Lag0005250 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005250
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	L-tryptophan--pyruvate aminotransferase 1-like
Location	chr6: 12678860 .. 12679386 (-)
RNA-Seq Expression	Lag0005250
Synteny	Lag0005250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGACAAGTGTAGGGTGGTGATCCCAGGGTGTGATTTGATGAGCTACAGAGCCACCACGGGAAAAGTTTGTTGGTTTCTGTTGCCATTATTGGAGGACGCCATTGCCAGATTGCATCGTCTCGTTGACAACGCCGTGGTTGAAGAAAGACATGTTGTCATGGGGACCAACTCCACGCAGCTCTTCCAAGCTGCCCTTTATGTTCTTTCTACCCCTGCTTCCCTCAAACCTATTAACGTCGTTTTTGCTGCTCCTTACTACTCAGTCTGAAAAAGAACCTTCCCCACTCTTTCTTTTTCTGCTTGGTTTTGCGATCAAACTAAATGATTGGATGTGGTTTTGCGCAGTGTTATCCAGAAGAAACAGACTTGTCCTCGAGGCTTTACAGATGGGCAGAGGATGCCAAGGAATACAATGCCCAAAAAAGTGGGTCATTCGTAGAAGTGATCAACTCCCCCAAACAACCTGGGTTGTTTGCGCCAATCAGTGGTCAAGGACTGCGACCAGGGCAACCTCCTCCATGA

mRNA sequence

ATGGGAGACAAGTGTAGGGTGGTGATCCCAGGGTGTGATTTGATGAGCTACAGAGCCACCACGGGAAAAGTTTGTTGGTTTCTGTTGCCATTATTGGAGGACGCCATTGCCAGATTGCATCGTCTCGTTGACAACGCCGTGGTTGAAGAAAGACATGTTGTCATGGGGACCAACTCCACGCAGCTCTTCCAAGCTGCCCTTTATGTTCTTTCTACCCCTGCTTCCCTCAAACCTATTAACGTCGTTTTTGCTGCTCCTTACTACTCATGTTATCCAGAAGAAACAGACTTGTCCTCGAGGCTTTACAGATGGGCAGAGGATGCCAAGGAATACAATGCCCAAAAAAGTGGGTCATTCGTAGAAGTGATCAACTCCCCCAAACAACCTGGGTTGTTTGCGCCAATCAGTGGTCAAGGACTGCGACCAGGGCAACCTCCTCCATGA

Coding sequence (CDS)

ATGGGAGACAAGTGTAGGGTGGTGATCCCAGGGTGTGATTTGATGAGCTACAGAGCCACCACGGGAAAAGTTTGTTGGTTTCTGTTGCCATTATTGGAGGACGCCATTGCCAGATTGCATCGTCTCGTTGACAACGCCGTGGTTGAAGAAAGACATGTTGTCATGGGGACCAACTCCACGCAGCTCTTCCAAGCTGCCCTTTATGTTCTTTCTACCCCTGCTTCCCTCAAACCTATTAACGTCGTTTTTGCTGCTCCTTACTACTCATGTTATCCAGAAGAAACAGACTTGTCCTCGAGGCTTTACAGATGGGCAGAGGATGCCAAGGAATACAATGCCCAAAAAAGTGGGTCATTCGTAGAAGTGATCAACTCCCCCAAACAACCTGGGTTGTTTGCGCCAATCAGTGGTCAAGGACTGCGACCAGGGCAACCTCCTCCATGA

Protein sequence

MGDKCRVVIPGCDLMSYRATTGKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTNSTQLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETDLSSRLYRWAEDAKEYNAQKSGSFVEVINSPKQPGLFAPISGQGLRPGQPPP

Homology

[Close]

BLAST of Lag0005250 vs. NCBI nr
Match: XP_023538054.1 (L-tryptophan--pyruvate aminotransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 213.0 bits (541), Expect = 1.7e-51
Identity = 104/132 (78.79%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 1   MGDKCRVVIPGCDLMSYRATT--GKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTN 60
           MGDKCRVVIPGC+LMSY+A T  GKVCWFLLP LEDAI RLHRLV NAVV++R++V+GT 
Sbjct: 40  MGDKCRVVIPGCELMSYKAATKPGKVCWFLLPELEDAIVRLHRLVGNAVVDDRYIVVGTG 99

Query: 61  STQLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSG 120
           STQL+QAALY LSTPASLKPINVV AAPYYSCYPEETD L SRLY+WA DA EYNA KSG
Sbjct: 100 STQLYQAALYALSTPASLKPINVVSAAPYYSCYPEETDYLCSRLYKWAGDANEYNANKSG 159

Query: 121 SFVEVINSPKQP 130
            F+EV+NSP  P
Sbjct: 160 PFIEVVNSPNNP 171

[Close]

BLAST of Lag0005250 vs. NCBI nr
Match: XP_022951500.1 (L-tryptophan--pyruvate aminotransferase 1-like [Cucurbita moschata])

HSP 1 Score: 213.0 bits (541), Expect = 1.7e-51
Identity = 104/132 (78.79%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 1   MGDKCRVVIPGCDLMSYRATT--GKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTN 60
           MGDKCRVVIPGC+LMSY+A T  GKVCWFLLP LEDAI RLHRLV NAVV++R++V+GT 
Sbjct: 40  MGDKCRVVIPGCELMSYKAATKPGKVCWFLLPELEDAIVRLHRLVGNAVVDDRYIVVGTG 99

Query: 61  STQLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSG 120
           STQL+QAALY LSTPASLKPINVV AAPYYSCYPEETD L SRLY+WA DA EYNA KSG
Sbjct: 100 STQLYQAALYALSTPASLKPINVVSAAPYYSCYPEETDYLCSRLYKWAGDANEYNANKSG 159

Query: 121 SFVEVINSPKQP 130
            F+EV+NSP  P
Sbjct: 160 PFIEVVNSPNNP 171

[Close]

BLAST of Lag0005250 vs. NCBI nr
Match: KAG6585175.1 (L-tryptophan--pyruvate aminotransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.7e-51
Identity = 104/132 (78.79%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 1   MGDKCRVVIPGCDLMSYRATT--GKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTN 60
           MGDKCRVVIPGC+LMSY+A T  GKVCWFLLP LEDAI RLHRLV NAVV++R++V+GT 
Sbjct: 461 MGDKCRVVIPGCELMSYKAATKPGKVCWFLLPELEDAIVRLHRLVGNAVVDDRYIVVGTG 520

Query: 61  STQLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSG 120
           STQL+QAALY LSTPASLKPINVV AAPYYSCYPEETD L SRLY+WA DA EYNA KSG
Sbjct: 521 STQLYQAALYALSTPASLKPINVVSAAPYYSCYPEETDYLCSRLYKWAGDANEYNANKSG 580

Query: 121 SFVEVINSPKQP 130
            F+EV+NSP  P
Sbjct: 581 PFIEVVNSPNNP 592

[Close]

BLAST of Lag0005250 vs. NCBI nr
Match: KAG7020095.1 (L-tryptophan--pyruvate aminotransferase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 213.0 bits (541), Expect = 1.7e-51
Identity = 104/132 (78.79%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 1   MGDKCRVVIPGCDLMSYRATT--GKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTN 60
           MGDKCRVVIPGC+LMSY+A T  GKVCWFLLP LEDAI RLHRLV NAVV++R++V+GT 
Sbjct: 40  MGDKCRVVIPGCELMSYKAATKPGKVCWFLLPELEDAIVRLHRLVGNAVVDDRYIVVGTG 99

Query: 61  STQLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSG 120
           STQL+QAALY LSTPASLKPINVV AAPYYSCYPEETD L SRLY+WA DA EYNA KSG
Sbjct: 100 STQLYQAALYALSTPASLKPINVVSAAPYYSCYPEETDYLCSRLYKWAGDANEYNANKSG 159

Query: 121 SFVEVINSPKQP 130
            F+EV+NSP  P
Sbjct: 160 PFIEVVNSPNNP 171

[Close]

BLAST of Lag0005250 vs. NCBI nr
Match: XP_023002852.1 (L-tryptophan--pyruvate aminotransferase 1-like [Cucurbita maxima])

HSP 1 Score: 211.1 bits (536), Expect = 6.6e-51
Identity = 103/132 (78.03%), Postives = 114/132 (86.36%), Query Frame = 0


Query: 1   MGDKCRVVIPGCDLMSYRATT--GKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTN 60
           MGDKCRVVIPGC+LMSY+A T  GKVCWFLLP LEDAI RLHRLV NAVV++R++V+GT 
Sbjct: 40  MGDKCRVVIPGCELMSYKAATKPGKVCWFLLPELEDAIVRLHRLVGNAVVDDRYIVVGTG 99

Query: 61  STQLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSG 120
           STQL+QAALY LS PASLKPINVV AAPYYSCYPEETD L SRLY+WA DA EYNA KSG
Sbjct: 100 STQLYQAALYALSNPASLKPINVVSAAPYYSCYPEETDYLCSRLYKWAGDANEYNANKSG 159

Query: 121 SFVEVINSPKQP 130
            F+EV+NSP  P
Sbjct: 160 PFIEVVNSPNNP 171

[Close]

BLAST of Lag0005250 vs. ExPASy Swiss-Prot
Match: Q0DKE8 (Tryptophan aminotransferase-related protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TAR1 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 7.9e-31
Identity = 70/129 (54.26%), Postives = 86/129 (66.67%), Query Frame = 0


Query: 2   GDKCRVVIPGCDLMSYRATTGKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTNSTQ 61
           G++  +VIPG   MSY +  G +CWFL P LE  + RLHRLV NAV +  HV++GT STQ
Sbjct: 95  GERATIVIPGWQTMSYFSDVGSLCWFLEPGLEREVRRLHRLVGNAVADGYHVLVGTGSTQ 154

Query: 62  LFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSGSFV 121
           LFQAALY LS P    P+NVV  APYYS YP  TD L S LYRWA DAK ++     ++V
Sbjct: 155 LFQAALYALSPPGPSAPMNVVSPAPYYSSYPAVTDFLKSGLYRWAGDAKMFDGD---TYV 214

Query: 122 EVINSPKQP 130
           E++ SP  P
Sbjct: 215 ELVCSPSNP 220

[Close]

BLAST of Lag0005250 vs. ExPASy Swiss-Prot
Match: Q9S7N2 (L-tryptophan--pyruvate aminotransferase 1 OS=Arabidopsis thaliana OX=3702 GN=TAA1 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 8.7e-30
Identity = 66/130 (50.77%), Postives = 89/130 (68.46%), Query Frame = 0


Query: 1   MGDKCRVVIPGCDLMSYRATTGKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTNST 60
           MGD+C V I GCDLMSY +    +CWFL P LEDAI  LH +V NA  E+R++V+GT ST
Sbjct: 42  MGDRCTVTIRGCDLMSYFSDMTNLCWFLEPELEDAIKDLHGVVGNAATEDRYIVVGTGST 101

Query: 61  QLFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSGSF 120
           QL QAA++ LS+ A  +P++VV AAP+YS Y EET  + S +Y+W  DA  +   K G +
Sbjct: 102 QLCQAAVHALSSLARSQPVSVVAAAPFYSTYVEETTYVRSGMYKWEGDA--WGFDKKGPY 161

Query: 121 VEVINSPKQP 130
           +E++ SP  P
Sbjct: 162 IELVTSPNNP 169

[Close]

BLAST of Lag0005250 vs. ExPASy Swiss-Prot
Match: Q9LR29 (Tryptophan aminotransferase-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=TAR1 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 8.7e-30
Identity = 65/128 (50.78%), Postives = 87/128 (67.97%), Query Frame = 0


Query: 3   DKCRVVIPGCDLMSYRATTGKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTNSTQL 62
           D+C VVIP  DLMSY + T  VCWFL P LE AI  LH  + NA  EER++V+GT S+QL
Sbjct: 45  DRCTVVIPAWDLMSYFSDTKNVCWFLEPELEKAIKALHGAIGNAATEERYIVVGTGSSQL 104

Query: 63  FQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSGSFVE 122
            QAAL+ LS+ + +KP+++V A PYYS Y EE   L S LY+W  DA+ ++  K G ++E
Sbjct: 105 CQAALFALSSLSEVKPVSIVAAVPYYSTYVEEASYLQSTLYKWEGDARTFD--KKGPYIE 164

Query: 123 VINSPKQP 130
           ++ SP  P
Sbjct: 165 LVTSPNNP 170

[Close]

BLAST of Lag0005250 vs. ExPASy Swiss-Prot
Match: Q94A02 (Tryptophan aminotransferase-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=TAR2 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 65/132 (49.24%), Postives = 84/132 (63.64%), Query Frame = 0


Query: 2   GDKCRVVIPGCDLMSYRATTGKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTNSTQ 61
           G+   +VIPG   +SY +    +CWFL P L   I R+H++V NAV ++R +V+GT STQ
Sbjct: 97  GEVTTMVIPGWQSLSYFSDENNLCWFLEPELAKEIVRVHKVVGNAVTQDRFIVVGTGSTQ 156

Query: 62  LFQAALYVLSTPASLKPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKSGSFV 121
           L+QAALY LS      PINVV A PYYS YP  TD L S LYRW  DAK Y  ++ G ++
Sbjct: 157 LYQAALYALSPHDDSGPINVVSATPYYSTYPLITDCLKSGLYRWGGDAKTY--KEDGPYI 216

Query: 122 EVINSPKQPGLF 133
           E++ SP  P  F
Sbjct: 217 ELVTSPNNPDGF 226

[Close]

BLAST of Lag0005250 vs. ExPASy Swiss-Prot
Match: Q5VQG8 (Tryptophan aminotransferase-related protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TAR2 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.9e-27
Identity = 68/133 (51.13%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 2   GDKCRVVIPGCDLMSYRATTGKVCWFLLPLLEDAIARLHRLVDNAVVEERHVVMGTNSTQ 61
           GD   VVIPG   MSY +    VCWFL P L+  + RLHR+V NA V+  HV++GT STQ
Sbjct: 154 GDAAEVVIPGWQTMSYFSDVTNVCWFLEPELDRQVRRLHRVVGNAAVDGYHVLVGTGSTQ 213

Query: 62  LFQAALYVLSTPASL----KPINVVFAAPYYSCYPEETD-LSSRLYRWAEDAKEYNAQKS 121
           LF AALY L+  A+     +PI+VV  APYYS YP  TD L S L+RWA DA   +A K 
Sbjct: 214 LFMAALYALAPDAAAAAAGEPISVVSTAPYYSSYPAVTDFLRSGLFRWAGDA---DAFKG 273

Query: 122 GSFVEVINSPKQP 130
            S++E++ SP  P
Sbjct: 274 DSYIELVCSPNNP 283

The following BLAST results are available for this feature:

BLAST of Lag0005250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023538054.1	1.7e-51	78.79	L-tryptophan--pyruvate aminotransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022951500.1	1.7e-51	78.79	L-tryptophan--pyruvate aminotransferase 1-like [Cucurbita moschata]	[more]
KAG6585175.1	1.7e-51	78.79	L-tryptophan--pyruvate aminotransferase 1, partial [Cucurbita argyrosperma subsp...	[more]
KAG7020095.1	1.7e-51	78.79	L-tryptophan--pyruvate aminotransferase 1 [Cucurbita argyrosperma subsp. argyros...	[more]
XP_023002852.1	6.6e-51	78.03	L-tryptophan--pyruvate aminotransferase 1-like [Cucurbita maxima]	[more]

BLAST of Lag0005250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0DKE8	7.9e-31	54.26	Tryptophan aminotransferase-related protein 1 OS=Oryza sativa subsp. japonica OX...	[more]
Q9S7N2	8.7e-30	50.77	L-tryptophan--pyruvate aminotransferase 1 OS=Arabidopsis thaliana OX=3702 GN=TAA...	[more]
Q9LR29	8.7e-30	50.78	Tryptophan aminotransferase-related protein 1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q94A02	1.1e-27	49.24	Tryptophan aminotransferase-related protein 2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q5VQG8	6.9e-27	51.13	Tryptophan aminotransferase-related protein 2 OS=Oryza sativa subsp. japonica OX...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006948	Alliinase, C-terminal	PFAM	PF04864	Alliinase_C	coord: 2..129 e-value: 9.6E-35 score: 120.1
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 30..131 e-value: 1.5E-22 score: 82.1
None	No IPR available	PANTHER	PTHR43795	BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED	coord: 1..129
None	No IPR available	PANTHER	PTHR43795:SF15	L-TRYPTOPHAN--PYRUVATE AMINOTRANSFERASE 1	coord: 1..129
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 15..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005250.1	Lag0005250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
molecular_function	GO:0016846	carbon-sulfur lyase activity
molecular_function	GO:0008483	transaminase activity
molecular_function	GO:0003824	catalytic activity