Lag0002776 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002776 vs. NCBI nr
Match: XP_023515212.1 (protein DETOXIFICATION 43-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515213.1 protein DETOXIFICATION 43-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515214.1 protein DETOXIFICATION 43-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-262
Identity = 499/587 (85.01%), Postives = 528/587 (89.95%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFKFD IGREIL IALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKFDTIGREILEIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEED I +AATKAAKGDKG CLADD+SVKV+VPE+
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDAISQAATKAAKGDKGNCLADDHSVKVNVPEE 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPD-NGALQD 212
            + E+ EKLA KQ+SVN+NHEPT++ V+IEQGAGKENKESSST++GT+ES PD NGALQD
Sbjct: 121 HEREDSEKLAAKQDSVNMNHEPTKNIVSIEQGAGKENKESSSTKEGTEESGPDNNGALQD 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           L K+S  NV KST AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKS LNLMGVK
Sbjct: 181 LGKESGANVIKSTAAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSFLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIV GYTVNIILDPIL
Sbjct: 241 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVLGYTVNIILDPIL 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS+KDLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLKDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           +AVTFCVTLAA+LAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK+YE
Sbjct: 361 IAVTFCVTLAAALAARLGPIPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKNYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           K TATATRVLQMSFILGVGLAI VGIG FFGAGIFS+DIHVQYLIHLGIPFIAATQPINS
Sbjct: 421 KITATATRVLQMSFILGVGLAIFVGIGTFFGAGIFSKDIHVQYLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSLAS-------------------------FGVDCERRISV 572
           LAFVFDGVNFGASDFAYSAYSL S                          GV CER ISV
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSLVSSSGSSSTWLQNLLDRYSVCSSYASGSGVYCERCISV 540

Query: 573 SSLQKQWLHWDLDCFDNLYVSAYVCWCMEDGHRYRTLALPQDSHASL 594
           SSLQ+QWLH DL CF++L+VSAYVC  +EDG+R RT+ALPQD  ASL
Sbjct: 541 SSLQEQWLHRDLVCFNHLHVSAYVCGRLEDGYRNRTVALPQDPKASL 587

BLAST of Lag0002776 vs. NCBI nr
Match: KAG6575688.1 (Protein DETOXIFICATION 43, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 912.5 bits (2357), Expect = 1.9e-261
Identity = 498/592 (84.12%), Postives = 524/592 (88.51%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFK D IGREILGIALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKLDTIGREILGIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEE+TIGKA  KAA+ +KGKC ADDNSVK++VPED
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEETIGKADNKAARVEKGKCSADDNSVKIYVPED 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQ-D 212
             LE DE+L  KQ+SVNLNHEP RSN T EQ   KENKESSST++G KE VP+NGALQ D
Sbjct: 121 PDLENDERLTAKQDSVNLNHEP-RSNATTEQVGEKENKESSSTKEGKKELVPNNGALQED 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           LEKDSSTNV K+T AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 LEKDSSTNVIKATSAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPML PAHKYLTLRS+GAPAVLLSLAMQGIFRGFKDT+TPLYVIVSGYTVNIILDPI 
Sbjct: 241 DNSPMLVPAHKYLTLRSIGAPAVLLSLAMQGIFRGFKDTKTPLYVIVSGYTVNIILDPIF 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS++DLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLRDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           VAVTFCVTLAASLAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQA+LASAFAEKDYE
Sbjct: 361 VAVTFCVTLAASLAARLGPKPMAAFQTCLQVWMTSSLLSDGLAVAGQALLASAFAEKDYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIP---------- 512
           KTTATATRVLQMSFILGVGLA+ VGIGMFFGAGIFSRDI VQYLIHLGIP          
Sbjct: 421 KTTATATRVLQMSFILGVGLAVFVGIGMFFGAGIFSRDIQVQYLIHLGIPVNIKTYLVFF 480

Query: 513 -------------------FIAATQPINSLAFVFDGVNFGASDFAYSAYSLASFGVDCER 572
                              FIAATQPINSLAFVFDGVNFGASDFAYSAYSLAS G DCER
Sbjct: 481 LPMPWMNSSDSPLSPETHQFIAATQPINSLAFVFDGVNFGASDFAYSAYSLASSGGDCER 540

Query: 573 RISVSSLQKQWLHWDLDCFDNLYVSAYVCWCMEDGHRYRTLALPQDSHASLS 595
            I+VSSLQKQWLH DLDCFD+L+ SAY CW +EDGH  RTLAL Q+  A+L+
Sbjct: 541 CITVSSLQKQWLHRDLDCFDDLHASAYFCWHLEDGHGNRTLALSQNPKATLN 591

BLAST of Lag0002776 vs. NCBI nr
Match: KAG6593302.1 (Protein DETOXIFICATION 43, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 869.0 bits (2244), Expect = 2.4e-248
Identity = 471/527 (89.37%), Postives = 499/527 (94.69%), Query Frame = 0

Query: 10  STLANQNQNQNLNLTTKGVVKSKMPVNVFFKDARRVFKFDAIGREILGIALPAALAVAAD 69
           ST   QNQNQNL+LTT+G +K KMPVNVFFKDARRVFKFD IGREIL IALPAALAVAAD
Sbjct: 28  STTILQNQNQNLSLTTRG-IKPKMPVNVFFKDARRVFKFDTIGREILEIALPAALAVAAD 87

Query: 70  PVASLIDTAFVGHIGPVELAAVGVSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATK 129
           PVASLIDTAFVGH+GPVELAAVGVSIAIFNQASRITIFPLVSITTSFVAEED I +AATK
Sbjct: 88  PVASLIDTAFVGHLGPVELAAVGVSIAIFNQASRITIFPLVSITTSFVAEEDAISQAATK 147

Query: 130 AAKGDKGKCLADDNSVKVHVPEDRKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKEN 189
           AAKGDKGKCLADD+SVKV+VPE+ + E+ EKLA KQ+SVN+NHEPT++ V+IEQGAGKEN
Sbjct: 148 AAKGDKGKCLADDHSVKVNVPEEHEREDSEKLAAKQDSVNMNHEPTKNIVSIEQGAGKEN 207

Query: 190 KESSSTEKGTKESVP-DNGALQDLEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSI 249
           KESSST++GT+E+ P +NGALQDL  +S  NV KST AKSKKKEKKQIASASTALIFGSI
Sbjct: 208 KESSSTKEGTEETGPGNNGALQDLGTESGANVIKSTAAKSKKKEKKQIASASTALIFGSI 267

Query: 250 LGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFK 309
           LGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYLTLRS+GAPAVLLSLAMQGIFRGFK
Sbjct: 268 LGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYLTLRSIGAPAVLLSLAMQGIFRGFK 327

Query: 310 DTRTPLYVIVSGYTVNIILDPILIFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLM 369
           DTRTPLYVIV GYTVNIILDPILIFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLM
Sbjct: 328 DTRTPLYVIVLGYTVNIILDPILIFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLM 387

Query: 370 PPSIKDLQFGRFLKNGGLLLARVVAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSL 429
           PPS+KDLQFGRFLKNGGLLLARV+AVTFCVTLAA+LAARLGP PMAAFQTCLQVWMTSSL
Sbjct: 388 PPSLKDLQFGRFLKNGGLLLARVIAVTFCVTLAAALAARLGPIPMAAFQTCLQVWMTSSL 447

Query: 430 LSDGLAVAGQAILASAFAEKDYEKTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSR 489
           LSDGLAVAGQAILASAFAEK+YEK TATATRVLQMSFILGVGLAI VGIG FFGAGIFS+
Sbjct: 448 LSDGLAVAGQAILASAFAEKNYEKATATATRVLQMSFILGVGLAIFVGIGTFFGAGIFSK 507

Query: 490 DIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGASDFAYSAYSLAS 536
           DIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGASDFAYSAYSL S
Sbjct: 508 DIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGASDFAYSAYSLVS 553

BLAST of Lag0002776 vs. NCBI nr
Match: KAG6593302.1 (Protein DETOXIFICATION 43, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 60.8 bits (146), Expect = 4.5e-05
Identity = 31/47 (65.96%), Postives = 38/47 (80.85%), Query Frame = 0

Query: 529 SAYSLASFGVDCERRISVSSLQKQWLHWDLDCFDNLYVSAYVCWCME 576
           S+Y+  S GV CER ISVSSLQ+QWLH DL CF++L+VSAYVC  +E
Sbjct: 573 SSYASGS-GVYCERCISVSSLQEQWLHRDLVCFNHLHVSAYVCGHLE 618

HSP 2 Score: 864.0 bits (2231), Expect = 7.6e-247
Identity = 469/502 (93.43%), Postives = 483/502 (96.22%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADP+ASLIDTAFVGHIGPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPIASLIDTAFVGHIGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAA KAAK D  KCLADDNSVKV VPED
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAANKAAKVDTQKCLADDNSVKVSVPED 120

Query: 153 R-KLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQD 212
           R +LE DEKLA KQ+ VNLNHEPTRSN+TIE+G GKENK+SSST+ GTKE VPDNGALQD
Sbjct: 121 RDQLENDEKLAAKQDHVNLNHEPTRSNITIEKG-GKENKQSSSTKMGTKEPVPDNGALQD 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
            EKD STNV KST AKSK+KEKKQIASASTALIFG+ILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 PEKDLSTNVLKSTSAKSKRKEKKQIASASTALIFGTILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPM APAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLY+IV+GYTVNIILDPI 
Sbjct: 241 DNSPMFAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYIIVAGYTVNIILDPIF 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIV+ILFWRL+QKVNLMPPS+KDLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVIILFWRLMQKVNLMPPSLKDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE
Sbjct: 361 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQ LIHLGIPFIAATQPINS
Sbjct: 421 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQNLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSL 534
           LAFVFDGVNFGASDFAYSAYSL
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSL 501

BLAST of Lag0002776 vs. NCBI nr
Match: XP_022959712.1 (protein DETOXIFICATION 43 isoform X1 [Cucurbita moschata] >XP_022959713.1 protein DETOXIFICATION 43 isoform X1 [Cucurbita moschata] >XP_022959714.1 protein DETOXIFICATION 43 isoform X1 [Cucurbita moschata] >XP_022959716.1 protein DETOXIFICATION 43 isoform X1 [Cucurbita moschata])

HSP 1 Score: 848.6 bits (2191), Expect = 3.3e-242
Identity = 457/504 (90.67%), Postives = 482/504 (95.63%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFKFD IGREIL IALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKFDTIGREILEIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEED I +AATKAAKGDKGKCLADD+SVKV+VPE+
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDAISQAATKAAKGDKGKCLADDHSVKVNVPEE 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPD-NGALQD 212
            + E+ EKLA KQ+SVN+NHEPT++ V+IEQGAGKENKESSST++GT+E+ PD NGALQD
Sbjct: 121 HEREDSEKLAAKQDSVNMNHEPTKNIVSIEQGAGKENKESSSTKEGTEETGPDNNGALQD 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           L K+S  NV KST AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 LGKESGANVIKSTAAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPMLAPAHKYLTLRS+GAPAVLLSLAMQGIFRGFKDTRTPLYVIV GYTVNIILDPIL
Sbjct: 241 DNSPMLAPAHKYLTLRSIGAPAVLLSLAMQGIFRGFKDTRTPLYVIVLGYTVNIILDPIL 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS+KDLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLKDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           +AVTFCVTLAA+LAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK+YE
Sbjct: 361 IAVTFCVTLAAALAARLGPIPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKNYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           K TATATRVLQMSFILGVGLAI VGIG FFGAGIFS+DIHVQ+LIHLGIPFIAATQPINS
Sbjct: 421 KATATATRVLQMSFILGVGLAIFVGIGTFFGAGIFSKDIHVQHLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSLAS 536
           LAFVFDGVNFGASDFAYSAYSL S
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSLVS 504

BLAST of Lag0002776 vs. ExPASy Swiss-Prot
Match: Q9SFB0 (Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.5e-152
Identity = 309/505 (61.19%), Postives = 371/505 (73.47%), Query Frame = 0

Query: 29  VKSKMPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVEL 88
           VK  +P  V FKD R VF  D  GREILGIA PAALA+AADP+ASLIDTAFVG +G V+L
Sbjct: 11  VKKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQL 70

Query: 89  AAVGVSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVH 148
           AAVGVSIAIFNQASRITIFPLVS+TTSFVAEEDT+                         
Sbjct: 71  AAVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTM------------------------- 130

Query: 149 VPEDRKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGA 208
                     EK+  + N  NL H  T   + ++           S EKG   S P +  
Sbjct: 131 ----------EKMKEEANKANLVHAET---ILVQ----------DSLEKGI--SSPTSND 190

Query: 209 LQDLEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLM 268
               ++  + +   ++  KS KKEK+ I +ASTA+I G ILGL+QAIFL+F +K LL +M
Sbjct: 191 TNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLLLGVM 250

Query: 269 GVKDNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILD 328
           GVK NSPML+PAHKYL++R+LGAPA+LLSLAMQGIFRGFKDT+TPL+  V    +NI+LD
Sbjct: 251 GVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLD 310

Query: 329 PILIFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLL 388
           PI IFV R G+ GAA AHV+SQYF+ LILF  L +KVNL+PP+  DLQFGRFLKNG LLL
Sbjct: 311 PIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLKNGLLLL 370

Query: 389 ARVVAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK 448
           AR +AVTFC TLAA++AARLG TPMAAFQ CLQVW+TSSLL+DGLAVAGQAILA +FAEK
Sbjct: 371 ARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILACSFAEK 430

Query: 449 DYEKTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQP 508
           DY K TA A+RVLQM F+LG+GL++ VG+G++FGAG+FS+D  V +L+ +GIPFIAATQP
Sbjct: 431 DYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPFIAATQP 465

Query: 509 INSLAFVFDGVNFGASDFAYSAYSL 534
           INSLAFV DGVNFGASDFAY+AYS+
Sbjct: 491 INSLAFVLDGVNFGASDFAYTAYSM 465

BLAST of Lag0002776 vs. ExPASy Swiss-Prot
Match: Q9SYD6 (Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2)

HSP 1 Score: 475.7 bits (1223), Expect = 7.6e-133
Identity = 280/505 (55.45%), Postives = 353/505 (69.90%), Query Frame = 0

Query: 34  PVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVGV 93
           P+ +FF D R V KFD +G EI  IALPAALA+ ADP+ASL+DTAF+G IGPVELAAVGV
Sbjct: 15  PLYIFFSDFRSVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGV 74

Query: 94  SIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPEDR 153
           SIA+FNQ SRI IFPLVSITTSFVAEED                C +  ++V+       
Sbjct: 75  SIALFNQVSRIAIFPLVSITTSFVAEEDA---------------CSSQQDTVR------- 134

Query: 154 KLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDLE 213
               D K  ++   + +N+ PT   + +     +++K+S S E  T  S+          
Sbjct: 135 ----DHKECIE---IGINN-PTEETIEL---IPEKHKDSLSDEFKTSSSI---------- 194

Query: 214 KDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKDN 273
                +++K  PAK     K+ I SAS+ALI G +LGL QA+FL+  AK LL+ MGVK +
Sbjct: 195 ----FSISK-PPAK-----KRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHD 254

Query: 274 SPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIF 333
           SPM+ P+ +YL+LRSLGAPAVLLSLA QG+FRGFKDT TPL+  V G   NIILDPI IF
Sbjct: 255 SPMMRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIF 314

Query: 334 VCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVVA 393
           V R GV GAA AHV+SQY +  IL W+L+ +V++   S K LQF RF+KNG LLL RV+A
Sbjct: 315 VFRLGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIA 374

Query: 394 VTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEKT 453
           VTFCVTL+ASLAAR G T MAAFQ CLQVW+ +SLL+DG AVAGQAILASAFA+KDY++ 
Sbjct: 375 VTFCVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRA 434

Query: 454 TATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSLA 513
            ATA+RVLQ+  +LG  LA+++G G+ FGA +F++D  V +LI +G+PF+A TQPIN+LA
Sbjct: 435 AATASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALA 466

Query: 514 FVFDGVNFGASDFAYSAYSLASFGV 539
           FVFDGVNFGASDF Y+A SL    +
Sbjct: 495 FVFDGVNFGASDFGYAAASLVMVAI 466

BLAST of Lag0002776 vs. ExPASy Swiss-Prot
Match: Q84K71 (Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX44 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.8e-81
Identity = 192/487 (39.43%), Postives = 272/487 (55.85%), Query Frame = 0

Query: 51  IGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVGVSIAIFNQASRITIFPLV 110
           IG EI+ IALPAALA+AADP+ SL+DTAFVGHIG  ELAAVGVS+++FN  S++   PL+
Sbjct: 76  IGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPLL 135

Query: 111 SITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPEDRKLEEDEKLAVKQNSVNL 170
           ++TTSFVAEE  I      AAK        DDN                           
Sbjct: 136 NVTTSFVAEEQAI------AAK--------DDND-------------------------- 195

Query: 171 NHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDLEKDSSTNVTKSTPAKSKK 230
                                                                    S +
Sbjct: 196 ---------------------------------------------------------SIE 255

Query: 231 KEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYLTLRSLG 290
             KK + S ST+L+  + +G+ +AI L  G+  L+++M +  +SPM  PA ++L LR+ G
Sbjct: 256 TSKKVLPSVSTSLVLAAGVGIAEAIALSLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYG 315

Query: 291 APAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIFVCRWGVKGAAAAHVLSQ 350
           AP ++++LA QG FRGFKDT TPLY +V+G  +N +LDPILIFV  +G+ GAAAA V+S+
Sbjct: 316 APPIVVALAAQGAFRGFKDTTTPLYAVVAGNVLNAVLDPILIFVLGFGISGAAAATVISE 375

Query: 351 YFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVVAVTFCVTLAASLAARLGP 410
           Y I  IL W+L + V L+ P IK  +  ++LK+GGLL+ R VA+    TLA SLAA+ GP
Sbjct: 376 YLIAFILLWKLNENVVLLSPQIKVGRANQYLKSGGLLIGRTVALLVPFTLATSLAAQNGP 435

Query: 411 TPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEKTTATATRVLQMSFILGVG 470
           T MA  Q  L++W+  SLL+D LA+A Q++LA+ +++ +Y++       VLQ+    G G
Sbjct: 436 TQMAGHQIVLEIWLAVSLLTDALAIAAQSLLATTYSQGEYKQAREVLFGVLQVGLATGTG 465

Query: 471 LAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGASDFAYSA 530
           LA V+ I     + +F+ D  V  +   G  F+A +QP+N+LAFV DG+ +G SDF ++A
Sbjct: 496 LAAVLFITFEPFSSLFTTDSEVLKIALSGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAA 465

Query: 531 YSLASFG 538
           YS+   G
Sbjct: 556 YSMVIVG 465

BLAST of Lag0002776 vs. ExPASy Swiss-Prot
Match: Q9SVE7 (Protein DETOXIFICATION 45, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX45 PE=2 SV=2)

HSP 1 Score: 286.2 bits (731), Expect = 8.5e-76
Identity = 180/487 (36.96%), Postives = 274/487 (56.26%), Query Frame = 0

Query: 51  IGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVGVSIAIFNQASRITIFPLV 110
           I RE++ ++LPA    A DP+  L++TA++G +G VEL + GVS+AIFN  S++   PL+
Sbjct: 105 IKRELVMLSLPAIAGQAIDPLTLLMETAYIGRLGSVELGSAGVSMAIFNTISKLFNIPLL 164

Query: 111 SITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPEDRKLEEDEKLAVKQNSVNL 170
           S+ TSFVAE+  I K A +         LA ++S                          
Sbjct: 165 SVATSFVAED--IAKIAAQD--------LASEDS-------------------------- 224

Query: 171 NHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDLEKDSSTNVTKSTPAKSKK 230
                                        +  +P  G                       
Sbjct: 225 -----------------------------QSDIPSQGL---------------------- 284

Query: 231 KEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYLTLRSLG 290
            E+KQ++S STAL+    +G+ +A+ L   +   L LMG++  S M  PA ++L LR+LG
Sbjct: 285 PERKQLSSVSTALVLAIGIGIFEALALSLASGPFLRLMGIQSMSEMFIPARQFLVLRALG 344

Query: 291 APAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIFVCRWGVKGAAAAHVLSQ 350
           APA ++SLA+QGIFRGFKDT+TP+Y +  G  + + L P+ I+  R GV GAA + V+SQ
Sbjct: 345 APAYVVSLALQGIFRGFKDTKTPVYCLGIGNFLAVFLFPLFIYKFRMGVAGAAISSVISQ 404

Query: 351 YFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVVAVTFCVTLAASLAARLGP 410
           Y + +++   L ++V L+PP I  L+FG +LK+GG +L R ++V   +T+A S+AAR G 
Sbjct: 405 YTVAILMLILLNKRVILLPPKIGSLKFGDYLKSGGFVLGRTLSVLVTMTVATSMAARQGV 464

Query: 411 TPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEKTTATATRVLQMSFILGVG 470
             MAA Q C+QVW+  SLL+D LA +GQA++AS+ +++D+E      T VL++  + G+ 
Sbjct: 465 FAMAAHQICMQVWLAVSLLTDALASSGQALIASSASKRDFEGVKEVTTFVLKIGVVTGIA 504

Query: 471 LAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGASDFAYSA 530
           LAIV+G+     AG+FS+D  V  ++  G+ F+AATQPI +LAF+FDG+++G SDF Y+A
Sbjct: 525 LAIVLGMSFSSIAGLFSKDPEVLRIVRKGVLFVAATQPITALAFIFDGLHYGMSDFPYAA 504

Query: 531 YSLASFG 538
            S+   G
Sbjct: 585 CSMMVVG 504

BLAST of Lag0002776 vs. ExPASy Swiss-Prot
Match: Q8W4G3 (Protein DETOXIFICATION 46, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX46 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 9.0e-09
Identity = 88/330 (26.67%), Postives = 149/330 (45.15%), Query Frame = 0

Query: 216 SSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGL-MQAIFLVFGAKSLLNLMGVKDNS 275
           +++N+  ++ A+  K E +     S  L  G   G+ M  +  +FG+ +L    GVK N+
Sbjct: 175 ATSNLVATSLARQDKDEVQH--QISILLFIGLACGVTMMVLTRLFGSWALTAFTGVK-NA 234

Query: 276 PMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIFV 335
            ++  A+KY+ +R L  PAVL+    Q    G KD+  PL  +     +N + D +L   
Sbjct: 235 DIVPAANKYVQIRGLAWPAVLIGWVAQSASLGMKDSWGPLKALAVASAINGVGDVVLCTF 294

Query: 336 CRWGVKGAAAAHVLSQYFIVLILFWRLVQK----VNLMPPSIKDLQ--FGRFLKNGGLLL 395
             +G+ GAA A ++SQ     ++   L +K     +   PS  +L   FG        ++
Sbjct: 295 LGYGIAGAAWATMVSQVVAAYMMMDALNKKGYSAFSFCVPSPSELLTIFGLAAPVFITMM 354

Query: 396 ARVVAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK 455
           ++V+  T  V  A S    +G   +AA Q  LQ++  S++  + L+   Q+ +       
Sbjct: 355 SKVLFYTLLVYFATS----MGTNIIAAHQVMLQIYTMSTVWGEPLSQTAQSFMPELLF-- 414

Query: 456 DYEKTTATATRVLQMSFILGVGLAIVVG-IGM---FFGAGIFSRDIHVQYLIH-LGIPFI 515
              +    A  +L+   I+G  L IVVG IG    +   GIF+RD  V   +H + IP+ 
Sbjct: 415 GINRNLPKARVLLKSLVIIGATLGIVVGTIGTAVPWLFPGIFTRDKVVTSEMHKVIIPYF 474

Query: 516 AATQPINSLAFVFDGVNFGASDFAYSAYSL 534
            A   I       +G      D  Y + S+
Sbjct: 475 LALS-ITPSTHSLEGTLLAGRDLRYISLSM 494

BLAST of Lag0002776 vs. ExPASy TrEMBL
Match: A0A6J1KZR7 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111497792 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.6e-242
Identity = 463/518 (89.38%), Postives = 487/518 (94.02%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFKFD IGREIL IALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKFDTIGREILEIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEED I +AATKAAKGDKGK LADD+SVKV+VPE+
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDAISQAATKAAKGDKGKRLADDHSVKVNVPEE 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPD-NGALQD 212
            + E+ EKLA KQ+SVN+NHEPT+S V+IEQGAGKENKESSST++GT+E+ PD NGALQD
Sbjct: 121 HESEDSEKLAAKQDSVNMNHEPTKSIVSIEQGAGKENKESSSTKEGTEETGPDNNGALQD 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           L K+S  NV KST AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 LGKESGANVIKSTAAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIV GYTVNIILDPIL
Sbjct: 241 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVLGYTVNIILDPIL 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS+KDLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLKDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           +AVTFCVTLAA+LAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK+YE
Sbjct: 361 IAVTFCVTLAAALAARLGPIPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKNYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           KTTATATRVLQMSFILGVGLAI VGIG FFGAGIFS+DIHVQYLIHLGIPFIAATQPINS
Sbjct: 421 KTTATATRVLQMSFILGVGLAIFVGIGTFFGAGIFSKDIHVQYLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSLASFGVDCERRISVSSL 550
           LAFVFDGVNFGASDFAYSAYSL    +     +SV SL
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSLVLVSI-----VSVVSL 513

BLAST of Lag0002776 vs. ExPASy TrEMBL
Match: A0A6J1H8W7 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111460701 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.6e-242
Identity = 457/504 (90.67%), Postives = 482/504 (95.63%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFKFD IGREIL IALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKFDTIGREILEIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEED I +AATKAAKGDKGKCLADD+SVKV+VPE+
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDAISQAATKAAKGDKGKCLADDHSVKVNVPEE 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPD-NGALQD 212
            + E+ EKLA KQ+SVN+NHEPT++ V+IEQGAGKENKESSST++GT+E+ PD NGALQD
Sbjct: 121 HEREDSEKLAAKQDSVNMNHEPTKNIVSIEQGAGKENKESSSTKEGTEETGPDNNGALQD 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           L K+S  NV KST AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 LGKESGANVIKSTAAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPMLAPAHKYLTLRS+GAPAVLLSLAMQGIFRGFKDTRTPLYVIV GYTVNIILDPIL
Sbjct: 241 DNSPMLAPAHKYLTLRSIGAPAVLLSLAMQGIFRGFKDTRTPLYVIVLGYTVNIILDPIL 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS+KDLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLKDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           +AVTFCVTLAA+LAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK+YE
Sbjct: 361 IAVTFCVTLAAALAARLGPIPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKNYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           K TATATRVLQMSFILGVGLAI VGIG FFGAGIFS+DIHVQ+LIHLGIPFIAATQPINS
Sbjct: 421 KATATATRVLQMSFILGVGLAIFVGIGTFFGAGIFSKDIHVQHLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSLAS 536
           LAFVFDGVNFGASDFAYSAYSL S
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSLVS 504

BLAST of Lag0002776 vs. ExPASy TrEMBL
Match: A0A6J1H8W7 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111460701 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-12
Identity = 43/65 (66.15%), Postives = 52/65 (80.00%), Query Frame = 0

Query: 529 SAYSLASFGVDCERRISVSSLQKQWLHWDLDCFDNLYVSAYVCWCMEDGHRYRTLALPQD 588
           S+Y+  S GV CER ISVSSLQ+QWLH DL CF++L+VSAYVC  +EDGHR  T+ALP+D
Sbjct: 524 SSYASGS-GVYCERCISVSSLQEQWLHRDLVCFNHLHVSAYVCGHLEDGHRNGTVALPED 583

Query: 589 SHASL 594
             ASL
Sbjct: 584 PKASL 587

HSP 2 Score: 847.4 bits (2188), Expect = 3.5e-242
Identity = 460/518 (88.80%), Postives = 487/518 (94.02%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFKFD IGREIL IALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKFDTIGREILEIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEED I +AATKAAKGDKGKCLADD+SVKV+VPE+
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDAISQAATKAAKGDKGKCLADDHSVKVNVPEE 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPD-NGALQD 212
            + E+ EKLA KQ+SVN+NHEPT++ V+IEQGAGKENKESSST++GT+E+ PD NGALQD
Sbjct: 121 HEREDSEKLAAKQDSVNMNHEPTKNIVSIEQGAGKENKESSSTKEGTEETGPDNNGALQD 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           L K+S  NV KST AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 LGKESGANVIKSTAAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPMLAPAHKYLTLRS+GAPAVLLSLAMQGIFRGFKDTRTPLYVIV GYTVNIILDPIL
Sbjct: 241 DNSPMLAPAHKYLTLRSIGAPAVLLSLAMQGIFRGFKDTRTPLYVIVLGYTVNIILDPIL 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS+KDLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLKDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           +AVTFCVTLAA+LAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK+YE
Sbjct: 361 IAVTFCVTLAAALAARLGPIPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKNYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           K TATATRVLQMSFILGVGLAI VGIG FFGAGIFS+DIHVQ+LIHLGIPFIAATQPINS
Sbjct: 421 KATATATRVLQMSFILGVGLAIFVGIGTFFGAGIFSKDIHVQHLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSLASFGVDCERRISVSSL 550
           LAFVFDGVNFGASDFAYSAYSL    +     +SV SL
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSLVLVSI-----VSVVSL 513

BLAST of Lag0002776 vs. ExPASy TrEMBL
Match: A0A1S3CED3 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103499923 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 2.2e-239
Identity = 453/501 (90.42%), Postives = 470/501 (93.81%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MP+NVFFKDARRVFK+D IGREILGIALPAALAVAADP+ASLIDTAFVGHIGPVELAAVG
Sbjct: 1   MPINVFFKDARRVFKYDEIGREILGIALPAALAVAADPIASLIDTAFVGHIGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAA KAAK D  KCL DDNSVKV+V ED
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAAKKAAKVDTEKCLDDDNSVKVYVSED 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDL 212
              E +EKLA KQ   NLNHEPTR N++I +   KENKESSSTE GTKE +PDNGALQDL
Sbjct: 121 H--ENEEKLAAKQEHANLNHEPTRINISIGKDGVKENKESSSTENGTKEPIPDNGALQDL 180

Query: 213 EKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKD 272
           EKD ST V KST AKSK+KEKKQIASASTALIFG+ILGLMQAIFL+FGAKSLLNLMGVKD
Sbjct: 181 EKDLSTKVLKSTSAKSKRKEKKQIASASTALIFGTILGLMQAIFLIFGAKSLLNLMGVKD 240

Query: 273 NSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILI 332
           NSPM APAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIV+GYTVNIILDPILI
Sbjct: 241 NSPMFAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVAGYTVNIILDPILI 300

Query: 333 FVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVV 392
           FVC WGVKGAAAAHVLSQYFIV ILFWRLVQKVNLMPPS+KDLQFGRFLKNGGLLLARVV
Sbjct: 301 FVCHWGVKGAAAAHVLSQYFIVTILFWRLVQKVNLMPPSLKDLQFGRFLKNGGLLLARVV 360

Query: 393 AVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEK 452
           AVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEK
Sbjct: 361 AVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEK 420

Query: 453 TTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSL 512
           TTATATRVLQMSFILGV LAI+VGIGMFFGAGIFSRDIHVQ+LIHL IPF+AATQPINSL
Sbjct: 421 TTATATRVLQMSFILGVTLAIIVGIGMFFGAGIFSRDIHVQHLIHLAIPFVAATQPINSL 480

Query: 513 AFVFDGVNFGASDFAYSAYSL 534
           AFVFDGVNFGASDFAYSAYSL
Sbjct: 481 AFVFDGVNFGASDFAYSAYSL 499

BLAST of Lag0002776 vs. ExPASy TrEMBL
Match: A0A6J1GS75 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111456521 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 1.1e-238
Identity = 459/518 (88.61%), Postives = 480/518 (92.66%), Query Frame = 0

Query: 33  MPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVG 92
           MPVNVFFKDARRVFK D IGREILGIALPAALAVAADPVASLIDTAFVGH+GPVELAAVG
Sbjct: 1   MPVNVFFKDARRVFKLDTIGREILGIALPAALAVAADPVASLIDTAFVGHLGPVELAAVG 60

Query: 93  VSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPED 152
           VSIAIFNQASRITIFPLVSITTSFVAEE+TIGKA  KAA+ +KGK  ADDNSVKV+VPED
Sbjct: 61  VSIAIFNQASRITIFPLVSITTSFVAEEETIGKADNKAARVEKGKGSADDNSVKVYVPED 120

Query: 153 RKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQ-D 212
             LE DE+L  KQ+SVNLNHEP RS+ T EQ   KENKESSST++G KE VP+NGALQ D
Sbjct: 121 PDLENDERLTAKQDSVNLNHEP-RSSATTEQVGEKENKESSSTKEGKKELVPNNGALQED 180

Query: 213 LEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 272
           LEKDSSTNV K+T AKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK
Sbjct: 181 LEKDSSTNVIKATSAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVK 240

Query: 273 DNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPIL 332
           DNSPML PAHKYLTLRS+GAPAVLLSLAMQGIFRGFKDT+TPLYVIVSGYTVNIILDPI 
Sbjct: 241 DNSPMLVPAHKYLTLRSIGAPAVLLSLAMQGIFRGFKDTKTPLYVIVSGYTVNIILDPIF 300

Query: 333 IFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARV 392
           IFVCRWGVKGAAAAHVLSQYFIVL+LFWRLVQKVNLMPPS++DLQFGRFLKNGGLLLARV
Sbjct: 301 IFVCRWGVKGAAAAHVLSQYFIVLVLFWRLVQKVNLMPPSLRDLQFGRFLKNGGLLLARV 360

Query: 393 VAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYE 452
           VAVTFCVTLAASLAARLGP PMAAFQTCLQVWMTSSLLSDGLAVAGQA+LASAFAEKDYE
Sbjct: 361 VAVTFCVTLAASLAARLGPKPMAAFQTCLQVWMTSSLLSDGLAVAGQALLASAFAEKDYE 420

Query: 453 KTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINS 512
           KTTATATRVLQMSFILGVGLA+ VGIGMFFGAGIFSRDI VQYLIHLGIPFIAATQPINS
Sbjct: 421 KTTATATRVLQMSFILGVGLAVFVGIGMFFGAGIFSRDIQVQYLIHLGIPFIAATQPINS 480

Query: 513 LAFVFDGVNFGASDFAYSAYSLASFGVDCERRISVSSL 550
           LAFVFDGVNFGASDFAYSAYSL    +     +SV SL
Sbjct: 481 LAFVFDGVNFGASDFAYSAYSLVMVAI-----VSVVSL 512

BLAST of Lag0002776 vs. TAIR 10
Match: AT3G08040.1 (MATE efflux family protein )

HSP 1 Score: 541.2 bits (1393), Expect = 1.0e-153
Identity = 309/505 (61.19%), Postives = 371/505 (73.47%), Query Frame = 0

Query: 29  VKSKMPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVEL 88
           VK  +P  V FKD R VF  D  GREILGIA PAALA+AADP+ASLIDTAFVG +G V+L
Sbjct: 11  VKKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQL 70

Query: 89  AAVGVSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVH 148
           AAVGVSIAIFNQASRITIFPLVS+TTSFVAEEDT+                         
Sbjct: 71  AAVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTM------------------------- 130

Query: 149 VPEDRKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGA 208
                     EK+  + N  NL H  T   + ++           S EKG   S P +  
Sbjct: 131 ----------EKMKEEANKANLVHAET---ILVQ----------DSLEKGI--SSPTSND 190

Query: 209 LQDLEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLM 268
               ++  + +   ++  KS KKEK+ I +ASTA+I G ILGL+QAIFL+F +K LL +M
Sbjct: 191 TNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLLLGVM 250

Query: 269 GVKDNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILD 328
           GVK NSPML+PAHKYL++R+LGAPA+LLSLAMQGIFRGFKDT+TPL+  V    +NI+LD
Sbjct: 251 GVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLD 310

Query: 329 PILIFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLL 388
           PI IFV R G+ GAA AHV+SQYF+ LILF  L +KVNL+PP+  DLQFGRFLKNG LLL
Sbjct: 311 PIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLKNGLLLL 370

Query: 389 ARVVAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK 448
           AR +AVTFC TLAA++AARLG TPMAAFQ CLQVW+TSSLL+DGLAVAGQAILA +FAEK
Sbjct: 371 ARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILACSFAEK 430

Query: 449 DYEKTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQP 508
           DY K TA A+RVLQM F+LG+GL++ VG+G++FGAG+FS+D  V +L+ +GIPFIAATQP
Sbjct: 431 DYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPFIAATQP 465

Query: 509 INSLAFVFDGVNFGASDFAYSAYSL 534
           INSLAFV DGVNFGASDFAY+AYS+
Sbjct: 491 INSLAFVLDGVNFGASDFAYTAYSM 465

BLAST of Lag0002776 vs. TAIR 10
Match: AT3G08040.2 (MATE efflux family protein )

HSP 1 Score: 541.2 bits (1393), Expect = 1.0e-153
Identity = 309/505 (61.19%), Postives = 371/505 (73.47%), Query Frame = 0

Query: 29  VKSKMPVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVEL 88
           VK  +P  V FKD R VF  D  GREILGIA PAALA+AADP+ASLIDTAFVG +G V+L
Sbjct: 11  VKKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQL 70

Query: 89  AAVGVSIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVH 148
           AAVGVSIAIFNQASRITIFPLVS+TTSFVAEEDT+                         
Sbjct: 71  AAVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTM------------------------- 130

Query: 149 VPEDRKLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGA 208
                     EK+  + N  NL H  T   + ++           S EKG   S P +  
Sbjct: 131 ----------EKMKEEANKANLVHAET---ILVQ----------DSLEKGI--SSPTSND 190

Query: 209 LQDLEKDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLM 268
               ++  + +   ++  KS KKEK+ I +ASTA+I G ILGL+QAIFL+F +K LL +M
Sbjct: 191 TNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLLLGVM 250

Query: 269 GVKDNSPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILD 328
           GVK NSPML+PAHKYL++R+LGAPA+LLSLAMQGIFRGFKDT+TPL+  V    +NI+LD
Sbjct: 251 GVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLD 310

Query: 329 PILIFVCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLL 388
           PI IFV R G+ GAA AHV+SQYF+ LILF  L +KVNL+PP+  DLQFGRFLKNG LLL
Sbjct: 311 PIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLKNGLLLL 370

Query: 389 ARVVAVTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEK 448
           AR +AVTFC TLAA++AARLG TPMAAFQ CLQVW+TSSLL+DGLAVAGQAILA +FAEK
Sbjct: 371 ARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILACSFAEK 430

Query: 449 DYEKTTATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQP 508
           DY K TA A+RVLQM F+LG+GL++ VG+G++FGAG+FS+D  V +L+ +GIPFIAATQP
Sbjct: 431 DYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPFIAATQP 465

Query: 509 INSLAFVFDGVNFGASDFAYSAYSL 534
           INSLAFV DGVNFGASDFAY+AYS+
Sbjct: 491 INSLAFVLDGVNFGASDFAYTAYSM 465

BLAST of Lag0002776 vs. TAIR 10
Match: AT1G51340.2 (MATE efflux family protein )

HSP 1 Score: 475.7 bits (1223), Expect = 5.4e-134
Identity = 280/505 (55.45%), Postives = 353/505 (69.90%), Query Frame = 0

Query: 34  PVNVFFKDARRVFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVGV 93
           P+ +FF D R V KFD +G EI  IALPAALA+ ADP+ASL+DTAF+G IGPVELAAVGV
Sbjct: 15  PLYIFFSDFRSVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGV 74

Query: 94  SIAIFNQASRITIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPEDR 153
           SIA+FNQ SRI IFPLVSITTSFVAEED                C +  ++V+       
Sbjct: 75  SIALFNQVSRIAIFPLVSITTSFVAEEDA---------------CSSQQDTVR------- 134

Query: 154 KLEEDEKLAVKQNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDLE 213
               D K  ++   + +N+ PT   + +     +++K+S S E  T  S+          
Sbjct: 135 ----DHKECIE---IGINN-PTEETIEL---IPEKHKDSLSDEFKTSSSI---------- 194

Query: 214 KDSSTNVTKSTPAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKDN 273
                +++K  PAK     K+ I SAS+ALI G +LGL QA+FL+  AK LL+ MGVK +
Sbjct: 195 ----FSISK-PPAK-----KRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHD 254

Query: 274 SPMLAPAHKYLTLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIF 333
           SPM+ P+ +YL+LRSLGAPAVLLSLA QG+FRGFKDT TPL+  V G   NIILDPI IF
Sbjct: 255 SPMMRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIF 314

Query: 334 VCRWGVKGAAAAHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVVA 393
           V R GV GAA AHV+SQY +  IL W+L+ +V++   S K LQF RF+KNG LLL RV+A
Sbjct: 315 VFRLGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIA 374

Query: 394 VTFCVTLAASLAARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEKT 453
           VTFCVTL+ASLAAR G T MAAFQ CLQVW+ +SLL+DG AVAGQAILASAFA+KDY++ 
Sbjct: 375 VTFCVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRA 434

Query: 454 TATATRVLQMSFILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSLA 513
            ATA+RVLQ+  +LG  LA+++G G+ FGA +F++D  V +LI +G+PF+A TQPIN+LA
Sbjct: 435 AATASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALA 466

Query: 514 FVFDGVNFGASDFAYSAYSLASFGV 539
           FVFDGVNFGASDF Y+A SL    +
Sbjct: 495 FVFDGVNFGASDFGYAAASLVMVAI 466

BLAST of Lag0002776 vs. TAIR 10
Match: AT1G51340.1 (MATE efflux family protein )

HSP 1 Score: 464.2 bits (1193), Expect = 1.6e-130
Identity = 275/494 (55.67%), Postives = 346/494 (70.04%), Query Frame = 0

Query: 45  VFKFDAIGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVGVSIAIFNQASRI 104
           V KFD +G EI  IALPAALA+ ADP+ASL+DTAF+G IGPVELAAVGVSIA+FNQ SRI
Sbjct: 20  VLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSRI 79

Query: 105 TIFPLVSITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPEDRKLEEDEKLAVK 164
            IFPLVSITTSFVAEED                C +  ++V+           D K  ++
Sbjct: 80  AIFPLVSITTSFVAEEDA---------------CSSQQDTVR-----------DHKECIE 139

Query: 165 QNSVNLNHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDLEKDSSTNVTKST 224
              + +N+ PT   + +     +++K+S S E  T  S+               +++K  
Sbjct: 140 ---IGINN-PTEETIEL---IPEKHKDSLSDEFKTSSSI--------------FSISK-P 199

Query: 225 PAKSKKKEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYL 284
           PAK     K+ I SAS+ALI G +LGL QA+FL+  AK LL+ MGVK +SPM+ P+ +YL
Sbjct: 200 PAK-----KRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPMMRPSQRYL 259

Query: 285 TLRSLGAPAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIFVCRWGVKGAAA 344
           +LRSLGAPAVLLSLA QG+FRGFKDT TPL+  V G   NIILDPI IFV R GV GAA 
Sbjct: 260 SLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFRLGVTGAAT 319

Query: 345 AHVLSQYFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVVAVTFCVTLAASL 404
           AHV+SQY +  IL W+L+ +V++   S K LQF RF+KNG LLL RV+AVTFCVTL+ASL
Sbjct: 320 AHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTFCVTLSASL 379

Query: 405 AARLGPTPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEKTTATATRVLQMS 464
           AAR G T MAAFQ CLQVW+ +SLL+DG AVAGQAILASAFA+KDY++  ATA+RVLQ+ 
Sbjct: 380 AAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAATASRVLQLG 439

Query: 465 FILGVGLAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGAS 524
            +LG  LA+++G G+ FGA +F++D  V +LI +G+PF+A TQPIN+LAFVFDGVNFGAS
Sbjct: 440 LVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVFDGVNFGAS 460

Query: 525 DFAYSAYSLASFGV 539
           DF Y+A SL    +
Sbjct: 500 DFGYAAASLVMVAI 460

BLAST of Lag0002776 vs. TAIR 10
Match: AT2G38330.1 (MATE efflux family protein )

HSP 1 Score: 303.1 bits (775), Expect = 4.8e-82
Identity = 192/487 (39.43%), Postives = 272/487 (55.85%), Query Frame = 0

Query: 51  IGREILGIALPAALAVAADPVASLIDTAFVGHIGPVELAAVGVSIAIFNQASRITIFPLV 110
           IG EI+ IALPAALA+AADP+ SL+DTAFVGHIG  ELAAVGVS+++FN  S++   PL+
Sbjct: 76  IGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPLL 135

Query: 111 SITTSFVAEEDTIGKAATKAAKGDKGKCLADDNSVKVHVPEDRKLEEDEKLAVKQNSVNL 170
           ++TTSFVAEE  I      AAK        DDN                           
Sbjct: 136 NVTTSFVAEEQAI------AAK--------DDND-------------------------- 195

Query: 171 NHEPTRSNVTIEQGAGKENKESSSTEKGTKESVPDNGALQDLEKDSSTNVTKSTPAKSKK 230
                                                                    S +
Sbjct: 196 ---------------------------------------------------------SIE 255

Query: 231 KEKKQIASASTALIFGSILGLMQAIFLVFGAKSLLNLMGVKDNSPMLAPAHKYLTLRSLG 290
             KK + S ST+L+  + +G+ +AI L  G+  L+++M +  +SPM  PA ++L LR+ G
Sbjct: 256 TSKKVLPSVSTSLVLAAGVGIAEAIALSLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYG 315

Query: 291 APAVLLSLAMQGIFRGFKDTRTPLYVIVSGYTVNIILDPILIFVCRWGVKGAAAAHVLSQ 350
           AP ++++LA QG FRGFKDT TPLY +V+G  +N +LDPILIFV  +G+ GAAAA V+S+
Sbjct: 316 APPIVVALAAQGAFRGFKDTTTPLYAVVAGNVLNAVLDPILIFVLGFGISGAAAATVISE 375

Query: 351 YFIVLILFWRLVQKVNLMPPSIKDLQFGRFLKNGGLLLARVVAVTFCVTLAASLAARLGP 410
           Y I  IL W+L + V L+ P IK  +  ++LK+GGLL+ R VA+    TLA SLAA+ GP
Sbjct: 376 YLIAFILLWKLNENVVLLSPQIKVGRANQYLKSGGLLIGRTVALLVPFTLATSLAAQNGP 435

Query: 411 TPMAAFQTCLQVWMTSSLLSDGLAVAGQAILASAFAEKDYEKTTATATRVLQMSFILGVG 470
           T MA  Q  L++W+  SLL+D LA+A Q++LA+ +++ +Y++       VLQ+    G G
Sbjct: 436 TQMAGHQIVLEIWLAVSLLTDALAIAAQSLLATTYSQGEYKQAREVLFGVLQVGLATGTG 465

Query: 471 LAIVVGIGMFFGAGIFSRDIHVQYLIHLGIPFIAATQPINSLAFVFDGVNFGASDFAYSA 530
           LA V+ I     + +F+ D  V  +   G  F+A +QP+N+LAFV DG+ +G SDF ++A
Sbjct: 496 LAAVLFITFEPFSSLFTTDSEVLKIALSGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAA 465

Query: 531 YSLASFG 538
           YS+   G
Sbjct: 556 YSMVIVG 465