Lag0004403 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004403
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionabscisic acid 8'-hydroxylase 1
Locationchr6: 3550269 .. 3552172 (-)
RNA-Seq ExpressionLag0004403
SyntenyLag0004403
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTGAAAGGAGTTGACGTTGTTCGTTTCGTGGTGGAGGAGGTGACAGTGGGAAGGCGTGGTGGAGGAGATGGCGGTGGAAGGAAAATAATCGTGGTGGAGGACGTTGTTCGTTTCGTGCTTGAGAGAGAGAGAAAAGGGAAAGAAAACAACGTACTGGATGAAAGACTGAAACCCTAAAAGTTCAATTTATATCAAAACTGATAACCAAATCGGTTCGGTTCGGGCTAGATAAATTTAAAAATTGAACCGAACCGACTCGATCCAGTTCGATTTATCGGTTAGGTTTGGTTTCTTTCATCCCTACTTAAACTTTCAAGTTTGTTCTATTTTAGTTTTAGAACTTTCAAAATATCTACACAATCTTAAAACTTTAAAAAGTACTTGTTTTGAACTTTTATATCTTATGTTCTTCTAAATACTCGCTCACATCTTGAGCACGTGTTGAATGCATGCTCATGTTAATATCTTAATATACATACTCAAAATCATGTTACTATCAATTAATTAAAAGAAAAATATACTGATTGAATGTTTAGAAACTAAAATTGAACAAATCTGAAAACTTGGAAAACAAATTCAATACCAAAATAGATTTAAACTTTTTTAAAAAAATTATTTAGAAGTTATTTAAAAACTATTTTGTCTTGTTTTTAGAAAGATATGAAATGAAAATAAGTAGTATTATTTTGGATGGACAAGACATGATTGATGGGTAAAATGTTTTGGAGACAGGTTACAAAATCAAGAAAGGTTGGAATATTAACATTGATGTAAGATCACTACACTCAGATCCTTCTGTCTACAACCATCCTACCAACTTCAATCCTTCCAGATTTGATGTAAGATATATTTCAGAATAACTTTTTTTTTATTTAATCTTTACATTATTAGTGAGAAATAAATCAATCAGAGAATTATATTGTCCAATATGGAGGTCTTCATGATCATCAATAACAATTAGTCTTTAAATAAAATACTTTGGTCATTTTCCTATGCATGAAAAAAATGATTATACCAAAATTTATTTTATACCTTACCTCTATATTCAAAAGTGATTCTATCAGTCATAAATATATTTTTTCTTCTTTTGATTACATATTTTGAAGATTCTTTTTATTGATCACTTTAAAATATAATCTTTCTATCAAGTGAGTGTTTATTTTTTTCCTTCAAAATAAGATTTTAAATTAATCTAATTTTAAGTTGTTTTTATTTATTAAGATTTTTAAAAAAAAAAAAACACTTTTGGTCGTCATTTAAAATCACTCTAGAAGTTTAAATATTAAAATTTTCAAAAAAAAAATATATAGAAAATTTGATTTTGTTTTAAAAAGTTATTCCAAACACATTGTAAAAGTGATTGTTTTCACCTTCTCATCTATAATTATAACTTCTTTTTTTTTTGGAGTTGAGGGGTTCGCTCACTTTGACCACATTAACCGACTTTTGAAAATCGTAACACCATAATGTATTTTTAAATACAAATTCTTTGATTAAAAAAGTGTAAATTTTTTTAATTTTTTTAGATGAAGAGTTTGGTTAAATTTGGTGTAGGAAGAAACAAAGCCCTATAGTTTTCTAGCTTTTGGAATGGGAGGGAGGCAATGTCTTGGAATGAATCTAGCCAAAGCCATGATGCTTGTCTTTCTTCACCGCTTGGTGACTTCTTACAGGTGACAGACCAATCATATCATTTTTTCCAAATTTTCTATTTCCCTCTGCATTACTAATAAATATTTGTTCCTCTTTAGAACACTAATTAATTAATTGATTTAATTGATTGATTAAATTTATGTAGATGGAAGGTGGTCGACTCGGACTCTAGCATTGAGAAGTGGGCACTTTTTTCCAAACTAAAGAGCGGTTGTCCGATTATCATCACACGAATTCAATCTTAA

mRNA sequence

ATGGCAGTGAAAGGAGTTGACGTTGTTCGTTTCGTGGTGGAGGAGGTGACAGTGGGAAGGCGTGGTGGAGGAGATGGCGGTGGAAGGAAAATAATCGTGGTGGAGGACGTTGTTCGTTACAAAATCAAGAAAGGTTGGAATATTAACATTGATGTAAGATCACTACACTCAGATCCTTCTGTCTACAACCATCCTACCAACTTCAATCCTTCCAGATTTGATGAAGAAACAAAGCCCTATAGTTTTCTAGCTTTTGGAATGGGAGGGAGGCAATGTCTTGGAATGAATCTAGCCAAAGCCATGATGCTTGTCTTTCTTCACCGCTTGGTGACTTCTTACAGATGGAAGGTGGTCGACTCGGACTCTAGCATTGAGAAGTGGGCACTTTTTTCCAAACTAAAGAGCGGTTGTCCGATTATCATCACACGAATTCAATCTTAA

Coding sequence (CDS)

ATGGCAGTGAAAGGAGTTGACGTTGTTCGTTTCGTGGTGGAGGAGGTGACAGTGGGAAGGCGTGGTGGAGGAGATGGCGGTGGAAGGAAAATAATCGTGGTGGAGGACGTTGTTCGTTACAAAATCAAGAAAGGTTGGAATATTAACATTGATGTAAGATCACTACACTCAGATCCTTCTGTCTACAACCATCCTACCAACTTCAATCCTTCCAGATTTGATGAAGAAACAAAGCCCTATAGTTTTCTAGCTTTTGGAATGGGAGGGAGGCAATGTCTTGGAATGAATCTAGCCAAAGCCATGATGCTTGTCTTTCTTCACCGCTTGGTGACTTCTTACAGATGGAAGGTGGTCGACTCGGACTCTAGCATTGAGAAGTGGGCACTTTTTTCCAAACTAAAGAGCGGTTGTCCGATTATCATCACACGAATTCAATCTTAA

Protein sequence

MAVKGVDVVRFVVEEVTVGRRGGGDGGGRKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMGGRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRIQS
Homology
[Close]
BLAST of Lag0004403 vs. NCBI nr
Match: KAG6585579.1 (Abscisic acid 8'-hydroxylase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 207.2 bits (526), Expect = 9.5e-50
Identity = 95/118 (80.51%), Postives = 105/118 (88.98%), Query Frame = 0 

Query: 29  RKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMG 88
           R ++    +  YK+ KGWN+NIDVRSLHS PS+Y+HP  FNPSRFDEE KPYSFLAFGMG
Sbjct: 433 RLVLQDSQIQGYKVNKGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMG 492

Query: 89  GRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRIQS 147
           GRQCLGMNLAKAMMLVFLHRLVTSYRWKV+DSD SIEKWALFSKLKSGCPII+TRI+S
Sbjct: 493 GRQCLGMNLAKAMMLVFLHRLVTSYRWKVIDSDPSIEKWALFSKLKSGCPIIVTRIES 550

[Close]
BLAST of Lag0004403 vs. NCBI nr
Match: XP_023537699.1 (abscisic acid 8'-hydroxylase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537700.1 abscisic acid 8'-hydroxylase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537701.1 abscisic acid 8'-hydroxylase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537702.1 abscisic acid 8'-hydroxylase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 94/118 (79.66%), Postives = 105/118 (88.98%), Query Frame = 0 

Query: 29  RKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMG 88
           R ++    +  YK+ KGWN+NIDVR+LHS PS+Y+HP  FNPSRFDEE KPYSFLAFGMG
Sbjct: 358 RLVLQDSQIQGYKVNKGWNVNIDVRALHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMG 417

Query: 89  GRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRIQS 147
           GRQCLGMNLAKAMMLVFLHRLVTSYRWKV+DSD SIEKWALFSKLKSGCPII+TRI+S
Sbjct: 418 GRQCLGMNLAKAMMLVFLHRLVTSYRWKVIDSDPSIEKWALFSKLKSGCPIIVTRIES 475

[Close]
BLAST of Lag0004403 vs. NCBI nr
Match: XP_023537703.1 (abscisic acid 8'-hydroxylase 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 94/118 (79.66%), Postives = 105/118 (88.98%), Query Frame = 0 

Query: 29  RKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMG 88
           R ++    +  YK+ KGWN+NIDVR+LHS PS+Y+HP  FNPSRFDEE KPYSFLAFGMG
Sbjct: 310 RLVLQDSQIQGYKVNKGWNVNIDVRALHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMG 369

Query: 89  GRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRIQS 147
           GRQCLGMNLAKAMMLVFLHRLVTSYRWKV+DSD SIEKWALFSKLKSGCPII+TRI+S
Sbjct: 370 GRQCLGMNLAKAMMLVFLHRLVTSYRWKVIDSDPSIEKWALFSKLKSGCPIIVTRIES 427

[Close]
BLAST of Lag0004403 vs. NCBI nr
Match: XP_022951698.1 (abscisic acid 8'-hydroxylase 1 [Cucurbita moschata])

HSP 1 Score: 205.7 bits (522), Expect = 2.8e-49
Identity = 94/118 (79.66%), Postives = 105/118 (88.98%), Query Frame = 0 

Query: 29  RKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMG 88
           R ++    +  YK+ +GWN+NIDVRSLHS PS+Y+HP  FNPSRFDEE KPYSFLAFGMG
Sbjct: 371 RLVLQDSQIQGYKVNEGWNVNIDVRSLHSHPSIYHHPLIFNPSRFDEEAKPYSFLAFGMG 430

Query: 89  GRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRIQS 147
           GRQCLGMNLAKAMMLVFLHRLVTSYRWKV+DSD SIEKWALFSKLKSGCPII+TRI+S
Sbjct: 431 GRQCLGMNLAKAMMLVFLHRLVTSYRWKVIDSDPSIEKWALFSKLKSGCPIIVTRIES 488

[Close]
BLAST of Lag0004403 vs. NCBI nr
Match: XP_023002298.1 (abscisic acid 8'-hydroxylase 1 isoform X3 [Cucurbita maxima] >XP_023002299.1 abscisic acid 8'-hydroxylase 1 isoform X3 [Cucurbita maxima] >XP_023002300.1 abscisic acid 8'-hydroxylase 1 isoform X3 [Cucurbita maxima] >XP_023002301.1 abscisic acid 8'-hydroxylase 1 isoform X3 [Cucurbita maxima] >XP_023002302.1 abscisic acid 8'-hydroxylase 1 isoform X3 [Cucurbita maxima])

HSP 1 Score: 202.6 bits (514), Expect = 2.3e-48
Identity = 93/118 (78.81%), Postives = 105/118 (88.98%), Query Frame = 0 

Query: 29  RKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMG 88
           R ++    +  YK+ KGWN+NIDVRSLHS PS+++HPT F+PSRF EE KPYSFLAFGMG
Sbjct: 357 RLVLQDSQIQGYKVNKGWNVNIDVRSLHSHPSIFHHPTIFDPSRFYEEAKPYSFLAFGMG 416

Query: 89  GRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRIQS 147
           GRQCLGMNLAKAMMLVFLHRLVTSYRWKV+DSD SIEKWALFSKLKSGCPII+TRI+S
Sbjct: 417 GRQCLGMNLAKAMMLVFLHRLVTSYRWKVIDSDPSIEKWALFSKLKSGCPIIVTRIES 474

[Close]
BLAST of Lag0004403 vs. ExPASy Swiss-Prot
Match: O81077 (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.2e-16
Identity = 42/105 (40.00%), Postives = 58/105 (55.24%), Query Frame = 0 

Query: 40  YKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMGGRQCLGMNLAK 99
           Y I KGW +    R +H     +  P  F+PSRF+   KPY+++ FG G   C G  LAK
Sbjct: 379 YLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAK 438

Query: 100 AMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITRI 145
             ML+ LH L TS+RW+V+  +  I+ +  F   K G PI +T I
Sbjct: 439 LEMLILLHHLTTSFRWEVIGDEEGIQ-YGPFPVPKKGLPIRVTPI 482

[Close]
BLAST of Lag0004403 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 9.3e-16
Identity = 41/115 (35.65%), Postives = 67/115 (58.26%), Query Frame = 0 

Query: 29  RKIIVVEDVVRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMG 88
           R+ +V  +   Y I KGW +    R++H +P  +++P  F+PSRF+   KP +F+ FG G
Sbjct: 352 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSG 411

Query: 89  GRQCLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITR 144
              C G  LAK  +L+FLH LV+++RW+V   +  I+ ++ F   ++G P    R
Sbjct: 412 VHACPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQ-YSPFPIPQNGLPATFRR 465

[Close]
BLAST of Lag0004403 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = 0 

Query: 34  VEDV--VRYKIKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMGGRQ 93
           VEDV    Y I KGW +    R++H    ++++P  F+PSRF+   KP +F+ FG G   
Sbjct: 351 VEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHS 410

Query: 94  CLGMNLAKAMMLVFLHRLVTSYRWKVVDSDSSIEKWALFSKLKSGCPIIITR 144
           C G  LAK  M + +H L T Y W +V +   I+ +  F+  ++G PI++ R
Sbjct: 411 CPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQ-YGPFALPQNGLPIVLAR 461

[Close]
BLAST of Lag0004403 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 40/91 (43.96%), Postives = 54/91 (59.34%), Query Frame = 0 

Query: 38  VRYK---IKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMGGRQCLG 97
           V YK   I KGW +    R++H +P  +  P  F+PSRF    +P +FL FG G   C G
Sbjct: 380 VEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPG 439

Query: 98  MNLAKAMMLVFLHRLVTSYRWKVVDSDSSIE 126
             LAK  MLV +HRLVT+YRW++V +   +E
Sbjct: 440 NELAKLEMLVLVHRLVTAYRWEIVGASDEVE 470

[Close]
BLAST of Lag0004403 vs. ExPASy Swiss-Prot
Match: Q6ZDE3 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 40/91 (43.96%), Postives = 54/91 (59.34%), Query Frame = 0 

Query: 38  VRYK---IKKGWNINIDVRSLHSDPSVYNHPTNFNPSRFDEETKPYSFLAFGMGGRQCLG 97
           V YK   I KGW +    R++H +P  +  P  F+PSRF    +P +FL FG G   C G
Sbjct: 384 VEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPG 443

Query: 98  MNLAKAMMLVFLHRLVTSYRWKVVDSDSSIE 126
             LAK  MLV +HRLVT+YRW++V +   +E
Sbjct: 444 NELAKLEMLVLVHRLVTAYRWEIVGASDEVE 474

The following BLAST results are available for this feature:
BLAST of Lag0004403 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6585579.19.5e-5080.51Abscisic acid 8'-hydroxylase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023537699.12.1e-4979.66abscisic acid 8'-hydroxylase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_02353... [more]
XP_023537703.12.1e-4979.66abscisic acid 8'-hydroxylase 2 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022951698.12.8e-4979.66abscisic acid 8'-hydroxylase 1 [Cucurbita moschata][more]
XP_023002298.12.3e-4878.81abscisic acid 8'-hydroxylase 1 isoform X3 [Cucurbita maxima] >XP_023002299.1 abs... [more]
BLAST of Lag0004403 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O810774.2e-1640.00Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 ... [more]
Q9LJK29.3e-1635.65Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
Q949P12.7e-1536.61Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ... [more]
Q09J782.7e-1543.96Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q6ZDE32.7e-1543.96Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 51..75
score: 30.45
coord: 82..92
score: 49.77
coord: 92..115
score: 27.41
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..121
e-value: 2.3E-25
score: 89.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 5..144
e-value: 8.7E-28
score: 99.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 9..143
NoneNo IPR availablePANTHERPTHR24286:SF189CYTOCHROME P450, FAMILY 722, SUBFAMILY A, POLYPEPTIDE 1coord: 37..142
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 37..142
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 85..94

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004403.1Lag0004403.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen