Lag0002696 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0002696
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptiontolB protein-related
Locationchr4: 44912557 .. 44914497 (+)
RNA-Seq ExpressionLag0002696
SyntenyLag0002696
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAACATCCCCACCGGCACCGTCATCTTCACCACCGTCGACCGCCTCCAATACGGCTTCGACACCTTCTCTGTTCCCATCAATTCCCCCACCGTCGAACATCGCCTCACCGACGGAATCTCCGTCAATTTCAACGCCCAATTCCTCCAAAACGACCAATCCATTGTCTTCATCTCTGAACGATCCGGCTCCCCTAGAATTTACCTCTCAAATTCCCCCGATTCCGCCCCTAAATTGCTCCCCTCCGCTCCCGGAAGCTGCTTTCACGACCGACCCATCGTCAGAAATGGTCGCCTCTATTTCATCTCCGCTCATGAAAACCCCCACAAGCCCTTAACGAGCTGGTCCGCTCTGTATTCGACCGGGTTGGACGGCTCCGATCCGGTGACCCGGCTCACTCCTCCTGGGTCCGTCGATTTCAGTCCAGCGGTTTCTGAGAGTGGGAAGTTCTTGGCGGTTGCCTCTTATGGATCTCGTTCTTGGGGCGGCGAATTCAACGAGCTCCACACGGAGATTGTTGTGTTCAAAGCTTCCGACCCGACTGGTCGGGTCGTTGTTTCGTGTCGCGGCGGATGGCCGTCGTGGTCTGGCGACTCCGCTGTTTACTTCCACCGGGTGGCCGACGATGGGTGGTGGAGCATTTTCAGGGTGGAGATTCCCGAAAATCTCGATTCCTCTGTTTCCCCTGTTCCGATCCGGGTCACTCCGGCGGGTCTCCATTGCTTCACTCCAGCGGCCATGAACGACTGCAATCGAGTCGTCGTCGCCACCCGAAGACCCGACAGTAAATTCCGACACATCGAAATTTTCGATTCCGGGTCGGACGAATTCGTCCCGATTACCCAGAAATTGAATCCGAATTTCCATCACTACAACCCGTTCGTCTCGCCGGATTCCAATTACGTCGGGTACCACCGGTTCCGCGGCGAGTCAAGTCAGGGCGAGTTAACGATTCCACATCTCAACTCGGTAATTTCCCCGATTAATGAACTCCGTATGATCCGATTAAACGGTTCGTTCCCAACGCCGTCACCTGACGGCGATTTCATCGCGTTCAATCCCGATTTTGTGGGGTTAAAAATCGTCAAATCAGACGGCTCAAAATGTTGGACCGTACTGAAAGAACGTACTGCGTTTTACAATTCATGGAGCCCCACGGAAAAAAACGTGATTTACACTTCGCTGGGGCCCATTTTTGGGCCACCGAGAGCGACGGTCCAGATCGCTCGGATCACAATCAACTCCGACGATCTCAACGGTGGTGATAACGAGGAAGTCGCCAGTGAAGTGAAGATTCTGACGAAAGACGACAGCGGAAATAACGCATTTCCAGCGTGTTCGCCGGACGGGAAGTTTCTAGTTTTCCGGTCCGGCAGGTCCGGGCATAAGAATCTTTACATCGTCGACGCCGTGAACGGCGAGTTCGACGGCGGCATACGGCGGTTGACCGACGGACCGTGGATCGACACAATGCCGAGCTGGTCGCCGACGGGGGATCTGATCGCGTTTTCTTCGAATATGCATAATCCGAAAAATAGTGAAGTGTTTAGTATCTACGTCATCAGACCGGACGGTTCGGGTTTGCGAAGGGTACACGTGGCTGGACCAGATGGGTGTGGTGACGTGGACAAGGAGAGGATTAACCACGTGTGCTTTAGTAGGGATGGGCAGTGGCTCTTATTTACGTCGAATTTGGGTGGGGTGACAGCGGAGCCAGTGTCGCTGCCCAATCAGTTTCAGCCATATGGAGATTTGTTTGTGGTGAGGTTGGATGGGACCGGGCTAAGGAGGCTGACGTGGAATGGATATGAAAATGGAACTCCCACGTGGTATTATGGAAGTGAGCTGGCACTTTCTGGGTTGAGTTTGAAAGATAATGTGGTTGGTGAGAAGTTGGCGGGAAAGTTTGTTGAACCGCTTTGGATAACGTTCCATTAA

mRNA sequence

ATGGACAACATCCCCACCGGCACCGTCATCTTCACCACCGTCGACCGCCTCCAATACGGCTTCGACACCTTCTCTGTTCCCATCAATTCCCCCACCGTCGAACATCGCCTCACCGACGGAATCTCCGTCAATTTCAACGCCCAATTCCTCCAAAACGACCAATCCATTGTCTTCATCTCTGAACGATCCGGCTCCCCTAGAATTTACCTCTCAAATTCCCCCGATTCCGCCCCTAAATTGCTCCCCTCCGCTCCCGGAAGCTGCTTTCACGACCGACCCATCGTCAGAAATGGTCGCCTCTATTTCATCTCCGCTCATGAAAACCCCCACAAGCCCTTAACGAGCTGGTCCGCTCTGTATTCGACCGGGTTGGACGGCTCCGATCCGGTGACCCGGCTCACTCCTCCTGGGTCCGTCGATTTCAGTCCAGCGGTTTCTGAGAGTGGGAAGTTCTTGGCGGTTGCCTCTTATGGATCTCGTTCTTGGGGCGGCGAATTCAACGAGCTCCACACGGAGATTGTTGTGTTCAAAGCTTCCGACCCGACTGGTCGGGTCGTTGTTTCGTGTCGCGGCGGATGGCCGTCGTGGTCTGGCGACTCCGCTGTTTACTTCCACCGGGTGGCCGACGATGGGTGGTGGAGCATTTTCAGGGTGGAGATTCCCGAAAATCTCGATTCCTCTGTTTCCCCTGTTCCGATCCGGGTCACTCCGGCGGGTCTCCATTGCTTCACTCCAGCGGCCATGAACGACTGCAATCGAGTCGTCGTCGCCACCCGAAGACCCGACAGTAAATTCCGACACATCGAAATTTTCGATTCCGGGTCGGACGAATTCGTCCCGATTACCCAGAAATTGAATCCGAATTTCCATCACTACAACCCGTTCGTCTCGCCGGATTCCAATTACGTCGGGTACCACCGGTTCCGCGGCGAGTCAAGTCAGGGCGAGTTAACGATTCCACATCTCAACTCGGTAATTTCCCCGATTAATGAACTCCGTATGATCCGATTAAACGGTTCGTTCCCAACGCCGTCACCTGACGGCGATTTCATCGCGTTCAATCCCGATTTTGTGGGGTTAAAAATCGTCAAATCAGACGGCTCAAAATGTTGGACCGTACTGAAAGAACGTACTGCGTTTTACAATTCATGGAGCCCCACGGAAAAAAACGTGATTTACACTTCGCTGGGGCCCATTTTTGGGCCACCGAGAGCGACGGTCCAGATCGCTCGGATCACAATCAACTCCGACGATCTCAACGGTGGTGATAACGAGGAAGTCGCCAGTGAAGTGAAGATTCTGACGAAAGACGACAGCGGAAATAACGCATTTCCAGCGTGTTCGCCGGACGGGAAGTTTCTAGTTTTCCGGTCCGGCAGGTCCGGGCATAAGAATCTTTACATCGTCGACGCCGTGAACGGCGAGTTCGACGGCGGCATACGGCGGTTGACCGACGGACCGTGGATCGACACAATGCCGAGCTGGTCGCCGACGGGGGATCTGATCGCGTTTTCTTCGAATATGCATAATCCGAAAAATAGTGAAGTGTTTAGTATCTACGTCATCAGACCGGACGGTTCGGGTTTGCGAAGGGTACACGTGGCTGGACCAGATGGGTGTGGTGACGTGGACAAGGAGAGGATTAACCACGTGTGCTTTAGTAGGGATGGGCAGTGGCTCTTATTTACGTCGAATTTGGGTGGGGTGACAGCGGAGCCAGTGTCGCTGCCCAATCAGTTTCAGCCATATGGAGATTTGTTTGTGGTGAGGTTGGATGGGACCGGGCTAAGGAGGCTGACGTGGAATGGATATGAAAATGGAACTCCCACGTGGTATTATGGAAGTGAGCTGGCACTTTCTGGGTTGAGTTTGAAAGATAATGTGGTTGGTGAGAAGTTGGCGGGAAAGTTTGTTGAACCGCTTTGGATAACGTTCCATTAA

Coding sequence (CDS)

ATGGACAACATCCCCACCGGCACCGTCATCTTCACCACCGTCGACCGCCTCCAATACGGCTTCGACACCTTCTCTGTTCCCATCAATTCCCCCACCGTCGAACATCGCCTCACCGACGGAATCTCCGTCAATTTCAACGCCCAATTCCTCCAAAACGACCAATCCATTGTCTTCATCTCTGAACGATCCGGCTCCCCTAGAATTTACCTCTCAAATTCCCCCGATTCCGCCCCTAAATTGCTCCCCTCCGCTCCCGGAAGCTGCTTTCACGACCGACCCATCGTCAGAAATGGTCGCCTCTATTTCATCTCCGCTCATGAAAACCCCCACAAGCCCTTAACGAGCTGGTCCGCTCTGTATTCGACCGGGTTGGACGGCTCCGATCCGGTGACCCGGCTCACTCCTCCTGGGTCCGTCGATTTCAGTCCAGCGGTTTCTGAGAGTGGGAAGTTCTTGGCGGTTGCCTCTTATGGATCTCGTTCTTGGGGCGGCGAATTCAACGAGCTCCACACGGAGATTGTTGTGTTCAAAGCTTCCGACCCGACTGGTCGGGTCGTTGTTTCGTGTCGCGGCGGATGGCCGTCGTGGTCTGGCGACTCCGCTGTTTACTTCCACCGGGTGGCCGACGATGGGTGGTGGAGCATTTTCAGGGTGGAGATTCCCGAAAATCTCGATTCCTCTGTTTCCCCTGTTCCGATCCGGGTCACTCCGGCGGGTCTCCATTGCTTCACTCCAGCGGCCATGAACGACTGCAATCGAGTCGTCGTCGCCACCCGAAGACCCGACAGTAAATTCCGACACATCGAAATTTTCGATTCCGGGTCGGACGAATTCGTCCCGATTACCCAGAAATTGAATCCGAATTTCCATCACTACAACCCGTTCGTCTCGCCGGATTCCAATTACGTCGGGTACCACCGGTTCCGCGGCGAGTCAAGTCAGGGCGAGTTAACGATTCCACATCTCAACTCGGTAATTTCCCCGATTAATGAACTCCGTATGATCCGATTAAACGGTTCGTTCCCAACGCCGTCACCTGACGGCGATTTCATCGCGTTCAATCCCGATTTTGTGGGGTTAAAAATCGTCAAATCAGACGGCTCAAAATGTTGGACCGTACTGAAAGAACGTACTGCGTTTTACAATTCATGGAGCCCCACGGAAAAAAACGTGATTTACACTTCGCTGGGGCCCATTTTTGGGCCACCGAGAGCGACGGTCCAGATCGCTCGGATCACAATCAACTCCGACGATCTCAACGGTGGTGATAACGAGGAAGTCGCCAGTGAAGTGAAGATTCTGACGAAAGACGACAGCGGAAATAACGCATTTCCAGCGTGTTCGCCGGACGGGAAGTTTCTAGTTTTCCGGTCCGGCAGGTCCGGGCATAAGAATCTTTACATCGTCGACGCCGTGAACGGCGAGTTCGACGGCGGCATACGGCGGTTGACCGACGGACCGTGGATCGACACAATGCCGAGCTGGTCGCCGACGGGGGATCTGATCGCGTTTTCTTCGAATATGCATAATCCGAAAAATAGTGAAGTGTTTAGTATCTACGTCATCAGACCGGACGGTTCGGGTTTGCGAAGGGTACACGTGGCTGGACCAGATGGGTGTGGTGACGTGGACAAGGAGAGGATTAACCACGTGTGCTTTAGTAGGGATGGGCAGTGGCTCTTATTTACGTCGAATTTGGGTGGGGTGACAGCGGAGCCAGTGTCGCTGCCCAATCAGTTTCAGCCATATGGAGATTTGTTTGTGGTGAGGTTGGATGGGACCGGGCTAAGGAGGCTGACGTGGAATGGATATGAAAATGGAACTCCCACGTGGTATTATGGAAGTGAGCTGGCACTTTCTGGGTTGAGTTTGAAAGATAATGTGGTTGGTGAGAAGTTGGCGGGAAAGTTTGTTGAACCGCTTTGGATAACGTTCCATTAA

Protein sequence

MDNIPTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSGSPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGLDGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGRVVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFTPAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVGYHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVKSDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDNEEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGYENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH
Homology
BLAST of Lag0002696 vs. NCBI nr
Match: XP_022954120.1 (uncharacterized protein LOC111456480 [Cucurbita moschata])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 561/642 (87.38%), Postives = 600/642 (93.46%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP++SPT EH LTDGISVNFNAQF+ N QSIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGSD VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           VVVS RGGWPSWSGDS VYFHR A+DGWWSIFRVEIPENLDSSV PVPIRVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDS Y+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSKYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GELTIPH   +ISPINELR+IR+NGSFPT SPDG+FIAFNPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKCWTVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GD+
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EVA EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F+G  RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           DGPWIDTMPSWSP GDLIAFSSNMHNP+N+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. NCBI nr
Match: KAG6575727.1 (hypothetical protein SDJN03_26366, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 560/642 (87.23%), Postives = 600/642 (93.46%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP++SPT EH LTDGISVNFNAQF+ N QSIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGSD VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           VVVS RGGWPSWSGDS VYFHR A+DGWWSIFRVEIPENLDSSV PV +RVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVAVRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDSNY+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GELTIPH   +ISPINELR+IR+NGSFPT SPDG+FIAFNPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKCWTVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GD+
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EVA EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F+G  RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           DGPWIDTMPSWSP GDLIAFSSNMHNP+N+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. NCBI nr
Match: XP_023547812.1 (uncharacterized protein LOC111806666 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 562/642 (87.54%), Postives = 598/642 (93.15%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP+NSPT EH LTDGISVNFNAQF+ N  SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLNSPTTEHCLTDGISVNFNAQFVDNQLSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGSD VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSDSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           VVVS RGGWPSWSGDS VYFHR A+DGWWSIFRVEIPENLDSSV PVPIRVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDSNY+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GELTIPH   +ISPINELRMIR+NGSFPT SPDG+FIAFNPDFVGL+IVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRMIRINGSFPTQSPDGNFIAFNPDFVGLRIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKC TVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GD+
Sbjct: 364 ADGSKCVTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EVA EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F G  RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFGGEARRLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           DGPWIDTMPSWSP GDLIAFSSNMHNPKN+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPKNTEAFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. NCBI nr
Match: KAG7014283.1 (tolB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 558/642 (86.92%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP++SPT EH LTDGISVNFNAQF+ N QSIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGSD VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           VVVS RGGWPSWSGDS VYFHR A+DGWWSIF VEIPENLDSSV PV +RVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFSVEIPENLDSSVPPVAVRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDSNY+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GELTIPH   +ISPINELR+IR+NGSFPT SPDG+FIAFNPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKCWTVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GD+
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EVA EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F+G  RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           DGPWIDTMPSWSP GDLIAFSSNMHNP+N+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVV+LDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVKLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDAVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. NCBI nr
Match: XP_022991715.1 (uncharacterized protein LOC111488250 [Cucurbita maxima])

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 555/642 (86.45%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP++SPT EH LTDGISVNFNAQF+ N QSIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYVTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGS  VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSVSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           V+VS RGGWPSWSGDS V+FHR A+DGWWSIFR EIPENLDSSV PVPIRVTPAGLHCFT
Sbjct: 184 VIVSGRGGWPSWSGDSTVFFHRQAEDGWWSIFRGEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDSNY+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GE+TIPH   +ISPINELR+IR+NGSFPT SPDG+FIAFNPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGEITIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKCWTVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GDN
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDN 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EV+ EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F+G  R+LT
Sbjct: 424 DEVSGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARQLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           +GPWIDTMPSWSP GDLIAFSSNMHNP+N+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 NGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. ExPASy Swiss-Prot
Match: Q3A097 (Tol-Pal system protein TolB OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=tolB PE=3 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 6.3e-16
Identity = 76/278 (27.34%), Postives = 124/278 (44.60%), Query Frame = 0

Query: 360 LKIVKSDGSKCWTVLKERTAFYN-SWSPTEKNVIYTSL----GPIFGPPRATVQIARIT- 419
           L ++ +DG+    +   R+   N  +SP  + VIYTS       ++    +T Q AR+  
Sbjct: 181 LYLMDTDGANALRLTDHRSIVLNPDFSPNGREVIYTSYRRGNPDLYRKQLSTGQEARLAF 240

Query: 420 ---INSDDLNGGDNEEVA--------SEVKILTKDDS---------GNNAFPACSPDGKF 479
              +N       D  E+A         E+ +L  D S         G +  P+ SP G  
Sbjct: 241 KKGLNIAARFRPDGREIALTQSATGNPELMLLGTDGSRRRRLTSHWGIDVDPSWSPAGDR 300

Query: 480 LVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKN 539
           L F S R G+ +++++D + G+      RLT     +  P+WSP G  IAF+        
Sbjct: 301 LAFVSDRQGNPHIFVMDLLGGQ----TARLTANGKYNATPAWSPDGKRIAFTR-----LE 360

Query: 540 SEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRDGQWLLFTSNLGGVTAEP 599
             VF IY +RPDG+  RR+        G  +KE   H  +S D ++L+++S+  G     
Sbjct: 361 KGVFDIYTVRPDGTDERRLTF------GPGNKE---HPRWSPDSRFLVYSSDQDGRKG-- 420

Query: 600 VSLPNQFQPYGDLFVVRLDGTGLRRLTWNGYENGTPTW 612
                       ++++R DGTG+RR++  G +   P W
Sbjct: 421 ------------IYIMRADGTGIRRISAGGGQCQHPAW 426

BLAST of Lag0002696 vs. ExPASy Swiss-Prot
Match: Q3APB5 (Protein TolB homolog OS=Chlorobium chlorochromatii (strain CaD3) OX=340177 GN=tolB PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.4e-15
Identity = 79/291 (27.15%), Postives = 118/291 (40.55%), Query Frame = 0

Query: 329 INELRMIRLNGSFPTPSPDGDFIAFNPDFVG----LKIVKSDGSKCWTVLKERTAFYNSW 388
           +++L   R     P  SPDG ++AF  DF G    L I +    K   V K+  +   +W
Sbjct: 201 VHQLTNFRSIALTPALSPDGAYLAFT-DFTGGKPALAIRELATGKTTRVAKKGNSIDPAW 260

Query: 389 SPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDNE----EVASEVKILTKDDSG 448
             + +     S                          GD E    + A  VK      SG
Sbjct: 261 RNSRELATTFSF------------------------EGDQELYLLDSAGAVKQRLTSSSG 320

Query: 449 NNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSPTGD 508
            +  P  SPDG+ + F S RSG+  +++ D  +G+     +RLT     +T P+WSP GD
Sbjct: 321 IDLSPTFSPDGRKMAFVSARSGNPQIFVYDFSSGK----SQRLTFSGRYNTQPAWSPIGD 380

Query: 509 LIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRDGQWL 568
            IAFS+     ++    +I+VI  DGSGL ++     +              +S DG+ +
Sbjct: 381 KIAFST----WESGGEINIFVINTDGSGLTQLTTQSGEN---------ESPSWSPDGRMI 435

Query: 569 LFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGYENGTPTW 612
           +F SN  GV                L+V+  DG   R L   G E   P+W
Sbjct: 441 VFASNRQGVK--------------KLYVMMADGKNQRPLLAIGGEQTQPSW 435

BLAST of Lag0002696 vs. ExPASy Swiss-Prot
Match: A8LHQ6 (Tol-Pal system protein TolB OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) OX=398580 GN=tolB PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.9e-13
Identity = 69/222 (31.08%), Postives = 95/222 (42.79%), Query Frame = 0

Query: 395 SLGPIFGPP-RATVQIARITINSD----DLNGGDNEEVASEVKILTKDDSGNNAFPACSP 454
           S  P F P  R+ V       NSD    DL+ G    + +   I T         P+ SP
Sbjct: 259 SFAPRFSPDGRSVVFSLTNGGNSDIYRRDLSSGAQTRLTATPAIETA--------PSFSP 318

Query: 455 DGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMH 514
           DG+ +VF S RSG + LY++ A  GE     RR++ GP     P WSP GDLIAF+    
Sbjct: 319 DGRQIVFESDRSGSQQLYVMSATGGE----ARRISFGPGRYGTPVWSPRGDLIAFTK--- 378

Query: 515 NPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRDGQWLLFTSNLGGV 574
             +N   F I V+R DGS  R +  +  D              +S +G+ ++FT    G 
Sbjct: 379 --QNQGRFHIGVMRTDGSEERLLTSSFLD----------ESPTWSPNGRVIMFTRETSGA 438

Query: 575 TAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGYENGTPTW 612
              P            L+ V + G  LRR+   G  +  P W
Sbjct: 439 GGAP-----------SLYSVDISGRNLRRVPTPGAAS-DPAW 441

BLAST of Lag0002696 vs. ExPASy Swiss-Prot
Match: Q0AC40 (Tol-Pal system protein TolB OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=tolB PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 8.6e-13
Identity = 70/249 (28.11%), Postives = 106/249 (42.57%), Query Frame = 0

Query: 360 LKIVKSDGSKCWTVLKERTAFYN-SWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDD 419
           L +  +DG +   +L+ R    + +WSP    + Y S            +  R  I   +
Sbjct: 183 LMVADADGQRPREILESRQPIMSPAWSPERDRLAYVSF-----------EGGRSEIYVQN 242

Query: 420 LNGGDNEEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDG 479
           L  G  + +AS          G N+ PA SPDG+ L     R G  N+Y++       DG
Sbjct: 243 LRSGQRDRIAS--------FRGINSAPAWSPDGRHLAVTLSRDGRANIYLLRL----DDG 302

Query: 480 GIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPD 539
            +RRLTD   IDT P++SP G+ IAF+S+       +V+++ V  P   G+ RV   G  
Sbjct: 303 HVRRLTDHWAIDTEPTFSPDGERIAFTSDRGG--RPQVYTLAVNGP--GGVERVTFDGDY 362

Query: 540 GC---GDVDKERINHVCFSRDGQWLLFTSNLGG-----VTAEPVSLPNQFQPYGDLFVVR 599
                   D  RI  V    +G + +   +L       +T  P      F P GD+ +  
Sbjct: 363 NARPNWSPDGRRIAMV-HRHNGSFRIAVHDLESDRTRVLTDGPWDESPVFAPNGDMIMYS 403

BLAST of Lag0002696 vs. ExPASy Swiss-Prot
Match: Q167Z6 (Tol-Pal system protein TolB OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=tolB PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.1e-12
Identity = 62/193 (32.12%), Postives = 87/193 (45.08%), Query Frame = 0

Query: 395 SLGPIFGPPRATV-----QIARITINSDDLNGGDNEEVASEVKILTKDDSGNNAFPACSP 454
           S  P F P   T+     Q     I   ++NGG    + S   I T         P+ SP
Sbjct: 256 SFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIETA--------PSYSP 315

Query: 455 DGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMH 514
           DG  +VF S RSG + LY++ A  GE     RR++ GP     P WSP GDL+AF+    
Sbjct: 316 DGTQIVFESDRSGSQQLYVMPANGGE----ARRISFGPGRYGTPVWSPRGDLVAFTK--- 375

Query: 515 NPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRD-----GQWLLFTS 574
             +N+  F I V+R DGS  R +  +  D  G         + FSR+     G+  L++ 
Sbjct: 376 --QNAGRFHIGVMRLDGSEERLLTASFLDE-GPTWSPNGRVIMFSRETQGAQGRATLYSV 430

Query: 575 NLGGVTAEPVSLP 578
           ++ G    PV  P
Sbjct: 436 DITGRNLRPVRTP 430

BLAST of Lag0002696 vs. ExPASy TrEMBL
Match: A0A6J1GRK1 (uncharacterized protein LOC111456480 OS=Cucurbita moschata OX=3662 GN=LOC111456480 PE=4 SV=1)

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 561/642 (87.38%), Postives = 600/642 (93.46%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP++SPT EH LTDGISVNFNAQF+ N QSIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGSD VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           VVVS RGGWPSWSGDS VYFHR A+DGWWSIFRVEIPENLDSSV PVPIRVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDS Y+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSKYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GELTIPH   +ISPINELR+IR+NGSFPT SPDG+FIAFNPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKCWTVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GD+
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EVA EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F+G  RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           DGPWIDTMPSWSP GDLIAFSSNMHNP+N+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. ExPASy TrEMBL
Match: A0A6J1JTR3 (uncharacterized protein LOC111488250 OS=Cucurbita maxima OX=3661 GN=LOC111488250 PE=4 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 555/642 (86.45%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFISERSG 64
           PTGTVIFTTV R QYGFDTFSVP++SPT EH LTDGISVNFNAQF+ N QSIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 65  SPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGL 124
           SPR+YLSNSP+SAPKLLPSAPGSCFHDRPI+RN RLYFISAHENPHKP TSWSALY TGL
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYVTGL 123

Query: 125 DGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGR 184
           DGS  VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF ELHTEIVVF++SDP  R
Sbjct: 124 DGSVSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 185 VVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVSPVPIRVTPAGLHCFT 244
           V+VS RGGWPSWSGDS V+FHR A+DGWWSIFR EIPENLDSSV PVPIRVTPAGLHCFT
Sbjct: 184 VIVSGRGGWPSWSGDSTVFFHRQAEDGWWSIFRGEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 245 PAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVG 304
           PAAMND  RVVVATRRPD+KFRHIEI++SG+DEF PITQKLNP+FHHYNPFVSPDSNY+G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 305 YHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVK 364
           YHRFRGESS GE+TIPH   +ISPINELR+IR+NGSFPT SPDG+FIAFNPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGEITIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 365 SDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDN 424
           +DGSKCWTVLK+RTAFYNSWSPTEKNVIY+SLGPIFGP RATVQIAR TINSDDLN GDN
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDN 423

Query: 425 EEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLT 484
           +EV+ EVKILTK+D+GNNAFPACSPDGKFLVFRSGRSGHKNLYI+DAVNG+F+G  R+LT
Sbjct: 424 DEVSGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARQLT 483

Query: 485 DGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVD 544
           +GPWIDTMPSWSP GDLIAFSSNMHNP+N+E FSIYVIRPDGS LRRVHVAGP+G  DVD
Sbjct: 484 NGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 545 KERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGY 604
           KERINHVCFSRDG+WLLFTSNLGGV+AEPVS+PNQFQPYGDLFVVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 605 ENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITFH 647
           ENGTPTWYYGSELALSGLSLKD VVGEKL G+F EPLWITF+
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of Lag0002696 vs. ExPASy TrEMBL
Match: A0A5A7URS2 (TolB protein-related isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00020 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 541/648 (83.49%), Postives = 587/648 (90.59%), Query Frame = 0

Query: 1   MDNIPTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFIS 60
           MDN PTG V+FTT+   QYGFD FSV +NSPTVE RLTDGISVNFNAQFL N  S+VFIS
Sbjct: 1   MDN-PTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFIS 60

Query: 61  ERSGSPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALY 120
           ERSGS RIYLS+SP+S+PKLL SAPGSCFHDRPIVRNGRL FISAHENPHKP TSW+ALY
Sbjct: 61  ERSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALY 120

Query: 121 STGLDGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASD 180
           ST LDG D +TRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF+EL+ EIVVFK+SD
Sbjct: 121 STPLDGDDSITRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSD 180

Query: 181 PTGRVVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSV---SPVPIRVTP 240
           P  RVVV+ RGGWPSWSGDS V+FHR A+DGWWSIF+VEIPENLDSS+   SPVPIRVTP
Sbjct: 181 PDRRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTP 240

Query: 241 AGLHCFTPAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVS 300
           AGLHCFTPAAMND   VVVATRR DSKFRHIEIFDS  +EF+PITQKLNP+FHHYNPFVS
Sbjct: 241 AGLHCFTPAAMNDSRLVVVATRRADSKFRHIEIFDSELEEFIPITQKLNPDFHHYNPFVS 300

Query: 301 PDSNYVGYHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDF 360
           PDSN++GYHRFRGES+Q EL IP+L+ VISPI EL+MIR+NGSFPTPSPDGD IAFNP+F
Sbjct: 301 PDSNFIGYHRFRGESTQSEL-IPYLSPVISPIKELQMIRINGSFPTPSPDGDLIAFNPNF 360

Query: 361 VGLKIVKSDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSD 420
            GL+IVK DGSKC TVLK+RTAFYNSWSPTEKNVIYTSLGPIFG   ATVQIARITINSD
Sbjct: 361 NGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITINSD 420

Query: 421 DLNGGDNEEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFD 480
           DL  GD++EV+SEVKILTKDD+GNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAV GEF+
Sbjct: 421 DLKDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKGEFE 480

Query: 481 GGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGP 540
           G +RRLTDG WIDTMP+WSP GDLI FSSNMHNPKN+E FSIYVIRPDGSGLRRV+VAGP
Sbjct: 481 GELRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGP 540

Query: 541 DGCGDVDKERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLR 600
           +G  +VD+ERINHVCFSRDG+WLLFT+NL GVTAEPVS PNQFQPYGDLFVVRLDGTGLR
Sbjct: 541 EGSSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGTGLR 600

Query: 601 RLTWNGYENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITF 646
           RLTWNGYENGTPTWYYGSE+ALSGLSLKD VVGEKL G F EPLWITF
Sbjct: 601 RLTWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITF 646

BLAST of Lag0002696 vs. ExPASy TrEMBL
Match: A0A1S3CEJ8 (uncharacterized protein LOC103499982 OS=Cucumis melo OX=3656 GN=LOC103499982 PE=4 SV=1)

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 538/648 (83.02%), Postives = 584/648 (90.12%), Query Frame = 0

Query: 1   MDNIPTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFIS 60
           MDN PTG V+FTT+   QYGFD FSV +NSPTVE RLTDGISVNFNAQFL N  S+VFIS
Sbjct: 1   MDN-PTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFIS 60

Query: 61  ERSGSPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALY 120
           ERSGS RIYLS+SP+S+PKLL SAPGSCFHDRPIVRNGRL FISAHENPHKP TSW+ALY
Sbjct: 61  ERSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALY 120

Query: 121 STGLDGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASD 180
           ST LDG D +TRLTP GSVDFSPAVS SGKF+AVASYGSRSWGGEF+EL+ EIVVFK+SD
Sbjct: 121 STPLDGDDSITRLTPLGSVDFSPAVSGSGKFVAVASYGSRSWGGEFHELNLEIVVFKSSD 180

Query: 181 PTGRVVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSV---SPVPIRVTP 240
           P  RVVV+ RGGWPSWSGDS V+FHR A+DGWWSIF+VEIPENLDSS+   SPVPIRVTP
Sbjct: 181 PDRRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTP 240

Query: 241 AGLHCFTPAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVS 300
           AGLHCFTPAAMND   VVVATRR DSKFRHIEIFDS  +EF+PITQKLNP+FHHYNPFVS
Sbjct: 241 AGLHCFTPAAMNDSRLVVVATRRADSKFRHIEIFDSELEEFIPITQKLNPDFHHYNPFVS 300

Query: 301 PDSNYVGYHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDF 360
           PDSN++GYHRFRGES+Q EL IP+L+ VISPI EL+MIR+NGSFP PSPDGD IAFNP+F
Sbjct: 301 PDSNFIGYHRFRGESTQSEL-IPYLSPVISPIKELQMIRINGSFPAPSPDGDLIAFNPNF 360

Query: 361 VGLKIVKSDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSD 420
            GL+IVK DGSKC TVLK+RTAFYNSWSPTEKNVIYTSLGPIFG   ATVQIARITINSD
Sbjct: 361 NGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITINSD 420

Query: 421 DLNGGDNEEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFD 480
           DL  GD++EV+SEVKILTKDD+GNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAV GEF+
Sbjct: 421 DLKDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKGEFE 480

Query: 481 GGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGP 540
           G +RRLTDG WIDTMP+WSP GDLI FSSNMHNPKN+E FSIYVIRPDGSGLRRV+VAGP
Sbjct: 481 GELRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGP 540

Query: 541 DGCGDVDKERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLR 600
           +G  +VD+ERINHVCFSRDG+WLLFT+NL GVTAEPVS PNQFQPYGDLFVVRLDGTGL 
Sbjct: 541 EGSSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGTGLS 600

Query: 601 RLTWNGYENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITF 646
           RLTWNGYENGTPTWYYGSE+ALSGLSLKD VVGEKL G F EPLWITF
Sbjct: 601 RLTWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITF 646

BLAST of Lag0002696 vs. ExPASy TrEMBL
Match: A0A0A0K6G2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G419590 PE=4 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 532/648 (82.10%), Postives = 578/648 (89.20%), Query Frame = 0

Query: 1   MDNIPTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQSIVFIS 60
           MDN PTG V+FTTV   QYGFD FSVP+NS TVE +LTDGISVNFNAQFL N  S+VFIS
Sbjct: 1   MDN-PTGAVLFTTVGLNQYGFDIFSVPLNSLTVERQLTDGISVNFNAQFLNNQLSVVFIS 60

Query: 61  ERSGSPRIYLSNSPDSAPKLLPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALY 120
           ERSGS RIYLS+SP+S+PKLL SAPGSCFHDRPIV NGRL FISAHENPHKP TSW+ALY
Sbjct: 61  ERSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVTNGRLLFISAHENPHKPFTSWAALY 120

Query: 121 STGLDGSDPVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASD 180
           ST LDG D VTRLTP GSVDFSPAVSESGKF+AVASYGSRSWGGEF+EL+ EIVVFK+SD
Sbjct: 121 STPLDGHDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSD 180

Query: 181 PTGRVVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLD---SSVSPVPIRVTP 240
           P  RVVV+ RGGWPSWSGDS V+FHR ADDGWWSIF+VEIPENLD   SSVSPV IRVTP
Sbjct: 181 PGQRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSSRSSVSPVAIRVTP 240

Query: 241 AGLHCFTPAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVS 300
           AGLHCFTPAAMND  RVVVATRR DSK+RHIEIFDS  +EF+PITQKLNP FHHYNPFVS
Sbjct: 241 AGLHCFTPAAMNDGRRVVVATRRADSKYRHIEIFDSELEEFIPITQKLNPEFHHYNPFVS 300

Query: 301 PDSNYVGYHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDF 360
           PDSN++GYHRFRGES+Q EL IP+L  VISPI EL++IR+NGSFPTPSPDGD IAFNP F
Sbjct: 301 PDSNFIGYHRFRGESTQSEL-IPYLYPVISPIKELQIIRVNGSFPTPSPDGDLIAFNPGF 360

Query: 361 VGLKIVKSDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSD 420
           +GL+IVK DGSKC TVLK+RTAF NSWSPTEKNVIYTSLGPIFG   ATVQIARITINS 
Sbjct: 361 IGLQIVKFDGSKCRTVLKDRTAFCNSWSPTEKNVIYTSLGPIFGAVTATVQIARITINS- 420

Query: 421 DLNGGDNEEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFD 480
               GD++EV++EVKILTKD++GNNAFPACSPDGKFLVFRSGR+GHKNLYIVDA+ GEF+
Sbjct: 421 ----GDSDEVSNEVKILTKDNTGNNAFPACSPDGKFLVFRSGRTGHKNLYIVDAMKGEFE 480

Query: 481 GGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGP 540
           G +R+LTDGPWIDTMP+WSP GDLI FSSNMHNPKN+E FSIYVIRPDGSGLRRV+VAGP
Sbjct: 481 GELRQLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGP 540

Query: 541 DGCGDVDKERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLR 600
           +G  +VD+ERINHVCFSRDG WLLFT+NL GVTAEPVSLPNQFQPYGDLFVVRLDGTGLR
Sbjct: 541 EGSSEVDRERINHVCFSRDGNWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGLR 600

Query: 601 RLTWNGYENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWITF 646
           RLT N YENGTPTWYYGSELALSGLSLKD VVGEKL G F EPLWI F
Sbjct: 601 RLTCNAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGDFDEPLWIKF 641

BLAST of Lag0002696 vs. TAIR 10
Match: AT4G01870.1 (tolB protein-related )

HSP 1 Score: 883.2 bits (2281), Expect = 1.2e-256
Identity = 421/650 (64.77%), Postives = 518/650 (79.69%), Query Frame = 0

Query: 5   PTGTVIFTTVDRLQYGFDTFSVPINSPTVEHRLTDGISVNFNAQFLQNDQS--IVFISER 64
           P GT+IFTTV R  YGFD FS+ I + +VE RLTDG+SVNFNAQF+ ND+S  +VF+SER
Sbjct: 4   PKGTIIFTTVGRTHYGFDVFSLNI-ATSVERRLTDGVSVNFNAQFV-NDKSDDVVFVSER 63

Query: 65  SGSPRIYLSNSPDSAPKLLPSAPGSCFHDRPIV-RNGRLYFISAHENPHKPLTSWSALYS 124
           +GS RIY + S  S P+ +P AP S FHDRPI+ +N RLYFISAHE P +   +WSALY+
Sbjct: 64  NGSARIYKTRSGISKPEQIPGAPESYFHDRPIITQNNRLYFISAHEQPDRYFKNWSALYT 123

Query: 125 TGLDGSD-PVTRLTPPGSVDFSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASD 184
             L+ +   VTR+TPP + DFSPAVS+SG FLAVASYG+RSWGGEF+E++T+I VFKAS 
Sbjct: 124 VELNSAKREVTRVTPPDTADFSPAVSQSGDFLAVASYGTRSWGGEFHEINTDITVFKASK 183

Query: 185 PTGRVVVSCRGGWPSWSGDSAVYFHRVADDGWWSIFRVEIPENLDSSVS-PV-PIRVTPA 244
           P  RVV+  RGGWP+WSGDS V+FH  ADDGWWSIFRV+IPEN       P+ PIRVTP+
Sbjct: 184 PETRVVICERGGWPTWSGDSTVFFHHQADDGWWSIFRVDIPENFTEYTDFPITPIRVTPS 243

Query: 245 GLHCFTPAAMNDCNRVVVATRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSP 304
           GLHCFTPAA  D  R+ +ATRR     RHIEI+D  +  F P+T+ LNP+FHHYNPFVSP
Sbjct: 244 GLHCFTPAAFRDGKRIALATRRRGVNHRHIEIYDLENTTFQPVTESLNPSFHHYNPFVSP 303

Query: 305 DSNYVGYHRFRGESSQGELTIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDF- 364
           DS ++GYHRFRGES+QGE  +P++ S++SPI  LR++R+NGSFP+ SP+GD IA N DF 
Sbjct: 304 DSEFLGYHRFRGESTQGESIVPNIESIVSPIKTLRLLRINGSFPSSSPNGDLIALNSDFD 363

Query: 365 --VGLKIVKSDGSKCWTVLKERTAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITIN 424
              G+K+ KSDGSK WT++K+RTAFYNSWSPTE++VIYTSLGPIF P R  VQIARI  +
Sbjct: 364 INGGIKVSKSDGSKRWTLIKDRTAFYNSWSPTERHVIYTSLGPIFSPARIAVQIARIKFD 423

Query: 425 SDDLNGGDNEEVASEVKILTKDDSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGE 484
             DL   D E++  +VKILT +++GNNAFP+CSPDGK +VFRSGRSGHKNLYIVDAVNGE
Sbjct: 424 PSDLT-ADKEDLPCDVKILTLENTGNNAFPSCSPDGKSIVFRSGRSGHKNLYIVDAVNGE 483

Query: 485 FD-GGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHV 544
            + GGIRRLTDGPWIDTMP WSP GDLI FSSN HNP+N+ VF  YV+RPDG+GLRR+ +
Sbjct: 484 SNGGGIRRLTDGPWIDTMPCWSPKGDLIGFSSNRHNPENTAVFGAYVVRPDGTGLRRIQI 543

Query: 545 AGPDGCGDVDKERINHVCFSRDGQWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGT 604
           +GP+G  +  +ER+NHV F++DG WL+F +NL GVTAEPV++PNQFQPYGDL+VV+LDGT
Sbjct: 544 SGPEGSEEAARERVNHVSFNKDGDWLVFAANLSGVTAEPVTMPNQFQPYGDLYVVKLDGT 603

Query: 605 GLRRLTWNGYENGTPTWYYGSELALSGLSLKDNVVGEKLAGKFVEPLWIT 645
           GLRRLTWNGYE+GTPTW+   EL LS L+L +   G+KL G+F EPLWI+
Sbjct: 604 GLRRLTWNGYEDGTPTWHTADELDLSQLNL-NGQDGDKLEGQFEEPLWIS 649

BLAST of Lag0002696 vs. TAIR 10
Match: AT1G21680.1 (DPP6 N-terminal domain-like protein )

HSP 1 Score: 483.4 bits (1243), Expect = 2.8e-136
Identity = 274/654 (41.90%), Postives = 382/654 (58.41%), Query Frame = 0

Query: 8   TVIFTTVDRLQYGFDTFSVPINSP---TVEHRLTDGISVNFNAQF--------------- 67
           T+IFTT+ R  Y FD F++    P   + E R+TDG SVNFN  F               
Sbjct: 33  TIIFTTLGRSHYEFDIFALSTTQPPSVSGELRITDGESVNFNGYFPSPSPALLSLLPDET 92

Query: 68  -LQNDQS----IVFISERSGSPRIYL-------------------SNSPDSAPKL----- 127
            +Q + S    +++++ER+G+  +Y                    + S    P L     
Sbjct: 93  LIQMEDSSPLHLIYVTERNGTSSLYYDLVYGGNSDFKTKRRSVLEAPSRVQVPLLSRFDH 152

Query: 128 LPSAPGSCFHDRPIVRNGRLYFISAHENPHKPLTSWSALYSTGLDGSDPVTRLTPPGSVD 187
           L     + F D+P +    + ++S HE+  +P  SW+A+YST L  +    RLTP G  D
Sbjct: 153 LSGMTVNSFKDKPSLSGEFIVYVSTHESSGEPRASWTAVYSTELK-TGLTRRLTPSGVAD 212

Query: 188 FSPAVSESGKFLAVASYGSRSWGGEFNELHTEIVVFKASDPTGRVVVSCRGGWPSWSGDS 247
           FSPAVS SG   AVASYG R W GE  EL T+I VF   D + RV V   GGWP W  +S
Sbjct: 213 FSPAVSPSGNLTAVASYGERGWTGEVEELRTDIYVFLTRDGSHRVKVVEHGGWPCWVDES 272

Query: 248 AVYFHRVA-DDGWWSIFRVEIPENLDSSVSPVPI-RVTPAGLHCFTPA-AMNDCNRVVVA 307
            +YFHR + +DGW S++R  +PEN   +   V I RVTP G+H FTPA + N+   V VA
Sbjct: 273 TLYFHRRSEEDGWISVYRAILPENGPLTTESVTIQRVTPPGVHAFTPATSPNNHEFVAVA 332

Query: 308 TRRPDSKFRHIEIFDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVGYHRFRGESSQGEL 367
           TRRP S +RH+E+FD   +EF+ +T+ + P  HH NPF+SPDS+ VGYH  RG+++    
Sbjct: 333 TRRPGSDYRHVELFDLKRNEFIELTRLVAPKSHHLNPFLSPDSSRVGYHSCRGDANGRRS 392

Query: 368 TIPHLNSVISPINELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVKSDGSKCWTVLKER 427
            +  L ++ +   +L + R++GSFP+ SP GD IA+     G+ +VK DGS    V K  
Sbjct: 393 PLLFLENIQTTTRDLSLFRIDGSFPSFSPGGDRIAY-VKMPGVFVVKPDGSGQREVYK-G 452

Query: 428 TAFYNSWSPTEKNVIYTSLGPIFGPPRATVQIARITINSDDLNGGDNEEVASEVKILTKD 487
            AF  +W P    ++Y+S GP F   R  V +  I +++ D         +S V+ LT +
Sbjct: 453 MAFSTAWDPVRPGIVYSSSGPTFATERTEVDVISIDVDAAD--------KSSSVRRLTTN 512

Query: 488 DSGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSP 547
              NNAFP  SPDGK +VFRSGR+GHKNLYI+DA  GE  GG+ RLT+G W DTM +WSP
Sbjct: 513 GK-NNAFPWPSPDGKRIVFRSGRTGHKNLYIMDAEKGE-SGGLWRLTEGAWTDTMCNWSP 572

Query: 548 TGDLIAFSSNMHNPKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRDG 607
            G+ IAF+S+  +P  S  F +++I P+G+GLR++  +G  G       R NH  FS D 
Sbjct: 573 DGEWIAFASDRESP-GSGSFELFLIHPNGTGLRKLIQSGTGG-------RTNHPIFSPDS 632

Query: 608 QWLLFTSNLGGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGYENGTPTW 612
           + L+FTS+  G++AEP+S P+ +QPYGD+F V+LDG+ +RRLT N YE+GTP W
Sbjct: 633 KSLVFTSDYAGISAEPISNPHHYQPYGDIFTVKLDGSNVRRLTHNSYEDGTPAW 665

BLAST of Lag0002696 vs. TAIR 10
Match: AT1G21670.1 (LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: WD40-like Beta Propeller (InterPro:IPR011659), Six-bladed beta-propeller, TolB-like (InterPro:IPR011042); BEST Arabidopsis thaliana protein match is: DPP6 N-terminal domain-like protein (TAIR:AT1G21680.1); Has 8461 Blast hits to 5060 proteins in 1257 species: Archae - 79; Bacteria - 5567; Metazoa - 37; Fungi - 70; Plants - 117; Viruses - 0; Other Eukaryotes - 2591 (source: NCBI BLink). )

HSP 1 Score: 462.6 bits (1189), Expect = 5.1e-130
Identity = 263/642 (40.97%), Postives = 374/642 (58.26%), Query Frame = 0

Query: 8   TVIFTTVDRLQYGFDTFSVPIN----SPTVEHRLTDGISVNFNAQFLQ------------ 67
           T++FTT+ R  + FD F++P +    SP  EHRLTDG S+NFN  F              
Sbjct: 33  TILFTTIGRPTFEFDIFTLPTSHRPPSPADEHRLTDGKSINFNGYFASPSTALISLLPKR 92

Query: 68  -----NDQSIVFISERSGSPRIY--LSNSPDSAPKL-LPSAPG---------SCFHDRPI 127
                 D  +++++ER+G+P +   + +S +   ++ +P   G         +   D P+
Sbjct: 93  TQIQPQDVHLIYVTERAGTPSLNYDVVHSDNVGSRIQVPLFSGEEQQSGMNVNSMKDTPV 152

Query: 128 VRNGRLYFISAHENPHKPLTSWSALYSTGLDGSDPVTRLTPPGSVDFSPAVSESGKFLAV 187
           + NG L  +S HENP KP+ SW+A+YST L  +    RLTP G  DFSPAVS SGK+ AV
Sbjct: 153 LTNGYLVHVSTHENPGKPMASWAAVYSTEL-RTKSTRRLTPLGIADFSPAVSPSGKWTAV 212

Query: 188 ASYGSRSWGGEF--NELHTEIVVFKASDPTGRVVVSCRGGWPSWSGDSAVYFHRVADDGW 247
           AS+G + W       E+ +++ VF   D T RV V  +GGWP W  DS +YFHR +DDGW
Sbjct: 213 ASFGEKGWTWSMVEKEISSDVYVFLTQDGTQRVKVVEQGGWPRWVDDSTLYFHRKSDDGW 272

Query: 248 WSIFRVEIPENLDSSVSPVPI-RVTPAGLHCFTPA-AMNDCNRVVVATRRPDSKFRHIEI 307
            S++R  +P+    +   V I RVTP GLH FTPA + N+ N + VATRRP S+ RH+E+
Sbjct: 273 ISVYRAILPKTGPVTTKSVTIQRVTPPGLHAFTPATSPNNNNFIAVATRRPGSEIRHVEL 332

Query: 308 FDSGSDEFVPITQKLNPNFHHYNPFVSPDSNYVGYHRFRGESSQGELTIPHLNSVISPIN 367
           FD   +EFV +T+ ++P  HH+NPF+SPDS+ VGYH  RG+++  +     L S+ +  N
Sbjct: 333 FDLKKNEFVELTRLVSPKSHHFNPFLSPDSSRVGYHSCRGDATGRKTPRNLLQSLKTTSN 392

Query: 368 ELRMIRLNGSFPTPSPDGDFIAFNPDFVGLKIVKSDGSKCWTVLKERTAFYNSWSPTEKN 427
           +L + R +G+FP+ SP+GD  AF   F G+ +V  DGS    +L +   F   W P    
Sbjct: 393 DLSLFRFDGAFPSISPEGDRFAF-VSFTGVFVVNPDGSGLRQLLPQ-MGFGTVWDPIRHG 452

Query: 428 VIYTSLGPIFGPPRATVQIARITINSDDLNGGDNEEVASEVKILTKDDSGNNAFPACSPD 487
           ++YTS GP   P ++ + I  I +        D    A+ VK LT     NNAFP  SPD
Sbjct: 453 IVYTSSGPALAPGKSQIDILAINV--------DAPSPATAVKKLTTTGE-NNAFPWPSPD 512

Query: 488 GKFLVFRSGRSGHKNLYIVDAVNGEFDGGIRRLTDGPWIDTMPSWSPTGDLIAFSSNMHN 547
           GK +VFRS RSG KNLYI+DA  GE  GG+ RLT+G W DT+ +WSP G+ I F+SN   
Sbjct: 513 GKRIVFRSARSGTKNLYIMDAEKGE-SGGLFRLTNGNWNDTIATWSPDGNWIVFASNREF 572

Query: 548 PKNSEVFSIYVIRPDGSGLRRVHVAGPDGCGDVDKERINHVCFSRDGQWLLFTSNLGGVT 607
           P  + + +IYV+ PDG+GLR++     +  G V      H  FS D + ++FT+   G++
Sbjct: 573 P-GTLLMNIYVVHPDGTGLRKL---AQNLTGLVSM----HPMFSPDSKRIVFTTIYAGIS 632

Query: 608 AEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNGYENGTPTWY 613
           AE +  P+   P  ++F V LDG+GL RLT N  E+G P W+
Sbjct: 633 AEQIGNPHFNVPSSEIFTVNLDGSGLTRLTHNSVEDGPPMWF 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022954120.10.0e+0087.38uncharacterized protein LOC111456480 [Cucurbita moschata][more]
KAG6575727.10.0e+0087.23hypothetical protein SDJN03_26366, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023547812.10.0e+0087.54uncharacterized protein LOC111806666 [Cucurbita pepo subsp. pepo][more]
KAG7014283.10.0e+0086.92tolB, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022991715.10.0e+0086.45uncharacterized protein LOC111488250 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q3A0976.3e-1627.34Tol-Pal system protein TolB OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 1... [more]
Q3APB51.4e-1527.15Protein TolB homolog OS=Chlorobium chlorochromatii (strain CaD3) OX=340177 GN=to... [more]
A8LHQ62.9e-1331.08Tol-Pal system protein TolB OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB ... [more]
Q0AC408.6e-1328.11Tol-Pal system protein TolB OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 /... [more]
Q167Z61.1e-1232.12Tol-Pal system protein TolB OS=Roseobacter denitrificans (strain ATCC 33942 / OC... [more]
Match NameE-valueIdentityDescription
A0A6J1GRK10.0e+0087.38uncharacterized protein LOC111456480 OS=Cucurbita moschata OX=3662 GN=LOC1114564... [more]
A0A6J1JTR30.0e+0086.45uncharacterized protein LOC111488250 OS=Cucurbita maxima OX=3661 GN=LOC111488250... [more]
A0A5A7URS20.0e+0083.49TolB protein-related isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CEJ80.0e+0083.02uncharacterized protein LOC103499982 OS=Cucumis melo OX=3656 GN=LOC103499982 PE=... [more]
A0A0A0K6G20.0e+0082.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G419590 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G01870.11.2e-25664.77tolB protein-related [more]
AT1G21680.12.8e-13641.90DPP6 N-terminal domain-like protein [more]
AT1G21670.15.1e-13040.97LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011659WD40-like Beta PropellerPFAMPF07676PD40coord: 439..468
e-value: 3.0E-4
score: 20.6
coord: 481..521
e-value: 3.2E-5
score: 23.7
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 98..329
e-value: 1.1E-10
score: 43.3
coord: 544..620
e-value: 1.0E-6
score: 30.3
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 330..543
e-value: 5.9E-31
score: 109.7
NoneNo IPR availablePANTHERPTHR32161DPP6 N-TERMINAL DOMAIN-LIKE PROTEINcoord: 5..645
NoneNo IPR availablePANTHERPTHR32161:SF9TOLB PROTEIN-LIKE PROTEINcoord: 5..645
NoneNo IPR availableSUPERFAMILY69304Tricorn protease N-terminal domaincoord: 4..281
NoneNo IPR availableSUPERFAMILY82171DPP6 N-terminal domain-likecoord: 340..616

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0002696.1Lag0002696.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis