Lag0002821 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002821
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Non-specific lipid-transfer protein
Location	chr4: 45980353 .. 45981641 (+)
RNA-Seq Expression	Lag0002821
Synteny	Lag0002821

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTTTCATTTTGCTTGGAAATGGCTTTGGTTAAGCCTCCTGCTAACGTCACCGGCGGTGAGCTTTGTAGCTGGAGATGCAGAGAAAGACAAGGAGGAATGCACGCAGCAATTGGCTGGGATGGCCACGTGTCTGCCGTACGTGAGCGGTGACGCAAAGACTCCGACGCCCGACTGTTGCACTGGGCTGAAGGAAGTGCTTAAGAACAACAAGAAGTGCTTGTGCGTTATAGTTAAAGACCGAAATGACCCTGATTTGGGGCTTCAGATCAACGTCACACTTGCCTTGGGCCTGCCTGATGTTTGCCATGCCCCTGCTAACGTGTCCAATTGTCCAGGTAAACCGAACCGAACCGAACCAAATATTGAAATTTAACCAAATAATTTGATCTGATTAATTTTTTAGGTTAAATATATATTTTAGTACCTAATGTTTGAATATGTTTTTAATTAAATCTCTATTGTTTAAATGTTTCAATTTAGTCTATTATGTATAAACACTTTACAATTCAATCTCACACTATTGGTGAATGCTTAAATTTCCCAATAATAAGCTTACCTGACATATATTTAGTGAATTGAGAAAAAATTATAAGAGAAAAAACTCAAAATTTTTGTCAAATTAACTTTCATATTTTAGATGGCTTTTTTTCCTTCTCTAGAGTAGAATAAAAAACTTTCATCATTCATTGTTGAATGTTAAAATTGACTAATGATAAATTTATCTGATATAATTTTGAGTGAATTTGATAGAATTGTAGTAGAAGAACTAGAGCACTTAAAAAATAAAAAGAAAAATTATTCCTCCTAAGAATTTGTCAAAATTCATAGGATAAACTATTACCAACTTGAAAAACATATATTTAGCCATTTTTTTTTTTTAAAATATTCTAATGTGTTTTGTGAAAAAAAATAATAAAATTGGGTTTGGTGCAGCTCTTTTGAACTTGCCACCCAATTCACCAGAGGCTCAAGTTTTCTATCAATTTGGCAAGGCCAATAGCTCCTCTTCTGCTGTGGCAGCTGGTCCCATAAGCCCTTCTCCTAGTTCCACTGGTAATTGTGCCTCTCAACTTTCTAAATTTTTTTTTGGAGTTTAGGAAAGAAAATTATGGTCATTAATGGTTATTATTATTATTATTATTCAAAATTTCAGTTGGAGAGAAAAGCAGAACTGGACTAGATAAGGGTGAAGCTTTCAACTTAGGAAAGAAATGGCTGCCATTGGAGACTTTGGTTGTTGGATCTTTTTTGCTTTGCTTTTTGTTGGATTCATATCATGTGTAG

mRNA sequence

ATGGACTTTCATTTTGCTTGGAAATGGCTTTGGTTAAGCCTCCTGCTAACGTCACCGGCGGTGAGCTTTGTAGCTGGAGATGCAGAGAAAGACAAGGAGGAATGCACGCAGCAATTGGCTGGGATGGCCACGTGTCTGCCGTACGTGAGCGGTGACGCAAAGACTCCGACGCCCGACTGTTGCACTGGGCTGAAGGAAGTGCTTAAGAACAACAAGAAGTGCTTGTGCGTTATAGTTAAAGACCGAAATGACCCTGATTTGGGGCTTCAGATCAACGTCACACTTGCCTTGGGCCTGCCTGATGTTTGCCATGCCCCTGCTAACGTGTCCAATTGTCCAGCTCTTTTGAACTTGCCACCCAATTCACCAGAGGCTCAAGTTTTCTATCAATTTGGCAAGGCCAATAGCTCCTCTTCTGCTGTGGCAGCTGGTCCCATAAGCCCTTCTCCTAGTTCCACTGTTGGAGAGAAAAGCAGAACTGGACTAGATAAGGGTGAAGCTTTCAACTTAGGAAAGAAATGGCTGCCATTGGAGACTTTGGTTGTTGGATCTTTTTTGCTTTGCTTTTTGTTGGATTCATATCATGTGTAG

Coding sequence (CDS)

ATGGACTTTCATTTTGCTTGGAAATGGCTTTGGTTAAGCCTCCTGCTAACGTCACCGGCGGTGAGCTTTGTAGCTGGAGATGCAGAGAAAGACAAGGAGGAATGCACGCAGCAATTGGCTGGGATGGCCACGTGTCTGCCGTACGTGAGCGGTGACGCAAAGACTCCGACGCCCGACTGTTGCACTGGGCTGAAGGAAGTGCTTAAGAACAACAAGAAGTGCTTGTGCGTTATAGTTAAAGACCGAAATGACCCTGATTTGGGGCTTCAGATCAACGTCACACTTGCCTTGGGCCTGCCTGATGTTTGCCATGCCCCTGCTAACGTGTCCAATTGTCCAGCTCTTTTGAACTTGCCACCCAATTCACCAGAGGCTCAAGTTTTCTATCAATTTGGCAAGGCCAATAGCTCCTCTTCTGCTGTGGCAGCTGGTCCCATAAGCCCTTCTCCTAGTTCCACTGTTGGAGAGAAAAGCAGAACTGGACTAGATAAGGGTGAAGCTTTCAACTTAGGAAAGAAATGGCTGCCATTGGAGACTTTGGTTGTTGGATCTTTTTTGCTTTGCTTTTTGTTGGATTCATATCATGTGTAG

Protein sequence

MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPNSPEAQVFYQFGKANSSSSAVAAGPISPSPSSTVGEKSRTGLDKGEAFNLGKKWLPLETLVVGSFLLCFLLDSYHV

Homology

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BLAST of Lag0002821 vs. NCBI nr
Match: XP_038899115.1 (non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida])

HSP 1 Score: 333.6 bits (854), Expect = 1.2e-87
Identity = 164/196 (83.67%), Postives = 172/196 (87.76%), Query Frame = 0


Query: 3   FHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCT 62
           FHF WKWL L+LLLTSP VSFV GD EKDKEECTQQLAGMATCLPYVSG AK PTPDCC+
Sbjct: 4   FHFTWKWLLLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGGAKAPTPDCCS 63

Query: 63  GLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPNS 122
           GLKEVLKNNKKCLCVI+KDRNDPDLGLQINVTLALGLPD+CHA ANVSNCPALLNLPPNS
Sbjct: 64  GLKEVLKNNKKCLCVIIKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLPPNS 123

Query: 123 PEAQVFYQFGKANSSSSAVAAGPISPSP--SSTVGEKSRTGLDKGEAFNLGKKWLPLETL 182
            EAQVFYQ GK NSSSSAVA  PISPSP  +STVG  SR G DKG A+N+GKKWLPLETL
Sbjct: 124 AEAQVFYQLGKGNSSSSAVAPAPISPSPTINSTVGGGSRIGPDKGGAYNIGKKWLPLETL 183

Query: 183 VVGSFLLCFLLDSYHV 197
            VG+ LL F LDSYHV
Sbjct: 184 AVGALLLLFWLDSYHV 199

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BLAST of Lag0002821 vs. NCBI nr
Match: KAG6593339.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.5 bits (820), Expect = 1.0e-83
Identity = 159/197 (80.71%), Postives = 174/197 (88.32%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           M FHF  KWL L+LLL SPAVSF+AGDAEKD+EECTQQLAGMATCL YVSGDAK PTPDC
Sbjct: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C+GLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAVAAGPISPSPS-STVGEKSRTGLDKGEAFNLGKKWLPLET 180
           +SPEAQVFYQFGKA SSS+  AA PISPSP+ ++ G  SR G DKGEA+ +G KWLPL+T
Sbjct: 121 SSPEAQVFYQFGKAQSSSA--AAAPISPSPAINSTGGGSRNGTDKGEAYTIGNKWLPLKT 180

Query: 181 LVVGSFLLCFLLDSYHV 197
           LVVG+ L+  LLDSYHV
Sbjct: 181 LVVGALLVLSLLDSYHV 195

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BLAST of Lag0002821 vs. NCBI nr
Match: XP_022959693.1 (protein YLS3-like [Cucurbita moschata])

HSP 1 Score: 316.6 bits (810), Expect = 1.5e-82
Identity = 159/195 (81.54%), Postives = 172/195 (88.21%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           M FHFA KWL L+LLL SPAVSF+AGDAEKD+EECTQQLAGMATCL YVSGDAK PTPDC
Sbjct: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C+GLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAVAAGPISPSP--SSTVGEKSRTGLDKGEAFNLGKKWLPLE 180
           +SPEAQVFYQFGKA SSS+  AA PISPSP  SST G  SR G DKGEA+ +G KWLPL+
Sbjct: 121 SSPEAQVFYQFGKAQSSSA--AAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLK 180

Query: 181 TLVVGSFLLCFLLDS 194
           +LVV + L+ FLLDS
Sbjct: 181 SLVVAALLVLFLLDS 193

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BLAST of Lag0002821 vs. NCBI nr
Match: XP_023513729.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.8 bits (808), Expect = 2.5e-82
Identity = 158/195 (81.03%), Postives = 171/195 (87.69%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           M FHF  KWL L+LLL SPAVSF+AGDAEKD+EECTQQLAGMATCL YVSGDAK PTPDC
Sbjct: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C+GLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAVAAGPISPSP--SSTVGEKSRTGLDKGEAFNLGKKWLPLE 180
           NSPEAQVFYQFGKA SSS+  AA PISPSP  +ST G  SR G DKGEA+ +G KWLPL+
Sbjct: 121 NSPEAQVFYQFGKAQSSSA--AAAPISPSPAINSTGGGGSRNGTDKGEAYTIGNKWLPLK 180

Query: 181 TLVVGSFLLCFLLDS 194
           +LVV + L+ FLLDS
Sbjct: 181 SLVVAALLVLFLLDS 193

[Close]

BLAST of Lag0002821 vs. NCBI nr
Match: XP_022150545.1 (protein YLS3-like [Momordica charantia])

HSP 1 Score: 313.5 bits (802), Expect = 1.3e-81
Identity = 161/199 (80.90%), Postives = 170/199 (85.43%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           MDFHFA+K L L+LLLTSPAV  V  DAEKDKEECTQQLAGMATCLPYVSGDAK+PTPDC
Sbjct: 1   MDFHFAFKLLLLTLLLTSPAVRLVTADAEKDKEECTQQLAGMATCLPYVSGDAKSPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C GLKEVLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPD CHA ANVSNCPALLNLPP
Sbjct: 61  CNGLKEVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDSCHASANVSNCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAV-AAGPISPS--PSSTVGEKSRTGLDKGEAFNLGKKWLPL 180
           NSPEAQVFYQ GK+NSSS+A  AA PISPS   +STVG  SR G DKGEA   G KWLPL
Sbjct: 121 NSPEAQVFYQLGKSNSSSAAASAAAPISPSSTTNSTVGGGSRIGPDKGEACYRGNKWLPL 180

Query: 181 ETLVVGSFLLCFLLDSYHV 197
           E LV G+ LLCF LDS +V
Sbjct: 181 ENLVFGALLLCFFLDSLYV 199

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BLAST of Lag0002821 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 3.8e-49
Identity = 104/188 (55.32%), Postives = 129/188 (68.62%), Query Frame = 0


Query: 15  LLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCTGLKEVLKNNKKC 74
           L+    V   A D  KDKEECT+QL GMATCLPYV G AK+PTPDCC+GLK+VL +NKKC
Sbjct: 15  LIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKC 74

Query: 75  LCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPNSPEAQVFYQFGKA 134
           LCVI++DRNDPDLGLQINV+LAL LP VCHA A+V+ CPALL+L PNSP+AQVFYQ  K 
Sbjct: 75  LCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQLAKG 134

Query: 135 NSSSSAVAA------GPISPSPSSTVGEKSRTGL-----DKGEAFNLGKKWLPLETLVVG 192
            + +   +A      GPIS SP+S   + + +G       +  A +  K+WL LE  VV 
Sbjct: 135 LNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLE--VVF 194

[Close]

BLAST of Lag0002821 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 6.8e-46
Identity = 98/191 (51.31%), Postives = 131/191 (68.59%), Query Frame = 0


Query: 12  LSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCTGLKEVLKNN 71
           ++L++ +  V     D  KDKEECT QL GMATCLPYV G AK+PTPDCC+GLK+V+ ++
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPNSPEAQVFYQF 131
            KCLC+I+++RNDPDLGLQ+NV+LAL LP VCHA A+++ CPALL+L PNSP+AQVFYQ 
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQL 132

Query: 132 GK------ANSSSSAVAAGP--ISPSPSSTVGEKS-RTGLDKG--EAFNLGKKWLPLETL 191
            K      + S+ +  A+ P  +S +P S+ G  S RT    G   A +  K+WL LE  
Sbjct: 133 AKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLE-- 192

[Close]

BLAST of Lag0002821 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 9.5e-32
Identity = 70/177 (39.55%), Postives = 107/177 (60.45%), Query Frame = 0


Query: 10  LWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCTGLKEVLK 69
           L++++++TS  + F   D  +D+EECT QL  ++TC+PYV GDAK PT DCC G  +V++
Sbjct: 8   LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 67

Query: 70  NNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNCPALLNLPPNSPEAQVF 129
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C ++L+LP NS  A+ F
Sbjct: 68  KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 127

Query: 130 YQFGKANSSSSAVAAGPISPSPSSTVGEKSRTGLDKGEAFNLG----KKWLPLETLV 182
              G+   + +         S S T   K  TG  K E         K WL +E L+
Sbjct: 128 ENLGRIEDNYN---------STSPTQIHKDGTGGGKAEPVKSNGWKEKSWLGVELLI 175

[Close]

BLAST of Lag0002821 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 9.5e-24
Identity = 61/160 (38.12%), Postives = 90/160 (56.25%), Query Frame = 0


Query: 15  LLTSPAVSF--VAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCTGLKEVLK--N 74
           L  + AV F  V  +  +D + C   ++ + +CLP+V+  AK P   CC+ LK  +    
Sbjct: 16  LCITVAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDKGQ 75

Query: 75  NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPNSPEAQVFYQ 134
            +KCLC +VKDR+DP LG +++   A+ LP  CH PAN+S CP LL+L P+SP +Q+F Q
Sbjct: 76  TRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQIFKQ 135

Query: 135 FGKANSSSSAVAAGPISPSPSSTVGEKSRTGLDKGEAFNL 171
           F    SSS  V    +S S S    +  + GL    A ++
Sbjct: 136 F--TESSSQTVGHKAVSTSSSIKGRDNKQFGLMMAGALSI 173

[Close]

BLAST of Lag0002821 vs. ExPASy Swiss-Prot
Match: Q9C9B1 (Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3702 GN=LTPG10 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-12
Identity = 46/155 (29.68%), Postives = 69/155 (44.52%), Query Frame = 0


Query: 12  LSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCTGLKEVLKNN 71
           L  LL S +  F+     +    C  +L  +A C P+V G A+ P   CC  L ++    
Sbjct: 7   LITLLISLSAFFLRMVLAQVPATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQE 66

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNC-----------PALLNLPP 131
             CLC+ + + +       IN TLAL LP +C+ PAN S C            + +  PP
Sbjct: 67  ATCLCLFLNNTSTLSPAFPINQTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPP 126

Query: 132 NSPEAQVFYQFGKANSSSSAVAAGPISPSPSSTVG 156
           +S       Q  K NS  +A     ++P P+S +G
Sbjct: 127 SSSTGSQISQGAKNNSRVAATPVAQMAPRPTSFMG 161

[Close]

BLAST of Lag0002821 vs. ExPASy TrEMBL
Match: A0A6J1H6P3 (protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 7.2e-83
Identity = 159/195 (81.54%), Postives = 172/195 (88.21%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           M FHFA KWL L+LLL SPAVSF+AGDAEKD+EECTQQLAGMATCL YVSGDAK PTPDC
Sbjct: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C+GLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAVAAGPISPSP--SSTVGEKSRTGLDKGEAFNLGKKWLPLE 180
           +SPEAQVFYQFGKA SSS+  AA PISPSP  SST G  SR G DKGEA+ +G KWLPL+
Sbjct: 121 SSPEAQVFYQFGKAQSSSA--AAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLK 180

Query: 181 TLVVGSFLLCFLLDS 194
           +LVV + L+ FLLDS
Sbjct: 181 SLVVAALLVLFLLDS 193

[Close]

BLAST of Lag0002821 vs. ExPASy TrEMBL
Match: A0A6J1KZ76 (protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.1e-82
Identity = 158/198 (79.80%), Postives = 171/198 (86.36%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           M FHF  KWL L+LLL SPAVSF+AGDAEKD+EECTQQLAGMATCL YVSGDAK PTPDC
Sbjct: 1   MGFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C+GLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVS CPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAVAAGPISPSP--SSTVGEKSRTGLDKGEAFNLGKKWLPLE 180
           +SPEAQVFYQFGKA SSS+  AA PISPSP  +ST G  SR G DKGEA+ +G KWLPL+
Sbjct: 121 SSPEAQVFYQFGKAQSSSA--AAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLK 180

Query: 181 TLVVGSFLLCFLLDSYHV 197
           TL VG+ L+ FLLDS  V
Sbjct: 181 TLFVGALLVLFLLDSCRV 196

[Close]

BLAST of Lag0002821 vs. ExPASy TrEMBL
Match: A0A6J1D8S3 (protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.1e-82
Identity = 161/199 (80.90%), Postives = 170/199 (85.43%), Query Frame = 0


Query: 1   MDFHFAWKWLWLSLLLTSPAVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDC 60
           MDFHFA+K L L+LLLTSPAV  V  DAEKDKEECTQQLAGMATCLPYVSGDAK+PTPDC
Sbjct: 1   MDFHFAFKLLLLTLLLTSPAVRLVTADAEKDKEECTQQLAGMATCLPYVSGDAKSPTPDC 60

Query: 61  CTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C GLKEVLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPD CHA ANVSNCPALLNLPP
Sbjct: 61  CNGLKEVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDSCHASANVSNCPALLNLPP 120

Query: 121 NSPEAQVFYQFGKANSSSSAV-AAGPISPS--PSSTVGEKSRTGLDKGEAFNLGKKWLPL 180
           NSPEAQVFYQ GK+NSSS+A  AA PISPS   +STVG  SR G DKGEA   G KWLPL
Sbjct: 121 NSPEAQVFYQLGKSNSSSAAASAAAPISPSSTTNSTVGGGSRIGPDKGEACYRGNKWLPL 180

Query: 181 ETLVVGSFLLCFLLDSYHV 197
           E LV G+ LLCF LDS +V
Sbjct: 181 ENLVFGALLLCFFLDSLYV 199

[Close]

BLAST of Lag0002821 vs. ExPASy TrEMBL
Match: A0A1S3CDS0 (protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.1e-66
Identity = 134/189 (70.90%), Postives = 145/189 (76.72%), Query Frame = 0


Query: 3   FHFAWKWLWLSLLLTSP-AVSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCC 62
           F   +KW  L LLL SP +V FV GD +KDKEECT QLAGMATCLPYVSGDAKTPTPDCC
Sbjct: 4   FRLCFKWFLLMLLLRSPSSVRFVGGDDKKDKEECTPQLAGMATCLPYVSGDAKTPTPDCC 63

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPN 122
           +GLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPD+CHA ANVSNCPALLNLP N
Sbjct: 64  SGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLPSN 123

Query: 123 SPEAQVFYQFGKANSSSSAVAAGPISPSPSSTVGEKSRTGLDKGEAFNLGKKWLPLETLV 182
           S EAQVFYQ GK  SSS+   A  ISPS  +T+      G  K   + +GKKW+P E LV
Sbjct: 124 SSEAQVFYQLGKGKSSSALAPAPIISPSSPATISSTVGGGSTKSGGYKIGKKWVPSEILV 183

Query: 183 VGSFLLCFL 191
           V   LL  L
Sbjct: 184 VVVALLLLL 192

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BLAST of Lag0002821 vs. ExPASy TrEMBL
Match: A0A0A0K6P6 (Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.8e-62
Identity = 132/192 (68.75%), Postives = 152/192 (79.17%), Query Frame = 0


Query: 5   FAWKWLWL-SLLLTSPA-VSFVAGDAEKDKEECTQQLAGMATCLPYVSGDAKTPTPDCCT 64
           F++KWLWL  LLL SP+ V FV GD +KD+EECT QLAGMATCLPYVSGDAK PTPDCC+
Sbjct: 7   FSFKWLWLIMLLLRSPSWVRFVGGDDKKDREECTPQLAGMATCLPYVSGDAKAPTPDCCS 66

Query: 65  GLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPNS 124
           GLKEVL+N+KKCLCVIV+DRNDPDLGLQINVTLAL LPD+CHA ANVSNCPALLN+P NS
Sbjct: 67  GLKEVLQNDKKCLCVIVRDRNDPDLGLQINVTLALSLPDICHATANVSNCPALLNMPSNS 126

Query: 125 PEAQVFYQFGKANSSSSAVAAGPISPSPS----STVGEKSRTGLDKGEAFNLGKKWLPLE 184
            +AQVFYQ GK  SSS+   A  +SPS S    STVG  S++G + GE +  GKK + LE
Sbjct: 127 SDAQVFYQLGKGKSSSALAPAPMLSPSSSPTINSTVGGGSKSGAE-GEGYKRGKKRVALE 186

Query: 185 TLVVGSFLLCFL 191
            LVV + LL  L
Sbjct: 187 ILVVVAPLLLLL 197

The following BLAST results are available for this feature:

BLAST of Lag0002821 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899115.1	1.2e-87	83.67	non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida]	[more]
KAG6593339.1	1.0e-83	80.71	Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Prot...	[more]
XP_022959693.1	1.5e-82	81.54	protein YLS3-like [Cucurbita moschata]	[more]
XP_023513729.1	2.5e-82	81.03	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022150545.1	1.3e-81	80.90	protein YLS3-like [Momordica charantia]	[more]

BLAST of Lag0002821 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64865	3.8e-49	55.32	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
O64864	6.8e-46	51.31	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
F4I082	9.5e-32	39.55	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
Q9M2G1	9.5e-24	38.13	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9C9B1	2.2e-12	29.68	Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lag0002821 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6P3	7.2e-83	81.54	protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1	[more]
A0A6J1KZ76	6.1e-82	79.80	protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1	[more]
A0A6J1D8S3	6.1e-82	80.90	protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1	[more]
A0A1S3CDS0	2.1e-66	70.90	protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1	[more]
A0A0A0K6P6	1.8e-62	68.75	Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 35..112 e-value: 2.8E-5 score: 33.6
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 25..112 e-value: 3.5E-14 score: 52.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 32..112 e-value: 2.2E-14 score: 55.1
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 34..113
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 23..188
None	No IPR available	PANTHER	PTHR33044:SF192	PROTEIN YLS3-LIKE ISOFORM X1	coord: 23..188
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 42..104 e-value: 1.24622E-16 score: 68.9926

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002821.1	Lag0002821.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008643	carbohydrate transport
biological_process	GO:0006869	lipid transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008289	lipid binding
molecular_function	GO:0015293	symporter activity