Lag0003180 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003180 vs. NCBI nr
Match: XP_038897862.1 (subtilisin-like protease SBT1.5 [Benincasa hispida])

HSP 1 Score: 1480.7 bits (3832), Expect = 0.0e+00
Identity = 747/790 (94.56%), Postives = 766/790 (96.96%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASIS 60
           MAALL A+FLFSLLIP SSSSS+  D+SK TFIVQVH+D+KPSIFPTHKHWYESSL SIS
Sbjct: 1   MAALLPAIFLFSLLIPFSSSSST--DASKKTFIVQVHKDAKPSIFPTHKHWYESSLTSIS 60

Query: 61  PNKATTS----SSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPH 120
           PNKATTS    SS   G IIHTYE VFHGFSAKLSPFEVEKLQT PHIAS+IPEQVRHPH
Sbjct: 61  PNKATTSNPDASSTDCGAIIHTYETVFHGFSAKLSPFEVEKLQTLPHIASIIPEQVRHPH 120

Query: 121 TTRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQC 180
           TTRSPEFLGLKT+DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQC
Sbjct: 121 TTRSPEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQC 180

Query: 181 VTAKDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYV 240
           + AKDFP+TSCNRKLIGARFFC GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYV
Sbjct: 181 LEAKDFPATSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYV 240

Query: 241 FPASTLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGV 300
           FPASTLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGV
Sbjct: 241 FPASTLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGV 300

Query: 301 VVPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV 360
           VVPYYLDAIAI AFRAVA GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV
Sbjct: 301 VVPYYLDAIAIGAFRAVAGGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV 360

Query: 361 KLGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLC 420
           KLGNGRV+LGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGK+VLC
Sbjct: 361 KLGNGRVVLGTSVYGGPALVPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLC 420

Query: 421 DRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAE 480
           DRGINSRAAKGEVVKK+GGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAE
Sbjct: 421 DRGINSRAAKGEVVKKSGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAE 480

Query: 481 AAKSQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPD 540
           AAKS SPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWP+
Sbjct: 481 AAKSHSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPE 540

Query: 541 KIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLD 600
           KIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLD
Sbjct: 541 KIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLD 600

Query: 601 NRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQV 660
           NRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNY+TKNIQV
Sbjct: 601 NRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYSTKNIQV 660

Query: 661 ITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIK 720
           ITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGD+NSIYKV+IK
Sbjct: 661 ITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDSNSIYKVSIK 720

Query: 721 PPSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSP 780
           PPSGISVTVEPEKLAFRRVGQKL+FLVRV AMAVRLSPGSSSMKSGSIVWTDGKH VTSP
Sbjct: 721 PPSGISVTVEPEKLAFRRVGQKLNFLVRVQAMAVRLSPGSSSMKSGSIVWTDGKHEVTSP 780

Query: 781 LVVTMQQPLQ 787
           LVVTMQQPLQ
Sbjct: 781 LVVTMQQPLQ 788

BLAST of Lag0003180 vs. NCBI nr
Match: XP_023000250.1 (subtilisin-like protease SBT1.5 [Cucurbita maxima])

HSP 1 Score: 1471.8 bits (3809), Expect = 0.0e+00
Identity = 743/789 (94.17%), Postives = 763/789 (96.70%), Query Frame = 0

Query: 2   AALLRALFLFSLLIPL---SSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLAS 61
           AALLRAL LFSLL+PL   SSSSSSSAD+SK TFIVQVH DSKPSIFPTHKHWYESSLAS
Sbjct: 3   AALLRALVLFSLLLPLYSSSSSSSSSADASKKTFIVQVHGDSKPSIFPTHKHWYESSLAS 62

Query: 62  ISPNKAT-TSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHT 121
           I+ NKAT  +SS ++GPI+HTYENVFHGFSAKLSP EVEKLQT PHIAS+IPEQVRHPHT
Sbjct: 63  ITSNKATDATSSSSNGPILHTYENVFHGFSAKLSPSEVEKLQTLPHIASIIPEQVRHPHT 122

Query: 122 TRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCV 181
           TRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPS+WKGQCV
Sbjct: 123 TRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSEWKGQCV 182

Query: 182 TAKDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVF 241
            AKDFP+TSCNRKLIGARFFC GYEATNGKMNET+EYRSPRDSDGHGTHTASIAAGRYVF
Sbjct: 183 AAKDFPATSCNRKLIGARFFCSGYEATNGKMNETSEYRSPRDSDGHGTHTASIAAGRYVF 242

Query: 242 PASTLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVV 301
           PASTLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVV
Sbjct: 243 PASTLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVV 302

Query: 302 VPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVK 361
           VPYYLDAIAI AFRAV +GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVK
Sbjct: 303 VPYYLDAIAIGAFRAVEAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVK 362

Query: 362 LGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCD 421
           LGNGRVILGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPN V GK+VLCD
Sbjct: 363 LGNGRVILGTSVYGGPALVPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNSVNGKIVLCD 422

Query: 422 RGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEA 481
           RGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEA
Sbjct: 423 RGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGATGGDEIRKYIAEA 482

Query: 482 AKSQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDK 541
            KSQSPPTATILFKGTRLGVRPAPVVASFS+RGPNPESPEIVKPDVIAPGLNILAAWPDK
Sbjct: 483 VKSQSPPTATILFKGTRLGVRPAPVVASFSSRGPNPESPEIVKPDVIAPGLNILAAWPDK 542

Query: 542 IGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDN 601
           IGPSGIPTDKR  EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDN
Sbjct: 543 IGPSGIPTDKRAIEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDN 602

Query: 602 RGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVI 661
           RGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYT KNIQVI
Sbjct: 603 RGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTAKNIQVI 662

Query: 662 TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKP 721
           TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKP
Sbjct: 663 TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKP 722

Query: 722 PSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPL 781
           PSGISVTVEPE+LAFRRVGQKLSFLVRVHAMAV+LS GSSSMKSG +VWTDGKHVVTS L
Sbjct: 723 PSGISVTVEPERLAFRRVGQKLSFLVRVHAMAVKLSAGSSSMKSGYVVWTDGKHVVTSTL 782

Query: 782 VVTMQQPLQ 787
           VVTMQQPLQ
Sbjct: 783 VVTMQQPLQ 791

BLAST of Lag0003180 vs. NCBI nr
Match: KAA0059362.1 (subtilisin-like protease SBT1.5 [Cucumis melo var. makuwa] >TYK03964.1 subtilisin-like protease SBT1.5 [Cucumis melo var. makuwa])

HSP 1 Score: 1470.7 bits (3806), Expect = 0.0e+00
Identity = 743/787 (94.41%), Postives = 761/787 (96.70%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASIS 60
           MA LLRALFLFSLLIP   SSSSS D+SK TFIVQVH+DSKPSIFPTHKHWYESSLASI 
Sbjct: 1   MAVLLRALFLFSLLIPF--SSSSSIDASKKTFIVQVHKDSKPSIFPTHKHWYESSLASI- 60

Query: 61  PNKATTSSSIADG-PIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTR 120
                  SS+ DG  IIHTYE +FHGFSAKLSP EVEKLQTFPHIAS+IPEQVRHPHTTR
Sbjct: 61  -------SSVNDGAAIIHTYETLFHGFSAKLSPVEVEKLQTFPHIASIIPEQVRHPHTTR 120

Query: 121 SPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTA 180
           SPEFLGLKT+DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGP+PSKWKGQC+ A
Sbjct: 121 SPEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVA 180

Query: 181 KDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240
           KDFP++SCNRKLIGARFFC GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA
Sbjct: 181 KDFPASSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240

Query: 241 STLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP 300
           STLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP
Sbjct: 241 STLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP 300

Query: 301 YYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG 360
           YYLDAIAI A+RAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG
Sbjct: 301 YYLDAIAIGAYRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG 360

Query: 361 NGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRG 420
           NGRV+LGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGK+VLCDRG
Sbjct: 361 NGRVVLGTSVYGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRG 420

Query: 421 INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK 480
           INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK
Sbjct: 421 INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK 480

Query: 481 SQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG 540
           S SPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG
Sbjct: 481 SHSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG 540

Query: 541 PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG 600
           PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG
Sbjct: 541 PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG 600

Query: 601 ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITG 660
           ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITG
Sbjct: 601 ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITG 660

Query: 661 KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPS 720
           KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPPS
Sbjct: 661 KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPS 720

Query: 721 GISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVV 780
           GISVTVEPEKLAFRRVGQKLSFLVRV AMAVRLSPGSSSMKSGSI+WTDGKH VTSPLVV
Sbjct: 721 GISVTVEPEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVV 777

Query: 781 TMQQPLQ 787
           TMQQPLQ
Sbjct: 781 TMQQPLQ 777

BLAST of Lag0003180 vs. NCBI nr
Match: XP_023515158.1 (subtilisin-like protease SBT1.5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1470.3 bits (3805), Expect = 0.0e+00
Identity = 743/786 (94.53%), Postives = 761/786 (96.82%), Query Frame = 0

Query: 2   AALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISP 61
           AALLRAL LFSLL+PL  SSSSSAD+SK TFIVQVH DSKPSIFPTHKHWYESSLASI+ 
Sbjct: 3   AALLRALVLFSLLLPL-YSSSSSADASKKTFIVQVHGDSKPSIFPTHKHWYESSLASITS 62

Query: 62  NKAT-TSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 121
           NKAT  +SS ++GPI+HTYENVFHGFSAKLSP EVEKLQT PHIAS+IPEQVRHPHTTRS
Sbjct: 63  NKATYATSSSSNGPILHTYENVFHGFSAKLSPSEVEKLQTLPHIASIIPEQVRHPHTTRS 122

Query: 122 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 181
           PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCV AK
Sbjct: 123 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVAAK 182

Query: 182 DFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 241
           DFP+TSCNRKLIGARFFC GYEATNGKMNET+EYRSPRDSDGHGTHTASIAAGRYVFPAS
Sbjct: 183 DFPATSCNRKLIGARFFCSGYEATNGKMNETSEYRSPRDSDGHGTHTASIAAGRYVFPAS 242

Query: 242 TLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 301
           TLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY
Sbjct: 243 TLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 302

Query: 302 YLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 361
           YLDAIAI AFRAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN
Sbjct: 303 YLDAIAIGAFRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 362

Query: 362 GRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGI 421
           GRVILGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPN V GK+VLCDRGI
Sbjct: 363 GRVILGTSVYGGPALVPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNSVNGKIVLCDRGI 422

Query: 422 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKS 481
           NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEA KS
Sbjct: 423 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGATGGDEIRKYIAEAVKS 482

Query: 482 QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 541
           QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP
Sbjct: 483 QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 542

Query: 542 SGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 601
           SGIPTDKR  EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE
Sbjct: 543 SGIPTDKRAIEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 602

Query: 602 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGK 661
           TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYT KNIQVITGK
Sbjct: 603 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTAKNIQVITGK 662

Query: 662 IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSG 721
           IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPPSG
Sbjct: 663 IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSG 722

Query: 722 ISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVT 781
           ISVTVEPE+LAFRRVGQKLSFLVRV AMAV+LS GSSSMKSG +VWTDGKHVVTS LVVT
Sbjct: 723 ISVTVEPERLAFRRVGQKLSFLVRVQAMAVKLSAGSSSMKSGYVVWTDGKHVVTSTLVVT 782

Query: 782 MQQPLQ 787
           MQQPLQ
Sbjct: 783 MQQPLQ 787

BLAST of Lag0003180 vs. NCBI nr
Match: KAG6593579.1 (Subtilisin-like protease 1.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1470.3 bits (3805), Expect = 0.0e+00
Identity = 743/786 (94.53%), Postives = 761/786 (96.82%), Query Frame = 0

Query: 2   AALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISP 61
           AALLRAL LFSLL+PL  SSSSSAD+SK TFIVQVH DSKPSIFPTHKHWYESSLASI+ 
Sbjct: 3   AALLRALVLFSLLLPL-YSSSSSADASKKTFIVQVHGDSKPSIFPTHKHWYESSLASITS 62

Query: 62  NKAT-TSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 121
           NKAT  +SS ++GPI+HTYENVFHGFSAKLSP EVEKLQT PHIAS+IPEQVRHPHTTRS
Sbjct: 63  NKATDATSSSSNGPILHTYENVFHGFSAKLSPSEVEKLQTLPHIASIIPEQVRHPHTTRS 122

Query: 122 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 181
           PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCV AK
Sbjct: 123 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVAAK 182

Query: 182 DFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 241
           DFP+TSCNRKLIGARFFC GYEATNGKMNET+EYRSPRDSDGHGTHTASIAAGRYVFPAS
Sbjct: 183 DFPATSCNRKLIGARFFCSGYEATNGKMNETSEYRSPRDSDGHGTHTASIAAGRYVFPAS 242

Query: 242 TLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 301
           TLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY
Sbjct: 243 TLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 302

Query: 302 YLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 361
           YLDAIAI AFRAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN
Sbjct: 303 YLDAIAIGAFRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 362

Query: 362 GRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGI 421
           GRVILGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPN V GK+VLCDRGI
Sbjct: 363 GRVILGTSVYGGPALVPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNSVNGKIVLCDRGI 422

Query: 422 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKS 481
           NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEA KS
Sbjct: 423 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGATGGDEIRKYIAEAVKS 482

Query: 482 QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 541
           QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP
Sbjct: 483 QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 542

Query: 542 SGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 601
           SGIPTDKR  EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE
Sbjct: 543 SGIPTDKRAIEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 602

Query: 602 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGK 661
           TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYT KNIQVITGK
Sbjct: 603 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTAKNIQVITGK 662

Query: 662 IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSG 721
           IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPPSG
Sbjct: 663 IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSG 722

Query: 722 ISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVT 781
           ISVTVEPE+LAFRRVGQKLSFLVRV AMAV+LS GSSSMKSG +VWTDGKHVVTS LVVT
Sbjct: 723 ISVTVEPERLAFRRVGQKLSFLVRVQAMAVKLSAGSSSMKSGYVVWTDGKHVVTSTLVVT 782

Query: 782 MQQPLQ 787
           MQQPLQ
Sbjct: 783 MQQPLQ 787

BLAST of Lag0003180 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 593/780 (76.03%), Postives = 691/780 (88.59%), Query Frame = 0

Query: 9   FLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISPNKATTSS 68
           F F  L+ LSS SSS++ S+  T+IV V  ++KPSIFPTH HWY SSLAS++ +  +   
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS--- 64

Query: 69  SIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRSPEFLGLKT 128
                 IIHTY+ VFHGFSA+L+  +  +L   PH+ SVIPEQVRH HTTRSPEFLGL++
Sbjct: 65  ------IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS 124

Query: 129 TDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAKDFPSTSCN 188
           TD AGLL+ESDFGSDLVIGVIDTG+WPER SF+DR LGP+P KWKGQC+ ++DFP ++CN
Sbjct: 125 TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACN 184

Query: 189 RKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGR 248
           RKL+GARFFCGGYEATNGKMNETTE+RSPRDSDGHGTHTASI+AGRYVFPASTLGYA G 
Sbjct: 185 RKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGV 244

Query: 249 AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIA 308
           AAGMAPKARLAAYKVCWN+GCYDSDILAAFD AV+DGVDV+SLSVGGVVVPYYLDAIAI 
Sbjct: 245 AAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIG 304

Query: 309 AFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVILGTS 368
           AF A+  G+FVSASAGNGGPG LTVTNVAPW+TTVGAGT+DRDFPA+VKLGNG++I G S
Sbjct: 305 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 364

Query: 369 VYGGPALAPGRLYPLIYAGT-EGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGINSRAAKG 428
           VYGGP L PGR+YPL+Y G+  GGDGYSSSLCLEGSL+PNLVKGK+VLCDRGINSRA KG
Sbjct: 365 VYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKG 424

Query: 429 EVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSQSP--PT 488
           E+V+K GGLGMI+ANGVFDGEGLVADCHVLPAT+VGASGGDEIR+YI+E++KS+S   PT
Sbjct: 425 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 484

Query: 489 ATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPT 548
           ATI+FKGTRLG+RPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPD+IGPSG+ +
Sbjct: 485 ATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTS 544

Query: 549 DKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDE 608
           D R TEFNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SAL+TTAYT+DN GE M+DE
Sbjct: 545 DNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDE 604

Query: 609 SSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCS 668
           S+GNTS+V+D+G+GHVHP KAMDPGL+YD+ +YDY++FLCNSNYT  NI  IT + ADC 
Sbjct: 605 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 664

Query: 669 GAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSGISVTV 728
           GA+RAGH GNLNYPS SVVFQQYG+ KMSTHFIR+VTNVGD++S+Y++ I+PP G +VTV
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 724

Query: 729 EPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVTMQQPL 786
           EPEKL+FRRVGQKLSF+VRV    V+LSPG++++++G IVW+DGK  VTSPLVVT+QQPL
Sbjct: 725 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Lag0003180 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 5.7e-229
Identity = 418/791 (52.84%), Postives = 550/791 (69.53%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASIS 60
           MA+    L LF L  P  S ++S A     TFI ++   S PSIFPTH HWY +  A   
Sbjct: 1   MASSTIVLLLF-LSFPFISFAASQA---AKTFIFRIDGGSMPSIFPTHYHWYSTEFAE-- 60

Query: 61  PNKATTSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 120
                      +  I+H Y  VFHGFSA ++P E + L+  P + +V  ++ R  HTTRS
Sbjct: 61  -----------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRS 120

Query: 121 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 180
           P+FLGL+  +  GL  ESD+GSD++IGV DTGIWPER+SF+D +LGPIP +W+G C +  
Sbjct: 121 PQFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGA 180

Query: 181 DFPSTSCNRKLIGARFFCGGYE-ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240
            F   +CNRK+IGARFF  G + A  G +N+T E+ SPRD+DGHGTHT+S AAGR+ F A
Sbjct: 181 RFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKA 240

Query: 241 STLGYARGRAAGMAPKARLAAYKVCW-NAGCYDSDILAAFDAAVSDGVDVVSLSVG---G 300
           S  GYA G A G+APKAR+AAYKVCW ++GC DSDILAAFDAAV DGVDV+S+S+G   G
Sbjct: 241 SMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDG 300

Query: 301 VVVPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPAD 360
           +  PYYLD IAI ++ A + G+FVS+SAGN GP G++VTN+APWVTTVGA T+DR+FPAD
Sbjct: 301 ITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPAD 360

Query: 361 VKLGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVL 420
             LG+G  + G S+Y G  L  GR++P++Y G  G    S+SLC+E +L+P  V+GK+V+
Sbjct: 361 AILGDGHRLRGVSLYAGVPL-NGRMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVI 420

Query: 421 CDRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIA 480
           CDRG + R AKG VVKKAGG+GMILANG  +GEGLV D H++PA AVG++ GD I+ Y  
Sbjct: 421 CDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAY-- 480

Query: 481 EAAKSQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWP 540
             A S   P A+I F+GT +G++PAPV+ASFS RGPN  SPEI+KPD+IAPG+NILAAW 
Sbjct: 481 --ASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWT 540

Query: 541 DKIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTL 600
           D +GP+G+P+D R TEFNILSGTSMACPHVSG AALLK+AHP WSPA I+SA+MTT   +
Sbjct: 541 DAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLV 600

Query: 601 DNRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQ 660
           DN   +++DES+G ++T  D+G+GH++  +AM+PGL+YD+   DY+ FLC+  Y  K IQ
Sbjct: 601 DNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQ 660

Query: 661 VITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTI 720
           VIT     C   ++    GNLNYPS++ VF    +  +S   IR+ TNVG A ++Y+  I
Sbjct: 661 VITRTPVRCPTTRKPS-PGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 720

Query: 721 KPPSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTD-GKHVVT 780
           + P G++VTV+P +L F    ++ S+ V V      +  G +    GS+ W D GKHVV 
Sbjct: 721 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVR 764

Query: 781 SPLVVTMQQPL 786
           SP+VVT    L
Sbjct: 781 SPIVVTQMDTL 764

BLAST of Lag0003180 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 3.2e-211
Identity = 394/770 (51.17%), Postives = 513/770 (66.62%), Query Frame = 0

Query: 11  FSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISPNKATTSSSI 70
           F LL+ L     SS+ S + T+IV + +   PS F  H +WY+SSL SIS +        
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS-------- 70

Query: 71  ADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRSPEFLGLKTTD 130
               +++TYEN  HGFS +L+  E + L T P + SV+PE     HTTR+P FLGL    
Sbjct: 71  --AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL-DEH 130

Query: 131 SAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAKDFPSTSCNRK 190
           +A L  E+   SD+V+GV+DTG+WPE +S++D   GPIPS WKG C    +F ++ CNRK
Sbjct: 131 TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRK 190

Query: 191 LIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGRAA 250
           LIGARFF  GYE+T G ++E+ E RSPRD DGHGTHT+S AAG  V  AS LGYA G A 
Sbjct: 191 LIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 250

Query: 251 GMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIAAF 310
           GMAP+AR+A YKVCW  GC+ SDILAA D A++D V+V+S+S+GG +  YY D +AI AF
Sbjct: 251 GMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAF 310

Query: 311 RAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVILGTSVY 370
            A+  G+ VS SAGN GP   +++NVAPW+TTVGAGT+DRDFPA   LGNG+   G S++
Sbjct: 311 AAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF 370

Query: 371 GGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGINSRAAKGEVV 430
            G AL P +L P IYAG    +  + +LC+ G+L P  VKGK+V+CDRGIN+R  KG+VV
Sbjct: 371 KGEAL-PDKLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 430

Query: 431 KKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSQSPPTATILF 490
           K AGG+GMILAN   +GE LVAD H+LPAT VG   GD IR Y+     +   PTA+I  
Sbjct: 431 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT----TDPNPTASISI 490

Query: 491 KGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTT 550
            GT +GV+P+PVVA+FS+RGPN  +P I+KPD+IAPG+NILAAW    GP+G+ +D R  
Sbjct: 491 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 550

Query: 551 EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNT 610
           EFNI+SGTSM+CPHVSGLAALLK+ HP WSPAAI+SALMTTAY     G+ +LD ++G  
Sbjct: 551 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 610

Query: 611 STVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRA 670
           ST  D GAGHV P  A +PGLIYDL T DY+ FLC  NYT+  I+ ++ +   C  +K +
Sbjct: 611 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK-S 670

Query: 671 GHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSGISVTVEPEKL 730
               +LNYPS +V     G +K    + R+VT+VG A +         +G+ ++VEP  L
Sbjct: 671 YSVADLNYPSFAVNVDGVGAYK----YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 730

Query: 731 AFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVT 781
            F+   +K S+ V      V  S  S S   GSI W+DGKHVV SP+ ++
Sbjct: 731 NFKEANEKKSYTV---TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Lag0003180 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 9.2e-203
Identity = 397/792 (50.13%), Postives = 511/792 (64.52%), Query Frame = 0

Query: 3   ALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASI--S 62
           +L    F+F LL+   S SSSS+D  + ++IV V R  KPS+F +H +W+ S L S+  S
Sbjct: 5   SLSSIFFVFPLLLCFFSPSSSSSDGLE-SYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSS 64

Query: 63  PNKATTSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 122
           P  AT         ++++Y    HGFSA+LSP +   L+  P + SVIP+Q R  HTT +
Sbjct: 65  PQPAT---------LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHT 124

Query: 123 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 182
           P FLG   + ++GL   S++G D+++GV+DTGIWPE  SF+D  LGPIPS WKG+C    
Sbjct: 125 PAFLGF--SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGP 184

Query: 183 DFPSTSCNRKLIGARFFCGGY--EATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFP 242
           DFP++SCNRKLIGAR F  GY  +    K +   E RSPRD++GHGTHTAS AAG  V  
Sbjct: 185 DFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVAN 244

Query: 243 ASTLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVG--GV 302
           AS   YARG A GMA KAR+AAYK+CW  GCYDSDILAA D AV+DGV V+SLSVG  G 
Sbjct: 245 ASLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGS 304

Query: 303 VVPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV 362
              Y+ D+IAI AF A   G+ VS SAGN GP   T TN+APW+ TVGA T+DR+F A+ 
Sbjct: 305 APEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANA 364

Query: 363 KLGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLC 422
             G+G+V  GTS+Y G +L   +L  L+Y+G  G     S LC  G LN +LV+GK+VLC
Sbjct: 365 ITGDGKVFTGTSLYAGESLPDSQL-SLVYSGDCG-----SRLCYPGKLNSSLVEGKIVLC 424

Query: 423 DRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAE 482
           DRG N+R  KG  VK AGG GMILAN    GE L AD H++PAT VGA  GD+IR YI  
Sbjct: 425 DRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYI-- 484

Query: 483 AAKSQSPPTATILFKGTRLGVR-PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWP 542
             K+   PTA I F GT +G   P+P VA+FS+RGPN  +P I+KPDVIAPG+NILA W 
Sbjct: 485 --KTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWT 544

Query: 543 DKIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTL 602
             +GP+ +  D R  +FNI+SGTSM+CPHVSGLAALL+ AHP WSPAAIKSAL+TTAY +
Sbjct: 545 GMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDV 604

Query: 603 DNRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQ 662
           +N GE + D ++G +S     GAGHV P KA++PGL+YD+   +YV FLC   Y    I 
Sbjct: 605 ENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGIL 664

Query: 663 VI--TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVG-DANSIYK 722
           V      + D     +    G+LNYPS SVVF   G+      + R V NVG + +++Y+
Sbjct: 665 VFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGE---VVKYKRVVKNVGSNVDAVYE 724

Query: 723 VTIKPPSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGS-SSMKSGSIVWTDGKH 782
           V +K P+ + + V P KLAF +    L + V   ++ +    GS    + GSI WTDG+H
Sbjct: 725 VGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEH 771

Query: 783 VVTSPLVVTMQQ 784
           VV SP+ V   Q
Sbjct: 785 VVKSPVAVQWGQ 771

BLAST of Lag0003180 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 1.6e-202
Identity = 389/776 (50.13%), Postives = 508/776 (65.46%), Query Frame = 0

Query: 18  SSSSSSSA-------------DSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISPNKA 77
           SSSSSSS+              ++K T+I++V+   KP  F TH  WY S L S      
Sbjct: 3   SSSSSSSSITIITTFLFLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------ 62

Query: 78  TTSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPH-IASVIPEQVRHPHTTRSPEF 137
                  +  +++TY   FHGFSA L   E + L +  + I  +  + +   HTTR+PEF
Sbjct: 63  -------ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEF 122

Query: 138 LGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAKDFP 197
           LGL +      L  S  G  ++IGV+DTG+WPE +SF+D D+  IPSKWKG+C +  DF 
Sbjct: 123 LGLNSEFGVHDLGSSSNG--VIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFD 182

Query: 198 STSCNRKLIGARFFCGGYE-ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTL 257
           S  CN+KLIGAR F  G++ A+ G  +   E  SPRD DGHGTHT++ AAG  V  AS L
Sbjct: 183 SKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFL 242

Query: 258 GYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYL 317
           GYA G A GMA +AR+A YKVCW+ GC+ SDILAA D A+ DGVDV+SLS+GG   PYY 
Sbjct: 243 GYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYR 302

Query: 318 DAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGR 377
           D IAI AF A+  GVFVS SAGN GP   +V NVAPWV TVGAGT+DRDFPA   LGNG+
Sbjct: 303 DTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGK 362

Query: 378 VILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGINS 437
            + G S+Y G  +    L  L+Y     G+  SS+LCL GSL+ ++V+GK+V+CDRG+N+
Sbjct: 363 RLTGVSLYSGVGMGTKPL-ELVY---NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNA 422

Query: 438 RAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSQS 497
           R  KG VV+ AGGLGMI+AN    GE LVAD H+LPA AVG   GD +R+Y+    KS S
Sbjct: 423 RVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYV----KSDS 482

Query: 498 PPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSG 557
            PTA ++FKGT L V+P+PVVA+FS+RGPN  +PEI+KPDVI PG+NILA W D IGP+G
Sbjct: 483 KPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTG 542

Query: 558 IPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETM 617
           +  D R T+FNI+SGTSM+CPH+SGLA LLKAAHP WSP+AIKSALMTTAY LDN    +
Sbjct: 543 LDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPL 602

Query: 618 LDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIA 677
            D +  + S     G+GHV PQKA+ PGL+YD++T +Y+ FLC+ +YT  +I  I  K  
Sbjct: 603 HDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIV-KRP 662

Query: 678 DCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSGIS 737
             + +K+    G LNYPS SV+F      K    + R VTNVG A+S+YKVT+     + 
Sbjct: 663 SVNCSKKFSDPGQLNYPSFSVLF----GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVG 722

Query: 738 VTVEPEKLAFRRVGQKLSFLVR-VHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPL 778
           ++V+P KL+F+ VG+K  + V  V    V +   ++  + GSI W++ +H V SP+
Sbjct: 723 ISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM---TNKAEFGSITWSNPQHEVRSPV 747

BLAST of Lag0003180 vs. ExPASy TrEMBL
Match: A0A6J1KHT9 (subtilisin-like protease SBT1.5 OS=Cucurbita maxima OX=3661 GN=LOC111494531 PE=3 SV=1)

HSP 1 Score: 1471.8 bits (3809), Expect = 0.0e+00
Identity = 743/789 (94.17%), Postives = 763/789 (96.70%), Query Frame = 0

Query: 2   AALLRALFLFSLLIPL---SSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLAS 61
           AALLRAL LFSLL+PL   SSSSSSSAD+SK TFIVQVH DSKPSIFPTHKHWYESSLAS
Sbjct: 3   AALLRALVLFSLLLPLYSSSSSSSSSADASKKTFIVQVHGDSKPSIFPTHKHWYESSLAS 62

Query: 62  ISPNKAT-TSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHT 121
           I+ NKAT  +SS ++GPI+HTYENVFHGFSAKLSP EVEKLQT PHIAS+IPEQVRHPHT
Sbjct: 63  ITSNKATDATSSSSNGPILHTYENVFHGFSAKLSPSEVEKLQTLPHIASIIPEQVRHPHT 122

Query: 122 TRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCV 181
           TRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPS+WKGQCV
Sbjct: 123 TRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSEWKGQCV 182

Query: 182 TAKDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVF 241
            AKDFP+TSCNRKLIGARFFC GYEATNGKMNET+EYRSPRDSDGHGTHTASIAAGRYVF
Sbjct: 183 AAKDFPATSCNRKLIGARFFCSGYEATNGKMNETSEYRSPRDSDGHGTHTASIAAGRYVF 242

Query: 242 PASTLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVV 301
           PASTLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVV
Sbjct: 243 PASTLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVV 302

Query: 302 VPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVK 361
           VPYYLDAIAI AFRAV +GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVK
Sbjct: 303 VPYYLDAIAIGAFRAVEAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVK 362

Query: 362 LGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCD 421
           LGNGRVILGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPN V GK+VLCD
Sbjct: 363 LGNGRVILGTSVYGGPALVPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNSVNGKIVLCD 422

Query: 422 RGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEA 481
           RGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEA
Sbjct: 423 RGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGATGGDEIRKYIAEA 482

Query: 482 AKSQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDK 541
            KSQSPPTATILFKGTRLGVRPAPVVASFS+RGPNPESPEIVKPDVIAPGLNILAAWPDK
Sbjct: 483 VKSQSPPTATILFKGTRLGVRPAPVVASFSSRGPNPESPEIVKPDVIAPGLNILAAWPDK 542

Query: 542 IGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDN 601
           IGPSGIPTDKR  EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDN
Sbjct: 543 IGPSGIPTDKRAIEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDN 602

Query: 602 RGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVI 661
           RGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYT KNIQVI
Sbjct: 603 RGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTAKNIQVI 662

Query: 662 TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKP 721
           TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKP
Sbjct: 663 TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKP 722

Query: 722 PSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPL 781
           PSGISVTVEPE+LAFRRVGQKLSFLVRVHAMAV+LS GSSSMKSG +VWTDGKHVVTS L
Sbjct: 723 PSGISVTVEPERLAFRRVGQKLSFLVRVHAMAVKLSAGSSSMKSGYVVWTDGKHVVTSTL 782

Query: 782 VVTMQQPLQ 787
           VVTMQQPLQ
Sbjct: 783 VVTMQQPLQ 791

BLAST of Lag0003180 vs. ExPASy TrEMBL
Match: A0A5D3BYA5 (Subtilisin-like protease SBT1.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001610 PE=3 SV=1)

HSP 1 Score: 1470.7 bits (3806), Expect = 0.0e+00
Identity = 743/787 (94.41%), Postives = 761/787 (96.70%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASIS 60
           MA LLRALFLFSLLIP   SSSSS D+SK TFIVQVH+DSKPSIFPTHKHWYESSLASI 
Sbjct: 1   MAVLLRALFLFSLLIPF--SSSSSIDASKKTFIVQVHKDSKPSIFPTHKHWYESSLASI- 60

Query: 61  PNKATTSSSIADG-PIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTR 120
                  SS+ DG  IIHTYE +FHGFSAKLSP EVEKLQTFPHIAS+IPEQVRHPHTTR
Sbjct: 61  -------SSVNDGAAIIHTYETLFHGFSAKLSPVEVEKLQTFPHIASIIPEQVRHPHTTR 120

Query: 121 SPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTA 180
           SPEFLGLKT+DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGP+PSKWKGQC+ A
Sbjct: 121 SPEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVA 180

Query: 181 KDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240
           KDFP++SCNRKLIGARFFC GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA
Sbjct: 181 KDFPASSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240

Query: 241 STLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP 300
           STLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP
Sbjct: 241 STLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP 300

Query: 301 YYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG 360
           YYLDAIAI A+RAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG
Sbjct: 301 YYLDAIAIGAYRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG 360

Query: 361 NGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRG 420
           NGRV+LGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGK+VLCDRG
Sbjct: 361 NGRVVLGTSVYGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRG 420

Query: 421 INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK 480
           INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK
Sbjct: 421 INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK 480

Query: 481 SQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG 540
           S SPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG
Sbjct: 481 SHSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG 540

Query: 541 PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG 600
           PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG
Sbjct: 541 PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG 600

Query: 601 ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITG 660
           ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITG
Sbjct: 601 ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITG 660

Query: 661 KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPS 720
           KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPPS
Sbjct: 661 KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPS 720

Query: 721 GISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVV 780
           GISVTVEPEKLAFRRVGQKLSFLVRV AMAVRLSPGSSSMKSGSI+WTDGKH VTSPLVV
Sbjct: 721 GISVTVEPEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVV 777

Query: 781 TMQQPLQ 787
           TMQQPLQ
Sbjct: 781 TMQQPLQ 777

BLAST of Lag0003180 vs. ExPASy TrEMBL
Match: A0A6J1HJN0 (subtilisin-like protease SBT1.5 OS=Cucurbita moschata OX=3662 GN=LOC111464184 PE=3 SV=1)

HSP 1 Score: 1469.9 bits (3804), Expect = 0.0e+00
Identity = 742/786 (94.40%), Postives = 761/786 (96.82%), Query Frame = 0

Query: 2   AALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISP 61
           AALLRAL LFSLL+PL  SSSSSAD+SK TFIVQVH DSKPSIFPTHKHWYESSLASI+ 
Sbjct: 3   AALLRALVLFSLLLPL-YSSSSSADASKKTFIVQVHGDSKPSIFPTHKHWYESSLASITS 62

Query: 62  NKAT-TSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 121
           NKAT  +SS ++GPI+HTYENVFHGFSAKLSP EVEKLQT PHIAS+IPEQVRHPHTTRS
Sbjct: 63  NKATDATSSSSNGPILHTYENVFHGFSAKLSPSEVEKLQTLPHIASIIPEQVRHPHTTRS 122

Query: 122 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 181
           PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCV AK
Sbjct: 123 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVAAK 182

Query: 182 DFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 241
           DFP+TSCNRKLIGARFFC GYEATNGKMNET+EYRSPRDSDGHGTHTASIAAGRYVFPAS
Sbjct: 183 DFPATSCNRKLIGARFFCSGYEATNGKMNETSEYRSPRDSDGHGTHTASIAAGRYVFPAS 242

Query: 242 TLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 301
           TLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY
Sbjct: 243 TLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 302

Query: 302 YLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 361
           YLDAIAI AFRAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN
Sbjct: 303 YLDAIAIGAFRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 362

Query: 362 GRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGI 421
           GR+ILGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPN V GK+VLCDRGI
Sbjct: 363 GRIILGTSVYGGPALVPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNSVNGKIVLCDRGI 422

Query: 422 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKS 481
           NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEA KS
Sbjct: 423 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGATGGDEIRKYIAEAVKS 482

Query: 482 QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 541
           QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP
Sbjct: 483 QSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 542

Query: 542 SGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 601
           SGIPTDKR  EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE
Sbjct: 543 SGIPTDKRAIEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 602

Query: 602 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGK 661
           TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYT KNIQVITGK
Sbjct: 603 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTAKNIQVITGK 662

Query: 662 IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSG 721
           IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPPSG
Sbjct: 663 IADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSG 722

Query: 722 ISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVT 781
           ISVTVEPE+LAFRRVGQKLSFLVRV AMAV+LS GSSSMKSG +VWTDGKHVVTS LVVT
Sbjct: 723 ISVTVEPERLAFRRVGQKLSFLVRVQAMAVKLSAGSSSMKSGYVVWTDGKHVVTSTLVVT 782

Query: 782 MQQPLQ 787
           MQQPLQ
Sbjct: 783 MQQPLQ 787

BLAST of Lag0003180 vs. ExPASy TrEMBL
Match: A0A6J1JY93 (subtilisin-like protease SBT1.5 OS=Cucurbita maxima OX=3661 GN=LOC111488559 PE=3 SV=1)

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 741/790 (93.80%), Postives = 765/790 (96.84%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRD-SKPSIFPTHKHWYESSLASI 60
           MAALLR LFLFSLL+PL   SSSS D+SK TFIVQV+RD SKPSIFPTH+HWYESSLASI
Sbjct: 1   MAALLRGLFLFSLLLPL---SSSSIDNSKKTFIVQVNRDASKPSIFPTHRHWYESSLASI 60

Query: 61  SPNKATTS---SSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPH 120
           SPNK T S   +S +DGPIIHTYE +FHGFSAKLSPFEV+ LQT PHIA+VIPEQVRHPH
Sbjct: 61  SPNKPTPSQPDASGSDGPIIHTYETLFHGFSAKLSPFEVQNLQTLPHIAAVIPEQVRHPH 120

Query: 121 TTRSPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQC 180
           TTRSPEFLGL T+DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKG+C
Sbjct: 121 TTRSPEFLGLTTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGRC 180

Query: 181 VTAKDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYV 240
           + +K FP+TSCNRKLIGARFFC GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYV
Sbjct: 181 LESKHFPATSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYV 240

Query: 241 FPASTLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGV 300
           FPASTLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGV
Sbjct: 241 FPASTLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGV 300

Query: 301 VVPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV 360
           VVPYYLDAIAI AFRAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV
Sbjct: 301 VVPYYLDAIAIGAFRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV 360

Query: 361 KLGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLC 420
           KLGNGRVILGTSVYGGPAL PGR++PLIYAGTEGGDGYSSSLCLEGSLNPNLVKGK+V+C
Sbjct: 361 KLGNGRVILGTSVYGGPALVPGRMHPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVVC 420

Query: 421 DRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAE 480
           DRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAE
Sbjct: 421 DRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGAAGGDEIRKYIAE 480

Query: 481 AAKSQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPD 540
           AAKS+SPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPD
Sbjct: 481 AAKSKSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPD 540

Query: 541 KIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLD 600
           KIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLD
Sbjct: 541 KIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLD 600

Query: 601 NRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQV 660
           NRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQV
Sbjct: 601 NRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQV 660

Query: 661 ITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIK 720
           ITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIK
Sbjct: 661 ITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIK 720

Query: 721 PPSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSP 780
           PP GISVTVEPEKLAFRRVGQKLSFLVRV AMA+RLS GSSSMKSGSIVWTDGKHVVTSP
Sbjct: 721 PPRGISVTVEPEKLAFRRVGQKLSFLVRVQAMALRLSSGSSSMKSGSIVWTDGKHVVTSP 780

Query: 781 LVVTMQQPLQ 787
           LVVTMQQPLQ
Sbjct: 781 LVVTMQQPLQ 787

BLAST of Lag0003180 vs. ExPASy TrEMBL
Match: A0A1S3CI11 (subtilisin-like protease SBT1.5 OS=Cucumis melo OX=3656 GN=LOC103500650 PE=3 SV=1)

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 740/787 (94.03%), Postives = 758/787 (96.32%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASIS 60
           MA LLRA FLFSLLIP   SSSSS D+SK TFIVQVH+DSKPSIFPTHKHWYESSLASI 
Sbjct: 1   MAVLLRAFFLFSLLIPF--SSSSSIDASKKTFIVQVHKDSKPSIFPTHKHWYESSLASI- 60

Query: 61  PNKATTSSSIAD-GPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTR 120
                  SS+ D G IIHTYE +FHGFSAKLSP EVEKLQT PHIAS+IPEQVRHPHTTR
Sbjct: 61  -------SSVNDGGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHIASIIPEQVRHPHTTR 120

Query: 121 SPEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTA 180
           SPEFLGLKT+DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGP+PSKWKGQC+ A
Sbjct: 121 SPEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVA 180

Query: 181 KDFPSTSCNRKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240
           KDFP++SCNRKLIGARFFC GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA
Sbjct: 181 KDFPASSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240

Query: 241 STLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP 300
           STLGYARG+AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP
Sbjct: 241 STLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVP 300

Query: 301 YYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG 360
           YYLDAIAI A+RAVA+GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG
Sbjct: 301 YYLDAIAIGAYRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLG 360

Query: 361 NGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRG 420
           NGRV+LGTSVYGGPAL PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGK+VLCDRG
Sbjct: 361 NGRVVLGTSVYGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRG 420

Query: 421 INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK 480
           INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK
Sbjct: 421 INSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAK 480

Query: 481 SQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG 540
           S SPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG
Sbjct: 481 SHSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIG 540

Query: 541 PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG 600
           PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG
Sbjct: 541 PSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRG 600

Query: 601 ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITG 660
           ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITG
Sbjct: 601 ETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITG 660

Query: 661 KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPS 720
           KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPPS
Sbjct: 661 KIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPS 720

Query: 721 GISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVV 780
           GISVTVEPEKLAFRRVGQKLSFLVRV AMAVRLSPGSSSMK GSI+WTDGKH VTSPLVV
Sbjct: 721 GISVTVEPEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKGGSIIWTDGKHEVTSPLVV 777

Query: 781 TMQQPLQ 787
           TMQQPLQ
Sbjct: 781 TMQQPLQ 777

BLAST of Lag0003180 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 593/780 (76.03%), Postives = 691/780 (88.59%), Query Frame = 0

Query: 9   FLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISPNKATTSS 68
           F F  L+ LSS SSS++ S+  T+IV V  ++KPSIFPTH HWY SSLAS++ +  +   
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS--- 64

Query: 69  SIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRSPEFLGLKT 128
                 IIHTY+ VFHGFSA+L+  +  +L   PH+ SVIPEQVRH HTTRSPEFLGL++
Sbjct: 65  ------IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS 124

Query: 129 TDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAKDFPSTSCN 188
           TD AGLL+ESDFGSDLVIGVIDTG+WPER SF+DR LGP+P KWKGQC+ ++DFP ++CN
Sbjct: 125 TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACN 184

Query: 189 RKLIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGR 248
           RKL+GARFFCGGYEATNGKMNETTE+RSPRDSDGHGTHTASI+AGRYVFPASTLGYA G 
Sbjct: 185 RKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGV 244

Query: 249 AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIA 308
           AAGMAPKARLAAYKVCWN+GCYDSDILAAFD AV+DGVDV+SLSVGGVVVPYYLDAIAI 
Sbjct: 245 AAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIG 304

Query: 309 AFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVILGTS 368
           AF A+  G+FVSASAGNGGPG LTVTNVAPW+TTVGAGT+DRDFPA+VKLGNG++I G S
Sbjct: 305 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 364

Query: 369 VYGGPALAPGRLYPLIYAGT-EGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGINSRAAKG 428
           VYGGP L PGR+YPL+Y G+  GGDGYSSSLCLEGSL+PNLVKGK+VLCDRGINSRA KG
Sbjct: 365 VYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKG 424

Query: 429 EVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSQSP--PT 488
           E+V+K GGLGMI+ANGVFDGEGLVADCHVLPAT+VGASGGDEIR+YI+E++KS+S   PT
Sbjct: 425 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 484

Query: 489 ATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPT 548
           ATI+FKGTRLG+RPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPD+IGPSG+ +
Sbjct: 485 ATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTS 544

Query: 549 DKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDE 608
           D R TEFNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SAL+TTAYT+DN GE M+DE
Sbjct: 545 DNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDE 604

Query: 609 SSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCS 668
           S+GNTS+V+D+G+GHVHP KAMDPGL+YD+ +YDY++FLCNSNYT  NI  IT + ADC 
Sbjct: 605 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 664

Query: 669 GAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSGISVTV 728
           GA+RAGH GNLNYPS SVVFQQYG+ KMSTHFIR+VTNVGD++S+Y++ I+PP G +VTV
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 724

Query: 729 EPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVTMQQPL 786
           EPEKL+FRRVGQKLSF+VRV    V+LSPG++++++G IVW+DGK  VTSPLVVT+QQPL
Sbjct: 725 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Lag0003180 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 795.4 bits (2053), Expect = 4.1e-230
Identity = 418/791 (52.84%), Postives = 550/791 (69.53%), Query Frame = 0

Query: 1   MAALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASIS 60
           MA+    L LF L  P  S ++S A     TFI ++   S PSIFPTH HWY +  A   
Sbjct: 1   MASSTIVLLLF-LSFPFISFAASQA---AKTFIFRIDGGSMPSIFPTHYHWYSTEFAE-- 60

Query: 61  PNKATTSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 120
                      +  I+H Y  VFHGFSA ++P E + L+  P + +V  ++ R  HTTRS
Sbjct: 61  -----------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRS 120

Query: 121 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 180
           P+FLGL+  +  GL  ESD+GSD++IGV DTGIWPER+SF+D +LGPIP +W+G C +  
Sbjct: 121 PQFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGA 180

Query: 181 DFPSTSCNRKLIGARFFCGGYE-ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPA 240
            F   +CNRK+IGARFF  G + A  G +N+T E+ SPRD+DGHGTHT+S AAGR+ F A
Sbjct: 181 RFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKA 240

Query: 241 STLGYARGRAAGMAPKARLAAYKVCW-NAGCYDSDILAAFDAAVSDGVDVVSLSVG---G 300
           S  GYA G A G+APKAR+AAYKVCW ++GC DSDILAAFDAAV DGVDV+S+S+G   G
Sbjct: 241 SMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDG 300

Query: 301 VVVPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPAD 360
           +  PYYLD IAI ++ A + G+FVS+SAGN GP G++VTN+APWVTTVGA T+DR+FPAD
Sbjct: 301 ITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPAD 360

Query: 361 VKLGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVL 420
             LG+G  + G S+Y G  L  GR++P++Y G  G    S+SLC+E +L+P  V+GK+V+
Sbjct: 361 AILGDGHRLRGVSLYAGVPL-NGRMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVI 420

Query: 421 CDRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIA 480
           CDRG + R AKG VVKKAGG+GMILANG  +GEGLV D H++PA AVG++ GD I+ Y  
Sbjct: 421 CDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAY-- 480

Query: 481 EAAKSQSPPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWP 540
             A S   P A+I F+GT +G++PAPV+ASFS RGPN  SPEI+KPD+IAPG+NILAAW 
Sbjct: 481 --ASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWT 540

Query: 541 DKIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTL 600
           D +GP+G+P+D R TEFNILSGTSMACPHVSG AALLK+AHP WSPA I+SA+MTT   +
Sbjct: 541 DAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLV 600

Query: 601 DNRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQ 660
           DN   +++DES+G ++T  D+G+GH++  +AM+PGL+YD+   DY+ FLC+  Y  K IQ
Sbjct: 601 DNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQ 660

Query: 661 VITGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTI 720
           VIT     C   ++    GNLNYPS++ VF    +  +S   IR+ TNVG A ++Y+  I
Sbjct: 661 VITRTPVRCPTTRKPS-PGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARI 720

Query: 721 KPPSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTD-GKHVVT 780
           + P G++VTV+P +L F    ++ S+ V V      +  G +    GS+ W D GKHVV 
Sbjct: 721 ESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVR 764

Query: 781 SPLVVTMQQPL 786
           SP+VVT    L
Sbjct: 781 SPIVVTQMDTL 764

BLAST of Lag0003180 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 736.5 bits (1900), Expect = 2.2e-212
Identity = 394/770 (51.17%), Postives = 513/770 (66.62%), Query Frame = 0

Query: 11  FSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISPNKATTSSSI 70
           F LL+ L     SS+ S + T+IV + +   PS F  H +WY+SSL SIS +        
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS-------- 70

Query: 71  ADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRSPEFLGLKTTD 130
               +++TYEN  HGFS +L+  E + L T P + SV+PE     HTTR+P FLGL    
Sbjct: 71  --AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL-DEH 130

Query: 131 SAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAKDFPSTSCNRK 190
           +A L  E+   SD+V+GV+DTG+WPE +S++D   GPIPS WKG C    +F ++ CNRK
Sbjct: 131 TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRK 190

Query: 191 LIGARFFCGGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGRAA 250
           LIGARFF  GYE+T G ++E+ E RSPRD DGHGTHT+S AAG  V  AS LGYA G A 
Sbjct: 191 LIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 250

Query: 251 GMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIAAF 310
           GMAP+AR+A YKVCW  GC+ SDILAA D A++D V+V+S+S+GG +  YY D +AI AF
Sbjct: 251 GMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAF 310

Query: 311 RAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVILGTSVY 370
            A+  G+ VS SAGN GP   +++NVAPW+TTVGAGT+DRDFPA   LGNG+   G S++
Sbjct: 311 AAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF 370

Query: 371 GGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGINSRAAKGEVV 430
            G AL P +L P IYAG    +  + +LC+ G+L P  VKGK+V+CDRGIN+R  KG+VV
Sbjct: 371 KGEAL-PDKLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 430

Query: 431 KKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSQSPPTATILF 490
           K AGG+GMILAN   +GE LVAD H+LPAT VG   GD IR Y+     +   PTA+I  
Sbjct: 431 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT----TDPNPTASISI 490

Query: 491 KGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTT 550
            GT +GV+P+PVVA+FS+RGPN  +P I+KPD+IAPG+NILAAW    GP+G+ +D R  
Sbjct: 491 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 550

Query: 551 EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNT 610
           EFNI+SGTSM+CPHVSGLAALLK+ HP WSPAAI+SALMTTAY     G+ +LD ++G  
Sbjct: 551 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 610

Query: 611 STVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRA 670
           ST  D GAGHV P  A +PGLIYDL T DY+ FLC  NYT+  I+ ++ +   C  +K +
Sbjct: 611 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK-S 670

Query: 671 GHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSGISVTVEPEKL 730
               +LNYPS +V     G +K    + R+VT+VG A +         +G+ ++VEP  L
Sbjct: 671 YSVADLNYPSFAVNVDGVGAYK----YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 730

Query: 731 AFRRVGQKLSFLVRVHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPLVVT 781
            F+   +K S+ V      V  S  S S   GSI W+DGKHVV SP+ ++
Sbjct: 731 NFKEANEKKSYTV---TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Lag0003180 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 708.4 bits (1827), Expect = 6.6e-204
Identity = 397/792 (50.13%), Postives = 511/792 (64.52%), Query Frame = 0

Query: 3   ALLRALFLFSLLIPLSSSSSSSADSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASI--S 62
           +L    F+F LL+   S SSSS+D  + ++IV V R  KPS+F +H +W+ S L S+  S
Sbjct: 5   SLSSIFFVFPLLLCFFSPSSSSSDGLE-SYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSS 64

Query: 63  PNKATTSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPHIASVIPEQVRHPHTTRS 122
           P  AT         ++++Y    HGFSA+LSP +   L+  P + SVIP+Q R  HTT +
Sbjct: 65  PQPAT---------LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHT 124

Query: 123 PEFLGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAK 182
           P FLG   + ++GL   S++G D+++GV+DTGIWPE  SF+D  LGPIPS WKG+C    
Sbjct: 125 PAFLGF--SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGP 184

Query: 183 DFPSTSCNRKLIGARFFCGGY--EATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFP 242
           DFP++SCNRKLIGAR F  GY  +    K +   E RSPRD++GHGTHTAS AAG  V  
Sbjct: 185 DFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVAN 244

Query: 243 ASTLGYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVG--GV 302
           AS   YARG A GMA KAR+AAYK+CW  GCYDSDILAA D AV+DGV V+SLSVG  G 
Sbjct: 245 ASLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGS 304

Query: 303 VVPYYLDAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADV 362
              Y+ D+IAI AF A   G+ VS SAGN GP   T TN+APW+ TVGA T+DR+F A+ 
Sbjct: 305 APEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANA 364

Query: 363 KLGNGRVILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLC 422
             G+G+V  GTS+Y G +L   +L  L+Y+G  G     S LC  G LN +LV+GK+VLC
Sbjct: 365 ITGDGKVFTGTSLYAGESLPDSQL-SLVYSGDCG-----SRLCYPGKLNSSLVEGKIVLC 424

Query: 423 DRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAE 482
           DRG N+R  KG  VK AGG GMILAN    GE L AD H++PAT VGA  GD+IR YI  
Sbjct: 425 DRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYI-- 484

Query: 483 AAKSQSPPTATILFKGTRLGVR-PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWP 542
             K+   PTA I F GT +G   P+P VA+FS+RGPN  +P I+KPDVIAPG+NILA W 
Sbjct: 485 --KTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWT 544

Query: 543 DKIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTL 602
             +GP+ +  D R  +FNI+SGTSM+CPHVSGLAALL+ AHP WSPAAIKSAL+TTAY +
Sbjct: 545 GMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDV 604

Query: 603 DNRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQ 662
           +N GE + D ++G +S     GAGHV P KA++PGL+YD+   +YV FLC   Y    I 
Sbjct: 605 ENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGIL 664

Query: 663 VI--TGKIADCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVG-DANSIYK 722
           V      + D     +    G+LNYPS SVVF   G+      + R V NVG + +++Y+
Sbjct: 665 VFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGE---VVKYKRVVKNVGSNVDAVYE 724

Query: 723 VTIKPPSGISVTVEPEKLAFRRVGQKLSFLVRVHAMAVRLSPGS-SSMKSGSIVWTDGKH 782
           V +K P+ + + V P KLAF +    L + V   ++ +    GS    + GSI WTDG+H
Sbjct: 725 VGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEH 771

Query: 783 VVTSPLVVTMQQ 784
           VV SP+ V   Q
Sbjct: 785 VVKSPVAVQWGQ 771

BLAST of Lag0003180 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 707.6 bits (1825), Expect = 1.1e-203
Identity = 389/776 (50.13%), Postives = 508/776 (65.46%), Query Frame = 0

Query: 18  SSSSSSSA-------------DSSKNTFIVQVHRDSKPSIFPTHKHWYESSLASISPNKA 77
           SSSSSSS+              ++K T+I++V+   KP  F TH  WY S L S      
Sbjct: 3   SSSSSSSSITIITTFLFLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------ 62

Query: 78  TTSSSIADGPIIHTYENVFHGFSAKLSPFEVEKLQTFPH-IASVIPEQVRHPHTTRSPEF 137
                  +  +++TY   FHGFSA L   E + L +  + I  +  + +   HTTR+PEF
Sbjct: 63  -------ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEF 122

Query: 138 LGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTAKDFP 197
           LGL +      L  S  G  ++IGV+DTG+WPE +SF+D D+  IPSKWKG+C +  DF 
Sbjct: 123 LGLNSEFGVHDLGSSSNG--VIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFD 182

Query: 198 STSCNRKLIGARFFCGGYE-ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTL 257
           S  CN+KLIGAR F  G++ A+ G  +   E  SPRD DGHGTHT++ AAG  V  AS L
Sbjct: 183 SKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFL 242

Query: 258 GYARGRAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYL 317
           GYA G A GMA +AR+A YKVCW+ GC+ SDILAA D A+ DGVDV+SLS+GG   PYY 
Sbjct: 243 GYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYR 302

Query: 318 DAIAIAAFRAVASGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGR 377
           D IAI AF A+  GVFVS SAGN GP   +V NVAPWV TVGAGT+DRDFPA   LGNG+
Sbjct: 303 DTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGK 362

Query: 378 VILGTSVYGGPALAPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKLVLCDRGINS 437
            + G S+Y G  +    L  L+Y     G+  SS+LCL GSL+ ++V+GK+V+CDRG+N+
Sbjct: 363 RLTGVSLYSGVGMGTKPL-ELVY---NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNA 422

Query: 438 RAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSQS 497
           R  KG VV+ AGGLGMI+AN    GE LVAD H+LPA AVG   GD +R+Y+    KS S
Sbjct: 423 RVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYV----KSDS 482

Query: 498 PPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSG 557
            PTA ++FKGT L V+P+PVVA+FS+RGPN  +PEI+KPDVI PG+NILA W D IGP+G
Sbjct: 483 KPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTG 542

Query: 558 IPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETM 617
           +  D R T+FNI+SGTSM+CPH+SGLA LLKAAHP WSP+AIKSALMTTAY LDN    +
Sbjct: 543 LDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPL 602

Query: 618 LDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIA 677
            D +  + S     G+GHV PQKA+ PGL+YD++T +Y+ FLC+ +YT  +I  I  K  
Sbjct: 603 HDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIV-KRP 662

Query: 678 DCSGAKRAGHTGNLNYPSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPSGIS 737
             + +K+    G LNYPS SV+F      K    + R VTNVG A+S+YKVT+     + 
Sbjct: 663 SVNCSKKFSDPGQLNYPSFSVLF----GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVG 722

Query: 738 VTVEPEKLAFRRVGQKLSFLVR-VHAMAVRLSPGSSSMKSGSIVWTDGKHVVTSPL 778
           ++V+P KL+F+ VG+K  + V  V    V +   ++  + GSI W++ +H V SP+
Sbjct: 723 ISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM---TNKAEFGSITWSNPQHEVRSPV 747