Lag0004243 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004243
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionSASA domain-containing protein
Locationchr6: 2208903 .. 2211619 (-)
RNA-Seq ExpressionLag0004243
SyntenyLag0004243
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGTTGAAACTATCGATCTTGCTATGTACAATGCTATTTGGCCCTTCCCTTTCTGGGGCTACTTCTCCTCAAAACATATTCATCCTAGCTGGTCAAAGCAACATGGCTGGCCGAGGAGGGGTTGAGAAAGATCACTCGGGGGTACTTGTGTGGGATGGGTATGTCCCACCAGAGGCTCAACCCAACCCATCCATCCTACGATTAAGCCCAGAACGCCAATGGGAGATAGCACTAGAGCCTGTCCATGATGGGATCGACATCAATAAAACAGTTGGTGTTGGTCCAGCAATGGCATTTGCTCACCACCTCCAAGCAAAAGCTGGAACCAAAGTTGGCGTTGTGGGTTTAGTTCCTTGTGCTAGAGGTGGCACTTTGATCGAACAATGGATTAAAAACCCTAACAACCCTAATGCAACTTTTTACCAAAATTTCATTGAAAGAATCAAAGCATCAGATGAAAAAGGTGGAGTGGTTCGAGCACTTTTCTGGTTGCAAGGAGAAAGCGATGCGGCTGCGAGTGACACTGCTAGTAGATACAAAAACAACCTAAAGAAGTTCTTCACAGACATCCGCAATGATATAAAGCCTAGATTTTTACCTATCATTCTTGTTAAAATAGCCGTTTATGACACCTTTTTGAAGCATGATACTCATGATTTGCCTTCAGTAAGGGCCGCAGAAGATGCAGTCCAGAGGGAGCTTCCGGATGTGGTGACCATCGACTCCTTGAAATTGGTGAACACTAGAACACATGAAGGCTTTAACCAGGATCATGGTCATTTTAATATCAAGACTGAAATTGCTTTGGGCAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTATGATAAATTTTTTCTTCTTTAATCTCTACAACTCTCTCATTCTATCTAAATTAATAAATCATCTTTTCCACCTCTTTCTAGAGTTTATCTATATTCAATTCTTTAGATGATTAATATTCCAGATCATTTCAACAAATAAATTAAATAATTATAAGCGGCTTGAAATATAAATTATGATGAGATAAGGTTTTTTTACCATAATTAATGGTTAAAAAAGTACTTTATAGAAAATATCAATTTAGTTCTTAAATATATAACAATTTAGATGCTATATACTATAAATGATATACAACTATCCTAGGTTGGTCTAGTAGTCAATAAGGGTTATAGAAAAAATAATGTGTTTAAAGAGACGTCTTTTTAAAACGTTGGTGGGAGTCTTTTATTATTTAAAAAGAACTAACCACATCTCAATGTATATTTTATTATATTATGTTTTATCTATAAATAATCGAACTATGTTATAAAAGTTAAAGGTCCCACAAGCTCGATTTGGTTTGGATCGGAGTATTAATCTCCCCATTCATTCTTTCACATGGCCTCTGCAAACATTTCTTAACCACTCGGCTAAGGTTTCTTTCTTTGTCATCTCAGCTCCACTACTCCACTTCCCACCCTGAAATGTGTCTTGCTAGAGAGAAATTTCAAGAAAAATGTATTCATAAGTCATAACCCATAATTATATTATTTATCAAAGATAACTCTATTAGGAAGAAATAATGCATATTGAGATTTTGAAAGGTGTTACAACAAGCAATCCAAGTTAAAGTATTTTCATGGTTAGATTTGGACATCTATATCATATTTTTAGGCATTTTGTTAGTTGGATTTATAATCTACTGGTTGATTCATGGACAATTTTCTAATTTAATTTTAAGGAAATATTATAATTTGCAACACTTTATTTAAGTTCAAAATTTTAATGGATGAAATCTAAAAGTTTAAGGTTAAACCCGATAGAATGAATTTATGTTAGGGTAAGAAAAGAATTTTCTGGAAATAAAATTTGATAAAGAATAAAATATAGGGCCAAAAGGGATTTAAATACATTTTCGGATTGGATGCTCCTAACATATATCAATTATCATCATAAGCCTTATCTCGTGCATAATAATTTATATTCCAAACACCTTCCGGACATTCTTTAATTATTGCTTTAATATTTAAACATTACCAACTGTGAAGGAAAAAAAAAAAAAAAATCAGTGTTTCATTTGGAGCAATATTTTTTTGTTGGTTTGGTAGCTGCCTATATATAAAATAAAACAAAGAGCATTAGCTAGTTTTAACAACAATGGCTGTCGTTTTGTTTTTTACTCTTTTGCTTTTATCTAGATGAAACAACTTGCTCTAATTTATAAACGAGGAGAGATTTCACCTCTTTTTTACTTCTTTTCCTTTTTTACAAAAATATCCATTTTTTTAGTTCAACATGTGAGCTAAGGAAGATTCCAACCTTTTAATGGTCTGAAGTATATATATTTTAACTAATTGAATTATGCTTCCAACAATCTATCGGAGGCATCAACCATCTTAGCGATGCAACTTGGAATGCTCGAACGAGTAGCGGCGGGATCGGCTGGATTCTTCGACGGCAAGATGGTACTCCAGTGACAGCTGGGTTCCGATTAATCAAGGAAAATTGGAAAATCAGTTGGTTGGAAGCCGCTCTGGTGGAGGGGCTGAAATCGACTCCATTTTTTTCTCCCAAGTTGGTTATCGAGCTTGACTCAGTACAAGTGGTACACCTGCTTGAGGAGAAAGAAGTCGACCTCACAGAACTCGGGCTTTTCATTAATGAAGCGAAGCTTTTACTCTCCAACTTCCAAGTTCACTTGATTTCCCAGGAGGCATAA

mRNA sequence

ATGGCTTTGTTGAAACTATCGATCTTGCTATGTACAATGCTATTTGGCCCTTCCCTTTCTGGGGCTACTTCTCCTCAAAACATATTCATCCTAGCTGGTCAAAGCAACATGGCTGGCCGAGGAGGGGTTGAGAAAGATCACTCGGGGGTACTTGTGTGGGATGGGTATGTCCCACCAGAGGCTCAACCCAACCCATCCATCCTACGATTAAGCCCAGAACGCCAATGGGAGATAGCACTAGAGCCTGTCCATGATGGGATCGACATCAATAAAACAGTTGGTGTTGGTCCAGCAATGGCATTTGCTCACCACCTCCAAGCAAAAGCTGGAACCAAAGTTGGCGTTGTGGGTTTAGTTCCTTGTGCTAGAGGTGGCACTTTGATCGAACAATGGATTAAAAACCCTAACAACCCTAATGCAACTTTTTACCAAAATTTCATTGAAAGAATCAAAGCATCAGATGAAAAAGGTGGAGTGGTTCGAGCACTTTTCTGGTTGCAAGGAGAAAGCGATGCGGCTGCGAGTGACACTGCTAGTAGATACAAAAACAACCTAAAGAAGTTCTTCACAGACATCCGCAATGATATAAAGCCTAGATTTTTACCTATCATTCTTGTTAAAATAGCCGTTTATGACACCTTTTTGAAGCATGATACTCATGATTTGCCTTCAGTAAGGGCCGCAGAAGATGCAGTCCAGAGGGAGCTTCCGGATGTGGTGACCATCGACTCCTTGAAATTGGTGAACACTAGAACACATGAAGGCTTTAACCAGGATCATGGTCATTTTAATATCAAGACTGAAATTGCTTTGGGCAAATGGTTGGCTGATACCTACCTCTCCCACTATGGCATCAACCATCTTAGCGATGCAACTTGGAATGCTCGAACGAGTAGCGGCGGGATCGGCTGGATTCTTCGACGGCAAGATGGTACTCCAGTGACAGCTGGGTTCCGATTAATCAAGGAAAATTGGAAAATCAGTTGGTTGGAAGCCGCTCTGGTGGAGGGGCTGAAATCGACTCCATTTTTTTCTCCCAAGTTGGTTATCGAGCTTGACTCAGTACAAGTGGTACACCTGCTTGAGGAGAAAGAAGTCGACCTCACAGAACTCGGGCTTTTCATTAATGAAGCGAAGCTTTTACTCTCCAACTTCCAAGTTCACTTGATTTCCCAGGAGGCATAA

Coding sequence (CDS)

ATGGCTTTGTTGAAACTATCGATCTTGCTATGTACAATGCTATTTGGCCCTTCCCTTTCTGGGGCTACTTCTCCTCAAAACATATTCATCCTAGCTGGTCAAAGCAACATGGCTGGCCGAGGAGGGGTTGAGAAAGATCACTCGGGGGTACTTGTGTGGGATGGGTATGTCCCACCAGAGGCTCAACCCAACCCATCCATCCTACGATTAAGCCCAGAACGCCAATGGGAGATAGCACTAGAGCCTGTCCATGATGGGATCGACATCAATAAAACAGTTGGTGTTGGTCCAGCAATGGCATTTGCTCACCACCTCCAAGCAAAAGCTGGAACCAAAGTTGGCGTTGTGGGTTTAGTTCCTTGTGCTAGAGGTGGCACTTTGATCGAACAATGGATTAAAAACCCTAACAACCCTAATGCAACTTTTTACCAAAATTTCATTGAAAGAATCAAAGCATCAGATGAAAAAGGTGGAGTGGTTCGAGCACTTTTCTGGTTGCAAGGAGAAAGCGATGCGGCTGCGAGTGACACTGCTAGTAGATACAAAAACAACCTAAAGAAGTTCTTCACAGACATCCGCAATGATATAAAGCCTAGATTTTTACCTATCATTCTTGTTAAAATAGCCGTTTATGACACCTTTTTGAAGCATGATACTCATGATTTGCCTTCAGTAAGGGCCGCAGAAGATGCAGTCCAGAGGGAGCTTCCGGATGTGGTGACCATCGACTCCTTGAAATTGGTGAACACTAGAACACATGAAGGCTTTAACCAGGATCATGGTCATTTTAATATCAAGACTGAAATTGCTTTGGGCAAATGGTTGGCTGATACCTACCTCTCCCACTATGGCATCAACCATCTTAGCGATGCAACTTGGAATGCTCGAACGAGTAGCGGCGGGATCGGCTGGATTCTTCGACGGCAAGATGGTACTCCAGTGACAGCTGGGTTCCGATTAATCAAGGAAAATTGGAAAATCAGTTGGTTGGAAGCCGCTCTGGTGGAGGGGCTGAAATCGACTCCATTTTTTTCTCCCAAGTTGGTTATCGAGCTTGACTCAGTACAAGTGGTACACCTGCTTGAGGAGAAAGAAGTCGACCTCACAGAACTCGGGCTTTTCATTAATGAAGCGAAGCTTTTACTCTCCAACTTCCAAGTTCACTTGATTTCCCAGGAGGCATAA

Protein sequence

MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPEAQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYGINHLSDATWNARTSSGGIGWILRRQDGTPVTAGFRLIKENWKISWLEAALVEGLKSTPFFSPKLVIELDSVQVVHLLEEKEVDLTELGLFINEAKLLLSNFQVHLISQEA
Homology
BLAST of Lag0004243 vs. NCBI nr
Match: XP_022131651.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-139
Identity = 245/284 (86.27%), Postives = 267/284 (94.01%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSI+LC MLFGPSLSGATSP+NIFILAGQSNMAGRGGVEK+ +G L WDGYVPPE
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           +QP+PSILRL+PERQWE+A EPVH GIDI KTVGVGPA+AFAH LQAK G+KVG VGLVP
Sbjct: 61  SQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+NPNATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+SDTA R
Sbjct: 121 CARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAER 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPR LPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TID+LKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS+YG
Sbjct: 241 TIDALKLVNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYG 284

BLAST of Lag0004243 vs. NCBI nr
Match: XP_022157447.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 499.6 bits (1285), Expect = 2.5e-137
Identity = 243/284 (85.56%), Postives = 263/284 (92.61%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           M +L+LSILLC ML GPSLS ATSP+NIFILAGQSNMAGRGGVE D  G LVWDGYVPPE
Sbjct: 1   MVILRLSILLCMMLCGPSLSRATSPKNIFILAGQSNMAGRGGVENDRPGHLVWDGYVPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           AQP+PSILRL+PERQWE+A EPVH+GIDINKTVGVGPA+AFA  LQAK G+KVG VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGPAIAFARQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+ DTA+R
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTANR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TIDSLKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS YG
Sbjct: 241 TIDSLKLVNTTTVEGFNLDRGHFNIKTEIALGKWLADTYLSQYG 284

BLAST of Lag0004243 vs. NCBI nr
Match: XP_022158585.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 498.4 bits (1282), Expect = 5.5e-137
Identity = 240/284 (84.51%), Postives = 266/284 (93.66%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSILLC +L GPSLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPE
Sbjct: 1   MALLRLSILLCMILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           AQP+PSILRL+PERQWE+A EPVH+GIDINKTVGVG A+AFAH LQAK G+KVG VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+ DTASR
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPII++KIA YDTF++HDTHDLP+VRAAEDAVQREL ++V
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIIVIKIAGYDTFIEHDTHDLPTVRAAEDAVQRELLNIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TID+LKLVNT T EGFN D GH+N+KTEIALGKWLADTYLSHYG
Sbjct: 241 TIDALKLVNTITGEGFNLDRGHYNVKTEIALGKWLADTYLSHYG 284

BLAST of Lag0004243 vs. NCBI nr
Match: XP_023002178.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 492.3 bits (1266), Expect = 4.0e-135
Identity = 237/284 (83.45%), Postives = 259/284 (91.20%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MA L+L+I +C MLF PSL GATSP+NIFILAGQSNMAGRGGVEKDH G LVWDGY+PPE
Sbjct: 1   MAWLRLAIWVCMMLFYPSLLGATSPKNIFILAGQSNMAGRGGVEKDHMGQLVWDGYIPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           A  +P+ILR SPE QWEIALEPVHDGIDINKTVGVG A+ FA  LQAK+ ++ GVVGLVP
Sbjct: 61  AYSDPTILRFSPEGQWEIALEPVHDGIDINKTVGVGSAIVFARQLQAKSKSEAGVVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIE+WIKNP+NP ATFYQNFIERIKAS++ GGVVRAL WLQGESDAAASDTA R
Sbjct: 121 CARGGTLIEEWIKNPSNPEATFYQNFIERIKASEKDGGVVRALIWLQGESDAAASDTAHR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YK+NLKKFF DIRNDIKPRFLPIILVKIAVYDT++KHDTHDLP+VRAAEDAVQ+ELPD+V
Sbjct: 181 YKDNLKKFFMDIRNDIKPRFLPIILVKIAVYDTYMKHDTHDLPAVRAAEDAVQKELPDIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TIDSL LVNT THEGFNQDHGHFN KT+IALGKWLADTYLSHYG
Sbjct: 241 TIDSLNLVNTVTHEGFNQDHGHFNTKTQIALGKWLADTYLSHYG 284

BLAST of Lag0004243 vs. NCBI nr
Match: XP_022158593.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-133
Identity = 232/272 (85.29%), Postives = 255/272 (93.75%), Query Frame = 0

Query: 13  MLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPEAQPNPSILRLSP 72
           +L GPSLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPEAQP+PSILRL+P
Sbjct: 2   ILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPEAQPDPSILRLNP 61

Query: 73  ERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWI 132
           ERQWE+A EPVH+GIDINKTVGVG A+AFAH LQAK G+KVG VGLVPCARGGTLIEQW+
Sbjct: 62  ERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVPCARGGTLIEQWV 121

Query: 133 KNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDI 192
           KNP+N +ATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+ DTASRYKNNLKKFFTDI
Sbjct: 122 KNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASRYKNNLKKFFTDI 181

Query: 193 RNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRT 252
           RNDIKPRFLPII+VKIA YDTF++HDTHDLP+VRAAEDAVQRELP++VTID+LKLVNT T
Sbjct: 182 RNDIKPRFLPIIVVKIAGYDTFMEHDTHDLPAVRAAEDAVQRELPNIVTIDALKLVNTVT 241

Query: 253 HEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
            EGFN D GH+N KTEIALGKWLADTYLSHYG
Sbjct: 242 GEGFNSDRGHYNFKTEIALGKWLADTYLSHYG 273

BLAST of Lag0004243 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.4e-45
Identity = 104/268 (38.81%), Postives = 148/268 (55.22%), Query Frame = 0

Query: 17  PSLSGATSPQNIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPNPSILRLSPERQ 76
           P +     P  IFIL+GQSNMAGRGGV KD H+   VWD  +PPE  PN SILRLS + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWIKNP 136
           WE A EP+H  ID  K  GVGP MAFA+ ++ +  T   V+GLVPCA GGT I++W +  
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 137 NNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRND 196
           +      Y+  ++R + S + GG ++A+ W QGESD      A  Y NN+ +   ++R+D
Sbjct: 133 H-----LYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHD 192

Query: 197 IKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEG 256
           +    LPII V IA    ++          +  E  +  +L +VV +D+  L        
Sbjct: 193 LNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGL-------P 252

Query: 257 FNQDHGHFNIKTEIALGKWLADTYLSHY 284
              D+ H   + ++ LG  LA  YLS++
Sbjct: 253 LKSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Lag0004243 vs. ExPASy TrEMBL
Match: A0A6J1BQ38 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111004778 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 5.8e-140
Identity = 245/284 (86.27%), Postives = 267/284 (94.01%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSI+LC MLFGPSLSGATSP+NIFILAGQSNMAGRGGVEK+ +G L WDGYVPPE
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           +QP+PSILRL+PERQWE+A EPVH GIDI KTVGVGPA+AFAH LQAK G+KVG VGLVP
Sbjct: 61  SQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+NPNATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+SDTA R
Sbjct: 121 CARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAER 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPR LPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TID+LKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS+YG
Sbjct: 241 TIDALKLVNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYG 284

BLAST of Lag0004243 vs. ExPASy TrEMBL
Match: A0A6J1DUH7 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111024143 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 1.2e-137
Identity = 243/284 (85.56%), Postives = 263/284 (92.61%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           M +L+LSILLC ML GPSLS ATSP+NIFILAGQSNMAGRGGVE D  G LVWDGYVPPE
Sbjct: 1   MVILRLSILLCMMLCGPSLSRATSPKNIFILAGQSNMAGRGGVENDRPGHLVWDGYVPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           AQP+PSILRL+PERQWE+A EPVH+GIDINKTVGVGPA+AFA  LQAK G+KVG VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGPAIAFARQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+ DTA+R
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTANR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTF+KHDTHDLP+VRAAEDAVQRELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TIDSLKLVNT T EGFN D GHFNIKTEIALGKWLADTYLS YG
Sbjct: 241 TIDSLKLVNTTTVEGFNLDRGHFNIKTEIALGKWLADTYLSQYG 284

BLAST of Lag0004243 vs. ExPASy TrEMBL
Match: A0A6J1DW87 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111025037 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.7e-137
Identity = 240/284 (84.51%), Postives = 266/284 (93.66%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MALL+LSILLC +L GPSLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPE
Sbjct: 1   MALLRLSILLCMILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           AQP+PSILRL+PERQWE+A EPVH+GIDINKTVGVG A+AFAH LQAK G+KVG VGLVP
Sbjct: 61  AQPDPSILRLNPERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIEQW+KNP+N +ATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+ DTASR
Sbjct: 121 CARGGTLIEQWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YKNNLKKFFTDIRNDIKPRFLPII++KIA YDTF++HDTHDLP+VRAAEDAVQREL ++V
Sbjct: 181 YKNNLKKFFTDIRNDIKPRFLPIIVIKIAGYDTFIEHDTHDLPTVRAAEDAVQRELLNIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TID+LKLVNT T EGFN D GH+N+KTEIALGKWLADTYLSHYG
Sbjct: 241 TIDALKLVNTITGEGFNLDRGHYNVKTEIALGKWLADTYLSHYG 284

BLAST of Lag0004243 vs. ExPASy TrEMBL
Match: A0A6J1KST3 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496117 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.9e-135
Identity = 237/284 (83.45%), Postives = 259/284 (91.20%), Query Frame = 0

Query: 1   MALLKLSILLCTMLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPE 60
           MA L+L+I +C MLF PSL GATSP+NIFILAGQSNMAGRGGVEKDH G LVWDGY+PPE
Sbjct: 1   MAWLRLAIWVCMMLFYPSLLGATSPKNIFILAGQSNMAGRGGVEKDHMGQLVWDGYIPPE 60

Query: 61  AQPNPSILRLSPERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVP 120
           A  +P+ILR SPE QWEIALEPVHDGIDINKTVGVG A+ FA  LQAK+ ++ GVVGLVP
Sbjct: 61  AYSDPTILRFSPEGQWEIALEPVHDGIDINKTVGVGSAIVFARQLQAKSKSEAGVVGLVP 120

Query: 121 CARGGTLIEQWIKNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASR 180
           CARGGTLIE+WIKNP+NP ATFYQNFIERIKAS++ GGVVRAL WLQGESDAAASDTA R
Sbjct: 121 CARGGTLIEEWIKNPSNPEATFYQNFIERIKASEKDGGVVRALIWLQGESDAAASDTAHR 180

Query: 181 YKNNLKKFFTDIRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVV 240
           YK+NLKKFF DIRNDIKPRFLPIILVKIAVYDT++KHDTHDLP+VRAAEDAVQ+ELPD+V
Sbjct: 181 YKDNLKKFFMDIRNDIKPRFLPIILVKIAVYDTYMKHDTHDLPAVRAAEDAVQKELPDIV 240

Query: 241 TIDSLKLVNTRTHEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
           TIDSL LVNT THEGFNQDHGHFN KT+IALGKWLADTYLSHYG
Sbjct: 241 TIDSLNLVNTVTHEGFNQDHGHFNTKTQIALGKWLADTYLSHYG 284

BLAST of Lag0004243 vs. ExPASy TrEMBL
Match: A0A6J1DWJ1 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111025047 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 8.1e-134
Identity = 232/272 (85.29%), Postives = 255/272 (93.75%), Query Frame = 0

Query: 13  MLFGPSLSGATSPQNIFILAGQSNMAGRGGVEKDHSGVLVWDGYVPPEAQPNPSILRLSP 72
           +L GPSLSGATSP+NIFILAGQSNMAGRGGVEKD SG LVWDGYVPPEAQP+PSILRL+P
Sbjct: 2   ILCGPSLSGATSPKNIFILAGQSNMAGRGGVEKDRSGNLVWDGYVPPEAQPDPSILRLNP 61

Query: 73  ERQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWI 132
           ERQWE+A EPVH+GIDINKTVGVG A+AFAH LQAK G+KVG VGLVPCARGGTLIEQW+
Sbjct: 62  ERQWEVAREPVHEGIDINKTVGVGSAIAFAHQLQAKGGSKVGSVGLVPCARGGTLIEQWV 121

Query: 133 KNPNNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDI 192
           KNP+N +ATFY+NFIERI+ASD +GGVVRALFWLQGESDAA+ DTASRYKNNLKKFFTDI
Sbjct: 122 KNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTASRYKNNLKKFFTDI 181

Query: 193 RNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRT 252
           RNDIKPRFLPII+VKIA YDTF++HDTHDLP+VRAAEDAVQRELP++VTID+LKLVNT T
Sbjct: 182 RNDIKPRFLPIIVVKIAGYDTFMEHDTHDLPAVRAAEDAVQRELPNIVTIDALKLVNTVT 241

Query: 253 HEGFNQDHGHFNIKTEIALGKWLADTYLSHYG 285
            EGFN D GH+N KTEIALGKWLADTYLSHYG
Sbjct: 242 GEGFNSDRGHYNFKTEIALGKWLADTYLSHYG 273

BLAST of Lag0004243 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 185.3 bits (469), Expect = 9.7e-47
Identity = 104/268 (38.81%), Postives = 148/268 (55.22%), Query Frame = 0

Query: 17  PSLSGATSPQNIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPNPSILRLSPERQ 76
           P +     P  IFIL+GQSNMAGRGGV KD H+   VWD  +PPE  PN SILRLS + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWIKNP 136
           WE A EP+H  ID  K  GVGP MAFA+ ++ +  T   V+GLVPCA GGT I++W +  
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 137 NNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRND 196
           +      Y+  ++R + S + GG ++A+ W QGESD      A  Y NN+ +   ++R+D
Sbjct: 133 H-----LYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHD 192

Query: 197 IKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEG 256
           +    LPII V IA    ++          +  E  +  +L +VV +D+  L        
Sbjct: 193 LNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGL-------P 252

Query: 257 FNQDHGHFNIKTEIALGKWLADTYLSHY 284
              D+ H   + ++ LG  LA  YLS++
Sbjct: 253 LKSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Lag0004243 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 185.3 bits (469), Expect = 9.7e-47
Identity = 104/268 (38.81%), Postives = 148/268 (55.22%), Query Frame = 0

Query: 17  PSLSGATSPQNIFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPNPSILRLSPERQ 76
           P +     P  IFIL+GQSNMAGRGGV KD H+   VWD  +PPE  PN SILRLS + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 77  WEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWIKNP 136
           WE A EP+H  ID  K  GVGP MAFA+ ++ +  T   V+GLVPCA GGT I++W +  
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 137 NNPNATFYQNFIERIKASDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTDIRND 196
           +      Y+  ++R + S + GG ++A+ W QGESD      A  Y NN+ +   ++R+D
Sbjct: 133 H-----LYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHD 192

Query: 197 IKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTRTHEG 256
           +    LPII V IA    ++          +  E  +  +L +VV +D+  L        
Sbjct: 193 LNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGL-------P 252

Query: 257 FNQDHGHFNIKTEIALGKWLADTYLSHY 284
              D+ H   + ++ LG  LA  YLS++
Sbjct: 253 LKSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Lag0004243 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 178.3 bits (451), Expect = 1.2e-44
Identity = 112/270 (41.48%), Postives = 152/270 (56.30%), Query Frame = 0

Query: 17  PSLSGATSPQN--IFILAGQSNMAGRGGVEKD-HSGVLVWDGYVPPEAQPNPSILRLSPE 76
           P L   T  +N  IFILAGQSNMAGRGGV  D  +   VWDG +PPE + NPSILRL+ +
Sbjct: 18  PHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSK 77

Query: 77  RQWEIALEPVHDGIDINKTVGVGPAMAFAHHLQAKAGTKVGVVGLVPCARGGTLIEQWIK 136
            +W+ A EP+H  IDINKT GVGP M FA+    +   + G VGLVPC+ GGT + QW K
Sbjct: 78  LEWKEAKEPLHVDIDINKTNGVGPGMPFAN----RVVNRFGQVGLVPCSIGGTKLSQWQK 137

Query: 137 NPNNPNATFYQNFIERIKA--SDEKGGVVRALFWLQGESDAAASDTASRYKNNLKKFFTD 196
                    Y+  ++R KA  +   GG  RA+ W QGESD      AS YK  L KFF+D
Sbjct: 138 -----GEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSD 197

Query: 197 IRNDIKPRFLPIILVKIAVYDTFLKHDTHDLPSVRAAEDAVQRELPDVVTIDSLKLVNTR 256
           +RND++   LPII V +A            L +VR A+  ++ +L +V  +D+  L    
Sbjct: 198 LRNDLQHPNLPIIQVALAT------GAGPYLDAVRKAQ--LKTDLENVYCVDARGL---- 257

Query: 257 THEGFNQDHGHFNIKTEIALGKWLADTYLS 282
                  D  H    +++ LG  +A+++L+
Sbjct: 258 ---PLEPDGLHLTTSSQVQLGHMIAESFLA 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131651.11.2e-13986.27probable carbohydrate esterase At4g34215 [Momordica charantia][more]
XP_022157447.12.5e-13785.56probable carbohydrate esterase At4g34215 [Momordica charantia][more]
XP_022158585.15.5e-13784.51probable carbohydrate esterase At4g34215 [Momordica charantia][more]
XP_023002178.14.0e-13583.45probable carbohydrate esterase At4g34215 [Cucurbita maxima][more]
XP_022158593.11.7e-13385.29probable carbohydrate esterase At4g34215 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q8L9J91.4e-4538.81Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ385.8e-14086.27probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1DUH71.2e-13785.56probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1DW872.7e-13784.51probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1KST31.9e-13583.45probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1DWJ18.1e-13485.29probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT4G34215.19.7e-4738.81Domain of unknown function (DUF303) [more]
AT4G34215.29.7e-4738.81Domain of unknown function (DUF303) [more]
AT3G53010.11.2e-4441.48Domain of unknown function (DUF303) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005181Sialate O-acetylesterase domainPFAMPF03629SASAcoord: 26..280
e-value: 1.4E-65
score: 221.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 289..387
e-value: 1.4E-8
score: 34.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 24..284
e-value: 9.8E-56
score: 191.3
NoneNo IPR availablePANTHERPTHR31988:SF19BNACNNG62850D PROTEINcoord: 23..283
NoneNo IPR availablePANTHERPTHR31988ESTERASE, PUTATIVE (DUF303)-RELATEDcoord: 23..283
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 27..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004243.1Lag0004243.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity