Lag0006175 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0006175 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 94.0 bits (232), Expect = 2.1e-15
Identity = 61/188 (32.45%), Postives = 92/188 (48.94%), Query Frame = 0

Query: 61  VDLFLKGYREHWLTLDYWDWLNQNLSSKELGVSMILMWSIWIFRNNNLNNPNII---QPD 120
           V+ F    R HW   D W+WL   LS +E+  SM++ W IW  RN ++     I   Q D
Sbjct: 2   VEHFYGNVRVHWTNKDSWNWLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLD 61

Query: 121 KN--KLINLIAGKLSKIKGRSHSYQINLKASRG-ENPSSQGI-WNPPPPLCWKLNVDASW 180
           ++    IN    K + I     S Q +    RG EN +  G+ W+ PP  CWKLN DASW
Sbjct: 62  RSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASW 121

Query: 181 NLLEDRGGLGWIVRDSGVGQLSGMLLFKHCPKEKNRVAHSIERGGVGLTRHTNFYNLGQR 240
           +   + GG+GWI+ D        ++L  +C   + +  +++E   + + R   F N+  R
Sbjct: 122 SEEREVGGIGWILCDC----RGEIVLAGNCKIREKKEINALEL--MTIIRGLQFINMQSR 181

Query: 241 LPSTLEGE 242
            P  LE +
Sbjct: 182 SPIYLESD 183

BLAST of Lag0006175 vs. NCBI nr
Match: XP_022148549.1 (uncharacterized protein LOC111017181 [Momordica charantia])

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-09
Identity = 47/131 (35.88%), Postives = 66/131 (50.38%), Query Frame = 0

Query: 75  LDYWDWLNQNLSSKELGVSMILMWSIWIFRNNNLNNPNIIQPDKNKLINLIAGKLSKIKG 134
           +DY+DW+ ++    +    ++L+WSIW +RN  +++  I QP   K   + A   SKI  
Sbjct: 1   MDYFDWIWRDSDQYKRQSGLVLLWSIWTYRNQIVHS-TIEQPCSQK---IRAFTESKIYE 60

Query: 135 RSHSYQINLKASRGENPSSQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRDSGVGQL 194
            + +   NL       P + G W PP    WKLNVDA+W      GGLGWIVRDS     
Sbjct: 61  FTTNLGGNLNMEDKNLPRTVG-WTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDS----- 120

Query: 195 SGMLLFKHCPK 206
            G  +   C K
Sbjct: 121 EGRFIMAECLK 121

BLAST of Lag0006175 vs. NCBI nr
Match: XP_019107216.1 (PREDICTED: uncharacterized protein LOC109136049 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 68.9 bits (167), Expect = 7.4e-08
Identity = 38/73 (52.05%), Postives = 46/73 (63.01%), Query Frame = 0

Query: 3   CISTVSCSMLVSGYPSKDFKPSRGIRQGDLLSPYLF-LCALKMPHWELIPPLHGEASIGV 62
           CI+TVS S+LV+G P + F P RGIRQGD LSPY+F LCA  +        L G  S+GV
Sbjct: 500 CITTVSFSVLVNGIPGETFVPQRGIRQGDPLSPYIFILCAEILARQLQHASLMGSRSLGV 559

Query: 63  DLFLKGYREHWLT 75
            L   G R H+LT
Sbjct: 560 TLGHSGVRIHFLT 572

BLAST of Lag0006175 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 67.4 bits (163), Expect = 2.2e-07
Identity = 36/101 (35.64%), Postives = 56/101 (55.45%), Query Frame = 0

Query: 93  SMILMWSIWIFRNNNL-----NNPNIIQPDKNKLINLIAGKLSKIKGRSHSYQINLKASR 152
           SMI+ W IW  RN ++     +    IQ   ++ I   AG+ + +KG+S +  ++L    
Sbjct: 12  SMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDLHLIRRI 71

Query: 153 GENPSSQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRD 189
           G+N  ++  W PP    WKLN DA+W    + GG+GWI+RD
Sbjct: 72  GDNTGAR--WKPPTSNSWKLNTDAAWRADTNTGGIGWILRD 110

BLAST of Lag0006175 vs. NCBI nr
Match: XP_023880292.1 (uncharacterized protein LOC111992663 [Quercus suber])

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-07
Identity = 51/196 (26.02%), Postives = 92/196 (46.94%), Query Frame = 0

Query: 1   MSCISTVSCSMLVSGYPSKDFKPSRGIRQGDLLSPYLFLCALKMPHWELIPPLHGEASIG 60
           M CIS+++ ++ ++G PS    P+RG+RQGD LSPYLFL  +     E +  L  +A+  
Sbjct: 110 MQCISSITYAIRINGKPSSHITPTRGLRQGDPLSPYLFLLCV-----EGLSTLIKKAT-- 169

Query: 61  VDLFLKGYRE-------HWLTLDYWDWLNQNLSSKELGVSMILMWSIWIFRNNNLNNPNI 120
            D  L+G  E        ++ + ++  +  +    E+   +++ W++W  RN   N    
Sbjct: 170 ADGLLEGISESRVQHFGSFMDMLWYVVMTAHWEHNEVEKLIVVAWALWSNRNECKNG--- 229

Query: 121 IQPDKNKLINLIAGKLSKIKGRSHSYQINLKASRG-ENPSSQGIWNPPPPLCWKLNVDAS 180
               K     L+ G L  +     +YQ  L  S   + P+    W P     +K+N+D +
Sbjct: 230 --GAKKSCQALLQGALEYL----DAYQACLMVSESPKQPAESMKWKPSSSNHYKVNMDGA 289

Query: 181 WNLLEDRGGLGWIVRD 189
               +   G+G +++D
Sbjct: 290 MFKEQRMAGVGILIKD 289

BLAST of Lag0006175 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.0e-15
Identity = 61/188 (32.45%), Postives = 92/188 (48.94%), Query Frame = 0

Query: 61  VDLFLKGYREHWLTLDYWDWLNQNLSSKELGVSMILMWSIWIFRNNNLNNPNII---QPD 120
           V+ F    R HW   D W+WL   LS +E+  SM++ W IW  RN ++     I   Q D
Sbjct: 2   VEHFYGNVRVHWTNKDSWNWLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLD 61

Query: 121 KN--KLINLIAGKLSKIKGRSHSYQINLKASRG-ENPSSQGI-WNPPPPLCWKLNVDASW 180
           ++    IN    K + I     S Q +    RG EN +  G+ W+ PP  CWKLN DASW
Sbjct: 62  RSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASW 121

Query: 181 NLLEDRGGLGWIVRDSGVGQLSGMLLFKHCPKEKNRVAHSIERGGVGLTRHTNFYNLGQR 240
           +   + GG+GWI+ D        ++L  +C   + +  +++E   + + R   F N+  R
Sbjct: 122 SEEREVGGIGWILCDC----RGEIVLAGNCKIREKKEINALEL--MTIIRGLQFINMQSR 181

Query: 241 LPSTLEGE 242
            P  LE +
Sbjct: 182 SPIYLESD 183

BLAST of Lag0006175 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 6.5e-10
Identity = 47/131 (35.88%), Postives = 66/131 (50.38%), Query Frame = 0

Query: 75  LDYWDWLNQNLSSKELGVSMILMWSIWIFRNNNLNNPNIIQPDKNKLINLIAGKLSKIKG 134
           +DY+DW+ ++    +    ++L+WSIW +RN  +++  I QP   K   + A   SKI  
Sbjct: 1   MDYFDWIWRDSDQYKRQSGLVLLWSIWTYRNQIVHS-TIEQPCSQK---IRAFTESKIYE 60

Query: 135 RSHSYQINLKASRGENPSSQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRDSGVGQL 194
            + +   NL       P + G W PP    WKLNVDA+W      GGLGWIVRDS     
Sbjct: 61  FTTNLGGNLNMEDKNLPRTVG-WTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDS----- 120

Query: 195 SGMLLFKHCPK 206
            G  +   C K
Sbjct: 121 EGRFIMAECLK 121

BLAST of Lag0006175 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.0e-07
Identity = 36/101 (35.64%), Postives = 56/101 (55.45%), Query Frame = 0

Query: 93  SMILMWSIWIFRNNNL-----NNPNIIQPDKNKLINLIAGKLSKIKGRSHSYQINLKASR 152
           SMI+ W IW  RN ++     +    IQ   ++ I   AG+ + +KG+S +  ++L    
Sbjct: 12  SMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDLHLIRRI 71

Query: 153 GENPSSQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRD 189
           G+N  ++  W PP    WKLN DA+W    + GG+GWI+RD
Sbjct: 72  GDNTGAR--WKPPTSNSWKLNTDAAWRADTNTGGIGWILRD 110

BLAST of Lag0006175 vs. ExPASy TrEMBL
Match: A0A803Q8J4 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-07
Identity = 40/117 (34.19%), Postives = 61/117 (52.14%), Query Frame = 0

Query: 76   DYWDWLNQNLSSKELGVSMILMWSIWIFRNNNLNNPNIIQPDKNKLINLIAGKLSK-IKG 135
            D+   +++N +  EL      MWSIW  RNN ++  +  QP       +I+ K    +  
Sbjct: 1567 DFLFQISENYTKSELETIFCTMWSIWSDRNNVIHGKSPQQP------TVISAKAQNFLNN 1626

Query: 136  RSHSYQINLKA--SRGENPSSQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRDS 190
               + Q++L A  S    PS    W+PPPP C KLNVDA+++   ++ G G I+RDS
Sbjct: 1627 YKSTQQLSLPAGLSPPATPSISKAWSPPPPTCLKLNVDAAFDEACNKIGFGAIIRDS 1677

BLAST of Lag0006175 vs. ExPASy TrEMBL
Match: A0A2N9GIZ4 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS27281 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 6.8e-07
Identity = 50/160 (31.25%), Postives = 74/160 (46.25%), Query Frame = 0

Query: 3   CISTVSCSMLVSGYPSKDFKPSRGIRQGDLLSPYLFLCALKMPHWELIPPLHGEASIGVD 62
           C++TVS S+L++G PSK F P+RG+RQGD LSPYLFL    +    L+   +        
Sbjct: 14  CVTTVSYSVLINGSPSKTFCPARGLRQGDPLSPYLFLLCANVLSCALLKQEN-------T 73

Query: 63  LFLKGYR--------EHWLTLDYWDWLNQNLSSKELGVSMILMW-------SIWIFRNNN 122
           L LKG +         H L  D +    ++  S    +   L W       SI + +   
Sbjct: 74  LHLKGVKIGRATQPLSHLLFADDFFLFFKHDKSSPNTIQSTLAWYCCLSGQSINLDKFEL 133

Query: 123 LNNPNIIQPDKNKLINLIAGKLSKIKGRSHSYQINLKASR 148
             +PN+ + DK+ L NL+  KL    G+       L+  R
Sbjct: 134 YCSPNMTEIDKSNLANLLGVKLVPSPGKYLGVNFKLRGGR 166

BLAST of Lag0006175 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.3e-06
Identity = 41/156 (26.28%), Postives = 69/156 (44.23%), Query Frame = 0

Query: 37  LFLCALKMPHWEL--IP-PLHGEASIGVDLFLKGYREHWLTLDYWDWLNQNLSSKELGVS 96
           LF C      W +  IP PL GE +  + + L           YW +   N + +    S
Sbjct: 309 LFKCTFARLTWAISSIPIPLGGEWADSIYVNL-----------YWVFNLGNGNPQWEKAS 368

Query: 97  MILMWSIW-IFRNNNLNNPNIIQPDKNKLINLIAGKLSKIKGRSHSYQINLKASRGENPS 156
            ++ W +W +++N N       + +  +++      L + + R+ +     K     N S
Sbjct: 369 QLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQ--VNRS 428

Query: 157 SQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRD 189
           S G W PPP    K N DA+WN   +R G+GW++R+
Sbjct: 429 SCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRN 451

BLAST of Lag0006175 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 9.6e-06
Identity = 53/232 (22.84%), Postives = 91/232 (39.22%), Query Frame = 0

Query: 37  LFLCALKMPHWELIP-PLHGEASIGVDLFLKGYREHWLTLDYWDWLNQNLSSKELG-VSM 96
           LF C      W + P P + E      L+   Y   W+       LN  +   +LG +  
Sbjct: 26  LFKCCFARLVWAISPIPAYPEGEWTDSLYANLY---WV-------LNLEVEIPKLGKIGN 85

Query: 97  ILMWSIW---------IFRNNNLNNPNIIQPDKNKLINLIAGKLSKIKGRSHSYQINLKA 156
           ++ W +W         +F+    + P +++            +  +++G++   Q+    
Sbjct: 86  LVPWLLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRR--ELEGKASGPQVE--- 145

Query: 157 SRGENPSSQGIWNPPPPLCWKLNVDASWNLLEDRGGLGWIVRDSGVGQLSGMLLFKHCPK 216
               N S Q  W  PP    K N DA+W L   R G+GWI+R+   G L   +  +  P+
Sbjct: 146 ---RNLSVQ--WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVL--WMGARALPR 205

Query: 217 EKNRVAHSIE--RGGVGLTRHTNFYNL-----GQRLPSTLEGEGRFWTSVYP 251
            KN +   +E  R  V      N+  +      Q L + L  +  FW ++ P
Sbjct: 206 TKNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDD-FWPTLQP 234