Lag0004432 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004432
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr6: 3801349 .. 3803819 (+)
RNA-Seq ExpressionLag0004432
SyntenyLag0004432
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGGCAAGGAGCTTTTAGAGATGGGTGTGCGTTGGCGAGTTGGGAATGGAGACCGGATAAAGATCTATGGATCGAACTGGATTCCAAAGGACACTAATTTGAAAGTCAGCTCACCGATATCCTTACCTTTAGATGCCACAGTGGCAGAACTGATGACGGCCTCGAGAGGGTGGGATCAAACGCTGTTGGAACAACACTTTAATGCCGCGAAGGTAAATCACATCTTGTCTATCCCCACTAGACAACATTTCCCCGTGGACTCAGTTATTTGGCATTATGAAAAATCTGGGCTCTTCTCAGTCAAAAGCGGATACAAGTTGGCTCAGTCGTTATCCTGGCGCGGGGGGCCTCGTCCTCTTCTCCCAACTCTATCCATGGCTGGTGGAAGGGTGTTGGAAGTTGATGATTCCAAGTAAGATAAAGGTGTTCCTTTGGAGAACGTGTCTGAATAGGTTGCCGACGATTGATAATCTGATAAGAAGGGGCATAGACCTGCTAGATGTATGCGTTTTCTGCAGGAGAAGGGGGGAATCTAGCATCCACCTTTTCTGGGAATGCAAATGGGCCAGATCAATCCTTCAAGAAGCAGGGTTTGAGGGGATTTTGGAGAGAGTCCGAGCAGGGTGTTGTCTACAACTCTGTAGGGACATTAAGGAAGGGATGGGGATGCGAGCTTCGAGGAGTTGGTGGTGTTATGGTGGGCAATGTGGTCGGCCCGGAATAAAGCTCGTTTTCAAGGGGCTGAAAGGCCGAGTGGGCTAGTGGAATGGGCTAAAGGGTATGTAATGGCTTTTCGTGAGGTTGGGAGGAGTAGGGAGGAAGGTCGAGTGACGATTTGTCGTGAGAGGATAGTGTGGCGGGCGCCAAGTAATGGCTGGTATAAGGTGAATTTTGATGCCTCTTTTCTGTCTGATGTGTCCAGGGCTGGTCTAGGGATAATTGTGAGAAACCCTATGGGCCAAGTGATGCTTTCAGCGACTTTCACTAAGGATAATGTGAGTGATGTCGACATGGTTGAGGGATATGCAGTTGTGAAAAGTCTGGAGCTCGTGAGAGATATGGGTTTCGGCCCATCAATTCTTGAGACGGACTCGAGTAGAGTTTGCCAGCTTCTTAATCGAGAGCGCGAGGATGCGTCTGAGCTAGGTATGACTATTGCAGATGCAATGATGGCTTTTCCTTCTTATTTGCAGGTATTGTTTGGTTTCACTCACCGTGAGGGGAATCAGGCGGCGCACCGATTGGCGAGGTTAGCAATTGGTCGAGGGAGGGATGAGGTATGGATGGAGACCTACCCTATGTGTCTGGAGGACGTAATCTTGAACGATATAGTGTACATGGAGGCATGAGGTGGGGGGCTACGCTATGCCAGTTCCGCTTTTACACCAACTGACGTGGAAATGCAAACAGGGAAGGTTTCTGTTTCTCTTTCCTTTGTGTTTGTTTTGCCTATTGCGGACTACATTGAGGAGATTGCATGTTTTTTCAAGGGGGTTTGGGTTGTATTTTGCATGATAGTGGATTAGACTGACGAGGGTAGTATATGAGCGCATGGGCTCTATTAATTCATGTTTTGCTTACTTGGTGCTATGAAGCTTGGTTGGAGGCCAAGCATCTGAGCATTGGACCTTTTTGTTTCTGGGATTAGTGGAAACTGTGGTGAGACTAATAGCTGAGTTCTGGGGTTTGAGTTATGAGCCATGATACTGCTGTTTGAGTACTCGTTCACTCTTTTTTTAGTGTATAGGTGAAGTTTGCCTGCTAGTCTAATTTTGGGGCCAGTTTATTGCTTTCTGATTGTCTTGCACCTTTTTGAGCACTTGGCATGTCCTCTAGGTAGACTTGAAGGTCTAGATCTGCTTTATGGGGTTCATAAATCTGTTGTCACTCCGAAAGGGGTAAGGTTTTGATTGGGAGTATCTGATTTCTGCAAGGTTGGGTGTTGTCTTGGCTTTGGGGAATTGAGAGGCTTGAAAAAAGGATAAGGAAAAGGAAAAGAGGAAAAGGAAAAGAAAAAGAAAAGAAAGAAAAGAAAAAGAAAAAAAAGATAGAAATGGAAAAGCGAAAATGCGAAATGGAAAGAAAGAAATTGAAAAGATTGAGTGGTTTGTGGCTGGGGGTTTATTTGGGGGTATATGCACACTTTTGTTTTCCTAAAAGCTTTTTTGTGTCATTGATTATACATAAGCATGGGGTAAAAAGAGCTACCTGCAGTGTGTTCAGTTAGGCCAACCAAGGGCTAAAACCTGGGTTAGGGGTCCCTGCTCTGTAGTGTGAAAGCTAGTGCCTCTCTTTTAGCATCAGTGTGCATTAGGATAAGGGAAAGGAAAATGAATCCTTTTCTGGTTCCTGGGTTTTGGGATAGCATGCTAAATCTGCTCCGATCGATCTGCCATGCGAAACCTTAGTTGCCAGTGTAGGGGAAAGATGGGTGGAGGAGCTCATAAATCTGGTTCTGGATCAGTAG

mRNA sequence

ATGTGGGGCAAGGAGCTTTTAGAGATGGGTGTGCGTTGGCGAGTTGGGAATGGAGACCGGATAAAGATCTATGGATCGAACTGGATTCCAAAGGACACTAATTTGAAAGTCAGCTCACCGATATCCTTACCTTTAGATGCCACAGTGGCAGAACTGATGACGGCCTCGAGAGGGTGGGATCAAACGCTGTTGGAACAACACTTTAATGCCGCGAAGGTAAATCACATCTTGTCTATCCCCACTAGACAACATTTCCCCGTGGACTCAGTTATTTGGCATTATGAAAAATCTGGGCTCTTCTCAGTCAAAAGCGGATACAAGTTGGCTCAGTCGTTATCCTGGCGCGGGGGGCCTCGTCCTCTTCTCCCAACTCTATCCATGGCTGGTGGAAGGGTGTTGGAAGTTGATGATTCCAAGGATGGGGATGCGAGCTTCGAGGAGTTGGTGGTGTTATGGTGGGCAATGTGGTCGGCCCGGAATAAAGCTCGTTTTCAAGGGGCTGAAAGGCCGAGTGGGCTAGTGGAATGGGCTAAAGGGTATGTAATGGCTTTTCGTGAGGTTGGGAGGAGTAGGGAGGAAGGTCGAGTGACGATTTGTCGTGAGAGGATAGTGTGGCGGGCGCCAAGTAATGGCTGGTATAAGGTGAATTTTGATGCCTCTTTTCTGTCTGATGTGTCCAGGGCTGGTCTAGGGATAATTGTGAGAAACCCTATGGGCCAAGTGATGCTTTCAGCGACTTTCACTAAGGATAATGTGAGTGATGTCGACATGGTTGAGGGATATGCAGTTGTGAAAAGTCTGGAGCTCGTGAGAGATATGGGTTTCGGCCCATCAATTCTTGAGACGGACTCGAGTAGAGTTTGCCAGCTTCTTAATCGAGAGCGCGAGGATGCGTCTGAGCTAGGTATGACTATTGCAGATGCAATGATGGCTTTTCCTTCTTATTTGCAGGTATTGTTTGGTTTCACTCACCGTGAGGGGAATCAGGCGGCGCACCGATTGGCGAGCTTGGTTGGAGGCCAAGCATCTGAGCATTGGACCTTTTTGTTTCTGGGATTAGTGGAAACTGTGGTGAGACTAATAGCTGAGTTCTGGGGTAGACTTGAAGGTCTAGATCTGCTTTATGGGGTTCATAAATCTGTTGTCACTCCGAAAGGGCATGCTAAATCTGCTCCGATCGATCTGCCATGCGAAACCTTAGTTGCCAGTGTAGGGGAAAGATGGGTGGAGGAGCTCATAAATCTGGTTCTGGATCAGTAG

Coding sequence (CDS)

ATGTGGGGCAAGGAGCTTTTAGAGATGGGTGTGCGTTGGCGAGTTGGGAATGGAGACCGGATAAAGATCTATGGATCGAACTGGATTCCAAAGGACACTAATTTGAAAGTCAGCTCACCGATATCCTTACCTTTAGATGCCACAGTGGCAGAACTGATGACGGCCTCGAGAGGGTGGGATCAAACGCTGTTGGAACAACACTTTAATGCCGCGAAGGTAAATCACATCTTGTCTATCCCCACTAGACAACATTTCCCCGTGGACTCAGTTATTTGGCATTATGAAAAATCTGGGCTCTTCTCAGTCAAAAGCGGATACAAGTTGGCTCAGTCGTTATCCTGGCGCGGGGGGCCTCGTCCTCTTCTCCCAACTCTATCCATGGCTGGTGGAAGGGTGTTGGAAGTTGATGATTCCAAGGATGGGGATGCGAGCTTCGAGGAGTTGGTGGTGTTATGGTGGGCAATGTGGTCGGCCCGGAATAAAGCTCGTTTTCAAGGGGCTGAAAGGCCGAGTGGGCTAGTGGAATGGGCTAAAGGGTATGTAATGGCTTTTCGTGAGGTTGGGAGGAGTAGGGAGGAAGGTCGAGTGACGATTTGTCGTGAGAGGATAGTGTGGCGGGCGCCAAGTAATGGCTGGTATAAGGTGAATTTTGATGCCTCTTTTCTGTCTGATGTGTCCAGGGCTGGTCTAGGGATAATTGTGAGAAACCCTATGGGCCAAGTGATGCTTTCAGCGACTTTCACTAAGGATAATGTGAGTGATGTCGACATGGTTGAGGGATATGCAGTTGTGAAAAGTCTGGAGCTCGTGAGAGATATGGGTTTCGGCCCATCAATTCTTGAGACGGACTCGAGTAGAGTTTGCCAGCTTCTTAATCGAGAGCGCGAGGATGCGTCTGAGCTAGGTATGACTATTGCAGATGCAATGATGGCTTTTCCTTCTTATTTGCAGGTATTGTTTGGTTTCACTCACCGTGAGGGGAATCAGGCGGCGCACCGATTGGCGAGCTTGGTTGGAGGCCAAGCATCTGAGCATTGGACCTTTTTGTTTCTGGGATTAGTGGAAACTGTGGTGAGACTAATAGCTGAGTTCTGGGGTAGACTTGAAGGTCTAGATCTGCTTTATGGGGTTCATAAATCTGTTGTCACTCCGAAAGGGCATGCTAAATCTGCTCCGATCGATCTGCCATGCGAAACCTTAGTTGCCAGTGTAGGGGAAAGATGGGTGGAGGAGCTCATAAATCTGGTTCTGGATCAGTAG

Protein sequence

MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWDQTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQSLSWRGGPRPLLPTLSMAGGRVLEVDDSKDGDASFEELVVLWWAMWSARNKARFQGAERPSGLVEWAKGYVMAFREVGRSREEGRVTICRERIVWRAPSNGWYKVNFDASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGPSILETDSSRVCQLLNREREDASELGMTIADAMMAFPSYLQVLFGFTHREGNQAAHRLASLVGGQASEHWTFLFLGLVETVVRLIAEFWGRLEGLDLLYGVHKSVVTPKGHAKSAPIDLPCETLVASVGERWVEELINLVLDQ
Homology
BLAST of Lag0004432 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 212.6 bits (540), Expect = 6.5e-51
Identity = 139/418 (33.25%), Postives = 190/418 (45.45%), Query Frame = 0

Query: 1    MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASR-GW 60
            +WG++LL+ G+RWR+GNGD + IYG NW+P    LK+ S   LPL + V+ L+     GW
Sbjct: 705  LWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGW 764

Query: 61   DQTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLA---------- 120
               ++   F   +   ILSIP  +    D +IW+YEK+G++SV+SGYK+A          
Sbjct: 765  QGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAP 824

Query: 121  ------QSLSWRGG------PRPL-----------LPTLSMAGGRVLEVD---------- 180
                  +   W  G      P  +           LPT      R +E+           
Sbjct: 825  SSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNG 884

Query: 181  ------------------DSKDG----------------DASFEELVVLWWAMWSARNKA 240
                              +SK G                 A FEEL V+ W +W+ RN  
Sbjct: 885  EDSIHLFWICKFAEALWINSKFGKLSPFLILRESHESLSKADFEELCVVIWGLWNQRNAR 944

Query: 241  RFQGAERP-----SGLVEWAKGYVMAFREVGRSREEGRVTICRERIVWRAPSNGWYKVNF 300
             F  + +        LVEWA  Y M FRE   +   GRVT   E I+W+ P  G YK+N 
Sbjct: 945  AFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAE-ILWQPPDEGIYKINT 1004

Query: 301  DASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGP 336
            DASFL+    AGLGII+ N  GQVM +AT   +N+  VDM E  A V+ L+L  ++G  P
Sbjct: 1005 DASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHP 1064

BLAST of Lag0004432 vs. NCBI nr
Match: XP_015388020.1 (uncharacterized protein LOC107177951 [Citrus sinensis])

HSP 1 Score: 172.9 bits (437), Expect = 5.7e-39
Identity = 101/341 (29.62%), Postives = 160/341 (46.92%), Query Frame = 0

Query: 1    MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWD 60
            +WG+E++  G+RWR+ +G  +K+Y S W+P+    K  SP SLPLD TV+ L+     W 
Sbjct: 688  LWGREVIHKGLRWRIRDGKLVKVYQSGWLPRPETFKPISPPSLPLDTTVSVLIDEDHCWR 747

Query: 61   QTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQSLSWRGGPRP 120
               + +HF+      IL IP  +   +D V+WHY+K G +SVKSGY+LA  + +   P  
Sbjct: 748  DDAIRKHFHQEDAAQILKIPLPRQPSLDQVLWHYDKKGNYSVKSGYQLALQMKFPNRP-- 807

Query: 121  LLPTLSMAGGRVLEVDDSKDGDASFEELVVLWWAMWSARNKARFQGAERPSGLVEWAKGY 180
                             SK+G +S+     +W+     +NK      E     V  A+  
Sbjct: 808  ---------------SSSKEGKSSWSS---IWYLQIPEKNK-----KEDSQLSVAGAEAI 867

Query: 181  VMAFREVGRSREEGRVTICRERIV----WRAPSNGWYKVNFDASFLSDVSRAGLGIIVRN 240
            V +++ +   + +    I  + +     W  P  GW+KVN DA+   D  R GLGI++RN
Sbjct: 868  VQSYKRIQMPQLQ---AISDQNLASQEHWNCPPAGWFKVNVDAAVKIDNQRVGLGIVIRN 927

Query: 241  PMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGPSILETDSSRVCQLLNRERE 300
              G+++ +A      +  +D  E  A    LE+    G  P I+ETDS  V  L++  + 
Sbjct: 928  SEGKLITAAIKPSKWLDRMDYAEAEATRFGLEVAEIAGCLPLIVETDSKEVSNLVSARKS 987

Query: 301  DASELGMTIADAMMAFPSYLQVLFGFTHREGNQAAHRLASL 338
              +E+   +++          V      R  N  AH LA L
Sbjct: 988  TKAEIFWIVSEVQETMERLKHVKLQHLPRTCNGFAHTLAKL 1000

BLAST of Lag0004432 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 172.6 bits (436), Expect = 7.4e-39
Identity = 116/421 (27.55%), Postives = 174/421 (41.33%), Query Frame = 0

Query: 1    MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWD 60
            +WG+++L  G RWR+GNG  + +YG+NWIP+ T  K  S  S+  D TVAEL+   + W 
Sbjct: 711  VWGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMGTDTTVAELIDEKQQWR 770

Query: 61   QTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQSLS------- 120
            + L+ QHF       I+ IP  +    D +IWHY+K G +SVKSGY++A  +        
Sbjct: 771  EDLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKSGYQVAMRIKFPEDPSC 830

Query: 121  ------------------------WRGGPRPLLPTLSMAGGR------------------ 180
                                    WR     LLPT      +                  
Sbjct: 831  SNHDQNLWRFIWKLAIPEKVKIFLWRAA-HDLLPTAENLWKKKVLQEPMCQSCHCHVETV 890

Query: 181  ---------------------------------VLEVDDSKDGDASFEELVVLWWAMWSA 240
                                             +L+    +       E+  L WA+W A
Sbjct: 891  SHALVECNRARKIWRYSNLAEELRGVYRCDIVWMLQFWPRQHAKVEGAEVAALLWAIWKA 950

Query: 241  RNKARFQG-AERPSGLVEWAKGYVMAFREVGRSREEGRVTICRER-IVWRAPSNGWYKVN 300
            RNK  F+G  E P  +V  A+  V +F+++ +     +     ER   W  P NGW KVN
Sbjct: 951  RNKWLFEGKKENPLRVVANAEAIVESFKKIRQPEMVYKTKGNAERQKQWSPPPNGWQKVN 1010

Query: 301  FDASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFG 338
             DA+   +   AGLG++VR+  G    +A  +      V M E  A+   L++       
Sbjct: 1011 VDAAVDVENQMAGLGVVVRDSDGNCRAAAIKSLRLPGSVAMAEATAMEWGLKVAEKAHIT 1070

BLAST of Lag0004432 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 169.1 bits (427), Expect = 8.2e-38
Identity = 113/426 (26.53%), Postives = 176/426 (41.31%), Query Frame = 0

Query: 1    MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWD 60
            MWG+++++ G+RWR+GNG +I I+  NW+P+    +   P+SLP+ + VA+L+ A   WD
Sbjct: 947  MWGRQVIKKGMRWRIGNGKKIAIFSDNWLPRPETFRPIFPLSLPVSSVVADLIKADNQWD 1006

Query: 61   QTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLA----------- 120
            +  L QHF       IL IP       D V+WHY+K G +SVKSGY+LA           
Sbjct: 1007 EIKLRQHFLDVDTAEILKIPLPAEKAEDEVLWHYDKRGNYSVKSGYQLALRSKFPDSTSC 1066

Query: 121  --------------------QSLSWRGGPRPLLPTLSMAGGRVLEVDDSKDGDASFEE-- 180
                                +   WR     L    ++   +V+E    K    S E   
Sbjct: 1067 TEASHKYWSALWTLELPEKLKIFMWRASNNLLPSAENLWKRKVVEEPTCKRCKLSVETIS 1126

Query: 181  ------------------------------------------------LVVLWWAMWSAR 240
                                                            +V L W+ W AR
Sbjct: 1127 HALLECKAARKIWLQSPFSAPRLEANSQDIFSTLQNMAKELRKSDLELMVALCWSAWYAR 1186

Query: 241  NKARFQGAE-RPSGLVEWAKGYVMAFREVGRSREEG-RVTICRERIVWRAPSNGWYKVNF 300
            NK  F G E  P      A+  + AF+ V + ++    ++I  ++  W  P    +KVN 
Sbjct: 1187 NKCIFDGRELNPIISAAKAESVLTAFQRVRKPQQSHISISIKEKQQEWLPPPQNVFKVNV 1246

Query: 301  DASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGP 344
            DA+F S    AG+G ++R+  G+++ +            + E  AV+  L+L R+     
Sbjct: 1247 DAAFNSKNLSAGVGAVIRDSNGKIVAAGVNQNLLKGSASLAEAEAVLWGLQLARNADVSS 1306

BLAST of Lag0004432 vs. NCBI nr
Match: XP_024033483.1 (uncharacterized protein LOC112095606 [Citrus clementina])

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-36
Identity = 111/381 (29.13%), Postives = 174/381 (45.67%), Query Frame = 0

Query: 13  WRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWDQTLLEQHFNAAK 72
           WR+G+G +I+I+  NWIPK +  K     SLP D  VAEL+     WD++ + +HFN   
Sbjct: 302 WRIGDGQKIQIHKDNWIPKPSTFKPIVRPSLPEDTLVAELINEENQWDESQIYRHFNRMD 361

Query: 73  VNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQSLSWRGGPRPLLPTLSMAGG-- 132
            + I+ IP  +    D++IWHY+K GL+SVKSGY+ A SL +     P +P  SM+G   
Sbjct: 362 ADKIVEIPLPRSPKEDTIIWHYDKKGLYSVKSGYQTALSLKF-----PAMPLSSMSGKNQ 421

Query: 133 --------------------------------------------RVLEVDDSKDGDASFE 192
                                                       R L V++        +
Sbjct: 422 WNIIWTLALPEKIRIFAWRAAKNILPSAENLWKRKIIQEPTCQVRKLGVENVFHALVECK 481

Query: 193 ELVVLWW-------AMWSARNKARFQG-AERPSGLVEWAKGYVMAFREVGRSREEGRVTI 252
               +W         M +A N+  F+G  E P  LV  A+  + A++ + +  E+ R+  
Sbjct: 482 AARKIWKLTLLENDIMQTAGNEMLFKGKRENPQALVAKAEAVMEAYKRI-QKPEQARMES 541

Query: 253 --CRERIVWRAPSNGWYKVNFDASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVD 312
              + + VW  P +G+ KVN DA+  S+   AGLG I+R+  G V+ +A        DV 
Sbjct: 542 QQVQAQQVWNPPQSGFVKVNVDAATSSEKHLAGLGAIIRDDRGNVIAAAIKISKFHGDVS 601

Query: 313 MVEGYAVVKSLELVRDMGFGPSILETDSSRVCQLLNREREDASELGMTIADAMMAFPSYL 338
             E  A+   L++ R+      I+E+D+  V  L+N ++   SE+   I++      ++ 
Sbjct: 602 FAEAEALEWGLQIARNASTKALIVESDAQSVVNLVNNKQGGNSEIFWIISEIQNLMRTFD 661

BLAST of Lag0004432 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.1e-51
Identity = 139/418 (33.25%), Postives = 190/418 (45.45%), Query Frame = 0

Query: 1    MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASR-GW 60
            +WG++LL+ G+RWR+GNGD + IYG NW+P    LK+ S   LPL + V+ L+     GW
Sbjct: 705  LWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGW 764

Query: 61   DQTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLA---------- 120
               ++   F   +   ILSIP  +    D +IW+YEK+G++SV+SGYK+A          
Sbjct: 765  QGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAP 824

Query: 121  ------QSLSWRGG------PRPL-----------LPTLSMAGGRVLEVD---------- 180
                  +   W  G      P  +           LPT      R +E+           
Sbjct: 825  SSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNG 884

Query: 181  ------------------DSKDG----------------DASFEELVVLWWAMWSARNKA 240
                              +SK G                 A FEEL V+ W +W+ RN  
Sbjct: 885  EDSIHLFWICKFAEALWINSKFGKLSPFLILRESHESLSKADFEELCVVIWGLWNQRNAR 944

Query: 241  RFQGAERP-----SGLVEWAKGYVMAFREVGRSREEGRVTICRERIVWRAPSNGWYKVNF 300
             F  + +        LVEWA  Y M FRE   +   GRVT   E I+W+ P  G YK+N 
Sbjct: 945  AFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAE-ILWQPPDEGIYKINT 1004

Query: 301  DASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGP 336
            DASFL+    AGLGII+ N  GQVM +AT   +N+  VDM E  A V+ L+L  ++G  P
Sbjct: 1005 DASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHP 1064

BLAST of Lag0004432 vs. ExPASy TrEMBL
Match: A0A2N9HME5 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40773 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.6e-37
Identity = 104/340 (30.59%), Postives = 161/340 (47.35%), Query Frame = 0

Query: 4   KELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPI-SLPLDATVAELMTASRG-WDQ 63
           + +LE G +WR+GNG  I I    WIP   N K+  PI ++  DA VAEL+ + RG W +
Sbjct: 345 RHVLEQGSKWRIGNGKSISICQDRWIPDQENGKIPYPIAAVRSDAVVAELIDSDRGVWKE 404

Query: 64  TLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQSLSWRGGPRPL 123
            L+  +F+   V HIL  P     P D +IW    S + +    Y  A+  S        
Sbjct: 405 DLVRSYFSPVDVEHILHYPLHSSQPADVLIWWGTSSAILASSLVYATAKENS-------- 464

Query: 124 LPTLSMAGGRVLEVD-----DSKDGDASFEELVVLWWAMWSARNKARFQGAER-PSGLVE 183
                M G + + +D         G+A+ +E+ V+ W +W+ RN A F+   + PS +  
Sbjct: 465 --AFCMEGRQGI-IDWFWGIWKLKGEAALQEVAVVSWGLWNVRNSAIFRDLIKIPSKVYV 524

Query: 184 WAKGYVMAFREVGRSREEGRVTICRERIVWRAPSNGWYKVNFDASFLSDVSRAGLGIIVR 243
            A  YV  ++E     E  R+ I   R  W AP  GWYKVN D +  SD+ + G+G+++R
Sbjct: 525 DAVQYVKVYQEAQVQSECSRLPISTGRKGWTAPREGWYKVNMDGAVFSDIKKVGVGVVIR 584

Query: 244 NPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGPSILETDSSRVCQLLNRER 303
           N +G+ + + +   D   D    E +A ++++E   ++     I E D  +V + L+   
Sbjct: 585 NELGEFLGAMSELLDFGLDATDAEAWAALRAVEFTAEVCPFNIIFEGDCVQVTKALSSSE 644

Query: 304 EDASELGMTIADAMMAFPSYLQVLFGFTHREGNQAAHRLA 336
            D S++G   A A                REGN  AH LA
Sbjct: 645 FDCSKVGHLYAIARSKLDLCRNHSVNHVFREGNSVAHCLA 673

BLAST of Lag0004432 vs. ExPASy TrEMBL
Match: A0A2N9IUU4 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56027 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 9.1e-35
Identity = 107/390 (27.44%), Postives = 167/390 (42.82%), Query Frame = 0

Query: 4   KELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPI-SLPLDATVAELMTASRG-WDQ 63
           + +LE G +WRVGNG+ I I    W+P  TN  +  P+  LP +A VA+L+   RG W +
Sbjct: 20  RSVLEQGSKWRVGNGECISIRKDRWVPDQTNGVIHYPLEGLPQEAMVADLIDPGRGVWKE 79

Query: 64  TLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLA------------ 123
            ++ + F+   V  IL  P     P D++IW    SG FSVKS Y  A            
Sbjct: 80  EVVRRCFSPLDVASILQYPLLPSLPKDALIWWGTPSGNFSVKSAYHTAFLKVQIQGNPES 139

Query: 124 ----------------------QSLSWRGGPRPLLPTLSMAGGRVL-------------- 183
                                 QS  WR   R +LPT      R +              
Sbjct: 140 SNSKELQLFWKAVWALKLPRKIQSFVWRLS-RNILPTRDNLVKRNISSPMECPICGAASE 199

Query: 184 -------EVDDSKDGDASFEELVVLWWAMWSARNKARFQGAER-PSGLVEWAKGYVMAFR 243
                  +   ++ G+ + + + V+ W++W+ RN A F+   + P+ +   A  Y+  F 
Sbjct: 200 TTKHIFGQCIYARKGEMALKIVTVVSWSLWNVRNSAVFRDVVKIPAKVFPDAMKYIQEFT 259

Query: 244 EVGRSREEGRVTICRERIVWRAPSNGWYKVNFDASFLSDVSRAGLGIIVRNPMGQVMLSA 303
           E           I R +IVW AP  GWYKVN D +  +D+ + G+G+++RN  G+ + + 
Sbjct: 260 EANALSVSFVPPIARNKIVWSAPLEGWYKVNMDGAVFADLGKVGVGVVIRNEQGEFLGAM 319

Query: 304 TFTKDNVSDVDMVEGYAVVKSLELVRDMGFGPSILETDSSRVCQLLNREREDASELGMTI 336
               D   D    E  A ++++E   ++     + E D  +V + L     DA+ +G   
Sbjct: 320 CELLDVGLDATDGEALAALRAIEFAAEVCPFSMLFEGDCLQVIRALQSPEYDAARVGHIY 379

BLAST of Lag0004432 vs. ExPASy TrEMBL
Match: A0A2H5Q972 (Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_207140 PE=4 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.0e-34
Identity = 109/420 (25.95%), Postives = 176/420 (41.90%), Query Frame = 0

Query: 1   MWGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWD 60
           +WG++++  G RWR+GNG  + I+ +NWIPK    K     +LP  A V+EL+     WD
Sbjct: 23  LWGRQIICAGSRWRIGNGQDVHIHKANWIPKPFTFKPVIKPTLPSKALVSELINGENCWD 82

Query: 61  QTLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQSLS------- 120
           + L+ +HF+    + I  IP  +    D +IWH+ KSG ++VKSGY+ A ++        
Sbjct: 83  EELIYRHFDKMDADVITQIPLPRRSREDELIWHFGKSGQYTVKSGYQTALNIRFPAMPSS 142

Query: 121 ------------------------WRGGPRPLLPT------------------------- 180
                                   WR   + LLP+                         
Sbjct: 143 SEFSKNEWNIIWSLALPKKIRIFVWRAA-KNLLPSDENLWKRKIVQEPTCQLCKMGIENV 202

Query: 181 ----------------------LSMAGGR----VLEVDDSKDGDASFEELVVLWWAMWSA 240
                                 +  A G+    +L        +A  +    + WA W+A
Sbjct: 203 FHALVDCKAAKKVWRLSLFDNDIQAAPGQDILSLLHGVKRMRSNADVDLFAAMLWAKWNA 262

Query: 241 RNKARFQG-AERPSGLVEWAKGYVMAFREVGRSREEGRVTICRERIVWRAPSNGWYKVNF 300
           RN+  F+G  E P  +V  A+  + A++ V  S ++        ++ W  P  G+ K+N 
Sbjct: 263 RNQWLFKGKRENPQSVVAKAEAVMEAYKRVQPSADK------VAQLGWNPPQEGFVKINT 322

Query: 301 DASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVKSLELVRDMGFGP 338
           DA+  S+ + AGLG ++R+  GQV  +A         V   E  A+   L++ +D     
Sbjct: 323 DAATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKD 382

BLAST of Lag0004432 vs. ExPASy TrEMBL
Match: A0A6J5TGK7 (CCHC-type domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS2532 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.7e-34
Identity = 114/383 (29.77%), Postives = 170/383 (44.39%), Query Frame = 0

Query: 2    WGKELLEMGVRWRVGNGDRIKIYGSNWIPKDTNLKVSSPISLPLDATVAELMTASRGWDQ 61
            WGKELL  G+RWRVGNG+ I++Y + W+P  +  K+ S   LPL   V +L T+S  W+ 
Sbjct: 1306 WGKELLNKGLRWRVGNGESIQVYTNKWLPVPSFFKIMSAPQLPLSTLVCDLFTSSGQWNV 1365

Query: 62   TLLEQHFNAAKVNHILSIPTRQHFPVDSVIWHYEKSGLFSVKSGYKLAQ----------- 121
             LL+  F   +V  IL IP       D +IWHYE++G++SVKSGY+L +           
Sbjct: 1366 PLLKDIFWDQEVEAILQIPLVSLASHDCLIWHYERNGMYSVKSGYQLTRLEKDIMCGESS 1425

Query: 122  ------SLSWRGGPRPLLPT-----------LSMAGGRVLEVDDSKDGDASFEEL----- 181
                  S  W+      +P             +  GG V EV        SF EL     
Sbjct: 1426 AGVNLSSRFWKKIWALKIPNKIKFFLWRRCGATQLGGNVCEVSRVN----SFRELWYAVQ 1485

Query: 182  -----------VVLWWAMWSARNKARFQG-AERPSGLVEWAKGYVMAFREVGRS--REEG 241
                         L W +W+ R+   F+  +E  + L+         F +V  S     G
Sbjct: 1486 ISSGGEEQGLFAYLRWGLWNWRDSLIFEAKSETATQLLHRMHTLAQEFFDVNNSSHTSHG 1545

Query: 242  RVTICRERI-VWRAPSNGWYKVNFDASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVS 301
            R +  +  + VWR P+ G YK+N D +  S  S  G+G++VRN  G  M +         
Sbjct: 1546 RQSSPQASLHVWRPPAVGIYKINVDRALKSGDSIRGVGVVVRNENGDFMAACVRRIQASY 1605

Query: 302  DVDMVEGYAVVKSLELVRDMGFGPSILETDSSR-VCQLLNREREDASELGMTIADAMMAF 336
                +E  A ++ L    DMGF  ++LE D+   +  +L+ E       G+ I +     
Sbjct: 1606 GARQMELMATIEGLRFAIDMGFTDAVLEMDAQDCIHSILSTECNGVD--GLLIEEVKSLL 1665

BLAST of Lag0004432 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 1.4e-11
Identity = 54/190 (28.42%), Postives = 88/190 (46.32%), Query Frame = 0

Query: 151 LWWAMWSARNKARFQGAERPSGLVEWAKGYVMAFREVGRSRE-EGRVTICR-ER---IVW 210
           L W +W +RN+  F+G E  +   E  +  +  F E    RE EG+ +  + ER   + W
Sbjct: 80  LLWRLWKSRNELMFKGKEYDA--PEVLRRAMEDFEEWSTRRELEGKASGPQVERNLSVQW 139

Query: 211 RAPSNGWYKVNFDASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVK 270
           +AP   W K N DA++  +  R G+G I+RN  G V+           +V   E  A+  
Sbjct: 140 KAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRW 199

Query: 271 SLELVRDMGFGPSILETDSSRVCQLLNREREDASELGMTIADAMMAFPSYLQVLFGFTHR 330
           ++  +    +   I E+D+  +  LLN + +    L   + D       + +V F FT R
Sbjct: 200 AVLTMSRFNYKRIIFESDAQALVNLLNSD-DFWPTLQPALEDIQQLLHHFEEVKFEFTPR 259

Query: 331 EGNQAAHRLA 336
            GN+ A R+A
Sbjct: 260 GGNKVADRIA 266

BLAST of Lag0004432 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.5e-08
Identity = 49/190 (25.79%), Postives = 82/190 (43.16%), Query Frame = 0

Query: 151 LW--WAMWSARNKARFQGAERP-SGLVEWAKGYVMAFRE-VGRSREEGRVTICRERIV-W 210
           LW  W +W++RN   FQ    P    ++ A+  +  + E V  S      T  R  I  W
Sbjct: 444 LWILWRLWNSRNILYFQRRHVPWETTLQLAQMDLQEWNEAVTPSHINSHGTTIRSHIPNW 503

Query: 211 RAPSNGWYKVNFDASFLSDVSRAGLGIIVRNPMGQVMLSATFTKDNVSDVDMVEGYAVVK 270
           R P+NGW K N D SF++       G IVR+  G+   +     + + +    E  A++ 
Sbjct: 504 RRPTNGWVKCNVDGSFINGNIPGKGGWIVRDHNGRYEFAGQAIGNCIDNALECEFQALLM 563

Query: 271 SLELVRDMGFGPSILETDSSRVCQLLNREREDASELGMTIADAMMAFPSYLQVLFGFTHR 330
           +++     G+     E D+  +  L+N  +         I D +     + Q  F +T+R
Sbjct: 564 AMQHCWSKGYRRICFEGDNKEMFDLINGLKMHFGAFNW-IRDILWWASKFEQTQFQWTNR 623

Query: 331 EGNQAAHRLA 336
           + N+ A  LA
Sbjct: 624 QNNKPADILA 632

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150918.16.5e-5133.25uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_015388020.15.7e-3929.62uncharacterized protein LOC107177951 [Citrus sinensis][more]
XP_024037590.17.4e-3927.55uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_024950112.18.2e-3826.53uncharacterized protein LOC112496847 [Citrus sinensis][more]
XP_024033483.14.5e-3629.13uncharacterized protein LOC112095606 [Citrus clementina][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DAR43.1e-5133.25uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A2N9HME52.6e-3730.59Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9IUU49.1e-3527.44Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56027 PE=4 SV=1[more]
A0A2H5Q9722.0e-3425.95Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_207140 PE=4... [more]
A0A6J5TGK72.7e-3429.77CCHC-type domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS... [more]
Match NameE-valueIdentityDescription
AT2G34320.11.4e-1128.42Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G33160.12.5e-0825.79glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 216..336
e-value: 9.3E-21
score: 73.9
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 212..338
e-value: 3.9E-12
score: 48.3
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 6..297
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 215..335
e-value: 1.84377E-13
score: 64.6428
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 211..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004432.1Lag0004432.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity