Lag0005238 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005238
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr6: 12557821 .. 12558552 (-)
RNA-Seq Expression	Lag0005238
Synteny	Lag0005238

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATGACGACCCATCAGCCTCTTCTTCAACCCCCCAAAACTACCCGCTCCAAAACCTTCTCCACAATCCTCTCTTTTTCTGCAATTTTCTGCTCAGGCATTTTCATTACCAACAAATTGATCGAATCAAACCCATCTCAAACTGAAAATGATCAGCCCTTCCTTCACACCATCTGCCACAAGGCTTACAACCCATTATCCTGCATTGAAATGGCCGCCTCAGAGTTCCCCTTACACCAAGAAACGAAAACGAAGAACGGGGCTCATTTCTTACAATTTTTCTTGAGCAAATCCATAGCGAAAGTCATTAACACAATCGAGAGCGCCAGAGACCTCAGTCGTAGAATCAACAGCCCTCGCGGGGAAGCGGCTCTGGCCGATTGCGTCGAGCTCATGGAGATCTCGATGGACCGAATAGCGGACTCGATTTCGGCTCTGAAAAACAGAACGAGCGGCTCGATCGAAAACGCGCACACATGGCTGAGCAGCGTGCTGACGAACCACATGACGTGCTCGGATGAGGTCGAGAATTCAATTCCGAGAGCGGCGGGCATGGATCTGGGGCTTGAAGAGCTGATTCTGAGAGGGAGAAATTCACTAGGGATTTTGGTTTGGATTTGGGGATCGGAGATGGAAGAGAATGGGAATGGTATCAATTTAGAGAGAGAGAGAGGAGAGAGAGGAGAGAGAGGAAGTGGGGCGGCGGGATGGTGGTTGTGGGGCTTATGA

mRNA sequence

ATGGCGATGACGACCCATCAGCCTCTTCTTCAACCCCCCAAAACTACCCGCTCCAAAACCTTCTCCACAATCCTCTCTTTTTCTGCAATTTTCTGCTCAGGCATTTTCATTACCAACAAATTGATCGAATCAAACCCATCTCAAACTGAAAATGATCAGCCCTTCCTTCACACCATCTGCCACAAGGCTTACAACCCATTATCCTGCATTGAAATGGCCGCCTCAGAGTTCCCCTTACACCAAGAAACGAAAACGAAGAACGGGGCTCATTTCTTACAATTTTTCTTGAGCAAATCCATAGCGAAAGTCATTAACACAATCGAGAGCGCCAGAGACCTCAGTCGTAGAATCAACAGCCCTCGCGGGGAAGCGGCTCTGGCCGATTGCGTCGAGCTCATGGAGATCTCGATGGACCGAATAGCGGACTCGATTTCGGCTCTGAAAAACAGAACGAGCGGCTCGATCGAAAACGCGCACACATGGCTGAGCAGCGTGCTGACGAACCACATGACGTGCTCGGATGAGGTCGAGAATTCAATTCCGAGAGCGGCGGGCATGGATCTGGGGCTTGAAGAGCTGATTCTGAGAGGGAGAAATTCACTAGGGATTTTGGTTTGGATTTGGGGATCGGAGATGGAAGAGAATGGGAATGGTATCAATTTAGAGAGAGAGAGAGGAGAGAGAGGAGAGAGAGGAAGTGGGGCGGCGGGATGGTGGTTGTGGGGCTTATGA

Coding sequence (CDS)

ATGGCGATGACGACCCATCAGCCTCTTCTTCAACCCCCCAAAACTACCCGCTCCAAAACCTTCTCCACAATCCTCTCTTTTTCTGCAATTTTCTGCTCAGGCATTTTCATTACCAACAAATTGATCGAATCAAACCCATCTCAAACTGAAAATGATCAGCCCTTCCTTCACACCATCTGCCACAAGGCTTACAACCCATTATCCTGCATTGAAATGGCCGCCTCAGAGTTCCCCTTACACCAAGAAACGAAAACGAAGAACGGGGCTCATTTCTTACAATTTTTCTTGAGCAAATCCATAGCGAAAGTCATTAACACAATCGAGAGCGCCAGAGACCTCAGTCGTAGAATCAACAGCCCTCGCGGGGAAGCGGCTCTGGCCGATTGCGTCGAGCTCATGGAGATCTCGATGGACCGAATAGCGGACTCGATTTCGGCTCTGAAAAACAGAACGAGCGGCTCGATCGAAAACGCGCACACATGGCTGAGCAGCGTGCTGACGAACCACATGACGTGCTCGGATGAGGTCGAGAATTCAATTCCGAGAGCGGCGGGCATGGATCTGGGGCTTGAAGAGCTGATTCTGAGAGGGAGAAATTCACTAGGGATTTTGGTTTGGATTTGGGGATCGGAGATGGAAGAGAATGGGAATGGTATCAATTTAGAGAGAGAGAGAGGAGAGAGAGGAGAGAGAGGAAGTGGGGCGGCGGGATGGTGGTTGTGGGGCTTATGA

Protein sequence

MAMTTHQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNGINLERERGERGERGSGAAGWWLWGL

Homology

[Close]

BLAST of Lag0005238 vs. NCBI nr
Match: XP_038881670.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 319.3 bits (817), Expect = 2.8e-83
Identity = 167/232 (71.98%), Postives = 186/232 (80.17%), Query Frame = 0


Query: 3   MTTHQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHK 62
           M THQPLLQPPKT+RSKT  TIL F+A+ CS IFITNK I+SN S  END  FLH +CHK
Sbjct: 1   MATHQPLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKSNSS--ENDLSFLHNLCHK 60

Query: 63  AYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRG 122
           AYNP SCIEMAASEFP  +E KT N  HFLQ FL KSI KVINTI+ A+DLS+RIN+PRG
Sbjct: 61  AYNPSSCIEMAASEFPFPREIKTTNRGHFLQSFLRKSIPKVINTIDRAKDLSQRINNPRG 120

Query: 123 EAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPR 182
           EAALADCVELMEIS  RI DS+SALKNRTSGSIENAHTWLSSVLTNH+TC DEVENS+ R
Sbjct: 121 EAALADCVELMEISNSRIIDSVSALKNRTSGSIENAHTWLSSVLTNHITCWDEVENSVSR 180

Query: 183 AAGMDLGLEELILRGRNSLGILVWIWGSEMEENG------NGINLERERGER 229
           AA MDLGLEELI+RGRNSLG+ V IW SE+E  G      NG     ++G+R
Sbjct: 181 AAAMDLGLEELIMRGRNSLGMFVSIWESEIENMGELEKRVNGYPSWLKKGDR 230

[Close]

BLAST of Lag0005238 vs. NCBI nr
Match: KAG7026620.1 (hypothetical protein SDJN02_10622, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 307.4 bits (786), Expect = 1.1e-79
Identity = 157/215 (73.02%), Postives = 179/215 (83.26%), Query Frame = 0


Query: 3   MTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICH 62
           M TH+PLL QPPKTTR KTF T+L FS + CS IFITNKLI S+PS+     PFLH IC+
Sbjct: 1   MATHEPLLPQPPKTTRPKTFFTVLFFSLLLCSAIFITNKLINSDPSE-NGHHPFLHNICN 60

Query: 63  KAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPR 122
            AYN  SCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIE+A+DL RRIN+PR
Sbjct: 61  MAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIENAKDLRRRINNPR 120

Query: 123 GEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIP 182
           GEAALADCVELMEISMDRI DSI ALK+RT G IENAHTWLSSVLTNH+TCSDE+ENSI 
Sbjct: 121 GEAALADCVELMEISMDRITDSILALKSRTCGPIENAHTWLSSVLTNHVTCSDEIENSIS 180

Query: 183 RAAGMDLGLEELILRGRNSLGILVWIWGSEMEENG 217
           ++A MDLG+EELI+RGRNSLG+LV IWGS  EE G
Sbjct: 181 KSAAMDLGVEELIVRGRNSLGMLVSIWGSGKEEMG 214

[Close]

BLAST of Lag0005238 vs. NCBI nr
Match: KAG6594652.1 (hypothetical protein SDJN03_11205, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 305.1 bits (780), Expect = 5.5e-79
Identity = 156/217 (71.89%), Postives = 179/217 (82.49%), Query Frame = 0


Query: 1   MAMTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTI 60
           + M T +PLL QPPKTTR KTF T+L FS + CS IFITNKLI S+PS+     PFLH I
Sbjct: 135 LPMATREPLLPQPPKTTRPKTFFTVLFFSLLLCSAIFITNKLINSDPSE-NGHHPFLHNI 194

Query: 61  CHKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINS 120
           C+ AYN  SCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIE+A+DL RRIN+
Sbjct: 195 CNMAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIENAKDLRRRINN 254

Query: 121 PRGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENS 180
           PRGEAALADCVELMEISMDRI DSI ALK+RT GSIENAHTWLSSVLTNH+TCSDE+ENS
Sbjct: 255 PRGEAALADCVELMEISMDRITDSILALKSRTCGSIENAHTWLSSVLTNHVTCSDEIENS 314

Query: 181 IPRAAGMDLGLEELILRGRNSLGILVWIWGSEMEENG 217
           I + A MDLG+EE+I+RGRNSLG+LV IWGS  EE G
Sbjct: 315 ISKTAAMDLGVEEVIVRGRNSLGMLVSIWGSGKEEMG 350

[Close]

BLAST of Lag0005238 vs. NCBI nr
Match: XP_023002934.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 299.7 bits (766), Expect = 2.3e-77
Identity = 158/227 (69.60%), Postives = 184/227 (81.06%), Query Frame = 0


Query: 3   MTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICH 62
           M TH+PLL QPP+TTR KTF TIL FS +  S IFITNKLI SNPS+ ++  PFLH IC+
Sbjct: 1   MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDH-HPFLHNICY 60

Query: 63  KAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPR 122
           KAYN  SCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIESA+DL RRIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 123 GEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIP 182
           GEAALADCVELMEISMDRI DSI AL+ R  G+IENAHTWLSSVLTNH+ CSDE+ENSI 
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 183 RAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNGINLERERGER 229
           +AA MDLG+EELI+ GRNSLG+LV IWGS  +E G G     ++G+R
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKE-GMGYPSWLKKGDR 225

[Close]

BLAST of Lag0005238 vs. NCBI nr
Match: TYK13062.1 (pectinesterase [Cucumis melo var. makuwa])

HSP 1 Score: 299.3 bits (765), Expect = 3.0e-77
Identity = 161/238 (67.65%), Postives = 181/238 (76.05%), Query Frame = 0


Query: 3   MTTHQPL--LQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTIC 62
           M THQPL  LQPPKT+ SKT STIL F+ + CS IF+TNK I+ NP   END PFLH IC
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNP--YENDLPFLHNIC 60

Query: 63  HKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSP 122
           HKAYNP SCIEMAASEFPL    KT N  HFLQ FL KS+ KVINTIE A+DL +RINSP
Sbjct: 61  HKAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGE AL DC+ELMEIS  RI DS+ ALKNRTSGSIE++HTWLSSVLTNH+TC DEVE S+
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNGINLERERGERGERGSGAAGW 239
            RAA MDLGLEELI+RGRNSLG+LV IWG E+          +  GE G+RG+G   W
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLEI----------KNLGELGKRGNGYPSW 225

[Close]

BLAST of Lag0005238 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.2e-23
Identity = 79/213 (37.09%), Postives = 116/213 (54.46%), Query Frame = 0


Query: 3   MTTHQP-LLQPPKTTRSKT--FSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTI 62
           M  +QP LL+  K+  SK   F  ++SF+A+  +G         S+P  + +     H I
Sbjct: 1   MEINQPNLLEASKSCYSKITFFLLVISFAALVSTGF--------SSPELSLH-----HKI 60

Query: 63  CHKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINS 122
           C ++ N  SC+ M +    L+      +  + L+ FL K+  ++    E+A D SRRIN+
Sbjct: 61  CDQSVNKESCLAMISEVTGLNM----ADHRNLLKSFLEKTTPRIQKAFETANDASRRINN 120

Query: 123 PRGEAALADCVELMEISMDRIADSISAL--KNRTSGSIENAHTWLSSVLTNHMTCSDEVE 182
           P+   AL DC ELM++S +R+ DSIS L  +N T+ S E+ H WLS VLTNH+TC D +E
Sbjct: 121 PQERTALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLE 180

Query: 183 NSIPR--AAGMDLGLEELILRGRNSLGILVWIW 209
                     M+  L ELILR R SL I V ++
Sbjct: 181 EGSTDYIKTLMESHLNELILRARTSLAIFVTLF 196

[Close]

BLAST of Lag0005238 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 111.3 bits (277), Expect = 1.5e-23
Identity = 73/204 (35.78%), Postives = 108/204 (52.94%), Query Frame = 0


Query: 5   THQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHKAY 64
           ++QPLL  PK+ + K    +LSF AI  S  F T +LI  N +  ++       ICH A+
Sbjct: 4   SNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAH 63

Query: 65  NPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRGEA 124
           +  SC +   SEF     +K  N    L  FL  S+ ++ +T+    +   R N  R +A
Sbjct: 64  DQDSC-QALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDKA 123

Query: 125 ALADCVELMEISMDRIADSISALK--NRTSGSIENAHTWLSSVLTNHMTCSDEVEN-SIP 184
             ADC E+M++S DR+  S+  L+  N    S  N HTWLSSVLTN+MTC + + + S+ 
Sbjct: 124 GFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSVN 183

Query: 185 RAAGMDLGLEELILRGRNSLGILV 206
               +   LE+L+ R R +L I V
Sbjct: 184 SKQIVKPQLEDLVSRARVALAIFV 205

[Close]

BLAST of Lag0005238 vs. ExPASy Swiss-Prot
Match: P09607 (Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 6.7e-19
Identity = 65/201 (32.34%), Postives = 99/201 (49.25%), Query Frame = 0


Query: 4   TTHQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHKA 63
           T  QPLL       S     IL+F       +F+   L+   P Q E        +C  A
Sbjct: 3   TPQQPLLTKTHKQNSIISFKILTFVVTLFVALFLVVFLVA--PYQFEIKH---SNLCKTA 62

Query: 64  YNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRGE 123
            +   C+   +        T   +G   L+ FL  S+ ++ N I   R +  +IN  R +
Sbjct: 63  QDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQINDIREQ 122

Query: 124 AALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRA 183
            AL DC+EL+++S+D + DSI+A+  R+     NA +WLS VLTNH+TC DE++ S  +A
Sbjct: 123 GALTDCLELLDLSVDLVCDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKA 182

Query: 184 AGMDLGLEELILRGRNSLGIL 205
                 L+ELI R + +L +L
Sbjct: 183 MINGTNLDELISRAKVALAML 197

[Close]

BLAST of Lag0005238 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 7.4e-18
Identity = 65/201 (32.34%), Postives = 96/201 (47.76%), Query Frame = 0


Query: 4   TTHQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHKA 63
           T  QPLL       S     IL+F       +F+   L+   P Q E        +C  A
Sbjct: 3   TPQQPLLTKTHKQNSIISFKILTFVVTLFVALFLVVFLVA--PYQFEIKH---SNLCKTA 62

Query: 64  YNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRGE 123
            +   C+   +        T   +G   L  FL   + ++ N I     +  +IN  R E
Sbjct: 63  QDSQLCLSYVSDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQE 122

Query: 124 AALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRA 183
            AL DC+EL++ S+D ++DSI+A+  RT     NA +WLS VLTNH+TC DE++ S  +A
Sbjct: 123 GALTDCLELLDQSVDLVSDSIAAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD-SFTKA 182

Query: 184 AGMDLGLEELILRGRNSLGIL 205
                 L+ELI R + +L +L
Sbjct: 183 MINGTNLDELISRAKVALAML 197

[Close]

BLAST of Lag0005238 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 88.2 bits (217), Expect = 1.4e-16
Identity = 62/195 (31.79%), Postives = 96/195 (49.23%), Query Frame = 0


Query: 11  QPPKTTRSKTFSTILSFSAI-FCSGIFITNKLIESNPSQTENDQPFLHTICHKAYNPLSC 70
           Q P   ++   + I+SF  + F   +F+   L+   P Q E        +C  A +   C
Sbjct: 5   QQPLLIKTHKQNPIISFKILSFVITLFVALFLVA--PYQVEIKH---SNLCKTAQDSQLC 64

Query: 71  IEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRGEAALADC 130
           +   +        T   +G   L  FL   + ++ N I   R +  +IN  R   AL DC
Sbjct: 65  LSYVSDLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDC 124

Query: 131 VELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRAAGMDLG 190
           +EL++ S+D  +DSI+A+  R+     NA +WLS VLTNH+TC DE++ S  +A      
Sbjct: 125 LELLDQSVDFASDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAMINGTN 184

Query: 191 LEELILRGRNSLGIL 205
           LEELI R + +L +L
Sbjct: 185 LEELISRAKVALAML 193

[Close]

BLAST of Lag0005238 vs. ExPASy TrEMBL
Match: A0A6J1KRW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 1.1e-77
Identity = 158/227 (69.60%), Postives = 184/227 (81.06%), Query Frame = 0


Query: 3   MTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICH 62
           M TH+PLL QPP+TTR KTF TIL FS +  S IFITNKLI SNPS+ ++  PFLH IC+
Sbjct: 1   MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDH-HPFLHNICY 60

Query: 63  KAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPR 122
           KAYN  SCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIESA+DL RRIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 123 GEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIP 182
           GEAALADCVELMEISMDRI DSI AL+ R  G+IENAHTWLSSVLTNH+ CSDE+ENSI 
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 183 RAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNGINLERERGER 229
           +AA MDLG+EELI+ GRNSLG+LV IWGS  +E G G     ++G+R
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKE-GMGYPSWLKKGDR 225

[Close]

BLAST of Lag0005238 vs. ExPASy TrEMBL
Match: A0A5D3CNY5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006640 PE=3 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.5e-77
Identity = 161/238 (67.65%), Postives = 181/238 (76.05%), Query Frame = 0


Query: 3   MTTHQPL--LQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTIC 62
           M THQPL  LQPPKT+ SKT STIL F+ + CS IF+TNK I+ NP   END PFLH IC
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNP--YENDLPFLHNIC 60

Query: 63  HKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSP 122
           HKAYNP SCIEMAASEFPL    KT N  HFLQ FL KS+ KVINTIE A+DL +RINSP
Sbjct: 61  HKAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGE AL DC+ELMEIS  RI DS+ ALKNRTSGSIE++HTWLSSVLTNH+TC DEVE S+
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNGINLERERGERGERGSGAAGW 239
            RAA MDLGLEELI+RGRNSLG+LV IWG E+          +  GE G+RG+G   W
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLEI----------KNLGELGKRGNGYPSW 225

[Close]

BLAST of Lag0005238 vs. ExPASy TrEMBL
Match: A0A0A0KHV5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.5e-77
Identity = 160/234 (68.38%), Postives = 184/234 (78.63%), Query Frame = 0


Query: 3   MTTHQP--LLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTIC 62
           M THQP  LLQPPKT+RSKT  T+L F+A+ CS IFITNK I+ NPS  END PFL  IC
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPS--ENDLPFLQNIC 60

Query: 63  HKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSP 122
           HKAY+P SCIEMAASEFPL    KT N   FLQ FL KS+ KVI+TIE A+D+ +RINSP
Sbjct: 61  HKAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGEAALADC+ELMEIS  RI DS+ ALKNRTSGSIEN+HTWLSSVLTNH+TC DEVE+S+
Sbjct: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVWIWG------SEMEENGNGINLERERGER 229
            RAA MDLGLEELI+RGRNSLG+LV IWG       E+E+ GNG     ++G+R
Sbjct: 181 SRAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDR 231

[Close]

BLAST of Lag0005238 vs. ExPASy TrEMBL
Match: A0A1S3B018 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 5.6e-77
Identity = 160/238 (67.23%), Postives = 181/238 (76.05%), Query Frame = 0


Query: 3   MTTHQPL--LQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTIC 62
           M THQPL  LQPPKT+ SKT STIL F+ + CS IF+TNK I+ NP   END PFLH IC
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNP--YENDLPFLHNIC 60

Query: 63  HKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSP 122
           HKAYNP SCIEMAASEFPL    KT N  HFLQ FL KS+ KVINTIE A+DL +RINSP
Sbjct: 61  HKAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGE AL DC+ELMEIS  RI DS+ ALKNRTSG IE++HTWLSSVLTNH+TC DEVE+S+
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNGINLERERGERGERGSGAAGW 239
            RAA MDLGLEELI+RGRNSLG+LV IWG E+          +  GE G+RG+G   W
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLEI----------KNLGELGKRGNGYPSW 225

[Close]

BLAST of Lag0005238 vs. ExPASy TrEMBL
Match: A0A6J1CN37 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 7.1e-64
Identity = 141/220 (64.09%), Postives = 156/220 (70.91%), Query Frame = 0


Query: 3   MTTHQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHK 62
           M T+QPLLQPPKT RSK FS IL F+ I CS  FI N LI  NPS  E DQ FLH IC K
Sbjct: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPS--EIDQLFLHNICRK 60

Query: 63  AYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARD--LSRRINSP 122
           AYNP SC+ MAASE P H+E K  NG HFLQ FL KS+ KV +TIE A D  LSRRIN  
Sbjct: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDA 120

Query: 123 RGEAALADCVELMEISMDRIADSI--SALKNRTSGSIENAHTWLSSVLTNHMTCSDEVEN 182
           RGEAALADC+ELMEISMDRI DSI   AL N+TS  IE+AH WLSSVLTNH+TCSDE+E 
Sbjct: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEK 180

Query: 183 SIPRAAGMDLGLEELILRGRNSLGILVWIWGSEMEENGNG 219
           S+      +LGLEELI+  RNSL +LV I   E E    G
Sbjct: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEG 218

[Close]

BLAST of Lag0005238 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 111.3 bits (277), Expect = 1.1e-24
Identity = 73/204 (35.78%), Postives = 108/204 (52.94%), Query Frame = 0


Query: 5   THQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIESNPSQTENDQPFLHTICHKAY 64
           ++QPLL  PK+ + K    +LSF AI  S  F T +LI  N +  ++       ICH A+
Sbjct: 4   SNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAH 63

Query: 65  NPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPRGEA 124
           +  SC +   SEF     +K  N    L  FL  S+ ++ +T+    +   R N  R +A
Sbjct: 64  DQDSC-QALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDKA 123

Query: 125 ALADCVELMEISMDRIADSISALK--NRTSGSIENAHTWLSSVLTNHMTCSDEVEN-SIP 184
             ADC E+M++S DR+  S+  L+  N    S  N HTWLSSVLTN+MTC + + + S+ 
Sbjct: 124 GFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSVN 183

Query: 185 RAAGMDLGLEELILRGRNSLGILV 206
               +   LE+L+ R R +L I V
Sbjct: 184 SKQIVKPQLEDLVSRARVALAIFV 205

[Close]

BLAST of Lag0005238 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 58.9 bits (141), Expect = 6.4e-09
Identity = 45/147 (30.61%), Postives = 71/147 (48.30%), Query Frame = 0


Query: 43  ESNPSQTENDQP--FLHTICHKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSI 102
           ES PS      P   L  IC     P SCI  + S+ P    T  +            S+
Sbjct: 58  ESTPSPPPELTPSTSLKAICSVTRFPESCIS-SISKLPSSNTTDPET-------LFKLSL 117

Query: 103 AKVINTIESARD----LSRRINSPRGEAALADCVELMEISMDRIADSISAL------KNR 162
             +I+ ++S  D    LS+     R ++AL  C +L+E ++DR+ D++SA+      K  
Sbjct: 118 KVIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTL 177

Query: 163 TSGSIENAHTWLSSVLTNHMTCSDEVE 178
           +S  IE+  TWLS+ +T+H TC D ++
Sbjct: 178 SSSKIEDLKTWLSATVTDHETCFDSLD 196

[Close]

BLAST of Lag0005238 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 56.2 bits (134), Expect = 4.2e-08
Identity = 43/174 (24.71%), Postives = 79/174 (45.40%), Query Frame = 0


Query: 5   THQPLLQPPKTTRSKTFSTILSFSAIFCSGIFITNKLIES---NPSQTENDQPFLHTICH 64
           T  P LQ    T+   F+  +    + C GIF   + ++S    P  T      +   C 
Sbjct: 21  TSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCS 80

Query: 65  KAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSRRINSPR 124
           K+  P  CI+    +FP    + T +    +    + ++ K    + ++  ++     PR
Sbjct: 81  KSLYPNLCID-TLLDFP---GSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPPR 140

Query: 125 GEAALADCVELMEISMDRIADSISALKNRTSG-SIENAHTWLSSVLTNHMTCSD 175
             +A   C+EL++ S+D +  ++S++   +   S  +  TWLSS +TNH TC+D
Sbjct: 141 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTD 190

[Close]

BLAST of Lag0005238 vs. TAIR 10
Match: AT1G11590.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 53.9 bits (128), Expect = 2.1e-07
Identity = 34/81 (41.98%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 131 ELMEISMDRIADSISAL---KNRTSGSIENAHTWLSSVLTNHMTCSDEV-ENSIPRAAGM 190
           E+ME + DR+  S+  L   +    GS EN HTWLSSVLT+++TC DE+ E +  R   +
Sbjct: 98  EMMESTKDRMIRSVEELLGGEFPNRGSYENVHTWLSSVLTSYITCIDEIGEGAYKRR--V 157

Query: 191 DLGLEELILRGRNSLGILVWI 208
           +  LE+LI R R +L + + I
Sbjct: 158 EPKLEDLISRARIALALFISI 176

[Close]

BLAST of Lag0005238 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 52.4 bits (124), Expect = 6.0e-07
Identity = 35/130 (26.92%), Postives = 67/130 (51.54%), Query Frame = 0


Query: 56  LHTICHKAYNPLSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSIAKVINTIESARDLSR 115
           L T+C+    P SCI  + S+ PL +   T +     +  L  +  ++ + +   + L+ 
Sbjct: 459 LRTVCNVTNYPASCIS-SISKLPLSK--TTTDPKVLFRLSLQVTFDELNSIVGLPKKLAE 518

Query: 116 RINSPRGEAALADCVELMEISMDRIADSISAL--------KNRTSGSIENAHTWLSSVLT 175
             N    ++AL+ C ++ ++++D + D+IS+L        KN  S +I +  TWLSS +T
Sbjct: 519 ETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAVT 578

Query: 176 NHMTCSDEVE 178
           +  TC D ++
Sbjct: 579 DIGTCGDTLD 585

The following BLAST results are available for this feature:

BLAST of Lag0005238 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881670.1	2.8e-83	71.98	pectinesterase [Benincasa hispida]	[more]
KAG7026620.1	1.1e-79	73.02	hypothetical protein SDJN02_10622, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6594652.1	5.5e-79	71.89	hypothetical protein SDJN03_11205, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023002934.1	2.3e-77	69.60	pectinesterase-like [Cucurbita maxima]	[more]
TYK13062.1	3.0e-77	67.65	pectinesterase [Cucumis melo var. makuwa]	[more]

BLAST of Lag0005238 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83947	1.2e-23	37.09	Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=20423...	[more]
Q1JPL7	1.5e-23	35.78	Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM...	[more]
P09607	6.7e-19	32.34	Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2	[more]
Q96575	7.4e-18	32.34	Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1	[more]
P14280	1.4e-16	31.79	Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5	[more]

BLAST of Lag0005238 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KRW2	1.1e-77	69.60	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1	[more]
A0A5D3CNY5	1.5e-77	67.65	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00664...	[more]
A0A0A0KHV5	2.5e-77	68.38	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1	[more]
A0A1S3B018	5.6e-77	67.23	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1	[more]
A0A6J1CN37	7.1e-64	64.09	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1	[more]

BLAST of Lag0005238 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11580.1	1.1e-24	35.78	methylesterase PCR A	[more]
AT1G53840.1	6.4e-09	30.61	pectin methylesterase 1	[more]
AT5G53370.1	4.2e-08	24.71	pectin methylesterase PCR fragment F	[more]
AT1G11590.1	2.1e-07	41.98	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G14300.1	6.0e-07	26.92	pectinesterase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 51..203 e-value: 1.1E-15 score: 68.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 55..179 e-value: 3.4E-19 score: 69.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 36..176 e-value: 9.1E-20 score: 68.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 52..203 e-value: 2.4E-25 score: 91.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 54..179
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 17..206
None	No IPR available	PANTHER	PTHR31707:SF333	PECTINESTERASE	coord: 17..206
None	No IPR available	CDD	cd15799	PMEI-like_4	coord: 60..207 e-value: 4.19289E-27 score: 100.177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005238.1	Lag0005238.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity