Lag0002534 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002534
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	rapid alkalinization factor-like
Location	chr4: 43653558 .. 43653914 (+)
RNA-Seq Expression	Lag0002534
Synteny	Lag0002534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCAAGGTTTCTTTTCTCCTTCCATTTTGCTTCATCGTTGCAATCCTTACCGTCTCCTCCACTGCAGTATTGGCCACAAGCACCGAGAGTCCGAGCTGGGTCCTGGATGGAGCTCGAACTCGCTGTCACGGCTCCATGGCAAAGTGCATGATGGACGACATTGAATTCGAGATGGACTCAGAAATCAATAGGCGCATATTGGCAGATCTAAAGTACATAAGCTACGACGCACTCAAGGCCAACAAGGTGCCATGCTCACGCAGAGGAGCATCTTACTACAATTGCCAGCCAGGTGCCGAGGCAAACCCCTACAACCGTGGCTGCAATGCTATTGCTCGTTGCCGAAGCTAG

mRNA sequence

ATGGCGGCCAAGGTTTCTTTTCTCCTTCCATTTTGCTTCATCGTTGCAATCCTTACCGTCTCCTCCACTGCAGTATTGGCCACAAGCACCGAGAGTCCGAGCTGGGTCCTGGATGGAGCTCGAACTCGCTGTCACGGCTCCATGGCAAAGTGCATGATGGACGACATTGAATTCGAGATGGACTCAGAAATCAATAGGCGCATATTGGCAGATCTAAAGTACATAAGCTACGACGCACTCAAGGCCAACAAGGTGCCATGCTCACGCAGAGGAGCATCTTACTACAATTGCCAGCCAGGTGCCGAGGCAAACCCCTACAACCGTGGCTGCAATGCTATTGCTCGTTGCCGAAGCTAG

Coding sequence (CDS)

ATGGCGGCCAAGGTTTCTTTTCTCCTTCCATTTTGCTTCATCGTTGCAATCCTTACCGTCTCCTCCACTGCAGTATTGGCCACAAGCACCGAGAGTCCGAGCTGGGTCCTGGATGGAGCTCGAACTCGCTGTCACGGCTCCATGGCAAAGTGCATGATGGACGACATTGAATTCGAGATGGACTCAGAAATCAATAGGCGCATATTGGCAGATCTAAAGTACATAAGCTACGACGCACTCAAGGCCAACAAGGTGCCATGCTCACGCAGAGGAGCATCTTACTACAATTGCCAGCCAGGTGCCGAGGCAAACCCCTACAACCGTGGCTGCAATGCTATTGCTCGTTGCCGAAGCTAG

Protein sequence

MAAKVSFLLPFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMDDIEFEMDSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS

Homology

[Close]

BLAST of Lag0002534 vs. NCBI nr
Match: XP_038896330.1 (protein RALF-like 33 [Benincasa hispida])

HSP 1 Score: 208.4 bits (529), Expect = 3.4e-50
Identity = 102/118 (86.44%), Postives = 110/118 (93.22%), Query Frame = 0


Query: 1   MAAKVSFLLPFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMDDIEFEM 60
           MAAKVSFLLPF  + AILTVSSTAVL TST++PSWVLDGAR RCHGSMA+CMM DIEFEM
Sbjct: 1   MAAKVSFLLPFFLVAAILTVSSTAVLTTSTKTPSWVLDGARARCHGSMAECMMGDIEFEM 60

Query: 61  DSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           +S+INRRILADL YISYDALKAN VPCSRRGASYYNCQPGAEANPY+RGCNAI+RCRS
Sbjct: 61  NSDINRRILADLNYISYDALKANTVPCSRRGASYYNCQPGAEANPYDRGCNAISRCRS 118

[Close]

BLAST of Lag0002534 vs. NCBI nr
Match: XP_022153599.1 (protein RALF-like 33 [Momordica charantia])

HSP 1 Score: 205.7 bits (522), Expect = 2.2e-49
Identity = 101/118 (85.59%), Postives = 108/118 (91.53%), Query Frame = 0


Query: 1   MAAKVSFLLPFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMDDIEFEM 60
           MAAKVSFLLPFC I AILTVSSTAV A STESPSWVLDGAR RCHGSMA+CMMD+IE+EM
Sbjct: 1   MAAKVSFLLPFCLITAILTVSSTAVFAPSTESPSWVLDGARARCHGSMAECMMDEIEYEM 60

Query: 61  DSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           +SEINRRILA   YISY+ALKAN VPCSRRGASYYNCQPGAEANPY+RGC+AI RCRS
Sbjct: 61  NSEINRRILATSNYISYEALKANTVPCSRRGASYYNCQPGAEANPYDRGCSAITRCRS 118

[Close]

BLAST of Lag0002534 vs. NCBI nr
Match: KAG7014377.1 (Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 203.8 bits (517), Expect = 8.4e-49
Identity = 99/118 (83.90%), Postives = 107/118 (90.68%), Query Frame = 0


Query: 1   MAAKVSFLLPFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMDDIEFEM 60
           MAAK+SFLLPFCFI A LTV ST VLAT+T+ PSWVLD A   CHGSMA CMMDDIEF+M
Sbjct: 1   MAAKLSFLLPFCFIAAFLTVFSTVVLATTTKGPSWVLDRALATCHGSMADCMMDDIEFQM 60

Query: 61  DSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           DSEINRRILAD+ YISYDAL+ANKVPCSRRGASYYNCQPGAEANPY+RGC+AIARCRS
Sbjct: 61  DSEINRRILADVNYISYDALRANKVPCSRRGASYYNCQPGAEANPYDRGCSAIARCRS 118

[Close]

BLAST of Lag0002534 vs. NCBI nr
Match: XP_022953165.1 (rapid alkalinization factor-like [Cucurbita moschata])

HSP 1 Score: 203.0 bits (515), Expect = 1.4e-48
Identity = 99/118 (83.90%), Postives = 107/118 (90.68%), Query Frame = 0


Query: 1   MAAKVSFLLPFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMDDIEFEM 60
           MAAK+SFLLPFCFI A LTV STAV AT+T+ PSWVLD A   CHGSMA CMMDDIEF+M
Sbjct: 1   MAAKLSFLLPFCFIAAFLTVFSTAVSATTTKGPSWVLDRALATCHGSMADCMMDDIEFQM 60

Query: 61  DSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           DSEINRRILAD+ YISYDAL+ANKVPCSRRGASYYNCQPGAEANPY+RGC+AIARCRS
Sbjct: 61  DSEINRRILADVNYISYDALRANKVPCSRRGASYYNCQPGAEANPYDRGCSAIARCRS 118

[Close]

BLAST of Lag0002534 vs. NCBI nr
Match: XP_011659776.1 (rapid alkalinization factor [Cucumis sativus])

HSP 1 Score: 189.5 bits (480), Expect = 1.6e-44
Identity = 94/118 (79.66%), Postives = 103/118 (87.29%), Query Frame = 0


Query: 1   MAAKVSFLLPFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMDDIEFEM 60
           MA  VSFLLP   +VAILTVSSTA      ++PSWVLDGA  RCHGSMA+CMM+DIEF+M
Sbjct: 1   MATTVSFLLPIFLLVAILTVSSTA------KTPSWVLDGAHARCHGSMAECMMEDIEFQM 60

Query: 61  DSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           DSEINRRILADL YISYDALKAN VPCSR+GASYYNCQPGAEANPY+RGCNAI+RCRS
Sbjct: 61  DSEINRRILADLNYISYDALKANSVPCSRKGASYYNCQPGAEANPYDRGCNAISRCRS 112

[Close]

BLAST of Lag0002534 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 5.5e-27
Identity = 64/110 (58.18%), Postives = 79/110 (71.82%), Query Frame = 0


Query: 10  PFCFIVAILTVSSTAVLATSTESPSWVLDGARTRCHGSMAKCMMD--DIEFEMDSEINRR 69
           P   I+AILTV       TS  S  +V     ++C+G++A+C +   + EFEMDSEINRR
Sbjct: 8   PVAIIIAILTVHFLFAAVTSQSSGDFV--PIESKCNGTIAECSLSTAEEEFEMDSEINRR 67

Query: 70  ILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCR 118
           ILA  KYISY AL+ N VPCSRRGASYYNC+ GA+ANPY+RGC+AI RCR
Sbjct: 68  ILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of Lag0002534 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.1e-26
Identity = 60/111 (54.05%), Postives = 78/111 (70.27%), Query Frame = 0


Query: 9   LPFCFIVAILTVSSTAVLATSTESPSWVLDG-ARTRCHGSMAKCMMDDIEFEMDSEINRR 68
           L  C ++    +S  A  A  + +  WV+   +   C GS+ +C+ ++ EFE+DSE NRR
Sbjct: 7   LILCVLIGAFFISMAA--AGDSGAYDWVMPARSGGGCKGSIGECIAEEEEFELDSESNRR 66

Query: 69  ILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           ILA  KYISY AL+ N VPCSRRGASYYNC+PGA+ANPY+RGC+AI RCRS
Sbjct: 67  ILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of Lag0002534 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.8e-25
Identity = 63/109 (57.80%), Postives = 76/109 (69.72%), Query Frame = 0


Query: 14  IVAILTVSSTAV---LATSTESPSWVLDGAR-TRCHGSMAKCMMDDIEFEMDSEINRRIL 73
           I+ +  +SS  V    A      +W   G   + CHGS+A+C+  + E EMDSEINRRIL
Sbjct: 13  ILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAE-EEEMDSEINRRIL 72

Query: 74  ADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCRS 119
           A  KYISY +LK N VPCSRRGASYYNCQ GA+ANPY+RGC+ IARCRS
Sbjct: 73  ATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of Lag0002534 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.4e-24
Identity = 58/109 (53.21%), Postives = 79/109 (72.48%), Query Frame = 0


Query: 14  IVAILTVSSTAVLATST--ESPSWVLDGAR---TRCHGSMAKCMMDDIEFEMDSEINRRI 73
           ++AIL +  +AV +T    +S  +V  G+    + C GS+A+C+ ++ E E DS+I+RRI
Sbjct: 10  VIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRI 69

Query: 74  LADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIARCR 118
           LA  KYISY A++ N VPCSRRGASYYNCQ GA+ANPY+RGC+ I RCR
Sbjct: 70  LAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

[Close]

BLAST of Lag0002534 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.4e-22
Identity = 62/122 (50.82%), Postives = 80/122 (65.57%), Query Frame = 0


Query: 14  IVAILTVSSTAVLATSTESPSWVLD--GARTRCHGSMAKCMMD-------DI-------- 73
           I+ IL V + +V A S++S  +  D     T C G++A+C +        D+        
Sbjct: 16  ILLILAVHNWSV-AVSSQSTEFAGDFPPFETECRGTIAECSVSAALGDGGDLFYGGGEMG 75

Query: 74  -EFEMDSEINRRILADLKYISYDALKANKVPCSRRGASYYNCQPGAEANPYNRGCNAIAR 118
            EFEMDSEINRRILA  +YISY AL+ N +PCSRRGASYYNC+ GA+ANPY+RGC+AI R
Sbjct: 76  EEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITR 135

The following BLAST results are available for this feature:

BLAST of Lag0002534 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896330.1	3.4e-50	86.44	protein RALF-like 33 [Benincasa hispida]	[more]
XP_022153599.1	2.2e-49	85.59	protein RALF-like 33 [Momordica charantia]	[more]
KAG7014377.1	8.4e-49	83.90	Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022953165.1	1.4e-48	83.90	rapid alkalinization factor-like [Cucurbita moschata]	[more]
XP_011659776.1	1.6e-44	79.66	rapid alkalinization factor [Cucumis sativus]	[more]

BLAST of Lag0002534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9P8	5.5e-27	58.18	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]
Q945T0	6.1e-26	54.05	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q9SRY3	1.8e-25	57.80	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
Q9MA62	3.4e-24	53.21	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
Q9LUS7	1.4e-22	50.82	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 57..117 e-value: 1.0E-28 score: 100.1
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 7..118
None	No IPR available	PANTHER	PTHR33136:SF50	PROTEIN RALF-LIKE 33	coord: 7..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002534.1	Lag0002534.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane