Lag0003222 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003222 vs. NCBI nr
Match: XP_038897397.1 (uncharacterized protein LOC120085485 isoform X4 [Benincasa hispida] >XP_038897399.1 uncharacterized protein LOC120085485 isoform X4 [Benincasa hispida])

HSP 1 Score: 2407.5 bits (6238), Expect = 0.0e+00
Identity = 1293/1500 (86.20%), Postives = 1365/1500 (91.00%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS+IELSG+KMAV+D  GFNWLHDLMDWGKSSLKVVLTYWRR
Sbjct: 486  MTCVRLLEIIPIVFERFNPSLIELSGTKMAVEDACGFNWLHDLMDWGKSSLKVVLTYWRR 545

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDAVVMDELTEKVAHLTILLSKNEKHNVAE 120
            A+ISLL+FIKGSC LS TSTIR I +L+S DDA  MDELTEKVAHLTILLSKNEK+N+ +
Sbjct: 546  AIISLLNFIKGSCCLSTTSTIRDIEHLMSLDDA-AMDELTEKVAHLTILLSKNEKYNIVK 605

Query: 121  TNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDESK 180
            TNLGSNA  LEDFPSGR LSTSK ES G EDI+V TL  R EAKKE+I ELIVLSDDESK
Sbjct: 606  TNLGSNALVLEDFPSGRKLSTSKLESSGAEDIEVPTLVKRSEAKKENIGELIVLSDDESK 665

Query: 181  EYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEINN 240
             +I PT  F SESDVGQ IL   LAP  +E+ TRADFGK+ ILV E  KYV + DQEIN+
Sbjct: 666  PFISPTRAFPSESDVGQCILDDKLAPG-DESDTRADFGKSNILVIEPSKYVADRDQEIND 725

Query: 241  KCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKALS 300
            KCSST+ALK+ ASGDS+ RPATSSVLRSKDVD K +E+DSEC+L+ HV LNDRIDLK LS
Sbjct: 726  KCSSTLALKEHASGDSKVRPATSSVLRSKDVDVKHKEIDSECILSKHVPLNDRIDLKVLS 785

Query: 301  NKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVPK 360
            NKATGSKSKNQSCETAVSV GYAVLKQVVSDAADDPLEIELNS RNQKTNISKPI TVPK
Sbjct: 786  NKATGSKSKNQSCETAVSVPGYAVLKQVVSDAADDPLEIELNSVRNQKTNISKPIITVPK 845

Query: 361  RRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQT 420
            RRVIQLKTPVE+RAV+ HRHMIGAKRFKPPRL+DWYRSILELDYFAMIG T V+EDKSQ 
Sbjct: 846  RRVIQLKTPVESRAVHLHRHMIGAKRFKPPRLEDWYRSILELDYFAMIGLTSVTEDKSQA 905

Query: 421  VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVD 480
            V+HLKEVPVCFQSSEQYVEIFRPLILEEFKAQLR+SFVEMSSWDEMYLG ISVLS+ERVD
Sbjct: 906  VKHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRNSFVEMSSWDEMYLGRISVLSIERVD 965

Query: 481  EFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNLL 540
            EFHLVRFAYDDNNSVASK+FAENDL+LLTKELPQKS QGAHMVGKVDRRERDNKRKMNLL
Sbjct: 966  EFHLVRFAYDDNNSVASKNFAENDLILLTKELPQKSPQGAHMVGKVDRRERDNKRKMNLL 1025

Query: 541  IIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPE 600
            I+RFY LNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPI+P IL P+
Sbjct: 1026 IVRFYFLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPIVPTILNPK 1085

Query: 601  ANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 660
            ++SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG
Sbjct: 1086 SSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 1145

Query: 661  TGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQNA 720
            TGKTRTILAIVSALLASASQRTN  A+SLNRSLKQDNVLHADSRPQISQ VAIARAWQNA
Sbjct: 1146 TGKTRTILAIVSALLASASQRTNLAASSLNRSLKQDNVLHADSRPQISQTVAIARAWQNA 1205

Query: 721  ALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 780
            ALARQLNEDKQRN K IDCTMKRR+LICAQSNAAVDELVSRIS+LGLYDSDGKMYKPYLV
Sbjct: 1206 ALARQLNEDKQRNLKSIDCTMKRRILICAQSNAAVDELVSRISSLGLYDSDGKMYKPYLV 1265

Query: 781  RVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKY 840
            RVGNAKTVHPNSLPF+ID+LVDQQLAEERM+S+DVKNDL TNSS ELRSNLEKLVDRI+Y
Sbjct: 1266 RVGNAKTVHPNSLPFYIDSLVDQQLAEERMSSSDVKNDLGTNSSTELRSNLEKLVDRIRY 1325

Query: 841  YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAFE 900
             EVKCANLRDENP+ KSSV+ + G DEKEMSLKELE KLRKLYEQKKQIYKDISIAQAFE
Sbjct: 1326 CEVKCANLRDENPEPKSSVENWMGEDEKEMSLKELESKLRKLYEQKKQIYKDISIAQAFE 1385

Query: 901  KQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDA 960
            K+TNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFDA
Sbjct: 1386 KKTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFDA 1445

Query: 961  VVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1020
            VVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ
Sbjct: 1446 VVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1505

Query: 1021 RAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDIV 1080
            RAGHPVVMLTRQYRMHPEICHFP+QHFYDGKLLNGDGMSGKIAPFHETKGLGPY F+DIV
Sbjct: 1506 RAGHPVVMLTRQYRMHPEICHFPAQHFYDGKLLNGDGMSGKIAPFHETKGLGPYNFFDIV 1565

Query: 1081 DGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRSC 1140
            DGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEFSRVRIGIITPYKCQLSLLRS 
Sbjct: 1566 DGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFSRVRIGIITPYKCQLSLLRSR 1625

Query: 1141 FSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRMN 1200
            FS SFGASLIVDMEFNTVDGFQGREVDILILSTVRA D SSTS  NSSGIGFVADARRMN
Sbjct: 1626 FSHSFGASLIVDMEFNTVDGFQGREVDILILSTVRAVDPSSTSRKNSSGIGFVADARRMN 1685

Query: 1201 VALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQQ 1260
            VALTRAKLSLWVLGNSRTLQVNPDWGAL+KDAKERNLVVSVK PYDS++KTA  R    Q
Sbjct: 1686 VALTRAKLSLWVLGNSRTLQVNPDWGALLKDAKERNLVVSVKKPYDSMFKTATPRYPCPQ 1745

Query: 1261 TMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK-D 1320
            TM NNSRN KH     A RHAKRS KET E EGKDIL  TQCTKTNDVDSS+Y A VK D
Sbjct: 1746 TMTNNSRNPKHTDNVRARRHAKRSGKETFECEGKDIL--TQCTKTNDVDSSRYKAPVKED 1805

Query: 1321 ALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSNS 1380
            A+PLVAGSI+RS K AK+AV +EHGA+FGSKSG S+EKK N GNIS+GKRKVD  KSSN 
Sbjct: 1806 AIPLVAGSIDRSSKAAKSAVRMEHGADFGSKSGKSTEKKFNMGNISQGKRKVDREKSSNF 1865

Query: 1381 DYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRSD 1440
            D++ERG+VDNHALQ S+TSKRLKESP        QE S P+IEGSSKEEHN+  A+SRSD
Sbjct: 1866 DFSERGMVDNHALQKSKTSKRLKESP--------QEASDPLIEGSSKEEHNDGVAISRSD 1925

Query: 1441 TGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRKG 1492
              KELIVKRKQQREAVDAILFSSLIPSKKSEMSMK +SDKKP   SNVRG MKPPKGRKG
Sbjct: 1926 NEKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKHISDKKPHSLSNVRGSMKPPKGRKG 1973

BLAST of Lag0003222 vs. NCBI nr
Match: XP_038897400.1 (uncharacterized protein LOC120085485 isoform X5 [Benincasa hispida])

HSP 1 Score: 2407.5 bits (6238), Expect = 0.0e+00
Identity = 1293/1500 (86.20%), Postives = 1365/1500 (91.00%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS+IELSG+KMAV+D  GFNWLHDLMDWGKSSLKVVLTYWRR
Sbjct: 433  MTCVRLLEIIPIVFERFNPSLIELSGTKMAVEDACGFNWLHDLMDWGKSSLKVVLTYWRR 492

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDAVVMDELTEKVAHLTILLSKNEKHNVAE 120
            A+ISLL+FIKGSC LS TSTIR I +L+S DDA  MDELTEKVAHLTILLSKNEK+N+ +
Sbjct: 493  AIISLLNFIKGSCCLSTTSTIRDIEHLMSLDDA-AMDELTEKVAHLTILLSKNEKYNIVK 552

Query: 121  TNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDESK 180
            TNLGSNA  LEDFPSGR LSTSK ES G EDI+V TL  R EAKKE+I ELIVLSDDESK
Sbjct: 553  TNLGSNALVLEDFPSGRKLSTSKLESSGAEDIEVPTLVKRSEAKKENIGELIVLSDDESK 612

Query: 181  EYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEINN 240
             +I PT  F SESDVGQ IL   LAP  +E+ TRADFGK+ ILV E  KYV + DQEIN+
Sbjct: 613  PFISPTRAFPSESDVGQCILDDKLAPG-DESDTRADFGKSNILVIEPSKYVADRDQEIND 672

Query: 241  KCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKALS 300
            KCSST+ALK+ ASGDS+ RPATSSVLRSKDVD K +E+DSEC+L+ HV LNDRIDLK LS
Sbjct: 673  KCSSTLALKEHASGDSKVRPATSSVLRSKDVDVKHKEIDSECILSKHVPLNDRIDLKVLS 732

Query: 301  NKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVPK 360
            NKATGSKSKNQSCETAVSV GYAVLKQVVSDAADDPLEIELNS RNQKTNISKPI TVPK
Sbjct: 733  NKATGSKSKNQSCETAVSVPGYAVLKQVVSDAADDPLEIELNSVRNQKTNISKPIITVPK 792

Query: 361  RRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQT 420
            RRVIQLKTPVE+RAV+ HRHMIGAKRFKPPRL+DWYRSILELDYFAMIG T V+EDKSQ 
Sbjct: 793  RRVIQLKTPVESRAVHLHRHMIGAKRFKPPRLEDWYRSILELDYFAMIGLTSVTEDKSQA 852

Query: 421  VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVD 480
            V+HLKEVPVCFQSSEQYVEIFRPLILEEFKAQLR+SFVEMSSWDEMYLG ISVLS+ERVD
Sbjct: 853  VKHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRNSFVEMSSWDEMYLGRISVLSIERVD 912

Query: 481  EFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNLL 540
            EFHLVRFAYDDNNSVASK+FAENDL+LLTKELPQKS QGAHMVGKVDRRERDNKRKMNLL
Sbjct: 913  EFHLVRFAYDDNNSVASKNFAENDLILLTKELPQKSPQGAHMVGKVDRRERDNKRKMNLL 972

Query: 541  IIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPE 600
            I+RFY LNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPI+P IL P+
Sbjct: 973  IVRFYFLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPIVPTILNPK 1032

Query: 601  ANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 660
            ++SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG
Sbjct: 1033 SSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 1092

Query: 661  TGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQNA 720
            TGKTRTILAIVSALLASASQRTN  A+SLNRSLKQDNVLHADSRPQISQ VAIARAWQNA
Sbjct: 1093 TGKTRTILAIVSALLASASQRTNLAASSLNRSLKQDNVLHADSRPQISQTVAIARAWQNA 1152

Query: 721  ALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 780
            ALARQLNEDKQRN K IDCTMKRR+LICAQSNAAVDELVSRIS+LGLYDSDGKMYKPYLV
Sbjct: 1153 ALARQLNEDKQRNLKSIDCTMKRRILICAQSNAAVDELVSRISSLGLYDSDGKMYKPYLV 1212

Query: 781  RVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKY 840
            RVGNAKTVHPNSLPF+ID+LVDQQLAEERM+S+DVKNDL TNSS ELRSNLEKLVDRI+Y
Sbjct: 1213 RVGNAKTVHPNSLPFYIDSLVDQQLAEERMSSSDVKNDLGTNSSTELRSNLEKLVDRIRY 1272

Query: 841  YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAFE 900
             EVKCANLRDENP+ KSSV+ + G DEKEMSLKELE KLRKLYEQKKQIYKDISIAQAFE
Sbjct: 1273 CEVKCANLRDENPEPKSSVENWMGEDEKEMSLKELESKLRKLYEQKKQIYKDISIAQAFE 1332

Query: 901  KQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDA 960
            K+TNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFDA
Sbjct: 1333 KKTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFDA 1392

Query: 961  VVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1020
            VVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ
Sbjct: 1393 VVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1452

Query: 1021 RAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDIV 1080
            RAGHPVVMLTRQYRMHPEICHFP+QHFYDGKLLNGDGMSGKIAPFHETKGLGPY F+DIV
Sbjct: 1453 RAGHPVVMLTRQYRMHPEICHFPAQHFYDGKLLNGDGMSGKIAPFHETKGLGPYNFFDIV 1512

Query: 1081 DGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRSC 1140
            DGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEFSRVRIGIITPYKCQLSLLRS 
Sbjct: 1513 DGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFSRVRIGIITPYKCQLSLLRSR 1572

Query: 1141 FSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRMN 1200
            FS SFGASLIVDMEFNTVDGFQGREVDILILSTVRA D SSTS  NSSGIGFVADARRMN
Sbjct: 1573 FSHSFGASLIVDMEFNTVDGFQGREVDILILSTVRAVDPSSTSRKNSSGIGFVADARRMN 1632

Query: 1201 VALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQQ 1260
            VALTRAKLSLWVLGNSRTLQVNPDWGAL+KDAKERNLVVSVK PYDS++KTA  R    Q
Sbjct: 1633 VALTRAKLSLWVLGNSRTLQVNPDWGALLKDAKERNLVVSVKKPYDSMFKTATPRYPCPQ 1692

Query: 1261 TMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK-D 1320
            TM NNSRN KH     A RHAKRS KET E EGKDIL  TQCTKTNDVDSS+Y A VK D
Sbjct: 1693 TMTNNSRNPKHTDNVRARRHAKRSGKETFECEGKDIL--TQCTKTNDVDSSRYKAPVKED 1752

Query: 1321 ALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSNS 1380
            A+PLVAGSI+RS K AK+AV +EHGA+FGSKSG S+EKK N GNIS+GKRKVD  KSSN 
Sbjct: 1753 AIPLVAGSIDRSSKAAKSAVRMEHGADFGSKSGKSTEKKFNMGNISQGKRKVDREKSSNF 1812

Query: 1381 DYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRSD 1440
            D++ERG+VDNHALQ S+TSKRLKESP        QE S P+IEGSSKEEHN+  A+SRSD
Sbjct: 1813 DFSERGMVDNHALQKSKTSKRLKESP--------QEASDPLIEGSSKEEHNDGVAISRSD 1872

Query: 1441 TGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRKG 1492
              KELIVKRKQQREAVDAILFSSLIPSKKSEMSMK +SDKKP   SNVRG MKPPKGRKG
Sbjct: 1873 NEKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKHISDKKPHSLSNVRGSMKPPKGRKG 1920

BLAST of Lag0003222 vs. NCBI nr
Match: XP_038897396.1 (uncharacterized protein LOC120085485 isoform X3 [Benincasa hispida])

HSP 1 Score: 2407.5 bits (6238), Expect = 0.0e+00
Identity = 1293/1500 (86.20%), Postives = 1365/1500 (91.00%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS+IELSG+KMAV+D  GFNWLHDLMDWGKSSLKVVLTYWRR
Sbjct: 598  MTCVRLLEIIPIVFERFNPSLIELSGTKMAVEDACGFNWLHDLMDWGKSSLKVVLTYWRR 657

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDAVVMDELTEKVAHLTILLSKNEKHNVAE 120
            A+ISLL+FIKGSC LS TSTIR I +L+S DDA  MDELTEKVAHLTILLSKNEK+N+ +
Sbjct: 658  AIISLLNFIKGSCCLSTTSTIRDIEHLMSLDDA-AMDELTEKVAHLTILLSKNEKYNIVK 717

Query: 121  TNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDESK 180
            TNLGSNA  LEDFPSGR LSTSK ES G EDI+V TL  R EAKKE+I ELIVLSDDESK
Sbjct: 718  TNLGSNALVLEDFPSGRKLSTSKLESSGAEDIEVPTLVKRSEAKKENIGELIVLSDDESK 777

Query: 181  EYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEINN 240
             +I PT  F SESDVGQ IL   LAP  +E+ TRADFGK+ ILV E  KYV + DQEIN+
Sbjct: 778  PFISPTRAFPSESDVGQCILDDKLAPG-DESDTRADFGKSNILVIEPSKYVADRDQEIND 837

Query: 241  KCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKALS 300
            KCSST+ALK+ ASGDS+ RPATSSVLRSKDVD K +E+DSEC+L+ HV LNDRIDLK LS
Sbjct: 838  KCSSTLALKEHASGDSKVRPATSSVLRSKDVDVKHKEIDSECILSKHVPLNDRIDLKVLS 897

Query: 301  NKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVPK 360
            NKATGSKSKNQSCETAVSV GYAVLKQVVSDAADDPLEIELNS RNQKTNISKPI TVPK
Sbjct: 898  NKATGSKSKNQSCETAVSVPGYAVLKQVVSDAADDPLEIELNSVRNQKTNISKPIITVPK 957

Query: 361  RRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQT 420
            RRVIQLKTPVE+RAV+ HRHMIGAKRFKPPRL+DWYRSILELDYFAMIG T V+EDKSQ 
Sbjct: 958  RRVIQLKTPVESRAVHLHRHMIGAKRFKPPRLEDWYRSILELDYFAMIGLTSVTEDKSQA 1017

Query: 421  VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVD 480
            V+HLKEVPVCFQSSEQYVEIFRPLILEEFKAQLR+SFVEMSSWDEMYLG ISVLS+ERVD
Sbjct: 1018 VKHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRNSFVEMSSWDEMYLGRISVLSIERVD 1077

Query: 481  EFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNLL 540
            EFHLVRFAYDDNNSVASK+FAENDL+LLTKELPQKS QGAHMVGKVDRRERDNKRKMNLL
Sbjct: 1078 EFHLVRFAYDDNNSVASKNFAENDLILLTKELPQKSPQGAHMVGKVDRRERDNKRKMNLL 1137

Query: 541  IIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPE 600
            I+RFY LNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPI+P IL P+
Sbjct: 1138 IVRFYFLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPIVPTILNPK 1197

Query: 601  ANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 660
            ++SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG
Sbjct: 1198 SSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 1257

Query: 661  TGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQNA 720
            TGKTRTILAIVSALLASASQRTN  A+SLNRSLKQDNVLHADSRPQISQ VAIARAWQNA
Sbjct: 1258 TGKTRTILAIVSALLASASQRTNLAASSLNRSLKQDNVLHADSRPQISQTVAIARAWQNA 1317

Query: 721  ALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 780
            ALARQLNEDKQRN K IDCTMKRR+LICAQSNAAVDELVSRIS+LGLYDSDGKMYKPYLV
Sbjct: 1318 ALARQLNEDKQRNLKSIDCTMKRRILICAQSNAAVDELVSRISSLGLYDSDGKMYKPYLV 1377

Query: 781  RVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKY 840
            RVGNAKTVHPNSLPF+ID+LVDQQLAEERM+S+DVKNDL TNSS ELRSNLEKLVDRI+Y
Sbjct: 1378 RVGNAKTVHPNSLPFYIDSLVDQQLAEERMSSSDVKNDLGTNSSTELRSNLEKLVDRIRY 1437

Query: 841  YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAFE 900
             EVKCANLRDENP+ KSSV+ + G DEKEMSLKELE KLRKLYEQKKQIYKDISIAQAFE
Sbjct: 1438 CEVKCANLRDENPEPKSSVENWMGEDEKEMSLKELESKLRKLYEQKKQIYKDISIAQAFE 1497

Query: 901  KQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDA 960
            K+TNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFDA
Sbjct: 1498 KKTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFDA 1557

Query: 961  VVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1020
            VVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ
Sbjct: 1558 VVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1617

Query: 1021 RAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDIV 1080
            RAGHPVVMLTRQYRMHPEICHFP+QHFYDGKLLNGDGMSGKIAPFHETKGLGPY F+DIV
Sbjct: 1618 RAGHPVVMLTRQYRMHPEICHFPAQHFYDGKLLNGDGMSGKIAPFHETKGLGPYNFFDIV 1677

Query: 1081 DGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRSC 1140
            DGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEFSRVRIGIITPYKCQLSLLRS 
Sbjct: 1678 DGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFSRVRIGIITPYKCQLSLLRSR 1737

Query: 1141 FSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRMN 1200
            FS SFGASLIVDMEFNTVDGFQGREVDILILSTVRA D SSTS  NSSGIGFVADARRMN
Sbjct: 1738 FSHSFGASLIVDMEFNTVDGFQGREVDILILSTVRAVDPSSTSRKNSSGIGFVADARRMN 1797

Query: 1201 VALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQQ 1260
            VALTRAKLSLWVLGNSRTLQVNPDWGAL+KDAKERNLVVSVK PYDS++KTA  R    Q
Sbjct: 1798 VALTRAKLSLWVLGNSRTLQVNPDWGALLKDAKERNLVVSVKKPYDSMFKTATPRYPCPQ 1857

Query: 1261 TMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK-D 1320
            TM NNSRN KH     A RHAKRS KET E EGKDIL  TQCTKTNDVDSS+Y A VK D
Sbjct: 1858 TMTNNSRNPKHTDNVRARRHAKRSGKETFECEGKDIL--TQCTKTNDVDSSRYKAPVKED 1917

Query: 1321 ALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSNS 1380
            A+PLVAGSI+RS K AK+AV +EHGA+FGSKSG S+EKK N GNIS+GKRKVD  KSSN 
Sbjct: 1918 AIPLVAGSIDRSSKAAKSAVRMEHGADFGSKSGKSTEKKFNMGNISQGKRKVDREKSSNF 1977

Query: 1381 DYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRSD 1440
            D++ERG+VDNHALQ S+TSKRLKESP        QE S P+IEGSSKEEHN+  A+SRSD
Sbjct: 1978 DFSERGMVDNHALQKSKTSKRLKESP--------QEASDPLIEGSSKEEHNDGVAISRSD 2037

Query: 1441 TGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRKG 1492
              KELIVKRKQQREAVDAILFSSLIPSKKSEMSMK +SDKKP   SNVRG MKPPKGRKG
Sbjct: 2038 NEKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKHISDKKPHSLSNVRGSMKPPKGRKG 2085

BLAST of Lag0003222 vs. NCBI nr
Match: XP_038897394.1 (uncharacterized protein LOC120085485 isoform X1 [Benincasa hispida])

HSP 1 Score: 2407.5 bits (6238), Expect = 0.0e+00
Identity = 1293/1500 (86.20%), Postives = 1365/1500 (91.00%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS+IELSG+KMAV+D  GFNWLHDLMDWGKSSLKVVLTYWRR
Sbjct: 834  MTCVRLLEIIPIVFERFNPSLIELSGTKMAVEDACGFNWLHDLMDWGKSSLKVVLTYWRR 893

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDAVVMDELTEKVAHLTILLSKNEKHNVAE 120
            A+ISLL+FIKGSC LS TSTIR I +L+S DDA  MDELTEKVAHLTILLSKNEK+N+ +
Sbjct: 894  AIISLLNFIKGSCCLSTTSTIRDIEHLMSLDDA-AMDELTEKVAHLTILLSKNEKYNIVK 953

Query: 121  TNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDESK 180
            TNLGSNA  LEDFPSGR LSTSK ES G EDI+V TL  R EAKKE+I ELIVLSDDESK
Sbjct: 954  TNLGSNALVLEDFPSGRKLSTSKLESSGAEDIEVPTLVKRSEAKKENIGELIVLSDDESK 1013

Query: 181  EYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEINN 240
             +I PT  F SESDVGQ IL   LAP  +E+ TRADFGK+ ILV E  KYV + DQEIN+
Sbjct: 1014 PFISPTRAFPSESDVGQCILDDKLAPG-DESDTRADFGKSNILVIEPSKYVADRDQEIND 1073

Query: 241  KCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKALS 300
            KCSST+ALK+ ASGDS+ RPATSSVLRSKDVD K +E+DSEC+L+ HV LNDRIDLK LS
Sbjct: 1074 KCSSTLALKEHASGDSKVRPATSSVLRSKDVDVKHKEIDSECILSKHVPLNDRIDLKVLS 1133

Query: 301  NKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVPK 360
            NKATGSKSKNQSCETAVSV GYAVLKQVVSDAADDPLEIELNS RNQKTNISKPI TVPK
Sbjct: 1134 NKATGSKSKNQSCETAVSVPGYAVLKQVVSDAADDPLEIELNSVRNQKTNISKPIITVPK 1193

Query: 361  RRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQT 420
            RRVIQLKTPVE+RAV+ HRHMIGAKRFKPPRL+DWYRSILELDYFAMIG T V+EDKSQ 
Sbjct: 1194 RRVIQLKTPVESRAVHLHRHMIGAKRFKPPRLEDWYRSILELDYFAMIGLTSVTEDKSQA 1253

Query: 421  VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVD 480
            V+HLKEVPVCFQSSEQYVEIFRPLILEEFKAQLR+SFVEMSSWDEMYLG ISVLS+ERVD
Sbjct: 1254 VKHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRNSFVEMSSWDEMYLGRISVLSIERVD 1313

Query: 481  EFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNLL 540
            EFHLVRFAYDDNNSVASK+FAENDL+LLTKELPQKS QGAHMVGKVDRRERDNKRKMNLL
Sbjct: 1314 EFHLVRFAYDDNNSVASKNFAENDLILLTKELPQKSPQGAHMVGKVDRRERDNKRKMNLL 1373

Query: 541  IIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPE 600
            I+RFY LNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPI+P IL P+
Sbjct: 1374 IVRFYFLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPIVPTILNPK 1433

Query: 601  ANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 660
            ++SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG
Sbjct: 1434 SSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 1493

Query: 661  TGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQNA 720
            TGKTRTILAIVSALLASASQRTN  A+SLNRSLKQDNVLHADSRPQISQ VAIARAWQNA
Sbjct: 1494 TGKTRTILAIVSALLASASQRTNLAASSLNRSLKQDNVLHADSRPQISQTVAIARAWQNA 1553

Query: 721  ALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 780
            ALARQLNEDKQRN K IDCTMKRR+LICAQSNAAVDELVSRIS+LGLYDSDGKMYKPYLV
Sbjct: 1554 ALARQLNEDKQRNLKSIDCTMKRRILICAQSNAAVDELVSRISSLGLYDSDGKMYKPYLV 1613

Query: 781  RVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKY 840
            RVGNAKTVHPNSLPF+ID+LVDQQLAEERM+S+DVKNDL TNSS ELRSNLEKLVDRI+Y
Sbjct: 1614 RVGNAKTVHPNSLPFYIDSLVDQQLAEERMSSSDVKNDLGTNSSTELRSNLEKLVDRIRY 1673

Query: 841  YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAFE 900
             EVKCANLRDENP+ KSSV+ + G DEKEMSLKELE KLRKLYEQKKQIYKDISIAQAFE
Sbjct: 1674 CEVKCANLRDENPEPKSSVENWMGEDEKEMSLKELESKLRKLYEQKKQIYKDISIAQAFE 1733

Query: 901  KQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDA 960
            K+TNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFDA
Sbjct: 1734 KKTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFDA 1793

Query: 961  VVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1020
            VVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ
Sbjct: 1794 VVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1853

Query: 1021 RAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDIV 1080
            RAGHPVVMLTRQYRMHPEICHFP+QHFYDGKLLNGDGMSGKIAPFHETKGLGPY F+DIV
Sbjct: 1854 RAGHPVVMLTRQYRMHPEICHFPAQHFYDGKLLNGDGMSGKIAPFHETKGLGPYNFFDIV 1913

Query: 1081 DGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRSC 1140
            DGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEFSRVRIGIITPYKCQLSLLRS 
Sbjct: 1914 DGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFSRVRIGIITPYKCQLSLLRSR 1973

Query: 1141 FSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRMN 1200
            FS SFGASLIVDMEFNTVDGFQGREVDILILSTVRA D SSTS  NSSGIGFVADARRMN
Sbjct: 1974 FSHSFGASLIVDMEFNTVDGFQGREVDILILSTVRAVDPSSTSRKNSSGIGFVADARRMN 2033

Query: 1201 VALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQQ 1260
            VALTRAKLSLWVLGNSRTLQVNPDWGAL+KDAKERNLVVSVK PYDS++KTA  R    Q
Sbjct: 2034 VALTRAKLSLWVLGNSRTLQVNPDWGALLKDAKERNLVVSVKKPYDSMFKTATPRYPCPQ 2093

Query: 1261 TMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK-D 1320
            TM NNSRN KH     A RHAKRS KET E EGKDIL  TQCTKTNDVDSS+Y A VK D
Sbjct: 2094 TMTNNSRNPKHTDNVRARRHAKRSGKETFECEGKDIL--TQCTKTNDVDSSRYKAPVKED 2153

Query: 1321 ALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSNS 1380
            A+PLVAGSI+RS K AK+AV +EHGA+FGSKSG S+EKK N GNIS+GKRKVD  KSSN 
Sbjct: 2154 AIPLVAGSIDRSSKAAKSAVRMEHGADFGSKSGKSTEKKFNMGNISQGKRKVDREKSSNF 2213

Query: 1381 DYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRSD 1440
            D++ERG+VDNHALQ S+TSKRLKESP        QE S P+IEGSSKEEHN+  A+SRSD
Sbjct: 2214 DFSERGMVDNHALQKSKTSKRLKESP--------QEASDPLIEGSSKEEHNDGVAISRSD 2273

Query: 1441 TGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRKG 1492
              KELIVKRKQQREAVDAILFSSLIPSKKSEMSMK +SDKKP   SNVRG MKPPKGRKG
Sbjct: 2274 NEKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKHISDKKPHSLSNVRGSMKPPKGRKG 2321

BLAST of Lag0003222 vs. NCBI nr
Match: XP_022145363.1 (uncharacterized protein LOC111014831 [Momordica charantia])

HSP 1 Score: 2354.7 bits (6101), Expect = 0.0e+00
Identity = 1267/1516 (83.58%), Postives = 1351/1516 (89.12%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS I LSG+KM V+D SGFNWLHDLMDWGKSSLKVVLTYW+R
Sbjct: 831  MTCVRLLEIIPIVFERFNPSFIALSGTKMTVKDGSGFNWLHDLMDWGKSSLKVVLTYWKR 890

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDAVVMDELTEKVAHLTILLSKNEKHNVAE 120
            AVISLL+ IKGSCSLSA STIRAI NLIS DDAVV DELTEK+AHLTILLSK EKH++AE
Sbjct: 891  AVISLLNSIKGSCSLSAASTIRAIENLISLDDAVV-DELTEKIAHLTILLSKTEKHHIAE 950

Query: 121  TNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDESK 180
            TNLGSN+  LEDFPSG    TSKS+SF VED+D  TLATR EA+KEDISE+IVLSDDESK
Sbjct: 951  TNLGSNSLVLEDFPSGCKFFTSKSKSFLVEDVDRRTLATRFEAEKEDISEVIVLSDDESK 1010

Query: 181  EYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEINN 240
            EYI PT  F SESD GQ ILV   APS +EN  +ADFGKNKILV E+ KYVVE + EIN+
Sbjct: 1011 EYISPTVAFPSESDAGQCILV---APSGDENDAQADFGKNKILVAEASKYVVEKNNEIND 1070

Query: 241  KCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKALS 300
            K SS +ALK+QAS DSRARPATSSVL+SKDV+ KPREMDS C+      LNDR DLK LS
Sbjct: 1071 KGSSMLALKEQASRDSRARPATSSVLKSKDVNVKPREMDSACI------LNDRNDLKVLS 1130

Query: 301  NKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVPK 360
            ++ATG KSKNQSCETAVS  GYAVLKQVVSDAADDPLE+ELNS+RN+KTNI+K I  VPK
Sbjct: 1131 DEATGYKSKNQSCETAVSAGGYAVLKQVVSDAADDPLELELNSSRNKKTNIAKSIFPVPK 1190

Query: 361  RRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQT 420
            R+VIQLKTPV+NRA++ HRHM+GAKRFKPPRLDDWYRSILELDYFAM+G   VSEDK +T
Sbjct: 1191 RKVIQLKTPVDNRAIHLHRHMVGAKRFKPPRLDDWYRSILELDYFAMVGLASVSEDKIRT 1250

Query: 421  VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVD 480
            VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQL SSFVEMSSWDEMYLGSISVLS+ERVD
Sbjct: 1251 VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLHSSFVEMSSWDEMYLGSISVLSIERVD 1310

Query: 481  EFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNLL 540
            +FHLVRFAYD+NNSVASKSF+ENDLLLLTKELPQKS QGAHMVGKVDRRERDNKRKM LL
Sbjct: 1311 DFHLVRFAYDENNSVASKSFSENDLLLLTKELPQKSPQGAHMVGKVDRRERDNKRKMVLL 1370

Query: 541  IIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPE 600
            IIRFYLLNGS RLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILP ILKPE
Sbjct: 1371 IIRFYLLNGSPRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPTILKPE 1430

Query: 601  ANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 660
            AN+IP  ESKV DLSKLS+PLQQ LKSSFNVSQLQAIDIS GS NM  DLELSL+QGPPG
Sbjct: 1431 ANTIPQHESKVGDLSKLSQPLQQTLKSSFNVSQLQAIDISTGSGNMTKDLELSLIQGPPG 1490

Query: 661  TGKTRTILAIVSALLASASQRTNHA-TSLNRSLKQDNVLHADSRPQISQPVAIARAWQNA 720
            TGKTRTILAIVSALLAS SQRTN A +S+  SLKQDNVLH+DSRP ISQ VA+ARAWQNA
Sbjct: 1491 TGKTRTILAIVSALLASTSQRTNKAKSSVTGSLKQDNVLHSDSRPHISQTVAVARAWQNA 1550

Query: 721  ALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 780
            ALARQLNEDKQR+SK IDC MKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV
Sbjct: 1551 ALARQLNEDKQRSSKSIDCAMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 1610

Query: 781  RVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKY 840
            RVGN KTVHPNSLPFFIDTLVDQQLAEERM+SNDVKND  TNSS ELR NLEKLVDRIKY
Sbjct: 1611 RVGNVKTVHPNSLPFFIDTLVDQQLAEERMSSNDVKNDFGTNSSTELRCNLEKLVDRIKY 1670

Query: 841  YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAFE 900
            YEVKCANLRDENPD KSSV+  KG DE+EMSLKE+ELKLRKLYEQKKQIYKDISIAQAFE
Sbjct: 1671 YEVKCANLRDENPDLKSSVETHKGDDEREMSLKEMELKLRKLYEQKKQIYKDISIAQAFE 1730

Query: 901  KQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDA 960
            K+TNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLY VCAES+LSCKFGSPSENTLFDA
Sbjct: 1731 KKTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYGVCAESMLSCKFGSPSENTLFDA 1790

Query: 961  VVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1020
            VVIDEAAQALEPATLIPLQLLKS A RCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ
Sbjct: 1791 VVIDEAAQALEPATLIPLQLLKSNATRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1850

Query: 1021 RAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDIV 1080
            RAGHPVVMLTRQYRMHPEIC FPSQHFYDGKLLNGDGMSGK  PFHETKGLGPYIF+DIV
Sbjct: 1851 RAGHPVVMLTRQYRMHPEICQFPSQHFYDGKLLNGDGMSGKTVPFHETKGLGPYIFFDIV 1910

Query: 1081 DGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRSC 1140
            DGKELRGKSGGAFSLYN HEADAAVELVKFFK+RHPTEFSRVRIGIITPYKCQLSLLRS 
Sbjct: 1911 DGKELRGKSGGAFSLYNVHEADAAVELVKFFKKRHPTEFSRVRIGIITPYKCQLSLLRSR 1970

Query: 1141 FSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRMN 1200
            FS SFGASL++DMEFNTVDGFQGREVDILILSTVRAAD+SSTSG+NSSGIGFVADARRMN
Sbjct: 1971 FSHSFGASLMIDMEFNTVDGFQGREVDILILSTVRAADSSSTSGMNSSGIGFVADARRMN 2030

Query: 1201 VALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQQ 1260
            VALTRAKLSLWVLGNSRTLQVNPDW ALM+DAKERNLV SVK+PYDS++KT  LR+SY Q
Sbjct: 2031 VALTRAKLSLWVLGNSRTLQVNPDWAALMRDAKERNLVASVKVPYDSMFKTGTLRSSYPQ 2090

Query: 1261 TMDNNSRNLKHAS------RHAKRSRKETSESEGKDILSATQCTKTNDVD---------- 1320
             M NNSRNLKHA        HA+ S KET ESEGKD+++A QCT+T+D +          
Sbjct: 2091 IMANNSRNLKHAENVRSARHHARPSGKETFESEGKDVVAAAQCTRTSDGNLMEARKDIHG 2150

Query: 1321 -----SSQYNASV-KDALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGN 1380
                 +SQ+NASV KD++P VAGSINRS K AKAAV +EHG +FGSKSG  +EKKSN  N
Sbjct: 2151 YKSCATSQHNASVKKDSIPPVAGSINRSSKAAKAAVLVEHGEDFGSKSGRIAEKKSNMVN 2210

Query: 1381 ISRGKRKVDCGKSSNSDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEG 1440
            ISRGKRKVDC KSSN ++AERG+VDNHALQIS TSKRLKESP CDTI  NQE  AP IE 
Sbjct: 2211 ISRGKRKVDCVKSSNLEHAERGMVDNHALQISHTSKRLKESPECDTIRINQEALAPPIEA 2270

Query: 1441 SSKEEHNNSTALSRSDTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-P 1492
            SSKEEH+NS ALS+SDT KE IVKRKQQREAVDAILFSSLIPSKKSEMS K  SDK+P  
Sbjct: 2271 SSKEEHSNSIALSQSDTAKESIVKRKQQREAVDAILFSSLIPSKKSEMSTKHTSDKRPHS 2330

BLAST of Lag0003222 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.4e-82
Identity = 275/903 (30.45%), Postives = 445/903 (49.28%), Query Frame = 0

Query: 343  ARNQKTNISKPITTVPKRRVIQ------LKTPVENRAVYFHRHMIGAKRFKPPRLDDWYR 402
            AR   ++ SK   ++P+RR IQ      LKT          R+   + +    RL+   +
Sbjct: 1004 AREANSHASK---SLPQRRQIQFLDFDSLKTKNVVHPTQLRRNTQQSAQLARLRLNPDVQ 1063

Query: 403  SILELDYFAMIGSTPVSEDKSQTVRHLK--EVPVCFQSSEQYVEIFRPLILEEFKAQLRS 462
                  Y  ++G  P+++  S +   ++  +    +  S  Y ++F+P++  E  AQ++S
Sbjct: 1064 EF----YKVILGWNPLADSFSASNVEMQCVQAKFTYNDSNAYEKVFKPMLFHECWAQVKS 1123

Query: 463  SFVEMSSWDEMYLGSISVLSVERVDEFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQK 522
            + VE   +  +    + + +   VD F  + F       V+  S  + D+ LL+K     
Sbjct: 1124 A-VEEKQYPPI---DLILNTRSTVDNFVDIYFTSCSPTEVSFLS--DTDICLLSKSQSSG 1183

Query: 523  SSQGAHMVGKVDRRERDNKRKMNLLIIRFYLLNGSSRLHQARKNLIERSKWHASRIMSIT 582
             +           +    K++   L +R  +   S  L +   N+    ++ A ++ + T
Sbjct: 1184 DTNNPKSFQLCKIQSISRKKESLELCLRMNI--ESIDLQEYAPNI----RFTAQKLFNAT 1243

Query: 583  PQLREFQALSSIKDIPILPMILKPEANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQ 642
              LREF AL S++ +P+   IL      +P   S   D  K     Q+I+K S+ V++ Q
Sbjct: 1244 TSLREFAALKSLRHLPLSQRILDANVTRLP---SNFTDDKK-----QKIMK-SYGVNEPQ 1303

Query: 643  AIDISIGSRNMKNDLELSLVQGPPGTGKTRTILAIVSALLASASQRTNHATSLNRSLKQD 702
            A  I   S N       +L+QGPPGTGKT+TIL ++ A+L S+SQ               
Sbjct: 1304 AYAIYASSVND----GFTLIQGPPGTGKTKTILGMIGAVLTSSSQ--------------- 1363

Query: 703  NVLHADSRPQISQPVAIARAWQNAALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVD 762
                                        Q N   Q        T K ++LICA SNAA+D
Sbjct: 1364 --------------------------GLQFNVPGQTRK-----TSKNKILICAPSNAAID 1423

Query: 763  ELVSRISNLGLYDSDGKMYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVK 822
            E++ RI   G+YD +G  + P ++RVG   ++  ++  F   TL +Q +  ++M   ++K
Sbjct: 1424 EILLRI-KAGVYDHEGIKFFPKVIRVGFGDSISVHAKEF---TLEEQMI--KQMELTNLK 1483

Query: 823  NDLRTNSSMELRSNLEKLVDRIKYYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELE 882
             D   N+S + R   + ++        K  +LR++    +S+         K  S+  LE
Sbjct: 1484 KDQEANNSSDTRKKYDSIIK-------KRDSLREDLEKFRST--------GKNSSI--LE 1543

Query: 883  LKLRKLYEQKKQIYKDISIAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDL 942
             +LR++ +QK  + + +   +  ++ TN  +  LK +++  +L+EA+IV +TLS  G +L
Sbjct: 1544 AQLREITKQKNMLEQSLDDMRERQRSTNRNLDVLKKQIQNQLLQEADIVCATLSASGHEL 1603

Query: 943  YAVCAESILSCKFGSPSENTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQ 1002
                            +    F  V+IDEAAQA+E +++IP   LK     C+MVGDP Q
Sbjct: 1604 LL--------------NAGLTFRTVIIDEAAQAVELSSIIP---LKYGCESCVMVGDPNQ 1663

Query: 1003 LPATVLSNVASKFLYECSMFERL-QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNG 1062
            LP TVLS  ++KF Y  S++ R+ ++      +L+ QYRM+PEI  FPS+ FY+ KLL+G
Sbjct: 1664 LPPTVLSKTSAKFGYSQSLYVRMFKQHNESACLLSIQYRMNPEISRFPSKFFYNSKLLDG 1723

Query: 1063 DGMSGKIA-PFHETKGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKER 1122
              MS   + P+HE   LG Y F++ V G E    S    SLYN  EA   + L +   + 
Sbjct: 1724 PNMSAVTSRPWHEDPQLGIYRFFN-VHGTEAFSNSK---SLYNVEEASFILLLYERLIQC 1776

Query: 1123 HPTEFSRVRIGIITPYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTV 1182
            +       +IG++TPY+ Q+  LRS F R +G+ +   ++ +TVDGFQG+E DI+I S V
Sbjct: 1784 YLNIDFEGKIGVVTPYRSQVQQLRSQFQRKYGSIIFKHLDIHTVDGFQGQEKDIIIFSCV 1776

Query: 1183 RAADASSTSGINSSGIGFVADARRMNVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKE 1236
            R    SS SG    GIGF+ D RR+NVALTRAK SL+++GNS+ L     + +L++DAK 
Sbjct: 1844 R----SSMSG----GIGFLQDLRRLNVALTRAKSSLYIVGNSKPLMQEDIFYSLIEDAKT 1776

BLAST of Lag0003222 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 6.4e-80
Identity = 261/855 (30.53%), Postives = 409/855 (47.84%), Query Frame = 0

Query: 395  YRSILELDYFAMIGSTPVSE-DKSQTVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLR 454
            Y  IL+ DY      T  SE    + + +  +V   F S   Y ++ +PL+L E    L 
Sbjct: 1117 YEIILQWDY------TRNSEYPDDEPIGNYSDVKDFFNSPADYQKVMKPLLLLESWQGLC 1176

Query: 455  SSFVEMSSWDEMYLGSISVLSVERVDEFHLVRFAYDDNNSVASKSFAENDLLLLT----- 514
            SS       ++    SI V +   V +F+ V +A      +     +E+DL+++      
Sbjct: 1177 SS----RDREDYKPFSIIVGNRTAVSDFYDV-YASVAKQVIQDCGISESDLIVMAYLPDF 1236

Query: 515  ---KELPQKSSQGAHMVGKVDRRERDNKRKMNL-LIIRFYLLNGSSRLHQARKNLIERSK 574
               K L     + A        R   N +  N+ + +R +      R H   K L  RS+
Sbjct: 1237 RPDKRLSSDDFKKAQHTCLAKVRTLKNTKGGNVDVTLRIH------RNHSFSKFLTLRSE 1296

Query: 575  WHASRIMSITPQLREFQALSSIKDIPILPMILKPEANSIPCDESKVVDLSKLSRPLQQIL 634
             +  ++M +T   RE+  L  ++   ++  IL+ + +     ++  ++  K S  L    
Sbjct: 1297 IYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVNVDAAEIETVKKSYKL---- 1356

Query: 635  KSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPGTGKTRTILAIVSALLASASQRTNHA 694
                N SQ +AI  S+           SL+QGPPGTGKT+TIL I+   L+     T +A
Sbjct: 1357 ----NTSQAEAIVNSVSKEG------FSLIQGPPGTGKTKTILGIIGYFLS-----TKNA 1416

Query: 695  TSLNRSLKQDNVLHADSRPQISQPVAIARAWQNAALARQLNEDKQRNSKPIDCTMKRRVL 754
            +S N                I  P+      +N++   QL               K+++L
Sbjct: 1417 SSSN---------------VIKVPLE-----KNSSNTEQL-------------LKKQKIL 1476

Query: 755  ICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLA 814
            ICA SNAAVDE+  R+ + G+YD  G  +KP LVRVG +  V+       ++ LVD+++ 
Sbjct: 1477 ICAPSNAAVDEICLRLKS-GVYDKQGHQFKPQLVRVGRSDVVNVAIKDLTLEELVDKRIG 1536

Query: 815  EERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKYYEVKCANLRDENPDHKSSVQIFKGGD 874
            E          ++RT+  +E + N                N   +  + +  +    G  
Sbjct: 1537 ERNY-------EIRTDPELERKFN----------------NAVTKRRELRGKLDSESGNP 1596

Query: 875  EKEMS---LKELELKLRKLYEQKKQIYKDISIAQAFEKQTNEEVKALKHKLRKSILREAE 934
            E  MS   + +L+LK+R+L +   ++ +D    +             +   +  IL  ++
Sbjct: 1597 ESPMSTEDISKLQLKIRELSKIINELGRDRDEMREKNSVNYRNRDLDRRNAQAHILAVSD 1656

Query: 935  IVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDAVVIDEAAQALEPATLIPLQLLKS 994
            I+ STLSG   D+ A              +    FD V+IDEA Q  E +++IP   L+ 
Sbjct: 1657 IICSTLSGSAHDVLA--------------TMGIKFDTVIIDEACQCTELSSIIP---LRY 1716

Query: 995  RAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQRAGHPVVMLTRQYRMHPEICHFP 1054
               RCIMVGDP QLP TVLS  AS F Y  S+F R+++   P  +L  QYRMHP I  FP
Sbjct: 1717 GGKRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRMEKNSSP-YLLDVQYRMHPSISKFP 1776

Query: 1055 SQHFYDGKLLNGDGMS-GKIAPFHETKGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEAD 1114
            S  FY G+L +G GM      P+H+ + L PY F+DI+ G++   ++    S  N  E  
Sbjct: 1777 SSEFYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFDIISGRQ--EQNAKTMSYTNMEEIR 1836

Query: 1115 AAVELVKFFKERHPTEFSRV-RIGIITPYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGF 1174
             A+ELV +   +   +     +IGII+PY+ Q+  +R  F+R FG  +   ++FNT+DGF
Sbjct: 1837 VAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGF 1851

Query: 1175 QGREVDILILSTVRAADASSTSGINSSGIGFVADARRMNVALTRAKLSLWVLGNSRTLQV 1234
            QG+E +I+++S VRA D  S+       +GF+ D RRMNVALTRAK S+WVLG+ R+L  
Sbjct: 1897 QGQEKEIILISCVRADDTKSS-------VGFLKDFRRMNVALTRAKTSIWVLGHQRSLAK 1851

BLAST of Lag0003222 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 2.1e-78
Identity = 263/892 (29.48%), Postives = 407/892 (45.63%), Query Frame = 0

Query: 394  WYRSILELDYFAMIGSTPVSEDKSQTVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQ-L 453
            +Y  IL  DY   +      +++  +   L  V   ++  + Y E F PL+ EE KAQ L
Sbjct: 19   FYNIILGWDY-KQLTKENERKNRKDSKEKLNVVKNTYKDVDDYFETFEPLLFEEVKAQIL 78

Query: 454  RSSFVEMSSWDEMYLGSISVLSVERVDEFHLVRFAYDDNNSVASKSFAENDLLLLTK-EL 513
            ++   E +S  +M L    V+     + FH +   Y+       +  A+NDLLLL+K E+
Sbjct: 79   QNKDGEEASVCKMRL----VMECNEGEGFHFLLVTYEHEE---DEYLAQNDLLLLSKEEV 138

Query: 514  PQKSSQGAHMVGKVDRRERDNKRKMNLLIIRFYLLNG---------SSRLH---QARKNL 573
               S   ++    V+ R+       NLL +R YL            SSR     QA  N+
Sbjct: 139  KGNSFPSSYGFAVVEHRQN------NLLRLRMYLAEDIVQITKNTKSSRTKSFIQALSNM 198

Query: 574  IERSKWHAS---------RIMSITPQLREFQALSSIKDIPILPMILKPEANSIPCDESKV 633
                   AS         ++  ++  +RE+ AL S+  +P   +I      S    +   
Sbjct: 199  RSLITSSASPIDKRVFSLKLCGLSTIIREYIALRSVSSLPFKDLIFTAAEKSCGFGD--- 258

Query: 634  VDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPGTGKTRTILAIV 693
             +  K+S PL +    + N SQ +AID+ +  ++        L+QGPPGTGKT+TIL+I+
Sbjct: 259  -EAWKISGPLNEFFNENLNKSQKEAIDVGLSRKS------FVLIQGPPGTGKTQTILSIL 318

Query: 694  SALLASA-----SQRTNHATSLNRSLKQDNVLHADSRPQISQPVAIARAWQNAALARQLN 753
             A++ +      S+ T+H       +      +   R   + P  +    ++A +    +
Sbjct: 319  GAIMHATPARVQSKGTDHEVKRGIQMTIQEKYNHWGR---ASPWILGVNPRDAIMPEDGD 378

Query: 754  E------DKQRNSKPIDCTMK--RRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYL 813
            +        +   + ++ + K   RVL+CA SN+A+DE+V R+ + GL D + + Y P +
Sbjct: 379  DGFFPTSGNELKPEVVNASRKYRLRVLVCAPSNSALDEIVLRLLSSGLRDENAQTYTPKI 438

Query: 814  VRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIK 873
            VR+G     H +     +D LV    A++R ++ D      T + ++             
Sbjct: 439  VRIG--LKAHHSVASVSLDHLV----AQKRGSAIDKPKQGTTGTDID------------- 498

Query: 874  YYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAF 933
                                                                        
Sbjct: 499  ------------------------------------------------------------ 558

Query: 934  EKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFD 993
                          +R +IL EA IV +TLS  G  L A                N  FD
Sbjct: 559  -------------SIRTAILEEAAIVFATLSFSGSALLA--------------KSNRGFD 618

Query: 994  AVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1053
             V+IDEAAQA+EPATLIP   L +R  +  +VGDPKQLPATV+S VA    Y  SMFERL
Sbjct: 619  VVIIDEAAQAVEPATLIP---LATRCKQVFLVGDPKQLPATVISTVAQDSGYGTSMFERL 678

Query: 1054 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIA-PFHETKGLGPYIFYD 1113
            Q+AG+PV ML  QYRMHPEI  FPS+ FY+G L +G  +  +    +H+ +  GP+ F+D
Sbjct: 679  QKAGYPVKMLKTQYRMHPEIRSFPSKQFYEGALEDGSDIEAQTTRDWHKYRCFGPFCFFD 738

Query: 1114 IVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLR 1173
            I +GKE +   G   S  N  E +  + +       +P   S  ++ II+PY  Q+   +
Sbjct: 739  IHEGKESQ-HPGATGSRVNLDEVEFVLLIYHRLVTMYPELKSSSQLAIISPYNYQVKTFK 764

Query: 1174 SCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSG-IGFVADAR 1233
              F   FG      ++ NTVDGFQGRE D+ I S VRA         N +G IGF++++R
Sbjct: 799  DRFKEMFGTEAEKVVDINTVDGFQGREKDVAIFSCVRA---------NENGQIGFLSNSR 764

Query: 1234 RMNVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIY 1248
            RMNV +TRAK S+ V+G++ TL+ +P W  L++ A++RN +  V  P ++ +
Sbjct: 859  RMNVGITRAKSSVLVVGSAATLKSDPLWKNLIESAEQRNRLFKVSKPLNNFF 764

BLAST of Lag0003222 vs. ExPASy Swiss-Prot
Match: Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 5.3e-74
Identity = 213/628 (33.92%), Postives = 321/628 (51.11%), Query Frame = 0

Query: 652  SLVQGPPGTGKTRTILAIVSALLASASQRTNHATSLNRSLKQDNVLHADSRPQISQPVAI 711
            +L+QGPPGTGKT+TI+ I+SALL                          SR  I++P   
Sbjct: 1148 TLIQGPPGTGKTKTIIGIISALLVDL-----------------------SRYHITRP--- 1207

Query: 712  ARAWQNAALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGK 771
                               N +      K+++L+CA SNAAVDE++ R+   G    +G+
Sbjct: 1208 -------------------NQQSKSTESKQQILLCAPSNAAVDEVLLRLKR-GFLLENGE 1267

Query: 772  MYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMEL---RSN 831
             Y P +VR+GN +T++ +     ++   ++QL E    + D+       S  EL   R  
Sbjct: 1268 KYIPRVVRIGNPETINVSVRDLSLEYQTEKQLLEVNQGAIDL------GSLQELTRWRDT 1327

Query: 832  LEKLVDRIKYYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIY 891
                + +I+  E +    RD   D KS       G E +  + E  L  +K+ E +    
Sbjct: 1328 FYDCIQKIEELEKQIDVARDVAEDTKSL------GKELQNKINEKNLAEQKVEELQ---- 1387

Query: 892  KDISIAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFG 951
                 +Q+F K  N+EV  L+ K +K+IL++A++V +TLSG G DL A            
Sbjct: 1388 -----SQSFTK--NKEVDLLRKKAQKAILKQADVVCATLSGSGHDLVA------------ 1447

Query: 952  SPSENTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFL 1011
                +  F  V+IDEAAQA+E  T+IP   L+  A +CI+VGDP QLP TVLS  A+   
Sbjct: 1448 --HSSLNFSTVIIDEAAQAVELDTIIP---LRYGAKKCILVGDPNQLPPTVLSKKAASLN 1507

Query: 1012 YECSMFERLQR-AGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAP-FHET 1071
            Y  S+F R+Q+   + + +L+ QYRMHP+I HFPS+ FYD +L +GD M+ K    +H  
Sbjct: 1508 YSQSLFVRIQKNFSNQMCLLSIQYRMHPDISHFPSKKFYDSRLEDGDNMAEKTQQVWHVN 1567

Query: 1072 KGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIIT 1131
                 Y  +D V GKE   ++    S YN  E +  V +V     + P      RIG+IT
Sbjct: 1568 PKFTQYRLFD-VRGKE---RTSNTMSTYNLEEVEYLVNMVDELLNKFPDVNFTGRIGVIT 1627

Query: 1132 PYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSS 1191
            PY+ QL  LR  F   +G S +  ++  TVDGFQG+E DI+  S V++         +  
Sbjct: 1628 PYRSQLHELRRAFKVKYGKSFMSTIDIQTVDGFQGQEKDIIFFSCVKS--------YSKH 1677

Query: 1192 GIGFVADARRMNVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYD-- 1251
            GIGF+ D RR+NVALTRA+ SL ++GN  TL+ +  WG+L+ DA  R LV S  +  +  
Sbjct: 1688 GIGFLRDFRRLNVALTRARSSLLIIGNMETLKTDDLWGSLVDDALSRKLVESPHIDSEGR 1677

Query: 1252 --SIYKTAALRNSYQQTMDNNSRNLKHA 1271
              +I +T+  R   ++ ++  S+ L ++
Sbjct: 1748 LITISRTSEKRMKNEEFVEPPSKKLANS 1677

BLAST of Lag0003222 vs. ExPASy Swiss-Prot
Match: Q86AS0 (Probable helicase DDB_G0274399 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274399 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 1.2e-73
Identity = 279/1058 (26.37%), Postives = 438/1058 (41.40%), Query Frame = 0

Query: 391  LDDWYRSILELDYFAMIGSTPVSEDKSQTVRHLKEVPVCFQSSEQYVEIFRPLILEEFKA 450
            +D +Y+ IL  D          + D S   + LK V V F + E Y+  + PL+ EE +A
Sbjct: 41   IDRFYKHILTWD----------ASDLSPKEKELKPVKVSFNNEEDYITTYEPLLFEECRA 100

Query: 451  QLRSSFVEMSSWDEMYLGSISVLSVERVDEFHLVRFAYDDNNSVASKSFAENDLLLLTKE 510
            QL  S  E    D        V  +  V++F +V     +N ++    F +NDL++++  
Sbjct: 101  QLERSIEEGEKDDTSEPTLSRVRYISEVNDFLVVGLVMAENVNIF--QFHDNDLIMISLH 160

Query: 511  LP------------------QKSSQGAH----------------------------MVGK 570
             P                    +S   H                            ++  
Sbjct: 161  HPLIVFGMDEDEEMTDDEDTAPTSAATHVGAPTKSTTTTTTTTTTTTTTTTTATTNIIDD 220

Query: 571  VDRRERDNKRKMNL----------------------------LIIRFYLLN-GSSRLHQA 630
             ++   D K+K  +                            + ++FY+      R  Q 
Sbjct: 221  PNKTTEDIKKKKKVIPPSKTPITEQNRTLHLIGTVEHLDNGGIKVKFYVKGIKGDRARQV 280

Query: 631  RKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPEANSIPCDESKVVDLSK 690
               L     W  +++ +++   REF AL        +  ++  +      D+ +   + K
Sbjct: 281  SLLLRYEIDWWTTKLCNLSTLQREFAALYQCSQSNFMKTLMMRD------DDGEDGIVMK 340

Query: 691  LSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPGTGKTRTILAIVSALLA 750
            +   L     S++N SQL A+  ++          ++L+QGPPGTGKT  IL ++S LL 
Sbjct: 341  IPPLLHDQFSSTYNDSQLNALTSALEGN------AITLIQGPPGTGKTHVILGLISVLLH 400

Query: 751  S--ASQRTNHATSLNRSLKQDNVLH-ADSRP--QISQPVAIARAWQNAALAR-------- 810
            S    +  +   +L   L +D  L  A+ R    ISQP      W N             
Sbjct: 401  STIVPKVKSGGNNLGDHLLKDRELSMAEKRDLWNISQP------WFNKEFPHIRDNYELI 460

Query: 811  --QLNEDKQRNSKPI-----------DCTMKRRVLICAQSNAAVDELVSRISNLGLYDSD 870
                 E  Q+  + +             + KRR+L+CA SN AVDE+VSR+   GL ++D
Sbjct: 461  DYDFEERDQKRKRDLWRKLRDTGSVKGGSTKRRILLCAPSNGAVDEIVSRLIRDGLLNAD 520

Query: 871  GKMYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNL 930
            G+ Y P LVRVG     H +     +D +V  +  ++ MNSN                  
Sbjct: 521  GRKYNPNLVRVGPGS--HSDVESVSLDYMV--RCRQQLMNSNSA---------------- 580

Query: 931  EKLVDRIKYYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYK 990
                                 P   +S      G  +                      +
Sbjct: 581  --------------------IPSSSASTAAATSGSSRST--------------------Q 640

Query: 991  DISIAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS 1050
            D S                   +R  +L EA+IV +TLS  G  L    A          
Sbjct: 641  DTS------------------SIRTLVLDEADIVATTLSFSGASLLTKMAGG-------- 700

Query: 1051 PSENTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLY 1110
                  FD V+IDEAAQA+E +TLIP+Q       + ++VGDPKQLPAT++S +A K+ Y
Sbjct: 701  ------FDIVIIDEAAQAVETSTLIPIQ---HGCKKVVLVGDPKQLPATIISPLAIKYKY 760

Query: 1111 ECSMFERLQRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGL 1170
            + S+F+RLQ    P  MLT QYRMH  I  FPS+HFY   LL+G  +  +   +H     
Sbjct: 761  DQSLFQRLQEKNSP-HMLTTQYRMHSLIRAFPSRHFYQDLLLDGPNIPSRATHYHSNPFF 820

Query: 1171 GPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYK 1230
            GP +FYD+    E   K GG  S++NEHE   A+ L + F + +P E    RIGII+PY+
Sbjct: 821  GPLVFYDLSWSTET--KPGGG-SVFNEHECKMAMYLFQLFTKVYPDEDFASRIGIISPYR 880

Query: 1231 CQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIG 1290
             Q+  LR  F    G S+      +TVDGFQGRE +I+I S VRA           +GIG
Sbjct: 881  QQVLALREIFKNYPGISI------DTVDGFQGREREIIIFSCVRAPVE------EGAGIG 940

Query: 1291 FVADARRMNVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSV--KMPYDSIY 1328
            F++D RRMNVALTR + SL +LGN++ L +N DW  L++  +    ++ V    P + I 
Sbjct: 941  FLSDVRRMNVALTRPRSSLLILGNTKALSINKDWNELIQHTQNNQQLIPVTKDQPLEIII 957

BLAST of Lag0003222 vs. ExPASy TrEMBL
Match: A0A6J1CVR8 (uncharacterized protein LOC111014831 OS=Momordica charantia OX=3673 GN=LOC111014831 PE=4 SV=1)

HSP 1 Score: 2354.7 bits (6101), Expect = 0.0e+00
Identity = 1267/1516 (83.58%), Postives = 1351/1516 (89.12%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS I LSG+KM V+D SGFNWLHDLMDWGKSSLKVVLTYW+R
Sbjct: 831  MTCVRLLEIIPIVFERFNPSFIALSGTKMTVKDGSGFNWLHDLMDWGKSSLKVVLTYWKR 890

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDAVVMDELTEKVAHLTILLSKNEKHNVAE 120
            AVISLL+ IKGSCSLSA STIRAI NLIS DDAVV DELTEK+AHLTILLSK EKH++AE
Sbjct: 891  AVISLLNSIKGSCSLSAASTIRAIENLISLDDAVV-DELTEKIAHLTILLSKTEKHHIAE 950

Query: 121  TNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDESK 180
            TNLGSN+  LEDFPSG    TSKS+SF VED+D  TLATR EA+KEDISE+IVLSDDESK
Sbjct: 951  TNLGSNSLVLEDFPSGCKFFTSKSKSFLVEDVDRRTLATRFEAEKEDISEVIVLSDDESK 1010

Query: 181  EYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEINN 240
            EYI PT  F SESD GQ ILV   APS +EN  +ADFGKNKILV E+ KYVVE + EIN+
Sbjct: 1011 EYISPTVAFPSESDAGQCILV---APSGDENDAQADFGKNKILVAEASKYVVEKNNEIND 1070

Query: 241  KCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKALS 300
            K SS +ALK+QAS DSRARPATSSVL+SKDV+ KPREMDS C+      LNDR DLK LS
Sbjct: 1071 KGSSMLALKEQASRDSRARPATSSVLKSKDVNVKPREMDSACI------LNDRNDLKVLS 1130

Query: 301  NKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVPK 360
            ++ATG KSKNQSCETAVS  GYAVLKQVVSDAADDPLE+ELNS+RN+KTNI+K I  VPK
Sbjct: 1131 DEATGYKSKNQSCETAVSAGGYAVLKQVVSDAADDPLELELNSSRNKKTNIAKSIFPVPK 1190

Query: 361  RRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQT 420
            R+VIQLKTPV+NRA++ HRHM+GAKRFKPPRLDDWYRSILELDYFAM+G   VSEDK +T
Sbjct: 1191 RKVIQLKTPVDNRAIHLHRHMVGAKRFKPPRLDDWYRSILELDYFAMVGLASVSEDKIRT 1250

Query: 421  VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVD 480
            VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQL SSFVEMSSWDEMYLGSISVLS+ERVD
Sbjct: 1251 VRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLHSSFVEMSSWDEMYLGSISVLSIERVD 1310

Query: 481  EFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNLL 540
            +FHLVRFAYD+NNSVASKSF+ENDLLLLTKELPQKS QGAHMVGKVDRRERDNKRKM LL
Sbjct: 1311 DFHLVRFAYDENNSVASKSFSENDLLLLTKELPQKSPQGAHMVGKVDRRERDNKRKMVLL 1370

Query: 541  IIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKPE 600
            IIRFYLLNGS RLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILP ILKPE
Sbjct: 1371 IIRFYLLNGSPRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPTILKPE 1430

Query: 601  ANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPG 660
            AN+IP  ESKV DLSKLS+PLQQ LKSSFNVSQLQAIDIS GS NM  DLELSL+QGPPG
Sbjct: 1431 ANTIPQHESKVGDLSKLSQPLQQTLKSSFNVSQLQAIDISTGSGNMTKDLELSLIQGPPG 1490

Query: 661  TGKTRTILAIVSALLASASQRTNHA-TSLNRSLKQDNVLHADSRPQISQPVAIARAWQNA 720
            TGKTRTILAIVSALLAS SQRTN A +S+  SLKQDNVLH+DSRP ISQ VA+ARAWQNA
Sbjct: 1491 TGKTRTILAIVSALLASTSQRTNKAKSSVTGSLKQDNVLHSDSRPHISQTVAVARAWQNA 1550

Query: 721  ALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 780
            ALARQLNEDKQR+SK IDC MKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV
Sbjct: 1551 ALARQLNEDKQRSSKSIDCAMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYLV 1610

Query: 781  RVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIKY 840
            RVGN KTVHPNSLPFFIDTLVDQQLAEERM+SNDVKND  TNSS ELR NLEKLVDRIKY
Sbjct: 1611 RVGNVKTVHPNSLPFFIDTLVDQQLAEERMSSNDVKNDFGTNSSTELRCNLEKLVDRIKY 1670

Query: 841  YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAFE 900
            YEVKCANLRDENPD KSSV+  KG DE+EMSLKE+ELKLRKLYEQKKQIYKDISIAQAFE
Sbjct: 1671 YEVKCANLRDENPDLKSSVETHKGDDEREMSLKEMELKLRKLYEQKKQIYKDISIAQAFE 1730

Query: 901  KQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFDA 960
            K+TNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLY VCAES+LSCKFGSPSENTLFDA
Sbjct: 1731 KKTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYGVCAESMLSCKFGSPSENTLFDA 1790

Query: 961  VVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1020
            VVIDEAAQALEPATLIPLQLLKS A RCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ
Sbjct: 1791 VVIDEAAQALEPATLIPLQLLKSNATRCIMVGDPKQLPATVLSNVASKFLYECSMFERLQ 1850

Query: 1021 RAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDIV 1080
            RAGHPVVMLTRQYRMHPEIC FPSQHFYDGKLLNGDGMSGK  PFHETKGLGPYIF+DIV
Sbjct: 1851 RAGHPVVMLTRQYRMHPEICQFPSQHFYDGKLLNGDGMSGKTVPFHETKGLGPYIFFDIV 1910

Query: 1081 DGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRSC 1140
            DGKELRGKSGGAFSLYN HEADAAVELVKFFK+RHPTEFSRVRIGIITPYKCQLSLLRS 
Sbjct: 1911 DGKELRGKSGGAFSLYNVHEADAAVELVKFFKKRHPTEFSRVRIGIITPYKCQLSLLRSR 1970

Query: 1141 FSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRMN 1200
            FS SFGASL++DMEFNTVDGFQGREVDILILSTVRAAD+SSTSG+NSSGIGFVADARRMN
Sbjct: 1971 FSHSFGASLMIDMEFNTVDGFQGREVDILILSTVRAADSSSTSGMNSSGIGFVADARRMN 2030

Query: 1201 VALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQQ 1260
            VALTRAKLSLWVLGNSRTLQVNPDW ALM+DAKERNLV SVK+PYDS++KT  LR+SY Q
Sbjct: 2031 VALTRAKLSLWVLGNSRTLQVNPDWAALMRDAKERNLVASVKVPYDSMFKTGTLRSSYPQ 2090

Query: 1261 TMDNNSRNLKHAS------RHAKRSRKETSESEGKDILSATQCTKTNDVD---------- 1320
             M NNSRNLKHA        HA+ S KET ESEGKD+++A QCT+T+D +          
Sbjct: 2091 IMANNSRNLKHAENVRSARHHARPSGKETFESEGKDVVAAAQCTRTSDGNLMEARKDIHG 2150

Query: 1321 -----SSQYNASV-KDALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGN 1380
                 +SQ+NASV KD++P VAGSINRS K AKAAV +EHG +FGSKSG  +EKKSN  N
Sbjct: 2151 YKSCATSQHNASVKKDSIPPVAGSINRSSKAAKAAVLVEHGEDFGSKSGRIAEKKSNMVN 2210

Query: 1381 ISRGKRKVDCGKSSNSDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEG 1440
            ISRGKRKVDC KSSN ++AERG+VDNHALQIS TSKRLKESP CDTI  NQE  AP IE 
Sbjct: 2211 ISRGKRKVDCVKSSNLEHAERGMVDNHALQISHTSKRLKESPECDTIRINQEALAPPIEA 2270

Query: 1441 SSKEEHNNSTALSRSDTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-P 1492
            SSKEEH+NS ALS+SDT KE IVKRKQQREAVDAILFSSLIPSKKSEMS K  SDK+P  
Sbjct: 2271 SSKEEHSNSIALSQSDTAKESIVKRKQQREAVDAILFSSLIPSKKSEMSTKHTSDKRPHS 2330

BLAST of Lag0003222 vs. ExPASy TrEMBL
Match: A0A1S3CGF3 (uncharacterized protein LOC103500612 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500612 PE=4 SV=1)

HSP 1 Score: 2305.4 bits (5973), Expect = 0.0e+00
Identity = 1247/1501 (83.08%), Postives = 1329/1501 (88.54%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS++ELSG+KM V+D  GFNWLHDLMDWGKSSLKVV+TYWRR
Sbjct: 612  MTCVRLLEIIPIVFERFNPSLVELSGTKMEVKDACGFNWLHDLMDWGKSSLKVVVTYWRR 671

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLIS-SDDAVVMDELTEKVAHLTILLSKNEKHNVA 120
            A+ISLL+FIKGSC LSATSTIRAI +LIS  +DA   DELTEKVAHLTILLSK+EKHN+ 
Sbjct: 672  AIISLLNFIKGSCCLSATSTIRAIEHLISLGEDAAATDELTEKVAHLTILLSKSEKHNIV 731

Query: 121  ETNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDES 180
            +TNL ++A  LEDFPSGR LST+  ES GVED+DV  L    EAKKE   ELIVLSDDES
Sbjct: 732  KTNLQTDALVLEDFPSGRKLSTTTFESPGVEDVDVPMLVNSSEAKKEIFGELIVLSDDES 791

Query: 181  KEYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEIN 240
            K ++ PT  FLSESDVG       +APS NEN TR DFGK+KILV E  KY V+ DQEIN
Sbjct: 792  KPHVSPTRAFLSESDVG-------IAPS-NENDTRGDFGKSKILVVEPSKYAVDRDQEIN 851

Query: 241  NKCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKAL 300
            ++CSST ALK++ASG+S+A PA SS L+SKDVD KP+EMDSEC+L+ +V  N RI+LK L
Sbjct: 852  DQCSSTFALKERASGNSKASPAMSSFLKSKDVDAKPKEMDSECILSKNVAQNGRINLKVL 911

Query: 301  SNKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVP 360
            SNKATGSKSKNQSCETAVSVA  AVLKQVVSDAADDPLEIELNSARNQKTNI KPIT VP
Sbjct: 912  SNKATGSKSKNQSCETAVSVADCAVLKQVVSDAADDPLEIELNSARNQKTNILKPITIVP 971

Query: 361  KRRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQ 420
            KRRVIQLKTP ENRAV+  R MIGAKRFKPPRLDDWYRSILELDYFAMIG T  SEDKS 
Sbjct: 972  KRRVIQLKTPDENRAVHLKRQMIGAKRFKPPRLDDWYRSILELDYFAMIGLTSASEDKSH 1031

Query: 421  TVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERV 480
             V+HL+EVPVCFQS EQYVEIFRPLILEEFKAQLR+SFVE+SSWDEMYLG ISVLSVERV
Sbjct: 1032 MVKHLQEVPVCFQSPEQYVEIFRPLILEEFKAQLRNSFVEISSWDEMYLGKISVLSVERV 1091

Query: 481  DEFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNL 540
            DEFHLVRFAYDDNNSVASK+FAENDL+LLTKE PQKS QGAHMVGKVDRRERDNKRKMNL
Sbjct: 1092 DEFHLVRFAYDDNNSVASKNFAENDLILLTKEPPQKSPQGAHMVGKVDRRERDNKRKMNL 1151

Query: 541  LIIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKP 600
            LIIRFYLL+GSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIP++P IL P
Sbjct: 1152 LIIRFYLLSGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPVVPTILNP 1211

Query: 601  EANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPP 660
            + +SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAID+SIGSRNMKNDLELSLVQGPP
Sbjct: 1212 KTSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDVSIGSRNMKNDLELSLVQGPP 1271

Query: 661  GTGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQN 720
            GTGKTRTILAIVSALLASASQ+TN  A+SLNRSLKQDN     SRP+IS+ VA+ARAWQN
Sbjct: 1272 GTGKTRTILAIVSALLASASQKTNLAASSLNRSLKQDN-----SRPKISEAVAVARAWQN 1331

Query: 721  AALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYL 780
            AALA+QLNEDKQRNS  IDCTMKRRVLICAQSNAAVDELVSRISNLGLYD DGKMYKPYL
Sbjct: 1332 AALAKQLNEDKQRNSISIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDGDGKMYKPYL 1391

Query: 781  VRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIK 840
            VRVGNAKTVHPNSLPF+ID+LVDQ+LAEERMNSND KNDL TNSSMELRSNLEKLVDRI+
Sbjct: 1392 VRVGNAKTVHPNSLPFYIDSLVDQRLAEERMNSNDAKNDLGTNSSMELRSNLEKLVDRIR 1451

Query: 841  YYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAF 900
            YYEVKCANLRDENPD KSSV+   G DEKEMSLKEL+ KLRKLYEQKKQIYKDISIAQAF
Sbjct: 1452 YYEVKCANLRDENPDLKSSVENHAGDDEKEMSLKELQSKLRKLYEQKKQIYKDISIAQAF 1511

Query: 901  EKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFD 960
            EK++NEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFD
Sbjct: 1512 EKKSNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFD 1571

Query: 961  AVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1020
            AVVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL
Sbjct: 1572 AVVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1631

Query: 1021 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDI 1080
            QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGK A FHETKGLGPY+F+DI
Sbjct: 1632 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKNALFHETKGLGPYVFFDI 1691

Query: 1081 VDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRS 1140
            VDGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEF+RVRIGIITPYKCQLSLLRS
Sbjct: 1692 VDGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFNRVRIGIITPYKCQLSLLRS 1751

Query: 1141 CFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRM 1200
             FS SFGASL+VDMEFNTVDGFQGREVDILILSTVRA D+S  SG NSSGIGFVADARRM
Sbjct: 1752 RFSHSFGASLVVDMEFNTVDGFQGREVDILILSTVRAGDSSPHSGKNSSGIGFVADARRM 1811

Query: 1201 NVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQ 1260
            NVALTRAKLSLWVLGNSRTL VNPDWGAL+KDAKERNLVVSVK PYDS++KT  LRNS  
Sbjct: 1812 NVALTRAKLSLWVLGNSRTLHVNPDWGALLKDAKERNLVVSVKKPYDSMFKTTNLRNSNL 1871

Query: 1261 QTMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK- 1320
            QT +NNS+  KH     A  HAKRS K+T ESEGKD  + TQCTKTND+DSSQ NASVK 
Sbjct: 1872 QTTENNSKTPKHTDNVRARHHAKRSGKKTFESEGKD--TPTQCTKTNDIDSSQDNASVKE 1931

Query: 1321 DALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSN 1380
            DA+P VAG INR  K AK AV +EHG +F SKSG S+EKK N  N S GKRKVD  KSSN
Sbjct: 1932 DAIPPVAGGINRPSKAAKGAVRMEHGRDFESKSGKSAEKKFNKCNTSHGKRKVDREKSSN 1991

Query: 1381 SDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRS 1440
             D +ERG VDNH      TSKR KESP  DTICTN E SAP++E SSKEE NNS A SR 
Sbjct: 1992 FDNSERGKVDNH------TSKRPKESPQHDTICTNLESSAPLVEESSKEERNNSAAPSRC 2051

Query: 1441 DTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRK 1492
            DT KELIVKRK+QREAVDAILFSSLIPSKKSEMSMKL+SDKKP   SNVRG MKPPKGRK
Sbjct: 2052 DTEKELIVKRKKQREAVDAILFSSLIPSKKSEMSMKLISDKKPHSLSNVRGSMKPPKGRK 2091

BLAST of Lag0003222 vs. ExPASy TrEMBL
Match: A0A1S4E3Q3 (uncharacterized protein LOC103500612 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500612 PE=4 SV=1)

HSP 1 Score: 2305.4 bits (5973), Expect = 0.0e+00
Identity = 1247/1501 (83.08%), Postives = 1329/1501 (88.54%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS++ELSG+KM V+D  GFNWLHDLMDWGKSSLKVV+TYWRR
Sbjct: 661  MTCVRLLEIIPIVFERFNPSLVELSGTKMEVKDACGFNWLHDLMDWGKSSLKVVVTYWRR 720

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLIS-SDDAVVMDELTEKVAHLTILLSKNEKHNVA 120
            A+ISLL+FIKGSC LSATSTIRAI +LIS  +DA   DELTEKVAHLTILLSK+EKHN+ 
Sbjct: 721  AIISLLNFIKGSCCLSATSTIRAIEHLISLGEDAAATDELTEKVAHLTILLSKSEKHNIV 780

Query: 121  ETNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDES 180
            +TNL ++A  LEDFPSGR LST+  ES GVED+DV  L    EAKKE   ELIVLSDDES
Sbjct: 781  KTNLQTDALVLEDFPSGRKLSTTTFESPGVEDVDVPMLVNSSEAKKEIFGELIVLSDDES 840

Query: 181  KEYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEIN 240
            K ++ PT  FLSESDVG       +APS NEN TR DFGK+KILV E  KY V+ DQEIN
Sbjct: 841  KPHVSPTRAFLSESDVG-------IAPS-NENDTRGDFGKSKILVVEPSKYAVDRDQEIN 900

Query: 241  NKCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKAL 300
            ++CSST ALK++ASG+S+A PA SS L+SKDVD KP+EMDSEC+L+ +V  N RI+LK L
Sbjct: 901  DQCSSTFALKERASGNSKASPAMSSFLKSKDVDAKPKEMDSECILSKNVAQNGRINLKVL 960

Query: 301  SNKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVP 360
            SNKATGSKSKNQSCETAVSVA  AVLKQVVSDAADDPLEIELNSARNQKTNI KPIT VP
Sbjct: 961  SNKATGSKSKNQSCETAVSVADCAVLKQVVSDAADDPLEIELNSARNQKTNILKPITIVP 1020

Query: 361  KRRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQ 420
            KRRVIQLKTP ENRAV+  R MIGAKRFKPPRLDDWYRSILELDYFAMIG T  SEDKS 
Sbjct: 1021 KRRVIQLKTPDENRAVHLKRQMIGAKRFKPPRLDDWYRSILELDYFAMIGLTSASEDKSH 1080

Query: 421  TVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERV 480
             V+HL+EVPVCFQS EQYVEIFRPLILEEFKAQLR+SFVE+SSWDEMYLG ISVLSVERV
Sbjct: 1081 MVKHLQEVPVCFQSPEQYVEIFRPLILEEFKAQLRNSFVEISSWDEMYLGKISVLSVERV 1140

Query: 481  DEFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNL 540
            DEFHLVRFAYDDNNSVASK+FAENDL+LLTKE PQKS QGAHMVGKVDRRERDNKRKMNL
Sbjct: 1141 DEFHLVRFAYDDNNSVASKNFAENDLILLTKEPPQKSPQGAHMVGKVDRRERDNKRKMNL 1200

Query: 541  LIIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKP 600
            LIIRFYLL+GSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIP++P IL P
Sbjct: 1201 LIIRFYLLSGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPVVPTILNP 1260

Query: 601  EANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPP 660
            + +SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAID+SIGSRNMKNDLELSLVQGPP
Sbjct: 1261 KTSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDVSIGSRNMKNDLELSLVQGPP 1320

Query: 661  GTGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQN 720
            GTGKTRTILAIVSALLASASQ+TN  A+SLNRSLKQDN     SRP+IS+ VA+ARAWQN
Sbjct: 1321 GTGKTRTILAIVSALLASASQKTNLAASSLNRSLKQDN-----SRPKISEAVAVARAWQN 1380

Query: 721  AALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYL 780
            AALA+QLNEDKQRNS  IDCTMKRRVLICAQSNAAVDELVSRISNLGLYD DGKMYKPYL
Sbjct: 1381 AALAKQLNEDKQRNSISIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDGDGKMYKPYL 1440

Query: 781  VRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIK 840
            VRVGNAKTVHPNSLPF+ID+LVDQ+LAEERMNSND KNDL TNSSMELRSNLEKLVDRI+
Sbjct: 1441 VRVGNAKTVHPNSLPFYIDSLVDQRLAEERMNSNDAKNDLGTNSSMELRSNLEKLVDRIR 1500

Query: 841  YYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAF 900
            YYEVKCANLRDENPD KSSV+   G DEKEMSLKEL+ KLRKLYEQKKQIYKDISIAQAF
Sbjct: 1501 YYEVKCANLRDENPDLKSSVENHAGDDEKEMSLKELQSKLRKLYEQKKQIYKDISIAQAF 1560

Query: 901  EKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFD 960
            EK++NEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFD
Sbjct: 1561 EKKSNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFD 1620

Query: 961  AVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1020
            AVVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL
Sbjct: 1621 AVVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1680

Query: 1021 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDI 1080
            QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGK A FHETKGLGPY+F+DI
Sbjct: 1681 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKNALFHETKGLGPYVFFDI 1740

Query: 1081 VDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRS 1140
            VDGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEF+RVRIGIITPYKCQLSLLRS
Sbjct: 1741 VDGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFNRVRIGIITPYKCQLSLLRS 1800

Query: 1141 CFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRM 1200
             FS SFGASL+VDMEFNTVDGFQGREVDILILSTVRA D+S  SG NSSGIGFVADARRM
Sbjct: 1801 RFSHSFGASLVVDMEFNTVDGFQGREVDILILSTVRAGDSSPHSGKNSSGIGFVADARRM 1860

Query: 1201 NVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQ 1260
            NVALTRAKLSLWVLGNSRTL VNPDWGAL+KDAKERNLVVSVK PYDS++KT  LRNS  
Sbjct: 1861 NVALTRAKLSLWVLGNSRTLHVNPDWGALLKDAKERNLVVSVKKPYDSMFKTTNLRNSNL 1920

Query: 1261 QTMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK- 1320
            QT +NNS+  KH     A  HAKRS K+T ESEGKD  + TQCTKTND+DSSQ NASVK 
Sbjct: 1921 QTTENNSKTPKHTDNVRARHHAKRSGKKTFESEGKD--TPTQCTKTNDIDSSQDNASVKE 1980

Query: 1321 DALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSN 1380
            DA+P VAG INR  K AK AV +EHG +F SKSG S+EKK N  N S GKRKVD  KSSN
Sbjct: 1981 DAIPPVAGGINRPSKAAKGAVRMEHGRDFESKSGKSAEKKFNKCNTSHGKRKVDREKSSN 2040

Query: 1381 SDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRS 1440
             D +ERG VDNH      TSKR KESP  DTICTN E SAP++E SSKEE NNS A SR 
Sbjct: 2041 FDNSERGKVDNH------TSKRPKESPQHDTICTNLESSAPLVEESSKEERNNSAAPSRC 2100

Query: 1441 DTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRK 1492
            DT KELIVKRK+QREAVDAILFSSLIPSKKSEMSMKL+SDKKP   SNVRG MKPPKGRK
Sbjct: 2101 DTEKELIVKRKKQREAVDAILFSSLIPSKKSEMSMKLISDKKPHSLSNVRGSMKPPKGRK 2140

BLAST of Lag0003222 vs. ExPASy TrEMBL
Match: A0A1S3CGD9 (uncharacterized protein LOC103500612 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500612 PE=4 SV=1)

HSP 1 Score: 2305.4 bits (5973), Expect = 0.0e+00
Identity = 1247/1501 (83.08%), Postives = 1329/1501 (88.54%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS++ELSG+KM V+D  GFNWLHDLMDWGKSSLKVV+TYWRR
Sbjct: 833  MTCVRLLEIIPIVFERFNPSLVELSGTKMEVKDACGFNWLHDLMDWGKSSLKVVVTYWRR 892

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLIS-SDDAVVMDELTEKVAHLTILLSKNEKHNVA 120
            A+ISLL+FIKGSC LSATSTIRAI +LIS  +DA   DELTEKVAHLTILLSK+EKHN+ 
Sbjct: 893  AIISLLNFIKGSCCLSATSTIRAIEHLISLGEDAAATDELTEKVAHLTILLSKSEKHNIV 952

Query: 121  ETNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDES 180
            +TNL ++A  LEDFPSGR LST+  ES GVED+DV  L    EAKKE   ELIVLSDDES
Sbjct: 953  KTNLQTDALVLEDFPSGRKLSTTTFESPGVEDVDVPMLVNSSEAKKEIFGELIVLSDDES 1012

Query: 181  KEYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEIN 240
            K ++ PT  FLSESDVG       +APS NEN TR DFGK+KILV E  KY V+ DQEIN
Sbjct: 1013 KPHVSPTRAFLSESDVG-------IAPS-NENDTRGDFGKSKILVVEPSKYAVDRDQEIN 1072

Query: 241  NKCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKAL 300
            ++CSST ALK++ASG+S+A PA SS L+SKDVD KP+EMDSEC+L+ +V  N RI+LK L
Sbjct: 1073 DQCSSTFALKERASGNSKASPAMSSFLKSKDVDAKPKEMDSECILSKNVAQNGRINLKVL 1132

Query: 301  SNKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVP 360
            SNKATGSKSKNQSCETAVSVA  AVLKQVVSDAADDPLEIELNSARNQKTNI KPIT VP
Sbjct: 1133 SNKATGSKSKNQSCETAVSVADCAVLKQVVSDAADDPLEIELNSARNQKTNILKPITIVP 1192

Query: 361  KRRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQ 420
            KRRVIQLKTP ENRAV+  R MIGAKRFKPPRLDDWYRSILELDYFAMIG T  SEDKS 
Sbjct: 1193 KRRVIQLKTPDENRAVHLKRQMIGAKRFKPPRLDDWYRSILELDYFAMIGLTSASEDKSH 1252

Query: 421  TVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERV 480
             V+HL+EVPVCFQS EQYVEIFRPLILEEFKAQLR+SFVE+SSWDEMYLG ISVLSVERV
Sbjct: 1253 MVKHLQEVPVCFQSPEQYVEIFRPLILEEFKAQLRNSFVEISSWDEMYLGKISVLSVERV 1312

Query: 481  DEFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNL 540
            DEFHLVRFAYDDNNSVASK+FAENDL+LLTKE PQKS QGAHMVGKVDRRERDNKRKMNL
Sbjct: 1313 DEFHLVRFAYDDNNSVASKNFAENDLILLTKEPPQKSPQGAHMVGKVDRRERDNKRKMNL 1372

Query: 541  LIIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKP 600
            LIIRFYLL+GSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIP++P IL P
Sbjct: 1373 LIIRFYLLSGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPVVPTILNP 1432

Query: 601  EANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPP 660
            + +SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAID+SIGSRNMKNDLELSLVQGPP
Sbjct: 1433 KTSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDVSIGSRNMKNDLELSLVQGPP 1492

Query: 661  GTGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQN 720
            GTGKTRTILAIVSALLASASQ+TN  A+SLNRSLKQDN     SRP+IS+ VA+ARAWQN
Sbjct: 1493 GTGKTRTILAIVSALLASASQKTNLAASSLNRSLKQDN-----SRPKISEAVAVARAWQN 1552

Query: 721  AALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYL 780
            AALA+QLNEDKQRNS  IDCTMKRRVLICAQSNAAVDELVSRISNLGLYD DGKMYKPYL
Sbjct: 1553 AALAKQLNEDKQRNSISIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDGDGKMYKPYL 1612

Query: 781  VRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIK 840
            VRVGNAKTVHPNSLPF+ID+LVDQ+LAEERMNSND KNDL TNSSMELRSNLEKLVDRI+
Sbjct: 1613 VRVGNAKTVHPNSLPFYIDSLVDQRLAEERMNSNDAKNDLGTNSSMELRSNLEKLVDRIR 1672

Query: 841  YYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAF 900
            YYEVKCANLRDENPD KSSV+   G DEKEMSLKEL+ KLRKLYEQKKQIYKDISIAQAF
Sbjct: 1673 YYEVKCANLRDENPDLKSSVENHAGDDEKEMSLKELQSKLRKLYEQKKQIYKDISIAQAF 1732

Query: 901  EKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFD 960
            EK++NEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFD
Sbjct: 1733 EKKSNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFD 1792

Query: 961  AVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1020
            AVVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL
Sbjct: 1793 AVVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1852

Query: 1021 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDI 1080
            QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGK A FHETKGLGPY+F+DI
Sbjct: 1853 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKNALFHETKGLGPYVFFDI 1912

Query: 1081 VDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRS 1140
            VDGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEF+RVRIGIITPYKCQLSLLRS
Sbjct: 1913 VDGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFNRVRIGIITPYKCQLSLLRS 1972

Query: 1141 CFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRM 1200
             FS SFGASL+VDMEFNTVDGFQGREVDILILSTVRA D+S  SG NSSGIGFVADARRM
Sbjct: 1973 RFSHSFGASLVVDMEFNTVDGFQGREVDILILSTVRAGDSSPHSGKNSSGIGFVADARRM 2032

Query: 1201 NVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQ 1260
            NVALTRAKLSLWVLGNSRTL VNPDWGAL+KDAKERNLVVSVK PYDS++KT  LRNS  
Sbjct: 2033 NVALTRAKLSLWVLGNSRTLHVNPDWGALLKDAKERNLVVSVKKPYDSMFKTTNLRNSNL 2092

Query: 1261 QTMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK- 1320
            QT +NNS+  KH     A  HAKRS K+T ESEGKD  + TQCTKTND+DSSQ NASVK 
Sbjct: 2093 QTTENNSKTPKHTDNVRARHHAKRSGKKTFESEGKD--TPTQCTKTNDIDSSQDNASVKE 2152

Query: 1321 DALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSN 1380
            DA+P VAG INR  K AK AV +EHG +F SKSG S+EKK N  N S GKRKVD  KSSN
Sbjct: 2153 DAIPPVAGGINRPSKAAKGAVRMEHGRDFESKSGKSAEKKFNKCNTSHGKRKVDREKSSN 2212

Query: 1381 SDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRS 1440
             D +ERG VDNH      TSKR KESP  DTICTN E SAP++E SSKEE NNS A SR 
Sbjct: 2213 FDNSERGKVDNH------TSKRPKESPQHDTICTNLESSAPLVEESSKEERNNSAAPSRC 2272

Query: 1441 DTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRK 1492
            DT KELIVKRK+QREAVDAILFSSLIPSKKSEMSMKL+SDKKP   SNVRG MKPPKGRK
Sbjct: 2273 DTEKELIVKRKKQREAVDAILFSSLIPSKKSEMSMKLISDKKPHSLSNVRGSMKPPKGRK 2312

BLAST of Lag0003222 vs. ExPASy TrEMBL
Match: A0A5A7V0K3 (Helicase SEN1 isoform X4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G00530 PE=4 SV=1)

HSP 1 Score: 2300.4 bits (5960), Expect = 0.0e+00
Identity = 1245/1501 (82.94%), Postives = 1327/1501 (88.41%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEIIPIVFERFNPS++ELSG+KM V+D  GFNWLHDLMDWGKSSLKVV+TYWRR
Sbjct: 844  MTCVRLLEIIPIVFERFNPSLVELSGTKMEVKDACGFNWLHDLMDWGKSSLKVVVTYWRR 903

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLIS-SDDAVVMDELTEKVAHLTILLSKNEKHNVA 120
            A+ISLL+FIKGSC LSATSTIRAI +LIS  +DA   DELTEKVAHLTILLSK+EKHN+ 
Sbjct: 904  AIISLLNFIKGSCCLSATSTIRAIEHLISLGEDAAATDELTEKVAHLTILLSKSEKHNIV 963

Query: 121  ETNLGSNA-KLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDISELIVLSDDES 180
            +TNL ++A  LEDFPSGR LST+  ES GVED+DV  L    EAKKE   ELIVLSDDES
Sbjct: 964  KTNLQTDALVLEDFPSGRKLSTTAFESPGVEDVDVPMLVNSSEAKKEIFGELIVLSDDES 1023

Query: 181  KEYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLKYVVETDQEIN 240
            K ++ PT  FLSESDVG       +APS NEN TR DFGK+KILV E  KY V+ DQEIN
Sbjct: 1024 KPHVSPTRAFLSESDVG-------IAPS-NENDTRGDFGKSKILVVEPSKYAVDRDQEIN 1083

Query: 241  NKCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVTLNDRIDLKAL 300
            ++CSST ALK++ASG+S+A PA SS L+SKDVD KP+EMDSEC+L+ +V  N RI+LK L
Sbjct: 1084 DQCSSTFALKERASGNSKASPAMSSFLKSKDVDAKPKEMDSECILSKNVAQNGRINLKVL 1143

Query: 301  SNKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKTNISKPITTVP 360
            SNKATGSKSKNQSCETAVSVA  AVLKQVVSDAADDPLEIELNSARNQKTNI KPIT VP
Sbjct: 1144 SNKATGSKSKNQSCETAVSVADCAVLKQVVSDAADDPLEIELNSARNQKTNILKPITIVP 1203

Query: 361  KRRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIGSTPVSEDKSQ 420
            KRRVIQLKTP ENRAV+  R MIGAKRFKPPRLDDWYRSILELDYFAMIG T  SEDKS 
Sbjct: 1204 KRRVIQLKTPDENRAVHLKRQMIGAKRFKPPRLDDWYRSILELDYFAMIGLTSASEDKSH 1263

Query: 421  TVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERV 480
             V+HL+EVPVCFQS EQYVEIFRPLILEEFKAQLR+SFVE+SSWDEMYLG ISVLSVERV
Sbjct: 1264 MVKHLQEVPVCFQSPEQYVEIFRPLILEEFKAQLRNSFVEISSWDEMYLGKISVLSVERV 1323

Query: 481  DEFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRRERDNKRKMNL 540
            DEFHLVRFAYDDNNSVASK+FAENDL+LLTKE PQKS QGAHMVGKVDRRERDNKRKMNL
Sbjct: 1324 DEFHLVRFAYDDNNSVASKNFAENDLILLTKEPPQKSPQGAHMVGKVDRRERDNKRKMNL 1383

Query: 541  LIIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPILPMILKP 600
            LIIRFYLL+GSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIP++P IL P
Sbjct: 1384 LIIRFYLLSGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKDIPVVPTILNP 1443

Query: 601  EANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPP 660
            + +SIP DESKVVDLSKLSRPLQQILKSSFNVSQLQAID+SIGSRNMKNDLELSLVQGPP
Sbjct: 1444 KTSSIPHDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDVSIGSRNMKNDLELSLVQGPP 1503

Query: 661  GTGKTRTILAIVSALLASASQRTN-HATSLNRSLKQDNVLHADSRPQISQPVAIARAWQN 720
            GTGKTRTILAIVSALLASASQ+TN  A+SLNRSLKQDN     SRP+IS+ VA+ARAWQN
Sbjct: 1504 GTGKTRTILAIVSALLASASQKTNLAASSLNRSLKQDN-----SRPKISEAVAVARAWQN 1563

Query: 721  AALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYL 780
            AALA+QLN DKQRNS  IDCTMKRRVLICAQSNAAVDELVSRISNLGLYD DGKMYKPYL
Sbjct: 1564 AALAKQLNADKQRNSISIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDGDGKMYKPYL 1623

Query: 781  VRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIK 840
            VRVGNAKTVHPNSLPF+ID+LVDQ+LAEERMNSND KNDL TNSSMELRSNLEKLVDRI+
Sbjct: 1624 VRVGNAKTVHPNSLPFYIDSLVDQRLAEERMNSNDAKNDLGTNSSMELRSNLEKLVDRIR 1683

Query: 841  YYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAF 900
            YYEVKCANLRDENPD KSSV+   G DEKEMSLKEL+ KLRKLYEQKKQIYKDISIAQAF
Sbjct: 1684 YYEVKCANLRDENPDLKSSVENHAGDDEKEMSLKELQSKLRKLYEQKKQIYKDISIAQAF 1743

Query: 901  EKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFD 960
            EK++NEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGS SENTLFD
Sbjct: 1744 EKKSNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSSSENTLFD 1803

Query: 961  AVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1020
            AVVIDEAAQALEPATLIPLQLLKS AIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL
Sbjct: 1804 AVVIDEAAQALEPATLIPLQLLKSSAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1863

Query: 1021 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHETKGLGPYIFYDI 1080
            QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGK A FHETKGLGPY+F+DI
Sbjct: 1864 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKNALFHETKGLGPYVFFDI 1923

Query: 1081 VDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLRS 1140
            VDGKELR KSGGAFSLYNEHEADAAVELVKFFKE HPTEF+RVRIGIITPYKCQLSLLRS
Sbjct: 1924 VDGKELRSKSGGAFSLYNEHEADAAVELVKFFKESHPTEFNRVRIGIITPYKCQLSLLRS 1983

Query: 1141 CFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSGIGFVADARRM 1200
             FS SFGASL+VDMEFNTVDGFQGREVDILILSTVRA D+S  SG NSSGIGFVADARRM
Sbjct: 1984 RFSHSFGASLVVDMEFNTVDGFQGREVDILILSTVRAGDSSPHSGKNSSGIGFVADARRM 2043

Query: 1201 NVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIYKTAALRNSYQ 1260
            NVALTRAKLSLWVLGNSRTL VNPDWGAL+KDAKERNLVVSVK PYDS++KT  LRNS  
Sbjct: 2044 NVALTRAKLSLWVLGNSRTLHVNPDWGALLKDAKERNLVVSVKKPYDSMFKTTNLRNSNL 2103

Query: 1261 QTMDNNSRNLKH-----ASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQYNASVK- 1320
            QT +NNS+  KH     A  HAKRS K+T ESEGKD  + TQCTKTND+DSSQ NASVK 
Sbjct: 2104 QTTENNSKTPKHTDNVRARHHAKRSGKKTFESEGKD--TPTQCTKTNDIDSSQDNASVKE 2163

Query: 1321 DALPLVAGSINRSLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRKVDCGKSSN 1380
            DA+P VAG INR  K AK AV +EH  +F SKSG S+EKK N  N S GKRKVD  KSSN
Sbjct: 2164 DAIPPVAGGINRPSKAAKGAVRMEHDRDFESKSGKSAEKKFNKCNTSHGKRKVDREKSSN 2223

Query: 1381 SDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHNNSTALSRS 1440
             D +ERG VDNH      TSKR KESP  DTICTN E SAP++E SSKEE NNS A SR 
Sbjct: 2224 FDNSERGKVDNH------TSKRPKESPQHDTICTNLESSAPLVEESSKEERNNSAAPSRC 2283

Query: 1441 DTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKP-PFSNVRGCMKPPKGRK 1492
            DT KELIVKRK+QREAVDAILFSSLIPSKKSEMSMKL+SDKKP   SNVRG MKPPKGRK
Sbjct: 2284 DTEKELIVKRKKQREAVDAILFSSLIPSKKSEMSMKLISDKKPHSLSNVRGSMKPPKGRK 2323

BLAST of Lag0003222 vs. TAIR 10
Match: AT1G16800.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 715/1507 (47.45%), Postives = 977/1507 (64.83%), Query Frame = 0

Query: 1    MTCVRLLEIIPIVFERFNPSVIELSGSKMAVQDTSGFNWLHDLMDWGKSSLKVVLTYWRR 60
            MTCVRLLEI+P+V  +   S  E   ++  ++D S   WL DL+DWG+S LKVV+ YW+R
Sbjct: 707  MTCVRLLEILPVVLGKLRVSREESCDTRGTLKDASDLKWLPDLIDWGRSQLKVVVAYWKR 766

Query: 61   AVISLLSFIKGSCSLSATSTIRAIGNLISSDDA------------VVMDELTEKVAHLTI 120
            A+++LL  ++GS S + +S ++AI +++SS D             V +++L E+++    
Sbjct: 767  ALVALLDILQGSNSDACSSAVQAIRHVLSSGDTIDNALTLLNSDDVDIEQLAEQISR--- 826

Query: 121  LLSKNEKHNVAETNLGSNAKLEDFPSGRNLSTSKSESFGVEDIDVLTLATRPEAKKEDIS 180
            L+ K  ++ + +  +    KL+D  +  +L+  ++E   ++++  L  + +P+  K    
Sbjct: 827  LVPKANEYQILKP-VDVVGKLQD--NMMDLTVDETEKESLKNLPSLHKSHQPDINKT--- 886

Query: 181  ELIVLSDDESKEYILPTGDFLSESDVGQGILVGNLAPSVNENYTRADFGKNKILVTESLK 240
                         +LP  +    S             S+ ++ +  D  K   LV  S +
Sbjct: 887  -------------LLPIKNISQIS-------------SLKKSTSSIDASKLSALVL-SER 946

Query: 241  YVVETDQEINNKCSSTVALKDQASGDSRARPATSSVLRSKDVDFKPREMDSECVLNDHVT 300
             V  +   I     +T A   +A+G S                   RE +    + D V+
Sbjct: 947  DVTVSSSNIVRDLPTTNAEPSKAAGMS-------------------REAEKRQNVEDPVS 1006

Query: 301  LNDRIDLKALSNKATGSKSKNQSCETAVSVAGYAVLKQVVSDAADDPLEIELNSARNQKT 360
               R +LK  +++     +  ++ ++A+S A    L++VV++   DPL++ L S + Q  
Sbjct: 1007 SGIRPNLKKATDELGPRGTSKEAQKSAISNAKGMDLRKVVNETEVDPLDLALKSLKRQSL 1066

Query: 361  NISKPITTVPKRRVIQLKTPVENRAVYFHRHMIGAKRFKPPRLDDWYRSILELDYFAMIG 420
             ++K    VPKR+VIQL  PV  ++  + R   G KRF+PP+L+DW+R IL++DY+A++G
Sbjct: 1067 PLAKSGPIVPKRQVIQLCAPVNKKSDRWQRQEAGFKRFRPPKLEDWFRKILQMDYYAIVG 1126

Query: 421  STPVSEDKSQTVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLG 480
                 +D+SQ V   +EVPV F S EQY++IF+PL+LEEFKAQL+SSF E+SS +E+Y G
Sbjct: 1127 LASTKKDESQNVGKFREVPVRFGSPEQYIQIFQPLVLEEFKAQLQSSFQEISSLEEIYYG 1186

Query: 481  SISVLSVERVDEFHLVRFAYDDNNSVASKSFAENDLLLLTKELPQKSSQGAHMVGKVDRR 540
             ISVLS+ERVD+FH VRF  D+N+   SKSF+ENDL+L TKE P+ S+ G +M+GKV+ R
Sbjct: 1187 VISVLSIERVDDFHFVRFMQDENDGSNSKSFSENDLVLFTKEHPENSNVGVNMMGKVEGR 1246

Query: 541  ERDNKRKMNLLIIRFYLLNGSSRLHQARKNLIERSKWHASRIMSITPQLREFQALSSIKD 600
            E D+K++ ++L +R YL N SSRL+QAR+NL+ERS+WHASRI++IT Q+REFQALS IKD
Sbjct: 1247 EWDDKKRTSILNVRLYLQNASSRLNQARRNLLERSQWHASRILNITSQIREFQALSCIKD 1306

Query: 601  IPILPMILKPEANSIPCDESKVVDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKND 660
            IP+LP+IL P  +S    E K  DL  L   LQQILKSSFN SQLQAI ++IGS N+   
Sbjct: 1307 IPVLPLILSPMNDSNYDSEVKRSDLRSLPHSLQQILKSSFNESQLQAISVAIGSSNLMKA 1366

Query: 661  LELSLVQGPPGTGKTRTILAIVSALLASASQRTNHATSLNRSLKQDNVLHADSRPQISQP 720
             ++SL+QGPPGTGKTRTI+AI+S LLASAS +T+     +R   +     + SR  ++  
Sbjct: 1367 FDISLIQGPPGTGKTRTIVAIISGLLASASHKTS-----DRGNSEPGHSSSTSRQGMNPS 1426

Query: 721  VAIARAWQNAALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAAVDELVSRISNLGLYDS 780
            VA+ARAWQ+AALA+QLN++ + N K  +   + RVLICAQSNAAVDELVSRIS+LG+Y  
Sbjct: 1427 VAVARAWQDAALAKQLNDNSETNRKIAEKNGRGRVLICAQSNAAVDELVSRISSLGIYGR 1486

Query: 781  DGKMYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSN 840
            DGKM+KPYLVRVGNAKTVH NS+PFF+DTLVDQ+LAEERM  N+ K++   +SS  LRSN
Sbjct: 1487 DGKMFKPYLVRVGNAKTVHSNSMPFFLDTLVDQRLAEERMRINESKSNKGADSSALLRSN 1546

Query: 841  LEKLVDRIKYYEVKCANLRDENPDHKSSVQ---IFKGGDEKEMSLKELELKLRKLYEQKK 900
            LEK+VD+I ++E K AN+  E+ D K   +     K  D K MS  EL ++LR+LYEQK+
Sbjct: 1547 LEKVVDQITHFEAKRANINQESLDAKDKPENEHHNKDDDGKLMSDAELGIRLRRLYEQKR 1606

Query: 901  QIYKDISIAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSC 960
            +IYKD+S  QA E++ N E++ LK KLRKSIL+EA+IVV+TLSGCGGDLY+VCAES+ + 
Sbjct: 1607 KIYKDLSAVQAQERKANYEMRTLKQKLRKSILKEAQIVVTTLSGCGGDLYSVCAESLAAH 1666

Query: 961  KFGSPSENTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVAS 1020
            KFGSPSE+ LFDAVVIDEAAQALEPATLIPLQLLKSR  +CIMVGDPKQLPATVLSNVAS
Sbjct: 1667 KFGSPSEDNLFDAVVIDEAAQALEPATLIPLQLLKSRGTKCIMVGDPKQLPATVLSNVAS 1726

Query: 1021 KFLYECSMFERLQRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIAPFHE 1080
            KFLYECSMFERLQRAG+P++MLT+QYRMHPEIC FPS HFYD KLLNG  MS K APFHE
Sbjct: 1727 KFLYECSMFERLQRAGYPILMLTQQYRMHPEICRFPSMHFYDNKLLNGVDMSSKSAPFHE 1786

Query: 1081 TKGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGII 1140
               LGPY+FYDIVDG+E R  SG + S+ NE EA+AAV+L++FFK+R+P+EF   RIGII
Sbjct: 1787 NHHLGPYVFYDIVDGQEHR--SGDSSSVCNEQEAEAAVQLLRFFKKRYPSEFVAGRIGII 1846

Query: 1141 TPYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINS 1200
            TPYK QL++LRS F+ +FGA +  DME NTVDGFQG+EVDIL+LSTVRA   S+  G+N 
Sbjct: 1847 TPYKRQLAVLRSRFTGAFGAQVTADMEMNTVDGFQGKEVDILVLSTVRATH-SAPDGVNQ 1906

Query: 1201 SGIGFVADARRMNVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDS 1260
            S IGFVAD RRMNVALTRAKLSLWVLGN+RTLQ + +WGAL+KDAKER +++ VK PY+ 
Sbjct: 1907 SRIGFVADVRRMNVALTRAKLSLWVLGNTRTLQRDHNWGALVKDAKEREVIIPVKRPYNY 1966

Query: 1261 IYKTAALRNSYQQTMDNNSRNLKHASRHAKRSRKETSESEGKDILSATQCTKTNDVDSSQ 1320
            ++    +  ++ + +  N           K  R ETS        S  +  KT+      
Sbjct: 1967 MFGENVMEQNHSENLPKNFPKPDKQHSRRKEQRAETS--------SDRKLRKTDG----- 2026

Query: 1321 YNASVKDALPLVA-GSINR-SLKVAKAAVHIEHGAEFGSKSGMSSEKKSNTGNISRGKRK 1380
                  D +P+ + GS ++ + + AK     +      S   ++SE+     +  + K K
Sbjct: 2027 ------DVVPISSKGSESKHTRRNAKEEASSQREKLVASCEKVTSEETLRRSHEKKEKMK 2086

Query: 1381 VDCGKSSNSDYAERGVVDNHALQISQTSKRLKESPWCDTICTNQEPSAPIIEGSSKEEHN 1440
                KSSN +  +     N      + S   K+S    +   + + + P  +   ++   
Sbjct: 2087 -GREKSSNPEITDANSSKN------ENSNEWKKSKKASSKLDSSKRANPTDKIGQQDRQI 2120

Query: 1441 NSTALSRSDTGKELIVKRKQQREAVDAILFSSLIPSKKSEMSMKLMSDKKPPFSNVRGCM 1491
            N    S     +++I KRKQQREAV AIL SSLIPS K +   + +S    P S      
Sbjct: 2147 NKGNASNQGGVEDMISKRKQQREAVAAILNSSLIPSHKPKPPKRPLS----PGSTAGSHT 2120

BLAST of Lag0003222 vs. TAIR 10
Match: AT2G19120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 3.4e-84
Identity = 267/858 (31.12%), Postives = 415/858 (48.37%), Query Frame = 0

Query: 423  LKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVDEFH 482
            L+ VP  F+S E+YV +F PL+ EE +AQL S++VE++  +      + + S+ER +   
Sbjct: 254  LRSVPERFESMEEYVRVFEPLLFEECRAQLYSTWVELAEANTYV--KVRIKSIERRE--- 313

Query: 483  LVRFAYD---DNNSVASKSFAENDLLLLTKELPQKS---SQGAHMVGKVDRRERDNKRKM 542
              R  YD   ++ +    +F E D+ +L+  LP+          + G V R    + R  
Sbjct: 314  --RGWYDVILNSLNGCKWAFKEGDVAVLSTPLPESDEDHEDAGRVAGTVRRHIPVDTRDP 373

Query: 543  NLLIIRFYLLNG---SSRLHQAR--KNLIERSKWHASRIMSITPQLREFQALSSIKDI-- 602
                + FY+ N     S++  +   + L  +  WH + + S+    RE+ AL +   +  
Sbjct: 374  RGATLHFYVGNSGGTGSKIDDSHILRKLKPQDIWHLTVLGSLATTQREYVALHAFSRLNP 433

Query: 603  PILPMILKPEANSIPC-DESKVVDLSKLSRPLQQILKSSFNVSQLQAI-----DISIGSR 662
             +   IL P     P  +E         +      L  SFN  QL AI       + G+ 
Sbjct: 434  QMQNAILNPSPEQFPSYEEQTPATPDCFTTSFVDHLHRSFNAPQLAAIHWAAMHTAAGTS 493

Query: 663  NMKNDLE---LSLVQGPPGTGKTRTILAIVSALLASASQRTNHATSLNRSLKQDNVLHAD 722
            +     E    +LVQGPPGTGKT T+  +++ +     Q   + TSL + L  +    A+
Sbjct: 494  SGVKKQEPWPFTLVQGPPGTGKTHTVWGMLNVI--HLVQYQQYYTSLLKKLAPETYNQAN 553

Query: 723  SRPQISQPVAIARAWQNAALARQLNEDKQRNSKPIDCTM-----KRRVLICAQSNAAVDE 782
                            +  L+  ++E  Q     +  T+     K R+L+CA SNAA DE
Sbjct: 554  ECSS-----------SDNILSGSIDEVLQNMDHNLFRTLPKLCAKPRMLVCAPSNAATDE 613

Query: 783  LVSRISNLGLYDSDGKMYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKN 842
            L+SR+ + G  D + ++Y+P + RVG   +    +    ++   DQ LA  R        
Sbjct: 614  LLSRVLDRGFIDGEMRVYRPDVARVG-VDSQSRAAQAVSVERRSDQLLAISRDEILRHMR 673

Query: 843  DLRTNSSMELRSNLEKLVDRIKYYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELEL 902
            +LR   + ++  N+  L   +        +      D     ++    D+K  +L +   
Sbjct: 674  NLRLQET-QISQNIAGLKRELNAAAFATRSQGSVGVD----PEVLISRDQKRDALLQ--- 733

Query: 903  KLRKLYEQKKQIYKDIS----IAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCG 962
             L  + E + ++  +IS    +   F    N  ++  +  L  S   EAEIV +T+S  G
Sbjct: 734  HLAAVVEARDKVLVEISRLLIVEGKFRAGNNFNLEEARASLEASFANEAEIVFTTVSSSG 793

Query: 963  GDLYAVCAESILSCKFGSPSENTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGD 1022
              L++                   FD VVIDEAAQA E   L PL L    A RC++VGD
Sbjct: 794  RKLFSRLTHG--------------FDMVVIDEAAQASEVGVLPPLAL---GAARCVLVGD 853

Query: 1023 PKQLPATVLSNVASKFLYECSMFERLQRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLL 1082
            P+QLPATV+S  A   LY  S+FER Q AG P ++LT QYRMHP+I  FPS++FY G+L 
Sbjct: 854  PQQLPATVISKAAGTLLYSRSLFERFQLAGCPTLLLTVQYRMHPQIRDFPSRYFYQGRLK 913

Query: 1083 NGDGMSGKIAP---FHETKGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVEL-VK 1142
            + + +S   AP   +++   L PY+F++I  G+E     GG+ S  N  EA   V + + 
Sbjct: 914  DSESISS--APDEIYYKDPVLRPYLFFNISHGRE--SHRGGSVSYENVDEARFCVGVYMH 973

Query: 1143 FFKERHPTEFSRVRIGIITPYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDIL 1202
              K        +V +G+ITPYK QL  L+  F  + G   + ++  NTVD FQG+E D++
Sbjct: 974  LQKTLKSLGAGKVSVGVITPYKLQLKCLKHEFGNALGQDELKEIYINTVDAFQGQERDVI 1033

Query: 1203 ILSTVRAADASSTSGINSSGIGFVADARRMNVALTRAKLSLWVLGNSRTLQVNPDWGALM 1245
            I+S VRA         +  G+GFV+D RRMNVALTRA+ +LWV+GN+  L  + DW AL+
Sbjct: 1034 IMSCVRA---------SGHGVGFVSDIRRMNVALTRARRALWVMGNASALMKSEDWAALI 1052

BLAST of Lag0003222 vs. TAIR 10
Match: AT4G30100.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 1.7e-83
Identity = 277/857 (32.32%), Postives = 427/857 (49.82%), Query Frame = 0

Query: 423  LKEVPVCFQSSEQYVEIFRPLILEEFKAQLRSSFVEMSSWDEMYLGSISVLSVERVDEFH 482
            L+ VP  F+S ++YV +F PL+ EE +AQL S++ E++  +  Y+  + +  +ER +   
Sbjct: 418  LQSVPERFESMDEYVRVFEPLLFEECRAQLYSTWEELAEANS-YM-KVRIKFIERRE--- 477

Query: 483  LVRFAYD---DNNSVASKSFAENDLLLLTKELPQKSSQGAH-----MVGKVDRRERDNKR 542
              R  YD   ++ +    +F E D+ +L+  +P+  S+G H     + G V R    + R
Sbjct: 478  --RGWYDVILNSVNECKWAFKEGDVAVLSNPVPE--SEGEHDDVGRVAGTVRRYIPVDTR 537

Query: 543  KMNLLIIRFYL---LNGSSRL---HQARKNLIERSKWHASRIMSITPQLREFQALSSIKD 602
              +  I+ FY+    +  S++   H  RK L  +  WH + + SI    RE+ AL +   
Sbjct: 538  DPHGAILHFYVGDAYDSGSKIDDNHILRK-LKPKEIWHLTVLGSIATTQREYVALHAFSQ 597

Query: 603  I--PILPMILKPEANSIPCDESKVVDLSKLSRP-LQQILKSSFNVSQLQAI-----DISI 662
            +   +   ILKP     P    +   +     P     L  SFN  QL AI       + 
Sbjct: 598  LNPQMQNAILKPSPEQFPNYGEQTPTVPDCFTPSFAGHLHRSFNAPQLAAIHWAAMHTAA 657

Query: 663  GSRN---MKNDLELSLVQGPPGTGKTRTILAIVSALLASASQRTNHATSLNRSL-----K 722
            G+ +    ++    +LVQGPPGTGKT T+  +++ +     Q   + TSL + L     K
Sbjct: 658  GTSSGVKRQDPWPFTLVQGPPGTGKTHTVWGMLNVI--HLVQYQQYYTSLLKKLAPESYK 717

Query: 723  QDNVLHADSRPQISQPVAIARAWQNAALARQLNEDKQRNSKPIDCTMKRRVLICAQSNAA 782
            Q N   +D+    S    +    QN  L R L         P  C  K R+L+CA SNAA
Sbjct: 718  QVNESSSDNIVSGSIDEVLQNMDQN--LFRTL---------PKLCA-KPRMLVCAPSNAA 777

Query: 783  VDELVSRISNLGLYDSDGKMYKPYLVRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSND 842
             DEL+SR+ + G  D + ++Y+P + RVG   T    +    ++   D  LA+ R     
Sbjct: 778  TDELLSRVLDRGFIDGEMRVYRPDVARVG-VDTQTKAAQAVSVERRSDLLLAKSREEILG 837

Query: 843  VKNDLRTNSSMELRSNLEKLVDRIKYYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKE 902
              ++LR   + +L  ++  L  + +      AN    +      V + +    ++  L+ 
Sbjct: 838  HIHNLRVRDA-QLSQDIAGL--KRELTAAAFANRSQGSVGVDPDVLMVR-DQTRDAMLQR 897

Query: 903  LELKLRKLYEQKKQIYKDISIAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGG 962
            L   +    +   ++ + + +   F   T+  ++  +  L  S   EAEIV +T+S  G 
Sbjct: 898  LSAVVEARDKDLVEMSRLLIVEGKFRAGTSFNLEEARASLEASFANEAEIVFTTVSSSGR 957

Query: 963  DLYAVCAESILSCKFGSPSENTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDP 1022
             L++                   FD VVIDEAAQA E   L PL L    A RC++VGDP
Sbjct: 958  KLFSRLTHG--------------FDMVVIDEAAQASEVGVLPPLAL---GAARCVLVGDP 1017

Query: 1023 KQLPATVLSNVASKFLYECSMFERLQRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLN 1082
            +QLPATV+S  A   LY  S+FER Q AG P ++LT QYRMHP+I  FPS++FY G+L +
Sbjct: 1018 QQLPATVISKAAGTLLYSRSLFERFQLAGCPTLLLTVQYRMHPQIRDFPSRYFYQGRLTD 1077

Query: 1083 GDGMSGKIAP---FHETKGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVEL-VKF 1142
             + +S   AP   +++   L PY+F+DI  G+E     GG+ S  N  EA   V + +  
Sbjct: 1078 SESVS--TAPDEIYYKDSVLKPYLFFDISHGRE--SHRGGSVSYENIDEARFCVGVYLHL 1137

Query: 1143 FKERHPTEFSRVRIGIITPYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDILI 1202
             +        +V +G+ITPYK QL  L+  F  +     + ++  NTVD FQG+E D++I
Sbjct: 1138 QRTLKSLGGGKVSVGVITPYKLQLKCLKIEFGNALSQDELQEIYINTVDAFQGQERDVII 1197

Query: 1203 LSTVRAADASSTSGINSSGIGFVADARRMNVALTRAKLSLWVLGNSRTLQVNPDWGALMK 1245
            +S VRA         ++ G+GFVAD RRMNVALTRAK +LWV+GN+  L    DW AL+ 
Sbjct: 1198 MSCVRA---------SNHGVGFVADIRRMNVALTRAKRALWVMGNASALMKCEDWAALIT 1215

BLAST of Lag0003222 vs. TAIR 10
Match: AT4G15570.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 1.5e-79
Identity = 263/892 (29.48%), Postives = 407/892 (45.63%), Query Frame = 0

Query: 394  WYRSILELDYFAMIGSTPVSEDKSQTVRHLKEVPVCFQSSEQYVEIFRPLILEEFKAQ-L 453
            +Y  IL  DY   +      +++  +   L  V   ++  + Y E F PL+ EE KAQ L
Sbjct: 19   FYNIILGWDY-KQLTKENERKNRKDSKEKLNVVKNTYKDVDDYFETFEPLLFEEVKAQIL 78

Query: 454  RSSFVEMSSWDEMYLGSISVLSVERVDEFHLVRFAYDDNNSVASKSFAENDLLLLTK-EL 513
            ++   E +S  +M L    V+     + FH +   Y+       +  A+NDLLLL+K E+
Sbjct: 79   QNKDGEEASVCKMRL----VMECNEGEGFHFLLVTYEHEE---DEYLAQNDLLLLSKEEV 138

Query: 514  PQKSSQGAHMVGKVDRRERDNKRKMNLLIIRFYLLNG---------SSRLH---QARKNL 573
               S   ++    V+ R+       NLL +R YL            SSR     QA  N+
Sbjct: 139  KGNSFPSSYGFAVVEHRQN------NLLRLRMYLAEDIVQITKNTKSSRTKSFIQALSNM 198

Query: 574  IERSKWHAS---------RIMSITPQLREFQALSSIKDIPILPMILKPEANSIPCDESKV 633
                   AS         ++  ++  +RE+ AL S+  +P   +I      S    +   
Sbjct: 199  RSLITSSASPIDKRVFSLKLCGLSTIIREYIALRSVSSLPFKDLIFTAAEKSCGFGD--- 258

Query: 634  VDLSKLSRPLQQILKSSFNVSQLQAIDISIGSRNMKNDLELSLVQGPPGTGKTRTILAIV 693
             +  K+S PL +    + N SQ +AID+ +  ++        L+QGPPGTGKT+TIL+I+
Sbjct: 259  -EAWKISGPLNEFFNENLNKSQKEAIDVGLSRKS------FVLIQGPPGTGKTQTILSIL 318

Query: 694  SALLASA-----SQRTNHATSLNRSLKQDNVLHADSRPQISQPVAIARAWQNAALARQLN 753
             A++ +      S+ T+H       +      +   R   + P  +    ++A +    +
Sbjct: 319  GAIMHATPARVQSKGTDHEVKRGIQMTIQEKYNHWGR---ASPWILGVNPRDAIMPEDGD 378

Query: 754  E------DKQRNSKPIDCTMK--RRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPYL 813
            +        +   + ++ + K   RVL+CA SN+A+DE+V R+ + GL D + + Y P +
Sbjct: 379  DGFFPTSGNELKPEVVNASRKYRLRVLVCAPSNSALDEIVLRLLSSGLRDENAQTYTPKI 438

Query: 814  VRVGNAKTVHPNSLPFFIDTLVDQQLAEERMNSNDVKNDLRTNSSMELRSNLEKLVDRIK 873
            VR+G     H +     +D LV    A++R ++ D      T + ++             
Sbjct: 439  VRIG--LKAHHSVASVSLDHLV----AQKRGSAIDKPKQGTTGTDID------------- 498

Query: 874  YYEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELKLRKLYEQKKQIYKDISIAQAF 933
                                                                        
Sbjct: 499  ------------------------------------------------------------ 558

Query: 934  EKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAESILSCKFGSPSENTLFD 993
                          +R +IL EA IV +TLS  G  L A                N  FD
Sbjct: 559  -------------SIRTAILEEAAIVFATLSFSGSALLA--------------KSNRGFD 618

Query: 994  AVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVLSNVASKFLYECSMFERL 1053
             V+IDEAAQA+EPATLIP   L +R  +  +VGDPKQLPATV+S VA    Y  SMFERL
Sbjct: 619  VVIIDEAAQAVEPATLIP---LATRCKQVFLVGDPKQLPATVISTVAQDSGYGTSMFERL 678

Query: 1054 QRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDGMSGKIA-PFHETKGLGPYIFYD 1113
            Q+AG+PV ML  QYRMHPEI  FPS+ FY+G L +G  +  +    +H+ +  GP+ F+D
Sbjct: 679  QKAGYPVKMLKTQYRMHPEIRSFPSKQFYEGALEDGSDIEAQTTRDWHKYRCFGPFCFFD 738

Query: 1114 IVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFSRVRIGIITPYKCQLSLLR 1173
            I +GKE +   G   S  N  E +  + +       +P   S  ++ II+PY  Q+   +
Sbjct: 739  IHEGKESQ-HPGATGSRVNLDEVEFVLLIYHRLVTMYPELKSSSQLAIISPYNYQVKTFK 764

Query: 1174 SCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADASSTSGINSSG-IGFVADAR 1233
              F   FG      ++ NTVDGFQGRE D+ I S VRA         N +G IGF++++R
Sbjct: 799  DRFKEMFGTEAEKVVDINTVDGFQGREKDVAIFSCVRA---------NENGQIGFLSNSR 764

Query: 1234 RMNVALTRAKLSLWVLGNSRTLQVNPDWGALMKDAKERNLVVSVKMPYDSIY 1248
            RMNV +TRAK S+ V+G++ TL+ +P W  L++ A++RN +  V  P ++ +
Sbjct: 859  RMNVGITRAKSSVLVVGSAATLKSDPLWKNLIESAEQRNRLFKVSKPLNNFF 764

BLAST of Lag0003222 vs. TAIR 10
Match: AT1G65810.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 5.1e-40
Identity = 159/538 (29.55%), Postives = 248/538 (46.10%), Query Frame = 0

Query: 739  MKRRVLICAQSNAAVDELVSRISNLGLYDSDGKMYKPY----LVRVGNAKTV----HPNS 798
            M+ R L CA +N AV E+ SR+  L    S+   +  Y    +V  GN + +      + 
Sbjct: 315  MRCRTLTCAPTNIAVLEVCSRLVKL---VSESLRFDGYGLGDIVLFGNKERMKIDDREDL 374

Query: 799  LPFFIDTLVDQ------QLAEERMNSN-------DVKNDLRTNSS-----MELRSNLEKL 858
               F++  VD+       L   R N N       D K++ R   S     +  +  +E+ 
Sbjct: 375  FDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHEFRQFKSVNTTLLSFKDFVEER 434

Query: 859  VDRIKY-----YEVKCANLRDENPDHKSSVQIFKGGDEKEMSLKELELK--LRKLYEQKK 918
            + R++Y     +   C +L    P    S ++ +  ++    L+ +     +R  Y + K
Sbjct: 435  LSRLRYDLHHQFTTLCLHL----PTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMK 494

Query: 919  QIYKDISIAQAFEKQTNEEVKALKHKLRKSILREAEIVVSTLSGCGGDLYAVCAES--IL 978
               KD       ++  +     L+     S+  +    +S       +L  +C ++  +L
Sbjct: 495  YKLKDTG-----DENDSRTQDCLEMLTSISMSIKLPDFISKF-----ELQKLCLDNAYLL 554

Query: 979  SCKFGSPSE---NTLFDAVVIDEAAQALEPATLIPLQLLKSRAIRCIMVGDPKQLPATVL 1038
             C   S +    ++    +VIDEAAQ  E  + IPLQL   +    I++GD KQLPA + 
Sbjct: 555  FCTASSSARLHMSSPIQLLVIDEAAQLKECESAIPLQLRGLQ--HAILIGDEKQLPAMIK 614

Query: 1039 SNVASKFLYECSMFERLQRAGHPVVMLTRQYRMHPEICHFPSQHFYDGKLLNGDG--MSG 1098
            SN+AS+     S+FERL   GH   +L  QYRMHP I  FP++ FYD K+L+     +  
Sbjct: 615  SNIASEADLGRSLFERLVLLGHNKQLLNMQYRMHPSISIFPNREFYDMKILDAPSVRLRS 674

Query: 1099 KIAPFHETKGLGPYIFYDIVDGKELRGKSGGAFSLYNEHEADAAVELVKFFKERHPTEFS 1158
                F   K  GPY F +I  G+E   + G  +S  N  E     E+V            
Sbjct: 675  YEKKFLPEKMYGPYSFINIAYGRE---QFGEGYSSKNLVEVSVVAEIVSKLYSVSRKTGR 734

Query: 1159 RVRIGIITPYKCQLSLLRSCFSRSFGASLIVDMEFNTVDGFQGREVDILILSTVRAADAS 1218
             + +G+I+PYK Q+  ++      +       +   +VDGFQG E DI+I+STVR+    
Sbjct: 735  TISVGVISPYKAQVFAIQERIGEKYNTEGTFTVSVRSVDGFQGGEEDIIIISTVRS---- 794

Query: 1219 STSGINSSG-IGFVADARRMNVALTRAKLSLWVLGNSRTLQVNPD-WGALMKDAKERN 1235
                 N +G IGF+++ +R NVALTRA+  LW+LGN  TL  N   W  L+ DAK RN
Sbjct: 795  -----NGNGAIGFLSNQQRTNVALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARN 821