Lag0006162 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006162
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAA_kinase domain-containing protein
Locationchr6: 38402223 .. 38402825 (+)
RNA-Seq ExpressionLag0006162
SyntenyLag0006162
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGTGGCACATCTCAGCAACGTGACAAAGAGTCGTGCCATCCTCTTATTCGTCATCGCCACAAGGCGCTCAGTGAATGTCAGCCAGGTCATTCATCAGTCCATGCGCCACATCTTTTGGCGTAGCACGATAGTGGGGCTCGAGCATCCATCATTGATCACTGCCCTCTACCGAGCCGCTGGTGTCGTTTGGGACGCCCAGGAGGAGTTAGTCCACCCCGAAGCAGTTATAGACAAGAACTTCATCAGTCGATACCGAGGGTCTGGACAACAGGGTGCACAACCAGCTCTTCCCATTCCTGCACCGCCAGAGCATCCTGAGCCGTCTGCTGAGCCCAAGCATCAAGAGCAGGAGGTTCCACCTCCCACAGTCGAGGAGCAGTTGCAGCAGCTGCGCATGGAGTTCCAGAGTCACCGATTGGAGTTCCAGACATACCAGCTTGAGTTTCAGAGTCACCGGTTGGAGCTCCAAAGTCAGCAGTGCGATTACCAGAGAGAGCGGCGTATGGATCGACGTCACTTCCTCTACACCACGAGCATGTATGCCCACTCTTATCAATGTAAGGTGGCTTCCAGTACGGATTTTACCCCTGACCTCTGA

mRNA sequence

ATGTTTGTGGCACATCTCAGCAACGTGACAAAGAGTCGTGCCATCCTCTTATTCGTCATCGCCACAAGGCGCTCAGTGAATGTCAGCCAGGTCATTCATCAGTCCATGCGCCACATCTTTTGGCGTAGCACGATAGTGGGGCTCGAGCATCCATCATTGATCACTGCCCTCTACCGAGCCGCTGGTGTCGTTTGGGACGCCCAGGAGGAGTTAGTCCACCCCGAAGCAGTTATAGACAAGAACTTCATCAGTCGATACCGAGGGTCTGGACAACAGGGTGCACAACCAGCTCTTCCCATTCCTGCACCGCCAGAGCATCCTGAGCCGTCTGCTGAGCCCAAGCATCAAGAGCAGGAGGTTCCACCTCCCACAGTCGAGGAGCAGTTGCAGCAGCTGCGCATGGAGTTCCAGAGTCACCGATTGGAGTTCCAGACATACCAGCTTGAGTTTCAGAGTCACCGGTTGGAGCTCCAAAGTCAGCAGTGCGATTACCAGAGAGAGCGGCGTATGGATCGACGTCACTTCCTCTACACCACGAGCATGTATGCCCACTCTTATCAATGTAAGGTGGCTTCCAGTACGGATTTTACCCCTGACCTCTGA

Coding sequence (CDS)

ATGTTTGTGGCACATCTCAGCAACGTGACAAAGAGTCGTGCCATCCTCTTATTCGTCATCGCCACAAGGCGCTCAGTGAATGTCAGCCAGGTCATTCATCAGTCCATGCGCCACATCTTTTGGCGTAGCACGATAGTGGGGCTCGAGCATCCATCATTGATCACTGCCCTCTACCGAGCCGCTGGTGTCGTTTGGGACGCCCAGGAGGAGTTAGTCCACCCCGAAGCAGTTATAGACAAGAACTTCATCAGTCGATACCGAGGGTCTGGACAACAGGGTGCACAACCAGCTCTTCCCATTCCTGCACCGCCAGAGCATCCTGAGCCGTCTGCTGAGCCCAAGCATCAAGAGCAGGAGGTTCCACCTCCCACAGTCGAGGAGCAGTTGCAGCAGCTGCGCATGGAGTTCCAGAGTCACCGATTGGAGTTCCAGACATACCAGCTTGAGTTTCAGAGTCACCGGTTGGAGCTCCAAAGTCAGCAGTGCGATTACCAGAGAGAGCGGCGTATGGATCGACGTCACTTCCTCTACACCACGAGCATGTATGCCCACTCTTATCAATGTAAGGTGGCTTCCAGTACGGATTTTACCCCTGACCTCTGA

Protein sequence

MFVAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAGVVWDAQEELVHPEAVIDKNFISRYRGSGQQGAQPALPIPAPPEHPEPSAEPKHQEQEVPPPTVEEQLQQLRMEFQSHRLEFQTYQLEFQSHRLELQSQQCDYQRERRMDRRHFLYTTSMYAHSYQCKVASSTDFTPDL
Homology
BLAST of Lag0006162 vs. NCBI nr
Match: KAA0033353.1 (putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa] >TYJ96637.1 putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-13
Identity = 67/162 (41.36%), Postives = 89/162 (54.94%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           VAH S+VTK RAILL+ IAT+RSV+V +VIH+S+ +I       GL H SLITAL R  G
Sbjct: 181 VAHTSSVTKERAILLYAIATKRSVDVGKVIHKSLCNIRKSGMTGGLGHSSLITALCRNEG 240

Query: 63  VVWDAQEELVHPEAVIDKNFIS-------RYRGSGQ----QGAQPALPIPAPPEHPEPS- 122
           VVW+ +EELV P+ ++DKNFI           G+G      GA      P    H EPS 
Sbjct: 241 VVWNEKEELVDPKPIMDKNFIMGIPGWSFETMGAGHCDETAGASHCNKTPDAGHHDEPSD 300

Query: 123 ---AEPKHQ----------EQEVPPPTVEEQLQQLRMEFQSH 140
              AEP  +           Q   P +++EQ+Q+L    +S+
Sbjct: 301 QDEAEPIREVRQTLTIDLPRQTQRPLSLDEQIQRLERRVRSY 342

BLAST of Lag0006162 vs. NCBI nr
Match: XP_008458668.1 (PREDICTED: uncharacterized protein LOC103497996 [Cucumis melo])

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-13
Identity = 67/162 (41.36%), Postives = 89/162 (54.94%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           VAH S+VTK RAILL+ IAT+RSV+V +VIH+S+ +I       GL H SLITAL R  G
Sbjct: 119 VAHTSSVTKERAILLYAIATKRSVDVGKVIHKSLCNIRKSGMTGGLGHSSLITALCRNEG 178

Query: 63  VVWDAQEELVHPEAVIDKNFIS-------RYRGSGQ----QGAQPALPIPAPPEHPEPS- 122
           VVW+ +EELV P+ ++DKNFI           G+G      GA      P    H EPS 
Sbjct: 179 VVWNEKEELVDPKPIMDKNFIMGIPGWSFETMGAGHCDETAGASHCNKTPDAGHHDEPSD 238

Query: 123 ---AEPKHQ----------EQEVPPPTVEEQLQQLRMEFQSH 140
              AEP  +           Q   P +++EQ+Q+L    +S+
Sbjct: 239 QDEAEPIREVRQTLTIDLPRQTQRPLSLDEQIQRLERRVRSY 280

BLAST of Lag0006162 vs. NCBI nr
Match: KGN46897.1 (hypothetical protein Csa_020731 [Cucumis sativus])

HSP 1 Score: 74.7 bits (182), Expect = 1.0e-09
Identity = 66/195 (33.85%), Postives = 100/195 (51.28%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           VAH S+VTK RAILL+ IAT+RSV+V +VI +S+ +I       GL H SLITAL R  G
Sbjct: 181 VAHTSSVTKERAILLYAIATKRSVDVGKVIQKSLCNIRKSGMTGGLGHSSLITALCRNEG 240

Query: 63  VVWDAQEELVHPEAVIDKNFISRYRG-SGQQGAQPALPIPAPPEHPEPSAEPKHQEQEVP 122
           VVW+ +EELV P+ ++DK+FI    G S +          A   H   + +  H ++   
Sbjct: 241 VVWNEKEELVDPKPIMDKSFIMEIPGWSFEPMGAGHCDETAGTSHCNKTTDAGHNDE--- 300

Query: 123 PPTVEEQLQQLRMEFQSHRLEF-----QTYQLEFQSHRLELQSQQCDYQRERRMDRRHFL 182
            P+ +++ + +R   Q+  ++      +   L+ Q  RLE + +    + E R D     
Sbjct: 301 -PSDQDEAEPIREVRQTLTIDLPRQTQRPLSLDEQIRRLERRVRSYHRRSEERFD----- 359

Query: 183 YTTSMYAHSYQCKVA 192
                  H Y+C  A
Sbjct: 361 -------HLYKCLFA 359

BLAST of Lag0006162 vs. NCBI nr
Match: KAA0047231.1 (uncharacterized protein E6C27_scaffold908G00220 [Cucumis melo var. makuwa])

HSP 1 Score: 73.9 bits (180), Expect = 1.7e-09
Identity = 36/88 (40.91%), Postives = 58/88 (65.91%), Query Frame = 0

Query: 1  MFVAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRA 60
          M V   S+VT  RA+LLFV+A+  S++V ++I  S++  +   T  GL HPSLIT LYR 
Sbjct: 1  MSVTRFSDVTNDRALLLFVLASGLSIDVRKIIFHSIKLTYRTPTTYGLRHPSLITELYRQ 60

Query: 61 AGVVWDAQEELVHPEAVIDKNFISRYRG 89
          AGV +   +E++   A ++++F+S ++G
Sbjct: 61 AGVEFGIDKEMIFSRAFLNRSFVSAFQG 88

BLAST of Lag0006162 vs. NCBI nr
Match: TYK21755.1 (uncharacterized protein E5676_scaffold1721G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 72.4 bits (176), Expect = 5.0e-09
Identity = 36/88 (40.91%), Postives = 57/88 (64.77%), Query Frame = 0

Query: 1  MFVAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRA 60
          M V   SNVT  RA+LLFV+A+  S++V ++I  S++  +   T  GL HPSLIT L R 
Sbjct: 1  MSVTQFSNVTNDRALLLFVLASGLSIDVRKIIFHSIKLTYRTPTTYGLRHPSLITELCRQ 60

Query: 61 AGVVWDAQEELVHPEAVIDKNFISRYRG 89
          AGV +   +E++   A ++++F+S ++G
Sbjct: 61 AGVEFGIDKEMIFSRAFLNRSFVSAFQG 88

BLAST of Lag0006162 vs. ExPASy TrEMBL
Match: A0A5D3BBY3 (Putative S-locus lectin protein kinase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00020 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.2e-13
Identity = 67/162 (41.36%), Postives = 89/162 (54.94%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           VAH S+VTK RAILL+ IAT+RSV+V +VIH+S+ +I       GL H SLITAL R  G
Sbjct: 181 VAHTSSVTKERAILLYAIATKRSVDVGKVIHKSLCNIRKSGMTGGLGHSSLITALCRNEG 240

Query: 63  VVWDAQEELVHPEAVIDKNFIS-------RYRGSGQ----QGAQPALPIPAPPEHPEPS- 122
           VVW+ +EELV P+ ++DKNFI           G+G      GA      P    H EPS 
Sbjct: 241 VVWNEKEELVDPKPIMDKNFIMGIPGWSFETMGAGHCDETAGASHCNKTPDAGHHDEPSD 300

Query: 123 ---AEPKHQ----------EQEVPPPTVEEQLQQLRMEFQSH 140
              AEP  +           Q   P +++EQ+Q+L    +S+
Sbjct: 301 QDEAEPIREVRQTLTIDLPRQTQRPLSLDEQIQRLERRVRSY 342

BLAST of Lag0006162 vs. ExPASy TrEMBL
Match: A0A1S3C7Y0 (uncharacterized protein LOC103497996 OS=Cucumis melo OX=3656 GN=LOC103497996 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.2e-13
Identity = 67/162 (41.36%), Postives = 89/162 (54.94%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           VAH S+VTK RAILL+ IAT+RSV+V +VIH+S+ +I       GL H SLITAL R  G
Sbjct: 119 VAHTSSVTKERAILLYAIATKRSVDVGKVIHKSLCNIRKSGMTGGLGHSSLITALCRNEG 178

Query: 63  VVWDAQEELVHPEAVIDKNFIS-------RYRGSGQ----QGAQPALPIPAPPEHPEPS- 122
           VVW+ +EELV P+ ++DKNFI           G+G      GA      P    H EPS 
Sbjct: 179 VVWNEKEELVDPKPIMDKNFIMGIPGWSFETMGAGHCDETAGASHCNKTPDAGHHDEPSD 238

Query: 123 ---AEPKHQ----------EQEVPPPTVEEQLQQLRMEFQSH 140
              AEP  +           Q   P +++EQ+Q+L    +S+
Sbjct: 239 QDEAEPIREVRQTLTIDLPRQTQRPLSLDEQIQRLERRVRSY 280

BLAST of Lag0006162 vs. ExPASy TrEMBL
Match: A0A0A0KNI1 (AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G468460 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.1e-13
Identity = 46/86 (53.49%), Postives = 61/86 (70.93%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           +AH S+VTK RAILL+ IAT+RSV+V +VI +S+ +I       GL H SLITAL R  G
Sbjct: 152 MAHTSSVTKERAILLYAIATKRSVDVGKVIQKSLCNIRKSGMTGGLGHSSLITALCRNEG 211

Query: 63  VVWDAQEELVHPEAVIDKNFISRYRG 89
           VVW+ +EELV P+ ++DK+FI    G
Sbjct: 212 VVWNEKEELVDPKPIMDKSFIMEIPG 237

BLAST of Lag0006162 vs. ExPASy TrEMBL
Match: A0A0A0KER1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149380 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.9e-10
Identity = 66/195 (33.85%), Postives = 100/195 (51.28%), Query Frame = 0

Query: 3   VAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRAAG 62
           VAH S+VTK RAILL+ IAT+RSV+V +VI +S+ +I       GL H SLITAL R  G
Sbjct: 181 VAHTSSVTKERAILLYAIATKRSVDVGKVIQKSLCNIRKSGMTGGLGHSSLITALCRNEG 240

Query: 63  VVWDAQEELVHPEAVIDKNFISRYRG-SGQQGAQPALPIPAPPEHPEPSAEPKHQEQEVP 122
           VVW+ +EELV P+ ++DK+FI    G S +          A   H   + +  H ++   
Sbjct: 241 VVWNEKEELVDPKPIMDKSFIMEIPGWSFEPMGAGHCDETAGTSHCNKTTDAGHNDE--- 300

Query: 123 PPTVEEQLQQLRMEFQSHRLEF-----QTYQLEFQSHRLELQSQQCDYQRERRMDRRHFL 182
            P+ +++ + +R   Q+  ++      +   L+ Q  RLE + +    + E R D     
Sbjct: 301 -PSDQDEAEPIREVRQTLTIDLPRQTQRPLSLDEQIRRLERRVRSYHRRSEERFD----- 359

Query: 183 YTTSMYAHSYQCKVA 192
                  H Y+C  A
Sbjct: 361 -------HLYKCLFA 359

BLAST of Lag0006162 vs. ExPASy TrEMBL
Match: A0A5A7U0V9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00220 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 8.3e-10
Identity = 36/88 (40.91%), Postives = 58/88 (65.91%), Query Frame = 0

Query: 1  MFVAHLSNVTKSRAILLFVIATRRSVNVSQVIHQSMRHIFWRSTIVGLEHPSLITALYRA 60
          M V   S+VT  RA+LLFV+A+  S++V ++I  S++  +   T  GL HPSLIT LYR 
Sbjct: 1  MSVTRFSDVTNDRALLLFVLASGLSIDVRKIIFHSIKLTYRTPTTYGLRHPSLITELYRQ 60

Query: 61 AGVVWDAQEELVHPEAVIDKNFISRYRG 89
          AGV +   +E++   A ++++F+S ++G
Sbjct: 61 AGVEFGIDKEMIFSRAFLNRSFVSAFQG 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0033353.12.6e-1341.36putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa]... [more]
XP_008458668.12.6e-1341.36PREDICTED: uncharacterized protein LOC103497996 [Cucumis melo][more]
KGN46897.11.0e-0933.85hypothetical protein Csa_020731 [Cucumis sativus][more]
KAA0047231.11.7e-0940.91uncharacterized protein E6C27_scaffold908G00220 [Cucumis melo var. makuwa][more]
TYK21755.15.0e-0940.91uncharacterized protein E5676_scaffold1721G00020 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BBY31.2e-1341.36Putative S-locus lectin protein kinase family protein OS=Cucumis melo var. makuw... [more]
A0A1S3C7Y01.2e-1341.36uncharacterized protein LOC103497996 OS=Cucumis melo OX=3656 GN=LOC103497996 PE=... [more]
A0A0A0KNI12.1e-1353.49AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G468460 P... [more]
A0A0A0KER14.9e-1033.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149380 PE=4 SV=1[more]
A0A5A7U0V98.3e-1040.91Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..149
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 88..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006162.1Lag0006162.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006561 proline biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004349 glutamate 5-kinase activity