Lag0005422 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005422
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	protein SAR DEFICIENT 1
Location	chr6: 17399247 .. 17399845 (-)
RNA-Seq Expression	Lag0005422
Synteny	Lag0005422

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTAGTTCGTTAAGGAATCTTTCAAAAGCATTGGAACCCTTGCTCAGACGAGTGGTACAATTAATGTAGCTTGTATTTTGTGTTTGTATTGTCAAAATTGATTTAGAGCTTTTGTTTAGTTTGTGGTTAATTTTTGGTCCGACAGGTCAATGAGGAAGTCGATCGATGTTTATTGCGCTATTCGAGATCGTTAACGAGAGCTTCGTCGTTGAGGATTCAAGCTCTGGAGCCTTCGAGTTTTCAATTGTATTTGTGAATAAGCTTCCGTCTAAGATGTTTATGGAAATAAGATCACAGATGTGGAAAGTCAGCCGCTACGGATTGCCGTCGAGGACGACGGAGGAGATATGGAGTCACTGATTTCTTCATCTGTTAAAATAGAAATTGTGGTGCTCGATGACGACTTTCCAGCTGGTGATAAAGAGGACTGGACTGCAGAGGAATTCAATGCTAACATTGTGAAGGAGCGCTTCGGAAGGAGACCGTTGCTTCACGGCGACATGAATATCACTCTCCGGCATGGTGCTGCGACCGACACTGGAAACGGGAGGAGGGAGCTCCTCCTCCCTCCTCCGTCTCTTTGTTTTTTTTTTTAA

mRNA sequence

ATGGTTAGTTCGTTAAGGAATCTTTCAAAAGCATTGGAACCCTTGCTCAGACGAGTGGTCAATGAGGAAGTCGATCGATGTTTATTGCGCTATTCGAGATCGTTAACGAGAGCTTCGTCGTTGAGGATTCAAGCTCTGGAGCCTTCGAATGTGGAAAGTCAGCCGCTACGGATTGCCGTCGAGGACGACGGAGGAGATATGGAGTCACTGATTTCTTCATCTGTTAAAATAGAAATTGTGGTGCTCGATGACGACTTTCCAGCTGGTGATAAAGAGGACTGGACTGCAGAGGAATTCAATGCTAACATTGTGAAGGAGCGCTTCGGAAGGAGACCGTTGCTTCACGGCGACATGAATATCACTCTCCGGCATGGTGCTGCGACCGACACTGGAAACGGGAGGAGGGAGCTCCTCCTCCCTCCTCCGTCTCTTTGTTTTTTTTTTTAA

Coding sequence (CDS)

ATGGTTAGTTCGTTAAGGAATCTTTCAAAAGCATTGGAACCCTTGCTCAGACGAGTGGTCAATGAGGAAGTCGATCGATGTTTATTGCGCTATTCGAGATCGTTAACGAGAGCTTCGTCGTTGAGGATTCAAGCTCTGGAGCCTTCGAATGTGGAAAGTCAGCCGCTACGGATTGCCGTCGAGGACGACGGAGGAGATATGGAGTCACTGATTTCTTCATCTGTTAAAATAGAAATTGTGGTGCTCGATGACGACTTTCCAGCTGGTGATAAAGAGGACTGGACTGCAGAGGAATTCAATGCTAACATTGTGAAGGAGCGCTTCGGAAGGAGACCGTTGCTTCACGGCGACATGAATATCACTCTCCGGCATGGTGCTGCGACCGACACTGGAAACGGGAGGAGGGAGCTCCTCCTCCCTCCTCCGTCTCTTTGTTTTTTTTTTTAA

Protein sequence

MVSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEPSNVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEFNANIVKERFGRRPLLHGDMNITLRHGAATDTGNGRRELLLPPPSLCFFF

Homology

[Close]

BLAST of Lag0005422 vs. NCBI nr
Match: XP_022137727.1 (protein SAR DEFICIENT 1 [Momordica charantia])

HSP 1 Score: 189.5 bits (480), Expect = 2.1e-44
Identity = 104/149 (69.80%), Postives = 114/149 (76.51%), Query Frame = 0


Query: 1   MVSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEPS----------- 60
           MV+SLRNLSKALEPLLRRVVNEEV+RCL RY+R LTRASSLRIQALEPS           
Sbjct: 35  MVNSLRNLSKALEPLLRRVVNEEVERCLNRYTRPLTRASSLRIQALEPSSYQLVFLNKLP 94

Query: 61  ----------NVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEF 120
                     +VE QPLR+ +ED GGD  S +  SVKIEIVVLD DFPAGD+E WT EEF
Sbjct: 95  SKIFTGSKITDVEGQPLRLVLEDAGGDPASPMWQSVKIEIVVLDGDFPAGDREYWTPEEF 154

Query: 121 NANIVKERFGRRPLLHGDMNITLRHGAAT 129
           NA+IVKER G+RPLLHGDMNITLRHGAAT
Sbjct: 155 NASIVKERSGKRPLLHGDMNITLRHGAAT 183

[Close]

BLAST of Lag0005422 vs. NCBI nr
Match: KAG7012128.1 (Protein SAR DEFICIENT 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 189.1 bits (479), Expect = 2.7e-44
Identity = 105/149 (70.47%), Postives = 118/149 (79.19%), Query Frame = 0


Query: 1   MVSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEPS----------- 60
           M +SLR+L+KALEPLLRRVV EEVDR L++YSRSLTRASSLRIQALEPS           
Sbjct: 1   MGNSLRHLTKALEPLLRRVVCEEVDRSLMQYSRSLTRASSLRIQALEPSSYQLYFVNNVP 60

Query: 61  ----------NVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEF 120
                     +VE+Q LRIAVED GGD+ SLISSSVK+EIV L+ DFP GDK+DWTA+EF
Sbjct: 61  SKIFTGSKITDVENQALRIAVEDGGGDLPSLISSSVKVEIVALNGDFP-GDKQDWTADEF 120

Query: 121 NANIVKERFGRRPLLHGDMNITLRHGAAT 129
           NANIVKER GRRPLLHGDMN+TLRHGAAT
Sbjct: 121 NANIVKERTGRRPLLHGDMNVTLRHGAAT 148

[Close]

BLAST of Lag0005422 vs. NCBI nr
Match: XP_022994180.1 (protein SAR DEFICIENT 1-like [Cucurbita maxima])

HSP 1 Score: 189.1 bits (479), Expect = 2.7e-44
Identity = 105/149 (70.47%), Postives = 118/149 (79.19%), Query Frame = 0


Query: 1   MVSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEPS----------- 60
           M +SLR+L+KALEPLLRRVV EEVDR L+RYSRSLTRASSLRIQALEPS           
Sbjct: 36  MGNSLRHLTKALEPLLRRVVCEEVDRSLMRYSRSLTRASSLRIQALEPSSYQLYFVNNVP 95

Query: 61  ----------NVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEF 120
                     +VE+Q LRIAVED GGD+ SLISSSVK+EIV L+ DFP GDK++WTA+EF
Sbjct: 96  SKIFTGSKITDVENQALRIAVEDGGGDLPSLISSSVKVEIVALNGDFP-GDKQEWTADEF 155

Query: 121 NANIVKERFGRRPLLHGDMNITLRHGAAT 129
           NANIVKER GRRPLLHGDMN+TLRHGAAT
Sbjct: 156 NANIVKERTGRRPLLHGDMNVTLRHGAAT 183

[Close]

BLAST of Lag0005422 vs. NCBI nr
Match: KAG6572945.1 (Protein SAR DEFICIENT 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 189.1 bits (479), Expect = 2.7e-44
Identity = 105/149 (70.47%), Postives = 118/149 (79.19%), Query Frame = 0


Query: 1   MVSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEPS----------- 60
           M +SLR+L+KALEPLLRRVV EEVDR L++YSRSLTRASSLRIQALEPS           
Sbjct: 36  MGNSLRHLTKALEPLLRRVVCEEVDRSLMQYSRSLTRASSLRIQALEPSSYQLYFVNNVP 95

Query: 61  ----------NVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEF 120
                     +VE+Q LRIAVED GGD+ SLISSSVK+EIV L+ DFP GDK+DWTA+EF
Sbjct: 96  SKIFTGSKITDVENQALRIAVEDGGGDLPSLISSSVKVEIVALNGDFP-GDKQDWTADEF 155

Query: 121 NANIVKERFGRRPLLHGDMNITLRHGAAT 129
           NANIVKER GRRPLLHGDMN+TLRHGAAT
Sbjct: 156 NANIVKERTGRRPLLHGDMNVTLRHGAAT 183

[Close]

BLAST of Lag0005422 vs. NCBI nr
Match: XP_022954519.1 (protein SAR DEFICIENT 1-like [Cucurbita moschata])

HSP 1 Score: 189.1 bits (479), Expect = 2.7e-44
Identity = 105/149 (70.47%), Postives = 118/149 (79.19%), Query Frame = 0


Query: 1   MVSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEPS----------- 60
           M +SLR+L+KALEPLLRRVV EEVDR L++YSRSLTRASSLRIQALEPS           
Sbjct: 36  MGNSLRHLTKALEPLLRRVVCEEVDRSLMQYSRSLTRASSLRIQALEPSSYQLYFVNNVP 95

Query: 61  ----------NVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEF 120
                     +VE+Q LRIAVED GGD+ SLISSSVK+EIV L+ DFP GDK+DWTA+EF
Sbjct: 96  SKIFTGSKITDVENQALRIAVEDGGGDLPSLISSSVKVEIVALNGDFP-GDKQDWTADEF 155

Query: 121 NANIVKERFGRRPLLHGDMNITLRHGAAT 129
           NANIVKER GRRPLLHGDMN+TLRHGAAT
Sbjct: 156 NANIVKERTGRRPLLHGDMNVTLRHGAAT 183

[Close]

BLAST of Lag0005422 vs. ExPASy Swiss-Prot
Match: Q9C9T2 (Protein SAR DEFICIENT 1 OS=Arabidopsis thaliana OX=3702 GN=SARD1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.5e-18
Identity = 57/148 (38.51%), Postives = 88/148 (59.46%), Query Frame = 0


Query: 4   SLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSLRIQALEP--------------- 63
           S   L   LEP++R+VV +EV+  + +  R L+R+SS RI+A E                
Sbjct: 42  SENTLRSVLEPVIRKVVRQEVEYGISKRFR-LSRSSSFRIEAPEATTPTLKLIFRKNLMT 101

Query: 64  --------SNVESQPLRIAVEDDGGDMESLISSSVKIEIVVLDDDFPAGDKEDWTAEEFN 123
                   S+V++ PL I + DD     +L +  +K++IV L  DFP+GDK  WT++EF 
Sbjct: 102 PIFTGSKISDVDNNPLEIILVDDSNKPVNL-NRPIKLDIVALHGDFPSGDK--WTSDEFE 161

Query: 124 ANIVKERFGRRPLLHGDMNITLRHGAAT 129
           +NI+KER G+RPLL G++++T+R+G AT
Sbjct: 162 SNIIKERDGKRPLLAGEVSVTVRNGVAT 185

[Close]

BLAST of Lag0005422 vs. ExPASy Swiss-Prot
Match: C0SV51 (Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.2e-16
Identity = 55/152 (36.18%), Postives = 84/152 (55.26%), Query Frame = 0


Query: 2   VSSLRNLSKALEPLLRRVVNEEVDRCLLRY--SRSLTRASSLRIQALEPSNVESQ----- 61
           + SL+ L  +LEP+LRRVV+EEV+R L +   +R   R+S  RI+ +   N++ Q     
Sbjct: 47  MDSLQRLCSSLEPILRRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRL 106

Query: 62  --PLRIA--VEDDGGDMESLI--------------SSSVKIEIVVLDDDFPAGDKEDWTA 121
             PL     +E + G    ++               +S K+++VVLD DF   D + W+ 
Sbjct: 107 SVPLFTGGKIEGEQGAAIHVVLLDMTTGHVLTVGPEASAKLDVVVLDGDFNTEDDDGWSG 166

Query: 122 EEFNANIVKERFGRRPLLHGDMNITLRHGAAT 129
           EEF  ++VKER G+RPLL GD+ +TL+ G  T
Sbjct: 167 EEFEGHLVKERQGKRPLLTGDVQVTLKEGVGT 198

[Close]

BLAST of Lag0005422 vs. ExPASy Swiss-Prot
Match: Q0WVV6 (Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.1e-15
Identity = 58/154 (37.66%), Postives = 83/154 (53.90%), Query Frame = 0


Query: 2   VSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSL----RIQALEPSNVE----- 61
           V SL+ L  +LEP+LRRVV+EEV+R L +   +    SS+    RI   +  N++     
Sbjct: 30  VDSLQKLCSSLEPILRRVVSEEVERALAKLVPTRLTTSSVFSPKRIGGPDGRNLQLHFKS 89

Query: 62  --SQPLRIA--VEDDGGDMESLI--------------SSSVKIEIVVLDDDFPAGDKEDW 121
             S PL     VE + G    ++               +S+K+E+VVL  DF   D EDW
Sbjct: 90  RLSLPLFTGGRVEGEQGATIHVVLIDANTGRPVTVGPEASLKLEVVVLGGDFNNEDDEDW 149

Query: 122 TAEEFNANIVKERFGRRPLLHGDMNITLRHGAAT 129
           T EEF +++VKER G+RPLL GD+ + L+ G  T
Sbjct: 150 TQEEFESHVVKEREGKRPLLTGDLFVVLKEGVGT 183

[Close]

BLAST of Lag0005422 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.6e-15
Identity = 56/154 (36.36%), Postives = 83/154 (53.90%), Query Frame = 0


Query: 2   VSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASS----LRIQALEPSNVE----- 61
           V SL+ L  +LEP+LRRVV+EE++R L +   +    SS     RI+  +   ++     
Sbjct: 41  VDSLQKLCSSLEPILRRVVSEELERALAKLGPARLTGSSGSSPKRIEGPDGRKLQLHFKS 100

Query: 62  --SQPLRIA--VEDDGGDMESLI--------------SSSVKIEIVVLDDDFPAGDKEDW 121
             S PL     VE + G +  ++               +S K+ IVVL+ DF   D EDW
Sbjct: 101 RLSLPLFTGGKVEGEQGAVIHVVLIDANTGRAVVYGPEASAKLHIVVLEGDFNTEDDEDW 160

Query: 122 TAEEFNANIVKERFGRRPLLHGDMNITLRHGAAT 129
           T EEF +++VKER G+RPLL G++ +TL+ G  T
Sbjct: 161 TQEEFESHVVKERSGKRPLLTGEVYVTLKEGVGT 194

[Close]

BLAST of Lag0005422 vs. ExPASy Swiss-Prot
Match: F4JR57 (Calmodulin-binding protein 60 F OS=Arabidopsis thaliana OX=3702 GN=CBP60F PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 6.7e-14
Identity = 51/153 (33.33%), Postives = 78/153 (50.98%), Query Frame = 0


Query: 2   VSSLRNLSKALEPLLRRVVNEEVDRCLLRYSRSLTRASSL---RIQALEPSNV------- 61
           V SL+ L  +LEPL RR+V+EEV+R + R   S + + S    +IQ L+  N+       
Sbjct: 41  VDSLQRLCSSLEPLFRRIVSEEVERAISRLENSKSTSRSTEPNKIQGLDGRNLQLRFRTR 100

Query: 62  --------------ESQPLRIAVED--DGGDMESLISSSVKIEIVVLDDDFPAGDKEDWT 121
                         +   + + + D   G  +++   S  K+ IVVLD DF   D +DWT
Sbjct: 101 MPPHLFTGGKVEGEQGSAIHVVLIDANTGNVIQTGEESMTKLNIVVLDGDFNDEDDKDWT 160

Query: 122 AEEFNANIVKERFGRRPLLHGDMNITLRHGAAT 129
            E F +  VKER G+RP+L GD ++ ++ G  T
Sbjct: 161 REHFESFEVKEREGKRPILTGDRHVIIKEGVGT 193

The following BLAST results are available for this feature:

BLAST of Lag0005422 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137727.1	2.1e-44	69.80	protein SAR DEFICIENT 1 [Momordica charantia]	[more]
KAG7012128.1	2.7e-44	70.47	Protein SAR DEFICIENT 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022994180.1	2.7e-44	70.47	protein SAR DEFICIENT 1-like [Cucurbita maxima]	[more]
KAG6572945.1	2.7e-44	70.47	Protein SAR DEFICIENT 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022954519.1	2.7e-44	70.47	protein SAR DEFICIENT 1-like [Cucurbita moschata]	[more]

BLAST of Lag0005422 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9T2	4.5e-18	38.51	Protein SAR DEFICIENT 1 OS=Arabidopsis thaliana OX=3702 GN=SARD1 PE=1 SV=1	[more]
C0SV51	3.2e-16	36.18	Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 S...	[more]
Q0WVV6	2.1e-15	37.66	Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 S...	[more]
Q9FKL6	3.6e-15	36.36	Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 S...	[more]
F4JR57	6.7e-14	33.33	Calmodulin-binding protein 60 F OS=Arabidopsis thaliana OX=3702 GN=CBP60F PE=2 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012416	CALMODULIN-BINDING PROTEIN60	PFAM	PF07887	Calmodulin_bind	coord: 52..128 e-value: 4.8E-22 score: 78.6
IPR012416	CALMODULIN-BINDING PROTEIN60	PANTHER	PTHR31713	OS02G0177800 PROTEIN	coord: 3..128
None	No IPR available	PANTHER	PTHR31713:SF42	PROTEIN SAR DEFICIENT 1	coord: 3..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005422.1	Lag0005422.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005516	calmodulin binding