Lag0004473 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004473
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like superfamily protein
Locationchr6: 4233174 .. 4239266 (-)
RNA-Seq ExpressionLag0004473
SyntenyLag0004473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACGATTCCTTCCTTCAGATTCGAGTGTTGAAGTTGAAGAAGAAGAAGAAGAAACAGAGGCGAAGAACAGAGGCGGTGGCTCGGCTCACTCCGTCCGTGGCCGTGGATTGTAGAGGAGAGAGAGGCTTTTCACGAAGACAACTGCCAAAATATAGTCTTCTCTCCTTCCATTTTTTACCCTTTTAATTGTTATTATTATTATTATTATTTTTAAATGGTTTATTGAAAATTTTTATTGATGTTCAGGTCAAAATTTTATTTCATCTTGAATATAAGAGAGAGAAATTAAAATTTTCATTTTAAATTTAAATGAAATAGTGATGGGGAATAAAATAGATTTTGATTGAACGGTTAATAAAATTAGATATATTGAATCATTTTTACTACATAGTTTAATTGCTTAAGACATGCGTCATTAATTAGGAGGTCTTCAAATTTCCACCCTCACATGTTAAAATAAGAGCGGAGGTATCTAACGGTTAGTAAAGATAGACCGATTGAATGATTTGTTATTTTTATTGTTTATTTTGTCAAATATGATTATTCTAATTATTTTTCCAAAAAAAATATCTAATAGATAAATTCTAATTTAGTATGGGAAATTTTATAATTGTTTTAGTTTGCTACCACTAGCTAAATTTTGCATAAAATTCTATTTTATTTACTATTCCATTATTCGATTAACAATCAAGAGGGAATCTCTCGGGCCAAATATTGTTGAACTAAAAAAAATCATTTGATTTTGTTATGAGATTTGATGTTTGAGCTATTTATGTGGCTAAAAATGAGTAGACATTATTTATTTATTTTATTATTATTATTATTTTTAGGAGAAAAAATGAGTAGACATTTGATATTCAATTAAATGAATAAAAGTATATTTAAATTTAGTTTCTATTGGATAAATTATTTAAAAACACTAAAATGATTTACGTAAAATTTAGAGAGTAAAATAAAAAACATTCAAAAGATTAAAAACAAAAATGAAATAAATATAAAACTTCAATAGAATAATGCTTGATTAACATAATTAAACATGATTTAAATAATTTTAGAGTCATAATTTTGTGCAATATTCTAAAAACGCACAGGTATAAACATTTTTGTAAATATAAGAATTAAGAAAAACTATCAATTTGTAAATATAAAAAGGGAAATTGAAAGAGAAAACTATCAATTTGTTTGTTTTTTTTTTTTTTTTTTTGGGAATATTGAGAAAAATTAATTAGGACAGTGTTCGGCTTGTTTTGATTAGAAGAAAAATGAAAAATGAAAAATTAAAAAAATGACGTGCTCAAATGTAGCTGAGAATACGATCTTCGTGGGTTTTATTTTATGATTTTAATACAAGTCAACGAATATACACAAAACACTAAACAGTATGATTGGAGAGCTTATTATAAACTTGCGAATATAAAATATTGCAAATTTGCGTTTACTTTTCTTTTCTTGGTTTAATTACAAAATTAAAATATCGCCAAATTACAATATTAGTTAATGTGGCCCATGTAAAAGGTTGTACTGTTTATCAAGTCAATGTATCAATTTCATAAAACGATGCGATTATTAAATTACATTTTTAATCCTAGTGCTATAATTTTAATTTAGTTTACATGTTTATCTTATTATCATCTCTGTTTTTATTATTTAAAGTTTACTAAATATCAGTATCATTGATAATTAATGAGTAAAAATACCAAACGCGACAAATTATTTATGAATATATAAAATAAATTTATGATGTGATTTTTGTTTTAATAACTAAGAAGTGTGACAAGTTGCATGAATTCTCCACAAAATAAAAATTATAAAAAAGAAACTATTATATTAGACTTTCGGACTTTATTTTGGTTGAAAAAACATAGAAATTAAATGAAAGCTTCATATCAATTAAATAATGAAAATATCATGAATTTATAAACAATGACATGACATAAGATATTTCGAGTGAAACAAAAAACAAAGTCGTTAAAGTTTATTTTCGAAATGAACAATATCATAATCATTATATAAATGAATATAATGAAAATAAATAGTTAACATGTCTTCTTTTGAGTTACAAATTTCACCGATTTACTACACTTATATTTTGAAAGATAGAGACGAATAATGAACTTTTTAAAATAAGAAGAATTAAATTGAAATTAAAATTCAAGGCTAAATATGTAAATTTATAGTACAACCGACGTATTTCAATTAATTTAACTTAAAAAAATATATTTCAATTAATTAAAAATTTTAAAACGCTATGGTATTGGTATTTCTATTTCATTTCTGTCCGCATCATGATCCACATCTTCTCAAGGTATCGCATGGATTAGCACATGATGTTGGTATCTATTTCATTTATATCAAATAAAGGGCAAAAGGGTAAAATAACATTCACATGTACACAAATACATTATACTTTAAACCTATAAAATATAAATGAAAGTTGTTAATATATATATATATATATATATATAAATGAAAGTTCTTTTGCCAACAAAAACATAATTCAATTTAATCCAAAAAAAAAACATAATTCAATTGATATAAAACTTGTATCGATCAATAGGTCAATATTCAAATCTCTCACTCAAAATAACAAGCTCATGAACATATAAAAAGTTTTTTAAATTTAAAAAATTTAATAGACATAAACATGAAAGTTGGAAACCAAACTTCTAATTTAACTAAATAATTTTCACCATTTCTATTATTTCTTTTTTTTTTCTTTTTGACAATATGTATGGTGGGGATTCGAACCATAGATCTCGTTGTCATGATCAATACAACTTTATGTCAGTTGAGCTATGTTGCTGTTCGTTTCATCGTTTCTAAATTAACACCTGCAAAAACTTCATTATTAGATCCCTAAAATAATGTAGAAATTAAACAACCATTAATTCTACTAGTAATTTTACCGCATGGAAATTTATAATTCAAATTTGATTATTTTAAAGGACAAATAGAGTGGAAGGCCAGCCACGTGACATGTTGCCAACATGGCACCAAGTTAGAACCATGTTAACTTCATTTACTTGTCATTACACACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTGCGTGTGAAAGATCTAGCTTACGAGTATATTAAAATATTCGTAGCTATTCTAACAAAATAGAGCTATTGATTTTCAAAAAAAAAAAAATAGAGCTATTAATTTTTCAAAAAAAAAAAAATAGAGCTATTAAAAATAAGGTCGTACAAGCCAATAAAATATTTTTTTCTCAAAATGTTTTCATATCCAATTAGAAAACGGGTAGAGAAAAAATTCGATCCATCAATAGTGATGATTTTCGAATATGATACACTCAAGTTTAAGTAAACTAATAGAAACTTTAAGGTCTTCTAGAACCTTTTTTTTTCCGAAAAGAAGTTTATTATTAATGATATTTTTGTAATGGTTAAGACATTTACTTATTATCTTGTAAGGTTGAAAGTTTAAATCTCCATCATCTTCACATTTATTGTAATTAAAAAAAAAAGTTGTATAATCTTTGTTTAATTATTTGAAATTAGCCCAAAGTTTGGAAACGAAAAATATGTTTCACAATGATTAGTGTTTTAATAAAGTTATTTTCTTGCCCTTATTTAGGTGAAATAAACTAGCTTTTATAATGGTTTTACCAAATAGACGCCAATCTTCTAGATGATTTTAAATTTGGTTAATAATCTTTTAAAACTCTGAAATTTATACCCAACATATTTTTTAGAAAAATAGTTCAATAATATATAGTCATGAGAATTTGAAAACTTCTAACCTTTATACCATGACTAATAGTATAGTAGAGCTATGTTTAGATTTATATTATTAATATAAAGTTGATATGTCATCAAGTTATTGTTTTAATAACAATACTCATATATATGCTTACTTGAGGAAGAATTCAACTAATTAATGCATATATTATATACTAAATGGTCAAAAGTTACCAATCTAAACATATATAGTTTAGTGGATTAAGACACATGTCCTCAATCAAGAGATTAGAAGTTCAAATCTCTCTACCTTTGCGGATGATAGTCTTATTTTTTGTCAAGCATCTGTGGGTCAGGTGACAGCGCTTCATTCTCTTCTTAAAAAGTATGAAATAGCCTCAGGACAAGTCATTAATGTGGAAAAGTCTGCAATGTTCTTCTCACCGAACGTGCCAGCGGAACTTCGTGTATCTCTGAAACAAATCATGGGCATGCCGATTGTTGATAATCTGGGCAGATACTTAGGCGTGCCGTCGTCTTTTTCTAGAAATAGGAAAGATGATTTCAAGGGGGTCAAACAACGGGTTTGGCAAACCTTACAGGGTTGGAAAGGTAAATTTTTCTCCATGGGTGGAAAAGAGGTGCTCATAAAAAGTGTGGCACAAGCTATTCCTACTTTCATAATGAGTTGTTTCAGACTCCCGGTTACTCTTTGCGATGACCTGCATAAAATGATGGCTAAATTCTGGTGGGGCTCTACGGAGGAGAAAAAGAAAATTCATTGGAAGCGCTGGGATTTTATGTGTAAACCAAAAGAGTTGGGCGGGTTAAATTTCCGGGACTTGAAAGTCTTCAATAAGGCACTGTTAGCAAAACAGGTTTGGCGAATTTTTACAAATCCGGGTCTCCTAGTATCCAAAGTCATCCATGGAAGATATGCCCATAATATGTCTGTTTTATCTGCCCCTGTTAAATCCAACTGCTCGGTTTTCTGGAGAGGATTTGTATGGGCTCGTGGCCTACTTTCCCACGGGATGCGTAAACAGGTTGGCAATGGAGCATCTATCAAGTTCTTCCAAGACCCGTGGATTCCTAAGGAGACTAGTTTTAAACCTCTACGAATTGACATGTCCAATATCAATGACGATCCTTGGGTCGCGGATTTTATAACTCCATCCAAAGGATGGAATGTTGCGAAAATAGAAAATGTCGTAACCAAGGAAGATGCTAATGTAATTGCGGCTATCCCTATCAGTGTTGTTAATGAGGAAGATAGATGGGTTTGGCATTACTCCAAGAATGGAAATTATACGGTCAAAAGTGGTTATAAACAGGCAATTATGGCAACAGTTGATACCGTGTCAACATCTTCCAATTACACCAAACATTGGTGGGGTGCTCTATGGAACTCAAAACTTCCTACCAAGATTAAAGTTTTCATCTGGAAAGCATTCCATGAATGCTTACCAACAGCTTATAGCCTTTGGAGAAGAAGAATGGATGTATCTCCAAAATGCGGCATGTGCAATAAGAAGAACGAAACAGTTGATCATGCGCTTTGTGGATGCAAGAGAGCAAAGATGATTTGTGACCACGTTTTTCCACGAGTAGATGATCATATCCCCGTGGAGAATAATTTTGCCGATCGCTTAATTCACCTTGCAAGGCAGCTGAAGGAAGCAGACTTCGAAAAGGCTTGTATTGCGTTTTGGTCCGTATGGAACAATAGAAACACACGGCTTTTGGGCAGTCCGATTGTTGAATGGACACATCAGTGTGAGTGGGTTGTTAACTATTGGGCAGAAATTTCAAGGGGGAAACAGGAGGTCGTGTCGGACCATACCCATTTGAATACATGTGCACTGGAAGCTGACGGGGCTGTTACTCTCTTTGCAGACGCGGCCGTCCTCCCGACCTTAGATGGAGCTGGCCTAGGTGTCTTATTGGTTAATGAAGTCGGCAATATTTGTGGGGCAATGGAGATATATGAGAGTTCAGCTTTGTCTCCGTTCGCAGCGGAAGTTCAGGCTATAATGCATGGACTAAGATTGACTGCACGCATGGAAATACAAAGAGTTCATGTGTACTCGGACTCTTTAAATGCAATTCGGATGATCAATGGAGAGATTGGAGAGTGGCTGGAAGTTCGACACTGGATATCAGAACTTCATGATCTATGCAAAGGGTTCCTTTCAGTGTGTTTCTCTTATATCCCAAGGGATAAAAATAGGAAAGCAGATGCTCTAGCGAAGGATGCGTTAATGCACCAGCAAAATATGTTGTGGGTAGAAAATTTTCCGCTATGGCTATTATCAATGGGTAACCAATCCCACGATAAGAGTATTGTAAGTTGTGTTCGTCTCTAA

mRNA sequence

ATGAAACGATTCCTTCCTTCAGATTCGAGTGTTGAAGTTGAAGAAGAAGAAGAAGAAACAGAGGCGAAGAACAGAGGCGGTGGCTCGGCTCACTCCGTGACAGCGCTTCATTCTCTTCTTAAAAAGTATGAAATAGCCTCAGGACAAGTCATTAATGTGGAAAAGTCTGCAATGTTCTTCTCACCGAACGTGCCAGCGGAACTTCGTGTATCTCTGAAACAAATCATGGGCATGCCGATTGTTGATAATCTGGGCAGATACTTAGGCGTGCCGTCGTCTTTTTCTAGAAATAGGAAAGATGATTTCAAGGGGGTCAAACAACGGGTTTGGCAAACCTTACAGGGTTGGAAAGGTAAATTTTTCTCCATGGGTGGAAAAGAGGTGCTCATAAAAAGTGTGGCACAAGCTATTCCTACTTTCATAATGAGTTGTTTCAGACTCCCGGTTACTCTTTGCGATGACCTGCATAAAATGATGGCTAAATTCTGGTGGGGCTCTACGGAGGAGAAAAAGAAAATTCATTGGAAGCGCTGGGATTTTATGTGTAAACCAAAAGAGTTGGGCGGGTTAAATTTCCGGGACTTGAAAGTCTTCAATAAGGCACTGTTAGCAAAACAGGTTTGGCGAATTTTTACAAATCCGGGTCTCCTAGTATCCAAAGTCATCCATGGAAGATATGCCCATAATATGTCTGTTTTATCTGCCCCTGTTAAATCCAACTGCTCGGTTTTCTGGAGAGGATTTGTATGGGCTCGTGGCCTACTTTCCCACGGGATGCGTAAACAGGTTGGCAATGGAGCATCTATCAAGTTCTTCCAAGACCCGTGGATTCCTAAGGAGACTAGTTTTAAACCTCTACGAATTGACATGTCCAATATCAATGACGATCCTTGGGTCGCGGATTTTATAACTCCATCCAAAGGATGGAATGTTGCGAAAATAGAAAATGTCGTAACCAAGGAAGATGCTAATGTAATTGCGGCTATCCCTATCAGTGTTGTTAATGAGGAAGATAGATGGGTTTGGCATTACTCCAAGAATGGAAATTATACGGTCAAAAGTGGTTATAAACAGGCAATTATGGCAACAGTTGATACCGTGTCAACATCTTCCAATTACACCAAACATTGGTGGGGTGCTCTATGGAACTCAAAACTTCCTACCAAGATTAAAGTTTTCATCTGGAAAGCATTCCATGAATGCTTACCAACAGCTTATAGCCTTTGGAGAAGAAGAATGGATGTATCTCCAAAATGCGGCATGTGCAATAAGAAGAACGAAACAGTTGATCATGCGCTTTGTGGATGCAAGAGAGCAAAGATGATTTGTGACCACGTTTTTCCACGAGTAGATGATCATATCCCCGTGGAGAATAATTTTGCCGATCGCTTAATTCACCTTGCAAGGCAGCTGAAGGAAGCAGACTTCGAAAAGGCTTGTATTGCGTTTTGGTCCGTATGGAACAATAGAAACACACGGCTTTTGGGCAGTCCGATTGTTGAATGGACACATCAGTGTGAGTGGGTTGTTAACTATTGGGCAGAAATTTCAAGGGGGAAACAGGAGGTCGTGTCGGACCATACCCATTTGAATACATGTGCACTGGAAGCTGACGGGGCTGTTACTCTCTTTGCAGACGCGGCCGTCCTCCCGACCTTAGATGGAGCTGGCCTAGGTGTCTTATTGGTTAATGAAGTCGGCAATATTTGTGGGGCAATGGAGATATATGAGAGTTCAGCTTTGTCTCCGTTCGCAGCGGAAGTTCAGGCTATAATGCATGGACTAAGATTGACTGCACGCATGGAAATACAAAGAGTTCATGTGTACTCGGACTCTTTAAATGCAATTCGGATGATCAATGGAGAGATTGGAGAGTGGCTGGAAGTTCGACACTGGATATCAGAACTTCATGATCTATGCAAAGGGTTCCTTTCAGTGTGTTTCTCTTATATCCCAAGGGATAAAAATAGGAAAGCAGATGCTCTAGCGAAGGATGCGTTAATGCACCAGCAAAATATGTTGTGGGTAGAAAATTTTCCGCTATGGCTATTATCAATGGGTAACCAATCCCACGATAAGAGTATTGTAAGTTGTGTTCGTCTCTAA

Coding sequence (CDS)

ATGAAACGATTCCTTCCTTCAGATTCGAGTGTTGAAGTTGAAGAAGAAGAAGAAGAAACAGAGGCGAAGAACAGAGGCGGTGGCTCGGCTCACTCCGTGACAGCGCTTCATTCTCTTCTTAAAAAGTATGAAATAGCCTCAGGACAAGTCATTAATGTGGAAAAGTCTGCAATGTTCTTCTCACCGAACGTGCCAGCGGAACTTCGTGTATCTCTGAAACAAATCATGGGCATGCCGATTGTTGATAATCTGGGCAGATACTTAGGCGTGCCGTCGTCTTTTTCTAGAAATAGGAAAGATGATTTCAAGGGGGTCAAACAACGGGTTTGGCAAACCTTACAGGGTTGGAAAGGTAAATTTTTCTCCATGGGTGGAAAAGAGGTGCTCATAAAAAGTGTGGCACAAGCTATTCCTACTTTCATAATGAGTTGTTTCAGACTCCCGGTTACTCTTTGCGATGACCTGCATAAAATGATGGCTAAATTCTGGTGGGGCTCTACGGAGGAGAAAAAGAAAATTCATTGGAAGCGCTGGGATTTTATGTGTAAACCAAAAGAGTTGGGCGGGTTAAATTTCCGGGACTTGAAAGTCTTCAATAAGGCACTGTTAGCAAAACAGGTTTGGCGAATTTTTACAAATCCGGGTCTCCTAGTATCCAAAGTCATCCATGGAAGATATGCCCATAATATGTCTGTTTTATCTGCCCCTGTTAAATCCAACTGCTCGGTTTTCTGGAGAGGATTTGTATGGGCTCGTGGCCTACTTTCCCACGGGATGCGTAAACAGGTTGGCAATGGAGCATCTATCAAGTTCTTCCAAGACCCGTGGATTCCTAAGGAGACTAGTTTTAAACCTCTACGAATTGACATGTCCAATATCAATGACGATCCTTGGGTCGCGGATTTTATAACTCCATCCAAAGGATGGAATGTTGCGAAAATAGAAAATGTCGTAACCAAGGAAGATGCTAATGTAATTGCGGCTATCCCTATCAGTGTTGTTAATGAGGAAGATAGATGGGTTTGGCATTACTCCAAGAATGGAAATTATACGGTCAAAAGTGGTTATAAACAGGCAATTATGGCAACAGTTGATACCGTGTCAACATCTTCCAATTACACCAAACATTGGTGGGGTGCTCTATGGAACTCAAAACTTCCTACCAAGATTAAAGTTTTCATCTGGAAAGCATTCCATGAATGCTTACCAACAGCTTATAGCCTTTGGAGAAGAAGAATGGATGTATCTCCAAAATGCGGCATGTGCAATAAGAAGAACGAAACAGTTGATCATGCGCTTTGTGGATGCAAGAGAGCAAAGATGATTTGTGACCACGTTTTTCCACGAGTAGATGATCATATCCCCGTGGAGAATAATTTTGCCGATCGCTTAATTCACCTTGCAAGGCAGCTGAAGGAAGCAGACTTCGAAAAGGCTTGTATTGCGTTTTGGTCCGTATGGAACAATAGAAACACACGGCTTTTGGGCAGTCCGATTGTTGAATGGACACATCAGTGTGAGTGGGTTGTTAACTATTGGGCAGAAATTTCAAGGGGGAAACAGGAGGTCGTGTCGGACCATACCCATTTGAATACATGTGCACTGGAAGCTGACGGGGCTGTTACTCTCTTTGCAGACGCGGCCGTCCTCCCGACCTTAGATGGAGCTGGCCTAGGTGTCTTATTGGTTAATGAAGTCGGCAATATTTGTGGGGCAATGGAGATATATGAGAGTTCAGCTTTGTCTCCGTTCGCAGCGGAAGTTCAGGCTATAATGCATGGACTAAGATTGACTGCACGCATGGAAATACAAAGAGTTCATGTGTACTCGGACTCTTTAAATGCAATTCGGATGATCAATGGAGAGATTGGAGAGTGGCTGGAAGTTCGACACTGGATATCAGAACTTCATGATCTATGCAAAGGGTTCCTTTCAGTGTGTTTCTCTTATATCCCAAGGGATAAAAATAGGAAAGCAGATGCTCTAGCGAAGGATGCGTTAATGCACCAGCAAAATATGTTGTGGGTAGAAAATTTTCCGCTATGGCTATTATCAATGGGTAACCAATCCCACGATAAGAGTATTGTAAGTTGTGTTCGTCTCTAA

Protein sequence

MKRFLPSDSSVEVEEEEEETEAKNRGGGSAHSVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVPSSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTLCDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIFTNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKFFQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPISVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIKVFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVDDHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLGSPIVEWTHQCEWVVNYWAEISRGKQEVVSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGLGVLLVNEVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLLSMGNQSHDKSIVSCVRL
Homology
BLAST of Lag0004473 vs. NCBI nr
Match: XP_030479133.1 (uncharacterized protein LOC115696372 [Cannabis sativa])

HSP 1 Score: 446.8 bits (1148), Expect = 3.4e-121
Identity = 230/662 (34.74%), Postives = 357/662 (53.93%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  A+   L  Y  ASGQ +N +KS M FSPN P  ++ S +QI+GMPI +    YLG+P
Sbjct: 659  SCGAIKRALDIYHRASGQRLNADKSVMSFSPNTPIAVQNSFQQILGMPICECHEAYLGLP 718

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++   F  +K+++W+ +  W  K FS+GGKEVL+K+V Q+IPT+ MSCFRLPV L
Sbjct: 719  AYSERDKSQLFSNIKEKIWKLMHAWNDKIFSIGGKEVLLKAVVQSIPTYAMSCFRLPVKL 778

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C+++  MMAKFWWGS+ + KKIHWK+W F+CK K  GG+ FR    FN+ALLAKQ WRIF
Sbjct: 779  CNEIEAMMAKFWWGSSSDNKKIHWKKWRFLCKSKGDGGMGFRSFVHFNQALLAKQAWRIF 838

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
             +P  L+S+V+ G Y      ++A      S+ W+G VW R LL  G+R +VG G +I+ 
Sbjct: 839  QDPTSLLSRVLKGHYFSQNDFMTARGGGLSSLTWQGIVWGRELLVKGLRLKVGTGNNIRC 898

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              D WIP    FKP     ++ N    VAD+IT ++ WN+  +++  +  D + I  IP+
Sbjct: 899  AVDSWIPGHKGFKPYCYTGAHTNH---VADYITATREWNIECLQSDFSTPDVDNILKIPL 958

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S +   DRW+WHY  +G+Y+V SGY  A     + +S+ S+  + WW + W   LP+K+K
Sbjct: 959  SFLPVNDRWIWHYEDSGDYSVSSGYTLASSLGEEDLSSCSHTQETWWKSFWKLNLPSKVK 1018

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F WK     +P A SL+ R++  S  C +C    E++ HAL  C  AK +       +D
Sbjct: 1019 IFGWKVIQSSIPVAKSLYHRKILTSATCSLCQSAWESIGHALFSCCHAKEVWKFSGFSID 1078

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLG----SPIVEWTHQCE 511
                      D L+HL+   +++ FE      W +W++RN  + G    +P+  WT    
Sbjct: 1079 FTNADRLQDGDYLMHLSSIYEKSAFENILCLMWFIWSDRNNFIHGKKVKTPLQMWTQSVA 1138

Query: 512  WVVNYWAEISRGKQEVVSDHTHLNTCAL---EADGAVTLFADAAVLPTLDGAGLGVLLVN 571
            ++  Y   I+       S+ T   + A      +    L  DAA+  +    G+GV++ N
Sbjct: 1139 YMDQY-RSITSAVTPAASNRTSQASVASWKPPPENTFKLNVDAALDSSRSKIGIGVIVRN 1198

Query: 572  EVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIG 631
              G +  A+             E +A+  GL      ++    V +D L  +  +NG++ 
Sbjct: 1199 SAGQVKAALSTPAIGNFKSQEMEAKAMSVGLSWAKTYQLPIDFVETDCLMLVNALNGDVS 1258

Query: 632  EWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLL 687
            ++      +S++      F + C S+I R+ N+ A  LA+ AL      +W+E+ P  + 
Sbjct: 1259 QYSGFYDLVSDVKFHLSNFSNTCVSHIRRNANQAAHGLARYALQLDNECIWLEDIPSTIF 1316

BLAST of Lag0004473 vs. NCBI nr
Match: XP_030497600.1 (uncharacterized protein LOC115713257 [Cannabis sativa])

HSP 1 Score: 444.5 bits (1142), Expect = 1.7e-120
Identity = 227/661 (34.34%), Postives = 351/661 (53.10%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  ++   L  Y  ASGQ++N +KS M FSPN P  +++S +QI+GMPI      YLG+P
Sbjct: 709  SCGSIKRALDVYHRASGQLLNTDKSVMSFSPNTPEAVKLSFQQILGMPICACHESYLGLP 768

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++   F  +K+R+W+ +  W  K FS+GGKEVL+K+V QAIPT+ MSCFRL    
Sbjct: 769  AYSERDKSQLFNNIKERIWKLMHAWSDKIFSIGGKEVLLKAVVQAIPTYAMSCFRLSAKF 828

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C  +  MMA+FWWGS+ + KKIHWK W  +C  K  GGL FR    FN+A LAKQ WRIF
Sbjct: 829  CKQIETMMARFWWGSSTDNKKIHWKNWKSLCTSKRDGGLGFRSFVHFNQAFLAKQAWRIF 888

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
              P  L+S+V+ GRY H    ++A V    S+ W+G VW R LLS G+  ++G+G  +  
Sbjct: 889  QTPNSLLSRVLKGRYYHQNDFMTAKVSGLSSLTWQGIVWGRELLSKGLIIKIGDGTGVNC 948

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              D WIP    FKPLR   S  N    VAD+IT ++ W++  + N  +  D + I  IP+
Sbjct: 949  AHDSWIPGNEYFKPLRFTGSCSN---LVADYITDTREWDLELLHNDFSPADIDRILTIPL 1008

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S  +  DRW WHY  +G+YTVKSGY  A        S+SS   + WW   W   LP+K++
Sbjct: 1009 SYNSTRDRWRWHYDSSGDYTVKSGYNLACSLENKDHSSSSTSQEAWWQLFWGLNLPSKVR 1068

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F W+  +  LP A +L+ R++  S  C +C++  E++ HAL  C  AK +  H   ++D
Sbjct: 1069 IFGWRVINSALPVAQNLFHRKVITSATCSLCSRAWESIGHALFSCCHAKSVWQHTSFQLD 1128

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNT----RLLGSPIVEWTHQCE 511
                      D L+ L+  L +++ EK     W +W++RN     + L  P+   +    
Sbjct: 1129 FTKASFMKDGDYLLFLSTILTKSELEKLFCTMWFIWSDRNNYIHCKQLKHPMAISSQAEA 1188

Query: 512  WVVNYWAEISRGKQEV--VSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGLGVLLVNE 571
            ++ N+ +  S     V  V+            +  + +  DAA+  + +  G+GV++ + 
Sbjct: 1189 YLANFHSVKSATAPAVSCVAADARTVKWVPPTESNLKMNVDAALDSSRNKIGIGVIIRDS 1248

Query: 572  VGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGE 631
             G +  AM             E +A+  GL+   ++++Q   V +D L  +  + G+  +
Sbjct: 1249 TGRVIAAMSKPVVGNFKSQEMEAKAMFWGLQWAKQLQLQPHCVETDCLMLVHALQGKQSQ 1308

Query: 632  WLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLLS 687
                   + ++      F + C S++ RD N+ A  LAK AL    + +W+E  P  + S
Sbjct: 1309 LSSFHDLVEDISYHLSSFSNACISHVRRDANQAAHGLAKQALQLDNDCIWIEEIPSTIFS 1366

BLAST of Lag0004473 vs. NCBI nr
Match: XP_030502555.1 (uncharacterized protein LOC115717715 [Cannabis sativa])

HSP 1 Score: 430.3 bits (1105), Expect = 3.3e-116
Identity = 220/661 (33.28%), Postives = 343/661 (51.89%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  ++   L  Y  ASGQ++N +KS M FSPN P  +++S +QI+GMPI D    YLG+P
Sbjct: 709  SCGSIKRALDVYHRASGQLLNTDKSVMSFSPNTPDTVKLSFQQILGMPICDCHETYLGLP 768

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++   F  +K+R+W+ +  W  K FS+GGKEVL+K+V QAIPT+ MSCFRL    
Sbjct: 769  AYSERDKSQLFNNIKERIWKLMHAWNDKIFSIGGKEVLLKAVVQAIPTYAMSCFRLSTKF 828

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C  +  MMA+FWWGS+ + KKIHWK W  +C  K  G L FR    FN+A LAKQ WR+F
Sbjct: 829  CKQIESMMARFWWGSSTDNKKIHWKGWKSLCTSKGDGRLGFRSFVHFNQAFLAKQAWRVF 888

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
             NP  L+S+V+ GRY H+   LSA      S+ W+G +W R LL  G+R ++G G ++  
Sbjct: 889  QNPHSLLSRVLQGRYYHHTDFLSAKASGLSSLTWQGILWGRELLQQGLRIKIGTGTTVSC 948

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              D WIP    FKP R    + N    VA +IT ++ WN   +    +  D   I  IP+
Sbjct: 949  ANDSWIPGHDFFKPFRFTGPSNN---LVAHYITDAREWNWELLHRDFSPADVEKILTIPL 1008

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S  +  D W+WHY  +G YTVKS Y  A        ++SS   + WW   W   LP+K++
Sbjct: 1009 SYNSMSDCWIWHYECSGEYTVKSRYTLACSLENRDQTSSSGSQETWWKRFWGLTLPSKVR 1068

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F WK  +  LP A +L+ R++  S  C +C++  E++ HAL  C  AK +  +   ++D
Sbjct: 1069 IFGWKVINSALPVATNLFHRKVITSATCSLCSRVWESIGHALFSCCHAKAVWQNTGFQLD 1128

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLGSPIVEWTHQCEWVVN 511
                      D L+ L+  L  ++ E+     W +W++RN  + G  + +          
Sbjct: 1129 FQKASYMKDGDYLMFLSTILTNSELERLFCTMWFIWSDRNNFIHGKQLKQPMAISSQAEA 1188

Query: 512  YWAEISRGKQEV------VSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGLGVLLVNE 571
            Y A  +  + +       V+   +        +  + +  DAA+  +    G+GV++ + 
Sbjct: 1189 YLANFNSVQLQATPATFRVAADVNRVKWTPPPETKLKMNVDAAIDSSRSKIGMGVIIRDS 1248

Query: 572  VGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGE 631
             G++  AM             E +A+  GL+L  ++++Q  +V +D L  +  ING   +
Sbjct: 1249 SGSVVAAMSKPAVGNFKSQEMEAKAMFWGLQLAEQLQLQLHYVETDCLMLVTAINGAQSQ 1308

Query: 632  WLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLLS 687
                   + ++        + C S++ RD N+ A  LAK AL    + +W+E  P  + S
Sbjct: 1309 HSSFHDLVKDISYHLSSSSNACISHVRRDANQAAHGLAKQALQLDNDCIWIEEIPSSIFS 1366

BLAST of Lag0004473 vs. NCBI nr
Match: XP_030508852.1 (uncharacterized protein LOC115723496 [Cannabis sativa])

HSP 1 Score: 427.9 bits (1099), Expect = 1.6e-115
Identity = 241/677 (35.60%), Postives = 345/677 (50.96%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  A+H  L  Y  ASGQVIN EK  + FS N     ++  K ++GMPI     +YLG+P
Sbjct: 708  SARAIHRCLITYSRASGQVINPEKCVLSFSENTRQHEQIFFKDLLGMPIQPCHEQYLGLP 767

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            S   +N+K  F G+  ++W+ L  WK   FS GGKEVL+K+V QAIPT+ MSCFRLPVTL
Sbjct: 768  SFSGKNKKQLFGGITDKIWKLLSSWKEHLFSAGGKEVLLKAVVQAIPTYAMSCFRLPVTL 827

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C  +  MMA+FWWGST   K IHWK W+F+CK K  GGL FR+   FN+ALLAKQ WRI 
Sbjct: 828  CHQIESMMARFWWGSTATGKTIHWKNWNFLCKAKVQGGLGFRNFIHFNQALLAKQAWRIL 887

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
              P  L+S ++  RY  N + L A + SN S+ WR  VW + LL  G+R +VG+G  I  
Sbjct: 888  EFPNSLLSNLLRHRYFSNGNYLIAGLGSNPSLTWRSLVWGKELLLKGLRWRVGSGERINC 947

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              D W+P  T+FKP      + N    VAD IT  + W++  +E    + D N + +IP+
Sbjct: 948  KTDSWLPGHTTFKPYFFKGPDPN--LLVADLITEHRTWDMISLETNFNQADINRVLSIPL 1007

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S    +D  +W+ S  G Y VKSGY  A+       ST SN  +HWW   W  KLP K++
Sbjct: 1008 SPYPHDDVLIWNQSFTGVYNVKSGYHFAVSLAEQDDSTCSNSIEHWWSNFWKLKLPPKVR 1067

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F+WK FH  LP A  L+RR +  SP C +CN   ETV HAL  C RAK + +     +D
Sbjct: 1068 IFVWKVFHTSLPVAAELYRRHIAFSPYCTICNSCEETVHHALFSCPRAKAVWELSNFSID 1127

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLGSPIVEWTHQCEWVVN 511
                  ++ AD L+ L+  L  ++ E   +  WS+W+ RN    G+ +        +  +
Sbjct: 1128 FQTIERSSTADTLLLLSTSLSSSELELFLVLCWSIWHERNAIYHGNSVRTPAAVAAYAPS 1187

Query: 512  YWAEISRGKQEVV---------------SDHTHLNTCALEADGAVTLFADAAVLPTLDGA 571
            Y  E  + + +                 S+  H         G + L  DAA+    +  
Sbjct: 1188 YLTEFQQARAKNAKPVTASGAATPSRPSSEFIHAPKWTTPPRGRLKLNTDAAIDKERNTI 1247

Query: 572  GLGVLLVNEVGNICGAMEIYESSALSPF-----AAEVQAIMHGLRLTARM--EIQRVHVY 631
            G+G +L N  G I  A+         PF     A E++A+   L L   +   +    + 
Sbjct: 1248 GIGAVLRNSDGIIVAALS-------KPFRGNFKAEEMEALGLALSLNWLLSHNLSVDFIE 1307

Query: 632  SDSLNAIRMINGEIGEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMH 687
            +DSL  ++ +             ++ ++ L   F      ++ R  N  A  LAK AL  
Sbjct: 1308 TDSLLVVQGLKTSHSFLSAFHALLNNINYLVSFFPRAQIDHVSRSANTYAHTLAKFALTV 1367

BLAST of Lag0004473 vs. NCBI nr
Match: XP_030509050.1 (uncharacterized protein LOC115723712 [Cannabis sativa])

HSP 1 Score: 423.7 bits (1088), Expect = 3.1e-114
Identity = 237/702 (33.76%), Postives = 355/702 (50.57%), Query Frame = 0

Query: 29   SAHSVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYL 88
            S  S  A+H  L  Y  ASGQ++N EKS + FSPN     +   + ++ MPI     +YL
Sbjct: 705  SRSSARAIHLCLDLYSRASGQMLNPEKSVLSFSPNTRTTEQHFFQHLLNMPIQPCHEQYL 764

Query: 89   GVPSSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLP 148
            G+PS   R++   F G+  ++W+ +  W+ + FS+GGKEVL+K+V QAIPT+ MSCF+LP
Sbjct: 765  GLPSFSGRDKSQLFSGITDKIWKLMNAWREQLFSIGGKEVLLKAVVQAIPTYAMSCFQLP 824

Query: 149  VTLCDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVW 208
            V LC+ + ++M++FWWG +     IHWK W F+CK K  GG+ FR+   FN+ALLAKQ W
Sbjct: 825  VKLCNQIEQLMSRFWWGKSANGNAIHWKNWKFLCKSKVHGGMGFRNFVHFNQALLAKQAW 884

Query: 209  RIFTNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGAS 268
            RI  +P  L+++V+  RY      L A   +  S+ W+  VW + LL  G+R ++G+GA 
Sbjct: 885  RILEDPSSLIARVLKSRYFATGVFLEATAGAQPSLTWKSIVWGKELLLKGLRWRIGSGAD 944

Query: 269  IKFFQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAA 328
            +     PWIP  ++FKPL     + + D  VADFIT S+ W+V K++   T  D + I +
Sbjct: 945  VNCVSQPWIPGPSNFKPLL--FLSPDRDVKVADFITHSRQWDVPKLQQFFTPPDVDRILS 1004

Query: 329  IPISVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPT 388
            IP+S+   ED  +WHYS  G YTVKSGYK A     D+   SS+ T+ WW   W  KLP+
Sbjct: 1005 IPLSLFPCEDVLLWHYSSAGFYTVKSGYKLA-SDVPDSQPNSSHTTETWWQYFWGLKLPS 1064

Query: 389  KIKVFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFP 448
            KI++F W+A+HE LPTA  L  R +  SP+C +C    ET++HA   C RAK +  +   
Sbjct: 1065 KIRIFAWRAYHEALPTAAILQYRHISSSPQCPLCQVHLETINHAFFWCNRAKQVWKNWDE 1124

Query: 449  RVDDHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLGSPIVEWTHQCEW 508
             ++  + +  +F+D L++++ QL+    E      W +W  RN               ++
Sbjct: 1125 SINWSLSMTCSFSDFLVYVSSQLQAEKVELFLTTLWCIWTARNAETHSKSPKSALQIFQF 1184

Query: 509  VVNYWAEI--------SRGKQEVVSDHTHLNTCALEA----------------------- 568
              +Y AE         S G +  VS  T LN+ A  +                       
Sbjct: 1185 ASSYLAEFRKAQAKTNSAGTRFQVSPATSLNSAAESSAQLSHLVAASDSAHQAFTYDPTG 1244

Query: 569  -------------DGAVTLFADAAVLPTLDGAGLGVLLVNEVGNICGAMEIYESSALSPF 628
                          G + +  DAAV       GL  +L N  G+I  AM       +   
Sbjct: 1245 VLPPTKPKWLAPPSGRLKMNTDAAVNVATGVFGLAAILRNSTGDILAAMVKPMKGCVKAE 1304

Query: 629  AAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISELHDLCKGFL 687
              E  AI  GL+L   + +    + SDSL  +  +             ++++  L   F 
Sbjct: 1305 EMEALAIAWGLKLMLHLRLNVDFIESDSLMVVNGLKSHFKGLSAFHVILNDITFLLSNFP 1364

BLAST of Lag0004473 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.2e-47
Identity = 166/605 (27.44%), Postives = 268/605 (44.30%), Query Frame = 0

Query: 90  VPSSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPV 149
           +P    R  KD F  + +RV   + GW+ K  S  G+  L K+V  ++P   MS   LP 
Sbjct: 1   MPVLQKRINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQ 60

Query: 150 TLCDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWR 209
           ++ + L ++   F WGST EKKK H  +W  +C PK+ GGL  R  K  N+AL++K  WR
Sbjct: 61  SILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWR 120

Query: 210 IFTNPGLLVSKVIHGRYAHNMSVLSAP---VKSNCSVFWRGF-VWARGLLSHGMRKQVGN 269
           +      L + V+  +Y H   +  +     K + S  WR   +  R ++SHG+    G+
Sbjct: 121 LLQEKNSLWTLVLQKKY-HVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGD 180

Query: 270 GASIKFFQDPWIPKETSFKPLRIDMSN----INDDPWVA-DFITPSKGWNVAKIENVVTK 329
           G  I+F+ D W+    S KPL +++ N     + D  VA D   P +GW+ AKI+   T 
Sbjct: 181 GQQIRFWTDRWV----SGKPL-LELDNGERPTDCDTVVAKDLWIPGRGWDFAKIDPYTTN 240

Query: 330 EDANVIAAIPISVV-NEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWG 389
                + A+ + +V    DR  W +S++G ++V+S Y+   M TVD V   +     ++ 
Sbjct: 241 NTRLELRAVVLDLVTGARDRLSWKFSQDGQFSVRSAYE---MLTVDEVPRPN--MASFFN 300

Query: 390 ALWNSKLPTKIKVFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRA 449
            LW  ++P ++K F+W   ++ + T     RR +  S  C +C    E++ H L  C   
Sbjct: 301 CLWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQ 360

Query: 450 KMICDHVFP-RVDDHIPVENNFADRLIHLARQLKEADFEKA---CIAFWSVWNNRNTRLL 509
             I   V P R       ++ F     +L  +    D   +    +  W  W  R   + 
Sbjct: 361 LGIWVRVVPQRRQQGFFSKSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCGNIF 420

Query: 510 GSPIVEWTHQCEWVVNYWAEISRGKQ-EVVSDHTH---------LNTCALEADGAVTLFA 569
           G    +   + ++V  +  E+ R     V+   T          ++ C     G V +  
Sbjct: 421 GEN-TKCRDRVKFVKEWAVEVYRAHSGNVLVGITQPRVERMIGWVSPCV----GWVKVNT 480

Query: 570 DAAVLPTLDGAGLGVLLVNEVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQR 629
           D A       A  G +L +  G  CG   +      +P  AE+  + +GL      ++ R
Sbjct: 481 DGASRGNPGLASAGGVLRDCTGAWCGGFSLNIGRCSAP-QAELWGVYYGLYFAWEKKVPR 540

Query: 630 VHVYSDSLNAIRMINGEIGEWLEVRHWISELHDLCKGFLS----VCFSYIPRDKNRKADA 667
           V +  DS     +I G +   +   H +S L  LC GFL     V   ++ R+ NR AD 
Sbjct: 541 VELEVDS----EVIVGFLKTGISDSHPLSFLVRLCHGFLQKDWLVRIVHVYREANRLADG 584

BLAST of Lag0004473 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 3.4e-31
Identity = 68/152 (44.74%), Postives = 89/152 (58.55%), Query Frame = 0

Query: 136 AIPTFIMSCFRLPVTLCDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKE-LGGLNFRD 195
           A+P + MSCFRL   LC  L   M +FWW S E K+KI W  W  +CK KE  GGL FRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 196 LKVFNKALLAKQVWRIFTNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGL 255
           L  FN+ALLAKQ +RI   P  L+S+++  RY  + S++   V +  S  WR  +  R L
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 256 LSHGMRKQVGNGASIKFFQDPWIPKETSFKPL 287
           LS G+ + +G+G   K + D WI  ET   PL
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPLPPL 153

BLAST of Lag0004473 vs. ExPASy TrEMBL
Match: A0A803QGT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.6e-121
Identity = 230/662 (34.74%), Postives = 357/662 (53.93%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  A+   L  Y  ASGQ +N +KS M FSPN P  ++ S +QI+GMPI +    YLG+P
Sbjct: 660  SCGAIKRALDIYHRASGQRLNADKSVMSFSPNTPIAVQNSFQQILGMPICECHEAYLGLP 719

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++   F  +K+++W+ +  W  K FS+GGKEVL+K+V Q+IPT+ MSCFRLPV L
Sbjct: 720  AYSERDKSQLFSNIKEKIWKLMHAWNDKIFSIGGKEVLLKAVVQSIPTYAMSCFRLPVKL 779

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C+++  MMAKFWWGS+ + KKIHWK+W F+CK K  GG+ FR    FN+ALLAKQ WRIF
Sbjct: 780  CNEIEAMMAKFWWGSSSDNKKIHWKKWRFLCKSKGDGGMGFRSFVHFNQALLAKQAWRIF 839

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
             +P  L+S+V+ G Y      ++A      S+ W+G VW R LL  G+R +VG G +I+ 
Sbjct: 840  QDPTSLLSRVLKGHYFSQNDFMTARGGGLSSLTWQGIVWGRELLVKGLRLKVGTGNNIRC 899

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              D WIP    FKP     ++ N    VAD+IT ++ WN+  +++  +  D + I  IP+
Sbjct: 900  AVDSWIPGHKGFKPYCYTGAHTNH---VADYITATREWNIECLQSDFSTPDVDNILKIPL 959

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S +   DRW+WHY  +G+Y+V SGY  A     + +S+ S+  + WW + W   LP+K+K
Sbjct: 960  SFLPVNDRWIWHYEDSGDYSVSSGYTLASSLGEEDLSSCSHTQETWWKSFWKLNLPSKVK 1019

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F WK     +P A SL+ R++  S  C +C    E++ HAL  C  AK +       +D
Sbjct: 1020 IFGWKVIQSSIPVAKSLYHRKILTSATCSLCQSAWESIGHALFSCCHAKEVWKFSGFSID 1079

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLG----SPIVEWTHQCE 511
                      D L+HL+   +++ FE      W +W++RN  + G    +P+  WT    
Sbjct: 1080 FTNADRLQDGDYLMHLSSIYEKSAFENILCLMWFIWSDRNNFIHGKKVKTPLQMWTQSVA 1139

Query: 512  WVVNYWAEISRGKQEVVSDHTHLNTCAL---EADGAVTLFADAAVLPTLDGAGLGVLLVN 571
            ++  Y   I+       S+ T   + A      +    L  DAA+  +    G+GV++ N
Sbjct: 1140 YMDQY-RSITSAVTPAASNRTSQASVASWKPPPENTFKLNVDAALDSSRSKIGIGVIVRN 1199

Query: 572  EVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIG 631
              G +  A+             E +A+  GL      ++    V +D L  +  +NG++ 
Sbjct: 1200 SAGQVKAALSTPAIGNFKSQEMEAKAMSVGLSWAKTYQLPIDFVETDCLMLVNALNGDVS 1259

Query: 632  EWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLL 687
            ++      +S++      F + C S+I R+ N+ A  LA+ AL      +W+E+ P  + 
Sbjct: 1260 QYSGFYDLVSDVKFHLSNFSNTCVSHIRRNANQAAHGLARYALQLDNECIWLEDIPSTIF 1317

BLAST of Lag0004473 vs. ExPASy TrEMBL
Match: A0A803PIB6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 8.2e-121
Identity = 227/661 (34.34%), Postives = 351/661 (53.10%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  ++   L  Y  ASGQ++N +KS M FSPN P  +++S +QI+GMPI      YLG+P
Sbjct: 1169 SCGSIKRALDVYHRASGQLLNTDKSVMSFSPNTPEAVKLSFQQILGMPICACHESYLGLP 1228

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++   F  +K+R+W+ +  W  K FS+GGKEVL+K+V QAIPT+ MSCFRL    
Sbjct: 1229 AYSERDKSQLFNNIKERIWKLMHAWSDKIFSIGGKEVLLKAVVQAIPTYAMSCFRLSAKF 1288

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C  +  MMA+FWWGS+ + KKIHWK W  +C  K  GGL FR    FN+A LAKQ WRIF
Sbjct: 1289 CKQIETMMARFWWGSSTDNKKIHWKNWKSLCTSKRDGGLGFRSFVHFNQAFLAKQAWRIF 1348

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
              P  L+S+V+ GRY H    ++A V    S+ W+G VW R LLS G+  ++G+G  +  
Sbjct: 1349 QTPNSLLSRVLKGRYYHQNDFMTAKVSGLSSLTWQGIVWGRELLSKGLIIKIGDGTGVNC 1408

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              D WIP    FKPLR   S  N    VAD+IT ++ W++  + N  +  D + I  IP+
Sbjct: 1409 AHDSWIPGNEYFKPLRFTGSCSN---LVADYITDTREWDLELLHNDFSPADIDRILTIPL 1468

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S  +  DRW WHY  +G+YTVKSGY  A        S+SS   + WW   W   LP+K++
Sbjct: 1469 SYNSTRDRWRWHYDSSGDYTVKSGYNLACSLENKDHSSSSTSQEAWWQLFWGLNLPSKVR 1528

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F W+  +  LP A +L+ R++  S  C +C++  E++ HAL  C  AK +  H   ++D
Sbjct: 1529 IFGWRVINSALPVAQNLFHRKVITSATCSLCSRAWESIGHALFSCCHAKSVWQHTSFQLD 1588

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNT----RLLGSPIVEWTHQCE 511
                      D L+ L+  L +++ EK     W +W++RN     + L  P+   +    
Sbjct: 1589 FTKASFMKDGDYLLFLSTILTKSELEKLFCTMWFIWSDRNNYIHCKQLKHPMAISSQAEA 1648

Query: 512  WVVNYWAEISRGKQEV--VSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGLGVLLVNE 571
            ++ N+ +  S     V  V+            +  + +  DAA+  + +  G+GV++ + 
Sbjct: 1649 YLANFHSVKSATAPAVSCVAADARTVKWVPPTESNLKMNVDAALDSSRNKIGIGVIIRDS 1708

Query: 572  VGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGE 631
             G +  AM             E +A+  GL+   ++++Q   V +D L  +  + G+  +
Sbjct: 1709 TGRVIAAMSKPVVGNFKSQEMEAKAMFWGLQWAKQLQLQPHCVETDCLMLVHALQGKQSQ 1768

Query: 632  WLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLLS 687
                   + ++      F + C S++ RD N+ A  LAK AL    + +W+E  P  + S
Sbjct: 1769 LSSFHDLVEDISYHLSSFSNACISHVRRDANQAAHGLAKQALQLDNDCIWIEEIPSTIFS 1826

BLAST of Lag0004473 vs. ExPASy TrEMBL
Match: A0A803PKJ2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 2.9e-118
Identity = 222/666 (33.33%), Postives = 346/666 (51.95%), Query Frame = 0

Query: 32  SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
           S  ++   L  Y  ASGQ++N +KS M FSPN P  +++S +QI+GMPI D    YLG+P
Sbjct: 206 SCGSIKRALDVYHRASGQLLNTDKSVMSFSPNTPDTVKLSFQQILGMPICDCHETYLGLP 265

Query: 92  SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
           +   R++   F  +K+R+W+ +  W  K FS+GG EVL+K+V QAIPT+ MSCFRL    
Sbjct: 266 AYSERDKSQLFNNIKERIWKLMHAWNDKKFSIGGNEVLLKAVVQAIPTYAMSCFRLSTKF 325

Query: 152 CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
           C  +  MMA+FWWGS+ + KKIHWK W  +C  K  GGL FR    FN+A LAKQ WR+F
Sbjct: 326 CKQIESMMARFWWGSSTDNKKIHWKSWKSLCTSKGDGGLGFRSFVHFNQAFLAKQAWRVF 385

Query: 212 TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
            NP  L+S+V+ GRY H+   LSA      S+ W+G +W R LL  G+R ++G G ++  
Sbjct: 386 QNPHSLLSRVLKGRYYHHNDFLSAKASGLSSLTWQGIIWGRELLQQGLRIKIGTGTAVSC 445

Query: 272 FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
             D WIP    FKP +    + N   WVA +IT ++ WN   +    +  D   I  IP+
Sbjct: 446 ANDSWIPGHDFFKPFQFTGPSNN---WVAQYITDAREWNWELLHRDFSPVDVEKILTIPL 505

Query: 332 SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
           S  +  D W+WHY  +G YTVKSGY  A        ++SS   + WW   W   LP+K++
Sbjct: 506 SYSSMTDCWIWHYDCSGEYTVKSGYTLACSLENRDQTSSSGSQETWWKRFWGLTLPSKVR 565

Query: 392 VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
           +F WK  +  LP A +L+ +++  S  C +C++  E++ HAL  C  AK +  +    +D
Sbjct: 566 IFGWKVINSALPVATNLFHQKVITSATCSLCSRAWESIGHALFSCCHAKAVWQNTSFHLD 625

Query: 452 DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLGSPIVEWTHQCEWVVN 511
                     D L+ L+  L +++ E+     W +W++RN  + G  + +        ++
Sbjct: 626 FQKASYMKDGDYLMFLSTILTKSELERLFCTMWFIWSDRNNFIHGKQLKQ-----PMAIS 685

Query: 512 YWAEISRGKQEVVSDHTHLNTCALEAD-----------GAVTLFADAAVLPTLDGAGLGV 571
             AE+     + V  HT      + AD             + +  DAA+  +    G+GV
Sbjct: 686 SQAEVYLANFKSVQLHTTPVAFCVAADVNQMKWIPPPETKLKMNVDAAIDSSRSKIGIGV 745

Query: 572 LLVNEVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMIN 631
           ++ +  G++  AM             E +A+  GL+    +++Q  +V +D +  +  IN
Sbjct: 746 IIRDSSGSVVVAMSKPTVGNFKSQEMEAKAMFWGLQWAQHLQLQLHYVETDGMMLVTAIN 805

Query: 632 GEIGEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFP 687
           G   +       + ++        + C S++ RD N+ A  LAK AL    + +W+E  P
Sbjct: 806 GTQSQHSSFHDLVKDISYHLSSSSNACISHVRRDANQAAHDLAKQALQLDNDCIWIEEIP 863

BLAST of Lag0004473 vs. ExPASy TrEMBL
Match: A0A803NM27 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 6.5e-118
Identity = 234/663 (35.29%), Postives = 343/663 (51.73%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  A+  +L  Y  ASGQ +N +KS M FSPN     + S + I+GMPI +    YLG+P
Sbjct: 1117 SCGAIKRVLDTYHKASGQQLNTDKSVMSFSPNTSEASKQSFQNILGMPICECHESYLGLP 1176

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++K  F  +K+R+W+ L  W  K FS+GGKEVL+K+V Q+IPT+ MSCF+LPV  
Sbjct: 1177 AYSERDKKQLFNQIKERIWKLLNAWTDKIFSVGGKEVLLKAVIQSIPTYAMSCFKLPVKF 1236

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C ++  +M+ FWWGST +KKKIHWK+W F+CK K  GGL FR+   FN+ALLAKQ WR+F
Sbjct: 1237 CHEIESLMSNFWWGSTSDKKKIHWKQWKFLCKSKLEGGLGFRNFIHFNQALLAKQAWRLF 1296

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
             NP  L+ +V+ GRY      LSA      S+ W+GF W R LL  G+R QVGNG  I  
Sbjct: 1297 QNPESLLFRVLKGRYFSRSDFLSAATCGVSSLTWQGFCWGRELLKKGLRLQVGNGFDIAC 1356

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              DPWIP  + F P+    +  N    VAD+ITP K WNV+K+    +  D   I ++P+
Sbjct: 1357 ATDPWIPGNSIFTPIYFTGAPTNT---VADYITPEKEWNVSKLNADFSSADVERILSLPL 1416

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S     D WVWH +  G Y VKSGY  A +   +   + S+    WW + W  KLP K+K
Sbjct: 1417 SHHAHSDYWVWH-ATGGQYEVKSGYHVACLLADENPVSVSSPNVSWWKSFWQLKLPPKVK 1476

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F WKA H  LP A  L++R+   S  C +C    E+V HA+  CK A+ +        +
Sbjct: 1477 LFAWKAIHNALPVASELYKRKSLTSASCSLCLNAWESVGHAMFACKHARHVWKIAGFSFN 1536

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLG----SPIVEWTHQCE 511
            +   V     D L  ++    +++ E      WS+W++RN  L G     P V       
Sbjct: 1537 NKAAVSMKIEDFLFQISECYTKSELEMIFCTMWSIWSDRNNVLHGKIPQQPSVISAKAAS 1596

Query: 512  WVVNYWA----EISRGKQEVVSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGLGVLLV 571
            ++ ++ +     +  G     +   H           + L  DAA   T    G G ++ 
Sbjct: 1597 FLSSFQSAQQLSLHAGLAPADTPTAH-RAWTPPPPNLLKLNVDAAFDDTRKRIGFGAIIR 1656

Query: 572  NEVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEI 631
            +  GN+  AM         P   E + + + L+   ++  +   V +DSL  +  +    
Sbjct: 1657 DSTGNVKAAMSHPIDGCCRPQDMEAKGLFYSLKWARQLNFKVDLVETDSLILVNALRKST 1716

Query: 632  GEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWL 687
             +    +  I ++        +VC  ++ RD N+ A  LAK AL+      W+E+FP  +
Sbjct: 1717 SKSSSFQDLIFDVQTQLSYLPTVCVHHVYRDGNQAAHGLAKHALVLDNVCTWLEDFPSAI 1774

BLAST of Lag0004473 vs. ExPASy TrEMBL
Match: A0A803Q8J4 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 5.5e-117
Identity = 233/662 (35.20%), Postives = 345/662 (52.11%), Query Frame = 0

Query: 32   SVTALHSLLKKYEIASGQVINVEKSAMFFSPNVPAELRVSLKQIMGMPIVDNLGRYLGVP 91
            S  A+  +L  Y  ASGQ +N +KS M FSPN     ++S + I+GMPI D    YLG+P
Sbjct: 1141 SCGAIKRVLDTYHKASGQQLNTDKSVMSFSPNTTEVAKLSFRNILGMPICDCHESYLGLP 1200

Query: 92   SSFSRNRKDDFKGVKQRVWQTLQGWKGKFFSMGGKEVLIKSVAQAIPTFIMSCFRLPVTL 151
            +   R++K+ F  +K+R+W+ L  W  K FS+GGKEVL+K+V Q+IPT+ MSCF+LPV  
Sbjct: 1201 AYSERDKKNMFNHIKERIWKLLHAWTDKIFSVGGKEVLLKAVIQSIPTYAMSCFKLPVKF 1260

Query: 152  CDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDLKVFNKALLAKQVWRIF 211
            C ++  MM+ +WWG+T +KKKIHWK+W F+C  K  GGL FR+   FN+ALLAKQ WR+F
Sbjct: 1261 CREIESMMSNYWWGTTTDKKKIHWKQWKFLCHSKMEGGLGFRNFIHFNQALLAKQAWRLF 1320

Query: 212  TNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKF 271
             N   L+ +V+ GRY      LSA      S+ W+G  W R LL  G+RK+VGNG SI  
Sbjct: 1321 QNHNSLLFRVLKGRYFPRNDFLSAATCGASSLTWQGICWGRELLKKGIRKKVGNGFSISC 1380

Query: 272  FQDPWIPKETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPI 331
              DPWIP    F P+     + N +  VAD+ITP + WN +K+    +  D   I ++P+
Sbjct: 1381 ANDPWIPGNLIFTPI---FYSGNPNSVVADYITPDREWNFSKLSADFSSVDVGRILSLPL 1440

Query: 332  SVVNEEDRWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGALWNSKLPTKIK 391
            S     D W+WH    G Y VKSGY  A  ++ DT  + SN    WW + W  KLP K+K
Sbjct: 1441 SNNAGPDYWIWH-GTGGEYEVKSGYFFACSSSADTNESVSNPNTAWWKSFWQLKLPPKVK 1500

Query: 392  VFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVD 451
            +F WKA H  LP A  L++++   S  C +C    E+V HAL  CK A+ +        +
Sbjct: 1501 IFSWKAIHNALPVAAELFKKKSLTSASCSLCLNAWESVGHALFSCKHARHVWKVAGFTFN 1560

Query: 452  DHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLLG----SPIVEWTHQCE 511
            +   V  N  D L  ++    +++ E      WS+W++RN  + G     P V       
Sbjct: 1561 NKAAVSMNIEDFLFQISENYTKSELETIFCTMWSIWSDRNNVIHGKSPQQPTVISAKAQN 1620

Query: 512  WVVNYWAEISRGKQEVVSDHTHLNTCALEADGAVT---LFADAAVLPTLDGAGLGVLLVN 571
            ++ NY +         +S     +     +    T   L  DAA     +  G G ++ +
Sbjct: 1621 FLNNYKSTQQLSLPAGLSPPATPSISKAWSPPPPTCLKLNVDAAFDEACNKIGFGAIIRD 1680

Query: 572  EVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIG 631
              GN+  AM    +    P   E + + + L+   ++  +   V +DSL     +     
Sbjct: 1681 SNGNVKAAMSHPINGCCLPQEMEAKGLFYSLKWARQLNFKVDMVETDSLILANALRKTAS 1740

Query: 632  EWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWVENFPLWLL 687
                 +  I ++        +VC +++ RD N+ A  LAK AL+      W+E+FP  +L
Sbjct: 1741 NRSSFQDLIFDVQTELSYLPTVCVNHVYRDGNQAAHGLAKQALVLDNVCTWLEDFPSAIL 1798

BLAST of Lag0004473 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 218.8 bits (556), Expect = 1.4e-56
Identity = 161/568 (28.35%), Postives = 260/568 (45.77%), Query Frame = 0

Query: 136 AIPTFIMSCFRLPVTLCDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKELGGLNFRDL 195
           A+PT+ M+CF LP T+C  +  ++A FWW + +E K +HWK WD +   K  GG+ F+D+
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 196 KVFNKALLAKQVWRIFTNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGLL 255
           + FN ALL KQ+WR+ + P  L++KV   RY H    L+AP+ S  S  W+    ++ +L
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 256 SHGMRKQVGNGASIKFFQDPWIPKETSFKPLRI------DMSNINDDPWVADFITPS-KG 315
             G R  VGNG  I  ++  W+  + +   LR+      + ++++    V+D I  S + 
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGRE 181

Query: 316 WNVAKIENVVTKEDANVIAAIPISVVNEEDRWVWHYSKNGNYTVKSGY--------KQAI 375
           W    IE +  + +  +I  +        D + W Y+ +G+YTVKSGY        K++ 
Sbjct: 182 WRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSS 241

Query: 376 MATVDTVSTSSNYTKHWWGALWNSKLPTKIKVFIWKAFHECLPTAYSLWRRRMDVSPKCG 435
              V   S +  Y K     +W S+   KI+ F+WK     LP A +L  R +     C 
Sbjct: 242 PQEVSEPSLNPIYQK-----IWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACI 301

Query: 436 MCNKKNETVDHALCGCKRAKMICDHVFPRVDDHIPVENNFADRL---IHLARQLKEAD-- 495
            C    ETV+H L  C  A++     +      IP+   +AD +   ++    L   +  
Sbjct: 302 RCPSCKETVNHLLFKCTFARL----TWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQ 361

Query: 496 FEKAC----IAFWSVWNNRNTRLLGSPIVEWTHQ----------CEWVVNYWAEISRGKQ 555
           +EKA        W +W NRN  +      E+  Q           EW +   AE    K 
Sbjct: 362 WEKASQLVPWLLWRLWKNRNELVFRGR--EFNAQEVLRRAEDDLEEWRIRTEAESCGTKP 421

Query: 556 EVVSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGLGVLLVNEVGNI--CGAMEIYESS 615
           +V  + +            V    DA      +  G+G +L NE G +   GA  +    
Sbjct: 422 QV--NRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARAL--PK 481

Query: 616 ALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAIRMINGEIGEWLEVRHWISELHDL 668
             S   AE++A+   +   +R +   V   SDS   I ++N +   W  ++  I +L  L
Sbjct: 482 LKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQRL 541

BLAST of Lag0004473 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 138.3 bits (347), Expect = 2.4e-32
Identity = 68/152 (44.74%), Postives = 89/152 (58.55%), Query Frame = 0

Query: 136 AIPTFIMSCFRLPVTLCDDLHKMMAKFWWGSTEEKKKIHWKRWDFMCKPKE-LGGLNFRD 195
           A+P + MSCFRL   LC  L   M +FWW S E K+KI W  W  +CK KE  GGL FRD
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 196 LKVFNKALLAKQVWRIFTNPGLLVSKVIHGRYAHNMSVLSAPVKSNCSVFWRGFVWARGL 255
           L  FN+ALLAKQ +RI   P  L+S+++  RY  + S++   V +  S  WR  +  R L
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 256 LSHGMRKQVGNGASIKFFQDPWIPKETSFKPL 287
           LS G+ + +G+G   K + D WI  ET   PL
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPLPPL 153

BLAST of Lag0004473 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 2.8e-28
Identity = 116/487 (23.82%), Postives = 209/487 (42.92%), Query Frame = 0

Query: 225 RYAHNMSVLSAPVKSNCSVFWRGFVWARGLLSHGMRKQVGNGASIKFFQD------PWIP 284
           RY  ++S+L A V+   S  W   +    LL  G R  +G+G +I+   D      P  P
Sbjct: 4   RYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHPPRP 63

Query: 285 KETSFKPLRIDMSNINDDPWVADFITPSKGWNVAKIENVVTKEDANVIAAIPISVVNEED 344
             T      + ++N+ +      F      W+ +KI   V + D   I  I ++   + D
Sbjct: 64  LNTEETYKEMTINNLFERKGSYYF------WDDSKISQFVDQSDHGFIHRIYLAKSKKPD 123

Query: 345 RWVWHYSKNGNYTVKSGYKQAIMATVDTVSTSSNYTKHWWGA------LWNSKLPTKIKV 404
           + +W+Y+  G YTV+SGY    + T D  ST+        G+      +WN  +  K+K 
Sbjct: 124 KIIWNYNTTGEYTVRSGY---WLLTHDP-STNIPAINPPHGSIDLKTRIWNLPIMPKLKH 183

Query: 405 FIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFPRVDD 464
           F+W+A  + L T   L  R M + P C  C+++NE+++HAL  C  A M        +  
Sbjct: 184 FLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIR 243

Query: 465 HIPVENNFADRLIHLARQLKE---ADFEKACIAF--WSVWNNRN----TRLLGSPIVEWT 524
           +  + N+F + + ++   +++   +DF K    +  W +W  RN     +   SP  +  
Sbjct: 244 NQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESP-SKTV 303

Query: 525 HQCEWVVNYWAEISRGKQEVVSDHTHLNTCALEADGAVTLFA----DAAV-LPTLDGAGL 584
              +   + W   ++  ++  S    +    +E       +     DA   +  L+  G 
Sbjct: 304 LSAKAETHDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATG- 363

Query: 585 GVLLVNEVGN--ICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSLNAI 644
           G ++ N  G     G+M++  +S  +P  AE +A++  L+ T      +V +  D    I
Sbjct: 364 GWIIRNHYGTPISWGSMKLAHTS--NPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLI 423

Query: 645 RMINGEIGEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNMLWV 684
            +ING I     + + + ++      F S+ F +I R  N+ A  LAK    +       
Sbjct: 424 NLING-ISFHSSLANHLEDISFWANKFASIQFGFIRRKGNKLAHVLAKYGCTYSTFYSGS 475

BLAST of Lag0004473 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 1.5e-13
Identity = 76/325 (23.38%), Postives = 121/325 (37.23%), Query Frame = 0

Query: 381 LWNSKLPTKIKVFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAK 440
           +W  K   KIK F+WK     L T  +L RR +   P+C  C +++ET  H    C  A+
Sbjct: 18  IWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYAQ 77

Query: 441 MI-----CDHVFPRVDDHIPVENNFADRLIHLARQLKEADFEKACIAFWSVWNNRNTRLL 500
            +       H   R    I +E      L       +   F  A    W +W +RN  + 
Sbjct: 78  QVWRASGIPHQELRTTG-ITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSRNQLVF 137

Query: 501 GSPIVEWTHQCEWV---VNYWAE----ISRGKQEVVSDHTHLNTCA-----LEADGAVTL 560
               + W +  +     V  W +    +    Q+V S      T A           +  
Sbjct: 138 QQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHSSRHQQPTMARTKWQRPPSTWIKY 197

Query: 561 FADAAVLPTLDGAGLGVLLVNEVGNICGAMEIYESSALSPFAAEVQAIMHGLRLTARMEI 620
             D A       A  G L+ +E G   G+ +   S+      +E QA++  ++       
Sbjct: 198 NYDGAFNHQTRNAKAGWLMRDENGVYMGSGQAIGSTTSDSLESEFQALIIAMQHAWSQGY 257

Query: 621 QRVHVYSDSLNAIRMINGE---IGEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKAD 680
           ++V    DS     ++N E    G +    +WI E     K F    F ++PR  N+ AD
Sbjct: 258 RKVIFEGDSKQVEELMNNEKLNFGRF----NWIREGRFWQKRFEEAVFKWVPRTNNQPAD 317

Query: 681 ALAKDALMHQQNMLWVENFPLWLLS 686
            LAK  L   Q+  +    P ++ S
Sbjct: 318 ILAKHHLQPNQSFKFHYYVPAFITS 337

BLAST of Lag0004473 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 6.8e-11
Identity = 79/300 (26.33%), Postives = 120/300 (40.00%), Query Frame = 0

Query: 389 KIKVFIWKAFHECLPTAYSLWRRRMDVSPKCGMCNKKNETVDHALCGCKRAKMICDHVFP 448
           KIK+F+WKA    LP    L RR +  +  C  C    ET  H L  C  A  +  ++ P
Sbjct: 3   KIKLFLWKAAAGALPVGAQLVRRHISSTWDCARCGAP-ETSTHMLFHCDFAAQVW-NLAP 62

Query: 449 RVDDHIPVENNFADRLIHLARQ--LKEADFEKACI---AFWSVWNNRNTRLLGS---PIV 508
               HIP+     + L  L +   L       A +     W +W  RN  +  +    ++
Sbjct: 63  LQLGHIPIGTPILEALNLLKKTIVLPPVGIHTATLFPWICWHIWKARNQLIFQNSHFSVI 122

Query: 509 EWTHQCEWVVNYW--AEISRGKQEVVSDHTHLNTCALEADGAVTLFADAAVLPTLDGAGL 568
           E   +       W  A+++  K  V +   + +T  L  D     + DAA       AG 
Sbjct: 123 ETVTKAVQDALAWQSAQLALPKVRVATPRPN-STTTLTHD--FLCYVDAAWQQQSSLAGS 182

Query: 569 GVLLVNEVGNICGAMEIYESSA-----LSPFAAEVQAIMHGLRLTARMEIQRVHVYSDSL 628
           G +            EI   SA      SP AAE  AI   +    ++E   + V SDS 
Sbjct: 183 GWVF---QATSHSEKEITTFSAGCRRFPSPLAAEAWAIKSAMLHALQLERSDLLVLSDSK 242

Query: 629 NAIRMINGEIGEWLEVRHWISELHDLCKGFLSVCFSYIPRDKNRKADALAKDALMHQQNM 674
           + +  +N  +    E+   + E+  +   F S+ F +IPR  N  ADA AK +L    N+
Sbjct: 243 SIVDALNSNV-SLNEIFGLLVEIRSIRNRFRSISFQFIPRLVNSIADAAAKLSLCISGNI 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030479133.13.4e-12134.74uncharacterized protein LOC115696372 [Cannabis sativa][more]
XP_030497600.11.7e-12034.34uncharacterized protein LOC115713257 [Cannabis sativa][more]
XP_030502555.13.3e-11633.28uncharacterized protein LOC115717715 [Cannabis sativa][more]
XP_030508852.11.6e-11535.60uncharacterized protein LOC115723496 [Cannabis sativa][more]
XP_030509050.13.1e-11433.76uncharacterized protein LOC115723712 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
P0C2F61.2e-4727.44Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P932953.4e-3144.74Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A803QGT21.6e-12134.74Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PIB68.2e-12134.34Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PKJ22.9e-11833.33Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NM276.5e-11835.29Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803Q8J45.5e-11735.20Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.11.4e-5628.35Ribonuclease H-like superfamily protein [more]
ATMG00310.12.4e-3244.74RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G09510.12.8e-2823.82Ribonuclease H-like superfamily protein [more]
AT3G25270.11.5e-1323.38Ribonuclease H-like superfamily protein [more]
AT1G10000.16.8e-1126.33Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 350..440
e-value: 2.9E-18
score: 66.3
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 552..666
e-value: 9.1E-21
score: 73.9
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 569..668
score: 10.854897
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 540..674
e-value: 4.0E-18
score: 67.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 31..394
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 31..394
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 543..664
e-value: 6.18671E-20
score: 83.9028
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 540..675

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004473.1Lag0004473.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity