Lag0005020 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005020
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUnknown protein
Locationchr6: 9622135 .. 9623969 (+)
RNA-Seq ExpressionLag0005020
SyntenyLag0005020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCGGCCGCGACACCAATGTCAGCCCTCTCAAGTTCTCCCGCCTGAGCTGATCTCCGACGAGGATCCCTCCCAGATCTTAGACTTCAGCCGAGCTGTCGCCGGAACTGTTTCCATGTTGAGTTTTCCGACAATTCTTTGAGTGATTCCAGTTTTGATTAGGTTTAAACTCTACCATTGTGTTCTTGCCCAAATTTTTGAAAAAAACCGACCCGATTCGACCCGATTTGAGTCAACACTGAATCGAGGTCGAATTGGTTTGTCCGTACAACTGACCCGACTCGAAAAAATTACACCCCTAATAAAAATAATTTGTTGCAATTAGAGCTTGGAAAACAATTGGTTGAGATAGCACTGTTCAATCAACCAATAAATTCACAACATCTTATAATTTCGTGGCAATGACATTGGAGAAGACTTTTGCTCTTTTTTCCTTTATTCTTGTTCGAGTAATTAAGACTTTGGGTGTTCTTTGCATGTACTTTTTAATTTGTGTGTTAACATTGCTTGTTAAAATAATAATAATAATAATAATATAAATGTATTAATTTATCAGTTCTATGGTTTTTTTCTTACAAAAAAATTAATTACACAAATACTCCTAAACTTTAGGATATGTTTCAATTATGTCCACCAATATAATTTTGAAAAGTTTCATTTTAACTCTTAAACTTTGAAATTGTTCAAAACATGAGACAGTGAATATATAAGAATTGAAAGATATGTGACATTATTTACAACATCTTTCCTTCACAAAAATAGAGGTGGTGTTAATGGAAAAAATTATTGTATATTAAATGATGGTGTAAAATGATAATAACATATGTTTTGAGTTTAAATGATTTATGACAACAATCATCTTACTTCTTATATAGTTGAAATTGATGTTTAAATGATATCAAACCTTCCCATTTGTGTAAAGATCAACTACCACACCTTTGCAGGTTTAGGGTGGAATCAACTAGCCATGTATTTTGGCAGTGTAGGTTTGCAAAAAAAAAAAAAATTGGCAATTTTTCTTCCCTGACTTATTCGGTTTTTCAATTCGTGTAGGGATAATTGGACTCCGATGGACTAATGGGAAGGAATGACTCAAAACCTCAGCACGCAAGACATGTCTAAAGCAGCAATTATTTTATGGTACATATGGAAAGATAGAAACGAGTGCAATCACAGTGGACAGAGACCAAATTTGGAGAAATCAGTGAATGTGATCTCCATTCATTTAGAAGAATTGGAAGGATCAAATGAAACTAACCTGAGTTCAGTTCAGAGGGAGAGCCTTTCGAGTCATGCGAGTTGGAGGCCTCCGGAGGAAAACTTCTGGAAGCTCAATGTCGATGCCTCTTGGTGTGCGAAGTCAAACACCGAAAGTGTGGGTGGATTCTTCGTGACTCAAACGAATCTCTGATCAAAGCTGGATGCAAAAGAATTACTCAAAATTGGGAGATTAAAATCCTTGAGGCAAATGTCATTCTCACGGGCATCAAAGCTTACCTGGAAAACTCTGAAGGTAGGGAATCCGTGTTTAAGCCCCCGTTGGTGGTTGAATCAGATTCTGCAGTGGTGATCACGACGCTCAACGGTGAGAGGGAAGATCGTTCGGAATTAGCTATGTTCACCAAATCCATTCTTGATCTTGCTCCCTCTGCTAATGTCAATAGTTTCTCTAAATGTAATAGAGTCGAAAACAATGGGGCTCACAATCTTGCTCGATGGGGAGCAAAGATGGGGATTTTTTTGGTCTGGGCGCATCTTGTTCCGAGAAGTAAAACGTGCATTTTTTTTTGGAGGGAGGAGGATCTCTCCCATGGTTATTTCGTGTATTGTTGA

mRNA sequence

ATGCCTCGGCCGCGACACCAATGTCAGCCCTCTCAAGTTCTCCCGCCTGAGCTGATCTCCGACGAGGATCCCTCCCAGATCTTAGACTTCAGCCGAGCTGTCGCCGGAACTGTTTCCATTTCAGAGGGAGAGCCTTTCGAGTCATGCGAGTTGGAGGCCTCCGGAGGAAAACTTCTGGAAGCTCAATGTCGATGCCTCTTGGTGTGCGAAGTCAAACACCGAAAGTGTGGGTGGATTCTTCGTGACTCAAACGAATCTCTGATCAAAGCTGGATGCAAAAGAATTACTCAAAATTGGGAGATTAAAATCCTTGAGGCAAATGTCATTCTCACGGGCATCAAAGCTTACCTGGAAAACTCTGAAGGTAGGGAATCCGTGTTTAAGCCCCCGTTGGTGGTTGAATCAGATTCTGCAGTGGTGATCACGACGCTCAACGGTGAGAGGGAAGATCGTTCGGAATTAGCTATGTTCACCAAATCCATTCTTGATCTTGCTCCCTCTGCTAATGTCAATAGTTTCTCTAAATGTAATAGAGTCGAAAACAATGGGGCTCACAATCTTGCTCGATGGGGAGCAAAGATGGGGATTTTTTTGGTCTGGGCGCATCTTGTTCCGAGAAGTAAAACGTGCATTTTTTTTTGGAGGGAGGAGGATCTCTCCCATGGTTATTTCGTGTATTGTTGA

Coding sequence (CDS)

ATGCCTCGGCCGCGACACCAATGTCAGCCCTCTCAAGTTCTCCCGCCTGAGCTGATCTCCGACGAGGATCCCTCCCAGATCTTAGACTTCAGCCGAGCTGTCGCCGGAACTGTTTCCATTTCAGAGGGAGAGCCTTTCGAGTCATGCGAGTTGGAGGCCTCCGGAGGAAAACTTCTGGAAGCTCAATGTCGATGCCTCTTGGTGTGCGAAGTCAAACACCGAAAGTGTGGGTGGATTCTTCGTGACTCAAACGAATCTCTGATCAAAGCTGGATGCAAAAGAATTACTCAAAATTGGGAGATTAAAATCCTTGAGGCAAATGTCATTCTCACGGGCATCAAAGCTTACCTGGAAAACTCTGAAGGTAGGGAATCCGTGTTTAAGCCCCCGTTGGTGGTTGAATCAGATTCTGCAGTGGTGATCACGACGCTCAACGGTGAGAGGGAAGATCGTTCGGAATTAGCTATGTTCACCAAATCCATTCTTGATCTTGCTCCCTCTGCTAATGTCAATAGTTTCTCTAAATGTAATAGAGTCGAAAACAATGGGGCTCACAATCTTGCTCGATGGGGAGCAAAGATGGGGATTTTTTTGGTCTGGGCGCATCTTGTTCCGAGAAGTAAAACGTGCATTTTTTTTTGGAGGGAGGAGGATCTCTCCCATGGTTATTTCGTGTATTGTTGA

Protein sequence

MPRPRHQCQPSQVLPPELISDEDPSQILDFSRAVAGTVSISEGEPFESCELEASGGKLLEAQCRCLLVCEVKHRKCGWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAYLENSEGRESVFKPPLVVESDSAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLARWGAKMGIFLVWAHLVPRSKTCIFFWREEDLSHGYFVYC
Homology
BLAST of Lag0005020 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-08
Identity = 40/129 (31.01%), Postives = 68/129 (52.71%), Query Frame = 0

Query: 77  GWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAYLENSEGRESVFKPPLVVESD 136
           GWI+R  +  ++ AG + +     +K+LEA+ IL G++        R      PL +E+D
Sbjct: 97  GWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLR------PLHIETD 156

Query: 137 SAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLARWGAKMGI 196
           SA V + LN +RED ++     + IL+L  S  + +F+K  R  N  AH+LA+  + +  
Sbjct: 157 SAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRASVLRE 216

Query: 197 FLVWAHLVP 206
            ++W    P
Sbjct: 217 SMIWVDCFP 219

BLAST of Lag0005020 vs. NCBI nr
Match: KAG6590166.1 (hypothetical protein SDJN03_15589, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-07
Identity = 36/50 (72.00%), Postives = 40/50 (80.00%), Query Frame = 0

Query: 1  MPRPRHQCQPSQVLPPELISDEDPSQILDFSRAVAGTVSISEGEPFESCE 51
          MPRPRHQ QPSQVLPPELIS  DPSQILDF +AVA TVS   G+P ++ E
Sbjct: 1  MPRPRHQRQPSQVLPPELISGVDPSQILDFGQAVARTVS---GQPHKATE 47

BLAST of Lag0005020 vs. NCBI nr
Match: KAG6588222.1 (hypothetical protein SDJN03_16787, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 65.9 bits (159), Expect = 5.3e-07
Identity = 34/48 (70.83%), Postives = 37/48 (77.08%), Query Frame = 0

Query: 1  MPRPRHQCQPSQVLPPELISDEDPSQILDFSRAVAGTVSISEGEPFES 49
          MPRPRH  QPSQVLPPELIS EDPSQILDF R VAG  S   G+P ++
Sbjct: 1  MPRPRHHRQPSQVLPPELISGEDPSQILDFERPVAGAFS---GQPHQA 45

BLAST of Lag0005020 vs. NCBI nr
Match: KGN66547.1 (hypothetical protein Csa_007065 [Cucumis sativus])

HSP 1 Score: 62.4 bits (150), Expect = 5.8e-06
Identity = 34/54 (62.96%), Postives = 39/54 (72.22%), Query Frame = 0

Query: 1  MPRPRHQCQPSQVLPPELISDEDPSQILDFSRAVAGTVSISEGEPFESCELEAS 55
          MPRPRHQ Q SQVLPP+ IS EDPSQILDFS  VAG VS   G+P +  +  A+
Sbjct: 1  MPRPRHQRQLSQVLPPDFISAEDPSQILDFSIGVAGAVS---GQPHQPSQATAN 51

BLAST of Lag0005020 vs. NCBI nr
Match: KMS97072.1 (hypothetical protein BVRB_7g179330 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.4 bits (150), Expect = 5.8e-06
Identity = 35/124 (28.23%), Postives = 61/124 (49.19%), Query Frame = 0

Query: 77  GWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAYLENSEGRESVFKPPLVVESD 136
           G + RD N  +++A  + ++ +W  +  EA  +L   +  +  ++G  +V     V+ESD
Sbjct: 678 GMVARDENGQVLRAWSRTMSHDWPAEKAEAAAVLWATEEAI--AQGWSNV-----VLESD 737

Query: 137 SAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLARWGAKMGI 196
           + V+I  +N   + R ++    + +L+L PS     FS C R  N  AH LA+W      
Sbjct: 738 AQVIINAINSRLQRRGDIQAMLEDVLNLVPSFETIKFSFCYRECNEIAHRLAKWATSNFC 794

Query: 197 FLVW 201
             VW
Sbjct: 798 DEVW 794

BLAST of Lag0005020 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-08
Identity = 40/129 (31.01%), Postives = 68/129 (52.71%), Query Frame = 0

Query: 77  GWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAYLENSEGRESVFKPPLVVESD 136
           GWI+R  +  ++ AG + +     +K+LEA+ IL G++        R      PL +E+D
Sbjct: 97  GWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLR------PLHIETD 156

Query: 137 SAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLARWGAKMGI 196
           SA V + LN +RED ++     + IL+L  S  + +F+K  R  N  AH+LA+  + +  
Sbjct: 157 SAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRASVLRE 216

Query: 197 FLVWAHLVP 206
            ++W    P
Sbjct: 217 SMIWVDCFP 219

BLAST of Lag0005020 vs. ExPASy TrEMBL
Match: A0A0A0M0A1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629040 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.8e-06
Identity = 34/54 (62.96%), Postives = 39/54 (72.22%), Query Frame = 0

Query: 1  MPRPRHQCQPSQVLPPELISDEDPSQILDFSRAVAGTVSISEGEPFESCELEAS 55
          MPRPRHQ Q SQVLPP+ IS EDPSQILDFS  VAG VS   G+P +  +  A+
Sbjct: 1  MPRPRHQRQLSQVLPPDFISAEDPSQILDFSIGVAGAVS---GQPHQPSQATAN 51

BLAST of Lag0005020 vs. ExPASy TrEMBL
Match: A0A0J8BAU9 (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris OX=3555 GN=BVRB_7g179330 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.8e-06
Identity = 35/124 (28.23%), Postives = 61/124 (49.19%), Query Frame = 0

Query: 77  GWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAYLENSEGRESVFKPPLVVESD 136
           G + RD N  +++A  + ++ +W  +  EA  +L   +  +  ++G  +V     V+ESD
Sbjct: 678 GMVARDENGQVLRAWSRTMSHDWPAEKAEAAAVLWATEEAI--AQGWSNV-----VLESD 737

Query: 137 SAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLARWGAKMGI 196
           + V+I  +N   + R ++    + +L+L PS     FS C R  N  AH LA+W      
Sbjct: 738 AQVIINAINSRLQRRGDIQAMLEDVLNLVPSFETIKFSFCYRECNEIAHRLAKWATSNFC 794

Query: 197 FLVW 201
             VW
Sbjct: 798 DEVW 794

BLAST of Lag0005020 vs. ExPASy TrEMBL
Match: A0A803MUM1 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.1e-05
Identity = 35/129 (27.13%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 77  GWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAYLENSEGRESVFKPPLVVESD 136
           G ++RD    +++AGC+++ Q+W+  I+EA  ++ G+K  ++ +  R       ++VESD
Sbjct: 187 GVVVRDHLGEVVRAGCQQVHQSWDASIVEAKAMVLGLKLAMQCNVKR-------VIVESD 246

Query: 137 SAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLARWGAKMGI 196
              ++  +NG  +D S L +  K I  +A   +   F+   R  N+ AH +A        
Sbjct: 247 CERLVKLVNGVGKDGSFLGLLAKEIKCVAEFFDSIVFAHVFREANSVAHAMAHLSPLEYS 306

Query: 197 FLVWAHLVP 206
             VW    P
Sbjct: 307 TRVWVWCCP 308

BLAST of Lag0005020 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.1e-05
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 0

Query: 77  GWILRDSNESLIKAGCKRITQNWEIKILEANVILTGIKAY-LENSEGRESVFKPPLVVES 136
           GWILRD    +IKA C+ I     I  LE   I  G++A   E+    +     P+ +ES
Sbjct: 105 GWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLES 164

Query: 137 DSAVVITTLNGEREDRSELAMFTKSILDLAPSANVNSFSKCNRVENNGAHNLAR 190
           DS   I  L+ + +D++E+    + I  +     + S    +R  N  AH+LAR
Sbjct: 165 DSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAHDLAR 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155262.12.8e-0831.01uncharacterized protein LOC111022403 [Momordica charantia][more]
KAG6590166.11.8e-0772.00hypothetical protein SDJN03_15589, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6588222.15.3e-0770.83hypothetical protein SDJN03_16787, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN66547.15.8e-0662.96hypothetical protein Csa_007065 [Cucumis sativus][more]
KMS97072.15.8e-0628.23hypothetical protein BVRB_7g179330 [Beta vulgaris subsp. vulgaris][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DNV91.4e-0831.01uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A0A0M0A12.8e-0662.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629040 PE=4 SV=1[more]
A0A0J8BAU92.8e-0628.23Uncharacterized protein OS=Beta vulgaris subsp. vulgaris OX=3555 GN=BVRB_7g17933... [more]
A0A803MUM13.1e-0527.13Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A6J1DSV14.1e-0531.58uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 75..190
e-value: 1.1E-12
score: 47.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 68..193
e-value: 3.2E-6
score: 29.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005020.1Lag0005020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity