Lag0006201 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006201
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDUF4283 domain-containing protein
Locationchr6: 38951471 .. 38952148 (-)
RNA-Seq ExpressionLag0006201
SyntenyLag0006201
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAATTTTAACCACTGAAAAGATAGAAAGAAAATTCTTCTCCTGTTGCTTTGACAAGAACTTCAAAGGGAGAGTGGTTAGGATCTGTGAGACTCACCTGAAAAGAAGATTCACTCTATCCGCTGAGGAAATCATTCTTGTTTGGATCACTGATGTGATAGAGGACCTCCTTCTTTCCCCAAACACACACGAGTTCTCTCGGAAAACGGACTGTGTCAATGGGTTCATATGGATTCAGAAAGTTCTAAACAAGAGAGGGAGCTTCATAGAAATAACAAAAGTGAGTAATGTAGGAGGGAAGCATAACCTGGTAATTCCTGTGGGAGCAGATTTTAACGGACGGAGGGACTTTGCCAGGCTGCTGAAAGACTTTCTGAACGGAAATGAGGAGGAACACCATGTGGTTAACGCTCATAAGCTAAAAGAAGGGACGTCATATGCGAAGATTGTGAAGTATAACTTATTCGTTTCGGAAAGGAAGATGAGCAAAATGGAACGTGGAAATGAGAGAACAACATTTGGGTTGAAACCCTACCAATGGAAAGTCAGGAAAATAGACTGGGATGAAGTTATAGTGGTTACTAAAAGAGACTTCCACGATGACTTGGGTAGAATTCTCGAGATTCTCCAAGAACAAACAATAGCACTTCATTATAAACCCATTTCAAGAGGATAA

mRNA sequence

ATGATAATTTTAACCACTGAAAAGATAGAAAGAAAATTCTTCTCCTGTTGCTTTGACAAGAACTTCAAAGGGAGAGTGGTTAGGATCTGTGAGACTCACCTGAAAAGAAGATTCACTCTATCCGCTGAGGAAATCATTCTTGTTTGGATCACTGATGTGATAGAGGACCTCCTTCTTTCCCCAAACACACACGAGTTCTCTCGGAAAACGGACTGTGTCAATGGGTTCATATGGATTCAGAAAGTTCTAAACAAGAGAGGGAGCTTCATAGAAATAACAAAAGTGAGTAATGTAGGAGGGAAGCATAACCTGGTAATTCCTGTGGGAGCAGATTTTAACGGACGGAGGGACTTTGCCAGGCTGCTGAAAGACTTTCTGAACGGAAATGAGGAGGAACACCATGTGGTTAACGCTCATAAGCTAAAAGAAGGGACGTCATATGCGAAGATTGTGAAGTATAACTTATTCGTTTCGGAAAGGAAGATGAGCAAAATGGAACGTGGAAATGAGAGAACAACATTTGGGTTGAAACCCTACCAATGGAAAGTCAGGAAAATAGACTGGGATGAAGTTATAGTGGTTACTAAAAGAGACTTCCACGATGACTTGGGTAGAATTCTCGAGATTCTCCAAGAACAAACAATAGCACTTCATTATAAACCCATTTCAAGAGGATAA

Coding sequence (CDS)

ATGATAATTTTAACCACTGAAAAGATAGAAAGAAAATTCTTCTCCTGTTGCTTTGACAAGAACTTCAAAGGGAGAGTGGTTAGGATCTGTGAGACTCACCTGAAAAGAAGATTCACTCTATCCGCTGAGGAAATCATTCTTGTTTGGATCACTGATGTGATAGAGGACCTCCTTCTTTCCCCAAACACACACGAGTTCTCTCGGAAAACGGACTGTGTCAATGGGTTCATATGGATTCAGAAAGTTCTAAACAAGAGAGGGAGCTTCATAGAAATAACAAAAGTGAGTAATGTAGGAGGGAAGCATAACCTGGTAATTCCTGTGGGAGCAGATTTTAACGGACGGAGGGACTTTGCCAGGCTGCTGAAAGACTTTCTGAACGGAAATGAGGAGGAACACCATGTGGTTAACGCTCATAAGCTAAAAGAAGGGACGTCATATGCGAAGATTGTGAAGTATAACTTATTCGTTTCGGAAAGGAAGATGAGCAAAATGGAACGTGGAAATGAGAGAACAACATTTGGGTTGAAACCCTACCAATGGAAAGTCAGGAAAATAGACTGGGATGAAGTTATAGTGGTTACTAAAAGAGACTTCCACGATGACTTGGGTAGAATTCTCGAGATTCTCCAAGAACAAACAATAGCACTTCATTATAAACCCATTTCAAGAGGATAA

Protein sequence

MIILTTEKIERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSRKTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNGNEEEHHVVNAHKLKEGTSYAKIVKYNLFVSERKMSKMERGNERTTFGLKPYQWKVRKIDWDEVIVVTKRDFHDDLGRILEILQEQTIALHYKPISRG
Homology
BLAST of Lag0006201 vs. NCBI nr
Match: XP_031737554.1 (transcription factor Pur-alpha 1-like [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 7.8e-27
Identity = 63/134 (47.01%), Postives = 83/134 (61.94%), Query Frame = 0

Query: 8   KIERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFS 67
           KIE K FSC FD N +GRV+R+ E HL + +TL  EE +  W+ + +E++L +    +F 
Sbjct: 10  KIENKSFSCEFDANSRGRVIRLTEAHLNKSYTLLGEETLFGWLAESLEEVLQTSKPQKFF 69

Query: 68  RKTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLN 127
           RKT C  GF WIQK  NKRGSF+EI+KV   G K N+V+P G D  G   F RLL DFLN
Sbjct: 70  RKTFCNGGFTWIQKTTNKRGSFLEISKVLKSGKKGNIVVPAGVDLKGWESFRRLLMDFLN 129

Query: 128 GNEEEHHVVNAHKL 142
              +   + +  KL
Sbjct: 130 DEVDSRALHSEKKL 143

BLAST of Lag0006201 vs. NCBI nr
Match: KAG6601809.1 (hypothetical protein SDJN03_07042, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-14
Identity = 44/99 (44.44%), Postives = 61/99 (61.62%), Query Frame = 0

Query: 8   KIERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFS 67
           KIE + FSC F     G   +I E H+ R F ++ EEI+  W  D++EDLLL+P +    
Sbjct: 7   KIENRAFSCWFHNEKHGEYTKIKEKHIGRFFKVTVEEIVF-WPVDILEDLLLAPPSLRVF 66

Query: 68  RKTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVI 107
           RK  C   FIW+ ++ NKRGSFI++TKV + GGK  L +
Sbjct: 67  RKPVCNGRFIWLHRIKNKRGSFIQVTKVQSSGGKGTLYL 104

BLAST of Lag0006201 vs. NCBI nr
Match: KAA0041398.1 (hypothetical protein E6C27_scaffold206G00440 [Cucumis melo var. makuwa])

HSP 1 Score: 82.0 bits (201), Expect = 7.1e-12
Identity = 64/230 (27.83%), Postives = 110/230 (47.83%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           +E+K F    DK  +   + I E    + F+++     L W+    + LL +P T  F  
Sbjct: 13  VEKKDFVLSVDKRSRESNLLITEVGPYKSFSIAITLDSLEWLKTTFKALLNTPRTTRFFV 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNG 128
           +   V+  +W+QK+ N+RG   EI +V + G K  +++P G D  G   F+ +L      
Sbjct: 73  EKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFSDMLTCKKTS 132

Query: 129 NEEE---HHVVNAHKLKE---------------GTSYAKIVKYNLFVSERKMSKMERGNE 188
           +++E    H  N  K KE                 +YA++V  +   SE   SK +  + 
Sbjct: 133 DKKEISTRHYYNQDKGKEKIQKPYDSSTDSESPRKTYAEVVS-SFSSSESDYSKAKNTSS 192

Query: 189 RTTFGLKPYQWKVRK------IDWDEVIVVTKRDFHDDLGRILEILQEQT 215
              F L   + ++RK      IDW++ I++++R FHDD  +I++ L+EQT
Sbjct: 193 LKRF-LPAMKSEIRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQT 240

BLAST of Lag0006201 vs. NCBI nr
Match: KAA0063662.1 (hypothetical protein E6C27_scaffold329G001780 [Cucumis melo var. makuwa])

HSP 1 Score: 79.3 bits (194), Expect = 4.6e-11
Identity = 62/228 (27.19%), Postives = 107/228 (46.93%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           +E+K      DK  +   + I E    + F+++     L W+    + LL +P T  F  
Sbjct: 13  VEKKDSVLSVDKRSRESNLLITEVGPYKSFSIAITPDSLEWLKTTFKALLNTPRTTRFFV 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNG 128
           +   V+  +W+QK+ N+RG   EI +V + G K  +++P G D  G   F  +L      
Sbjct: 73  EKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFNDMLTCKKTS 132

Query: 129 NEEE---HHVVNAHKLKE---------GTSYAKIVKYNLFVSERKMSKMERGNERTTFGL 188
           +++E    H  N  K KE           S +    Y   VS    S+ +    ++T  L
Sbjct: 133 DKKEISTRHFYNQDKGKEKIQKPYDSSTDSESPRKTYAEVVSSFSSSESDYSKAKSTSSL 192

Query: 189 KPY----QWKVRK------IDWDEVIVVTKRDFHDDLGRILEILQEQT 215
           K +    + ++RK      IDW++ I++++R FHDD  +I++ L+EQT
Sbjct: 193 KRFLPALKSEIRKEERKNDIDWEKTIILSRRCFHDDWAKIIDRLREQT 240

BLAST of Lag0006201 vs. NCBI nr
Match: TYK18363.1 (hypothetical protein E5676_scaffold456G00820 [Cucumis melo var. makuwa])

HSP 1 Score: 79.3 bits (194), Expect = 4.6e-11
Identity = 62/228 (27.19%), Postives = 107/228 (46.93%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           +E+K      DK  +   + I E    + F+++     L W+    + LL +P T  F  
Sbjct: 13  VEKKDSVLSVDKRSRESNLLITEVGPYKSFSIAITPDSLEWLKTTFKALLNTPRTTRFFV 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNG 128
           +   V+  +W+QK+ N+RG   EI +V + G K  +++P G D  G   F  +L      
Sbjct: 73  EKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFNDMLTCKKTS 132

Query: 129 NEEE---HHVVNAHKLKE---------GTSYAKIVKYNLFVSERKMSKMERGNERTTFGL 188
           +++E    H  N  K KE           S +    Y   VS    S+ +    ++T  L
Sbjct: 133 DKKEISTRHFYNQDKGKEKIQKPYDSSTDSESPKKTYAEVVSSFSSSESDYSKAKSTSSL 192

Query: 189 KPY----QWKVRK------IDWDEVIVVTKRDFHDDLGRILEILQEQT 215
           K +    + ++RK      IDW++ I++++R FHDD  +I++ L+EQT
Sbjct: 193 KRFLPALKSEIRKEERKNDIDWEKTIILSRRCFHDDWAKIIDRLREQT 240

BLAST of Lag0006201 vs. ExPASy TrEMBL
Match: A0A0A0L427 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G572310 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.6e-17
Identity = 45/89 (50.56%), Postives = 59/89 (66.29%), Query Frame = 0

Query: 43  EEIILVWITDVIEDLLLSPNTHEFSRKTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKH 102
           EEI+ VW+ D +E++L +P + +F R+T C  GF WIQ+  NKRGSF+EI+KV   G K 
Sbjct: 281 EEILFVWLVDSLEEVLQTPKSDKFFRETFCNGGFSWIQETTNKRGSFLEISKVLKSGKKG 340

Query: 103 NLVIPVGADFNGRRDFARLLKDFLNGNEE 132
           N+V+P G D  G   F RLL DFLN   E
Sbjct: 341 NIVVPAGIDLKGWEQFRRLLLDFLNAEYE 369

BLAST of Lag0006201 vs. ExPASy TrEMBL
Match: A0A5A7TEP0 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00440 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 3.4e-12
Identity = 64/230 (27.83%), Postives = 110/230 (47.83%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           +E+K F    DK  +   + I E    + F+++     L W+    + LL +P T  F  
Sbjct: 13  VEKKDFVLSVDKRSRESNLLITEVGPYKSFSIAITLDSLEWLKTTFKALLNTPRTTRFFV 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNG 128
           +   V+  +W+QK+ N+RG   EI +V + G K  +++P G D  G   F+ +L      
Sbjct: 73  EKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFSDMLTCKKTS 132

Query: 129 NEEE---HHVVNAHKLKE---------------GTSYAKIVKYNLFVSERKMSKMERGNE 188
           +++E    H  N  K KE                 +YA++V  +   SE   SK +  + 
Sbjct: 133 DKKEISTRHYYNQDKGKEKIQKPYDSSTDSESPRKTYAEVVS-SFSSSESDYSKAKNTSS 192

Query: 189 RTTFGLKPYQWKVRK------IDWDEVIVVTKRDFHDDLGRILEILQEQT 215
              F L   + ++RK      IDW++ I++++R FHDD  +I++ L+EQT
Sbjct: 193 LKRF-LPAMKSEIRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQT 240

BLAST of Lag0006201 vs. ExPASy TrEMBL
Match: A0A5D3D494 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00820 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 2.2e-11
Identity = 62/228 (27.19%), Postives = 107/228 (46.93%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           +E+K      DK  +   + I E    + F+++     L W+    + LL +P T  F  
Sbjct: 13  VEKKDSVLSVDKRSRESNLLITEVGPYKSFSIAITPDSLEWLKTTFKALLNTPRTTRFFV 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNG 128
           +   V+  +W+QK+ N+RG   EI +V + G K  +++P G D  G   F  +L      
Sbjct: 73  EKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFNDMLTCKKTS 132

Query: 129 NEEE---HHVVNAHKLKE---------GTSYAKIVKYNLFVSERKMSKMERGNERTTFGL 188
           +++E    H  N  K KE           S +    Y   VS    S+ +    ++T  L
Sbjct: 133 DKKEISTRHFYNQDKGKEKIQKPYDSSTDSESPKKTYAEVVSSFSSSESDYSKAKSTSSL 192

Query: 189 KPY----QWKVRK------IDWDEVIVVTKRDFHDDLGRILEILQEQT 215
           K +    + ++RK      IDW++ I++++R FHDD  +I++ L+EQT
Sbjct: 193 KRFLPALKSEIRKEERKNDIDWEKTIILSRRCFHDDWAKIIDRLREQT 240

BLAST of Lag0006201 vs. ExPASy TrEMBL
Match: A0A5A7V5Z9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G001780 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 2.2e-11
Identity = 62/228 (27.19%), Postives = 107/228 (46.93%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           +E+K      DK  +   + I E    + F+++     L W+    + LL +P T  F  
Sbjct: 13  VEKKDSVLSVDKRSRESNLLITEVGPYKSFSIAITPDSLEWLKTTFKALLNTPRTTRFFV 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLLKDFLNG 128
           +   V+  +W+QK+ N+RG   EI +V + G K  +++P G D  G   F  +L      
Sbjct: 73  EKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFNDMLTCKKTS 132

Query: 129 NEEE---HHVVNAHKLKE---------GTSYAKIVKYNLFVSERKMSKMERGNERTTFGL 188
           +++E    H  N  K KE           S +    Y   VS    S+ +    ++T  L
Sbjct: 133 DKKEISTRHFYNQDKGKEKIQKPYDSSTDSESPRKTYAEVVSSFSSSESDYSKAKSTSSL 192

Query: 189 KPY----QWKVRK------IDWDEVIVVTKRDFHDDLGRILEILQEQT 215
           K +    + ++RK      IDW++ I++++R FHDD  +I++ L+EQT
Sbjct: 193 KRFLPALKSEIRKEERKNDIDWEKTIILSRRCFHDDWAKIIDRLREQT 240

BLAST of Lag0006201 vs. ExPASy TrEMBL
Match: A0A5A7TFK7 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00060 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.1e-10
Identity = 65/233 (27.90%), Postives = 100/233 (42.92%), Query Frame = 0

Query: 9   IERKFFSCCFDKNFKGRVVRICETHLKRRFTLSAEEIILVWITDVIEDLLLSPNTHEFSR 68
           IE+K F    D   +   + I E    + F+++     L W+      LL +P T  F  
Sbjct: 13  IEKKDFVLSVDNWSRDFKLLITEVGPYKSFSIAVTLESLEWLKSSFRTLLDTPRTTRFFL 72

Query: 69  KTDCVNGFIWIQKVLNKRGSFIEITKVSNVGGKHNLVIPVGADFNGRRDFARLL---KDF 128
           +       +W+QK  N++G   EI +V + G K  +++P GA+ +G   F  LL   KD 
Sbjct: 73  EKRFEECCLWVQKTYNRKGYIAEIYRVDDRGRKCCILVPEGAEKSGWAHFVSLLHGKKD- 132

Query: 129 LNGNEEEHHVVNAHKLKEG------------TSYAKIVKYNLFVSERKMSKMER----GN 188
              ++  +  +   K+++G             SYA+ V       E   S+ E     G 
Sbjct: 133 -PSSKTNYRTILNTKMRDGFSSSDSGEDTKRRSYAEAVIKGSSSDEESNSRTENIKKTGK 192

Query: 189 ERTTFGLKPYQWKVRKIDWDEVIVVTKRDFHDDLGRILEILQEQ-TIALHYKP 222
             TTF            DW+   V+T+R FHDD  RI+E L EQ    + YKP
Sbjct: 193 SNTTF----------SFDWERTAVLTRRYFHDDWERIVEKLNEQLDTTVRYKP 233

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031737554.17.8e-2747.01transcription factor Pur-alpha 1-like [Cucumis sativus][more]
KAG6601809.11.5e-1444.44hypothetical protein SDJN03_07042, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAA0041398.17.1e-1227.83hypothetical protein E6C27_scaffold206G00440 [Cucumis melo var. makuwa][more]
KAA0063662.14.6e-1127.19hypothetical protein E6C27_scaffold329G001780 [Cucumis melo var. makuwa][more]
TYK18363.14.6e-1127.19hypothetical protein E5676_scaffold456G00820 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L4274.6e-1750.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G572310 PE=4 SV=1[more]
A0A5A7TEP03.4e-1227.83DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3D4942.2e-1127.19Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7V5Z92.2e-1127.19Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5A7TFK71.1e-1027.90DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006201.1Lag0006201.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity