Lag0003079 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003079 vs. NCBI nr
Match: XP_022154722.1 (uncharacterized protein LOC111021905 [Momordica charantia])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.6e-296
Identity = 504/563 (89.52%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQ--FHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNG 60
           MASIPSHG RHLST RCSFQ  FHF KLRS+TS+AASLSPT+PQGDSERAEQ  PS+PNG
Sbjct: 1   MASIPSHGFRHLSTARCSFQFNFHFHKLRSTTSVAASLSPTLPQGDSERAEQNGPSNPNG 60

Query: 61  LSSKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKG 120
           LS KSQSYFPKRGQT+ELVCESLAFKGKGLCKVADTGFVVMCDRALPGERF GRVTR KG
Sbjct: 61  LSRKSQSYFPKRGQTLELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFFGRVTRMKG 120

Query: 121 NYAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFS 180
           NYAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKE QVHELLTHVGKF 
Sbjct: 121 NYAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKEQQVHELLTHVGKFY 180

Query: 181 QKELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGF 240
           QKELES NV+K IVPC+IQFHYRNKMEFSFGSKRWLP+DALIEK+ GD+INALGLHAPGF
Sbjct: 181 QKELESHNVLKHIVPCDIQFHYRNKMEFSFGSKRWLPRDALIEKQYGDEINALGLHAPGF 240

Query: 241 FDKVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKT 300
           FDKVLNV+KCLLQS PANQVL S+QECWR+PQLGLSPYDVHSHKGFLKHLMLRTGRDVKT
Sbjct: 241 FDKVLNVNKCLLQSGPANQVLESIQECWRDPQLGLSPYDVHSHKGFLKHLMLRTGRDVKT 300

Query: 301 GLPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST 360
           GLPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST
Sbjct: 301 GLPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST 360

Query: 361 IREILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTL 420
           I EILRGLTFQISANSFFQTNT QAEVLY LIEECA LKGDGSEIVLDLFCGTGTIGLTL
Sbjct: 361 ITEILRGLTFQISANSFFQTNTHQAEVLYNLIEECAGLKGDGSEIVLDLFCGTGTIGLTL 420

Query: 421 AKRAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDP 480
           AKRAKHVYGYEVV QAI+DA                 DLNKIDE+FGNNFPKPDIIISDP
Sbjct: 421 AKRAKHVYGYEVVPQAIKDACLNAKLNIICNATFVQGDLNKIDESFGNNFPKPDIIISDP 480

Query: 481 NRPGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVD 540
           NRPGMH+KLIKFLLK+KAPRIVYVSCNPATCARDLDYLCHGVV+QGIKGCYKLKSLQPVD
Sbjct: 481 NRPGMHMKLIKFLLKIKAPRIVYVSCNPATCARDLDYLCHGVVDQGIKGCYKLKSLQPVD 540

Query: 541 MFPHTPHIECVCLLELTPDSQMG 546
           MFPHTPHIECVCLLEL PDSQ+G
Sbjct: 541 MFPHTPHIECVCLLELIPDSQLG 563

BLAST of Lag0003079 vs. NCBI nr
Match: XP_004141670.1 (uncharacterized protein LOC101215920 [Cucumis sativus] >KGN45547.1 hypothetical protein Csa_016446 [Cucumis sativus])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 497/562 (88.43%), Postives = 515/562 (91.64%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASIPSHGLRHLSTTRCSFQFHF K RSSTSLAAS S T    DS+RAEQ DP S NG S
Sbjct: 1   MASIPSHGLRHLSTTRCSFQFHFHKFRSSTSLAASPSSTTSHDDSQRAEQHDPPSSNGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
           SKSQSYFPKRGQT+ELVCESLAFKGKGLCKV+DTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQ+R KE QVHELLTHVGKFSQK
Sbjct: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           ELE   VMK IVPCEIQFHYRNKMEFSFGSKRW+PKDAL EK++GD+INALGLHAPGFFD
Sbjct: 181 ELEFHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEKQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLNVDKCLLQS PANQVLASVQECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDV TGL
Sbjct: 241 KVLNVDKCLLQSGPANQVLASVQECWRDPLLGLSPYDVHSHKGFLKHLMLRTGRDVNTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEK SDIPEVVS+VNN+NSSVGNTSVGEEEHVLYGKS+IR
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKFSDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ESLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVV QAI+DA                 DLNKID+NFGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVPQAIKDACLNAELNSICNATFVQGDLNKIDQNFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+K PRIVYVSCNPATCARDLDYLCHGVVE+GIKGCY LKSLQPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKTPRIVYVSCNPATCARDLDYLCHGVVEEGIKGCYTLKSLQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. NCBI nr
Match: KAA0059451.1 (putative RNA methyltransferase BT_0643 isoform X1 [Cucumis melo var. makuwa] >TYK03873.1 putative RNA methyltransferase BT_0643 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.7e-291
Identity = 495/562 (88.08%), Postives = 516/562 (91.81%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASIPSHGLRH+ST RCSFQFHF K RSSTSLAASLS T    DS+RAEQ DP S +G S
Sbjct: 1   MASIPSHGLRHVSTARCSFQFHFHKFRSSTSLAASLSSTTSHDDSQRAEQHDPPSSSGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
           SKSQSYFPKRGQT+ELVCESLAFKGKGLCKV+DTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AEV KLETLSP WDLVDAPCEYASYCGGCKTQNLSYEAQ+R KE QVHELLTHVGKFSQK
Sbjct: 121 AEVAKLETLSPPWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           ELES  VMK IVPCEIQFHYRNKMEFSFGSKRW+PKDAL E+++GD+INALGLHAPGFFD
Sbjct: 181 ELESHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEEQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLNVDKCLLQS PANQVLASVQECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDVKTGL
Sbjct: 241 KVLNVDKCLLQSGPANQVLASVQECWRDPPLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEKISDIPEVVS+VNN+NSSVGNTSVGEEEHVLYGKS+IR
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ENLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVV QAI+DA                 DLNKID+NFGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVPQAIKDACLNAELNSICNATFVQGDLNKIDQNFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+K PRIVYVSCNPATCARDLDYLCHGVVE+GIKGCY LKSLQPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKTPRIVYVSCNPATCARDLDYLCHGVVEEGIKGCYTLKSLQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. NCBI nr
Match: XP_008462378.1 (PREDICTED: uncharacterized RNA methyltransferase BT_0643 isoform X1 [Cucumis melo])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.0e-291
Identity = 494/562 (87.90%), Postives = 516/562 (91.81%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASIPSHGLRH+ST RCSFQFHF K RSSTSLAASLS T    DS+RAEQ DP S +G S
Sbjct: 1   MASIPSHGLRHVSTARCSFQFHFHKFRSSTSLAASLSSTTSHDDSQRAEQHDPPSSSGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
           SKSQSYFPKRGQT+ELVCESLAFKGKGLCKV+DTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AEV KLETLSP WDLVDAPCEYASYCGGCKTQNLSYEAQ+R KE QVHELLTHVGKFSQK
Sbjct: 121 AEVAKLETLSPPWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           +LES  VMK IVPCEIQFHYRNKMEFSFGSKRW+PKDAL E+++GD+INALGLHAPGFFD
Sbjct: 181 DLESHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEEQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLNVDKCLLQS PANQVLASVQECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDVKTGL
Sbjct: 241 KVLNVDKCLLQSGPANQVLASVQECWRDPPLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEKISDIPEVVS+VNN+NSSVGNTSVGEEEHVLYGKS+IR
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ENLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVV QAI+DA                 DLNKID+NFGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVPQAIKDACLNAELNSICNATFVQGDLNKIDQNFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+K PRIVYVSCNPATCARDLDYLCHGVVE+GIKGCY LKSLQPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKTPRIVYVSCNPATCARDLDYLCHGVVEEGIKGCYTLKSLQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. NCBI nr
Match: XP_022992221.1 (uncharacterized protein LOC111488608 [Cucurbita maxima])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.3e-288
Identity = 489/562 (87.01%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASI SHG RHLSTTRCSFQFHF KLR+STSLAAS S TI   DSERAE  +P + NG S
Sbjct: 1   MASISSHGFRHLSTTRCSFQFHFHKLRTSTSLAASPSSTISHDDSERAELHEPPTSNGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
            +SQ YFPKRGQT+ELVCESLAFKGKGLCK ADTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  KQSQPYFPKRGQTLELVCESLAFKGKGLCKAADTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AE TKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKE QVHELLTHVGKFS K
Sbjct: 121 AEATKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKEQQVHELLTHVGKFSMK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           ELES +V+K+IVPCEIQFHYRNKMEFSFGSKRWLPK+  IEK++GD+INALGLHAPGFFD
Sbjct: 181 ELESHSVLKAIVPCEIQFHYRNKMEFSFGSKRWLPKEVFIEKQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLN+DKCLLQS PANQVLAS+QECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDVKTGL
Sbjct: 241 KVLNIDKCLLQSGPANQVLASIQECWRDPLLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEKISDIPEVVS+VNN+NSSVGNTSVGE EHVLYGKSTI 
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSVVNNINSSVGNTSVGEVEHVLYGKSTIT 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ENLRGLTFQISANSFFQTNTCQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVVAQAI+DA                 DLNKIDE+FGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVAQAIKDACLNAKLNSICNATFVQGDLNKIDESFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+KAPRIVYVSCNPATCARDLDYLCHGV E+GIKGCY LKS+QPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKAPRIVYVSCNPATCARDLDYLCHGVEEKGIKGCYTLKSVQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. ExPASy Swiss-Prot
Match: Q8KGF9 (Uncharacterized RNA methyltransferase CT0009 OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=CT0009 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.2e-77
Identity = 185/490 (37.76%), Postives = 266/490 (54.29%), Query Frame = 0

Query: 69  KRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNYAEVTKLET 128
           ++G  +EL     A K K   K  +   V++     PG+R   ++ + K  Y E   +E 
Sbjct: 13  RKGDIIELTITDHAEKDKCFGKTTEGMGVMVSGILAPGDRVSAQIYKVKSRYLEARAIEV 72

Query: 129 LSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQKELESQNVM 188
           L    D V+  C     CGGCK  ++SYEAQ+R K  +V + L H+G F   +      +
Sbjct: 73  LEASPDRVEPVCPVFGSCGGCKWMHVSYEAQLRYKHKKVTDSLEHIGGFESPD------V 132

Query: 189 KSIVPCEIQFHYRNKMEFSFGSKRWLPKDAL-IEKEDGDKINALGLHAPGFFDKVLNVDK 248
           + ++      HYRNK+EFS  + R+L +  +  ++    K  ALG HAPG F+KVL++D 
Sbjct: 133 RPVLAAPDALHYRNKVEFSCSNMRYLLQSEIDSDQLAKPKTFALGFHAPGNFEKVLDLDT 192

Query: 249 CLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGLPELMVNF 308
           C L  E  N+VL  +++     + GL PY   +H+G+L++LMLR     +    +LMVN 
Sbjct: 193 CYLAKECMNRVLNVLRDF--AIERGLEPYAAKAHEGYLRNLMLRYSERHE----QLMVNI 252

Query: 309 VTSSYKPELLMPLVEKI-SDIPE-VVSIVNNVNSSVGNTSVGEEEHVLYGKSTIREILRG 368
           VTS Y   L+  L E++ + +PE  ++++NNV +     + GE+E+V+ G   + E L  
Sbjct: 253 VTSWYDKALMQALKERLEAAMPEQQMTLLNNVTTRKNTVATGEQEYVISGDGYVTERLGD 312

Query: 369 LTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAKRAKHV 428
           L F+ISANSFFQTNTRQAE LY  I     +  +  + V DL+CGTGTI L LA+  K  
Sbjct: 313 LDFRISANSFFQTNTRQAETLYDQIIAVGGITPE--DTVYDLYCGTGTITLYLARHCKQA 372

Query: 429 YGYEVVAQAIEDA---WDLNKID---------ENFG------NNFPKPDIIISDPNRPGM 488
            G EVV  A++DA    +LN +          +NF         + KP II++DP R GM
Sbjct: 373 IGIEVVESAVKDAEMNAELNGLSNTVFFQADLKNFHAMQEALEPYAKPRIIVTDPPRAGM 432

Query: 489 HVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMFPHT 538
           H K +  +LK++  RIVYVSCNP   ARD         E   +G Y++ S QPVDMFP T
Sbjct: 433 HPKALDTMLKLQPERIVYVSCNPDNLARD-------GKEIAARG-YRMTSAQPVDMFPQT 480

BLAST of Lag0003079 vs. ExPASy Swiss-Prot
Match: Q8AA22 (Uncharacterized RNA methyltransferase BT_0643 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=BT_0643 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.6e-76
Identity = 179/480 (37.29%), Postives = 274/480 (57.08%), Query Frame = 0

Query: 81  LAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY--AEVTKLETLSPHWDLVDA 140
           +A +GK + KV D   V+     +PG+    ++ R+K  Y  AE  K   LSP   +   
Sbjct: 1   MAAEGKAIAKVND--LVIFVPYVVPGDVVDLQIKRKKNKYAEAEAVKFHELSP---VRAV 60

Query: 141 P-CEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQKELESQNVMKSIVPCEIQ 200
           P C++   CGGCK Q L Y  Q+R K+ QV + L  +GK     +E   +   +   + +
Sbjct: 61  PFCQHYGVCGGCKWQVLPYSEQIRYKQKQVEDNLRRIGK-----IELPEISPILGSAKTE 120

Query: 201 FHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFDKVLNVDKCLLQSEPANQ 260
           F YRNK+EF+F +KRWL  D + +    D++NA+G H PG FDKVL ++KC LQ + +N+
Sbjct: 121 F-YRNKLEFTFSNKRWLTNDEVRQDVKYDQMNAVGFHIPGAFDKVLAIEKCWLQDDISNR 180

Query: 261 VLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTG-LPELMVNFVTSSYKPEL 320
           +  +V++     +   S  ++ + +G L++L++RT     TG L  +++  +T  ++ EL
Sbjct: 181 IRNAVRD--YAYEHDYSFINLRTQEGMLRNLIIRTS---STGELMVIVICKITEDHEMEL 240

Query: 321 LMPLVEKISD-IPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIREILRGLTFQISANSF 380
              L++ I+D  PE+ S++  +N+   +T    + HV  GK  + E + GL F++   SF
Sbjct: 241 FKQLLQFIADSFPEITSLLYIINNKCNDTINDLDVHVFKGKDHMFEEMEGLRFKVGPKSF 300

Query: 381 FQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAKRAKHVYGYEVVAQAI 440
           +QTN+ QA  LY++  E A L   G E+V DL+ GTGTI   ++++A+ V G E V +AI
Sbjct: 301 YQTNSEQAYNLYKIAREFAGL--TGKELVYDLYTGTGTIANFVSRQARQVIGIEYVPEAI 360

Query: 441 EDA---WDLNKI--------------DENFGNNFPKPDIIISDPNRPGMHVKLIKFLLKV 500
           EDA    ++N+I               + F N   +PD+II+DP R GMH  ++  +L  
Sbjct: 361 EDAKVNAEINEIKNALFYAGDMKDMLTQEFINQHGRPDVIITDPPRAGMHQDVVDVILFA 420

Query: 501 KAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMFPHTPHIECVCLLEL 539
           +  RIVYVSCNPAT ARDL         Q + G YK+K++QPVDMFPHT H+E V LLEL
Sbjct: 421 EPKRIVYVSCNPATQARDL---------QLLDGKYKVKAVQPVDMFPHTHHVENVVLLEL 453

BLAST of Lag0003079 vs. ExPASy Swiss-Prot
Match: Q9KF10 (Uncharacterized RNA methyltransferase BH0687 OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=BH0687 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.4e-72
Identity = 177/490 (36.12%), Postives = 258/490 (52.65%), Query Frame = 0

Query: 70  RGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNYAEVTKLETL 129
           +   VE+  E L   G G+ KV   G+ +   +ALPGER   +V + K  Y     L  +
Sbjct: 10  KNDVVEVTIEDLTHDGAGVAKV--DGYALFIPKALPGERLKAKVVKVKKGYGFGRVLNMI 69

Query: 130 SPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQKELESQNVMK 189
               D V+APC   + CGGC+ Q++SY+AQ+R K+ QV ++L  +GK +         ++
Sbjct: 70  EASPDRVEAPCPVFNQCGGCQLQHMSYDAQLRYKQKQVQDVLERIGKITAV------TVR 129

Query: 190 SIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD----KVLNV 249
             +     + YRNK +   G           E+E G           GF+     +++++
Sbjct: 130 PTIGMNEPWRYRNKAQVPVG-----------EREGG--------LIAGFYQERSHRIIDM 189

Query: 250 DKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGLPELMV 309
           D+C++Q E  ++V+  V+E  R  +LG+  YD   H+G L+H++ R G++  TG  E+MV
Sbjct: 190 DECMIQHEENDKVIRQVKELAR--ELGIRGYDEEKHRGTLRHVVARYGKN--TG--EIMV 249

Query: 310 NFVTSSYKPELLMPLVEKI-SDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIREILR 369
             +T   +      L+E+I   IP V SIV NVN    N   G++  VL+G+  I + + 
Sbjct: 250 VLITRGEELPHKKTLIERIHKAIPHVKSIVQNVNPKRTNVIFGDKTKVLWGEEYIYDTIG 309

Query: 370 GLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAKRAKH 429
            + F ISA SF+Q N  Q +VLY    E A+L   GSE V+D +CG GTI L LA++AKH
Sbjct: 310 DIKFAISARSFYQVNPEQTKVLYDQALEFANL--TGSETVIDAYCGIGTISLFLAQQAKH 369

Query: 430 VYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNRPGMH 489
           VYG E+V +AI DA                 D  K+   +     + D+I+ DP R G  
Sbjct: 370 VYGVEIVPEAISDAKRNARLNGFANVQFAVGDAEKVMPWWYAQGVRADVIVVDPPRKGCD 429

Query: 490 VKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMFPHTP 539
             L+K +L +K  R+VYVSCNPAT ARDL  L          G Y+ K +QPVDMFP T 
Sbjct: 430 EALLKTILNMKPDRVVYVSCNPATLARDLRVL--------EDGGYETKDVQPVDMFPWTT 456

BLAST of Lag0003079 vs. ExPASy Swiss-Prot
Match: Q7MVG9 (Uncharacterized RNA methyltransferase PG_1095 OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=PG_1095 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.3e-72
Identity = 182/486 (37.45%), Postives = 273/486 (56.17%), Query Frame = 0

Query: 81  LAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNYAE--VTKLETLSPHWDLVDA 140
           LA +G  LC++ D   V+    A PG+R   +V ++K N+ +  +  +E+ SP   +   
Sbjct: 6   LAAEGNALCRIDD--MVMFVPFAAPGDRCTVQVVKKKRNFMQGRIVSIESPSP---IRKG 65

Query: 141 P-CEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQKELESQNVMKSIVPCEIQ 200
           P C + + CGGCK Q++ YE Q+R+KE QV + L  +GK   +E      M+ I+  E  
Sbjct: 66  PFCTHFTVCGGCKWQHIPYEIQLRAKEQQVTDALVRLGKVEVEE------MQPILGSEQT 125

Query: 201 FHYRNKMEFSFGSKRWLPKDALIEKEDGD----KINALGLHAPGFFDKVLNVDKCLLQSE 260
            +YRNK+EF+F +KRWL  +  +++ DGD        LG H PG FDKVL++ +C L ++
Sbjct: 126 EYYRNKLEFTFSNKRWLLPEE-VQEVDGDFSPEVRYGLGFHIPGMFDKVLDIRECHLGAK 185

Query: 261 PANQVLASVQE-CWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGLPELMVNFV---- 320
            ++++   ++E C ++P+     +D+ + +G ++ LM+RT     T   ELMV  V    
Sbjct: 186 VSDEIRLFIREYCMQDPE-RYPFFDLRNQEGLMRTLMIRT-----TSTGELMVVVVFFRD 245

Query: 321 TSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLY-GKSTIREILRGLT 380
             + +  LL  + E+    P++ S++  +N+   N ++G++E +LY G+  I E + GL 
Sbjct: 246 DVAAREALLAAVAER---FPQITSLLYVINTKC-NDTIGDQEVLLYHGREYIEEEMEGLK 305

Query: 381 FQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAKRAKHVYG 440
           F+I A SF+QTN+RQA  LYR+  E A L GD  E+V DL+ GTGTI   +A +A  V G
Sbjct: 306 FKIGAKSFYQTNSRQAYQLYRIAREFAGLTGD--ELVYDLYTGTGTIANFVAGQAHRVIG 365

Query: 441 YEVVAQAIEDAWD---LNKID--------------ENFGNNFPKPDIIISDPNRPGMHVK 500
            E V +AIEDA     LN I+               +F     +PD++I+DP R GMH  
Sbjct: 366 IEYVPEAIEDARTNSLLNGIENTLFYAGDMKDILTNDFIEQHGRPDVVITDPPRAGMHES 425

Query: 501 LIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMFPHTPHI 537
           +I  +L     RIVYVSCNPAT ARDL  L          G Y+    +PVDMFPHT H+
Sbjct: 426 VIDAILFAAPRRIVYVSCNPATQARDLALLM-------ADGRYRAVKSRPVDMFPHTHHV 460

BLAST of Lag0003079 vs. ExPASy Swiss-Prot
Match: Q71YR7 (Uncharacterized RNA methyltransferase LMOf2365_1776 OS=Listeria monocytogenes serotype 4b (strain F2365) OX=265669 GN=LMOf2365_1776 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.0e-68
Identity = 169/496 (34.07%), Postives = 252/496 (50.81%), Query Frame = 0

Query: 69  KRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY--AEVTKL 128
           K+ Q++EL  E L   G G+ K+   G+ +     LPGE+   ++ +   NY  A +  +
Sbjct: 7   KKNQSIELTIEDLTHDGSGVGKI--DGYPLFIPNTLPGEKVTAKIIKLNKNYGFARMENI 66

Query: 129 ETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQKELESQN 188
           ET+S   D V+ PC   S CGGC  Q+LSY+ Q+  K  QV E +  +GK + +  E+  
Sbjct: 67  ETVSA--DRVEPPCAVYSKCGGCSLQHLSYDGQLEFKRNQVEETMKRIGKLNVEVPETLG 126

Query: 189 VMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFDK----V 248
           +       E  + YRNK +   G                  +N  G    GF+ K    +
Sbjct: 127 M-------ENPWRYRNKSQVPVGF-----------------VN--GKLTAGFYQKRSHAI 186

Query: 249 LNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGLPE 308
           +++  CL+ +E  +  +   +E     + G  PYD  + KG ++H+M R      TG  +
Sbjct: 187 IDMSTCLIHNEQGDFAVQKTREIL--AKYGTEPYDEQTGKGDIRHIMTRFAH--TTG--Q 246

Query: 309 LMVNFVTSS----YKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST 368
           LM+  VT+     +K E++  LVE++    E+ SIV N+N    N   G+    L+GK  
Sbjct: 247 LMIVLVTTKERMPFKEEIVRELVEQL----ELTSIVQNINPHKTNVIFGDRTKTLWGKDI 306

Query: 369 IREILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTL 428
           I + + G+ F ISA SF+Q N  Q EVLY+   + A+L G+  E V+D +CG G+I L L
Sbjct: 307 IEDTIHGIRFAISARSFYQVNPIQTEVLYQQAIDAAELTGE--ETVIDAYCGIGSISLCL 366

Query: 429 AKRAKHVYGYEVVAQAIEDAWDLNKIDENFGNNFPK----------------PDIIISDP 488
           AK+AKHVYG E+V QAI+DA    +++E     F                   D+++ DP
Sbjct: 367 AKKAKHVYGVEIVDQAIQDARANAELNELANTTFETGKAEEVIPAWYKAGIVADVLVVDP 426

Query: 489 NRPGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVD 539
            R G   KL++ +L +K  ++VYVSCNP T ARD+  L          G Y  K +QPVD
Sbjct: 427 PRKGCDEKLLETILAMKPKKVVYVSCNPGTLARDMKIL--------TDGGYVAKKVQPVD 452

BLAST of Lag0003079 vs. ExPASy TrEMBL
Match: A0A6J1DME4 (uncharacterized protein LOC111021905 OS=Momordica charantia OX=3673 GN=LOC111021905 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.8e-296
Identity = 504/563 (89.52%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQ--FHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNG 60
           MASIPSHG RHLST RCSFQ  FHF KLRS+TS+AASLSPT+PQGDSERAEQ  PS+PNG
Sbjct: 1   MASIPSHGFRHLSTARCSFQFNFHFHKLRSTTSVAASLSPTLPQGDSERAEQNGPSNPNG 60

Query: 61  LSSKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKG 120
           LS KSQSYFPKRGQT+ELVCESLAFKGKGLCKVADTGFVVMCDRALPGERF GRVTR KG
Sbjct: 61  LSRKSQSYFPKRGQTLELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFFGRVTRMKG 120

Query: 121 NYAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFS 180
           NYAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKE QVHELLTHVGKF 
Sbjct: 121 NYAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKEQQVHELLTHVGKFY 180

Query: 181 QKELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGF 240
           QKELES NV+K IVPC+IQFHYRNKMEFSFGSKRWLP+DALIEK+ GD+INALGLHAPGF
Sbjct: 181 QKELESHNVLKHIVPCDIQFHYRNKMEFSFGSKRWLPRDALIEKQYGDEINALGLHAPGF 240

Query: 241 FDKVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKT 300
           FDKVLNV+KCLLQS PANQVL S+QECWR+PQLGLSPYDVHSHKGFLKHLMLRTGRDVKT
Sbjct: 241 FDKVLNVNKCLLQSGPANQVLESIQECWRDPQLGLSPYDVHSHKGFLKHLMLRTGRDVKT 300

Query: 301 GLPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST 360
           GLPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST
Sbjct: 301 GLPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKST 360

Query: 361 IREILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTL 420
           I EILRGLTFQISANSFFQTNT QAEVLY LIEECA LKGDGSEIVLDLFCGTGTIGLTL
Sbjct: 361 ITEILRGLTFQISANSFFQTNTHQAEVLYNLIEECAGLKGDGSEIVLDLFCGTGTIGLTL 420

Query: 421 AKRAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDP 480
           AKRAKHVYGYEVV QAI+DA                 DLNKIDE+FGNNFPKPDIIISDP
Sbjct: 421 AKRAKHVYGYEVVPQAIKDACLNAKLNIICNATFVQGDLNKIDESFGNNFPKPDIIISDP 480

Query: 481 NRPGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVD 540
           NRPGMH+KLIKFLLK+KAPRIVYVSCNPATCARDLDYLCHGVV+QGIKGCYKLKSLQPVD
Sbjct: 481 NRPGMHMKLIKFLLKIKAPRIVYVSCNPATCARDLDYLCHGVVDQGIKGCYKLKSLQPVD 540

Query: 541 MFPHTPHIECVCLLELTPDSQMG 546
           MFPHTPHIECVCLLEL PDSQ+G
Sbjct: 541 MFPHTPHIECVCLLELIPDSQLG 563

BLAST of Lag0003079 vs. ExPASy TrEMBL
Match: A0A0A0KB04 (TRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G452020 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.0e-293
Identity = 497/562 (88.43%), Postives = 515/562 (91.64%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASIPSHGLRHLSTTRCSFQFHF K RSSTSLAAS S T    DS+RAEQ DP S NG S
Sbjct: 1   MASIPSHGLRHLSTTRCSFQFHFHKFRSSTSLAASPSSTTSHDDSQRAEQHDPPSSNGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
           SKSQSYFPKRGQT+ELVCESLAFKGKGLCKV+DTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQ+R KE QVHELLTHVGKFSQK
Sbjct: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           ELE   VMK IVPCEIQFHYRNKMEFSFGSKRW+PKDAL EK++GD+INALGLHAPGFFD
Sbjct: 181 ELEFHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEKQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLNVDKCLLQS PANQVLASVQECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDV TGL
Sbjct: 241 KVLNVDKCLLQSGPANQVLASVQECWRDPLLGLSPYDVHSHKGFLKHLMLRTGRDVNTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEK SDIPEVVS+VNN+NSSVGNTSVGEEEHVLYGKS+IR
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKFSDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ESLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVV QAI+DA                 DLNKID+NFGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVPQAIKDACLNAELNSICNATFVQGDLNKIDQNFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+K PRIVYVSCNPATCARDLDYLCHGVVE+GIKGCY LKSLQPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKTPRIVYVSCNPATCARDLDYLCHGVVEEGIKGCYTLKSLQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. ExPASy TrEMBL
Match: A0A5D3BVW7 (Putative RNA methyltransferase BT_0643 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00680 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 495/562 (88.08%), Postives = 516/562 (91.81%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASIPSHGLRH+ST RCSFQFHF K RSSTSLAASLS T    DS+RAEQ DP S +G S
Sbjct: 1   MASIPSHGLRHVSTARCSFQFHFHKFRSSTSLAASLSSTTSHDDSQRAEQHDPPSSSGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
           SKSQSYFPKRGQT+ELVCESLAFKGKGLCKV+DTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AEV KLETLSP WDLVDAPCEYASYCGGCKTQNLSYEAQ+R KE QVHELLTHVGKFSQK
Sbjct: 121 AEVAKLETLSPPWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           ELES  VMK IVPCEIQFHYRNKMEFSFGSKRW+PKDAL E+++GD+INALGLHAPGFFD
Sbjct: 181 ELESHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEEQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLNVDKCLLQS PANQVLASVQECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDVKTGL
Sbjct: 241 KVLNVDKCLLQSGPANQVLASVQECWRDPPLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEKISDIPEVVS+VNN+NSSVGNTSVGEEEHVLYGKS+IR
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ENLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVV QAI+DA                 DLNKID+NFGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVPQAIKDACLNAELNSICNATFVQGDLNKIDQNFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+K PRIVYVSCNPATCARDLDYLCHGVVE+GIKGCY LKSLQPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKTPRIVYVSCNPATCARDLDYLCHGVVEEGIKGCYTLKSLQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. ExPASy TrEMBL
Match: A0A1S3CGW1 (uncharacterized RNA methyltransferase BT_0643 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500749 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 2.9e-291
Identity = 494/562 (87.90%), Postives = 516/562 (91.81%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASIPSHGLRH+ST RCSFQFHF K RSSTSLAASLS T    DS+RAEQ DP S +G S
Sbjct: 1   MASIPSHGLRHVSTARCSFQFHFHKFRSSTSLAASLSSTTSHDDSQRAEQHDPPSSSGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
           SKSQSYFPKRGQT+ELVCESLAFKGKGLCKV+DTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AEV KLETLSP WDLVDAPCEYASYCGGCKTQNLSYEAQ+R KE QVHELLTHVGKFSQK
Sbjct: 121 AEVAKLETLSPPWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           +LES  VMK IVPCEIQFHYRNKMEFSFGSKRW+PKDAL E+++GD+INALGLHAPGFFD
Sbjct: 181 DLESHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEEQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLNVDKCLLQS PANQVLASVQECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDVKTGL
Sbjct: 241 KVLNVDKCLLQSGPANQVLASVQECWRDPPLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEKISDIPEVVS+VNN+NSSVGNTSVGEEEHVLYGKS+IR
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ENLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVV QAI+DA                 DLNKID+NFGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVPQAIKDACLNAELNSICNATFVQGDLNKIDQNFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+K PRIVYVSCNPATCARDLDYLCHGVVE+GIKGCY LKSLQPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKTPRIVYVSCNPATCARDLDYLCHGVVEEGIKGCYTLKSLQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. ExPASy TrEMBL
Match: A0A6J1JSZ1 (uncharacterized protein LOC111488608 OS=Cucurbita maxima OX=3661 GN=LOC111488608 PE=3 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 6.1e-289
Identity = 489/562 (87.01%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQLDPSSPNGLS 60
           MASI SHG RHLSTTRCSFQFHF KLR+STSLAAS S TI   DSERAE  +P + NG S
Sbjct: 1   MASISSHGFRHLSTTRCSFQFHFHKLRTSTSLAASPSSTISHDDSERAELHEPPTSNGFS 60

Query: 61  SKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGNY 120
            +SQ YFPKRGQT+ELVCESLAFKGKGLCK ADTGFVVMCDR LPGERFVGRVTRRKGNY
Sbjct: 61  KQSQPYFPKRGQTLELVCESLAFKGKGLCKAADTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 121 AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQK 180
           AE TKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKE QVHELLTHVGKFS K
Sbjct: 121 AEATKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKEQQVHELLTHVGKFSMK 180

Query: 181 ELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFFD 240
           ELES +V+K+IVPCEIQFHYRNKMEFSFGSKRWLPK+  IEK++GD+INALGLHAPGFFD
Sbjct: 181 ELESHSVLKAIVPCEIQFHYRNKMEFSFGSKRWLPKEVFIEKQEGDEINALGLHAPGFFD 240

Query: 241 KVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300
           KVLN+DKCLLQS PANQVLAS+QECWR+P LGLSPYDVHSHKGFLKHLMLRTGRDVKTGL
Sbjct: 241 KVLNIDKCLLQSGPANQVLASIQECWRDPLLGLSPYDVHSHKGFLKHLMLRTGRDVKTGL 300

Query: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTIR 360
           PELMVNFVTSSYKPELLMPLVEKISDIPEVVS+VNN+NSSVGNTSVGE EHVLYGKSTI 
Sbjct: 301 PELMVNFVTSSYKPELLMPLVEKISDIPEVVSVVNNINSSVGNTSVGEVEHVLYGKSTIT 360

Query: 361 EILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLAK 420
           E LRGLTFQISANSFFQTNT QAEVLYRLIEECA LKGDGSEIVLDLFCGTGTIGLTLAK
Sbjct: 361 ENLRGLTFQISANSFFQTNTCQAEVLYRLIEECAGLKGDGSEIVLDLFCGTGTIGLTLAK 420

Query: 421 RAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPNR 480
           RA+HVYGYEVVAQAI+DA                 DLNKIDE+FGNNFPKPDIIISDPNR
Sbjct: 421 RARHVYGYEVVAQAIKDACLNAKLNSICNATFVQGDLNKIDESFGNNFPKPDIIISDPNR 480

Query: 481 PGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDMF 540
           PGMH+KLIKFLLK+KAPRIVYVSCNPATCARDLDYLCHGV E+GIKGCY LKS+QPVDMF
Sbjct: 481 PGMHMKLIKFLLKIKAPRIVYVSCNPATCARDLDYLCHGVEEKGIKGCYTLKSVQPVDMF 540

Query: 541 PHTPHIECVCLLELTPDSQMGS 547
           PHTPHIECVCLLELTPDSQMGS
Sbjct: 541 PHTPHIECVCLLELTPDSQMGS 562

BLAST of Lag0003079 vs. TAIR 10
Match: AT3G21300.1 (RNA methyltransferase family protein )

HSP 1 Score: 817.8 bits (2111), Expect = 5.3e-237
Identity = 402/555 (72.43%), Postives = 463/555 (83.42%), Query Frame = 0

Query: 1   MASIPSHGLRHLSTTRCSFQFHFRKLRSSTSLAASLSPTIPQGDSERAEQL-DPSSPNGL 60
           +A+ P+HGLR++     S+   FR   SS    A LSP+   G ++  ++L D ++ N  
Sbjct: 2   LAATPAHGLRYVVVPTRSWLHRFRCCSSS---LAPLSPSEFSGGNKAQKKLNDENNTNVS 61

Query: 61  SSKSQSYFPKRGQTVELVCESLAFKGKGLCKVADTGFVVMCDRALPGERFVGRVTRRKGN 120
           + K+  Y+PKRG+TVELVCESL FKGKG+CKV  TG+VVMCDRALPGERF+GRVTRRKG+
Sbjct: 62  NEKTAPYYPKRGETVELVCESLGFKGKGICKVDGTGYVVMCDRALPGERFLGRVTRRKGS 121

Query: 121 YAEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQVRSKELQVHELLTHVGKFSQ 180
           YAEVTK++T+SPH DLV+APCEYASYCGGCKTQNLSYEAQ+R+KE QVHEL+ HVG+FS 
Sbjct: 122 YAEVTKMKTISPHKDLVEAPCEYASYCGGCKTQNLSYEAQLRAKEEQVHELIRHVGRFSD 181

Query: 181 KELESQNVMKSIVPCEIQFHYRNKMEFSFGSKRWLPKDALIEKEDGDKINALGLHAPGFF 240
                + V+K IV C+IQFHYRNKMEFSFG +RWLP + L E++DG K  ALGLHAPGFF
Sbjct: 182 NNPGLEIVLKPIVACDIQFHYRNKMEFSFGPQRWLPIEMLNERQDGPKNFALGLHAPGFF 241

Query: 241 DKVLNVDKCLLQSEPANQVLASVQECWRNPQLGLSPYDVHSHKGFLKHLMLRTGRDVKTG 300
           DKVLNVDKCLLQSEP N VLA+VQ+CWR+P+L LSPYD  SH GFLKHLMLRTGR+V+TG
Sbjct: 242 DKVLNVDKCLLQSEPGNLVLAAVQDCWRDPELSLSPYDCRSHVGFLKHLMLRTGRNVETG 301

Query: 301 LPELMVNFVTSSYKPELLMPLVEKISDIPEVVSIVNNVNSSVGNTSVGEEEHVLYGKSTI 360
             ELMVNFVTSSYKPELL PLV++IS IP+VVSI+NNVNSSVGNTSVGE+E+ LYGK TI
Sbjct: 302 SLELMVNFVTSSYKPELLKPLVDRISSIPQVVSIMNNVNSSVGNTSVGEKEYTLYGKDTI 361

Query: 361 REILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIGLTLA 420
            E+LRGLTFQISANSFFQTNT QAEVLY+LIEE A LKGDGSE+VLDLFCGTGTIGLTLA
Sbjct: 362 TEVLRGLTFQISANSFFQTNTHQAEVLYKLIEESAGLKGDGSEVVLDLFCGTGTIGLTLA 421

Query: 421 KRAKHVYGYEVVAQAIEDA----------------WDLNKIDENFGNNFPKPDIIISDPN 480
           +RAKHVYGYEVV QAI DA                 DLNKI E+FGNNFPKPDI+ISDPN
Sbjct: 422 RRAKHVYGYEVVPQAITDAHKNAQINGIENATFIQGDLNKIGEDFGNNFPKPDIVISDPN 481

Query: 481 RPGMHVKLIKFLLKVKAPRIVYVSCNPATCARDLDYLCHGVVEQGIKGCYKLKSLQPVDM 539
           RPGMH+KLIKFLLK+K+PRI+YVSCNPATCARDLDYLCHGV E+ +KGCYKL S+QPVDM
Sbjct: 482 RPGMHMKLIKFLLKLKSPRIIYVSCNPATCARDLDYLCHGVEEKNLKGCYKLMSVQPVDM 541

BLAST of Lag0003079 vs. TAIR 10
Match: AT2G28450.1 (zinc finger (CCCH-type) family protein )

HSP 1 Score: 79.7 bits (195), Expect = 7.9e-15
Identity = 80/296 (27.03%), Postives = 107/296 (36.15%), Query Frame = 0

Query: 356 KSTIREILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIG 415
           ++ I + +  L F IS  +FFQ NT  AE LY +  + ADL  D   ++ D+ CGTGTIG
Sbjct: 515 EARIHDHINNLRFSISPTAFFQVNTVTAEKLYSIAGDWADLGPD--TLLFDVCCGTGTIG 574

Query: 416 LTLAKRAKHVYGYEVVAQAIEDAW------------------------------------ 475
           LTLA R   V G E+ A A+ DA                                     
Sbjct: 575 LTLAHRVGMVIGIEMNASAVADAERNATINGISNCKFICSKAEDVMSSLLKQYLDVTQME 634

Query: 476 ----------DLNK--------------IDENF----------------------GNNFP 535
                     DLNK               D+N                            
Sbjct: 635 EAKPLSNANDDLNKQIPSTEEMTNSEHVADQNLPPSNTQVEELQDNEQKDSSSLEPEKTT 694

Query: 536 KPD----IIISDPNRPGMHVKLIKFL-LKVKAPRIVYVSCNPATCARDLDYLCHGVVEQG 538
           KP     + I DP R G+H  +IK L    +  R+VY+SCNP T   +   LC    ++ 
Sbjct: 695 KPQFKNVVAIVDPPRSGLHPAVIKALRTHPRLKRLVYISCNPETLVANAIELCTPSFDEP 754

BLAST of Lag0003079 vs. TAIR 10
Match: AT2G28450.2 (zinc finger (CCCH-type) family protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-11
Identity = 38/83 (45.78%), Postives = 50/83 (60.24%), Query Frame = 0

Query: 356 KSTIREILRGLTFQISANSFFQTNTRQAEVLYRLIEECADLKGDGSEIVLDLFCGTGTIG 415
           ++ I + +  L F IS  +FFQ NT  AE LY +  + ADL  D   ++ D+ CGTGTIG
Sbjct: 515 EARIHDHINNLRFSISPTAFFQVNTVTAEKLYSIAGDWADLGPD--TLLFDVCCGTGTIG 574

Query: 416 LTLAKRAKHVYGYEVVAQAIEDA 439
           LTLA R   V G E+ A A+ DA
Sbjct: 575 LTLAHRVGMVIGIEMNASAVADA 595