Lag0006485 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0006485 vs. NCBI nr
Match: KAA0048111.1 (pectinesterase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 257.7 bits (657), Expect = 7.6e-65
Identity = 135/175 (77.14%), Postives = 150/175 (85.71%), Query Frame = 0

Query: 7   LASFIPALLLAVVFLTIFPSHAILHQNLVTICSKTSNPSLCQKNLGNDPQTVTADLPKLS 66
           L SFIPALLLA   LTIFPSH I H+NLVTICSKTSNPSLC+K L ND +TV+ADLPKLS
Sbjct: 7   LTSFIPALLLATFSLTIFPSHGIPHENLVTICSKTSNPSLCEKILNNDSRTVSADLPKLS 66

Query: 67  LISINLTMKQVDQNLDTFSKLRKNKSDSEQKKSFEHCVGYYHEIQTNIQKAYEFSQQQMF 126
           LI  NL  KQ DQNLDTF KL KN+SD E KKSFEHCV YYHEIQ+NIQKAY+FSQQ++F
Sbjct: 67  LICSNLAKKQADQNLDTFYKLSKNESDPEVKKSFEHCVEYYHEIQSNIQKAYQFSQQKIF 126

Query: 127 RDNGPLLASKKLVLGCSQAIRVNSLQIEVMNKLMILSCETSISVNQYAAANGYFQ 182
           R+NGPL+ASK+LVL CSQAIR+NSL IEVMNKLMIL C+ SISVNQ AAANG+ Q
Sbjct: 127 RENGPLVASKRLVLRCSQAIRINSLHIEVMNKLMILCCDISISVNQCAAANGHIQ 181

BLAST of Lag0006485 vs. NCBI nr
Match: KAE8651630.1 (hypothetical protein Csa_021165 [Cucumis sativus])

HSP 1 Score: 254.6 bits (649), Expect = 6.4e-64
Identity = 132/173 (76.30%), Postives = 150/173 (86.71%), Query Frame = 0

Query: 7   LASFIPALLLAVVFLTIFPSHAILHQNLVTICSKTSNPSLCQKNLGNDPQTVTADLPKLS 66
           L SFIPALLLA   LT+FPSH I H+NLVTICSKTSNPSLC+K L ND +TV+A+LPKLS
Sbjct: 496 LTSFIPALLLAAFSLTVFPSHGIPHENLVTICSKTSNPSLCEKILNNDSRTVSANLPKLS 555

Query: 67  LISINLTMKQVDQNLDTFSKLRKNKSDSEQKKSFEHCVGYYHEIQTNIQKAYEFSQQQMF 126
           LI  NL  KQ DQNLDTF KL KN+SD E+KKSFEHCV YYHEIQ+NIQKAY+FSQQ++F
Sbjct: 556 LICSNLAKKQADQNLDTFYKLSKNESDPEEKKSFEHCVKYYHEIQSNIQKAYQFSQQKIF 615

Query: 127 RDNGPLLASKKLVLGCSQAIRVNSLQIEVMNKLMILSCETSISVNQYAAANGY 180
           R+NGPL+ASK+LVL CSQAIR+NSL IEVMNKLMIL C+ SISVNQ AAANG+
Sbjct: 616 RENGPLVASKRLVLKCSQAIRINSLYIEVMNKLMILCCDISISVNQCAAANGH 668

BLAST of Lag0006485 vs. NCBI nr
Match: KAG6599172.1 (hypothetical protein SDJN03_08950, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030110.1 hypothetical protein SDJN02_08457, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 226.9 bits (577), Expect = 1.4e-55
Identity = 117/171 (68.42%), Postives = 138/171 (80.70%), Query Frame = 0

Query: 11  IPALLLAVVFLTIFPSHAILHQNLVTICSKTSNPSLCQKNLGNDPQTVTADLPKLSLISI 70
           IP LLLAV +L IFPSH ILH NL TIC KT +P  CQ+ LGN P T++ADLPKLSLISI
Sbjct: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66

Query: 71  NLTMKQVDQNLDTFSKLRKNKSDSEQKKSFEHCVGYYHEIQTNIQKAYEFSQQQMFRDNG 130
           NLT  Q DQNLD+FSKL KN+SD E KKSF+HCV YYHEIQ +IQKA++ S++++FRDN 
Sbjct: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126

Query: 131 PLLASKKLVLGCSQAIRVNSLQIEVMNKLMILSCETSISVNQYAAANGYFQ 182
           PL+ SKKLV  C+QAIR+NSL IEVMNK MILSC+ S+SVN YA AN + Q
Sbjct: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ 177

BLAST of Lag0006485 vs. NCBI nr
Match: KAB1201343.1 (Pectinesterase inhibitor [Morella rubra] >KAB1224060.1 Pectinesterase inhibitor [Morella rubra])

HSP 1 Score: 137.9 bits (346), Expect = 8.7e-29
Identity = 74/165 (44.85%), Postives = 106/165 (64.24%), Query Frame = 0

Query: 11  IPALLLAVVFLTIFPSHAILHQNLVTICSKTSNPSLCQKNLGNDPQTVTADLPKLSLISI 70
           +   +L+V    I+PS  + H  L TICSKT N   C+++L +D +T +ADLPKLSLISI
Sbjct: 8   VSVTVLSVASFLIYPSSGVSHDILTTICSKTQNHDACERSLESDSRTRSADLPKLSLISI 67

Query: 71  NLTMKQVDQNLDTFSKLRKNKSDSEQKKSFEHCVGYYHEIQTNIQKAYEFSQQQMFRDNG 130
            LTMKQ D N  TFS LR N +D   + SF +CVG+Y E+Q  I+ AY  SQ++ ++   
Sbjct: 68  ELTMKQADHNHRTFSDLRDNATDPRLEDSFRNCVGFYKEMQAQIRAAYHLSQKRQYQKIT 127

Query: 131 PLLASKKLVLGCSQAIRVNSLQIEVMNKLMILSCETSISVNQYAA 176
            L+ S++L   C+  I +N L+I+ +   M+L+ +TS SVNQY A
Sbjct: 128 ELVQSERLAQECASGIPINCLEIDALTTTMLLAIKTSESVNQYIA 172

BLAST of Lag0006485 vs. NCBI nr
Match: XP_007031213.2 (PREDICTED: pectinesterase inhibitor [Theobroma cacao])

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-25
Identity = 72/172 (41.86%), Postives = 104/172 (60.47%), Query Frame = 0

Query: 7   LASFIP---ALLLAVVFLTIFPSHAILHQNLVTICSKTSNPSLCQKNLGNDPQTVTADLP 66
           +AS+ P   AL L V F  I PS ++ ++ LV ICSKT NP LC+  L  DP+T +ADLP
Sbjct: 1   MASYTPRLLALTLLVAFSVIHPSSSVPNETLVAICSKTYNPILCESILRGDPRTSSADLP 60

Query: 67  KLSLISINLTMKQVDQNLDTFSKLRKNKSDSEQKKSFEHCVGYYHEIQTNIQKAYEFSQQ 126
           +LSL+SINLT++Q   N   F +   N +D   KK F  CV  Y  ++ N+ KAY  S+ 
Sbjct: 61  ELSLVSINLTIEQAANNSQVFVEFHDNTTDPALKKGFNSCVELYQLMEDNLDKAYHRSEG 120

Query: 127 QMFRDNGPLLASKKLVLGCSQAIRVNSLQIEVMNKLMILSCETSISVNQYAA 176
             +R+   L+ S    + C+ A+ +N + I+ + + ++  CETSISVNQY A
Sbjct: 121 GDYRNITELVQSSNQAVECASALAINLVTIDALTRAVVSKCETSISVNQYIA 172

BLAST of Lag0006485 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.1e-05
Identity = 28/79 (35.44%), Postives = 45/79 (56.96%), Query Frame = 0

Query: 39  SKTSNPSLCQKNLGNDPQTVTADLPKLSLISINLTMKQVDQNLDTFSKLRKNKSDSEQKK 98
           S   N   C K+LG DP++ +ADL  L +IS NL ++Q  +      ++ K+K D   KK
Sbjct: 29  SPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQTFIGRILKSKVDPALKK 88

Query: 99  SFEHCVGYYHEIQTNIQKA 118
               CVG Y + ++++Q+A
Sbjct: 89  YLNDCVGLYADAKSSVQEA 107

BLAST of Lag0006485 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.4e-05
Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 0

Query: 39  SKTSNPSLCQKNLGNDPQTVTADLPKLSLISINLTMKQVDQNLDTFSKLRKNKSDSEQKK 98
           S   N   C K+LG DP++ TADL  L +IS NL ++   +      ++ K+K D   KK
Sbjct: 38  SPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIGRILKSKVDPALKK 97

Query: 99  SFEHCVGYYHEIQTNIQKA 118
               CVG Y + ++++Q+A
Sbjct: 98  YLNDCVGLYADAKSSVQEA 116

BLAST of Lag0006485 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 7.0e-05
Identity = 35/123 (28.46%), Postives = 60/123 (48.78%), Query Frame = 0

Query: 1   MGYSQILASFIPALLLAVVFLTIF---PSHAILHQNLVTICSKTSNPSLCQKNLGNDPQT 60
           M +S   +S   +LLL ++F++     PS    +  +  IC KT NPSLC + L +DP++
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRS 60

Query: 61  VTADLPKLSLISINLTMKQVDQNLDTFSKLRKNKSDSEQKKSFEHCVGYYHEIQTNIQKA 120
            + DL  L   SI++      Q     + L    +D + K  +E C   Y +   ++ +A
Sbjct: 61  ASKDLKGLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQA 120