Lag0004270 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004270 vs. NCBI nr
Match: XP_022962262.1 (glutamate receptor 2.5-like [Cucurbita moschata])

HSP 1 Score: 959.5 bits (2479), Expect = 1.8e-275
Identity = 527/807 (65.30%), Postives = 635/807 (78.69%), Query Frame = 0

Query: 2   LRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNHR--NESMRIGVVFDSGSQIGKQ 61
           LR   C LS  +GLLLL   L SEA TSK  +NCQ N +  N++ RIGVVFDSGSQ+GKQ
Sbjct: 5   LRSCFCGLSFILGLLLLSSSLGSEALTSKSILNCQTNPKPNNKTTRIGVVFDSGSQLGKQ 64

Query: 62  QMIAMKMALRRFHFSS-CVKLELLFHDYSNPNFTS-TSSAVDLITKGGVKTIVGAVRMQG 121
           QM+AMKM L  FH SS C+KLELL HD S+ N TS  SSA+DLITKGGVK +VG+VRMQ 
Sbjct: 65  QMVAMKMGLSHFHLSSPCLKLELLLHD-SHQNLTSPPSSALDLITKGGVKAVVGSVRMQD 124

Query: 122 LIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLF 181
           LIVISD   PVG PIVSTSA+Q++ LKIPSLIQMAN+ THRI+CI SILTHFQWRKVT+F
Sbjct: 125 LIVISDNNFPVGIPIVSTSAEQVEQLKIPSLIQMANHITHRIQCIVSILTHFQWRKVTIF 184

Query: 182 HEIENIDH---PSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRN 241
           +EI NIDH    S++SA+RLFDSLRLA++E+EH L  SSSSNQEILIE+ELKRLKN+QRN
Sbjct: 185 YEISNIDHFPISSAVSANRLFDSLRLADVEVEHRLALSSSSNQEILIEQELKRLKNSQRN 244

Query: 242 GVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIG 301
            VF+VTQL +ELAVL+  KAKK+NMVG NGY WIVS+DVFDL+DSLD SS   KM+GVIG
Sbjct: 245 RVFVVTQLEVELAVLVLEKAKKLNMVG-NGYVWIVSNDVFDLMDSLD-SSFWYKMDGVIG 304

Query: 302 FRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLGEN 361
           F TYF+DTK SFK FETKFKKMY LEY Q E+P +ASI  VRAYDA++A+TRA   +GEN
Sbjct: 305 FGTYFNDTKNSFKSFETKFKKMYRLEYPQEEEPTRASISVVRAYDAARAMTRA---MGEN 364

Query: 362 FSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVE 421
             S+++ ++I +SNFEGLSG V+FKNG LISQSP F+I KV+ QS+K +AFWTP LGF E
Sbjct: 365 LISSEVLEKIGESNFEGLSGRVRFKNGKLISQSPNFKISKVVDQSFKEVAFWTPKLGFFE 424

Query: 422 SFVEVNEAAATKLQP---NMGNVAVRDLSRSLMASSSE----EKRLKFAVPGKGSACQEF 481
            FVEVN+   TKL+P   N+GNVAV DL R  + +SSE    EKRL+FAVP +G ACQE 
Sbjct: 425 RFVEVNK-TTTKLKPKFGNLGNVAVGDLPR--LTTSSENCEGEKRLRFAVPEEG-ACQEI 484

Query: 482 VKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGAV 541
           VKVS H  G Y++GFSI+VFRAVM+  N+  PLSYDL PF+GK +DML AV NKTYDGAV
Sbjct: 485 VKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKTYDGAV 544

Query: 542 GDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFI 601
           G+IGI   RF  VDFTV+YLE +IVMVV EK+ +WKQ W F EAF+V+ WLLIP+MHLFI
Sbjct: 545 GEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSQWKQLWAFTEAFDVSTWLLIPTMHLFI 604

Query: 602 SFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASF 661
           SF +WL+ERQN++ELKGFGN++WFS+SIIFYMHREPVKNGLARLVLGPWLF ILV++ASF
Sbjct: 605 SFSVWLLERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGILVVTASF 664

Query: 662 SASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSID 721
           ++SLTSMMT +W++PSVLDV  LK+M+A VGCN+ SFIC YL  T KFEPSKI ++ S++
Sbjct: 665 TSSLTSMMTVTWYRPSVLDVAKLKEMNAVVGCNARSFICDYLEKTLKFEPSKIKRIASLN 724

Query: 722 DYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSA 781
           +YP AF++ +I+AAFFISPHA     K      +   S  +    +AF KGSPLAA VSA
Sbjct: 725 NYPKAFEDDSIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLAAKVSA 784

Query: 782 SIVELTQTNDISQMEQSLLAPLQLQAA 793
           SIVELT+T ++ Q + + L       A
Sbjct: 785 SIVELTETKEMPQFDPNYLVSFNCPTA 801

BLAST of Lag0004270 vs. NCBI nr
Match: XP_022996842.1 (glutamate receptor 2.5-like [Cucurbita maxima])

HSP 1 Score: 959.1 bits (2478), Expect = 2.3e-275
Identity = 531/818 (64.91%), Postives = 642/818 (78.48%), Query Frame = 0

Query: 1   MLRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNHR--NESMRIGVVFDSGSQIGK 60
           +LR   C LS  +GLLLL   L SEA TSK  +NCQ N +  +++ RIGVVFDSGSQ+GK
Sbjct: 4   VLRICFCRLSFILGLLLLSSSLGSEALTSKSILNCQTNPKPNDKTTRIGVVFDSGSQLGK 63

Query: 61  QQMIAMKMALRRFHF-SSCVKLELLFHDYSNPNFTS-TSSAVDLITKGGVKTIVGAVRMQ 120
           QQ++AMKM L  FH  SSC+KLELL HD S+ N TS  SSA+DLITKGGVK +VG+VRMQ
Sbjct: 64  QQLVAMKMGLSHFHLSSSCLKLELLLHD-SHQNLTSPPSSALDLITKGGVKAVVGSVRMQ 123

Query: 121 GLIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANN--FTHRIKCIASILTHFQWRKV 180
            LIVIS+   PV  PIVSTSA+QL+ LKIPSLIQMAN+   THRI+CIASILTHFQWRKV
Sbjct: 124 DLIVISNNNFPVPIPIVSTSAEQLEQLKIPSLIQMANHITITHRIQCIASILTHFQWRKV 183

Query: 181 TLFHEIENIDHP---SSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNN 240
           T+F++I N DH    S++SA+RLFDSLRLA++E+EH L  SSSSNQEILIE+ELK+L N+
Sbjct: 184 TIFYDISNTDHSPIYSAVSANRLFDSLRLADVEVEHRLALSSSSNQEILIEQELKKLMNS 243

Query: 241 QRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEG 300
           QRN VF+VTQL +ELAVL+  +AKK+NMVG NGY WIVS+DVFD IDSLD SS   KMEG
Sbjct: 244 QRNRVFVVTQLEVELAVLVLEQAKKLNMVG-NGYVWIVSNDVFDAIDSLD-SSFWYKMEG 303

Query: 301 VIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEML 360
           VIGFRTYFDDTK SFK FETKFKKMY LEY Q E+P +ASI  VRAYDA++AITRA   +
Sbjct: 304 VIGFRTYFDDTKNSFKSFETKFKKMYRLEYPQEEEPTRASISVVRAYDAARAITRA---M 363

Query: 361 GENFSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLG 420
           GEN S +++ ++I +SNFEGLSGMV+FKNGMLISQSP F+I KV+GQS+K + FWTP LG
Sbjct: 364 GENLSWSEVLEKIGESNFEGLSGMVRFKNGMLISQSPNFKISKVVGQSFKEVGFWTPKLG 423

Query: 421 FVESFVEVNEAAATKLQP---NMGNVAVRDLSRSLMASSSE----EKRLKFAVPGKGSAC 480
           FVE FVEVN+   TKL+P   N+GNVAV DL R  + +SSE    EKRL+FAVP +G AC
Sbjct: 424 FVERFVEVNK-TTTKLKPKFGNLGNVAVGDLPR--LTTSSENCDGEKRLRFAVPEEG-AC 483

Query: 481 QEFVKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYD 540
           QE VKVS H  G Y++GFSI+VFRAVM+  N+  PLSYDL PF+GK +DML AV NKTYD
Sbjct: 484 QEIVKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKTYD 543

Query: 541 GAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMH 600
           GAVG+IGI   RF  VDFTV+YLE +IVMVV EK+ +WKQ W F +AFEV++WLLIP+MH
Sbjct: 544 GAVGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSRWKQLWAFTDAFEVSMWLLIPTMH 603

Query: 601 LFISFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMS 660
           LFISF +WLIERQN++ELKGFGN++WFS+SIIFYMHREPVKNGLARLVLGPWLF ILV++
Sbjct: 604 LFISFAVWLIERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGILVVT 663

Query: 661 ASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMN 720
           ASF++SLTSMMT SW++PSVLDV  LK+++A VGCN+ SFIC YL  T KFEPSKI +++
Sbjct: 664 ASFTSSLTSMMTVSWYRPSVLDVVKLKEINAVVGCNARSFICDYLKKTLKFEPSKIKRID 723

Query: 721 SIDDYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAAN 780
           S+++YP AF++ TI+AAFFISPHA     K      +   S  +    +AF KGSPLAA 
Sbjct: 724 SLNNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLAAK 783

Query: 781 VSASIVELTQTNDISQMEQSLLAPLQLQAALQLTKQMG 801
           VSASIVELT+T ++ Q + + L       A +    MG
Sbjct: 784 VSASIVELTETKEMPQFDPNYLVSFNCPTAKEPGPFMG 811

BLAST of Lag0004270 vs. NCBI nr
Match: KAG6598368.1 (Glutamate receptor 2.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 954.5 bits (2466), Expect = 5.7e-274
Identity = 526/808 (65.10%), Postives = 634/808 (78.47%), Query Frame = 0

Query: 1   MLRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQ--KNHRNESMRIGVVFDSGSQIGK 60
           +LR   C LS  +G LLL   L SEA TSK  +NCQ   N  N++ RIGVVFDSGSQ+GK
Sbjct: 4   VLRSCFCGLSFILGSLLLSSSLGSEALTSKSILNCQTNPNPNNKTTRIGVVFDSGSQLGK 63

Query: 61  QQMIAMKMALRRFHFSS-CVKLELLFHDYSNPNFTS-TSSAVDLITKGGVKTIVGAVRMQ 120
           QQM+AMKM L  FH SS C+KLELL HD S+ N TS  SSA+DLITKGGVK +VG+VRMQ
Sbjct: 64  QQMVAMKMGLSHFHLSSPCLKLELLLHD-SHQNLTSPPSSALDLITKGGVKAVVGSVRMQ 123

Query: 121 GLIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTL 180
            LIVISD   PVG PIVSTSA+Q++ LKIPSLIQMAN+ THRI+CI SILTHFQWRKVT+
Sbjct: 124 DLIVISDNNFPVGIPIVSTSAEQVEQLKIPSLIQMANHITHRIQCIVSILTHFQWRKVTI 183

Query: 181 FHEIENIDH---PSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQR 240
           F+EI NIDH    S++SA+RLFDSL LA++E+EH L  SSSSNQ+ILIE+ELKRLKN+QR
Sbjct: 184 FYEISNIDHFPISSAVSANRLFDSLWLADVEVEHRLALSSSSNQKILIEQELKRLKNSQR 243

Query: 241 NGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVI 300
           N VF+VTQL +ELAVL+  KAKK+NMVG NGY WIVS+DVFDL+DSLD SS   KM+GVI
Sbjct: 244 NRVFVVTQLEVELAVLVLEKAKKLNMVG-NGYVWIVSNDVFDLMDSLD-SSFWYKMDGVI 303

Query: 301 GFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLGE 360
           GF TYF+DTK SFK FETKFKKMY LEY Q E+P +ASI  VRAYDA++A+TRA   +GE
Sbjct: 304 GFGTYFNDTKNSFKSFETKFKKMYRLEYPQEEEPTRASISVVRAYDAARAMTRA---MGE 363

Query: 361 NFSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFV 420
           N  S+++ ++I +SNFEGLSG V+FKNG LISQSP F+I KV+ QS+K +AFWTP LGFV
Sbjct: 364 NLISSEVLEKIGESNFEGLSGRVRFKNGKLISQSPNFKISKVVDQSFKEVAFWTPKLGFV 423

Query: 421 ESFVEVNEAAATKLQP---NMGNVAVRDLSRSLMASSSE----EKRLKFAVPGKGSACQE 480
           E FVEVN+   TKL+P   N+GNVAV DL R  + +SSE    EKRL+FAVP +G ACQE
Sbjct: 424 ERFVEVNK-TTTKLKPKFGNLGNVAVGDLPR--LTTSSENCEGEKRLRFAVPEEG-ACQE 483

Query: 481 FVKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGA 540
            VKVS H  G Y++GFSI+VFRAVM+  N+  PLSYDL PF+GK +DML AV NKTYDGA
Sbjct: 484 IVKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKTYDGA 543

Query: 541 VGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLF 600
           VG+IGI   RF  VDFTV+YLE +IVMVV EK+ +WKQ W F EAF+V+ WLLIP+MHLF
Sbjct: 544 VGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSQWKQLWAFTEAFDVSTWLLIPTMHLF 603

Query: 601 ISFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSAS 660
           ISF +WL+ERQN++ELKGFGN++WFS+SIIFYMHREPVKNGLARLVLGPWLF ILV++AS
Sbjct: 604 ISFSVWLLERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGILVVTAS 663

Query: 661 FSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSI 720
           F++SLTSMMT SW++PSVLDV  LK+M+A VGCN+ SFIC YL  T KFEPSKI ++ S+
Sbjct: 664 FTSSLTSMMTVSWYRPSVLDVAKLKEMNAVVGCNARSFICDYLEKTLKFEPSKIKRIISL 723

Query: 721 DDYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVS 780
           ++YP AF++ +I+AAFFISPHA     K      +   S  +    +AF KGSPLAA VS
Sbjct: 724 NNYPKAFEDDSIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLAAKVS 783

Query: 781 ASIVELTQTNDISQMEQSLLAPLQLQAA 793
           ASIVELT+T ++ Q + + L       A
Sbjct: 784 ASIVELTETKEMPQFDPNYLVSFNCPTA 801

BLAST of Lag0004270 vs. NCBI nr
Match: XP_023546335.1 (glutamate receptor 2.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 945.7 bits (2443), Expect = 2.7e-271
Identity = 518/807 (64.19%), Postives = 630/807 (78.07%), Query Frame = 0

Query: 2   LRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNHR--NESMRIGVVFDSGSQIGKQ 61
           LR   C LS  +GLLLL   L SEA TSK  +NCQ N +  N++ RIGVVFDSGSQ+GKQ
Sbjct: 5   LRSCFCGLSFILGLLLLSSSLGSEALTSKSILNCQTNPKPNNKTTRIGVVFDSGSQLGKQ 64

Query: 62  QMIAMKMALRRFHF-SSCVKLELLFHDYSNPNFTS-TSSAVDLITKGGVKTIVGAVRMQG 121
           QM+AMKM L  FH  SSC+KLELL HD S+ N TS  SSA+DLITKGGVK +VG+VRMQ 
Sbjct: 65  QMVAMKMGLSHFHLSSSCLKLELLLHD-SHQNLTSPPSSALDLITKGGVKAVVGSVRMQD 124

Query: 122 LIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLF 181
           LI+ISD   PVG PIVSTSA+QL+ LKIPSLIQMAN+  HRI+CIASILTHFQWRKVT+F
Sbjct: 125 LIMISDNNFPVGVPIVSTSAEQLEQLKIPSLIQMANHINHRIQCIASILTHFQWRKVTIF 184

Query: 182 HEIENIDHP---SSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRN 241
           +EI + DH     ++SA+RLFDSLRLA++E+EH L  SSSSNQEILIE EL++L N+QRN
Sbjct: 185 YEISHTDHSPIYCAVSANRLFDSLRLADVEVEHRLALSSSSNQEILIEHELQKLMNSQRN 244

Query: 242 GVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIG 301
            VF+VTQL +ELAV++  +AKK+NMVG NGY WIVS+DVFDL+DSLD SS   KM+GVIG
Sbjct: 245 RVFVVTQLEVELAVIVLERAKKLNMVG-NGYVWIVSNDVFDLMDSLD-SSFWYKMDGVIG 304

Query: 302 FRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLGEN 361
           F TYF+DTK SFK FETKFKKMY LEY Q E P +ASI  VRAYDA++A+TRA   +GEN
Sbjct: 305 FGTYFNDTKNSFKSFETKFKKMYRLEYPQEEDPTRASISVVRAYDAARAMTRA---MGEN 364

Query: 362 FSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVE 421
             S+++ ++I +SNFEGLSG V+FKNGML+SQSP F+I KV  QS+K +AFWTP LG VE
Sbjct: 365 LISSEVLEKIGESNFEGLSGTVRFKNGMLVSQSPNFKISKVADQSFKEVAFWTPKLGIVE 424

Query: 422 SFVEVNEAAATKLQP---NMGNVAVRDLSRSLMASSSE----EKRLKFAVPGKGSACQEF 481
            FVEVN+   TKL+P   N+GNV V DL+R  + +SSE    EKRL+FAVP +G ACQE 
Sbjct: 425 RFVEVNK-TTTKLKPKFGNLGNVGVGDLAR--LTTSSENFQGEKRLRFAVPEEG-ACQEI 484

Query: 482 VKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGAV 541
           VKVS H  G Y++GFSI+VFRAVM+  N+  PLSYDL PF+GK +DML AV NKTYDGAV
Sbjct: 485 VKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKTYDGAV 544

Query: 542 GDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFI 601
           G+IGI   RF  VDFTV+YLE +IVMVV EK+ +WKQ W F EAF+V+ WLLIP+MHLFI
Sbjct: 545 GEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSQWKQLWAFTEAFDVSTWLLIPTMHLFI 604

Query: 602 SFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASF 661
           SF +WL+ERQN++ELKGFGN++WFS+SIIFYMHREPVKNGLARLVLGPWLF I+V++ASF
Sbjct: 605 SFSVWLLERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGIMVVTASF 664

Query: 662 SASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSID 721
           ++SLTSMMT SW++PSVLDV  LK+M+A VGCN+ SFIC YL  T KFEPSKI ++ S++
Sbjct: 665 TSSLTSMMTVSWYRPSVLDVAKLKEMNAVVGCNARSFICDYLEKTLKFEPSKIKRIASLN 724

Query: 722 DYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSA 781
           +YP AF++ +I+AAFFISPHA     K      +   S  +    +AF KGSPLAA VSA
Sbjct: 725 NYPKAFEDDSIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLAAKVSA 784

Query: 782 SIVELTQTNDISQMEQSLLAPLQLQAA 793
           SIVELT+T ++ Q + + L       A
Sbjct: 785 SIVELTETKEMPQFDPNYLVSFNCPTA 801

BLAST of Lag0004270 vs. NCBI nr
Match: XP_038884567.1 (glutamate receptor 2.5-like [Benincasa hispida])

HSP 1 Score: 939.5 bits (2427), Expect = 1.9e-269
Identity = 537/811 (66.21%), Postives = 638/811 (78.67%), Query Frame = 0

Query: 1   MLRYSSCCLSCFVGLLLLLLLLSSEAHTS-KREVNCQKNHRNESMRIGVVFDSGSQIGKQ 60
           +L YS   L  F+G    LLLL SEA+TS K E+NCQKN  N++ RIGV FDSGSQIGKQ
Sbjct: 5   VLLYSFFSLFFFLG----LLLLGSEAYTSIKLELNCQKNLPNKTTRIGVFFDSGSQIGKQ 64

Query: 61  QMIAMKMALRRFHFSSCVKLELLFHDYSNPNFTSTSSA---VDLITKGGVKTIVGAVRMQ 120
           Q++AMKMALRRFHFSSC KLELL HD S+ N+ ++SSA   +DLITKG VK +VG+VR Q
Sbjct: 65  QIVAMKMALRRFHFSSCAKLELLLHD-SHANYVNSSSASSVLDLITKGRVKAVVGSVRKQ 124

Query: 121 GLIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMA--NNFTHRIKCIASILTHFQ-WRK 180
            LIVISD KIPV  PI+STSA+QLQPLKIPSLIQM   NN TH I CIASILT+F+   K
Sbjct: 125 DLIVISDHKIPVEIPIISTSAEQLQPLKIPSLIQMVDNNNITHPIHCIASILTYFECLPK 184

Query: 181 VTLFHEIENIDHPSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQR 240
           VT+F++  N       SA  LFDS  LA IE+EHHL  S +SNQEILIE+ELK++ ++QR
Sbjct: 185 VTIFYQFTN------TSAHCLFDSFLLAGIEVEHHLALSLASNQEILIEEELKKIMSSQR 244

Query: 241 NGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLD-SSSLLNKMEGV 300
           N VFIVTQLSLEL  LL TKAKKMNMVG NGYTWIVS ++FDLI SLD SSSLLNKMEGV
Sbjct: 245 NRVFIVTQLSLELVDLLLTKAKKMNMVG-NGYTWIVSHEIFDLISSLDSSSSLLNKMEGV 304

Query: 301 IGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLG 360
           IGF+TYF+DTK+SFK FETKFKK+Y LEY Q E+P KASIL +RAYDA+ AITRA+E LG
Sbjct: 305 IGFQTYFNDTKRSFKIFETKFKKIYKLEYPQEEEPTKASILAIRAYDAALAITRAMEKLG 364

Query: 361 -ENF--SSTQLTKQILKSNFEGLSGMVKF--KNGMLISQSPKFQIIKVMGQSYKGIAFWT 420
            EN   SS QL K+IL+SNFEG+ GMV+F  KNGMLISQSPKF+IIKV+ Q+YK + FWT
Sbjct: 365 TENLRSSSEQLMKKILESNFEGVGGMVRFSKKNGMLISQSPKFKIIKVVDQTYKEVGFWT 424

Query: 421 PNLGFVES-FVEVNEAAATKLQPNMGN----VAVRDLSRSLMASSS------EEKRLKFA 480
           P LGFVE   VE+N+   T L+ NM N    V VRDLSR L AS S      EEKRLKFA
Sbjct: 425 PILGFVERVIVEINK-PTTNLKSNMRNLRNYVGVRDLSR-LKASPSEKFDHHEEKRLKFA 484

Query: 481 VPGKGSACQEFVKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLH 540
           VP +G AC+EFVKVS+H  G YI+G+SIDVFRAVMN  NM  PLSYDL PF+G  D+M+ 
Sbjct: 485 VPEEG-ACKEFVKVSHHLYGNYITGYSIDVFRAVMNNMNMSHPLSYDLVPFKGTYDEMIE 544

Query: 541 AVYNKTYDGAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTV 600
           AV NKTY GAVGDIGI A R+++VDFTV+YLE +IVMVV EK EKWK+ W FMEAF+ T+
Sbjct: 545 AVSNKTYIGAVGDIGILARRYKYVDFTVSYLETEIVMVVKEKHEKWKKIWAFMEAFQFTM 604

Query: 601 WLLIPSMHLFISFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPW 660
           WLLIP+MHLFISFVIW IERQNN+ELKG GN++WFS+SIIFYMHREPVKNGLARLVLGPW
Sbjct: 605 WLLIPTMHLFISFVIWFIERQNNEELKGLGNMLWFSVSIIFYMHREPVKNGLARLVLGPW 664

Query: 661 LFAILVMSASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFE 720
           LFAILV++ASF+ASLTSMMT SW +P V DV+TLK+M ATVGCN+ SFIC+YL++T +F+
Sbjct: 665 LFAILVVTASFTASLTSMMTISWSRPLVPDVDTLKQMGATVGCNTNSFICNYLYETLQFD 724

Query: 721 PSKIVQMNSIDDYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFP 780
           P+KI ++NS+++YP AF+N +I+AAFFISPHAK    K  +   R   S  +    +A  
Sbjct: 725 PTKIKKINSLNEYPNAFENGSIKAAFFISPHAKVFLAKYCKGYTRGVSSFKLTGIGFALA 784

Query: 781 KGSPLAANVSASIVELTQTNDISQMEQSLLA 786
           KGS L + VSASIVELT+T +I Q E +++A
Sbjct: 785 KGSGLTSLVSASIVELTETKEIPQFESNVIA 800

BLAST of Lag0004270 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.8e-68
Identity = 215/781 (27.53%), Postives = 380/781 (48.66%), Query Frame = 0

Query: 39  HRNESMRIGVVFDSGSQIGKQQMIAMKMALRRF---HFSSCVKLELLFHDYSNPNFTSTS 98
           ++   +++GVV D  +   K  + ++KMA+  F   H +   +L L   D       +++
Sbjct: 25  NQTSEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPNYLTRLTLHVRDSMEDTVQASA 84

Query: 99  SAVDLITKGGVKTIVGAVR-MQGLIVIS-DKKIPVGTPIVSTSAKQLQPLKIPSLIQMAN 158
           +A+DLI    V  I+G +  MQ   +I    K  V T   S ++  L  +K P  ++   
Sbjct: 85  AALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTITFSATSPLLTSIKSPYFVRATI 144

Query: 159 NFTHRIKCIASILTHFQWRKVTLFHEIENIDHPSSISASRLFDSLRLANIEIEHHLTFSS 218
           + + +++ IASI   F+WR+V   + ++N      +    LFD+L+  ++E++  +    
Sbjct: 145 DDSSQVRAIASIFKFFRWRRVVAIY-VDNEFGEGFMPF--LFDALQ--DVEVKRSVIPPE 204

Query: 219 SSNQEILIEKELKRLKNNQRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDV 278
           + + E  I+KEL++L   Q   VF+V  +   LA+ +F  A+ + M+   GY W++++ +
Sbjct: 205 AIDDE--IQKELRKLMERQAR-VFVV-HMESSLALRVFQIARDIGMM-EEGYVWLMTNGM 264

Query: 279 FDLIDSLDSSSLLNKMEGVIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASIL 338
             ++  +++   LN +EGV+G R++   +K+    F  ++K+ +  E +        ++ 
Sbjct: 265 THMMRHINNGRSLNTIEGVLGVRSHVPKSKE-LGDFRLRWKRTF--EKENPSMRDDLNVF 324

Query: 339 EVRAYDASQAITRAVEM-----------------------LGENFSSTQLTKQILKSNFE 398
            + AYD+  A+ +AVE                        +G +     L K   +  F 
Sbjct: 325 ALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSEVRFN 384

Query: 399 GLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVESFVEVNEAAATKLQPN 458
           GL+G  K  +G L  QSPKF+II  +G   + I FWTP  G +++     +     + P 
Sbjct: 385 GLAGEFKLIDGQL--QSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLGPVIWPG 444

Query: 459 MGNVAVRDLSRSLMASSSEEKRLKFAVPGKGSACQEFVKVSNH--SNGIYISGFSIDVFR 518
              +  +             K+L+  VP K     +FVKV+ +  +N    +G++I++F 
Sbjct: 445 KSKIVPK-------GWEIPGKKLRVGVPMK-KGFFDFVKVTINPITNKKTPTGYAIEIFE 504

Query: 519 AVMNNMPGPLSYDLFPFEGKN--DDMLHAVYNKTYDGAVGDIGISAYRFQHVDFTVAYLE 578
           A +  +P  +  +   FE  N  +++++ VY+KT+D  VGDI I+A R  + DFT+ + E
Sbjct: 505 AALKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTE 564

Query: 579 ADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWLIERQNNDELKG---- 638
           + + M+V  +  + K  WVF+E + + +W+      +FI FV+WL E + N + +G    
Sbjct: 565 SGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQY 624

Query: 639 -FGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLTSMMTKSWFQPS 698
             G  +WFS S + + HRE V + LAR V+  W F +LV++ S++ASLTS +T    QP+
Sbjct: 625 QIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPT 684

Query: 699 VLDVETLKKMDATVGCNSESFICSYLHDT--HKFEPSKIVQMNSIDDYPAAFKNRTIEAA 758
           V +V  L K    VG    +F+   L     H+ +          DD  +  K++ I AA
Sbjct: 685 VTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFDSAKDADDLLSKGKSKGIAAA 744

Query: 759 FFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSASIVELTQTNDISQM 781
           F    + K    +          +   G   +AFPK SPL    S +I+ LTQ N   Q+
Sbjct: 745 FDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQI 782

BLAST of Lag0004270 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 1.1e-65
Identity = 227/826 (27.48%), Postives = 407/826 (49.27%), Query Frame = 0

Query: 9   LSCFVGLLLLLLLLSSEAHTSKREVNCQKNHRNESMRIGVVFDSGSQIGKQQMIAMKMAL 68
           +S F+ L LL+ L+       K +    +      +++G+V  S   +    + A+ M+L
Sbjct: 8   VSRFLSLWLLIFLVFLVLSLGKSQKEALQ------VKVGIVLGSNVTLADLSLRAINMSL 67

Query: 69  RRF---HFSSCVKLELLFHDYSNPNFTSTSSAVDLITKGGVKTIVG-AVRMQGLIVI--- 128
             F   H     ++ L   D       + +SA+ LI K  V  I+G    MQ   +I   
Sbjct: 68  SEFYNTHNGFKTRIVLNVRDSKQTVVGAAASALYLIKKREVVAIIGPGTSMQAPFLINLG 127

Query: 129 SDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLFHEIE 188
           +  K+P+ +   S ++  L  L+ P  I+  ++ + +++ I++I+  F+WR+V   + ++
Sbjct: 128 NQSKVPIIS--FSATSPLLDSLRSPYFIRATHDDSSQVQAISAIIESFRWREVVPIY-VD 187

Query: 189 NIDHPSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQ 248
           N +    I    L D+ +  N+ I +    S   + +  I+KEL +L       VFIV  
Sbjct: 188 N-EFGEGI-LPNLVDAFQEINVRIRYRSAISLHYSDD-QIKKELYKLM-TMPTRVFIVHM 247

Query: 249 LSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDD 308
           L  +L   LF+ AK+++M+ + GY WIV++ + DL+  +  SSL+N M GV+G +TYF  
Sbjct: 248 LP-DLGSRLFSIAKEIDML-SKGYVWIVTNGIADLMSIMGESSLVN-MHGVLGVKTYFAK 307

Query: 309 TKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVE------------ 368
           +K+     E +++K +  E   N            AYDA+ A+  +VE            
Sbjct: 308 SKE-LLHLEARWQKRFGGEELNN--------FACWAYDAATALAMSVEEIRHVNMSFNTT 367

Query: 369 --------------MLGENFSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKV 428
                          LG   S  +L   +   +F+G++G  + KNG L  ++  F+II +
Sbjct: 368 KEDTSRDDIGTDLDELGVALSGPKLLDALSTVSFKGVAGRFQLKNGKL--EATTFKIINI 427

Query: 429 MGQSYKGIAFWTPNLGFVESF-VEVNEAAATKLQPNMGNVAVRDLSRSLMASSSEEKRLK 488
                + + FW   +G V+S  V+    ++ +L+P +       + +      +  K+L+
Sbjct: 428 EESGERTVGFWKSKVGLVKSLRVDKVSHSSRRLRPIIWPGDTIFVPKG-WEFPTNAKKLR 487

Query: 489 FAVPGKGSACQEFVKVS--NHSNGIYISGFSIDVFRAVMNNMPGPLSYDLFPFE------ 548
            AVP K      FV+V+   ++N   ++GF IDVF  VM+ MP  +SY+  PF+      
Sbjct: 488 IAVP-KKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKP 547

Query: 549 -GKNDDMLHAVYNKTYDGAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWV 608
            G  D+M++ V+   +DGAVGD  I A R  +VDF + Y E  IV +V  K  K K  WV
Sbjct: 548 RGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWV 607

Query: 609 FMEAFEVTVWLLIPSMHLFISFVIWLIERQNNDELK------GFGNLVWFSISIIFYMHR 668
           F++     +WL+  +  L+I  ++W+ E Q ++E +         ++ +FS S +F+ HR
Sbjct: 608 FLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHR 667

Query: 669 EPVKNGLARLVLGPWLFAILVMSASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNS 728
            P ++   R+++  W F +L+++ S++A+LTSM+T    +P+V  ++ L+K    +G  +
Sbjct: 668 RPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQT 727

Query: 729 ESFICSYLHDTHKFEPSKIVQMNSIDDYPAAF----KNRTIEAAFFISPHAK-FSSKKIA 781
            SF    L    +F+ S++   NS ++    F     N  I+AAF    + K F +K  +
Sbjct: 728 GSFTFERLKQM-RFDESRLKTYNSPEEMRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCS 787

BLAST of Lag0004270 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 252.3 bits (643), Expect = 1.8e-65
Identity = 212/787 (26.94%), Postives = 377/787 (47.90%), Query Frame = 0

Query: 35  CQKNHRNESMRIGVVFDSGSQIGKQQMIAMKMALR---RFHFSSCVKLELLFHDYSNPNF 94
           C   ++   +++GVV D  +   K  + ++ ++L    ++H     +L +   D      
Sbjct: 30  CLGQNQTTEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYHSDYTTRLAIHIRDSMEDVV 89

Query: 95  TSTSSAVDLITKGGVKTIVG---AVRMQGLIVISDKKIPVGTPIVSTSAKQLQPLKIPSL 154
            ++S+A+DLI    V  I+G   +++ + +I ++DK   V T   S +   L  +  P  
Sbjct: 90  QASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKS-QVPTITFSATCPLLTSINSPYF 149

Query: 155 IQMANNFTHRIKCIASILTHFQWRKVTLFHEIENIDHPSSISASRLFDSLRLANIEIEHH 214
           ++   + + ++K IA+I+  F WR V   + ++N      +    L D+L+     + + 
Sbjct: 150 VRATLDDSSQVKAIAAIVKSFGWRNVVAIY-VDNEFGEGILPL--LTDALQDVQAFVVNR 209

Query: 215 LTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWI 274
                 +N + ++ KEL +L   Q   VF+V  +   L    F KA+++ M+   GY W+
Sbjct: 210 CLIPQEANDDQIL-KELYKLMTMQTR-VFVV-HMPPTLGFRFFQKAREIGMM-EEGYVWL 269

Query: 275 VSDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPR 334
           ++D V +L+ S +  S L  M+GV+G R++   +KK  K F  +++KM+  +    E   
Sbjct: 270 LTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKK-LKNFRLRWEKMFPKKGNDEEM-- 329

Query: 335 KASILEVRAYDASQAITRAVE-----------------------MLGENFSSTQLTKQIL 394
             +I  +RAYD+  A+  AVE                        LG +     L K + 
Sbjct: 330 --NIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALS 389

Query: 395 KSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVES-FVEVNEAAA 454
              F GL+G  +  NG L  +S  F +I ++G   + I  W P+ G V +          
Sbjct: 390 NVRFNGLAGEFELINGQL--ESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSVLG 449

Query: 455 TKLQPNMGNVAVRDLSRSLMASSSEEKRLKFAVPGKGSACQEFV--KVSNHSNGIYISGF 514
            +L P +     +D+ +     ++  K L+  +P K     EFV  K+   SN +  +G+
Sbjct: 450 ERLGPVIWPGKSKDVPKGWQIPTN-GKMLRVGIPVK-KGFLEFVDAKIDPISNAMTPTGY 509

Query: 515 SIDVFRAVMNNMPGPL--SYDLFPFEGKN-DDMLHAVYNKTYDGAVGDIGISAYRFQHVD 574
            I++F AV+  +P  +   Y  F    +N D+M++ VY   YD  VGD+ I A R  +VD
Sbjct: 510 CIEIFEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVD 569

Query: 575 FTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWLIERQNNDE 634
           FT+ Y E+ + M+V  K    K  WVF+  + + +W+      +FI F++W++E + N +
Sbjct: 570 FTLPYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTD 629

Query: 635 LKG-----FGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLTSMMT 694
            +G      G   WF+ S + + HRE V + LAR V+  W F +LV+  S++A+LTS  T
Sbjct: 630 FRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFT 689

Query: 695 KSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDDYPAAFKNR 754
               QP+V + + L K +  +G    +F+   L  +  F+ S++    S  +    F N 
Sbjct: 690 VKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELL-KSQGFDESQLKPFGSAVECDELFSNG 749

Query: 755 TIEAAFFISPHAK-FSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSASIVELTQT 781
           TI A+F    + K   S+  ++ T  +    + G   + FPK SPL  +VS +I+ +TQ 
Sbjct: 750 TITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFG-FVFPKKSPLTDDVSRAILNVTQG 795

BLAST of Lag0004270 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 1.7e-63
Identity = 225/818 (27.51%), Postives = 392/818 (47.92%), Query Frame = 0

Query: 9   LSCFVGLLLLLLLLSSEAHTSKREVNCQKNHRNESMRIGVVFDSGSQIGKQQMIAMKMAL 68
           LS FV L LLL            EV   +N  +E +++GVV D  +   K  + ++ +AL
Sbjct: 11  LSYFVCLFLLL------------EVGLGQNQISE-IKVGVVLDLNTTFSKICLTSINLAL 70

Query: 69  RRF---HFSSCVKLELLFHDYSNPNFTSTSSAVDLITKGGVKTIVGAV-RMQGLIVIS-D 128
             F   H +   +L L   D       ++++A+DLI    V  I+G +  MQ   +I   
Sbjct: 71  SDFYKDHPNYRTRLALHVRDSMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLA 130

Query: 129 KKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLFHEIENI 188
            K  V T   S ++  L  +K    ++   + ++++K IA+I   F WR V   +    +
Sbjct: 131 NKTQVPTISFSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNEL 190

Query: 189 DHPSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQLS 248
                     LFD+L+  +++++  +  S +++ +IL  KEL +L   Q   VF+V  ++
Sbjct: 191 GEG---IMPYLFDALQ--DVQVDRSVIPSEANDDQIL--KELYKLMTRQTR-VFVV-HMA 250

Query: 249 LELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDDTK 308
             LA  +F KA ++ M+   GY W++++ +  ++  +     LN ++GV+G R++   + 
Sbjct: 251 SRLASRIFEKATEIGMM-EEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKS- 310

Query: 309 KSFKRFETKFKKMYSLEYQQNEQPR-KASILEVRAYDASQAITRAVEMLGENFSS----- 368
           K  + F  ++K+ +    ++N   R   SI  + AYD++ A+  AVE    N SS     
Sbjct: 311 KGLEDFRLRWKRNFK---KENPWLRDDLSIFGLWAYDSTTALAMAVEK--TNISSFPYNN 370

Query: 369 --------------------TQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMG 428
                                 L + + +  F GL+G     +  L  +SPKF+II  +G
Sbjct: 371 ASGSSNNMTDLGTLHVSRYGPSLLEALSEIRFNGLAGRFNLIDRQL--ESPKFEIINFVG 430

Query: 429 QSYKGIAFWTPNLGFVESFVEVNEAAATKLQPNMGNVAVRDLSRSLMASSSE----EKRL 488
              + + FWTP+ G     V VN    T          +     +++    E     K++
Sbjct: 431 NEERIVGFWTPSNG----LVNVNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNGKKI 490

Query: 489 KFAVPGKGSACQEFVKVSNH--SNGIYISGFSIDVFRAVMNNMPGPLSYDLFPFEGKN-- 548
           K  VP K      FV+V     +N     G++ID+F A +  +P  +    + FE  +  
Sbjct: 491 KVGVPVK-KGFFNFVEVITDPITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPDDD 550

Query: 549 -DDMLHAVYNKTYDGAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFME 608
            DD+++ V N T D  VGD+ I+AYR  + DFT+ Y E+ + M+V  +  + K  WVF++
Sbjct: 551 YDDLVYKVDNGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLK 610

Query: 609 AFEVTVWLLIPSMHLFISFVIWLIERQNNDELKG-----FGNLVWFSISIIFYMHREPVK 668
            + + +W+      + I FV+WL E + N + +G      G   WFS S + + HRE V 
Sbjct: 611 PWGLDLWVTTACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVV 670

Query: 669 NGLARLVLGPWLFAILVMSASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFI 728
           + LAR V+  W F +LV++ S++A+LTS +T   FQP+ ++V+ L K    VG    +F+
Sbjct: 671 SNLARFVVVVWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFV 730

Query: 729 CSYLHDTHKFEPSKIVQMNSIDDYPAAFKNRTIEAAFFISPHAK-FSSKKIAEATPRQSL 781
             +L     F  SK+    S ++  A   N +I AAF    + +   S+  ++    +  
Sbjct: 731 KDFL-IKEGFNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLRAILSQYCSKYAIVEPT 790

BLAST of Lag0004270 vs. ExPASy Swiss-Prot
Match: Q9LFN8 (Glutamate receptor 2.6 OS=Arabidopsis thaliana OX=3702 GN=GLR2.6 PE=2 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 2.5e-62
Identity = 214/799 (26.78%), Postives = 379/799 (47.43%), Query Frame = 0

Query: 44  MRIGVVFDSGSQIGKQQMIAMKMALRRF---HFSSCVKLELLFHDYSNPNFTSTSSAVDL 103
           +++G+V D+ + +    + A+ M+L  F   H     ++ L   D       + +SA+ L
Sbjct: 36  VQVGIVLDTNATLAALSLRAINMSLSEFYNTHNGFKTRIVLNIRDSKRTVVGAAASALYL 95

Query: 104 ITKGGVKTIVGAVRMQGLIVISDKKIPVGTPIVSTSAKQ--LQPLKIPSLIQMANNFTHR 163
           I K  V  I+G         + +       PI+S SA    L  L+ P  I+  ++ + +
Sbjct: 96  IKKREVVAIIGPGNSMQAPFLINLGNQSQVPIISFSASSPVLDSLRSPYFIRATHDDSSQ 155

Query: 164 IKCIASILTHFQWRKVTLFHEIENIDHPSSISASRLFDSLRLANIEIEHHLTFSSSSNQE 223
           +  I++I+  F+WR+V   +               L D+ +  N+ I +    S  S  +
Sbjct: 156 VHAISAIIESFRWREVVPIYADNEFGEG---ILPYLVDAFQEINVRIRYRSAISVHSTDD 215

Query: 224 ILIEKELKRLKNNQRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLID 283
            L++KEL +L       VFIV  L  +L   LF+ AK++ M+   GY WIV++ + D + 
Sbjct: 216 -LVKKELYKLM-TMPTRVFIVHMLP-DLGSRLFSIAKEIGMM-TKGYVWIVTNGIADQMS 275

Query: 284 SLDSSSLLNKMEGVIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAY 343
            +  SSL N M GV+G +TYF  +K+     ET+++K +  E   N         E   Y
Sbjct: 276 VMGESSLEN-MHGVLGVKTYFSRSKE-LMYLETRWRKRFGGEELNN--------FECWGY 335

Query: 344 DASQAITRAVEMLGEN----FSST-----------------------QLTKQILKSNFEG 403
           D + A+  ++E +  N    FS T                       +L + +   +F+G
Sbjct: 336 DTATALAMSIEEISSNVNMSFSQTKRNTSRDDTGTDLDDLSFALSGPKLLQALATVSFKG 395

Query: 404 LSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVESFVEVNEA------AAT 463
           ++G  + KNG L  ++  F+I+ +     + + FW   +G V+S + VN+       ++ 
Sbjct: 396 VAGRFQLKNGKL--EATTFKIVNIEESGERTVGFWKSKVGLVKS-LRVNQTGIKISHSSH 455

Query: 464 KLQPNMGNVAVRDLSRSLMASSSEEKRLKFAVPGKGSACQEFVKVSN--HSNGIYISGFS 523
           +L+P +       + +      +  K+L+ AVP K      FV+V+   ++N   I+GF 
Sbjct: 456 RLRPIIWPGDTIFVPKG-WEFPTNAKKLRIAVP-KKDGFNNFVEVTKDANTNAPTITGFC 515

Query: 524 IDVFRAVMNNMPGPLSYDLFPFE-------GKNDDMLHAVYNKTYDGAVGDIGISAYRFQ 583
           IDVF   M  MP  + Y+  PFE       G  D+M++ V+   +DGAVGD  I A R  
Sbjct: 516 IDVFDTAMRQMPYAVPYEYIPFETPDGKPRGSYDEMVYHVFLGEFDGAVGDTTILANRST 575

Query: 584 HVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWLIERQN 643
           +VDF + Y E  IV+VV  K E+ K  WVF++     +W L  +  L+I  ++W+ E Q 
Sbjct: 576 YVDFALPYSETGIVVVVPVKDEREKGKWVFLKPLTRELWFLTAASFLYIGIMVWIFEYQA 635

Query: 644 NDE------LKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLT 703
           + +      +    N+ +FS S +F+ H  P ++   R+++  W F +L+++ S++A+LT
Sbjct: 636 SGDFRKQSIINKISNVFYFSFSTLFFAHMRPSESIFTRVLVVVWCFVLLILTQSYTATLT 695

Query: 704 SMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDDYPAA 763
           SM+T    +P+V  ++ L+     +G  + SF    L     ++ S++   ++  +    
Sbjct: 696 SMLTVQELRPTVRHMDDLRNSGVNIGYQTGSFTFERLKQM-GYKESRLKTYDTPQEMHEL 755

Query: 764 F----KNRTIEAAFFISPHAK-FSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSA 785
           F     N  I+AAF    + K F +K  ++ T  +    + G   +AFP GSPL  ++S 
Sbjct: 756 FLKKSSNGGIDAAFDEVAYVKLFMAKYCSKYTIIEPTFKADGFG-FAFPLGSPLVPDLSR 810

BLAST of Lag0004270 vs. ExPASy TrEMBL
Match: A0A6J1HC86 (glutamate receptor 2.5-like OS=Cucurbita moschata OX=3662 GN=LOC111462764 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 8.6e-276
Identity = 527/807 (65.30%), Postives = 635/807 (78.69%), Query Frame = 0

Query: 2   LRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNHR--NESMRIGVVFDSGSQIGKQ 61
           LR   C LS  +GLLLL   L SEA TSK  +NCQ N +  N++ RIGVVFDSGSQ+GKQ
Sbjct: 5   LRSCFCGLSFILGLLLLSSSLGSEALTSKSILNCQTNPKPNNKTTRIGVVFDSGSQLGKQ 64

Query: 62  QMIAMKMALRRFHFSS-CVKLELLFHDYSNPNFTS-TSSAVDLITKGGVKTIVGAVRMQG 121
           QM+AMKM L  FH SS C+KLELL HD S+ N TS  SSA+DLITKGGVK +VG+VRMQ 
Sbjct: 65  QMVAMKMGLSHFHLSSPCLKLELLLHD-SHQNLTSPPSSALDLITKGGVKAVVGSVRMQD 124

Query: 122 LIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLF 181
           LIVISD   PVG PIVSTSA+Q++ LKIPSLIQMAN+ THRI+CI SILTHFQWRKVT+F
Sbjct: 125 LIVISDNNFPVGIPIVSTSAEQVEQLKIPSLIQMANHITHRIQCIVSILTHFQWRKVTIF 184

Query: 182 HEIENIDH---PSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRN 241
           +EI NIDH    S++SA+RLFDSLRLA++E+EH L  SSSSNQEILIE+ELKRLKN+QRN
Sbjct: 185 YEISNIDHFPISSAVSANRLFDSLRLADVEVEHRLALSSSSNQEILIEQELKRLKNSQRN 244

Query: 242 GVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIG 301
            VF+VTQL +ELAVL+  KAKK+NMVG NGY WIVS+DVFDL+DSLD SS   KM+GVIG
Sbjct: 245 RVFVVTQLEVELAVLVLEKAKKLNMVG-NGYVWIVSNDVFDLMDSLD-SSFWYKMDGVIG 304

Query: 302 FRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLGEN 361
           F TYF+DTK SFK FETKFKKMY LEY Q E+P +ASI  VRAYDA++A+TRA   +GEN
Sbjct: 305 FGTYFNDTKNSFKSFETKFKKMYRLEYPQEEEPTRASISVVRAYDAARAMTRA---MGEN 364

Query: 362 FSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVE 421
             S+++ ++I +SNFEGLSG V+FKNG LISQSP F+I KV+ QS+K +AFWTP LGF E
Sbjct: 365 LISSEVLEKIGESNFEGLSGRVRFKNGKLISQSPNFKISKVVDQSFKEVAFWTPKLGFFE 424

Query: 422 SFVEVNEAAATKLQP---NMGNVAVRDLSRSLMASSSE----EKRLKFAVPGKGSACQEF 481
            FVEVN+   TKL+P   N+GNVAV DL R  + +SSE    EKRL+FAVP +G ACQE 
Sbjct: 425 RFVEVNK-TTTKLKPKFGNLGNVAVGDLPR--LTTSSENCEGEKRLRFAVPEEG-ACQEI 484

Query: 482 VKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGAV 541
           VKVS H  G Y++GFSI+VFRAVM+  N+  PLSYDL PF+GK +DML AV NKTYDGAV
Sbjct: 485 VKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKTYDGAV 544

Query: 542 GDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFI 601
           G+IGI   RF  VDFTV+YLE +IVMVV EK+ +WKQ W F EAF+V+ WLLIP+MHLFI
Sbjct: 545 GEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSQWKQLWAFTEAFDVSTWLLIPTMHLFI 604

Query: 602 SFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASF 661
           SF +WL+ERQN++ELKGFGN++WFS+SIIFYMHREPVKNGLARLVLGPWLF ILV++ASF
Sbjct: 605 SFSVWLLERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGILVVTASF 664

Query: 662 SASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSID 721
           ++SLTSMMT +W++PSVLDV  LK+M+A VGCN+ SFIC YL  T KFEPSKI ++ S++
Sbjct: 665 TSSLTSMMTVTWYRPSVLDVAKLKEMNAVVGCNARSFICDYLEKTLKFEPSKIKRIASLN 724

Query: 722 DYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSA 781
           +YP AF++ +I+AAFFISPHA     K      +   S  +    +AF KGSPLAA VSA
Sbjct: 725 NYPKAFEDDSIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLAAKVSA 784

Query: 782 SIVELTQTNDISQMEQSLLAPLQLQAA 793
           SIVELT+T ++ Q + + L       A
Sbjct: 785 SIVELTETKEMPQFDPNYLVSFNCPTA 801

BLAST of Lag0004270 vs. ExPASy TrEMBL
Match: A0A6J1K353 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111491966 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 1.1e-275
Identity = 531/818 (64.91%), Postives = 642/818 (78.48%), Query Frame = 0

Query: 1   MLRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNHR--NESMRIGVVFDSGSQIGK 60
           +LR   C LS  +GLLLL   L SEA TSK  +NCQ N +  +++ RIGVVFDSGSQ+GK
Sbjct: 4   VLRICFCRLSFILGLLLLSSSLGSEALTSKSILNCQTNPKPNDKTTRIGVVFDSGSQLGK 63

Query: 61  QQMIAMKMALRRFHF-SSCVKLELLFHDYSNPNFTS-TSSAVDLITKGGVKTIVGAVRMQ 120
           QQ++AMKM L  FH  SSC+KLELL HD S+ N TS  SSA+DLITKGGVK +VG+VRMQ
Sbjct: 64  QQLVAMKMGLSHFHLSSSCLKLELLLHD-SHQNLTSPPSSALDLITKGGVKAVVGSVRMQ 123

Query: 121 GLIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANN--FTHRIKCIASILTHFQWRKV 180
            LIVIS+   PV  PIVSTSA+QL+ LKIPSLIQMAN+   THRI+CIASILTHFQWRKV
Sbjct: 124 DLIVISNNNFPVPIPIVSTSAEQLEQLKIPSLIQMANHITITHRIQCIASILTHFQWRKV 183

Query: 181 TLFHEIENIDHP---SSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNN 240
           T+F++I N DH    S++SA+RLFDSLRLA++E+EH L  SSSSNQEILIE+ELK+L N+
Sbjct: 184 TIFYDISNTDHSPIYSAVSANRLFDSLRLADVEVEHRLALSSSSNQEILIEQELKKLMNS 243

Query: 241 QRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEG 300
           QRN VF+VTQL +ELAVL+  +AKK+NMVG NGY WIVS+DVFD IDSLD SS   KMEG
Sbjct: 244 QRNRVFVVTQLEVELAVLVLEQAKKLNMVG-NGYVWIVSNDVFDAIDSLD-SSFWYKMEG 303

Query: 301 VIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEML 360
           VIGFRTYFDDTK SFK FETKFKKMY LEY Q E+P +ASI  VRAYDA++AITRA   +
Sbjct: 304 VIGFRTYFDDTKNSFKSFETKFKKMYRLEYPQEEEPTRASISVVRAYDAARAITRA---M 363

Query: 361 GENFSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLG 420
           GEN S +++ ++I +SNFEGLSGMV+FKNGMLISQSP F+I KV+GQS+K + FWTP LG
Sbjct: 364 GENLSWSEVLEKIGESNFEGLSGMVRFKNGMLISQSPNFKISKVVGQSFKEVGFWTPKLG 423

Query: 421 FVESFVEVNEAAATKLQP---NMGNVAVRDLSRSLMASSSE----EKRLKFAVPGKGSAC 480
           FVE FVEVN+   TKL+P   N+GNVAV DL R  + +SSE    EKRL+FAVP +G AC
Sbjct: 424 FVERFVEVNK-TTTKLKPKFGNLGNVAVGDLPR--LTTSSENCDGEKRLRFAVPEEG-AC 483

Query: 481 QEFVKVSNHSNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYD 540
           QE VKVS H  G Y++GFSI+VFRAVM+  N+  PLSYDL PF+GK +DML AV NKTYD
Sbjct: 484 QEIVKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKTYD 543

Query: 541 GAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMH 600
           GAVG+IGI   RF  VDFTV+YLE +IVMVV EK+ +WKQ W F +AFEV++WLLIP+MH
Sbjct: 544 GAVGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSRWKQLWAFTDAFEVSMWLLIPTMH 603

Query: 601 LFISFVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMS 660
           LFISF +WLIERQN++ELKGFGN++WFS+SIIFYMHREPVKNGLARLVLGPWLF ILV++
Sbjct: 604 LFISFAVWLIERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGILVVT 663

Query: 661 ASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMN 720
           ASF++SLTSMMT SW++PSVLDV  LK+++A VGCN+ SFIC YL  T KFEPSKI +++
Sbjct: 664 ASFTSSLTSMMTVSWYRPSVLDVVKLKEINAVVGCNARSFICDYLKKTLKFEPSKIKRID 723

Query: 721 SIDDYPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAAN 780
           S+++YP AF++ TI+AAFFISPHA     K      +   S  +    +AF KGSPLAA 
Sbjct: 724 SLNNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLAAK 783

Query: 781 VSASIVELTQTNDISQMEQSLLAPLQLQAALQLTKQMG 801
           VSASIVELT+T ++ Q + + L       A +    MG
Sbjct: 784 VSASIVELTETKEMPQFDPNYLVSFNCPTAKEPGPFMG 811

BLAST of Lag0004270 vs. ExPASy TrEMBL
Match: A0A6J1K7S1 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111492435 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 8.1e-266
Identity = 526/794 (66.25%), Postives = 619/794 (77.96%), Query Frame = 0

Query: 11  CFVGLLLLLLLLSSEAHTSKREVNCQKN-HRNESMRIGVVFDSGSQIGKQQMIAMKMALR 70
           CF+GLLLLLLL SSEAHT+K+ +  QKN   N++ RIGVVFDSGSQIGKQQ +AMKM LR
Sbjct: 12  CFLGLLLLLLLPSSEAHTTKKGLKGQKNPTNNKTTRIGVVFDSGSQIGKQQKVAMKMGLR 71

Query: 71  RFHFSSCV-KLELLFHDYSNPNFTS-TSSAVDL-ITKGGVKTIVGAVRMQGLIVISDKKI 130
            FH SS   KLELL HD S+PNFTS TSSAVDL ITKGGVK IVGAV+ Q LIVISDK+I
Sbjct: 72  LFHLSSSSHKLELLLHD-SHPNFTSPTSSAVDLMITKGGVKAIVGAVKRQDLIVISDKEI 131

Query: 131 PVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQW-RKVTLFHEIENIDH 190
               PIVSTSA++L PLKIP LIQMANN TH IKCIASIL +FQ   KV++F++      
Sbjct: 132 LGRIPIVSTSAEKLPPLKIPFLIQMANNITHHIKCIASILKYFQKPPKVSIFYD------ 191

Query: 191 PSSISASRLFDSLRLANIEIEHHLTFSSSSNQ-EILIEKELKRLKNNQRNGVFIVTQLSL 250
           P+  S  RLFDS +LANIE+E+H   SSSSNQ EI IE ELKR+  +QRNGVF+VTQLSL
Sbjct: 192 PTFNSVHRLFDSFQLANIEVENHFALSSSSNQDEISIENELKRVMKSQRNGVFVVTQLSL 251

Query: 251 ELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSL---DSSSLLNKMEGVIGFRTYFDD 310
           EL  LLF KAKKMNMVG NGYTWIVS+DVFDLIDS    D S LLNKMEGVIGFRTYFDD
Sbjct: 252 ELVNLLFIKAKKMNMVG-NGYTWIVSNDVFDLIDSSLINDHSPLLNKMEGVIGFRTYFDD 311

Query: 311 TKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLGEN--FSSTQ 370
           TK  FKRFETKFKKMY+LEY ++E+P KAS   VRAYDA + I RA+E LG+N   SS Q
Sbjct: 312 TKMPFKRFETKFKKMYTLEYPEDEEPIKASSFAVRAYDAVRCIARAMETLGDNNLRSSDQ 371

Query: 371 LTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVESFVEV 430
           + K IL+SNFEG+SGMV+FK+GMLI QSP F+IIKV+ Q YK +AFW+P LGF ESF+EV
Sbjct: 372 VLKTILESNFEGVSGMVRFKDGMLIPQSPNFKIIKVVDQRYKDVAFWSPKLGFSESFLEV 431

Query: 431 NE-AAATKLQPNMG-NVAVRDLSRSLMA----SSSEEKRLKFAVPGKGSACQEFVKVSNH 490
            +  AA K++PNMG +V   DLSR  +     SS EE +LKFAVP KG ACQE VKVS H
Sbjct: 432 EDNKAAIKVKPNMGRHVDGGDLSRKAITLWSQSSGEEGKLKFAVPRKG-ACQELVKVSQH 491

Query: 491 SNGIYISGFSIDVFRAVMN--NMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGAVGDIGIS 550
            NG Y +G SIDVF+A M   NMP   SYDL+PF+G  ++M+ AV+NKTYDGAVGDI I 
Sbjct: 492 LNGNYFTGLSIDVFKAAMTNINMPYSSSYDLYPFDGTYEEMMKAVHNKTYDGAVGDISIM 551

Query: 551 AYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWL 610
           A RF+ VDF+VAYLEADIVMVV E+Q+KWK  WVF EAFEVTVWLLIP++HLFIS VIW+
Sbjct: 552 AQRFESVDFSVAYLEADIVMVVKEEQDKWKSLWVFFEAFEVTVWLLIPTIHLFISSVIWV 611

Query: 611 IERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLTS 670
           IER+NN+ELKG G+++WFS+S+I Y  REPVKNGL+RLVLGPWLF ILV++ SFSASLTS
Sbjct: 612 IERRNNEELKGLGSMLWFSVSLISYTQREPVKNGLSRLVLGPWLFGILVVTESFSASLTS 671

Query: 671 MMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDDYPAAF 730
           MMT SW QP + DV+TLKKMDA+VGCN+ESFIC+YL+ + KFE +KI +MN+IDDYP A 
Sbjct: 672 MMTVSWSQPLIPDVKTLKKMDASVGCNTESFICNYLNGSLKFENAKIKKMNTIDDYPKAL 731

Query: 731 KNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSASIVELT 786
            N +I+AAF I PHA     K      + + S  +G   +AF KGS L  +VSASIVEL 
Sbjct: 732 DNGSIKAAFLIRPHADVFLAKYCIGYTKTNFSIKLGGIGFAFRKGSALGVDVSASIVELI 791

BLAST of Lag0004270 vs. ExPASy TrEMBL
Match: A0A6J1HC77 (glutamate receptor 2.5-like OS=Cucurbita moschata OX=3662 GN=LOC111462756 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 2.0e-264
Identity = 524/798 (65.66%), Postives = 609/798 (76.32%), Query Frame = 0

Query: 1   MLRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNH-RNESMRIGVVFDSGSQIGKQ 60
           +LR   C  SCF+GLLLLLLL S   H       CQ+N   N++ RIG VFDSGSQIGKQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLSSEGLH-------CQRNQPNNKTTRIGAVFDSGSQIGKQ 61

Query: 61  QMIAMKMALRRFHF-SSCVKLELLFHDYSNPNFTSTSSAVDLITKGGVKTIV-GAVRMQG 120
           QM+AMKM LR FH  SSC KLELL HD S+PNF  TSSA+DLIT GGVK +V G+VR Q 
Sbjct: 62  QMVAMKMGLRHFHLSSSCHKLELLLHD-SHPNF--TSSALDLITNGGVKAVVIGSVRAQD 121

Query: 121 LIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQW-RKVTL 180
           LI ISD +IPVG PI+S SA QL P KIPSLIQMANN THR++CI SILTHFQ   KVT+
Sbjct: 122 LIAISDHEIPVGVPILSISATQLHPKKIPSLIQMANNITHRMQCIVSILTHFQLPPKVTV 181

Query: 181 FHEIENIDHP---SSISASRLFDSLRLANIEIEHHLTFSSSSNQ-EILIEKELKRLKNNQ 240
           F+EI NIDHP   SSIS  RLFDS RL N+EI+H L  SSSSNQ EILIE ELK    +Q
Sbjct: 182 FYEITNIDHPSEYSSISVDRLFDSFRLVNVEIDHRLALSSSSNQAEILIENELKSAMKSQ 241

Query: 241 RNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLD-SSSLLNKMEG 300
           RNGVF+VTQLSLELA LLFTKAKK+NMVG NGYTWIVSDDV DLI SLD SSSLL KMEG
Sbjct: 242 RNGVFVVTQLSLELADLLFTKAKKLNMVG-NGYTWIVSDDVLDLIPSLDSSSSLLYKMEG 301

Query: 301 VIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEML 360
           VIGFRTYF+DTKKSFK FETKFKKMY+LEY ++++P KASI  VRAYD  ++I RA++ L
Sbjct: 302 VIGFRTYFNDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAMKTL 361

Query: 361 GEN--FSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPN 420
           G+N   SS QL + IL+SNFEGLSGMV+FKNGMLIS+SP F+IIKV+ QSYK +AFWTP 
Sbjct: 362 GDNNLSSSDQLLETILESNFEGLSGMVRFKNGMLISRSPNFEIIKVVDQSYKPVAFWTPK 421

Query: 421 LGFVESFVEVNEAAATKLQPNMGN-VAVRDLSRSLMASSSE-EKRLKFAVPGKGSACQEF 480
            GF ESFVE N+ +A +   NMGN V VR LS S      + EK+L FAVPG+G ACQEF
Sbjct: 422 SGFAESFVENNKKSALR-SNNMGNMVGVRHLSESFTREDGDGEKQLTFAVPGQG-ACQEF 481

Query: 481 VKVSNHSNGI-YISGFSIDVFRAVMNNMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGAVG 540
           V V    NG  + SGFSI VF  +MNN+    SY    F    +DM+ AVY K YDGAVG
Sbjct: 482 VNVHTFENGTEHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYVKEYDGAVG 541

Query: 541 DIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFIS 600
           DI I A RFQ VDFTVAYL+ DIVMVV EK E+W++ W FM+AF+  VW+LIP+MHLFIS
Sbjct: 542 DITILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLIPTMHLFIS 601

Query: 601 FVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFS 660
            +IWLIER+NN+ELKGFGN++WFS+S+IFYM REPVKNGLARLVLGPWLFAI V++ASFS
Sbjct: 602 SLIWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNGLARLVLGPWLFAIFVVTASFS 661

Query: 661 ASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDD 720
           ASLTSM+T SW QPSV  VE LK+M+ATVGCN+ESFIC+YL DT +FE S I +M S+D+
Sbjct: 662 ASLTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICNYLKDTLQFESSNIKRMESLDN 721

Query: 721 YPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSAS 780
           YP AF++ TI+AAFFISPHA     K      +   S  +G   +AFPKGS  AA VS S
Sbjct: 722 YPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSFKLGGIGFAFPKGSDFAAKVSKS 781

Query: 781 IVELTQTNDISQMEQSLL 785
           I ELT  N+ISQME++LL
Sbjct: 782 IAELTLQNNISQMEKTLL 786

BLAST of Lag0004270 vs. ExPASy TrEMBL
Match: A0A6J1K9A2 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111492270 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 3.1e-257
Identity = 519/806 (64.39%), Postives = 607/806 (75.31%), Query Frame = 0

Query: 1   MLRYSSCCLSCFVGLLLLLLLLSSEAHTSKREVNCQKNH-RNESMRIGVVFDSGSQIGKQ 60
           +LR   C  SCF+GLLLLLLL S   H       CQ+N   N+  RIG VFDSGSQIGKQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLSSEGLH-------CQRNQPNNKPTRIGAVFDSGSQIGKQ 61

Query: 61  QMIAMKMALRRFHF-SSCVKLELLFHDYSNPNFTSTSSAVDLITKGGVKTIV-GAVRMQG 120
           QM+AMKMALR FH  SSC KL+LL HD S+PNF  TSSA+DLIT GGVK +V G+VR Q 
Sbjct: 62  QMVAMKMALRHFHLSSSCHKLKLLLHD-SHPNF--TSSALDLITDGGVKAVVIGSVRTQD 121

Query: 121 LIVISDKKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQW-RKVTL 180
           L+  SD +IPVG PI+S SA QLQP KIPSLIQMANN THR++CIASILTHFQ   KVT 
Sbjct: 122 LVAFSDHEIPVGVPILSISATQLQPKKIPSLIQMANNITHRMQCIASILTHFQLPPKVTF 181

Query: 181 FHEIENIDHP---SSISASRLFDSLRLANIEIEHHLTFSSSSNQ-EILIEKELKRLKNNQ 240
           F+EI N+D P   SSIS  RLFDS R  N+EI+H L  SSSSNQ EILIE ELKR+ N+Q
Sbjct: 182 FYEITNVDRPSEYSSISPDRLFDSFRSVNVEIDHRLALSSSSNQAEILIENELKRVLNSQ 241

Query: 241 RNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLD-SSSLLNKMEG 300
           RNGVF+VTQ+SLELA LLFTKAKKMNMVG NGYTWIVSDDV DLI +LD SSSLL KMEG
Sbjct: 242 RNGVFVVTQISLELADLLFTKAKKMNMVG-NGYTWIVSDDVLDLIPTLDSSSSLLYKMEG 301

Query: 301 VIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEML 360
           VIGFRTYF+DTKKSFK FET+FKKMY+LEY +++ P KASI  VRAYDA ++I RA+E L
Sbjct: 302 VIGFRTYFNDTKKSFKSFETRFKKMYNLEYPEDKDPIKASIFAVRAYDAVRSIARAMETL 361

Query: 361 GEN--FSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPN 420
           G+N   SS QL + IL+SNFEGLSGMV+FKNGMLISQSP F+IIKV+ QSYK +AFWTP 
Sbjct: 362 GDNNLSSSDQLLETILESNFEGLSGMVRFKNGMLISQSPNFEIIKVVDQSYKPVAFWTPK 421

Query: 421 LGFVESFVEVNEAAATKLQPNMGN-VAVRDLSRSLMASSSE-EKRLKFAVPGKGSACQEF 480
            GF ESF E N+ +A +   NMG+ V VR LS S      + EK L FAVPG+G AC EF
Sbjct: 422 SGFAESFEENNKKSALR-SNNMGSMVGVRHLSESFTREDGDGEKHLTFAVPGQG-ACHEF 481

Query: 481 VKVSNHSNGI-YISGFSIDVFRAVMNNMPGPLSYDLFPFEGKNDDMLHAVYNKTYDGAVG 540
           V +    NG  + SGFSI VF  VM N+    S+    F    +DM+ AVY K YDGAVG
Sbjct: 482 VNMHTFENGTEHFSGFSIAVFNEVMKNIKNMPSFKFARFNHSYNDMIDAVYRKEYDGAVG 541

Query: 541 DIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFIS 600
           DI I A RFQ VDFTVAYL+ DIVMVV EK    ++ W FM+AF+  VW+LIP+MHLFIS
Sbjct: 542 DITILAKRFQQVDFTVAYLKTDIVMVVREKY--GRKLWAFMDAFKPEVWVLIPTMHLFIS 601

Query: 601 FVIWLIERQNNDELKGFGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFS 660
            +IWLIER+NN+ELKGFGN++WFS+S+IFY  REPVKNGLARLVLGPWLFAI +++ASFS
Sbjct: 602 SLIWLIERKNNEELKGFGNMLWFSVSLIFYTQREPVKNGLARLVLGPWLFAIFMVTASFS 661

Query: 661 ASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDD 720
           ASLTSM+T SW QPSV  VE LK+M+ATVGCN+ESFIC YL+ + KFE ++I +MNSIDD
Sbjct: 662 ASLTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICDYLNGSLKFENARIKKMNSIDD 721

Query: 721 YPAAFKNRTIEAAFFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSAS 780
           YP AF+N +I AAFFISPHA     K  +   +   S  +G   +AFPKGS LAA VS S
Sbjct: 722 YPKAFENGSITAAFFISPHADVFLVKNCKRYTKGVSSFKLGGIGFAFPKGSDLAAKVSKS 781

Query: 781 IVELTQTNDISQMEQSLLAPLQLQAA 793
           I ELT  N+ISQME++LL   Q  +A
Sbjct: 782 IAELTLQNNISQMEKTLLNSFQCPSA 792

BLAST of Lag0004270 vs. TAIR 10
Match: AT2G29100.1 (glutamate receptor 2.9 )

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-69
Identity = 215/781 (27.53%), Postives = 380/781 (48.66%), Query Frame = 0

Query: 39  HRNESMRIGVVFDSGSQIGKQQMIAMKMALRRF---HFSSCVKLELLFHDYSNPNFTSTS 98
           ++   +++GVV D  +   K  + ++KMA+  F   H +   +L L   D       +++
Sbjct: 25  NQTSEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPNYLTRLTLHVRDSMEDTVQASA 84

Query: 99  SAVDLITKGGVKTIVGAVR-MQGLIVIS-DKKIPVGTPIVSTSAKQLQPLKIPSLIQMAN 158
           +A+DLI    V  I+G +  MQ   +I    K  V T   S ++  L  +K P  ++   
Sbjct: 85  AALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTITFSATSPLLTSIKSPYFVRATI 144

Query: 159 NFTHRIKCIASILTHFQWRKVTLFHEIENIDHPSSISASRLFDSLRLANIEIEHHLTFSS 218
           + + +++ IASI   F+WR+V   + ++N      +    LFD+L+  ++E++  +    
Sbjct: 145 DDSSQVRAIASIFKFFRWRRVVAIY-VDNEFGEGFMPF--LFDALQ--DVEVKRSVIPPE 204

Query: 219 SSNQEILIEKELKRLKNNQRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWIVSDDV 278
           + + E  I+KEL++L   Q   VF+V  +   LA+ +F  A+ + M+   GY W++++ +
Sbjct: 205 AIDDE--IQKELRKLMERQAR-VFVV-HMESSLALRVFQIARDIGMM-EEGYVWLMTNGM 264

Query: 279 FDLIDSLDSSSLLNKMEGVIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPRKASIL 338
             ++  +++   LN +EGV+G R++   +K+    F  ++K+ +  E +        ++ 
Sbjct: 265 THMMRHINNGRSLNTIEGVLGVRSHVPKSKE-LGDFRLRWKRTF--EKENPSMRDDLNVF 324

Query: 339 EVRAYDASQAITRAVEM-----------------------LGENFSSTQLTKQILKSNFE 398
            + AYD+  A+ +AVE                        +G +     L K   +  F 
Sbjct: 325 ALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSEVRFN 384

Query: 399 GLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVESFVEVNEAAATKLQPN 458
           GL+G  K  +G L  QSPKF+II  +G   + I FWTP  G +++     +     + P 
Sbjct: 385 GLAGEFKLIDGQL--QSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLGPVIWPG 444

Query: 459 MGNVAVRDLSRSLMASSSEEKRLKFAVPGKGSACQEFVKVSNH--SNGIYISGFSIDVFR 518
              +  +             K+L+  VP K     +FVKV+ +  +N    +G++I++F 
Sbjct: 445 KSKIVPK-------GWEIPGKKLRVGVPMK-KGFFDFVKVTINPITNKKTPTGYAIEIFE 504

Query: 519 AVMNNMPGPLSYDLFPFEGKN--DDMLHAVYNKTYDGAVGDIGISAYRFQHVDFTVAYLE 578
           A +  +P  +  +   FE  N  +++++ VY+KT+D  VGDI I+A R  + DFT+ + E
Sbjct: 505 AALKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTE 564

Query: 579 ADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWLIERQNNDELKG---- 638
           + + M+V  +  + K  WVF+E + + +W+      +FI FV+WL E + N + +G    
Sbjct: 565 SGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQY 624

Query: 639 -FGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLTSMMTKSWFQPS 698
             G  +WFS S + + HRE V + LAR V+  W F +LV++ S++ASLTS +T    QP+
Sbjct: 625 QIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPT 684

Query: 699 VLDVETLKKMDATVGCNSESFICSYLHDT--HKFEPSKIVQMNSIDDYPAAFKNRTIEAA 758
           V +V  L K    VG    +F+   L     H+ +          DD  +  K++ I AA
Sbjct: 685 VTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFDSAKDADDLLSKGKSKGIAAA 744

Query: 759 FFISPHAKFSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSASIVELTQTNDISQM 781
           F    + K    +          +   G   +AFPK SPL    S +I+ LTQ N   Q+
Sbjct: 745 FDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQI 782

BLAST of Lag0004270 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 252.3 bits (643), Expect = 1.3e-66
Identity = 212/787 (26.94%), Postives = 377/787 (47.90%), Query Frame = 0

Query: 35  CQKNHRNESMRIGVVFDSGSQIGKQQMIAMKMALR---RFHFSSCVKLELLFHDYSNPNF 94
           C   ++   +++GVV D  +   K  + ++ ++L    ++H     +L +   D      
Sbjct: 30  CLGQNQTTEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYHSDYTTRLAIHIRDSMEDVV 89

Query: 95  TSTSSAVDLITKGGVKTIVG---AVRMQGLIVISDKKIPVGTPIVSTSAKQLQPLKIPSL 154
            ++S+A+DLI    V  I+G   +++ + +I ++DK   V T   S +   L  +  P  
Sbjct: 90  QASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKS-QVPTITFSATCPLLTSINSPYF 149

Query: 155 IQMANNFTHRIKCIASILTHFQWRKVTLFHEIENIDHPSSISASRLFDSLRLANIEIEHH 214
           ++   + + ++K IA+I+  F WR V   + ++N      +    L D+L+     + + 
Sbjct: 150 VRATLDDSSQVKAIAAIVKSFGWRNVVAIY-VDNEFGEGILPL--LTDALQDVQAFVVNR 209

Query: 215 LTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQLSLELAVLLFTKAKKMNMVGNNGYTWI 274
                 +N + ++ KEL +L   Q   VF+V  +   L    F KA+++ M+   GY W+
Sbjct: 210 CLIPQEANDDQIL-KELYKLMTMQTR-VFVV-HMPPTLGFRFFQKAREIGMM-EEGYVWL 269

Query: 275 VSDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDDTKKSFKRFETKFKKMYSLEYQQNEQPR 334
           ++D V +L+ S +  S L  M+GV+G R++   +KK  K F  +++KM+  +    E   
Sbjct: 270 LTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKK-LKNFRLRWEKMFPKKGNDEEM-- 329

Query: 335 KASILEVRAYDASQAITRAVE-----------------------MLGENFSSTQLTKQIL 394
             +I  +RAYD+  A+  AVE                        LG +     L K + 
Sbjct: 330 --NIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALS 389

Query: 395 KSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFVES-FVEVNEAAA 454
              F GL+G  +  NG L  +S  F +I ++G   + I  W P+ G V +          
Sbjct: 390 NVRFNGLAGEFELINGQL--ESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSVLG 449

Query: 455 TKLQPNMGNVAVRDLSRSLMASSSEEKRLKFAVPGKGSACQEFV--KVSNHSNGIYISGF 514
            +L P +     +D+ +     ++  K L+  +P K     EFV  K+   SN +  +G+
Sbjct: 450 ERLGPVIWPGKSKDVPKGWQIPTN-GKMLRVGIPVK-KGFLEFVDAKIDPISNAMTPTGY 509

Query: 515 SIDVFRAVMNNMPGPL--SYDLFPFEGKN-DDMLHAVYNKTYDGAVGDIGISAYRFQHVD 574
            I++F AV+  +P  +   Y  F    +N D+M++ VY   YD  VGD+ I A R  +VD
Sbjct: 510 CIEIFEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVD 569

Query: 575 FTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWLIERQNNDE 634
           FT+ Y E+ + M+V  K    K  WVF+  + + +W+      +FI F++W++E + N +
Sbjct: 570 FTLPYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTD 629

Query: 635 LKG-----FGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLTSMMT 694
            +G      G   WF+ S + + HRE V + LAR V+  W F +LV+  S++A+LTS  T
Sbjct: 630 FRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFT 689

Query: 695 KSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDDYPAAFKNR 754
               QP+V + + L K +  +G    +F+   L  +  F+ S++    S  +    F N 
Sbjct: 690 VKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELL-KSQGFDESQLKPFGSAVECDELFSNG 749

Query: 755 TIEAAFFISPHAK-FSSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSASIVELTQT 781
           TI A+F    + K   S+  ++ T  +    + G   + FPK SPL  +VS +I+ +TQ 
Sbjct: 750 TITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFG-FVFPKKSPLTDDVSRAILNVTQG 795

BLAST of Lag0004270 vs. TAIR 10
Match: AT2G29110.1 (glutamate receptor 2.8 )

HSP 1 Score: 245.7 bits (626), Expect = 1.2e-64
Identity = 225/818 (27.51%), Postives = 392/818 (47.92%), Query Frame = 0

Query: 9   LSCFVGLLLLLLLLSSEAHTSKREVNCQKNHRNESMRIGVVFDSGSQIGKQQMIAMKMAL 68
           LS FV L LLL            EV   +N  +E +++GVV D  +   K  + ++ +AL
Sbjct: 11  LSYFVCLFLLL------------EVGLGQNQISE-IKVGVVLDLNTTFSKICLTSINLAL 70

Query: 69  RRF---HFSSCVKLELLFHDYSNPNFTSTSSAVDLITKGGVKTIVGAV-RMQGLIVIS-D 128
             F   H +   +L L   D       ++++A+DLI    V  I+G +  MQ   +I   
Sbjct: 71  SDFYKDHPNYRTRLALHVRDSMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLA 130

Query: 129 KKIPVGTPIVSTSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLFHEIENI 188
            K  V T   S ++  L  +K    ++   + ++++K IA+I   F WR V   +    +
Sbjct: 131 NKTQVPTISFSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNEL 190

Query: 189 DHPSSISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQLS 248
                     LFD+L+  +++++  +  S +++ +IL  KEL +L   Q   VF+V  ++
Sbjct: 191 GEG---IMPYLFDALQ--DVQVDRSVIPSEANDDQIL--KELYKLMTRQTR-VFVV-HMA 250

Query: 249 LELAVLLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDDTK 308
             LA  +F KA ++ M+   GY W++++ +  ++  +     LN ++GV+G R++   + 
Sbjct: 251 SRLASRIFEKATEIGMM-EEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKS- 310

Query: 309 KSFKRFETKFKKMYSLEYQQNEQPR-KASILEVRAYDASQAITRAVEMLGENFSS----- 368
           K  + F  ++K+ +    ++N   R   SI  + AYD++ A+  AVE    N SS     
Sbjct: 311 KGLEDFRLRWKRNFK---KENPWLRDDLSIFGLWAYDSTTALAMAVEK--TNISSFPYNN 370

Query: 369 --------------------TQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMG 428
                                 L + + +  F GL+G     +  L  +SPKF+II  +G
Sbjct: 371 ASGSSNNMTDLGTLHVSRYGPSLLEALSEIRFNGLAGRFNLIDRQL--ESPKFEIINFVG 430

Query: 429 QSYKGIAFWTPNLGFVESFVEVNEAAATKLQPNMGNVAVRDLSRSLMASSSE----EKRL 488
              + + FWTP+ G     V VN    T          +     +++    E     K++
Sbjct: 431 NEERIVGFWTPSNG----LVNVNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNGKKI 490

Query: 489 KFAVPGKGSACQEFVKVSNH--SNGIYISGFSIDVFRAVMNNMPGPLSYDLFPFEGKN-- 548
           K  VP K      FV+V     +N     G++ID+F A +  +P  +    + FE  +  
Sbjct: 491 KVGVPVK-KGFFNFVEVITDPITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPDDD 550

Query: 549 -DDMLHAVYNKTYDGAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFME 608
            DD+++ V N T D  VGD+ I+AYR  + DFT+ Y E+ + M+V  +  + K  WVF++
Sbjct: 551 YDDLVYKVDNGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLK 610

Query: 609 AFEVTVWLLIPSMHLFISFVIWLIERQNNDELKG-----FGNLVWFSISIIFYMHREPVK 668
            + + +W+      + I FV+WL E + N + +G      G   WFS S + + HRE V 
Sbjct: 611 PWGLDLWVTTACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVV 670

Query: 669 NGLARLVLGPWLFAILVMSASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFI 728
           + LAR V+  W F +LV++ S++A+LTS +T   FQP+ ++V+ L K    VG    +F+
Sbjct: 671 SNLARFVVVVWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFV 730

Query: 729 CSYLHDTHKFEPSKIVQMNSIDDYPAAFKNRTIEAAFFISPHAK-FSSKKIAEATPRQSL 781
             +L     F  SK+    S ++  A   N +I AAF    + +   S+  ++    +  
Sbjct: 731 KDFL-IKEGFNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLRAILSQYCSKYAIVEPT 790

BLAST of Lag0004270 vs. TAIR 10
Match: AT5G11210.1 (glutamate receptor 2.5 )

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-64
Identity = 201/699 (28.76%), Postives = 355/699 (50.79%), Query Frame = 0

Query: 131 PIVSTSAKQ--LQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLFHEIENIDHPSS 190
           PI+S SA    L  L+ P  I+  ++ + +++ I++I+  F+WR+V   + ++N +    
Sbjct: 53  PIISFSATSPLLDSLRSPYFIRATHDDSSQVQAISAIIESFRWREVVPIY-VDN-EFGEG 112

Query: 191 ISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQLSLELAV 250
           I    L D+ +  N+ I +    S   + +  I+KEL +L       VFIV  L  +L  
Sbjct: 113 I-LPNLVDAFQEINVRIRYRSAISLHYSDD-QIKKELYKLM-TMPTRVFIVHMLP-DLGS 172

Query: 251 LLFTKAKKMNMVGNNGYTWIVSDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDDTKKSFKR 310
            LF+ AK+++M+ + GY WIV++ + DL+  +  SSL+N M GV+G +TYF  +K+    
Sbjct: 173 RLFSIAKEIDML-SKGYVWIVTNGIADLMSIMGESSLVN-MHGVLGVKTYFAKSKE-LLH 232

Query: 311 FETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVE------------------- 370
            E +++K +  E   N            AYDA+ A+  +VE                   
Sbjct: 233 LEARWQKRFGGEELNN--------FACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRD 292

Query: 371 -------MLGENFSSTQLTKQILKSNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKG 430
                   LG   S  +L   +   +F+G++G  + KNG L  ++  F+II +     + 
Sbjct: 293 DIGTDLDELGVALSGPKLLDALSTVSFKGVAGRFQLKNGKL--EATTFKIINIEESGERT 352

Query: 431 IAFWTPNLGFVESF-VEVNEAAATKLQPNMGNVAVRDLSRSLMASSSEEKRLKFAVPGKG 490
           + FW   +G V+S  V+    ++ +L+P +       + +      +  K+L+ AVP K 
Sbjct: 353 VGFWKSKVGLVKSLRVDKVSHSSRRLRPIIWPGDTIFVPKG-WEFPTNAKKLRIAVP-KK 412

Query: 491 SACQEFVKVS--NHSNGIYISGFSIDVFRAVMNNMPGPLSYDLFPFE-------GKNDDM 550
                FV+V+   ++N   ++GF IDVF  VM+ MP  +SY+  PF+       G  D+M
Sbjct: 413 DGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKPRGSYDEM 472

Query: 551 LHAVYNKTYDGAVGDIGISAYRFQHVDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEV 610
           ++ V+   +DGAVGD  I A R  +VDF + Y E  IV +V  K  K K  WVF++    
Sbjct: 473 VYNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWVFLKPLTK 532

Query: 611 TVWLLIPSMHLFISFVIWLIERQNNDELK------GFGNLVWFSISIIFYMHREPVKNGL 670
            +WL+  +  L+I  ++W+ E Q ++E +         ++ +FS S +F+ HR P ++  
Sbjct: 533 ELWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHRRPSESFF 592

Query: 671 ARLVLGPWLFAILVMSASFSASLTSMMTKSWFQPSVLDVETLKKMDATVGCNSESFICSY 730
            R+++  W F +L+++ S++A+LTSM+T    +P+V  ++ L+K    +G  + SF    
Sbjct: 593 TRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQTGSFTFER 652

Query: 731 LHDTHKFEPSKIVQMNSIDDYPAAF----KNRTIEAAFFISPHAK-FSSKKIAEATPRQS 781
           L    +F+ S++   NS ++    F     N  I+AAF    + K F +K  +E +  + 
Sbjct: 653 LKQM-RFDESRLKTYNSPEEMRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCSEYSIIEP 712

BLAST of Lag0004270 vs. TAIR 10
Match: AT5G48400.2 (Glutamate receptor family protein )

HSP 1 Score: 234.2 bits (596), Expect = 3.7e-61
Identity = 216/792 (27.27%), Postives = 360/792 (45.45%), Query Frame = 0

Query: 13  VGLLLLLLLLSSEAHTSKREVNCQKNHRNESMRIGVVFDSGSQIGKQQMIAMKMALRRF- 72
           +  LL+LL   S    S +  +  K  R   +R+G+V D GS  GK    ++ MAL  F 
Sbjct: 12  LSFLLVLLFFISNCFASSQNNDDDKRIR---VRVGLVLDLGSVEGKIVRSSVSMALSDFY 71

Query: 73  --HFSSCVKLELLFHDYSNPNFTSTSSAVDLITKGGVKTIVGAVRMQGLIVISDKKIPVG 132
             H     +L LL  D       +  S VDL+   GV+ I+G   +    ++++      
Sbjct: 72  DNHNDYKTRLSLLVRDSHGEPLLALDSVVDLLQTEGVQAIIGGNSLLEAKLLAELGEKAR 131

Query: 133 TPIVS-TSAKQLQPLKIPSLIQMANNFTHRIKCIASILTHFQWRKVTLFHEIENIDHPS- 192
            P++S  S   L   K   LIQ  +N    +K I + L  F W  V L  E    DH   
Sbjct: 132 VPVISLNSPMSLSLSKYTHLIQATHNSASEVKGITAFLHGFDWNSVALVLE----DHDDW 191

Query: 193 SISASRLFDSLRLANIEIEHHLTFSSSSNQEILIEKELKRLKNNQRNGVFIVTQLSLELA 252
             S   + D     N+ ++  + FS +S+++ L+++ L+ LK +    VF+V  LS  +A
Sbjct: 192 RESMHFMVDHFHENNVHVQSKVAFSVTSSEDSLMDR-LRELK-DLGTTVFVV-HLSEVIA 251

Query: 253 VLLFTKAKKMNMVGNNGYTWIV-SDDVFDLIDSLDSSSLLNKMEGVIGFRTYFDDTKKSF 312
             LF  A+K+ M+G  G+ WI+ S  +    D          MEGV+GF++Y     K  
Sbjct: 252 TRLFPCAEKLGMMG-EGFAWILTSRSMSSFHDQFIDDLTKEAMEGVVGFKSYI-PMSKEL 311

Query: 313 KRFETKFKKMYSLEYQQNEQPRKASILEVRAYDASQAITRAVEMLGENFSSTQLTKQILK 372
             F  +++K   +E     +  + SI  V A+D + ++  A E+      ++ L + I +
Sbjct: 312 HNFTLRWRKTLPVEEVTGSEITRLSISGVWAHDVAWSLASAAEVTRMPTVTSTLLEAIKE 371

Query: 373 SNFEGLSGMVKFKNGMLISQSPKFQIIKVMGQSYKGIAFWTPNLGFV--ESFVEVNEAAA 432
           S F+GLSG  +  +  L+S   KF+I+ ++G   + + FW  N  F         ++   
Sbjct: 372 SRFKGLSGNFQLDDMKLLSD--KFEIVNMIGSGERRVGFWNSNGSFSNRRQLSSTHDNLE 431

Query: 433 TKLQPNMGNVAVRDLSRSLMASSSEEKRLKFAVPGKGSACQEFVKVSNHSNGIYISGFSI 492
           T + P  G  A     RSL  S  ++ R+      +     +            + GF I
Sbjct: 432 TIIWP--GGSAQSPKGRSLRESGRKKLRVLVTSSNRFPRLMKVETDPITHEITIVEGFCI 491

Query: 493 DVFRAVMNNMPGPLSYDLFPFEGKNDD-------MLHAVYNKTYDGAVGDIGISAYRFQH 552
           +VF+A +     P +Y++      N          LH+  +K YD AVGDI I++ R  +
Sbjct: 492 EVFQASI----APFNYEVEYIRWLNGTNYTKLAYALHSQKDK-YDAAVGDITITSDRSMY 551

Query: 553 VDFTVAYLEADIVMVVTEKQEKWKQFWVFMEAFEVTVWLLIPSMHLFISFVIWLIERQNN 612
           VDFT+ Y E  + +V      K +  WVF +     +W+   +  +    ++WLIER  N
Sbjct: 552 VDFTLPYTEMGLGIVAA----KERSMWVFFQPLTPNLWITSAAFFVLTGIIVWLIERAEN 611

Query: 613 DELKG-----FGNLVWFSISIIFYMHREPVKNGLARLVLGPWLFAILVMSASFSASLTSM 672
            E +G      G ++WF  S + Y HRE +++ L+R V+  W+FA+L++  S++A+LTSM
Sbjct: 612 KEFQGSWPQQIGVVIWFGFSTLVYAHREKLQHNLSRFVVTVWVFAVLILVTSYTATLTSM 671

Query: 673 MTKSWFQPSVLDVETLKKMDATVGCNSESFICSYLHDTHKFEPSKIVQMNSIDDYPAAFK 732
           MT    +            +  VG  S S I +           +++ +N+ +DY  A  
Sbjct: 672 MTVQQIR--------FNANEDYVGHLSGSLIANAALTNSSLRAMRLLGLNTSEDYAQALM 731

Query: 733 NRTIEAAFFISPHAKF---SSKKIAEATPRQSLSSSVGLALWAFPKGSPLAANVSASIVE 782
           N+++       P+ K     +         QS ++  G   + F KGS LA NVS  I +
Sbjct: 732 NKSVSYIVSELPYLKILLGENPGHFLMVKTQSTTNGFG---FMFQKGSELAPNVSREIAK 767