S WCLDEL  +                             I
Sbjct: 62  AGMEDSAASVIVISRNYSGSRWCLDELAMLCKMKSSLDRRILPIFYHVDPSHVRKQSDHI 121

Query: 129 EYLVMKHEAKFM--STKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRT 188
           +    +H+ +F     K+Q W+EAL     L+G+      K+ D+I+ +V +VL++++ T
Sbjct: 122 KKDFEEHQVRFSEEKEKVQEWREALTLVGNLAGYVCDKDSKDDDMIELVVKRVLAELSNT 181

Query: 189 QLLDVAKHPIGIDSQLRAIE-LASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFE 248
               V +  +G++S L+ +  L     S GV ++GLYGMGGIGKTTLAKA YNKI   FE
Sbjct: 182 P-EKVGEFIVGLESPLKDLTGLIDTESSSGVQVLGLYGMGGIGKTTLAKAFYNKIVGNFE 241

Query: 249 GCSFLSNVREASEQFNGLVQLQENLLYAILKDNLKVGNVDRGINIIRDRLRSKKVLIVLD 308
             +F+S++RE S   NGLV LQ+ L+  + +   ++ +V  G+  I+  +  KK+++VLD
Sbjct: 242 QRAFISDIRERSSAENGLVTLQKTLIKELFRLVPEIEDVSIGLEKIKANVHEKKIIVVLD 301

Query: 309 DVDKLDQLEKLVGGRDWFSQGSKIIVTTRNKQLLS 312
           DVD +DQ+  LVG   W+ QG+ I++TTR+ ++LS
Sbjct: 302 DVDHIDQVHALVGETRWYGQGTLIVITTRDSEILS 335

[Close]

BLAST of Lag0006650 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 213.4 bits (542), Expect = 2.7e-55
Identity = 131/341 (38.42%), Postives = 194/341 (56.89%), Query Frame = 0


Query: 6   ALAESSSSSPNLKWTYDVFLSFRGEDTRSNFTSHLDMALRQKGVYFFIDD-KLDRGGQIS 65
           +L  SSSSS +  W  DVF+SFRGED R  F SHL     + G+  F DD  L RG  IS
Sbjct: 3   SLPSSSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSIS 62

Query: 66  ESLLKCIEGSRISIIIFSENYASSTWCLDELVKIIE----------YLVMKHEAKFM--- 125
             L+  I+GSR +I++ S NYA+S+WCLDEL+KI+E          Y V   + +     
Sbjct: 63  PELIDAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNKDTIVPIFYEVDPSDVRRQRGS 122

Query: 126 ----------STKIQTWKEALITAAGLSGWDLATYRKEADVIQDLVNKVLSKVNRTQLLD 185
                       K+  WKEAL   A +SG D   +  ++ +I+ +V  +  K+  T   D
Sbjct: 123 FGEDVESHSDKEKVGKWKEALKKLAAISGEDSRNW-DDSKLIKKIVKDISDKLVSTS-WD 182

Query: 186 VAKHPIGIDSQLRAIELASHVVSHGVYMVGLYGMGGIGKTTLAKALYNKIANQFEGCSFL 245
            +K  IG+ S +  ++    +V   V M+G++GMGG+GKTT+AK LYN+++ QF+   F+
Sbjct: 183 DSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFM 242

Query: 246 SNVREASEQFNGLVQLQENLLYAILKDNLK-VGNVDRGINIIRDRLRSKKVLIVLDDVDK 305
            NV+E   ++ G+ +LQ   L  + ++  K   +     NII++R R K V IVLDDVD+
Sbjct: 243 ENVKEVCNRY-GVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDR 302

Query: 306 LDQLEKLVGGRDWFSQGSKIIVTTRNKQLLSGHGFDKIHDI 322
            +QL +LV    WF  GS+IIVTTR++ LL  HG + ++ +
Sbjct: 303 SEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKV 340

The following BLAST results are available for this feature:

BLAST of Lag0006650 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN51651.2	3.6e-118	67.61	hypothetical protein Csa_008503 [Cucumis sativus]	[more]
XP_031741747.1	3.6e-118	67.61	TMV resistance protein N [Cucumis sativus]	[more]
XP_011648798.1	8.3e-115	62.40	TMV resistance protein N [Cucumis sativus] >KGN60871.1 hypothetical protein Csa_...	[more]
XP_016901470.1	2.4e-114	64.36	PREDICTED: LOW QUALITY PROTEIN: disease resistance protein RPP4-like [Cucumis me...	[more]
TYK06440.1	3.5e-113	71.83	TMV resistance protein N [Cucumis melo var. makuwa]	[more]

BLAST of Lag0006650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
V9M2S5	1.4e-69	44.80	Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=...	[more]
V9M398	1.7e-65	43.59	Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=...	[more]
A0A290U7C4	2.0e-58	40.11	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	1.3e-57	40.82	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
O82500	1.0e-51	36.57	Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Lag0006650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNK0	1.7e-118	67.61	TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G494390 PE=4 SV...	[more]
A0A0A0LLL0	4.0e-115	62.40	TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G020940 PE=4 SV...	[more]
A0A1S4E0G6	1.2e-114	64.36	LOW QUALITY PROTEIN: disease resistance protein RPP4-like OS=Cucumis melo OX=365...	[more]
A0A5D3C521	1.7e-113	71.83	TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0LWV1	3.8e-113	68.00	TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G334920 PE=4 SV...	[more]

BLAST of Lag0006650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36930.2	2.8e-60	40.83	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G36930.1	3.7e-60	41.54	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G46450.1	3.2e-56	35.67	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT1G27170.1	5.5e-56	38.21	transmembrane receptors;ATP binding	[more]
AT5G17680.1	2.7e-55	38.42	disease resistance protein (TIR-NBS-LRR class), putative	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00364	DISEASERSIST	coord: 189..204 score: 67.5 coord: 265..279 score: 57.5
None	No IPR available	PANTHER	PTHR11017:SF422	RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATED	coord: 15..98
None	No IPR available	PANTHER	PTHR11017:SF422	RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATED	coord: 105..315
None	No IPR available	CDD	cd00009	AAA	coord: 192..306 e-value: 3.61918E-4 score: 38.2811
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 20..128 e-value: 8.3E-25 score: 98.4
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 21..103 e-value: 5.8E-28 score: 97.9
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 19..154 score: 30.600349
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 18..101 e-value: 1.1E-36 score: 127.9
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 102..153 e-value: 1.2E-9 score: 40.2
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 11..131
IPR002182	NB-ARC	PFAM	PF00931	NB-ARC	coord: 186..310 e-value: 4.5E-17 score: 62.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 154..316 e-value: 1.1E-31 score: 111.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 151..316
IPR044974	Disease resistance protein, plants	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 105..315
IPR044974	Disease resistance protein, plants	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 15..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0006650.1	Lag0006650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0080156	mitochondrial mRNA modification
biological_process	GO:0007165	signal transduction
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0003953	NAD+ nucleosidase activity