Lag0004310 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004310
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DPH4 homolog
Location	chr6: 2843622 .. 2844164 (+)
RNA-Seq Expression	Lag0004310
Synteny	Lag0004310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCAAGCAGTGGCTCTATTGGTGAAACTTATTATGATGTACTGTCTTTGAGAGAAGATGCCAGCTATGACGAAATTCGAGCCAGCTACCGGTCTGCCCTCCTTAATTTCCACCCTGATAAGTTACAATCTATGTGCCAGAAATCTCATCCAAATGACATCAAGGGAGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTCGGCAGCTCAACGTCTCGTGCAGCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGGTGCAGAGAGCATAAGCTTAGAGGACATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTCTTTTATCAGTGCCGCTGTGGTGATTACTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGTTCTTGTTCTTTGAAAGTTCGTTTGCTAATTGATTCGAATTCAAGTGTTTCGTTTGATTGTTGA

mRNA sequence

ATGAGTTCAAGCAGTGGCTCTATTGGTGAAACTTATTATGATGTACTGTCTTTGAGAGAAGATGCCAGCTATGACGAAATTCGAGCCAGCTACCGGTCTGCCCTCCTTAATTTCCACCCTGATAAGTTACAATCTATGTGCCAGAAATCTCATCCAAATGACATCAAGGGAGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTCGGCAGCTCAACGTCTCGTGCAGCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGGTGCAGAGAGCATAAGCTTAGAGGACATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTCTTTTATCAGTGCCGCTGTGGTGATTACTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGTTCTTGTTCTTTGAAAGTTCGTTTGCTAATTGATTCGAATTCAAGTGTTTCGTTTGATTGTTGA

Coding sequence (CDS)

ATGAGTTCAAGCAGTGGCTCTATTGGTGAAACTTATTATGATGTACTGTCTTTGAGAGAAGATGCCAGCTATGACGAAATTCGAGCCAGCTACCGGTCTGCCCTCCTTAATTTCCACCCTGATAAGTTACAATCTATGTGCCAGAAATCTCATCCAAATGACATCAAGGGAGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTCGGCAGCTCAACGTCTCGTGCAGCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGGTGCAGAGAGCATAAGCTTAGAGGACATGGTTGTGGAAGATAAAGGTGAAGTTGTAGAACTCTTTTATCAGTGCCGCTGTGGTGATTACTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGTTCTTGTTCTTTGAAAGTTCGTTTGCTAATTGATTCGAATTCAAGTGTTTCGTTTGATTGTTGA

Protein sequence

MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERYFKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSFDC

Homology

[Close]

BLAST of Lag0004310 vs. NCBI nr
Match: XP_038884890.1 (DPH4 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 334.3 bits (856), Expect = 6.3e-88
Identity = 168/179 (93.85%), Postives = 174/179 (97.21%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERY 60
           MSSSSGSIGETYYDVLSL+EDASYDEIRASYRSALLNFHPDKLQ+MC KSHP+DI  ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSS SRA+YDRELQAAKGDA+GAESISLEDMVVEDKGEVVELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSFD 180
           YFFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVS D
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 179

[Close]

BLAST of Lag0004310 vs. NCBI nr
Match: XP_008444636.1 (PREDICTED: DPH4 homolog [Cucumis melo])

HSP 1 Score: 332.0 bits (850), Expect = 3.1e-87
Identity = 165/180 (91.67%), Postives = 175/180 (97.22%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERY 60
           MSSSSG+IGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQ+MC+KSHP+DIKGERY
Sbjct: 1   MSSSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGS  SRA+YDRELQAAKGDA+GAESISLEDMVVEDKGEVVELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSFDC 180
            FFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKV L+ID+NSSVS DC
Sbjct: 121 CFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTNSSVSIDC 180

[Close]

BLAST of Lag0004310 vs. NCBI nr
Match: XP_023001946.1 (DPH4 homolog isoform X2 [Cucurbita maxima])

HSP 1 Score: 332.0 bits (850), Expect = 3.1e-87
Identity = 165/180 (91.67%), Postives = 172/180 (95.56%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERY 60
           MSSSSGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQ+MCQKSHPND+ GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKG+AMGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSFDC 180
           YFFIDSGELEEMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VS DC
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSIDC 180

[Close]

BLAST of Lag0004310 vs. NCBI nr
Match: XP_023001945.1 (DPH4 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 332.0 bits (850), Expect = 3.1e-87
Identity = 165/180 (91.67%), Postives = 172/180 (95.56%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERY 60
           MSSSSGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQ+MCQKSHPND+ GERY
Sbjct: 40  MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 99

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKG+AMGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 100 FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 159

Query: 121 YFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSFDC 180
           YFFIDSGELEEMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VS DC
Sbjct: 160 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSIDC 219

[Close]

BLAST of Lag0004310 vs. NCBI nr
Match: XP_023523039.1 (DPH4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.5 bits (846), Expect = 9.1e-87
Identity = 163/180 (90.56%), Postives = 171/180 (95.00%), Query Frame = 0


Query: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERY 60
           MSSSSGSIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQ+MCQKSHP+D+ GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPDDVVGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMGAESISLEDMVVEDKGEVVELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKGDAMGAESI LEDM VEDKGE+VELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGDAMGAESIGLEDMAVEDKGEIVELFYQCRCGD 120

Query: 121 YFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSFDC 180
           YFFIDSGELEEMGYP+LR GSKVSLRT DALPAS+VLPCGSCSLKVRLLIDS+S  S DC
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTFDALPASIVLPCGSCSLKVRLLIDSDSCASIDC 180

[Close]

BLAST of Lag0004310 vs. ExPASy Swiss-Prot
Match: Q54CI5 (DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.5e-11
Identity = 48/164 (29.27%), Postives = 78/164 (47.56%), Query Frame = 0


Query: 2   SSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQ------------SMC-- 61
           ++S+    + YY++L +  DA  +EI+ SYR   L +HPDKL             S C  
Sbjct: 6   NNSNKCENKNYYEILKVSIDADIEEIKKSYRKLALLYHPDKLNKEENIEENINNFSNCLV 65

Query: 62  -QKSHPNDIKGERYFKVQKAWEVLGSSTSRAAYDRELQAAKGDAMG-AESISLEDM-VVE 121
              ++ N+   + +  +Q AWE L     R  YD  L   K      ++ I L+DM  +E
Sbjct: 66  NNNNNNNNSNTKDFNDIQIAWETLKDDLLRKQYDSLLLEQKRQKYSVSDEIDLDDMEFIE 125

Query: 122 DKGEVVELFYQCRCGDYFFIDSGELEEMGYPLLRNGSKVSLRTL 149
           +  E V   Y CRCGD++ I   +L E    +  +G  +S++ +
Sbjct: 126 ENSEYV---YPCRCGDHYIITEDQLSEGSDVVCCSGCSLSIKVI 166

[Close]

BLAST of Lag0004310 vs. ExPASy Swiss-Prot
Match: P47138 (Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ3 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 7.9e-09
Identity = 49/154 (31.82%), Postives = 74/154 (48.05%), Query Frame = 0


Query: 11  TYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERYFKVQKAWEVL 70
           T+Y++L +  DA+ DEI+ +YR+ LLN HPDKL     KS  + +      K+Q A+++L
Sbjct: 8   THYEILRIPSDATQDEIKKAYRNRLLNTHPDKL----SKSIHDTVSNVTINKIQDAYKIL 67

Query: 71  GSSTSRAAYDR------ELQAAKGDAMGAESISLEDMVV-EDKGEVVELFYQCR-CGDYF 130
            +  +R  YDR      + Q       G +  SL+D    EDK E +    +C+  G + 
Sbjct: 68  SNIKTRREYDRLILENYKRQGFHNCGDGLDEFSLDDFSFDEDKLEFMMNCPRCQFVGGFH 127

Query: 131 F-----------IDSGELEEMGYPLLRNGSKVSL 146
           F           +D+ E    GY LL   S  SL
Sbjct: 128 FSESLLDECIDNVDAMERSHSGYQLLTQCSACSL 157

[Close]

BLAST of Lag0004310 vs. ExPASy Swiss-Prot
Match: Q6FWM1 (Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DPH4 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.0e-08
Identity = 50/167 (29.94%), Postives = 82/167 (49.10%), Query Frame = 0


Query: 11  TYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERYFKVQKAWEVL 70
           ++Y+VL +  DAS DEI+ +YR  LL  HPDK +++   S      G    ++Q+A+ VL
Sbjct: 10  SHYEVLGVPVDASTDEIKLTYRKMLLTLHPDKSKTVPIDSTIKIRSGVSINQIQEAYRVL 69

Query: 71  GSSTSRAAYDRELQAAK--------GDAMGAESISLEDMVV-EDKGEVVELFYQCRCGDY 130
            +   RAAY+R L  +         GD  G +  +L++    E+K E V    +C+ G  
Sbjct: 70  SNDQLRAAYNRALNDSNKLAGFNNFGD--GLDEFNLDEFEFNEEKLEYVMTCPRCQSGGG 129

Query: 131 FFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRL 169
           F +    LEE     + +G    L   +     V+  C +CSL +++
Sbjct: 130 FVLSEDMLEE----CIEDG----LTESEEQGYQVLTQCTACSLWLKV 166

[Close]

BLAST of Lag0004310 vs. ExPASy Swiss-Prot
Match: B9KH92 (Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.7e-08
Identity = 31/74 (41.89%), Postives = 44/74 (59.46%), Query Frame = 0


Query: 9  GETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERYF-KVQKAW 68
          G  YY++L +  +AS +EI+ SYR  +  +HPDK        +P D K E  F K+ +A+
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPDK--------NPGDKKAEEKFKKISEAY 62

Query: 69 EVLGSSTSRAAYDR 82
          EVL +   RAAYDR
Sbjct: 63 EVLSNPEKRAAYDR 68

[Close]

BLAST of Lag0004310 vs. ExPASy Swiss-Prot
Match: Q5P9E0 (Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.7e-08
Identity = 31/74 (41.89%), Postives = 44/74 (59.46%), Query Frame = 0


Query: 9  GETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQSMCQKSHPNDIKGERYF-KVQKAW 68
          G  YY++L +  +AS +EI+ SYR  +  +HPDK        +P D K E  F K+ +A+
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPDK--------NPGDKKAEEKFKKISEAY 62

Query: 69 EVLGSSTSRAAYDR 82
          EVL +   RAAYDR
Sbjct: 63 EVLSNPEKRAAYDR 68

The following BLAST results are available for this feature:

BLAST of Lag0004310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884890.1	6.3e-88	93.85	DPH4 homolog isoform X2 [Benincasa hispida]	[more]
XP_008444636.1	3.1e-87	91.67	PREDICTED: DPH4 homolog [Cucumis melo]	[more]
XP_023001946.1	3.1e-87	91.67	DPH4 homolog isoform X2 [Cucurbita maxima]	[more]
XP_023001945.1	3.1e-87	91.67	DPH4 homolog isoform X1 [Cucurbita maxima]	[more]
XP_023523039.1	9.1e-87	90.56	DPH4 homolog [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0004310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54CI5	8.5e-11	29.27	DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1	[more]
P47138	7.9e-09	31.82	Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
Q6FWM1	3.0e-08	29.94	Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 1...	[more]
B9KH92	6.7e-08	41.89	Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ...	[more]
Q5P9E0	6.7e-08	41.89	Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=d...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 75..94 score: 25.96 coord: 31..46 score: 34.77 coord: 55..75 score: 22.4 coord: 13..31 score: 38.0
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 10..75 e-value: 4.6E-10 score: 49.4
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 11..80 e-value: 1.0E-14 score: 54.4
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 11..83 score: 14.80416
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 11..71 e-value: 5.53906E-14 score: 61.4073
IPR036671	DPH-type metal-binding domain superfamily	GENE3D	3.10.660.10	DPH Zinc finger	coord: 87..179 e-value: 5.8E-17 score: 63.0
IPR036671	DPH-type metal-binding domain superfamily	SUPERFAMILY	144217	CSL zinc finger	coord: 92..172
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 7..86 e-value: 1.4E-17 score: 65.7
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 10..83
IPR007872	DPH-type metal-binding domain	PFAM	PF05207	zf-CSL	coord: 95..169 e-value: 3.1E-13 score: 49.2
IPR007872	DPH-type metal-binding domain	PROSITE	PS51074	DPH_MB	coord: 92..171 score: 15.106194
None	No IPR available	PANTHER	PTHR21454:SF31	DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4	coord: 1..172
IPR044248	Diphthamide biosynthesis protein 3/4-like	PANTHER	PTHR21454	DPH3 HOMOLOG-RELATED	coord: 1..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004310.1	Lag0004310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding