Lag0003137 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003137 vs. NCBI nr
Match: XP_023000031.1 (nodulation-signaling pathway 1 protein [Cucurbita maxima])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.6e-291
Identity = 505/555 (90.99%), Postives = 528/555 (95.14%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFF-SPFLEETYNSSSINCYQWWDENQDMGQDLINGC 60
           MTIEEPG NHPSDHILDWL DSVPFF SPF +++YNSSSINCYQWWDENQD+GQDLINGC
Sbjct: 40  MTIEEPGANHPSDHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGC 99

Query: 61  LSSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKN 120
           LSSSP   TTVST+PPNT TSHHLTPSDLTKKRKAPD+TVHK SQ+ QNQRKNQNNQSKN
Sbjct: 100 LSSSP---TTVSTKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKN 159

Query: 121 GADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDA 180
           GADKG GAVEGVTVIKKSVGNK+NSSK+TGNN +NGSNKEGRWAEQLLNPCANAIIKGDA
Sbjct: 160 GADKGSGAVEGVTVIKKSVGNKRNSSKATGNNNHNGSNKEGRWAEQLLNPCANAIIKGDA 219

Query: 181 TRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDP 240
           TRVHHLLCVLQELASP GDANHRLAA+GLRALAH+LSSN SS  SSSSTVAPVTFASTD 
Sbjct: 220 TRVHHLLCVLQELASPTGDANHRLAAYGLRALAHYLSSN-SSCYSSSSTVAPVTFASTDL 279

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEP+RSRNLHILDIGVSHGVQWPTLLE
Sbjct: 280 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLE 339

Query: 301 ALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTVIAPTVEHDQN ETPFSIGPPGDNISSRLLSFAKSLNINLQINR
Sbjct: 340 ALTRRSGGPPPLIRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 399

Query: 361 LDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSE 420
           LDN SLQS NSQVI K PDEILIVCAQFRLHQL+HYAPDERFEFLQNLRK++PKAVILSE
Sbjct: 400 LDNRSLQSFNSQVIGKLPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSE 459

Query: 421 NNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEM 480
           NNMACSC+NCGNFDTGFTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAA+ L NQGEM
Sbjct: 460 NNMACSCNNCGNFDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAARGLTNQGEM 519

Query: 481 NEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEE EKWCERM+NAGFARK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPV
Sbjct: 520 NEEMEKWCERMRNAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPV 579

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLGMKSNGG
Sbjct: 580 SFCSFWKLGMKSNGG 590

BLAST of Lag0003137 vs. NCBI nr
Match: XP_023514155.1 (nodulation-signaling pathway 1 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 987.6 bits (2552), Expect = 4.2e-284
Identity = 492/543 (90.61%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLEETYNSSSINCYQWWDENQDMGQDLINGCLSSSPTTVTTVS 72
           DHILDWL DSVPFF SPF +++YNSSSINCYQWWDENQD+GQDLINGCLSSSP   TTVS
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNGADKGGGAVEGV 132
           T+PPN  TSHHLTPSDLTKKRKAPD+TVHK SQ+ QNQRKNQNNQSKNGADK  GAV GV
Sbjct: 104 TKPPNNSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKNGADKSSGAVAGV 163

Query: 133 TVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KKSVGNK+NSSK+TG+N NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGSNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASP GDANHRLAA+GLRALAH+LSSN SS SSSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSN-SSCSSSSSTLAPVTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILHILSEEP+RSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQSLNSQ 372
           IRLTVIAPT+EHDQN ETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDN SLQSLNSQ
Sbjct: 344 IRLTVIAPTLEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNRSLQSLNSQ 403

Query: 373 VIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSENNMACSCSNCGN 432
           VI KFPDEILIVCAQFRLHQL+HYAPDERFEFLQNLRKM+PKAVILSENNMACSC+NCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKMEPKAVILSENNMACSCNNCGN 463

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEMNEEKEKWCERMK 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAAKAL N+GEMNEE EKWCERM+
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGFARK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPVSFCSFWKLG+KS
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGIKS 582

Query: 553 NGG 555
           NGG
Sbjct: 584 NGG 582

BLAST of Lag0003137 vs. NCBI nr
Match: XP_038897214.1 (protein NODULATION SIGNALING PATHWAY 1 [Benincasa hispida])

HSP 1 Score: 987.3 bits (2551), Expect = 5.5e-284
Identity = 497/554 (89.71%), Postives = 519/554 (93.68%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLEETYNSSSINCYQWWDENQDMGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDSVPFFSPFL+ET NSSSINCYQWWD NQD G+DLING L
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSPFLDETNNSSSINCYQWWDANQDTGEDLINGFL 60

Query: 61  SSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNG 120
           S+SP   TTVST+  N PTSHHLTPSDLTKKRKAPD++VHK SQ+HQN+RKNQNNQSKNG
Sbjct: 61  SNSP---TTVSTKLQNIPTSHHLTPSDLTKKRKAPDDSVHKKSQTHQNRRKNQNNQSKNG 120

Query: 121 ADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
              GGGAVEGVTV+KKSVGNKKNSSK TGNN NNGSN+EGRWAEQLLNPCA+AIIKGDAT
Sbjct: 121 ---GGGAVEGVTVMKKSVGNKKNSSKFTGNNCNNGSNREGRWAEQLLNPCASAIIKGDAT 180

Query: 181 RVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSST--SSSSSTVAPVTFASTD 240
           RVHHLLCVLQELASP GDANHRLA HGLRALAHHLSSNSSS+  SS SSTVAPVTFASTD
Sbjct: 181 RVHHLLCVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSFSSYSSTVAPVTFASTD 240

Query: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLL 300
           PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEP+R RNLHILDIGVSHGVQWPTLL
Sbjct: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRPRNLHILDIGVSHGVQWPTLL 300

Query: 301 EALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360
           EALTRRSGGPP LIRLTVI PT+EHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN
Sbjct: 301 EALTRRSGGPPSLIRLTVIGPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360

Query: 361 RLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILS 420
           RL+NHSLQSLNSQVI KFPDEILIVCAQFRLHQL+H  PDERFEFLQNLRKM+PKAVILS
Sbjct: 361 RLNNHSLQSLNSQVINKFPDEILIVCAQFRLHQLKHCDPDERFEFLQNLRKMEPKAVILS 420

Query: 421 ENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGE 480
           ENNM CSCSNCGNFDT FTRRVEYLWRFLDSTS+AFKGRES+ERRVMEGEAAKAL N GE
Sbjct: 421 ENNMGCSCSNCGNFDTRFTRRVEYLWRFLDSTSAAFKGRESEERRVMEGEAAKALTNDGE 480

Query: 481 MNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQP 540
           MNEEK KWCERM+NAGF RK FAEDTIDTARASMRRYDNNWEMRIEEKDGC+GLWWKGQP
Sbjct: 481 MNEEKGKWCERMRNAGFERKLFAEDTIDTARASMRRYDNNWEMRIEEKDGCLGLWWKGQP 540

Query: 541 VSFCSFWKLGMKSN 553
           VSFCSFWKLG+KSN
Sbjct: 541 VSFCSFWKLGIKSN 548

BLAST of Lag0003137 vs. NCBI nr
Match: XP_022964298.1 (nodulation-signaling pathway 1 protein [Cucurbita moschata])

HSP 1 Score: 981.5 bits (2536), Expect = 3.0e-282
Identity = 489/543 (90.06%), Postives = 518/543 (95.40%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLEETYNSSSINCYQWWDENQDMGQDLINGCLSSSPTTVTTVS 72
           DHILDWL DSVPFF SPF +++YNSSSINCYQWWDENQD+GQDLINGCLSSSP   TTVS
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNGADKGGGAVEGV 132
           T+PPNT TSHHLTPSDLTKKRKAPD+TVHK SQ+ QNQRKNQNNQS+NGADK  GAV GV
Sbjct: 104 TKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSRNGADKSSGAVAGV 163

Query: 133 TVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KKSVGNK+NSSK+TGNN NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGNNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASP GDANHRLAA+GLRALAH+LSSNSS   SSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSNSS--CSSSSTLAPVTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILHILSEEP+RSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQSLNSQ 372
           IRLTVIAPTVEHDQN ETPFSIGPPGDNISSRLLSFAKSLNINLQINRLD+ SL SLN+Q
Sbjct: 344 IRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDSLSLLSLNAQ 403

Query: 373 VIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSENNMACSCSNCGN 432
           VI KFPDEILIVCAQFRLHQL+HYAPDERFEFLQNLRK++PKAVILSENNMACSC+NCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSENNMACSCTNCGN 463

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEMNEEKEKWCERMK 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER++MEGEAAKAL N+GEMNEE EKWCERM+
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKLMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGFARK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPVSFCSFWKLGMKS
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGMKS 581

Query: 553 NGG 555
           NGG
Sbjct: 584 NGG 581

BLAST of Lag0003137 vs. NCBI nr
Match: KAG6593549.1 (Protein NODULATION SIGNALING PATHWAY 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 981.1 bits (2535), Expect = 4.0e-282
Identity = 488/543 (89.87%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLEETYNSSSINCYQWWDENQDMGQDLINGCLSSSPTTVTTVS 72
           DHILDWL DSVPFF SPF +++YNS+SINCYQWWDENQD+GQDLINGCLSSSP   TTVS
Sbjct: 5   DHILDWLADSVPFFSSPFPDDSYNSNSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 64

Query: 73  TRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNGADKGGGAVEGV 132
           T+PPNT TSHHLTPSDLTKKRKAPD+TVHK SQ+ QNQRKNQNNQS+NGADK  GAV GV
Sbjct: 65  TKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSRNGADKSSGAVAGV 124

Query: 133 TVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KKSVGNK+NSSK+TGNN NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 125 TVMKKSVGNKRNSSKATGNNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 184

Query: 193 LASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASP GDANHRLAA+GLRALAH+LSSN SS SSSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 185 LASPTGDANHRLAAYGLRALAHYLSSN-SSCSSSSSTLAPVTFASTDPRFFQRSLIKFHE 244

Query: 253 VSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILHILSEEP+RSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 245 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 304

Query: 313 IRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQSLNSQ 372
           IRLTVIAPTVEHDQN ETPFSIGPPGDNISSRLLSFAKSLNINLQINRLD+ SL SLN+Q
Sbjct: 305 IRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDSLSLLSLNAQ 364

Query: 373 VIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSENNMACSCSNCGN 432
           VI KFPDEILIVCAQFRLHQL+HYAPDERFEFLQNLRK++PKAVILSENNMACSC+NCGN
Sbjct: 365 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSENNMACSCTNCGN 424

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEMNEEKEKWCERMK 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER++MEGEAAKAL N+GEMNEE EKWCERM+
Sbjct: 425 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKLMEGEAAKALRNEGEMNEEMEKWCERMR 484

Query: 493 NAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGFARK F EDTIDTARASMRRYDNNWEMR++EKDGCVGLWWKGQPVSFCSFWKLGMKS
Sbjct: 485 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVDEKDGCVGLWWKGQPVSFCSFWKLGMKS 543

Query: 553 NGG 555
           NGG
Sbjct: 545 NGG 543

BLAST of Lag0003137 vs. ExPASy Swiss-Prot
Match: Q4VYC8 (Protein NODULATION SIGNALING PATHWAY 1 OS=Medicago truncatula OX=3880 GN=NSP1 PE=1 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.6e-185
Identity = 348/560 (62.14%), Postives = 423/560 (75.54%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLEETYNSSSINCYQWWDENQDM-GQDLINGC 60
           MT+E   PN  SDHILDWLE SV FF  FL++ YN+  I+ Y+ W++NQD+  Q  I+  
Sbjct: 1   MTME---PNPTSDHILDWLEGSVSFFPSFLDDPYNNGYIHEYEIWNQNQDISNQYQIDAN 60

Query: 61  LSSSPTTVTTVSTRPPNTPTS---------HHLTPSDLTKKRKAPDE-TVHKTSQSHQNQ 120
            +SS  T +T +    +T TS         +++  SDL KKR A DE ++ K  Q+ +N+
Sbjct: 61  TNSSNATNSTTNIVAASTTTSTTSLEPNSFNNIPFSDLPKKRNAEDELSLKKQPQNQKNK 120

Query: 121 RKNQNNQSKNGADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNP 180
           R    ++  N +D G  A+EG TV++KS GNKK ++K+ G+N NNG+NK+GRWAEQLLNP
Sbjct: 121 R--LKSRPMNESDNGDAALEG-TVVRKSGGNKKGAAKANGSNSNNGNNKDGRWAEQLLNP 180

Query: 181 CANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTV 240
           CA AI  G+  RV HLL VL ELAS  GDANHRLAAHGLRAL HHLSS+SSST S +   
Sbjct: 181 CAVAITGGNLNRVQHLLYVLHELASTTGDANHRLAAHGLRALTHHLSSSSSSTPSGT--- 240

Query: 241 APVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVS 300
             +TFAST+PRFFQ+SL+KF+E SPWF+FPNNIAN+SIL +L+EEP+  R LHILDIGVS
Sbjct: 241 --ITFASTEPRFFQKSLLKFYEFSPWFSFPNNIANASILQVLAEEPNNLRTLHILDIGVS 300

Query: 301 HGVQWPTLLEALTRRSGGPPPLIRLTVI--APTVEHDQNTETPFSIGPPGDNISSRLLSF 360
           HGVQWPT LEAL+RR GGPPPL+RLTV+  + + E+DQN ETPFSIGP GD  SS LL +
Sbjct: 301 HGVQWPTFLEALSRRPGGPPPLVRLTVVNASSSTENDQNMETPFSIGPCGDTFSSGLLGY 360

Query: 361 AKSLNINLQINRLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNL 420
           A+SLN+NLQI +LDNH LQ+LN++ +    DE LIVCAQFRLH L H  PDER EFL+ L
Sbjct: 361 AQSLNVNLQIKKLDNHPLQTLNAKSVDTSSDETLIVCAQFRLHHLNHNNPDERSEFLKVL 420

Query: 421 RKMDPKAVILSENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEG 480
           R M+PK VILSENNM C CS+CG+F TGF+RRVEYLWRFLDSTSSAFK R+SDER++MEG
Sbjct: 421 RGMEPKGVILSENNMECCCSSCGDFATGFSRRVEYLWRFLDSTSSAFKNRDSDERKMMEG 480

Query: 481 EAAKALMNQGEMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKD 540
           EAAKAL NQ EMNE +EKWCERMK AGFA + F ED ID  RA +R+YDNNWEM++EE  
Sbjct: 481 EAAKALTNQREMNERREKWCERMKEAGFAGEVFGEDAIDGGRALLRKYDNNWEMKVEENS 540

Query: 541 GCVGLWWKGQPVSFCSFWKL 548
             V LWWK QPVSFCS WKL
Sbjct: 541 TSVELWWKSQPVSFCSLWKL 549

BLAST of Lag0003137 vs. ExPASy Swiss-Prot
Match: A1DQP9 (Protein NODULATION SIGNALING PATHWAY 1 OS=Lotus japonicus OX=34305 GN=NSP1 PE=2 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 1.3e-179
Identity = 351/566 (62.01%), Postives = 417/566 (73.67%), Query Frame = 0

Query: 1   MTIEEPGPNH-PSDHILDWLEDSVPFFSPFLEETYNSSS-INCYQWWDENQDMGQDLING 60
           MT+E   PN   SDHILDWLE SV FF  FL+E  N+S  I  Y  WD+ Q    D   G
Sbjct: 1   MTME---PNSTTSDHILDWLEGSVSFFPSFLDEPCNNSGYIQEYHLWDQYQ---TDANTG 60

Query: 61  CL--SSSPTTVTTVSTRPPNTPT-----SHHLTP-SDLTKKRKAPDETVHKTSQSHQNQR 120
               +++ TT T V+T   +T +     S++  P SDL KKR A DE+  K  Q+   + 
Sbjct: 61  SSPNATNSTTATIVATSATSTTSLEPCGSNNNQPLSDLPKKRNATDESSLKPPQNKNKRI 120

Query: 121 KNQ-NNQSKNGADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNP 180
           K +  N+ +NG             ++K   NKK  +K+ G+N N+G++KEGRWAEQLLNP
Sbjct: 121 KTRPMNEPENG-----------DAVRK---NKKGGAKANGSNCNSGNSKEGRWAEQLLNP 180

Query: 181 CANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTV 240
           CA AI  G+  RV HLL VL ELASP GD NHRLAAHGLRAL HHLSS+SSS +SS +  
Sbjct: 181 CAAAIAGGNVNRVQHLLYVLHELASPTGDPNHRLAAHGLRALTHHLSSSSSSPTSSGT-- 240

Query: 241 APVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPS-RSRNLHILDIGV 300
             +TFAST+PRFFQ+SL+KF+EVSPWF+FPNNIAN+SIL +L+EE +  SR LHILDIGV
Sbjct: 241 --ITFASTEPRFFQKSLLKFYEVSPWFSFPNNIANASILQVLAEEANITSRTLHILDIGV 300

Query: 301 SHGVQWPTLLEALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFA 360
           SHGVQWPTLL+AL+RRSGGPP ++RLTV+  T E+DQN ETPFS  PPG N   RLL +A
Sbjct: 301 SHGVQWPTLLDALSRRSGGPPSVVRLTVV--TAENDQNMETPFSKAPPGYNYYPRLLGYA 360

Query: 361 KSLNINLQINRLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLR 420
           +S+NINLQINR++NHSLQ+LN+Q I+  PDEILIVCAQFRLH L H +PDER EFL+ LR
Sbjct: 361 QSININLQINRIENHSLQTLNAQSISASPDEILIVCAQFRLHHLNHNSPDERSEFLKVLR 420

Query: 421 KMDPKAVILSENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGE 480
            M+P+ VILSENN  C CS CGNF  GFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGE
Sbjct: 421 NMEPRGVILSENNTECCCSGCGNFAAGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGE 480

Query: 481 AAKALMNQGEMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDG 540
           AAKAL NQ EMNEEKEKWC RMK AGFA + F ED +D  RA +R+YD+NWEM++EEK+ 
Sbjct: 481 AAKALTNQREMNEEKEKWCGRMKEAGFAGEVFGEDAVDGGRALLRKYDSNWEMKVEEKNT 540

Query: 541 CVGLWWKGQPVSFCSFWKLGMKSNGG 555
            VGLWWKGQPVSFCS WKL     GG
Sbjct: 541 SVGLWWKGQPVSFCSLWKLDGNDQGG 540

BLAST of Lag0003137 vs. ExPASy Swiss-Prot
Match: Q9LRW3 (Scarecrow-like protein 29 OS=Arabidopsis thaliana OX=3702 GN=SCL29 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.1e-125
Identity = 269/555 (48.47%), Postives = 348/555 (62.70%), Query Frame = 0

Query: 4   EEPGPNHPSDHILDWLEDSVPFFS-PFLEETYNSSSINCYQ-W-WDENQD--------MG 63
           E   PN   DH+L WLEDSV     P  +++Y     +  Q W WD+ QD          
Sbjct: 5   ETEPPNQTLDHVLSWLEDSVSLSPLPGFDDSYLLHEFDGSQTWEWDQTQDPEHGFIQSYS 64

Query: 64  QDLINGCLSSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKN 123
           QDL    +    T +  V+  P            DL  + + P++   K  +SH    + 
Sbjct: 65  QDLSAAYVGCEATNLEVVTEAPS--------IDLDLPPEIQQPNDQSRK--RSHDGFLEA 124

Query: 124 QNNQSKNGADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCAN 183
           Q                    +KKS  +K+ + KS+  +  +G NKEGRWAE+LLNPCA 
Sbjct: 125 QQ-------------------VKKSARSKRKAIKSSEKSSKDG-NKEGRWAEKLLNPCAL 184

Query: 184 AIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPV 243
           AI   +++RV H LCVL ELAS  GDAN RLAA GLRAL HHLSS     SS SS+  PV
Sbjct: 185 AITASNSSRVQHYLCVLSELASSSGDANRRLAAFGLRALQHHLSS-----SSVSSSFWPV 244

Query: 244 -TFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHG 303
            TFAS + + FQ++L+KF+EVSPWFA PNN+ANS+IL IL+++P   ++LHI+DIGVSHG
Sbjct: 245 FTFASAEVKMFQKTLLKFYEVSPWFALPNNMANSAILQILAQDPKDKKDLHIIDIGVSHG 304

Query: 304 VQWPTLLEALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSL 363
           +QWPTLLEAL+ R  GPPP +R+TVI+     D   + PFS+GPPG N  S+LL FA+SL
Sbjct: 305 MQWPTLLEALSCRLEGPPPRVRITVIS-----DLTADIPFSVGPPGYNYGSQLLGFARSL 364

Query: 364 NINLQINRLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMD 423
            INLQI+ LD         Q+I   P E LIVCAQFRLH L+H   DER E L+ +R + 
Sbjct: 365 KINLQISVLD-------KLQLIDTSPHENLIVCAQFRLHHLKHSINDERGETLKAVRSLR 424

Query: 424 PKAVILSENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAK 483
           PK V+L ENN  CS S   +F  GF++++EY+W+FLDSTSS FK   S+ER++MEGEA K
Sbjct: 425 PKGVVLCENNGECSSS--ADFAAGFSKKLEYVWKFLDSTSSGFKEENSEERKLMEGEATK 484

Query: 484 ALMNQGEMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVG 543
            LMN G+MNE KEKW ERM+ AGF  + F ED +D A++ +R+YDNNWE+R+E+ D   G
Sbjct: 485 VLMNAGDMNEGKEKWYERMREAGFFVEAFEEDAVDGAKSLLRKYDNNWEIRMEDGDTFAG 510

Query: 544 LWWKGQPVSFCSFWK 547
           L WKG+ VSFCS WK
Sbjct: 545 LMWKGEAVSFCSLWK 510

BLAST of Lag0003137 vs. ExPASy Swiss-Prot
Match: Q84MQ9 (Protein NODULATION SIGNALING PATHWAY 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP1 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 5.2e-83
Identity = 206/523 (39.39%), Postives = 285/523 (54.49%), Query Frame = 0

Query: 44  WWDENQDMGQDLINGCLSS--SPTTVTTVSTRPPNTPTSHHLTPSDL----TKKRKAPDE 103
           WW  +    QD I   ++   SP +    +   P+  +    +PSD+    +KKRK+P  
Sbjct: 46  WWAASPAAQQDDIGSVVAQTLSPPSTAAPAAASPSIASPAASSPSDVPSSSSKKRKSP-- 105

Query: 104 TVHKTSQSHQNQRKNQNNQSKNGADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSN 163
                     ++        K G  KGGG                            GS+
Sbjct: 106 ---------AHRAPGHTGGKKGGGGKGGG---------------------------GGSD 165

Query: 164 KEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSS 223
           ++ RWAEQLLNPCA A+  G+ +RV HL  VL EL S  GDANHRLAAHGLRALA  L +
Sbjct: 166 RDMRWAEQLLNPCAVAVEAGNLSRVQHLFYVLGELESFSGDANHRLAAHGLRALARWLPA 225

Query: 224 NSSSTSSSSSTVAP------VTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILH-- 283
                ++++  V P        FA+ +PR F+ SLI+FHEVSPWFA PN +AN++I    
Sbjct: 226 AVGPAAAAAVRVPPCSERPTTAFAAAEPRLFRASLIRFHEVSPWFALPNALANAAIAQAS 285

Query: 284 ILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGG-PPPLIRLTVIAPTVEHDQNTE 343
                 +  R LH++D+GVSHGVQWPTLLE+LTR+ GG  PP +RLTV+ P      +  
Sbjct: 286 TCGAAGATPRPLHVVDLGVSHGVQWPTLLESLTRQPGGRAPPSVRLTVVGPGAT-ATSPV 345

Query: 344 TPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQSLNSQVIAKFPDEILIVCAQFR 403
            PFS  PPG + S  LL +AKS+N++L+I+R       +     +     E L+VC QFR
Sbjct: 346 APFSASPPGYDFSPHLLRYAKSINLDLRISR------AATLDDAVPGDDGEALVVCLQFR 405

Query: 404 LHQLRHYAPDERFEFLQNLRKMDPKAVILSE--NNMACSCSNCGNFDTGFTRRVEYLWRF 463
              L H A +ER E L+  R ++P+ V+LSE  + +     + G+    F  R+E LWRF
Sbjct: 406 ---LGHAAAEERREVLRKARGLNPELVVLSELDSGVGVVGGDGGSAAGEFAARLELLWRF 465

Query: 464 LDSTSSAFKGRESDERRVMEGEAAK--ALMNQGEMNEEKEKWCERMKNAGFARKFFAEDT 523
           L+STS+AFKG++ +ERR++E EA    A  +     E +E W ERM  AGF    F  + 
Sbjct: 466 LESTSAAFKGKDVEERRLLEAEAGAILAAADVAAAGEGREGWRERMAAAGFEEAPFGAEA 520

Query: 524 IDTARASMRRYDNNWEMRI-EEKDGCVGLWWKGQPVSFCSFWK 547
           +++AR+ +R+YD+ WEM         V L WKGQPVSFCS W+
Sbjct: 526 VESARSLLRKYDSGWEMSAPSPAAAAVALRWKGQPVSFCSLWR 520

BLAST of Lag0003137 vs. ExPASy Swiss-Prot
Match: Q9SN22 (Scarecrow-like protein 32 OS=Arabidopsis thaliana OX=3702 GN=SCL32 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.2e-36
Identity = 123/409 (30.07%), Postives = 193/409 (47.19%), Query Frame = 0

Query: 159 EGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSN 218
           +  + EQLL  CA AI   DA   H +L VL  +A P GD+  RL +  LRAL   LS  
Sbjct: 23  DANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRAL---LSRA 82

Query: 219 SSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRS 278
            S T + SST++ +  A    RF    L  F +++PW  F    AN++IL  +    +  
Sbjct: 83  VSKTPTLSSTISFLPQADELHRFSVVELAAFVDLTPWHRFGFIAANAAILTAVEGYST-- 142

Query: 279 RNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGD 338
             +HI+D+ ++H +Q PTL++A+  R   PPPL++LTV++       +   P  I    +
Sbjct: 143 --VHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVS------SSDHFPPFINISYE 202

Query: 339 NISSRLLSFAKSLNINLQINRLDNHSLQSLNS--QVIAKFP---DEILIVCAQFRLHQLR 398
            + S+L++FA + NI ++   + +      +S  Q +  +P   +E L+V      H + 
Sbjct: 203 ELGSKLVNFATTRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVV----NCHMML 262

Query: 399 HYAPDE---------RFEFLQNLRKMDPKAVILSENNMACSCSNCGNFDTGFTRRVEYLW 458
            Y P+E         R  FL+ LR ++P+ V L E ++  +  N  N          Y W
Sbjct: 263 RYIPEEPLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVN---RLKSAFNYFW 322

Query: 459 RFLDSTSSAFKGRESDERRVMEGE--------AAKALMNQGEMNEEKEKWCERMKNAGFA 518
              D+T +      S++RR  E E         AK    + E  E K +W ERM+ A F 
Sbjct: 323 IPFDTTDTFM----SEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFG 382

Query: 519 RKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFW 546
                ED +   +A +  +   W M+ E+ D  + L WKG  V F + W
Sbjct: 383 GVRVKEDAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHSVVFATVW 407

BLAST of Lag0003137 vs. ExPASy TrEMBL
Match: A0A6J1KER4 (nodulation-signaling pathway 1 protein OS=Cucurbita maxima OX=3661 GN=LOC111494340 PE=3 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.7e-291
Identity = 505/555 (90.99%), Postives = 528/555 (95.14%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFF-SPFLEETYNSSSINCYQWWDENQDMGQDLINGC 60
           MTIEEPG NHPSDHILDWL DSVPFF SPF +++YNSSSINCYQWWDENQD+GQDLINGC
Sbjct: 40  MTIEEPGANHPSDHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGC 99

Query: 61  LSSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKN 120
           LSSSP   TTVST+PPNT TSHHLTPSDLTKKRKAPD+TVHK SQ+ QNQRKNQNNQSKN
Sbjct: 100 LSSSP---TTVSTKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKN 159

Query: 121 GADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDA 180
           GADKG GAVEGVTVIKKSVGNK+NSSK+TGNN +NGSNKEGRWAEQLLNPCANAIIKGDA
Sbjct: 160 GADKGSGAVEGVTVIKKSVGNKRNSSKATGNNNHNGSNKEGRWAEQLLNPCANAIIKGDA 219

Query: 181 TRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDP 240
           TRVHHLLCVLQELASP GDANHRLAA+GLRALAH+LSSN SS  SSSSTVAPVTFASTD 
Sbjct: 220 TRVHHLLCVLQELASPTGDANHRLAAYGLRALAHYLSSN-SSCYSSSSTVAPVTFASTDL 279

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEP+RSRNLHILDIGVSHGVQWPTLLE
Sbjct: 280 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLE 339

Query: 301 ALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTVIAPTVEHDQN ETPFSIGPPGDNISSRLLSFAKSLNINLQINR
Sbjct: 340 ALTRRSGGPPPLIRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 399

Query: 361 LDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSE 420
           LDN SLQS NSQVI K PDEILIVCAQFRLHQL+HYAPDERFEFLQNLRK++PKAVILSE
Sbjct: 400 LDNRSLQSFNSQVIGKLPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSE 459

Query: 421 NNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEM 480
           NNMACSC+NCGNFDTGFTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAA+ L NQGEM
Sbjct: 460 NNMACSCNNCGNFDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAARGLTNQGEM 519

Query: 481 NEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEE EKWCERM+NAGFARK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPV
Sbjct: 520 NEEMEKWCERMRNAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPV 579

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLGMKSNGG
Sbjct: 580 SFCSFWKLGMKSNGG 590

BLAST of Lag0003137 vs. ExPASy TrEMBL
Match: A0A6J1HKE2 (nodulation-signaling pathway 1 protein OS=Cucurbita moschata OX=3662 GN=LOC111464355 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.5e-282
Identity = 489/543 (90.06%), Postives = 518/543 (95.40%), Query Frame = 0

Query: 13  DHILDWLEDSVPFF-SPFLEETYNSSSINCYQWWDENQDMGQDLINGCLSSSPTTVTTVS 72
           DHILDWL DSVPFF SPF +++YNSSSINCYQWWDENQD+GQDLINGCLSSSP   TTVS
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNGADKGGGAVEGV 132
           T+PPNT TSHHLTPSDLTKKRKAPD+TVHK SQ+ QNQRKNQNNQS+NGADK  GAV GV
Sbjct: 104 TKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSRNGADKSSGAVAGV 163

Query: 133 TVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 192
           TV+KKSVGNK+NSSK+TGNN NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGNNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHE 252
           LASP GDANHRLAA+GLRALAH+LSSNSS   SSSST+APVTFASTDPRFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSNSS--CSSSSTLAPVTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFAFPNNIANSSILHILSEEP+RSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQSLNSQ 372
           IRLTVIAPTVEHDQN ETPFSIGPPGDNISSRLLSFAKSLNINLQINRLD+ SL SLN+Q
Sbjct: 344 IRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDSLSLLSLNAQ 403

Query: 373 VIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSENNMACSCSNCGN 432
           VI KFPDEILIVCAQFRLHQL+HYAPDERFEFLQNLRK++PKAVILSENNMACSC+NCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSENNMACSCTNCGN 463

Query: 433 FDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEMNEEKEKWCERMK 492
           FDTGFTR+VEYLWRFLDSTSSAFKGRES+ER++MEGEAAKAL N+GEMNEE EKWCERM+
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKLMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKLGMKS 552
           NAGFARK F EDTIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQPVSFCSFWKLGMKS
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGMKS 581

Query: 553 NGG 555
           NGG
Sbjct: 584 NGG 581

BLAST of Lag0003137 vs. ExPASy TrEMBL
Match: A0A0A0KCK6 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450500 PE=3 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 8.9e-280
Identity = 489/555 (88.11%), Postives = 515/555 (92.79%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLEETYNSSSINCYQWWDENQDMGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDSVPFFS FL+ET NSSSINCYQWWDENQD G+DLINGCL
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 60

Query: 61  SSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNG 120
           S+SPTTV  VSTR PNTPTSH LTPSDLTKKRKAPD++VHK SQ+HQN RKNQNNQSKN 
Sbjct: 61  SNSPTTV--VSTRQPNTPTSHSLTPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNS 120

Query: 121 ADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG GAVEGVTV+KKSVGNKKN+SKSTGNNYN+GSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 121 ADKGSGAVEGVTVMKKSVGNKKNASKSTGNNYNSGSNKEGRWAEQLLNPCANAIVKGDAT 180

Query: 181 RVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSST--SSSSSTVAPVTFASTD 240
           RVHHLLCVLQELASP GDANHRLA HGLRALA+HLSSNSSS+  SS SSTVAP TFASTD
Sbjct: 181 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVAPFTFASTD 240

Query: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLL 300
           PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEE +R RNLHILDIGVSHGVQWPTLL
Sbjct: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHILDIGVSHGVQWPTLL 300

Query: 301 EALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360
           EALTRRSGGPPPLIRLTVIAPT+EHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN
Sbjct: 301 EALTRRSGGPPPLIRLTVIAPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360

Query: 361 RLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILS 420
           RLD HSLQSLNSQ I K  DEILIVCAQFRLHQL+H APDER EFL+NLRKM+PKAVILS
Sbjct: 361 RLDIHSLQSLNSQAINKSRDEILIVCAQFRLHQLKHSAPDERQEFLENLRKMEPKAVILS 420

Query: 421 ENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMN-QG 480
           ENNM CSCS CGNF+ GF R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N  G
Sbjct: 421 ENNMGCSCSKCGNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVDG 480

Query: 481 EMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQ 540
           EMNEEK KWCERM+N GF RK F EDTIDTARASMRRYDNNWEMR+E+KDGCVGLWWKGQ
Sbjct: 481 EMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKGQ 540

Query: 541 PVSFCSFWKLGMKSN 553
           PVSFCSFWKLG+KSN
Sbjct: 541 PVSFCSFWKLGIKSN 553

BLAST of Lag0003137 vs. ExPASy TrEMBL
Match: A0A1S3CGQ3 (nodulation-signaling pathway 1 protein OS=Cucumis melo OX=3656 GN=LOC103500695 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.3e-278
Identity = 487/555 (87.75%), Postives = 515/555 (92.79%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLEETYNSSSINCYQWWDENQDMGQDLINGCL 60
           MTIEE GP+HPSDHILDWLEDSVPFFS FL+ET NSSSINCYQWWDENQD G+DLINGCL
Sbjct: 44  MTIEETGPDHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 103

Query: 61  SSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNG 120
           S+SPTTV  VSTR PNTPTSHHL PSDLTKKRKAPD++VHK SQ+HQN RKNQNNQSKN 
Sbjct: 104 SNSPTTV--VSTRQPNTPTSHHLPPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNI 163

Query: 121 ADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG GAVEGVTVIKKSVGNKKN+SKSTGNNYNNGSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 164 ADKGSGAVEGVTVIKKSVGNKKNASKSTGNNYNNGSNKEGRWAEQLLNPCANAIVKGDAT 223

Query: 181 RVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSST--SSSSSTVAPVTFASTD 240
           RVHHLLCVLQELASP GDANHRLA HGLRALA+HLSSNSSS+  SS SSTV+P+TFASTD
Sbjct: 224 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVSPITFASTD 283

Query: 241 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLL 300
           PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEE +R RNLH+LDIGVSHGVQWPTLL
Sbjct: 284 PRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHVLDIGVSHGVQWPTLL 343

Query: 301 EALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 360
           EALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN
Sbjct: 344 EALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQIN 403

Query: 361 RLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILS 420
           RLD HSLQSLNSQ I K  DEILIVC+QFRLHQL+H APDER EFLQNLRKM+PKAVILS
Sbjct: 404 RLDIHSLQSLNSQAINKSRDEILIVCSQFRLHQLKHSAPDERQEFLQNLRKMEPKAVILS 463

Query: 421 ENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMN-QG 480
           ENNM CSCS C NF+ GF R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N +G
Sbjct: 464 ENNMGCSCSKCVNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVEG 523

Query: 481 EMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQ 540
           EMNEEK KWCERM+N GF RK F EDTIDTARASMRRYDNNWEMR+E+KDGCVGLWWKGQ
Sbjct: 524 EMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKGQ 583

Query: 541 PVSFCSFWKLGMKSN 553
           PVSFCS WKLG+KSN
Sbjct: 584 PVSFCSLWKLGIKSN 596

BLAST of Lag0003137 vs. ExPASy TrEMBL
Match: A0A6J1D4T6 (nodulation-signaling pathway 1 protein OS=Momordica charantia OX=3673 GN=LOC111017018 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.7e-278
Identity = 488/555 (87.93%), Postives = 510/555 (91.89%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSVPFFSPFLEETYNSSSINCYQWWDENQDMGQDLINGCL 60
           MTIEEPGPNHPSDHILDWLEDS PFFSPFL+ETYNSSSINCYQWWDE+Q++GQDLINGCL
Sbjct: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60

Query: 61  SSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKNQNNQSKNG 120
           SSSP   TT ST PPNT +   LTPSDL+KKRKAPD+T HKT+Q HQN RKNQNNQSKNG
Sbjct: 61  SSSPAAATTDSTTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 120

Query: 121 ADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKGGG      V+KKSVGNKK+SSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT
Sbjct: 121 ADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180

Query: 181 RVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAP-VTFASTDP 240
           RVHHL+CVLQELASP GDANHRLA HGLRALAHHLSSNS   SSSSST+AP V FASTD 
Sbjct: 181 RVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNS---SSSSSTLAPVVNFASTDA 240

Query: 241 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFA PNNIANSSILH LSEEP+ SRNLHILDIGVSHGVQWPTLLE
Sbjct: 241 RFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLE 300

Query: 301 ALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTV+APTVEHDQ  ETPFSIGPPGDNISSRLLSFAKSLNINLQINR
Sbjct: 301 ALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360

Query: 361 LDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSE 420
           LDNHSLQ+LNSQVI KF DEILIVCA FRLHQL+H APDER EFL+NLRKM+P AVILSE
Sbjct: 361 LDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSE 420

Query: 421 NNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALMNQGEM 480
           NN+ACSCSNCGNFD  FTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKAL NQGEM
Sbjct: 421 NNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480

Query: 481 NEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPV 540
           NEEKEKW ERM+NAGFARKFFAE TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+
Sbjct: 481 NEEKEKWYERMRNAGFARKFFAEXTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPI 540

Query: 541 SFCSFWKLGMKSNGG 555
           SFCSFWKLG K NGG
Sbjct: 541 SFCSFWKLGAKPNGG 544

BLAST of Lag0003137 vs. TAIR 10
Match: AT3G13840.1 (GRAS family transcription factor )

HSP 1 Score: 451.8 bits (1161), Expect = 7.8e-127
Identity = 269/555 (48.47%), Postives = 348/555 (62.70%), Query Frame = 0

Query: 4   EEPGPNHPSDHILDWLEDSVPFFS-PFLEETYNSSSINCYQ-W-WDENQD--------MG 63
           E   PN   DH+L WLEDSV     P  +++Y     +  Q W WD+ QD          
Sbjct: 5   ETEPPNQTLDHVLSWLEDSVSLSPLPGFDDSYLLHEFDGSQTWEWDQTQDPEHGFIQSYS 64

Query: 64  QDLINGCLSSSPTTVTTVSTRPPNTPTSHHLTPSDLTKKRKAPDETVHKTSQSHQNQRKN 123
           QDL    +    T +  V+  P            DL  + + P++   K  +SH    + 
Sbjct: 65  QDLSAAYVGCEATNLEVVTEAPS--------IDLDLPPEIQQPNDQSRK--RSHDGFLEA 124

Query: 124 QNNQSKNGADKGGGAVEGVTVIKKSVGNKKNSSKSTGNNYNNGSNKEGRWAEQLLNPCAN 183
           Q                    +KKS  +K+ + KS+  +  +G NKEGRWAE+LLNPCA 
Sbjct: 125 QQ-------------------VKKSARSKRKAIKSSEKSSKDG-NKEGRWAEKLLNPCAL 184

Query: 184 AIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTVAPV 243
           AI   +++RV H LCVL ELAS  GDAN RLAA GLRAL HHLSS     SS SS+  PV
Sbjct: 185 AITASNSSRVQHYLCVLSELASSSGDANRRLAAFGLRALQHHLSS-----SSVSSSFWPV 244

Query: 244 -TFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVSHG 303
            TFAS + + FQ++L+KF+EVSPWFA PNN+ANS+IL IL+++P   ++LHI+DIGVSHG
Sbjct: 245 FTFASAEVKMFQKTLLKFYEVSPWFALPNNMANSAILQILAQDPKDKKDLHIIDIGVSHG 304

Query: 304 VQWPTLLEALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSL 363
           +QWPTLLEAL+ R  GPPP +R+TVI+     D   + PFS+GPPG N  S+LL FA+SL
Sbjct: 305 MQWPTLLEALSCRLEGPPPRVRITVIS-----DLTADIPFSVGPPGYNYGSQLLGFARSL 364

Query: 364 NINLQINRLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLRKMD 423
            INLQI+ LD         Q+I   P E LIVCAQFRLH L+H   DER E L+ +R + 
Sbjct: 365 KINLQISVLD-------KLQLIDTSPHENLIVCAQFRLHHLKHSINDERGETLKAVRSLR 424

Query: 424 PKAVILSENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAK 483
           PK V+L ENN  CS S   +F  GF++++EY+W+FLDSTSS FK   S+ER++MEGEA K
Sbjct: 425 PKGVVLCENNGECSSS--ADFAAGFSKKLEYVWKFLDSTSSGFKEENSEERKLMEGEATK 484

Query: 484 ALMNQGEMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVG 543
            LMN G+MNE KEKW ERM+ AGF  + F ED +D A++ +R+YDNNWE+R+E+ D   G
Sbjct: 485 VLMNAGDMNEGKEKWYERMREAGFFVEAFEEDAVDGAKSLLRKYDNNWEIRMEDGDTFAG 510

Query: 544 LWWKGQPVSFCSFWK 547
           L WKG+ VSFCS WK
Sbjct: 545 LMWKGEAVSFCSLWK 510

BLAST of Lag0003137 vs. TAIR 10
Match: AT3G49950.1 (GRAS family transcription factor )

HSP 1 Score: 153.7 bits (387), Expect = 4.4e-37
Identity = 123/409 (30.07%), Postives = 193/409 (47.19%), Query Frame = 0

Query: 159 EGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSN 218
           +  + EQLL  CA AI   DA   H +L VL  +A P GD+  RL +  LRAL   LS  
Sbjct: 23  DANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRAL---LSRA 82

Query: 219 SSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRS 278
            S T + SST++ +  A    RF    L  F +++PW  F    AN++IL  +    +  
Sbjct: 83  VSKTPTLSSTISFLPQADELHRFSVVELAAFVDLTPWHRFGFIAANAAILTAVEGYST-- 142

Query: 279 RNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGD 338
             +HI+D+ ++H +Q PTL++A+  R   PPPL++LTV++       +   P  I    +
Sbjct: 143 --VHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVS------SSDHFPPFINISYE 202

Query: 339 NISSRLLSFAKSLNINLQINRLDNHSLQSLNS--QVIAKFP---DEILIVCAQFRLHQLR 398
            + S+L++FA + NI ++   + +      +S  Q +  +P   +E L+V      H + 
Sbjct: 203 ELGSKLVNFATTRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVV----NCHMML 262

Query: 399 HYAPDE---------RFEFLQNLRKMDPKAVILSENNMACSCSNCGNFDTGFTRRVEYLW 458
            Y P+E         R  FL+ LR ++P+ V L E ++  +  N  N          Y W
Sbjct: 263 RYIPEEPLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVN---RLKSAFNYFW 322

Query: 459 RFLDSTSSAFKGRESDERRVMEGE--------AAKALMNQGEMNEEKEKWCERMKNAGFA 518
              D+T +      S++RR  E E         AK    + E  E K +W ERM+ A F 
Sbjct: 323 IPFDTTDTFM----SEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFG 382

Query: 519 RKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFW 546
                ED +   +A +  +   W M+ E+ D  + L WKG  V F + W
Sbjct: 383 GVRVKEDAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHSVVFATVW 407

BLAST of Lag0003137 vs. TAIR 10
Match: AT4G37650.1 (GRAS family transcription factor )

HSP 1 Score: 125.9 bits (315), Expect = 9.8e-29
Identity = 140/540 (25.93%), Postives = 235/540 (43.52%), Query Frame = 0

Query: 47  ENQDMGQDLINGCLSSSPTTVTTVSTRPPNTPTSHHLTPSDLTKK--RKAPDETVHKTSQ 106
           + Q     +I    S S T+ TT  T  P T   ++   +D+ ++      DE    +S 
Sbjct: 11  QQQQQSDSIITNQSSLSRTSTTT--TGSPQTAYHYNFPQNDVVEECFNFFMDEEDLSSSS 70

Query: 107 SHQNQRKNQN--------------NQSKNGADKGGGAVEGVTVIKKSVGNKKNSS----K 166
           SH N   + N              + + +       A   +     S G+  + S     
Sbjct: 71  SHHNHHNHNNPNTYYSPFTTPTQYHPATSSTPSSTAAAAALASPYSSSGHHNDPSAFSIP 130

Query: 167 STGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAH 226
            T  +++  +N   +WA+ +L   A A    D  R   +L  L EL+SP GD   +LA++
Sbjct: 131 QTPPSFDFSAN--AKWADSVLLEAARAFSDKDTARAQQILWTLNELSSPYGDTEQKLASY 190

Query: 227 GLRALAHHLSSNSS----STSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNNI 286
            L+AL + ++ +      +  ++++T    +F ST     +++++KF EVSPW  F +  
Sbjct: 191 FLQALFNRMTGSGERCYRTMVTAAATEKTCSFEST-----RKTVLKFQEVSPWATFGHVA 250

Query: 287 ANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLT--VIAPT 346
           AN +IL  +  E      +HI+DI  +   QWPTLLEAL  RS   P L RLT  V+A  
Sbjct: 251 ANGAILEAVDGE----AKIHIVDISSTFCTQWPTLLEALATRSDDTPHL-RLTTVVVANK 310

Query: 347 VEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDN-HSLQSLNSQVIAKFPDE 406
             +DQ              I +R+  FA+ + +  + N + +   L   +   +   PDE
Sbjct: 311 FVNDQTASHRMM-----KEIGNRMEKFARLMGVPFKFNIIHHVGDLSEFDLNELDVKPDE 370

Query: 407 ILIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSENNMACSCSNCGNFDTGFTRR 466
           +L +     +H +       R   + + R++ P+ V + E          G FD  F R 
Sbjct: 371 VLAINCVGAMHGIASRG-SPRDAVISSFRRLRPRIVTVVEEEADLVGEEEGGFDDEFLRG 430

Query: 467 VEYLWRF----LDSTSSAFKGRESDERRVMEGEAAKALM--------NQGEMNEEKEKWC 526
                R+     +S   +F  R S+ER ++E  A +A++        +  E  E   KW 
Sbjct: 431 FGECLRWFRVCFESWEESFP-RTSNERLMLERAAGRAIVDLVACEPSDSTERRETARKWS 490

Query: 527 ERMKNAGFARKFFAEDTIDTARASMRRY-DNNWEMRIEEKDGCVGLWWKGQPVSFCSFWK 547
            RM+N+GF    ++++  D  RA +RRY +  W M        + L W+ QPV + S W+
Sbjct: 491 RRMRNSGFGAVGYSDEVADDVRALLRRYKEGVWSMVQCPDAAGIFLCWRDQPVVWASAWR 529

BLAST of Lag0003137 vs. TAIR 10
Match: AT5G66770.1 (GRAS family transcription factor )

HSP 1 Score: 120.6 bits (301), Expect = 4.1e-27
Identity = 112/392 (28.57%), Postives = 177/392 (45.15%), Query Frame = 0

Query: 170 CANAIIKGDATRVHHLLCVLQELASPIGDANHRLAAHGLRALAHHLSSNSSSTSSSSSTV 229
           CA  I   D       L  ++E  S +GD   R+A +   AL++ LS NS +TSSSSS+ 
Sbjct: 224 CAR-ISDSDPNEASKTLLQIRESVSELGDPTERVAFYFTEALSNRLSPNSPATSSSSSST 283

Query: 230 APVTFASTDPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPSRSRNLHILDIGVS 289
             +            S    ++  P+  F +  AN +IL    E   +S  +HI+D G+ 
Sbjct: 284 EDLIL----------SYKTLNDACPYSKFAHLTANQAIL----EATEKSNKIHIVDFGIV 343

Query: 290 HGVQWPTLLEALTRRSGGPPPLIRLTVI-APTVEHDQNTETPFSIGPPGDNISSRLLSFA 349
            G+QWP LL+AL  R+ G P  IR++ I AP++      E+P    P      +RL  FA
Sbjct: 344 QGIQWPALLQALATRTSGKPTQIRVSGIPAPSL-----GESP---EPSLIATGNRLRDFA 403

Query: 350 KSLNINLQINRLDNHSLQSLNSQVIAKFPDEILIVCAQFRLHQLRHYAPDERFEFLQNLR 409
           K L++N     +    +  LN       PDE+L V    +L++L    P      L+  +
Sbjct: 404 KVLDLNFDFIPILT-PIHLLNGSSFRVDPDEVLAVNFMLQLYKLLDETPTIVDTALRLAK 463

Query: 410 KMDPKAVILSENNMACSCSNCGNFDTGFTRRVEYLWRFLDSTSSAFK---GRESDER--- 469
            ++P+ V L E  ++ +         GF  RV+   +F  +   + +   GR+S+ER   
Sbjct: 464 SLNPRVVTLGEYEVSLN-------RVGFANRVKNALQFYSAVFESLEPNLGRDSEERVRV 523

Query: 470 -RVMEGEAAKALMN------QGEMNEEKEKWCERMKNAGFARKFFAEDTIDTARASMRRY 529
            R + G     L+         E  EEKE+W   M+NAGF     +   +  A+  +  Y
Sbjct: 524 ERELFGRRISGLIGPEKTGIHRERMEEKEQWRVLMENAGFESVKLSNYAVSQAKILLWNY 583

Query: 530 D-NNWEMRIEEKDGCVGLWWKGQPVSFCSFWK 547
           + +N    +E K G + L W   P+   S W+
Sbjct: 584 NYSNLYSIVESKPGFISLAWNDLPLLTLSSWR 584

BLAST of Lag0003137 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 106.3 bits (264), Expect = 8.0e-23
Identity = 106/415 (25.54%), Postives = 183/415 (44.10%), Query Frame = 0

Query: 142 KNSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQELASPIGDANH 201
           ++S +ST +     S + G      L  CA AI + +      L+  +  LA     A  
Sbjct: 159 ESSDESTRSVVLVDSQETGVRLVHALVACAEAIHQENLNLADALVKRVGTLAGSQAGAMG 218

Query: 202 RLAAHGLRALAHHLSSNSSSTSSSSSTVAPVTFASTDPRFFQRSLIKFHEVSPWFAFPNN 261
           ++A +  +ALA  +  + ++ +           A+ +P F +   + F+E  P+  F + 
Sbjct: 219 KVATYFAQALARRIYRDYTAETD--------VCAAVNPSFEEVLEMHFYESCPYLKFAHF 278

Query: 262 IANSSILHILSEEPSRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVIAPTV 321
            AN +IL    E  + +R +H++D+G++ G+QWP L++AL  R GGPP   RLT I P  
Sbjct: 279 TANQAIL----EAVTTARRVHVIDLGLNQGMQWPALMQALALRPGGPPS-FRLTGIGP-- 338

Query: 322 EHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQSLNSQVIAKFPD-EI 381
                TE   S+      +  +L  FA+++ +  +   L   SL  L  ++    P+ E 
Sbjct: 339 ---PQTENSDSL----QQLGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESET 398

Query: 382 LIVCAQFRLHQLRHYAPDERFEFLQNLRKMDPKAVILSENNMACSCSNCGNFDTGFTRRV 441
           L+V + F LH+L   +     + L  ++ + P  V + E     +  N   F   F   +
Sbjct: 399 LVVNSVFELHRLLARSGSIE-KLLNTVKAIKPSIVTVVEQE---ANHNGIVFLDRFNEAL 458

Query: 442 EYLWRFLDSTSSAFKGRESDE--------RRVMEGEAAKALMNQGEMNEEKEKWCERMKN 501
            Y     DS   ++     D         R+++   AA+   ++ E +E   +W  RMK+
Sbjct: 459 HYYSSLFDSLEDSYSLPSQDRVMSEVYLGRQILNVVAAEG-SDRVERHETAAQWRIRMKS 518

Query: 502 AGFARKFFAEDTIDTARASMRRYDNNWEMRIEEKDGCVGLWWKGQPVSFCSFWKL 548
           AGF            A   +  Y      R+EE DGC+ + W+ +P+   S WKL
Sbjct: 519 AGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGCLMIGWQTRPLITTSAWKL 546