Lag0004629 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004629
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr6: 5589230 .. 5590549 (+)
RNA-Seq ExpressionLag0004629
SyntenyLag0004629
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTCCAAAACAGCGAACCAGAAAAAGAAAATGGGAGAATCCAAATTGTCACAACTGATCCAGTGGTAGCATCCATCAATCGAATTCTTCGACAAGTTCCATCTTATTCTTCGGATCACGTCAGCATTTACAGGGTTCCAGAGCCTCTGCGCAGCACCAAGCCAGAGGCTTATACTCCCACACTCATCTCTATCGGCCCTTTTCACTCTGGTAGAGAAGACCTCATGGCCAATGCACACAAACCCAGATACCTTCAAAGCTTCCTCGACCTCACCAAACTCACCACGAACACGATCATAGAACGAGTCAAGACTTTGGAAGATAGAGCTCGCCGTTCCTACGCAGAATCCATCAAGATGAACAGAGATGAATTCCTGCAACTCCTCGTCTTGGATGGCTGTTTCGTCATCATGCATTTGATTGGTTCTCAGTTCTTACACCTTCGATCCTCCAACTGTTCTCGTTTATGGAAATTCTGGGATGAAATTTTTTGCGATCTCTTACTGCTCGAAAACCAGCTTCCCTTCTTCCTTCTCCAATCTCTGTACGACCTCTGCGCCTCCTCTCAACCTTCACTGGAAAGATTGAGTTTCATTGAACTTGTCCGTCAGTATTTCATCGAGGACTCCAATTCAGAGGGGTTGGATTTTCTCGAGAGAAACGTTTCGGCAGAAATATGCCAAGTGAATCACTTTGTTGATTTTGTAAGAATGCACTATACTCATACATGCTCTTCAAAATTGGAGTTCTTCTGGCCCACGTTCTGGCCGCCGACTGCCACTGAGCTTCACCAATGCGGCGTTTATTTCAAGACAGAGAAGGAGAAAAAGAAATGTGCTGTGGACTTAGCGTTTAGAGACCAAAGTGGCTGTCTAGAATTGCCAAAAATCATCATATACGACGGTTTCGAAACGCGTGTGAGGAACCTCATAGCTTACGAGCAATGCCATGTCGGCGACATGAGCAACGAGGTGAGCAACTTCGCTGTCTTTATGCAGTACTTGGTCCAGACAGACCAAGACGTGAAATTGCTGATTGAGGATGGGATCATACAGAACAATGTGGGCAGTGTCAATGAGGTTACTCAGTTATTCAATAGCCTCTGTAAGCATGTTGCTCCTGGGAACAACTTTTACAACTATGAGTGCCAGAGAATGAAGGAATTCTGCAAGCGTCGTCGACATCGGTGGCTGGCTTCGTTGAGACGCAACTATTTCAGCACGCCGTGGACTTGTGCTTCCTCCATTGCTGCCATCCTCCTCCTTGTTCTCACTTTCGTGCAAACGGTCGTCGCTGTAGTCGCCTTGTATAAGTGA

mRNA sequence

ATGGAACTCCAAAACAGCGAACCAGAAAAAGAAAATGGGAGAATCCAAATTGTCACAACTGATCCAGTGGTAGCATCCATCAATCGAATTCTTCGACAAGTTCCATCTTATTCTTCGGATCACGTCAGCATTTACAGGGTTCCAGAGCCTCTGCGCAGCACCAAGCCAGAGGCTTATACTCCCACACTCATCTCTATCGGCCCTTTTCACTCTGGTAGAGAAGACCTCATGGCCAATGCACACAAACCCAGATACCTTCAAAGCTTCCTCGACCTCACCAAACTCACCACGAACACGATCATAGAACGAGTCAAGACTTTGGAAGATAGAGCTCGCCGTTCCTACGCAGAATCCATCAAGATGAACAGAGATGAATTCCTGCAACTCCTCGTCTTGGATGGCTGTTTCGTCATCATGCATTTGATTGGTTCTCAGTTCTTACACCTTCGATCCTCCAACTGTTCTCGTTTATGGAAATTCTGGGATGAAATTTTTTGCGATCTCTTACTGCTCGAAAACCAGCTTCCCTTCTTCCTTCTCCAATCTCTGTACGACCTCTGCGCCTCCTCTCAACCTTCACTGGAAAGATTGAGTTTCATTGAACTTGTCCGTCAGTATTTCATCGAGGACTCCAATTCAGAGGGGTTGGATTTTCTCGAGAGAAACGTTTCGGCAGAAATATGCCAAGTGAATCACTTTGTTGATTTTGTAAGAATGCACTATACTCATACATGCTCTTCAAAATTGGAGTTCTTCTGGCCCACGTTCTGGCCGCCGACTGCCACTGAGCTTCACCAATGCGGCGTTTATTTCAAGACAGAGAAGGAGAAAAAGAAATGTGCTGTGGACTTAGCGTTTAGAGACCAAAGTGGCTGTCTAGAATTGCCAAAAATCATCATATACGACGGTTTCGAAACGCGTGTGAGGAACCTCATAGCTTACGAGCAATGCCATGTCGGCGACATGAGCAACGAGGTGAGCAACTTCGCTGTCTTTATGCAGTACTTGGTCCAGACAGACCAAGACGTGAAATTGCTGATTGAGGATGGGATCATACAGAACAATGTGGGCAGTGTCAATGAGGTTACTCAGTTATTCAATAGCCTCTGTAAGCATGTTGCTCCTGGGAACAACTTTTACAACTATGAGTGCCAGAGAATGAAGGAATTCTGCAAGCGTCGTCGACATCGGTGGCTGGCTTCGTTGAGACGCAACTATTTCAGCACGCCGTGGACTTGTGCTTCCTCCATTGCTGCCATCCTCCTCCTTGTTCTCACTTTCGTGCAAACGGTCGTCGCTGTAGTCGCCTTGTATAAGTGA

Coding sequence (CDS)

ATGGAACTCCAAAACAGCGAACCAGAAAAAGAAAATGGGAGAATCCAAATTGTCACAACTGATCCAGTGGTAGCATCCATCAATCGAATTCTTCGACAAGTTCCATCTTATTCTTCGGATCACGTCAGCATTTACAGGGTTCCAGAGCCTCTGCGCAGCACCAAGCCAGAGGCTTATACTCCCACACTCATCTCTATCGGCCCTTTTCACTCTGGTAGAGAAGACCTCATGGCCAATGCACACAAACCCAGATACCTTCAAAGCTTCCTCGACCTCACCAAACTCACCACGAACACGATCATAGAACGAGTCAAGACTTTGGAAGATAGAGCTCGCCGTTCCTACGCAGAATCCATCAAGATGAACAGAGATGAATTCCTGCAACTCCTCGTCTTGGATGGCTGTTTCGTCATCATGCATTTGATTGGTTCTCAGTTCTTACACCTTCGATCCTCCAACTGTTCTCGTTTATGGAAATTCTGGGATGAAATTTTTTGCGATCTCTTACTGCTCGAAAACCAGCTTCCCTTCTTCCTTCTCCAATCTCTGTACGACCTCTGCGCCTCCTCTCAACCTTCACTGGAAAGATTGAGTTTCATTGAACTTGTCCGTCAGTATTTCATCGAGGACTCCAATTCAGAGGGGTTGGATTTTCTCGAGAGAAACGTTTCGGCAGAAATATGCCAAGTGAATCACTTTGTTGATTTTGTAAGAATGCACTATACTCATACATGCTCTTCAAAATTGGAGTTCTTCTGGCCCACGTTCTGGCCGCCGACTGCCACTGAGCTTCACCAATGCGGCGTTTATTTCAAGACAGAGAAGGAGAAAAAGAAATGTGCTGTGGACTTAGCGTTTAGAGACCAAAGTGGCTGTCTAGAATTGCCAAAAATCATCATATACGACGGTTTCGAAACGCGTGTGAGGAACCTCATAGCTTACGAGCAATGCCATGTCGGCGACATGAGCAACGAGGTGAGCAACTTCGCTGTCTTTATGCAGTACTTGGTCCAGACAGACCAAGACGTGAAATTGCTGATTGAGGATGGGATCATACAGAACAATGTGGGCAGTGTCAATGAGGTTACTCAGTTATTCAATAGCCTCTGTAAGCATGTTGCTCCTGGGAACAACTTTTACAACTATGAGTGCCAGAGAATGAAGGAATTCTGCAAGCGTCGTCGACATCGGTGGCTGGCTTCGTTGAGACGCAACTATTTCAGCACGCCGTGGACTTGTGCTTCCTCCATTGCTGCCATCCTCCTCCTTGTTCTCACTTTCGTGCAAACGGTCGTCGCTGTAGTCGCCTTGTATAAGTGA

Protein sequence

MELQNSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLYDLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNVSAEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAYEQCHVGDMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQTVVAVVALYK
Homology
BLAST of Lag0004629 vs. NCBI nr
Match: XP_022961897.1 (UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961899.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.7e-155
Identity = 294/436 (67.43%), Postives = 344/436 (78.90%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS  E EN   +  T DPVV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 40  NSLAEIEN---ETGTFDPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 99

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DL AN+ KP YLQ+FL+LTKL TNTI+E VKT E RAR  YAESI+MNRD
Sbjct: 100 SIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCYAESIEMNRD 159

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLIG  F  LR+S+ S LWKFW E+FCDL+LLENQLPFFLLQSLY
Sbjct: 160 EFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLY 219

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNV--SAEICQVNHFVDFVRMHYT 244
           DLCASSQP L+ + FIELV QYFIE S+  GL  L+ +V  +    QVNHFVD +R+H+T
Sbjct: 220 DLCASSQPLLKGVHFIELVHQYFIE-SHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFT 279

Query: 245 HTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYD 304
           HT S +   F  TFWPP AT+LH+CGV FK  K        +AF+DQ GCL+LP+I IYD
Sbjct: 280 HTRSDETS-FQHTFWPPNATKLHECGVIFKMGK-------GIAFKDQGGCLQLPQINIYD 339

Query: 305 GFETRVRNLIAYEQCHVG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNE 364
            FE RVRNLIAYEQCH+G ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NE
Sbjct: 340 DFEKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINE 399

Query: 365 VTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 424
           VTQLFN+L KH+ PG N YN++C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAIL
Sbjct: 400 VTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAIL 459

Query: 425 LLVLTFVQTVVAVVAL 438
           LL LT +QT+VA+V L
Sbjct: 460 LLALTLIQTIVAIVDL 463

BLAST of Lag0004629 vs. NCBI nr
Match: XP_022961893.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961894.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961895.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.7e-155
Identity = 294/436 (67.43%), Postives = 344/436 (78.90%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS  E EN   +  T DPVV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 139 NSLAEIEN---ETGTFDPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 198

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DL AN+ KP YLQ+FL+LTKL TNTI+E VKT E RAR  YAESI+MNRD
Sbjct: 199 SIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCYAESIEMNRD 258

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLIG  F  LR+S+ S LWKFW E+FCDL+LLENQLPFFLLQSLY
Sbjct: 259 EFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLY 318

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNV--SAEICQVNHFVDFVRMHYT 244
           DLCASSQP L+ + FIELV QYFIE S+  GL  L+ +V  +    QVNHFVD +R+H+T
Sbjct: 319 DLCASSQPLLKGVHFIELVHQYFIE-SHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFT 378

Query: 245 HTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYD 304
           HT S +   F  TFWPP AT+LH+CGV FK  K        +AF+DQ GCL+LP+I IYD
Sbjct: 379 HTRSDETS-FQHTFWPPNATKLHECGVIFKMGK-------GIAFKDQGGCLQLPQINIYD 438

Query: 305 GFETRVRNLIAYEQCHVG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNE 364
            FE RVRNLIAYEQCH+G ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NE
Sbjct: 439 DFEKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINE 498

Query: 365 VTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 424
           VTQLFN+L KH+ PG N YN++C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAIL
Sbjct: 499 VTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAIL 558

Query: 425 LLVLTFVQTVVAVVAL 438
           LL LT +QT+VA+V L
Sbjct: 559 LLALTLIQTIVAIVDL 562

BLAST of Lag0004629 vs. NCBI nr
Match: XP_023546104.1 (UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023546105.1 UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.4 bits (1435), Expect = 1.1e-154
Identity = 293/436 (67.20%), Postives = 345/436 (79.13%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS+ E EN   +  T+D VV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 40  NSQAEIEN---ETGTSDLVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 99

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DLMAN+ KPRYLQ+FL+LT+L TNTI+E VKT E RAR  YAESI+M+RD
Sbjct: 100 SIGPLHSGRKDLMANSLKPRYLQNFLNLTQLPTNTIVEIVKTWEKRARYCYAESIEMSRD 159

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLI   F  LR+S+ S LWKFWDE+FCDL+LLENQLPFFLLQSLY
Sbjct: 160 EFVELLVFDGCFVVMHLISYSFFELRASDMSNLWKFWDELFCDLILLENQLPFFLLQSLY 219

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNV--SAEICQVNHFVDFVRMHYT 244
           DLCASSQP  + + FIELV QYFIE S+  GL  L+ +V  +    QVNHFVD +R+H+T
Sbjct: 220 DLCASSQPFSKGVRFIELVHQYFIE-SHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFT 279

Query: 245 HTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYD 304
           HT S K+  F  TFWPP AT+LH+CGV FKT K        +AF+DQ GCL+LP+I IYD
Sbjct: 280 HTRSDKMS-FQHTFWPPNATQLHECGVIFKTGK-------GIAFKDQGGCLQLPQINIYD 339

Query: 305 GFETRVRNLIAYEQCH-VGDMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNE 364
            FE RVRNLIAYEQCH   ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NE
Sbjct: 340 DFEKRVRNLIAYEQCHFCSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINE 399

Query: 365 VTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 424
           VTQLFN+L KH+ PG N YN +C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAIL
Sbjct: 400 VTQLFNNLGKHICPGINSYNSDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAIL 459

Query: 425 LLVLTFVQTVVAVVAL 438
           LL LT +QT+VA+V L
Sbjct: 460 LLALTLIQTIVALVDL 463

BLAST of Lag0004629 vs. NCBI nr
Match: XP_023546101.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546102.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546103.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.4 bits (1435), Expect = 1.1e-154
Identity = 293/436 (67.20%), Postives = 345/436 (79.13%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS+ E EN   +  T+D VV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 139 NSQAEIEN---ETGTSDLVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 198

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DLMAN+ KPRYLQ+FL+LT+L TNTI+E VKT E RAR  YAESI+M+RD
Sbjct: 199 SIGPLHSGRKDLMANSLKPRYLQNFLNLTQLPTNTIVEIVKTWEKRARYCYAESIEMSRD 258

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLI   F  LR+S+ S LWKFWDE+FCDL+LLENQLPFFLLQSLY
Sbjct: 259 EFVELLVFDGCFVVMHLISYSFFELRASDMSNLWKFWDELFCDLILLENQLPFFLLQSLY 318

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNV--SAEICQVNHFVDFVRMHYT 244
           DLCASSQP  + + FIELV QYFIE S+  GL  L+ +V  +    QVNHFVD +R+H+T
Sbjct: 319 DLCASSQPFSKGVRFIELVHQYFIE-SHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFT 378

Query: 245 HTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYD 304
           HT S K+  F  TFWPP AT+LH+CGV FKT K        +AF+DQ GCL+LP+I IYD
Sbjct: 379 HTRSDKMS-FQHTFWPPNATQLHECGVIFKTGK-------GIAFKDQGGCLQLPQINIYD 438

Query: 305 GFETRVRNLIAYEQCH-VGDMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNE 364
            FE RVRNLIAYEQCH   ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NE
Sbjct: 439 DFEKRVRNLIAYEQCHFCSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINE 498

Query: 365 VTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 424
           VTQLFN+L KH+ PG N YN +C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAIL
Sbjct: 499 VTQLFNNLGKHICPGINSYNSDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAIL 558

Query: 425 LLVLTFVQTVVAVVAL 438
           LL LT +QT+VA+V L
Sbjct: 559 LLALTLIQTIVALVDL 562

BLAST of Lag0004629 vs. NCBI nr
Match: XP_022961896.1 (UPF0481 protein At3g47200-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 530.0 bits (1364), Expect = 1.9e-146
Identity = 279/434 (64.29%), Postives = 325/434 (74.88%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS  E EN   +  T DPVV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 139 NSLAEIEN---ETGTFDPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 198

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DL AN+ KP YLQ+FL+LTKL TNTI+E VKT E RAR  YAESI+MNRD
Sbjct: 199 SIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCYAESIEMNRD 258

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLIG  F  LR+S+ S LWKFW E+FCDL+LLENQLPFFLLQSLY
Sbjct: 259 EFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLY 318

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNVSAEICQVNHFVDFVRMHYTHT 244
           DLCASSQP L+ +                               QVNHFVD +R+H+THT
Sbjct: 319 DLCASSQPLLKGV-------------------------------QVNHFVDLLRLHFTHT 378

Query: 245 CSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGF 304
            S +   F  TFWPP AT+LH+CGV FK  K        +AF+DQ GCL+LP+I IYD F
Sbjct: 379 RSDETS-FQHTFWPPNATKLHECGVIFKMGK-------GIAFKDQGGCLQLPQINIYDDF 438

Query: 305 ETRVRNLIAYEQCHVG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVT 364
           E RVRNLIAYEQCH+G ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NEVT
Sbjct: 439 EKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVT 498

Query: 365 QLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLL 424
           QLFN+L KH+ PG N YN++C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAILLL
Sbjct: 499 QLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAILLL 530

Query: 425 VLTFVQTVVAVVAL 438
            LT +QT+VA+V L
Sbjct: 559 ALTLIQTIVAIVDL 530

BLAST of Lag0004629 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.5e-40
Identity = 136/435 (31.26%), Postives = 221/435 (50.80%), Query Frame = 0

Query: 36  SYSSDHVSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDL-MANAHKPRYLQSFLDLTK 95
           S   +   I+RVPE   +  P+AY P ++SIGP+H G + L M   HKPR LQ FLD  K
Sbjct: 40  SAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAK 99

Query: 96  ---LTTNTIIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIM-HLIGSQFLHLR 155
              +  N +++ V  LED+ R+SY+E +K   D  + ++VLDGCF++M  LI S  + L 
Sbjct: 100 KKDVEENVLVKAVVDLEDKIRKSYSEELKTGHD-LMFMMVLDGCFILMVFLIMSGNIELS 159

Query: 156 SSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY---DLCASSQPSLERLSFIELVRQYF 215
                 +      I  DLLLLENQ+PFF+LQ+LY    +  SS   L R++F      +F
Sbjct: 160 EDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSS--DLNRIAF-----HFF 219

Query: 216 IEDSNSEGLDF-LERNVSAEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFWPP------- 275
               + EG  +   RN  A+     H +D +R  +    S   +   P            
Sbjct: 220 KNPIDKEGSYWEKHRNYKAK-----HLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSG 279

Query: 276 --------------TATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGF- 335
                         +A  L   G+ F+  + K+   +++  +     L++P+ + +DGF 
Sbjct: 280 NVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNK--LQIPQ-LRFDGFI 339

Query: 336 ETRVRNLIAYEQCHVGDMSNEVSNFAVFMQYLVQTDQDVKLLIEDG-IIQNNVGSVNEVT 395
            +   N +A+EQ +  D SNE++ + VFM  L+  ++DV  L  D  II+N+ GS NEV+
Sbjct: 340 SSFFLNCVAFEQFYT-DSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVS 399

Query: 396 QLFNSLCKHVA--PGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 437
           + F ++ K V      ++ N   + + E+ K+  +   A  R  +F +PWT  SS A + 
Sbjct: 400 EFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLF 457

BLAST of Lag0004629 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.8e-15
Identity = 107/486 (22.02%), Postives = 193/486 (39.71%), Query Frame = 0

Query: 42  VSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLMANAHKPRYLQSFL--DLTKLTTNT 101
           VSI+ VP+ L  + P++YTP  +SIGP+H  + +L         +   +         + 
Sbjct: 43  VSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHD 102

Query: 102 IIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWK 161
           ++E+++++E + R  Y + I  N +  L ++ +D  F+I  L    F  + +   +R+  
Sbjct: 103 LVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFLKIYSFRKVETL-INRVGH 162

Query: 162 FWDEIFCDLLLLENQLPFF-------------------LLQSLYDLCASSQPSLERLSFI 221
             +EI  D++++ENQ+P F                   LL  L  LC    P + +    
Sbjct: 163 --NEILRDIMMIENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDD 222

Query: 222 ELVRQYFIEDSNSEGLDFLERNVSAEICQVN-------------------HFVDFVRMHY 281
           ++++  F E ++   LDFL + +   I +                      F+D ++  +
Sbjct: 223 QILKAQFQECNHI--LDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKHQF 282

Query: 282 THTCSSK-----LEFFW------PTFWP-------------------------------- 341
               +S+     L F W      P F                                  
Sbjct: 283 KRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIEKP 342

Query: 342 --------PTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRN 401
                   P+ ++LH+ GV FK         V   F   SG   LP I +    ET +RN
Sbjct: 343 PLVEELTIPSVSDLHKAGVRFKPTAHGNISTV--TFDSNSGQFYLPVINLDINTETVLRN 402

Query: 402 LIAYEQCHVGDMSNEVSNFAVFMQY------LVQTDQDVKLLIEDGIIQNNVGSVNEVTQ 430
           L+AYE       +   S   VF +Y      ++ +++DV+LL E G++ + + S  E  +
Sbjct: 403 LVAYE-------ATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAE 462

BLAST of Lag0004629 vs. ExPASy TrEMBL
Match: A0A6J1HBC3 (UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 8.4e-156
Identity = 294/436 (67.43%), Postives = 344/436 (78.90%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS  E EN   +  T DPVV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 40  NSLAEIEN---ETGTFDPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 99

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DL AN+ KP YLQ+FL+LTKL TNTI+E VKT E RAR  YAESI+MNRD
Sbjct: 100 SIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCYAESIEMNRD 159

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLIG  F  LR+S+ S LWKFW E+FCDL+LLENQLPFFLLQSLY
Sbjct: 160 EFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLY 219

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNV--SAEICQVNHFVDFVRMHYT 244
           DLCASSQP L+ + FIELV QYFIE S+  GL  L+ +V  +    QVNHFVD +R+H+T
Sbjct: 220 DLCASSQPLLKGVHFIELVHQYFIE-SHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFT 279

Query: 245 HTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYD 304
           HT S +   F  TFWPP AT+LH+CGV FK  K        +AF+DQ GCL+LP+I IYD
Sbjct: 280 HTRSDETS-FQHTFWPPNATKLHECGVIFKMGK-------GIAFKDQGGCLQLPQINIYD 339

Query: 305 GFETRVRNLIAYEQCHVG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNE 364
            FE RVRNLIAYEQCH+G ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NE
Sbjct: 340 DFEKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINE 399

Query: 365 VTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 424
           VTQLFN+L KH+ PG N YN++C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAIL
Sbjct: 400 VTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAIL 459

Query: 425 LLVLTFVQTVVAVVAL 438
           LL LT +QT+VA+V L
Sbjct: 460 LLALTLIQTIVAIVDL 463

BLAST of Lag0004629 vs. ExPASy TrEMBL
Match: A0A6J1HD53 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 8.4e-156
Identity = 294/436 (67.43%), Postives = 344/436 (78.90%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS  E EN   +  T DPVV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 139 NSLAEIEN---ETGTFDPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 198

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DL AN+ KP YLQ+FL+LTKL TNTI+E VKT E RAR  YAESI+MNRD
Sbjct: 199 SIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCYAESIEMNRD 258

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLIG  F  LR+S+ S LWKFW E+FCDL+LLENQLPFFLLQSLY
Sbjct: 259 EFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLY 318

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNV--SAEICQVNHFVDFVRMHYT 244
           DLCASSQP L+ + FIELV QYFIE S+  GL  L+ +V  +    QVNHFVD +R+H+T
Sbjct: 319 DLCASSQPLLKGVHFIELVHQYFIE-SHKGGLFSLKEHVLLAGIGVQVNHFVDLLRLHFT 378

Query: 245 HTCSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYD 304
           HT S +   F  TFWPP AT+LH+CGV FK  K        +AF+DQ GCL+LP+I IYD
Sbjct: 379 HTRSDETS-FQHTFWPPNATKLHECGVIFKMGK-------GIAFKDQGGCLQLPQINIYD 438

Query: 305 GFETRVRNLIAYEQCHVG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNE 364
            FE RVRNLIAYEQCH+G ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NE
Sbjct: 439 DFEKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINE 498

Query: 365 VTQLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAIL 424
           VTQLFN+L KH+ PG N YN++C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAIL
Sbjct: 499 VTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAIL 558

Query: 425 LLVLTFVQTVVAVVAL 438
           LL LT +QT+VA+V L
Sbjct: 559 LLALTLIQTIVAIVDL 562

BLAST of Lag0004629 vs. ExPASy TrEMBL
Match: A0A6J1HBL7 (UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 9.3e-147
Identity = 279/434 (64.29%), Postives = 325/434 (74.88%), Query Frame = 0

Query: 5   NSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLI 64
           NS  E EN   +  T DPVV SINRIL+Q  S  S   +IY+VPEPLRS KPEAYTPT+I
Sbjct: 139 NSLAEIEN---ETGTFDPVVLSINRILQQAVSSGSSDGTIYKVPEPLRSIKPEAYTPTVI 198

Query: 65  SIGPFHSGREDLMANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRD 124
           SIGP HSGR+DL AN+ KP YLQ+FL+LTKL TNTI+E VKT E RAR  YAESI+MNRD
Sbjct: 199 SIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEKRARYCYAESIEMNRD 258

Query: 125 EFLQLLVLDGCFVIMHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLY 184
           EF++LLV DGCFV+MHLIG  F  LR+S+ S LWKFW E+FCDL+LLENQLPFFLLQSLY
Sbjct: 259 EFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLILLENQLPFFLLQSLY 318

Query: 185 DLCASSQPSLERLSFIELVRQYFIEDSNSEGLDFLERNVSAEICQVNHFVDFVRMHYTHT 244
           DLCASSQP L+ +                               QVNHFVD +R+H+THT
Sbjct: 319 DLCASSQPLLKGV-------------------------------QVNHFVDLLRLHFTHT 378

Query: 245 CSSKLEFFWPTFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGF 304
            S +   F  TFWPP AT+LH+CGV FK  K        +AF+DQ GCL+LP+I IYD F
Sbjct: 379 RSDETS-FQHTFWPPNATKLHECGVIFKMGK-------GIAFKDQGGCLQLPQINIYDDF 438

Query: 305 ETRVRNLIAYEQCHVG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVT 364
           E RVRNLIAYEQCH+G ++ NEVSNFAVFMQ LVQTDQDVKLLIE GII NN GS+NEVT
Sbjct: 439 EKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNNFGSINEVT 498

Query: 365 QLFNSLCKHVAPGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLL 424
           QLFN+L KH+ PG N YN++C+RMK++CKR RHRW++ LRRNYFSTPW CASSIAAILLL
Sbjct: 499 QLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRRNYFSTPWLCASSIAAILLL 530

Query: 425 VLTFVQTVVAVVAL 438
            LT +QT+VA+V L
Sbjct: 559 ALTLIQTIVAIVDL 530

BLAST of Lag0004629 vs. ExPASy TrEMBL
Match: A0A6J1K5T3 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111491939 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 5.8e-125
Identity = 249/422 (59.00%), Postives = 306/422 (72.51%), Query Frame = 0

Query: 19  TTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLMA 78
           T++P V  INRIL+Q  S      +I++VPEPLRS  PEAYTPT+ISIGPFHS R+DL A
Sbjct: 54  TSNPAVVYINRILQQSVSSCFSDDTIFKVPEPLRSVNPEAYTPTVISIGPFHSDRKDLKA 113

Query: 79  NAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVI 138
           N  KP YL+   +L+ L+ NTI+E V+TLE R R  Y ESI+MNRDEF++LLVLD CFV+
Sbjct: 114 NLLKPMYLRHLFNLSHLSVNTIVETVQTLEQRVRCCYTESIEMNRDEFVKLLVLDACFVV 173

Query: 139 MHLIGSQFLHLRSSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLYDLCASSQPSLERLS 198
           MHLI  Q   L   N + LW+ W EIF DL+LLENQLPFFLLQSLYDL A SQP LE  S
Sbjct: 174 MHLISWQNPKLDIPNAAYLWQSWYEIFVDLMLLENQLPFFLLQSLYDLFAPSQPLLEHKS 233

Query: 199 FIELVRQYFIEDSNSEGLDFLERNVSAEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFWP 258
           FI++V+ YF   +NSE L  ++   SAE   VNHFVD VRM  T    ++        WP
Sbjct: 234 FIQIVQDYFSGHNNSE-LILIDIESSAE--NVNHFVDLVRMRKTDMVFNR-TIIQGLVWP 293

Query: 259 PTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAYEQCH 318
           P+ATELH+CGV F T        +D+ F DQSGCL+L  I I + FE RV+N+IAYEQ H
Sbjct: 294 PSATELHECGVIFST-------GIDIKFNDQSGCLQLLPINIDNTFEKRVKNIIAYEQHH 353

Query: 319 VG-DMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVAPGN 378
           +  ++ NEVSNFA+FM  LVQTDQDVKLLIE GIIQN +GS+ +VTQLF +L K++  G 
Sbjct: 354 IHMNIWNEVSNFALFMTSLVQTDQDVKLLIEGGIIQNELGSIKDVTQLFYNLGKYINIGF 413

Query: 379 NFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQTVVAVVAL 438
           N YN +CQ+MK++CKRRRHRW+ SLRRNYFSTPW CAS+IAAILLL LT +QT+VAV+  
Sbjct: 414 NSYNSDCQKMKDYCKRRRHRWMTSLRRNYFSTPWLCASTIAAILLLALTLIQTIVAVLTG 464

Query: 439 YK 440
           +K
Sbjct: 474 FK 464

BLAST of Lag0004629 vs. ExPASy TrEMBL
Match: A0A6J1HD16 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111462969 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.0e-121
Identity = 237/410 (57.80%), Postives = 294/410 (71.71%), Query Frame = 0

Query: 31  LRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLMANAHKPRYLQSFL 90
           + QV   +     IY+VPEPLRS   EAYTPT ISIGP HSGR+DL AN+ KP YL+ FL
Sbjct: 12  INQVEIENETEGIIYKVPEPLRSMNSEAYTPTAISIGPLHSGRKDLKANSLKPIYLRHFL 71

Query: 91  DLTKLTTNTIIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIMHLIGSQFLHLR 150
           DL +L+ NTI+E V+TLE RAR  Y E I+M+RDEF++LLV D CFV+MHL   Q+ +L 
Sbjct: 72  DLAQLSVNTIVETVQTLEQRARYCYTEFIEMSRDEFVELLVFDACFVVMHLTSWQYPNLD 131

Query: 151 SSNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLYDLCASSQPSLERLSFIELVRQYFIED 210
           + N   LW+ W+EI  DL+LLENQLPFFLLQSLYDL A SQP LE  SFI++V+ YF E 
Sbjct: 132 TPNAVNLWQSWNEISHDLMLLENQLPFFLLQSLYDLFAPSQPLLEHKSFIQIVQYYFSEH 191

Query: 211 SNSEGLDFLERNVSAEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFWPPTATELHQCGVY 270
            N + L F++  +   +  V HFVD V M  T   S + +      WP  ATELH+CGV 
Sbjct: 192 KNPK-LFFIDIKL-LSVDDVKHFVDLVTMPNTRMYSHE-KSGQRLLWPLNATELHECGVI 251

Query: 271 FKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAYEQCHVG-DMSNEVSNF 330
           F++          + F DQ GCL+LP I IYD FE RVRNLIAYEQCH+G ++ N VSNF
Sbjct: 252 FRS-------GDGIEFNDQGGCLQLPPIKIYDDFEKRVRNLIAYEQCHIGNELRNRVSNF 311

Query: 331 AVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVAPGNNFYNYECQRMKE 390
           AVFMQ LVQ+DQDVKLLIE GII+N +G +NE+TQLFN+L KH+  G N+YN +C++MK+
Sbjct: 312 AVFMQCLVQSDQDVKLLIEGGIIENYLGGINEITQLFNNLGKHIFIGINYYNSDCKKMKD 371

Query: 391 FCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQTVVAVVALYK 440
           +C RR HRW+ SLRRNYFSTPW CASSIAAILLL LT +QT++AV   +K
Sbjct: 372 YCMRRHHRWMTSLRRNYFSTPWLCASSIAAILLLALTLIQTILAVFTRFK 411

BLAST of Lag0004629 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 223.8 bits (569), Expect = 2.7e-58
Identity = 152/424 (35.85%), Postives = 235/424 (55.42%), Query Frame = 0

Query: 21  DPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLMA-N 80
           D +V SI   L  + S S+    IY+VP  LR   P+AYTP L+S GP H G+E+L A  
Sbjct: 273 DALVDSIKAKLAFLSSLST-KCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAME 332

Query: 81  AHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIM 140
             K RYL SF+  T  +   ++   +T E  AR  YAE +K++ DEF+++LV+DG F++ 
Sbjct: 333 DQKYRYLLSFIPRTNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVE 392

Query: 141 HLIGSQFLHLRSSNCSRLW---KFWDEIFCDLLLLENQLPFFLLQSLYDLCASSQPSLER 200
            L+ S +  LR  N  R++       ++  D++L+ENQLPFF+++ ++ L   +      
Sbjct: 393 LLLRSHYPRLRGEN-DRIFGNSMMITDVCRDMILIENQLPFFVVKEIF-LLLLNYYQQGT 452

Query: 201 LSFIELVRQYFIEDSNSEGLDFLER-NVSAEICQVNHFVDFVRMHYTHTCSSKLEFFW-P 260
            S I+L +++F          FL R +    I +  HFVD +R  Y      KLE+    
Sbjct: 453 PSIIQLAQRHFSY--------FLSRIDDEKFITEPEHFVDLLRSCYLPQFPIKLEYTTVK 512

Query: 261 TFWPPTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAY 320
               P ATELH  GV FK   E   C +D++F D  G L++P I++ D  E+  +N+I +
Sbjct: 513 VDNAPEATELHTAGVRFK-PAETSSCLLDISFAD--GVLKIPTIVVDDLTESLYKNIIGF 572

Query: 321 EQCHVGDMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVA 380
           EQC     +    ++ + +   +++  D  LLI  GII N +G+  +V+ LFNS+ K V 
Sbjct: 573 EQCRCS--NKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNSVDVSNLFNSISKEVI 632

Query: 381 PGNNFY-NYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQTVVA 438
               FY +   + ++ +C    +RW A LRR+YF  PW  AS  AA+LLL+LTF+Q+V +
Sbjct: 633 YDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVCS 680

BLAST of Lag0004629 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 189.1 bits (479), Expect = 7.5e-48
Identity = 141/462 (30.52%), Postives = 240/462 (51.95%), Query Frame = 0

Query: 1   MELQNSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYT 60
           ++ QN +PE+      I   D     + + LR+  + S D + IYRVP+ L+    ++Y 
Sbjct: 102 LQKQNQKPEETREEWVISIRD----KMEQALREDATTSWDKLCIYRVPQYLQENNKKSYF 161

Query: 61  PTLISIGPFHSGREDLM-ANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESI 120
           P  +S+GPFH G + L+  + HK R +   +  TK      I+ +K LEDRAR  Y   I
Sbjct: 162 PQTVSLGPFHHGNKHLLPMDRHKWRAVNMVMARTKHDIEMYIDAMKELEDRARACYEGPI 221

Query: 121 KMNRDEFLQLLVLDGCFVIMHLIG-----SQFLHLRSSNCSRLWKFWDEIFCDLLLLENQ 180
            ++ ++F ++LVLDGCFV+    G     S+  + R+     +      I  D+++LENQ
Sbjct: 222 DLSSNKFSEMLVLDGCFVLELFRGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLENQ 281

Query: 181 LPFFLLQSLYDLCASSQPSLERLSFIELVRQYF-----IEDSNSEGLDFLERN-----VS 240
           LP F+L  L ++    +     +S   L  ++F      ++  ++  D LE++     ++
Sbjct: 282 LPLFVLNRLLEIQLGKRHQTGLVS--RLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIA 341

Query: 241 AEICQVNHFVDFVRMHYTHTCS------SKLEFFWPTFWPP--------TATELHQCGVY 300
            +     H +D  R +    CS      S++ + W T              TEL + G+ 
Sbjct: 342 DKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVADKRQQQLIHCVTELREAGIK 401

Query: 301 FKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAYEQCHVGDMSNEVSNFA 360
           F+T K  +   +    R ++G LE+PK++I+DG ++   NLIA+EQCH+ D SN+++++ 
Sbjct: 402 FRTRKTDRFWDI----RFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHI-DSSNDITSYI 461

Query: 361 VFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVA--PGNNFYNYECQRMK 420
           +FM  L+ + +DV+ L   GII++ +G+  EV  LFN LC+ VA  P N++ +    ++ 
Sbjct: 462 IFMDNLIDSSEDVRYLHYCGIIEHWLGNDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVD 521

Query: 421 EFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQT 431
               R+ +   A L+  YF+ PW   S  AA++LLVLT  Q+
Sbjct: 522 RNYSRKWNVLKAILKHKYFNNPWAYFSFFAALVLLVLTLFQS 552

BLAST of Lag0004629 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 179.9 bits (455), Expect = 4.5e-45
Identity = 142/471 (30.15%), Postives = 232/471 (49.26%), Query Frame = 0

Query: 1   MELQNSEPEKENGRIQIVTTDPVVASINRILRQVPSYSSDHVSIYRVPEPLRSTKPEAYT 60
           ++ +N +PE+      I   D     + +++R   + S D + IYRVP  L+ +   +Y 
Sbjct: 76  LQTRNQQPEETREEWVIWIKD----KMEQVMRDAATTSWDKICIYRVPLSLKKSDKNSYF 135

Query: 61  PTLISIGPFHSGREDLM-ANAHKPRYLQSFLDLTKLTTNTIIERVKTLEDRARRSYAESI 120
           P  +S+GP+H G E L   + HK R +   +  TK      I+ +K LE+RAR  Y   I
Sbjct: 136 PQAVSLGPYHHGDEHLRPMDYHKWRAVNMVMKRTKQGIEMYIDAMKELEERARACYEGPI 195

Query: 121 KMNRDEFLQLLVLDGCFVIMHLIG-----SQFLHLRSSNCSRLWKFWDEIFCDLLLLENQ 180
            ++ ++F Q+LVLDGCFV+    G     S+  + R+     +      I  D+L+LENQ
Sbjct: 196 GLSSNKFTQMLVLDGCFVLDLFRGAYEGFSKLGYDRNDPVFAMRGSMHSIRRDMLMLENQ 255

Query: 181 LPFFLLQSLYDLCASSQ------PSLERLSFIELVRQYF----IEDSNSEGLDFLERNVS 240
           LP F+L  L +L   +Q        L    F  L+  Y     IE+S      F      
Sbjct: 256 LPLFVLNRLLELQLGTQYQTGLVAQLAVRFFNPLMPTYMSSTKIENSQENNNKFFNPIAD 315

Query: 241 AEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFW---PPTA-----------TELHQCGVY 300
            E  ++ H +D  R                + W   P  A           TEL + G+ 
Sbjct: 316 KEKEEL-HCLDVFRRSLLQPSLKPDPRLSRSRWSRKPLVADKRQQQLLHCVTELREAGIK 375

Query: 301 FKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAYEQCHVGDMSNEVSNFA 360
           FK  K  +    D+ F+  +GCLE+PK++I+DG ++   NLIAYEQCH+ D +N+++++ 
Sbjct: 376 FKRRKSDR--FWDIQFK--NGCLEIPKLLIHDGTKSLFSNLIAYEQCHI-DSTNDITSYI 435

Query: 361 VFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVA--PGNNFYNYECQRMK 420
           +FM  L+ + +D++ L    II++ +G+ +EV  +FN LC+ VA    N + +    ++ 
Sbjct: 436 IFMDNLIDSAEDIRYLHYYDIIEHWLGNDSEVADVFNRLCQEVAFDLENTYLSELSNKVD 495

Query: 421 EFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQTVVAVVALYK 440
            +  R+ +   A+L+  YFS PW   S  AA++LL+LT  Q+       +K
Sbjct: 496 RYYNRKWNVLKATLKHKYFSNPWAYFSFFAAVILLLLTLFQSFFTSYPYFK 536

BLAST of Lag0004629 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 173.7 bits (439), Expect = 3.2e-43
Identity = 128/432 (29.63%), Postives = 219/432 (50.69%), Query Frame = 0

Query: 44  IYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLMA-NAHKPRYLQSFLDLTKLTTNTIIE 103
           IYRVP  L+    ++Y P  +S+GP+H G++ L + + HK R +   L  T       I+
Sbjct: 105 IYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYID 164

Query: 104 RVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIMHLIG-----SQFLHLRSSNCSRL 163
            ++ LE++AR  Y   + ++ +EF+++LVLDGCFV+    G     ++  + R+     +
Sbjct: 165 AMRELEEKARACYEGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAM 224

Query: 164 WKFWDEIFCDLLLLENQLPFFLLQSLYDLCASSQPSLERLSFIELVRQYF------IEDS 223
                 I  D+++LENQLP F+L  L +L   ++     ++  +L  ++F       E  
Sbjct: 225 RGSMHSIQRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVA--QLAIRFFDPLMPTDEPL 284

Query: 224 NSEGLDFLERNVSAEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFWP------------- 283
              G   LE +++ +    + F D   +H        L    P   P             
Sbjct: 285 TKSGQSKLENSLARD-KSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRV 344

Query: 284 ---------PTATELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVR 343
                       TEL + G+ F+  K  +    D+ F+  +G LE+P+++I+DG ++   
Sbjct: 345 ADKRRQQLIHCVTELKEAGIKFRRRKTDR--FWDMQFK--NGYLEIPRLLIHDGTKSLFL 404

Query: 344 NLIAYEQCHVGDMSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSL 403
           NLIA+EQCH+ D SN+++++ +FM  L+ + +DV  L   GII++ +GS +EV  LFN L
Sbjct: 405 NLIAFEQCHI-DSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVADLFNRL 464

Query: 404 CKHVA--PGNNFYNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTF 440
           C+ V     +++ +     +  +   + + W A+L+  YF+ PW   S  AA++LLVLTF
Sbjct: 465 CQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTF 524

BLAST of Lag0004629 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 173.3 bits (438), Expect = 4.2e-43
Identity = 130/420 (30.95%), Postives = 213/420 (50.71%), Query Frame = 0

Query: 38  SSDHVSIYRVPEPLRSTKPEAYTPTLISIGPFHSGREDLM-ANAHKPRYLQSFLDLTKLT 97
           S D++ IYRVP  L+    ++Y P ++SIGP+H G + LM    HK R +   +   K  
Sbjct: 100 SWDNLCIYRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHD 159

Query: 98  TNTIIERVKTLEDRARRSYAESIKMNRDEFLQLLVLDGCFVIMHLIGS-----QFLHLRS 157
               I+ +K LE++AR  Y   I MNR+EF+++LVLDG F+I    G+     +  +  +
Sbjct: 160 IEMYIDAMKELEEKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIGYAPN 219

Query: 158 SNCSRLWKFWDEIFCDLLLLENQLPFFLLQSLYDLCASSQPSLERLSFIELVRQYF---- 217
                +      I  D+++LENQLP+ +L+ L  L    +P +     ++L + +F    
Sbjct: 220 DPVFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQL---QRPDVLDKVNVQLFQPFFQPLL 279

Query: 218 ------IEDSNSEGLDFLERNVSAEICQVNHFVDFVRMHYTHTCSSKLEFFWPTFWPPTA 277
                  E+     LD L R +       +  +  V                P       
Sbjct: 280 PTREVLTEEGGLHCLDVLRRGLLQSSGTSDEDMSMVNKQ-------------PQQLIHCV 339

Query: 278 TELHQCGVYFKTEKEKKKCAVDLAFRDQSGCLELPKIIIYDGFETRVRNLIAYEQCHVGD 337
           TEL   GV F   +++     D+ F+  +G L++PK++I+DG ++   NLIA+EQCH+  
Sbjct: 340 TELRNAGVEFM--RKETGHFWDIEFK--NGYLKIPKLLIHDGTKSLFLNLIAFEQCHI-K 399

Query: 338 MSNEVSNFAVFMQYLVQTDQDVKLLIEDGIIQNNVGSVNEVTQLFNSLCKHVA--PGNNF 397
            S +++++ +FM  L+ + +DV  L   GII+N +GS +EV+ LFN L K V   P + +
Sbjct: 400 SSKKITSYIIFMDNLINSSEDVSYLHHYGIIENWLGSDSEVSDLFNGLGKEVIFDPNDGY 459

Query: 398 YNYECQRMKEFCKRRRHRWLASLRRNYFSTPWTCASSIAAILLLVLTFVQTVVAVVALYK 440
            +     +  + +R+ +   A+LR  YF+ PW   S IAA+ LL+ TF Q+  AV A +K
Sbjct: 460 LSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFAVFAYFK 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022961897.11.7e-15567.43UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 U... [more]
XP_022961893.11.7e-15567.43UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961894.1 U... [more]
XP_023546104.11.1e-15467.20UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_02354... [more]
XP_023546101.11.1e-15467.20UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02354... [more]
XP_022961896.11.9e-14664.29UPF0481 protein At3g47200-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SD532.5e-4031.26UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8974.8e-1522.02Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HBC38.4e-15667.43UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HD538.4e-15667.43UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HBL79.3e-14764.29UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1K5T35.8e-12559.00UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111491939 PE=4 ... [more]
A0A6J1HD161.0e-12157.80UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111462969 PE=... [more]
Match NameE-valueIdentityDescription
AT4G31980.12.7e-5835.85unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50130.17.5e-4830.52Plant protein of unknown function (DUF247) [more]
AT3G50140.14.5e-4530.15Plant protein of unknown function (DUF247) [more]
AT3G50120.13.2e-4329.63Plant protein of unknown function (DUF247) [more]
AT3G50160.14.2e-4330.95Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 44..425
e-value: 3.2E-101
score: 339.5
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 20..436
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 20..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004629.1Lag0004629.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane