Lag0005628 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005628
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease H
Location	chr6: 24330752 .. 24331402 (+)
RNA-Seq Expression	Lag0005628
Synteny	Lag0005628

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACGAATCCTGAAAGTATATGAAGTAGCCACTGGGCAAGTCTTTAATTTTGATAAATCGGAGATAGCTTTTGGGACAGGAGTGACAAATGAAGTAAAAGACTTTTTAGCCAACATTTTAAAAGTCAAGATAGTCCAAGACCATGGAAAGTATCTTGGATGTTCGTCTTCCCTATCTAGAAACAAGAAAGCTTCTTTGAATTACATAACATCTAAAGTTCAGAAGGTGCTGCAGGGTTGGAAAGCAAAATTATTTTCAACGACTGGAAAGGAAGTGTTGATAAAGGTTGTAGCTCAGGCAATCCCCACTTATACTCTCTCATGTTTCAAACTTCCTCAGGGGTGCTGCCATGACCTGAACAGGCTGATGGCTAATTTTTGGTGGGGTAGTTCTGAACAGGGGAGGAAAATTCATTGGCTGAAATGGGAAACAATGACAAAACTAAAGGATAGAGGAGGGATGAGTTTTAGAGATATTAGTCTGTTCAACCAGGCCTTGTTGGCCAAACATACCTGGAGACTATTACATAGACCACAATCTCTAATGGCCCAAATCCTGAAAGCCATATATTATCCTCAGGAAGATTTCCTAAAAGCAACTTTGGGAAGCAGACCATCTTATGTTTGGAGGGGGATCATTTGGGGATGA

mRNA sequence

ATGCAACGAATCCTGAAAGTATATGAAGTAGCCACTGGGCAAGTCTTTAATTTTGATAAATCGGAGATAGCTTTTGGGACAGGAGTGACAAATGAAGTAAAAGACTTTTTAGCCAACATTTTAAAAGTCAAGATAGTCCAAGACCATGGAAAGTATCTTGGATGTTCGTCTTCCCTATCTAGAAACAAGAAAGCTTCTTTGAATTACATAACATCTAAAGTTCAGAAGGTGCTGCAGGGTTGGAAAGCAAAATTATTTTCAACGACTGGAAAGGAAGTGTTGATAAAGGTTGTAGCTCAGGCAATCCCCACTTATACTCTCTCATGTTTCAAACTTCCTCAGGGGTGCTGCCATGACCTGAACAGGCTGATGGCTAATTTTTGGTGGGGTAGTTCTGAACAGGGGAGGAAAATTCATTGGCTGAAATGGGAAACAATGACAAAACTAAAGGATAGAGGAGGGATGAGTTTTAGAGATATTAGTCTGTTCAACCAGGCCTTGTTGGCCAAACATACCTGGAGACTATTACATAGACCACAATCTCTAATGGCCCAAATCCTGAAAGCCATATATTATCCTCAGGAAGATTTCCTAAAAGCAACTTTGGGAAGCAGACCATCTTATGTTTGGAGGGGGATCATTTGGGGATGA

Coding sequence (CDS)

ATGCAACGAATCCTGAAAGTATATGAAGTAGCCACTGGGCAAGTCTTTAATTTTGATAAATCGGAGATAGCTTTTGGGACAGGAGTGACAAATGAAGTAAAAGACTTTTTAGCCAACATTTTAAAAGTCAAGATAGTCCAAGACCATGGAAAGTATCTTGGATGTTCGTCTTCCCTATCTAGAAACAAGAAAGCTTCTTTGAATTACATAACATCTAAAGTTCAGAAGGTGCTGCAGGGTTGGAAAGCAAAATTATTTTCAACGACTGGAAAGGAAGTGTTGATAAAGGTTGTAGCTCAGGCAATCCCCACTTATACTCTCTCATGTTTCAAACTTCCTCAGGGGTGCTGCCATGACCTGAACAGGCTGATGGCTAATTTTTGGTGGGGTAGTTCTGAACAGGGGAGGAAAATTCATTGGCTGAAATGGGAAACAATGACAAAACTAAAGGATAGAGGAGGGATGAGTTTTAGAGATATTAGTCTGTTCAACCAGGCCTTGTTGGCCAAACATACCTGGAGACTATTACATAGACCACAATCTCTAATGGCCCAAATCCTGAAAGCCATATATTATCCTCAGGAAGATTTCCTAAAAGCAACTTTGGGAAGCAGACCATCTTATGTTTGGAGGGGGATCATTTGGGGATGA

Protein sequence

MQRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLSRNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDLNRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQSLMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG

Homology

[Close]

BLAST of Lag0005628 vs. NCBI nr
Match: PNY02790.1 (ribonuclease H, partial [Trifolium pratense])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 108/214 (50.47%), Postives = 150/214 (70.09%), Query Frame = 0


Query: 1   MQRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLS 60
           +Q I+++Y+ A+GQ+ NF KSE+ F  GV+N+++  ++NIL +KI+    KYLG   +L 
Sbjct: 342 IQNIIQIYQNASGQLVNFSKSEMVFSKGVSNQIQRDISNILPMKILNQFSKYLGMPVALG 401

Query: 61  RNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDL 120
           R+KK   N+I  K+ K L+GWK K  S  G+  LIK VAQAIPTY +S F LP+G C  L
Sbjct: 402 RSKKQIFNFIQEKIWKKLKGWKEKHLSFAGRGTLIKAVAQAIPTYLMSSFLLPKGVCDQL 461

Query: 121 NRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQ 180
            +++ NFWWGSS   RKIHW+KW T+ K K  GG+ FRD+  FN+ALLAK  WRL+ +P 
Sbjct: 462 EKMICNFWWGSSTDHRKIHWIKWSTVCKHKMEGGIGFRDLRAFNEALLAKQGWRLITQPD 521

Query: 181 SLMAQILKAIYYPQEDFLKATLGSRPSYVWRGII 215
           SL+A+ILKA YYP+ +FLKA+   + SY W+ I+
Sbjct: 522 SLVAKILKAKYYPKGEFLKASHKQQMSYTWKSIL 555

[Close]

BLAST of Lag0005628 vs. NCBI nr
Match: PNX98065.1 (ribonuclease H [Trifolium pratense])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 108/214 (50.47%), Postives = 150/214 (70.09%), Query Frame = 0


Query: 1   MQRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLS 60
           +Q I+++Y+ A+GQ+ NF KSE+ F  GV+N+++  ++NIL +KI+    KYLG   +L 
Sbjct: 342 IQNIIQIYQNASGQLVNFSKSEMVFSKGVSNQIQRDISNILPMKILNQFSKYLGMPVALG 401

Query: 61  RNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDL 120
           R+KK   N+I  K+ K L+GWK K  S  G+  LIK VAQAIPTY +S F LP+G C  L
Sbjct: 402 RSKKQIFNFIQEKIWKKLKGWKEKHLSFAGRGTLIKAVAQAIPTYLMSSFLLPKGVCDQL 461

Query: 121 NRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQ 180
            +++ NFWWGSS   RKIHW+KW T+ K K  GG+ FRD+  FN+ALLAK  WRL+ +P 
Sbjct: 462 EKMICNFWWGSSTDHRKIHWIKWSTVCKHKMEGGIGFRDLRAFNEALLAKQGWRLITQPD 521

Query: 181 SLMAQILKAIYYPQEDFLKATLGSRPSYVWRGII 215
           SL+A+ILKA YYP+ +FLKA+   + SY W+ I+
Sbjct: 522 SLVAKILKAKYYPKGEFLKASHKQQMSYTWKSIL 555

[Close]

BLAST of Lag0005628 vs. NCBI nr
Match: PNY02482.1 (ribonuclease H, partial [Trifolium pratense])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 108/214 (50.47%), Postives = 150/214 (70.09%), Query Frame = 0


Query: 1   MQRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLS 60
           +Q I+++Y+ A+GQ+ NF KSE+ F  GV+N+++  ++NIL +KI+    KYLG   +L 
Sbjct: 496 IQNIIQIYQNASGQLVNFSKSEMVFSKGVSNQIQRDISNILPMKILNQFSKYLGMPVALG 555

Query: 61  RNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDL 120
           R+KK   N+I  K+ K L+GWK K  S  G+  LIK VAQAIPTY +S F LP+G C  L
Sbjct: 556 RSKKQIFNFIQEKIWKKLKGWKEKHLSFAGRGTLIKAVAQAIPTYLMSSFLLPKGVCDQL 615

Query: 121 NRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQ 180
            +++ NFWWGSS   RKIHW+KW T+ K K  GG+ FRD+  FN+ALLAK  WRL+ +P 
Sbjct: 616 EKMICNFWWGSSTDHRKIHWIKWSTVCKHKMEGGIGFRDLRAFNEALLAKQGWRLITQPD 675

Query: 181 SLMAQILKAIYYPQEDFLKATLGSRPSYVWRGII 215
           SL+A+ILKA YYP+ +FLKA+   + SY W+ I+
Sbjct: 676 SLVAKILKAKYYPKGEFLKASHKQQMSYTWKSIL 709

[Close]

BLAST of Lag0005628 vs. NCBI nr
Match: VVA38592.1 (PREDICTED: reverse mRNAase, partial [Prunus dulcis])

HSP 1 Score: 233.0 bits (593), Expect = 2.4e-57
Identity = 107/213 (50.23%), Postives = 142/213 (66.67%), Query Frame = 0


Query: 1   MQRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLS 60
           ++ I +VYE A+GQ  N  KS +AF T +  + +  LA++L V  V  H  YLG    L 
Sbjct: 315 LKHIFEVYERASGQQINCQKSCVAFSTNIHMDTQSRLASVLGVPRVDSHATYLGLPMMLG 374

Query: 61  RNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDL 120
           RNK     Y+  +V K LQGW+ +  S  GKEVL+KVVAQ+IP Y +SCF LPQG CH++
Sbjct: 375 RNKTFCFRYLKERVWKKLQGWREQTLSIAGKEVLLKVVAQSIPLYVMSCFLLPQGLCHEI 434

Query: 121 NRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQ 180
            ++MA FWWG   + RKIHW++WE + K K  GGM FR +  FN A+LAK  WRL+H P 
Sbjct: 435 EQMMARFWWGQQGENRKIHWMRWERLCKAKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPH 494

Query: 181 SLMAQILKAIYYPQEDFLKATLGSRPSYVWRGI 214
           SL +++LKA Y+PQ +F +ATLGSRPS VW+ I
Sbjct: 495 SLASRLLKAKYFPQTNFWEATLGSRPSCVWKSI 527

[Close]

BLAST of Lag0005628 vs. NCBI nr
Match: PNX92710.1 (ribonuclease H [Trifolium pratense])

HSP 1 Score: 230.3 bits (586), Expect = 1.5e-56
Identity = 104/211 (49.29%), Postives = 141/211 (66.82%), Query Frame = 0


Query: 4   ILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLSRNK 63
           IL+VY+ A+GQV N DKSE +F   V NE K+ + N++  K V+   +YLG      R+K
Sbjct: 677 ILQVYQQASGQVVNLDKSEASFSRNVQNEDKNMICNMMGAKAVEAQSRYLGFPIPFGRSK 736

Query: 64  KASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDLNRL 123
           K   + +  +V K ++GWK +  S  GKE LIK VAQAIP Y LSC+K+P GCC D+N +
Sbjct: 737 KVVFSVVMDRVWKKVKGWKERFLSRAGKETLIKAVAQAIPNYILSCYKMPVGCCKDINSM 796

Query: 124 MANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQSLM 183
           +A FWWGS+E+ RKIHW+ WE +++ K+ GGM FR I  FN+ALL KH WRL     SL+
Sbjct: 797 LAKFWWGSNEEKRKIHWMSWERLSRAKNDGGMGFRGIEEFNKALLGKHCWRLATGGSSLL 856

Query: 184 AQILKAIYYPQEDFLKATLGSRPSYVWRGII 215
            +I K+ YYP+ DF+ A  G +PSY WR I+
Sbjct: 857 EKIFKSRYYPKSDFMCANEGYQPSYAWRSIL 887

[Close]

BLAST of Lag0005628 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.3e-29
Identity = 53/117 (45.30%), Postives = 80/117 (68.38%), Query Frame = 0


Query: 101 AIPTYTLSCFKLPQGCCHDLNRLMANFWWGSSEQGRKIHWLKWETMTKLK-DRGGMSFRD 160
           A+P Y +SCF+L +  C  L   M  FWW S E  RKI W+ W+ + K K D GG+ FRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 161 ISLFNQALLAKHTWRLLHRPQSLMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG 217
           +  FNQALLAK ++R++H+P +L++++L++ Y+P    ++ ++G+RPSY WR II G
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHG 118

[Close]

BLAST of Lag0005628 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 50/158 (31.65%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 61  RNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDL 120
           R  K +   I  +V   + GW+ K  S  G+  L K V  ++P +++S   LPQ   + L
Sbjct: 7   RINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRL 66

Query: 121 NRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQ 180
           ++L   F WGS+ + +K H +KW  +   K  GG+  R     N+AL++K  WRLL    
Sbjct: 67  DQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKN 126

Query: 181 SLMAQILKAIYYPQEDFLKATLGSRPSY--VWRGIIWG 217
           SL   +L+  Y+  E      L  + S+   WR I  G
Sbjct: 127 SLWTLVLQKKYHVGEIRDSRWLIPKGSWSSTWRSIAIG 164

[Close]

BLAST of Lag0005628 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.1e-60
Identity = 111/215 (51.63%), Postives = 152/215 (70.70%), Query Frame = 0


Query: 2   QRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLSR 61
           Q++L  Y  A+GQ+ N+ KSE  FG  VT EV+  LA IL V+ V++HGKYLG  S + R
Sbjct: 47  QQLLSKYTAASGQLVNYHKSEAYFGRNVTAEVRSLLAVILGVREVENHGKYLGLPSFVGR 106

Query: 62  NKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDLN 121
           NKK  L+ I +KV   ++GWK  +FS  GKEVLIKV+ QAIPTYT+SCFKLP+     L+
Sbjct: 107 NKKEFLDEIKNKVWAKMKGWKTSMFSVAGKEVLIKVIVQAIPTYTMSCFKLPKKTISSLH 166

Query: 122 RLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQS 181
           R+ + FWWGS ++ +KIHW KW  + + KD+GG+ FRD+ +FNQALLAK  WR L  PQ 
Sbjct: 167 RMASRFWWGSLDKEKKIHWCKWRYLCRPKDKGGLGFRDLGMFNQALLAKQIWRCLRHPQL 226

Query: 182 LMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG 217
           L +++LKA Y+P++  L+A  G+  S+VWR ++WG
Sbjct: 227 LCSRVLKASYFPRKGVLEAGCGANASFVWRSLVWG 261

[Close]

BLAST of Lag0005628 vs. ExPASy TrEMBL
Match: A0A803PM68 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 9.4e-60
Identity = 107/215 (49.77%), Postives = 149/215 (69.30%), Query Frame = 0


Query: 2   QRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLSR 61
           Q +L+ Y +A+GQV NF KSE+ FG  V++ +K  LA ++ VK+V ++GKYLG  S + R
Sbjct: 40  QELLEKYSIASGQVVNFHKSEMCFGRTVSDPLKSQLAALMGVKVVDNYGKYLGLPSFVGR 99

Query: 62  NKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDLN 121
            KK    +I +KV   L+GWK   FS  GKE+LIK + QAIPTYT+SCF+LP+   + ++
Sbjct: 100 TKKQHFEFIKTKVWNKLKGWKGSFFSAAGKEILIKAIVQAIPTYTMSCFRLPKKTINSIH 159

Query: 122 RLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQS 181
            + A FWWGSSE+  KIHW KW T+ K K++GG+ FRD+ LFNQALLAK  WR +  P S
Sbjct: 160 SMAARFWWGSSEKDAKIHWCKWSTLCKHKEQGGLGFRDLGLFNQALLAKQIWRCIRYPNS 219

Query: 182 LMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG 217
           L +++LKA Y+P    L+A  G+  S+VWR ++WG
Sbjct: 220 LCSKVLKASYFPNVGVLEAKSGNHASFVWRSLVWG 254

[Close]

BLAST of Lag0005628 vs. ExPASy TrEMBL
Match: A0A803PWX1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.2e-59
Identity = 109/215 (50.70%), Postives = 148/215 (68.84%), Query Frame = 0


Query: 2    QRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLSR 61
            + +L+ Y  A+GQ+ NF KSE+ FG  VT  V+  LANI+ VK+V ++GKYLG  S + R
Sbjct: 1248 KELLEKYSKASGQLVNFHKSEMCFGRKVTLPVRTHLANIMGVKVVDNYGKYLGLPSFVGR 1307

Query: 62   NKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDLN 121
             KK    +I +KV   L+GWK   FS  GKEVLIK V QAIPTYT+SCF+LP+   + ++
Sbjct: 1308 TKKQHFEFIKNKVWNKLKGWKGSFFSAAGKEVLIKAVIQAIPTYTMSCFRLPKKTINSIH 1367

Query: 122  RLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQS 181
             + A FWWGSSE+  KIHW KW+ + K K++GG+ FRD+ LFNQALLAK  WR +  P S
Sbjct: 1368 SMAARFWWGSSEKDSKIHWCKWDVLCKHKEQGGLGFRDLGLFNQALLAKQVWRCIRFPNS 1427

Query: 182  LMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG 217
            L +++LKA YYP    ++A  G+  S+VWR ++WG
Sbjct: 1428 LCSRVLKASYYPNGGVIEAKSGNHASFVWRSLVWG 1462

[Close]

BLAST of Lag0005628 vs. ExPASy TrEMBL
Match: A0A803Q1K6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.6e-59
Identity = 109/215 (50.70%), Postives = 151/215 (70.23%), Query Frame = 0


Query: 2    QRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLSR 61
            Q++L  Y  A+GQ+ N+ KSE  FG  VT EV+  LA IL V+ V++HGKYLG  S + R
Sbjct: 1002 QQLLSKYTAASGQLVNYHKSEACFGRNVTAEVRSLLAGILGVREVENHGKYLGLPSFVGR 1061

Query: 62   NKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDLN 121
            NK+  L+ I +KV   ++GWK  +FS  GKEVLIK + QAIPTYT+SCFKLP+     L+
Sbjct: 1062 NKREFLDEIKNKVWAKMKGWKTSMFSVAGKEVLIKAIVQAIPTYTMSCFKLPKKTISSLH 1121

Query: 122  RLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQS 181
            R+ + FWWGSS++ +KIHW KW  + + KD+GG+ FRD+ +FNQALLAK  WR L  PQ 
Sbjct: 1122 RMASRFWWGSSDKEKKIHWCKWRYLCRPKDKGGLGFRDLGMFNQALLAKQIWRCLRHPQL 1181

Query: 182  LMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG 217
            L +++LKA Y+P++  L+A  G+  S+V R ++WG
Sbjct: 1182 LCSRVLKASYFPRKGVLEAGCGANASFVSRSLVWG 1216

[Close]

BLAST of Lag0005628 vs. ExPASy TrEMBL
Match: A0A2K3NHG3 (Ribonuclease H (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g025792 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 6.8e-58
Identity = 108/214 (50.47%), Postives = 150/214 (70.09%), Query Frame = 0


Query: 1   MQRILKVYEVATGQVFNFDKSEIAFGTGVTNEVKDFLANILKVKIVQDHGKYLGCSSSLS 60
           +Q I+++Y+ A+GQ+ NF KSE+ F  GV+N+++  ++NIL +KI+    KYLG   +L 
Sbjct: 496 IQNIIQIYQNASGQLVNFSKSEMVFSKGVSNQIQRDISNILPMKILNQFSKYLGMPVALG 555

Query: 61  RNKKASLNYITSKVQKVLQGWKAKLFSTTGKEVLIKVVAQAIPTYTLSCFKLPQGCCHDL 120
           R+KK   N+I  K+ K L+GWK K  S  G+  LIK VAQAIPTY +S F LP+G C  L
Sbjct: 556 RSKKQIFNFIQEKIWKKLKGWKEKHLSFAGRGTLIKAVAQAIPTYLMSSFLLPKGVCDQL 615

Query: 121 NRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDISLFNQALLAKHTWRLLHRPQ 180
            +++ NFWWGSS   RKIHW+KW T+ K K  GG+ FRD+  FN+ALLAK  WRL+ +P 
Sbjct: 616 EKMICNFWWGSSTDHRKIHWIKWSTVCKHKMEGGIGFRDLRAFNEALLAKQGWRLITQPD 675

Query: 181 SLMAQILKAIYYPQEDFLKATLGSRPSYVWRGII 215
           SL+A+ILKA YYP+ +FLKA+   + SY W+ I+
Sbjct: 676 SLVAKILKAKYYPKGEFLKASHKQQMSYTWKSIL 709

[Close]

BLAST of Lag0005628 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 4.5e-30
Identity = 52/113 (46.02%), Postives = 79/113 (69.91%), Query Frame = 0


Query: 101 AIPTYTLSCFKLPQGCCHDLNRLMANFWWGSSEQGRKIHWLKWETMTKLKDRGGMSFRDI 160
           A+PTYT++CF LP+  C  +  ++A+FWW + ++ + +HW  W+ ++  K  GG+ F+DI
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 161 SLFNQALLAKHTWRLLHRPQSLMAQILKAIYYPQEDFLKATLGSRPSYVWRGI 214
             FN ALL K  WR+L RP+SLMA++ K+ Y+ + D L A LGSRPS+VW+ I
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSI 114

[Close]

BLAST of Lag0005628 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 129.0 bits (323), Expect = 4.5e-30
Identity = 53/117 (45.30%), Postives = 80/117 (68.38%), Query Frame = 0


Query: 101 AIPTYTLSCFKLPQGCCHDLNRLMANFWWGSSEQGRKIHWLKWETMTKLK-DRGGMSFRD 160
           A+P Y +SCF+L +  C  L   M  FWW S E  RKI W+ W+ + K K D GG+ FRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 161 ISLFNQALLAKHTWRLLHRPQSLMAQILKAIYYPQEDFLKATLGSRPSYVWRGIIWG 217
           +  FNQALLAK ++R++H+P +L++++L++ Y+P    ++ ++G+RPSY WR II G
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHG 118

The following BLAST results are available for this feature:

BLAST of Lag0005628 vs. NCBI nr


Match Name	E-value	Identity	Description
PNY02790.1	1.4e-57	50.47	ribonuclease H, partial [Trifolium pratense]	[more]
PNX98065.1	1.4e-57	50.47	ribonuclease H [Trifolium pratense]	[more]
PNY02482.1	1.4e-57	50.47	ribonuclease H, partial [Trifolium pratense]	[more]
VVA38592.1	2.4e-57	50.23	PREDICTED: reverse mRNAase, partial [Prunus dulcis]	[more]
PNX92710.1	1.5e-56	49.29	ribonuclease H [Trifolium pratense]	[more]

BLAST of Lag0005628 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93295	6.3e-29	45.30	Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ...	[more]
P0C2F6	2.1e-16	31.65	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of Lag0005628 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A803QQT2	1.1e-60	51.63	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PM68	9.4e-60	49.77	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PWX1	1.2e-59	50.70	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803Q1K6	1.6e-59	50.70	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A2K3NHG3	6.8e-58	50.47	Ribonuclease H (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g025792 PE=4 SV...	[more]

BLAST of Lag0005628 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	4.5e-30	46.02	Ribonuclease H-like superfamily protein	[more]
ATMG00310.1	4.5e-30	45.30	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33116:SF45	SUBFAMILY NOT NAMED	coord: 2..215
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 2..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005628.1	Lag0005628.1	mRNA