Lag0002746 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002746
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cytochrome b5 domain-containing protein RLF-like
Location	chr4: 45296640 .. 45299040 (+)
RNA-Seq Expression	Lag0002746
Synteny	Lag0002746

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCAGAACAATTTCTTGGAGTTCTGTTGTTGGAAGAGAGAGGTTGCTTGAAATTTGAGACTGAATAGACTAAGATGGACAGTGACAACGACTTCACATTTTGTCAGGCAAGTATAGTTTCTATGCTATAAAATTAGGTTTTAAGTTTTCAACTCAATGGCATGTTTCCTTCTTTCTATACTTTTACCTTCTACAGTTGGAATAATGTTTGATATCCAACCTAAGTCATAGTACTATTGCTGTTATATGAATATATTAGTAGGTGGGTTTACCTGAAAAAGATGATAAAGAGGCGAAGGATCTTGCATCAGATATAGGTGATATTACCTTAAGGGATGAATTGACGAATGACACTGTTAGTAGGATGCATGCCGTTTGGAATGAGAAATTGTCAAATGATATCACTTCCAGAAAGCAGGCAACAGTGGGTTCCTTGCATTTCAATGTTATTGATATGTCATCTTCAAAACAAGAAGAGGTGTCGAAAGATGCTGGAAACGTTGTGAAGAATTCACGTAAGCCTGCAACTCGAACGAAGGTTCCTTTTGAAAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACCCATCCTGATCTTGCAGGTATTTGTGGAAATGGTATCCAGTTTAACTGCCTTGCATTTTGATATCATGATTTGCTTGAGCCTATCTGGCCACTCTTTTATTCTTCTTGTTAAGACGGTTTTGTATCTTTTAGTTTGGGTTAAAAACACTCATGGTCTTGAACTTTCATGAAAATAACCAATTTTTTTTTTTTAAATTTTAGTTTGTAATAAGTCCATGTACTTTCAAATTTCTAAGAATTTAGCCATTGAACTTTAGTATGTAATAATTTAGTCCTTATACTTTCAAATTTATAACAATTTAATCCAAGCCGTGAAAAATTGCATCAAAATTAAGTGTCAATTTTTATGATTTAACGAGTTAGTCTTTGTAGTTTATAAACACATTTGGTTTCTAATCAATTTATCGATCTACATATGTTAGAAATTCCATTAAATCTTAACACCATTTTTTGATGATAGGGACTAAATTGTTACAAAATTGAAAGTGTACGGATGAAATTGTTTGAAACTAACATTTAGGGACTAAAAGTCTTTTTTAACCTTAATTTTATGACCTGGCACCTTGCCTTATGTTTATGTTTGTTTGGGCTATTAAAGAGATATAAATTGTTTGCAGCTAGATGATGCTTGGCATGTTCAGGATTCAGGAAAGATGCTAAAAGATCTATTTTATCTAAATTATGAACTTCATAGAGGACCCACTTGCCATTTTCGTTGTGTCTATAAAAGTACAGTATTAATATGATAGTTATTGTTTGTGTTGCGAAATAGGATCATTTTATTGTTTTCATGATTACTGGTGGTTTAATGTTATCAATTTCCCCATTGAGAAATAATGCAATAGATTTTCAACATTTGTTTCATAATTGTGGATTAGGGTTGAAAGGTCAGTCAAATAAAAGGCTTATCCCTATGAGTGAAGTTAAACAACATCAGATGGAGGGCTCCATGTGGACTGTTCTGAAAGGTCGTGTGTACAATATATCTCCTTATATGAAGTTTCACCCGGGAGGTATGGGCATCATGCAAAAATTTCTATGTTTAACTTTTCTTTTAGTGAATTTATTATCCATTCCATCCTGCTATAATGAGCTTTTCTCACTCAGAGGACTCTAGAGTTGGTAAAGTAGCATGATTACTTTGTAATGTATGTTATGGTGTCTCGTGTTCAAGAATTGCCAGAGTCTATAGCTTCTTCTAATATTTTATATTTAAGAGAGCATTTAAAGTTTAACTTATAGGAAATTGCCTACAGTATCATGTTATTGAATTCTTTGAAGGATGAATGATTATTCTCCTTTATTCGTGACCTAGGTAGGAAGCTTAGCTCATGAATACTGGCTTCTAGTTCTACTTATATATTGCTGGTTGATTTTCTGATGTACTTTCTAGTTATTATTTGGGATGATTCTTGTATGTTTTAAATGATGGGTGAGTTTTATTAGAGTGCATCATAAACATTTTCTTGGGGGTAGACTTGGACTACTTGACATTTTGACAAACTTCTTTTATCAGGTGCTGATATACTCATGAAGGCAGTGGGAAAAGATTGTACATCTTTATTCAGTATCCTTGAATTTTGTTTATTTATTTTTTGGTTAAAAAGTATCACTTATCCCTCTATGGTATTACTGTTGCCACGTACACCTAGTTATTTTTTCGTGCAACTTCCAACGGTTCATTTAATGAATTCTGAATCTGTTGAAAAGTTATCCTTAACAACCTTTAGATAAATACCACGCATGGGTCAACGCTGAATTTTTGCTGGAGAAGTGTCTAGTTGGCACTTTAGATGATAGTCATTGA

mRNA sequence

ATGCAGCAGAACAATTTCTTGGAGTTCTGTTGTTGGAAGAGAGAGGTGGGTTTACCTGAAAAAGATGATAAAGAGGCGAAGGATCTTGCATCAGATATAGGTGATATTACCTTAAGGGATGAATTGACGAATGACACTGTTAGTAGGATGCATGCCGTTTGGAATGAGAAATTGTCAAATGATATCACTTCCAGAAAGCAGGCAACAGTGGGTTCCTTGCATTTCAATGTTATTGATATGTCATCTTCAAAACAAGAAGAGGTGTCGAAAGATGCTGGAAACGTTGTGAAGAATTCACGTAAGCCTGCAACTCGAACGAAGGTTCCTTTTGAAAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACCCATCCTGATCTTGCAGGGTTGAAAGGTCAGTCAAATAAAAGGCTTATCCCTATGAGTGAAGTTAAACAACATCAGATGGAGGGCTCCATGTGGACTGTTCTGAAAGGTCGTGTGTACAATATATCTCCTTATATGAAGTTTCACCCGGGAGGTGCTGATATACTCATGAAGGCAGTGGGAAAAGATTGTACATCTTTATTCAATAAATACCACGCATGGGTCAACGCTGAATTTTTGCTGGAGAAGTGTCTAGTTGGCACTTTAGATGATAGTCATTGA

Coding sequence (CDS)

ATGCAGCAGAACAATTTCTTGGAGTTCTGTTGTTGGAAGAGAGAGGTGGGTTTACCTGAAAAAGATGATAAAGAGGCGAAGGATCTTGCATCAGATATAGGTGATATTACCTTAAGGGATGAATTGACGAATGACACTGTTAGTAGGATGCATGCCGTTTGGAATGAGAAATTGTCAAATGATATCACTTCCAGAAAGCAGGCAACAGTGGGTTCCTTGCATTTCAATGTTATTGATATGTCATCTTCAAAACAAGAAGAGGTGTCGAAAGATGCTGGAAACGTTGTGAAGAATTCACGTAAGCCTGCAACTCGAACGAAGGTTCCTTTTGAAAAGGGGTATAGCCAAATGGATTGGCTTAAGCTTACTCGAACCCATCCTGATCTTGCAGGGTTGAAAGGTCAGTCAAATAAAAGGCTTATCCCTATGAGTGAAGTTAAACAACATCAGATGGAGGGCTCCATGTGGACTGTTCTGAAAGGTCGTGTGTACAATATATCTCCTTATATGAAGTTTCACCCGGGAGGTGCTGATATACTCATGAAGGCAGTGGGAAAAGATTGTACATCTTTATTCAATAAATACCACGCATGGGTCAACGCTGAATTTTTGCTGGAGAAGTGTCTAGTTGGCACTTTAGATGATAGTCATTGA

Protein sequence

MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSNDITSRKQATVGSLHFNVIDMSSSKQEEVSKDAGNVVKNSRKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH

Homology

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BLAST of Lag0002746 vs. NCBI nr
Match: XP_038899914.1 (cytochrome b5 domain-containing protein RLF [Benincasa hispida] >XP_038899915.1 cytochrome b5 domain-containing protein RLF [Benincasa hispida])

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-98
Identity = 187/230 (81.30%), Postives = 198/230 (86.09%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M   +   FC    +VGLPE++++EAKDLASDIGDITLRDEL N TVSR H +WN+KLSN
Sbjct: 1   MDSEDDFTFC----QVGLPERNNREAKDLASDIGDITLRDELANGTVSRTHVIWNDKLSN 60

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           +ITSRKQATVGSL FNVIDMSSSKQ      EEV KDAG VVKNS       RKPA RTK
Sbjct: 61  NITSRKQATVGSLDFNVIDMSSSKQSSTLSTEEVPKDAGKVVKNSGKQGIAVRKPAARTK 120

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+H+ EGSMWTVLKGRVYNIS
Sbjct: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHRTEGSMWTVLKGRVYNIS 180

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH
Sbjct: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 226

[Close]

BLAST of Lag0002746 vs. NCBI nr
Match: XP_004135940.2 (cytochrome b5 domain-containing protein RLF isoform X1 [Cucumis sativus] >KGN45109.1 hypothetical protein Csa_016547 [Cucumis sativus])

HSP 1 Score: 365.9 bits (938), Expect = 2.4e-97
Identity = 187/249 (75.10%), Postives = 198/249 (79.52%), Query Frame = 0


Query: 3   QNNFLEFCCWKR---------------------EVGLPEKDDKEAKDLASDIGDITLRDE 62
           QNNF++F CW+                      +VGLPE++DKEAKDLASDIG ITLRDE
Sbjct: 66  QNNFVKFHCWREVWLQFESKYTEMDSEDDFTFCQVGLPERNDKEAKDLASDIGHITLRDE 125

Query: 63  LTNDTVSRMHAVWNEKLSNDITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNV 122
           L N T SR   VWN+KLSNDITSRKQATVGSL FNV+DMS SKQ      EEV KDAG V
Sbjct: 126 LANGTTSRTRVVWNDKLSNDITSRKQATVGSLDFNVLDMSYSKQSSTLSTEEVPKDAGKV 185

Query: 123 VKNS-------RKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQ 182
           VKNS       RKPA R KVPFEKGYSQMDWLKLT+THPDLAGLKGQSNKRLIPMSEVK+
Sbjct: 186 VKNSGKQGIAVRKPAARKKVPFEKGYSQMDWLKLTQTHPDLAGLKGQSNKRLIPMSEVKE 245

Query: 183 HQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKC 218
           H+ EGSMWTVLKG VYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKC
Sbjct: 246 HRTEGSMWTVLKGHVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKC 305

[Close]

BLAST of Lag0002746 vs. NCBI nr
Match: XP_022960400.1 (cytochrome b5 domain-containing protein RLF-like [Cucurbita moschata] >XP_022960401.1 cytochrome b5 domain-containing protein RLF-like [Cucurbita moschata])

HSP 1 Score: 364.0 bits (933), Expect = 9.0e-97
Identity = 185/230 (80.43%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M  +N   FC    +VGLPEKDDKEAKDLA DIGDITLRDELTN  VSR + +W++ LSN
Sbjct: 4   MDSDNDFTFC----QVGLPEKDDKEAKDLAYDIGDITLRDELTNGAVSRTNVIWDDNLSN 63

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           DITSRKQATVGSLHFNV DMSSSKQ      E+V KDA  V K S       R+PATRTK
Sbjct: 64  DITSRKQATVGSLHFNVTDMSSSKQSSTQSTEKVPKDAEKVAKVSGKQGTAGRRPATRTK 123

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYNIS
Sbjct: 124 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNIS 183

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLD+SH
Sbjct: 184 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDNSH 229

[Close]

BLAST of Lag0002746 vs. NCBI nr
Match: XP_023515005.1 (cytochrome b5 domain-containing protein RLF-like [Cucurbita pepo subsp. pepo] >XP_023515006.1 cytochrome b5 domain-containing protein RLF-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.1 bits (928), Expect = 3.4e-96
Identity = 183/230 (79.57%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M  +N   FC    +VGLPEKDDKEAKDLA DIGDITLRDELTN  VSR + +W++ LSN
Sbjct: 4   MDSDNDFTFC----QVGLPEKDDKEAKDLAYDIGDITLRDELTNGAVSRTNVIWDDNLSN 63

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           DITSRKQATVGSLHFNV DMSSSKQ      E+V KDA  V K S       R+PATRTK
Sbjct: 64  DITSRKQATVGSLHFNVTDMSSSKQSSTQSTEKVPKDAEKVAKVSGKQGTAGRRPATRTK 123

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFE+GYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYN+S
Sbjct: 124 VPFERGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNLS 183

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLD+SH
Sbjct: 184 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDNSH 229

[Close]

BLAST of Lag0002746 vs. NCBI nr
Match: XP_008461332.2 (PREDICTED: disease resistance protein RGA2 [Cucumis melo])

HSP 1 Score: 361.7 bits (927), Expect = 4.5e-96
Identity = 185/230 (80.43%), Postives = 192/230 (83.48%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M   +   FC    +VGLPE++DKEAKDLASDIG ITLRDEL N T SR   VWN+KLSN
Sbjct: 1   MDSEDDFTFC----QVGLPERNDKEAKDLASDIGHITLRDELANGTASRTRIVWNDKLSN 60

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           DI SRKQATVGSL FNVIDMSSSKQ      EEV KD G VVKNS       RKPA RTK
Sbjct: 61  DIPSRKQATVGSLDFNVIDMSSSKQSSTLSTEEVPKDTGKVVKNSGKQGIAVRKPAARTK 120

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+H+ EGSMWTVLKGRVYNIS
Sbjct: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHRTEGSMWTVLKGRVYNIS 180

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVN EFLLEKCLVGTLDDSH
Sbjct: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNDEFLLEKCLVGTLDDSH 226

[Close]

BLAST of Lag0002746 vs. ExPASy Swiss-Prot
Match: Q9LXD1 (Cytochrome b5 domain-containing protein RLF OS=Arabidopsis thaliana OX=3702 GN=RLF PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 2.9e-66
Identity = 129/195 (66.15%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 23  DKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSNDITSRKQATVGSLHFNVIDMSS 82
           D E KDLASD+G ITL+D L  D       +W +K        K+ T+GSL F V D SS
Sbjct: 18  DSEVKDLASDVGSITLKDGL--DQQKSNGLIWKDK----SLPPKEETIGSLSFTVTDSSS 77

Query: 83  SKQEEVSKDAGNVVKN-SRKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLI 142
           SK++  S ++    K  +RKP TRTKVPFEKGYSQMDWLKLTRTHPDLAGLKG+SNKRLI
Sbjct: 78  SKKQ--SNESSETFKTPARKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLI 137

Query: 143 PMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNA 202
           PM EVK+H+   SMWTVLKGRVYNISPYM FHPGG D+LMKAVG+D T LFNKYHAWVN 
Sbjct: 138 PMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNV 197

Query: 203 EFLLEKCLVGTLDDS 217
           + LLEKCLVG LDD+
Sbjct: 198 DILLEKCLVGVLDDT 204

[Close]

BLAST of Lag0002746 vs. ExPASy Swiss-Prot
Match: Q32LH7 (Cytochrome b5 reductase 4 OS=Bos taurus OX=9913 GN=CYB5R4 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.0e-27
Identity = 55/111 (49.55%), Postives = 79/111 (71.17%), Query Frame = 0


Query: 105 RTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMS--EVKQHQMEGSMWTVLKGR 164
           R+KVP ++G S MDW++LT++  DL GLKG    RLI ++  E+K+H  +   W  ++G 
Sbjct: 23  RSKVPLKQGRSLMDWIRLTKSGKDLTGLKG----RLIEVTEEELKKHNKKDDCWICIRGF 82

Query: 165 VYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTL 214
           VYN+SPYM++HPGG D LM+A G D T LF++ H WVN E +L++CLVG +
Sbjct: 83  VYNVSPYMEYHPGGEDELMRAAGSDGTDLFDQVHRWVNYESMLKECLVGRM 129

[Close]

BLAST of Lag0002746 vs. ExPASy Swiss-Prot
Match: Q7L1T6 (Cytochrome b5 reductase 4 OS=Homo sapiens OX=9606 GN=CYB5R4 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.0e-27
Identity = 55/111 (49.55%), Postives = 79/111 (71.17%), Query Frame = 0


Query: 105 RTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMS--EVKQHQMEGSMWTVLKGR 164
           R+KVP ++G S MDW++LT++  DL GLKG    RLI ++  E+K+H  +   W  ++G 
Sbjct: 23  RSKVPLKQGRSLMDWIRLTKSGKDLTGLKG----RLIEVTEEELKKHNKKDDCWICIRGF 82

Query: 165 VYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTL 214
           VYN+SPYM++HPGG D LM+A G D T LF++ H WVN E +L++CLVG +
Sbjct: 83  VYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVGRM 129

[Close]

BLAST of Lag0002746 vs. ExPASy Swiss-Prot
Match: Q28CZ9 (Cytochrome b5 reductase 4 OS=Xenopus tropicalis OX=8364 GN=cyb5r4 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.0e-27
Identity = 55/115 (47.83%), Postives = 79/115 (68.70%), Query Frame = 0


Query: 105 RTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMS--EVKQHQMEGSMWTVLKGR 164
           R+KVP + G S MDW++LT++  DL GLKG    RLI ++  E+ QH  +   W  ++G 
Sbjct: 23  RSKVPLKPGRSLMDWIRLTKSGKDLTGLKG----RLIDVTEEELAQHNKKEDCWICIRGM 82

Query: 165 VYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           VYNI+PYM++HPGG + LMKA G+D T LF++ H WVN E +L++CL+G +   H
Sbjct: 83  VYNITPYMEYHPGGEEELMKAAGRDGTDLFDQVHRWVNYESMLKECLIGRMAIKH 133

[Close]

BLAST of Lag0002746 vs. ExPASy Swiss-Prot
Match: Q3TDX8 (Cytochrome b5 reductase 4 OS=Mus musculus OX=10090 GN=Cyb5r4 PE=2 SV=3)

HSP 1 Score: 121.7 bits (304), Expect = 1.0e-26
Identity = 53/109 (48.62%), Postives = 76/109 (69.72%), Query Frame = 0


Query: 105 RTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVY 164
           R+KVP ++G S MDW++LT++  DL GLKG   +  +   E+K+H  +   W  ++G VY
Sbjct: 23  RSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIE--VTEEELKKHNKKEDCWICIRGFVY 82

Query: 165 NISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTL 214
           N+SPYM++HPGG D LM+A G D T LFN+ H WVN E +L++CLVG +
Sbjct: 83  NVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRM 129

[Close]

BLAST of Lag0002746 vs. ExPASy TrEMBL
Match: A0A0A0K899 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G426500 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.1e-97
Identity = 187/249 (75.10%), Postives = 198/249 (79.52%), Query Frame = 0


Query: 3   QNNFLEFCCWKR---------------------EVGLPEKDDKEAKDLASDIGDITLRDE 62
           QNNF++F CW+                      +VGLPE++DKEAKDLASDIG ITLRDE
Sbjct: 66  QNNFVKFHCWREVWLQFESKYTEMDSEDDFTFCQVGLPERNDKEAKDLASDIGHITLRDE 125

Query: 63  LTNDTVSRMHAVWNEKLSNDITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNV 122
           L N T SR   VWN+KLSNDITSRKQATVGSL FNV+DMS SKQ      EEV KDAG V
Sbjct: 126 LANGTTSRTRVVWNDKLSNDITSRKQATVGSLDFNVLDMSYSKQSSTLSTEEVPKDAGKV 185

Query: 123 VKNS-------RKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQ 182
           VKNS       RKPA R KVPFEKGYSQMDWLKLT+THPDLAGLKGQSNKRLIPMSEVK+
Sbjct: 186 VKNSGKQGIAVRKPAARKKVPFEKGYSQMDWLKLTQTHPDLAGLKGQSNKRLIPMSEVKE 245

Query: 183 HQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKC 218
           H+ EGSMWTVLKG VYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKC
Sbjct: 246 HRTEGSMWTVLKGHVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKC 305

[Close]

BLAST of Lag0002746 vs. ExPASy TrEMBL
Match: A0A6J1H7H2 (cytochrome b5 domain-containing protein RLF-like OS=Cucurbita moschata OX=3662 GN=LOC111461137 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 4.3e-97
Identity = 185/230 (80.43%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M  +N   FC    +VGLPEKDDKEAKDLA DIGDITLRDELTN  VSR + +W++ LSN
Sbjct: 4   MDSDNDFTFC----QVGLPEKDDKEAKDLAYDIGDITLRDELTNGAVSRTNVIWDDNLSN 63

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           DITSRKQATVGSLHFNV DMSSSKQ      E+V KDA  V K S       R+PATRTK
Sbjct: 64  DITSRKQATVGSLHFNVTDMSSSKQSSTQSTEKVPKDAEKVAKVSGKQGTAGRRPATRTK 123

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYNIS
Sbjct: 124 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNIS 183

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLD+SH
Sbjct: 184 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDNSH 229

[Close]

BLAST of Lag0002746 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.2e-96
Identity = 185/230 (80.43%), Postives = 192/230 (83.48%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M   +   FC    +VGLPE++DKEAKDLASDIG ITLRDEL N T SR   VWN+KLSN
Sbjct: 1   MDSEDDFTFC----QVGLPERNDKEAKDLASDIGHITLRDELANGTASRTRIVWNDKLSN 60

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           DI SRKQATVGSL FNVIDMSSSKQ      EEV KD G VVKNS       RKPA RTK
Sbjct: 61  DIPSRKQATVGSLDFNVIDMSSSKQSSTLSTEEVPKDTGKVVKNSGKQGIAVRKPAARTK 120

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+H+ EGSMWTVLKGRVYNIS
Sbjct: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHRTEGSMWTVLKGRVYNIS 180

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVN EFLLEKCLVGTLDDSH
Sbjct: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNDEFLLEKCLVGTLDDSH 226

[Close]

BLAST of Lag0002746 vs. ExPASy TrEMBL
Match: A0A6J1KSP4 (cytochrome b5 domain-containing protein RLF OS=Cucurbita maxima OX=3661 GN=LOC111497877 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.7e-96
Identity = 182/230 (79.13%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLPEKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSN 60
           M  +N   FC    +VGLPEKDDKEAKDLA DIGDITLRDELTN  VSR + +W++ LSN
Sbjct: 4   MDSDNDFTFC----QVGLPEKDDKEAKDLAYDIGDITLRDELTNGAVSRTNVIWDDNLSN 63

Query: 61  DITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPATRTK 120
           DITSRKQATVGSLHFNV DMSS+KQ      E+V KDA  V K S       R+PATRTK
Sbjct: 64  DITSRKQATVGSLHFNVTDMSSAKQSSTQSTEKVPKDAEKVAKVSGKQGTAGRRPATRTK 123

Query: 121 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRVYNIS 180
           VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVK+HQ EGSMWTVLKGRVYN+S
Sbjct: 124 VPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKEHQTEGSMWTVLKGRVYNLS 183

Query: 181 PYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           PYMKFHPGGADILMKAVG+DCTSLFNKYHAWVNAEFLLEKCLVGTLD+SH
Sbjct: 184 PYMKFHPGGADILMKAVGRDCTSLFNKYHAWVNAEFLLEKCLVGTLDNSH 229

[Close]

BLAST of Lag0002746 vs. ExPASy TrEMBL
Match: A0A6J1D8C4 (cytochrome b5 domain-containing protein RLF OS=Momordica charantia OX=3673 GN=LOC111017852 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.0e-93
Identity = 181/234 (77.35%), Postives = 192/234 (82.05%), Query Frame = 0


Query: 1   MQQNNFLEFCCWKREVGLP----EKDDKEAKDLASDIGDITLRDELTNDTVSRMHAVWNE 60
           M  +N   FC    +VGLP    EKDD  AKDLA +IGDITLR+EL N T+S  H  WN+
Sbjct: 1   MDSDNDFTFC----QVGLPSEGDEKDDIRAKDLALEIGDITLREELANGTISSPHVGWND 60

Query: 61  KLSNDITSRKQATVGSLHFNVIDMSSSKQ------EEVSKDAGNVVKNS-------RKPA 120
           KLS DITS KQA VGSLHFNVIDMSS+KQ      EE++KDAG  V+ S       RKPA
Sbjct: 61  KLSTDITSNKQAMVGSLHFNVIDMSSAKQSSALSTEEMAKDAGKAVRTSGKQGTAARKPA 120

Query: 121 TRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQMEGSMWTVLKGRV 180
           TRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQ EGSMWTVLKGRV
Sbjct: 121 TRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLIPMSEVKQHQTEGSMWTVLKGRV 180

Query: 181 YNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGTLDDSH 218
           YNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVN+EFLLEKCLVGTLDDSH
Sbjct: 181 YNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNSEFLLEKCLVGTLDDSH 230

[Close]

BLAST of Lag0002746 vs. TAIR 10
Match: AT5G09680.1 (reduced lateral root formation )

HSP 1 Score: 253.1 bits (645), Expect = 2.1e-67
Identity = 129/195 (66.15%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 23  DKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSNDITSRKQATVGSLHFNVIDMSS 82
           D E KDLASD+G ITL+D L  D       +W +K        K+ T+GSL F V D SS
Sbjct: 18  DSEVKDLASDVGSITLKDGL--DQQKSNGLIWKDK----SLPPKEETIGSLSFTVTDSSS 77

Query: 83  SKQEEVSKDAGNVVKN-SRKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLI 142
           SK++  S ++    K  +RKP TRTKVPFEKGYSQMDWLKLTRTHPDLAGLKG+SNKRLI
Sbjct: 78  SKKQ--SNESSETFKTPARKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLI 137

Query: 143 PMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNA 202
           PM EVK+H+   SMWTVLKGRVYNISPYM FHPGG D+LMKAVG+D T LFNKYHAWVN 
Sbjct: 138 PMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNV 197

Query: 203 EFLLEKCLVGTLDDS 217
           + LLEKCLVG LDD+
Sbjct: 198 DILLEKCLVGVLDDT 204

[Close]

BLAST of Lag0002746 vs. TAIR 10
Match: AT5G09680.2 (reduced lateral root formation )

HSP 1 Score: 253.1 bits (645), Expect = 2.1e-67
Identity = 129/195 (66.15%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 23  DKEAKDLASDIGDITLRDELTNDTVSRMHAVWNEKLSNDITSRKQATVGSLHFNVIDMSS 82
           D E KDLASD+G ITL+D L  D       +W +K        K+ T+GSL F V D SS
Sbjct: 18  DSEVKDLASDVGSITLKDGL--DQQKSNGLIWKDK----SLPPKEETIGSLSFTVTDSSS 77

Query: 83  SKQEEVSKDAGNVVKN-SRKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLI 142
           SK++  S ++    K  +RKP TRTKVPFEKGYSQMDWLKLTRTHPDLAGLKG+SNKRLI
Sbjct: 78  SKKQ--SNESSETFKTPARKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLI 137

Query: 143 PMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAWVNA 202
           PM EVK+H+   SMWTVLKGRVYNISPYM FHPGG D+LMKAVG+D T LFNKYHAWVN 
Sbjct: 138 PMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNV 197

Query: 203 EFLLEKCLVGTLDDS 217
           + LLEKCLVG LDD+
Sbjct: 198 DILLEKCLVGVLDDT 204

[Close]

BLAST of Lag0002746 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-12
Identity = 31/78 (39.74%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 139 RLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAW 198
           +L  M E   H  +   W V+ G+VY++S YM  HPGG D+L+   GKD T  F      
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 199 VNAEFLLEKCLVGTLDDS 217
            +A  L+EK  +G LD+S
Sbjct: 66  KDARELMEKYFIGELDES 83

[Close]

BLAST of Lag0002746 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 68.6 bits (166), Expect = 7.3e-12
Identity = 25/81 (30.86%), Postives = 48/81 (59.26%), Query Frame = 0


Query: 136 SNKRLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKY 195
           S+++++   EV +H      W ++ G+VY+++P+M  HPGG ++L+ + GKD T+ F   
Sbjct: 3   SDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 196 HAWVNAEFLLEKCLVGTLDDS 217
                A  +++K  +G +D S
Sbjct: 63  GHSDTARDMMDKYFIGEIDSS 83

[Close]

BLAST of Lag0002746 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 26/76 (34.21%), Postives = 45/76 (59.21%), Query Frame = 0


Query: 139 RLIPMSEVKQHQMEGSMWTVLKGRVYNISPYMKFHPGGADILMKAVGKDCTSLFNKYHAW 198
           ++  +SEV +H      W V+ G+VYN++ +++ HPGG D+L+ + GKD T  F      
Sbjct: 6   KIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 65

Query: 199 VNAEFLLEKCLVGTLD 215
            +A  ++E+  VG +D
Sbjct: 66  ESAREMMEQYYVGEID 81

The following BLAST results are available for this feature:

BLAST of Lag0002746 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899914.1	1.3e-98	81.30	cytochrome b5 domain-containing protein RLF [Benincasa hispida] >XP_038899915.1 ...	[more]
XP_004135940.2	2.4e-97	75.10	cytochrome b5 domain-containing protein RLF isoform X1 [Cucumis sativus] >KGN451...	[more]
XP_022960400.1	9.0e-97	80.43	cytochrome b5 domain-containing protein RLF-like [Cucurbita moschata] >XP_022960...	[more]
XP_023515005.1	3.4e-96	79.57	cytochrome b5 domain-containing protein RLF-like [Cucurbita pepo subsp. pepo] >X...	[more]
XP_008461332.2	4.5e-96	80.43	PREDICTED: disease resistance protein RGA2 [Cucumis melo]	[more]

BLAST of Lag0002746 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXD1	2.9e-66	66.15	Cytochrome b5 domain-containing protein RLF OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q32LH7	6.0e-27	49.55	Cytochrome b5 reductase 4 OS=Bos taurus OX=9913 GN=CYB5R4 PE=2 SV=1	[more]
Q7L1T6	6.0e-27	49.55	Cytochrome b5 reductase 4 OS=Homo sapiens OX=9606 GN=CYB5R4 PE=1 SV=1	[more]
Q28CZ9	6.0e-27	47.83	Cytochrome b5 reductase 4 OS=Xenopus tropicalis OX=8364 GN=cyb5r4 PE=2 SV=1	[more]
Q3TDX8	1.0e-26	48.62	Cytochrome b5 reductase 4 OS=Mus musculus OX=10090 GN=Cyb5r4 PE=2 SV=3	[more]

BLAST of Lag0002746 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K899	1.1e-97	75.10	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1H7H2	4.3e-97	80.43	cytochrome b5 domain-containing protein RLF-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A1S3CEH4	2.2e-96	80.43	disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=...	[more]
A0A6J1KSP4	3.7e-96	79.13	cytochrome b5 domain-containing protein RLF OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1D8C4	1.0e-93	77.35	cytochrome b5 domain-containing protein RLF OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of Lag0002746 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09680.1	2.1e-67	66.15	reduced lateral root formation	[more]
AT5G09680.2	2.1e-67	66.15	reduced lateral root formation	[more]
AT1G26340.1	1.5e-12	39.74	cytochrome B5 isoform A	[more]
AT5G53560.1	7.3e-12	30.86	cytochrome B5 isoform E	[more]
AT2G32720.1	2.1e-11	34.21	cytochrome B5 isoform B	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 201..213 score: 34.97 coord: 163..173 score: 33.2 coord: 173..187 score: 49.29
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 141..214 e-value: 3.8E-22 score: 89.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 143..213 e-value: 4.8E-22 score: 77.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 138..214 score: 20.859018
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 135..216 e-value: 8.5E-29 score: 101.4
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 138..216
None	No IPR available	PANTHER	PTHR43112	FERREDOXIN	coord: 15..208
None	No IPR available	PANTHER	PTHR43112:SF5	CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN RLF	coord: 15..208
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 169..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002746.1	Lag0002746.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
cellular_component	GO:0005829	cytosol
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding