Lag0002511 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002511
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cysteine protease XCP1-like
Location	chr4: 43469100 .. 43469684 (+)
RNA-Seq Expression	Lag0002511
Synteny	Lag0002511

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGACGGCTTATACTCGCTTGGCGTTCATCTTCTTCATCTTCTTCTTCCTCACCTGCCTTTTGCTTGATGCAGAATCGCCTCTACCGGAGGTTGCCTCGACACCGGGAGCCCATCACGGTTTCTTCTCTCTCTCTCTTTTTTTGACATTTTACAAAAGAATATTGGGAATTTTGTTATTTTCTTCTGCAGATTTATCCGTTTCATCCCTATGATCCCGCTGATGCTGGAAAGGTATGAATCATACTCAATTTTGCAATCTGGTTATGGTTGAATAATAATATAATTGTAAGATAAACTCAATCAGAATTTTATATTTTGTAGTTGGCTAAAGCACAGTCTTCGGAGCACTGGGAGCTGTTCCAAACGTGGATGAAGAATCACAAAAAGCGTTACAGGGGTGAGAAAGAGATGCTCTGTAGGTTTGAAATATTCACTAATTGTGTGAAGTTTATTGAGGAGAAGAACAAGGAGCTAGAAGGCTATCAACTGGGGTTGAATAATTACTCAGACTTGACAGATGAGGAGTTTCCTCGTGGGCTCGGCCGTGGAGTAGGAGTCCGTTTCGTCGGGCACAGATGA

mRNA sequence

ATGGCGGGACGGCTTATACTCGCTTGGCGTTCATCTTCTTCATCTTCTTCTTCCTCACCTGCCTTTTGCTTGATGCAGAATCGCCTCTACCGGAGGTTGCCTCGACACCGGGAGCCCATCACGATTTATCCGTTTCATCCCTATGATCCCGCTGATGCTGGAAAGTTGGCTAAAGCACAGTCTTCGGAGCACTGGGAGCTGTTCCAAACGTGGATGAAGAATCACAAAAAGCGTTACAGGGGTGAGAAAGAGATGCTCTGTAGGTTTGAAATATTCACTAATTGTGTGAAGTTTATTGAGGAGAAGAACAAGGAGCTAGAAGGCTATCAACTGGGGTTGAATAATTACTCAGACTTGACAGATGAGGAGTTTCCTCGTGGGCTCGGCCGTGGAGTAGGAGTCCGTTTCGTCGGGCACAGATGA

Coding sequence (CDS)

ATGGCGGGACGGCTTATACTCGCTTGGCGTTCATCTTCTTCATCTTCTTCTTCCTCACCTGCCTTTTGCTTGATGCAGAATCGCCTCTACCGGAGGTTGCCTCGACACCGGGAGCCCATCACGATTTATCCGTTTCATCCCTATGATCCCGCTGATGCTGGAAAGTTGGCTAAAGCACAGTCTTCGGAGCACTGGGAGCTGTTCCAAACGTGGATGAAGAATCACAAAAAGCGTTACAGGGGTGAGAAAGAGATGCTCTGTAGGTTTGAAATATTCACTAATTGTGTGAAGTTTATTGAGGAGAAGAACAAGGAGCTAGAAGGCTATCAACTGGGGTTGAATAATTACTCAGACTTGACAGATGAGGAGTTTCCTCGTGGGCTCGGCCGTGGAGTAGGAGTCCGTTTCGTCGGGCACAGATGA

Protein sequence

MAGRLILAWRSSSSSSSSSPAFCLMQNRLYRRLPRHREPITIYPFHPYDPADAGKLAKAQSSEHWELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEFPRGLGRGVGVRFVGHR

Homology

[Close]

BLAST of Lag0002511 vs. NCBI nr
Match: XP_038887495.1 (pro-cathepsin H-like [Benincasa hispida])

HSP 1 Score: 73.2 bits (178), Expect = 2.0e-09
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 0


Query: 62  SEHWELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTD 121
           SE WE F++WM  H K+Y  E+EML RF +F   +K IE+ NK   G   G N++SDLT 
Sbjct: 56  SEDWESFKSWMSMHNKKYGSEEEMLYRFGLFKKTLKRIEKLNKNYTGCTFGFNHFSDLTF 115

Query: 122 EEFPRGLGRGVGVRF 137
           +E P+G     G+ F
Sbjct: 116 DEVPKGYTSSNGMEF 130

[Close]

BLAST of Lag0002511 vs. NCBI nr
Match: KAE8650303.1 (hypothetical protein Csa_010836 [Cucumis sativus])

HSP 1 Score: 73.2 bits (178), Expect = 2.0e-09
Identity = 35/73 (47.95%), Postives = 46/73 (63.01%), Query Frame = 0


Query: 54  GKLAKAQSSEHWELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGL 113
           G L  A+ S+ W+ F +WM  HKK+Y  ++E L RF IF   +K I++ NKE  G   GL
Sbjct: 151 GLLEDAERSKDWKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGL 210

Query: 114 NNYSDLTDEEFPR 127
           N YSDLT+ EF R
Sbjct: 211 NQYSDLTNSEFNR 223

[Close]

BLAST of Lag0002511 vs. NCBI nr
Match: P05994.3 (RecName: Full=Papaya proteinase 4; AltName: Full=Glycyl endopeptidase; AltName: Full=Papaya peptidase B; AltName: Full=Papaya proteinase IV; Short=PPIV; Flags: Precursor [Carica papaya] >CAA54974.1 proteinase IV [Carica papaya])

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-08
Identity = 30/59 (50.85%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEF 125
           +LF +WM  H K Y+   E L RFEIF + +K+I+E+NK + GY LGLN +SDL+++EF
Sbjct: 46  QLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGYWLGLNEFSDLSNDEF 104

[Close]

BLAST of Lag0002511 vs. NCBI nr
Match: XP_038888276.1 (cysteine protease XCP1-like [Benincasa hispida])

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-08
Identity = 31/59 (52.54%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEF 125
           ELF++WMK H K+Y+  +E L RFEIF + +K I+E NK++  Y LGLN ++DL+ EEF
Sbjct: 46  ELFESWMKKHSKKYQSIEEKLHRFEIFIDNLKHIDETNKKVSSYWLGLNEFADLSHEEF 104

[Close]

BLAST of Lag0002511 vs. NCBI nr
Match: XP_021888999.1 (papaya proteinase 4 [Carica papaya])

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-08
Identity = 30/59 (50.85%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEF 125
           +LF +WM  H K Y+   E L RFEIF + +K+I+E+NK + GY LGLN +SDL+++EF
Sbjct: 46  QLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGYWLGLNEFSDLSNDEF 104

[Close]

BLAST of Lag0002511 vs. ExPASy Swiss-Prot
Match: P05994 (Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3)

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-11
Identity = 30/59 (50.85%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEF 125
           +LF +WM  H K Y+   E L RFEIF + +K+I+E+NK + GY LGLN +SDL+++EF
Sbjct: 46  QLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGYWLGLNEFSDLSNDEF 104

[Close]

BLAST of Lag0002511 vs. ExPASy Swiss-Prot
Match: O65493 (Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.3e-10
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEFP 125
           ELF++WM  H K Y+  +E + RFE+F   +  I+++N E+  Y LGLN ++DLT EEF 
Sbjct: 49  ELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFK 108

Query: 126 RGLGRGVGV 135
              GR +G+
Sbjct: 109 ---GRYLGL 114

[Close]

BLAST of Lag0002511 vs. ExPASy Swiss-Prot
Match: P10056 (Caricain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-09
Identity = 28/59 (47.46%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEF 125
           +LF +WM NH K Y    E L RFEIF + + +I+E NK+   Y LGLN ++DL+++EF
Sbjct: 46  QLFNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKKNNSYWLGLNEFADLSNDEF 104

[Close]

BLAST of Lag0002511 vs. ExPASy Swiss-Prot
Match: P00784 (Papain OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.1e-09
Identity = 26/59 (44.07%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEF 125
           +LF++WM  H K Y+   E + RFEIF + +K+I+E NK+   Y LGLN ++D++++EF
Sbjct: 46  QLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKKNNSYWLGLNVFADMSNDEF 104

[Close]

BLAST of Lag0002511 vs. ExPASy Swiss-Prot
Match: Q9LM66 (Cysteine protease XCP2 OS=Arabidopsis thaliana OX=3702 GN=XCP2 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 8.1e-09
Identity = 27/61 (44.26%), Postives = 41/61 (67.21%), Query Frame = 0


Query: 66  ELFQTWMKNHKKRYRGEKEMLCRFEIFTNCVKFIEEKNKELEGYQLGLNNYSDLTDEEFP 125
           ELF+ W+ N +K Y   +E   RFE+F + +K I+E NK+ + Y LGLN ++DL+ EEF 
Sbjct: 49  ELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFK 108

Query: 126 R 127
           +
Sbjct: 109 K 109

The following BLAST results are available for this feature:

BLAST of Lag0002511 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887495.1	2.0e-09	45.33	pro-cathepsin H-like [Benincasa hispida]	[more]
KAE8650303.1	2.0e-09	47.95	hypothetical protein Csa_010836 [Cucumis sativus]	[more]
P05994.3	1.3e-08	50.85	RecName: Full=Papaya proteinase 4; AltName: Full=Glycyl endopeptidase; AltName: ...	[more]
XP_038888276.1	1.3e-08	52.54	cysteine protease XCP1-like [Benincasa hispida]	[more]
XP_021888999.1	1.3e-08	50.85	papaya proteinase 4 [Carica papaya]	[more]

BLAST of Lag0002511 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05994	1.7e-11	50.85	Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3	[more]
O65493	4.3e-10	43.48	Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1	[more]
P10056	2.1e-09	47.46	Caricain OS=Carica papaya OX=3649 PE=1 SV=2	[more]
P00784	8.1e-09	44.07	Papain OS=Carica papaya OX=3649 PE=1 SV=1	[more]
Q9LM66	8.1e-09	44.26	Cysteine protease XCP2 OS=Arabidopsis thaliana OX=3702 GN=XCP2 PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 137..140
None	No IPR available	GENE3D	1.10.287.2250		coord: 50..130 e-value: 2.3E-20 score: 74.4
None	No IPR available	PANTHER	PTHR12411:SF745	CYSTEINE PROTEASE RDL2-RELATED	coord: 55..124
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 55..124
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 68..124 e-value: 3.6E-18 score: 76.4
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 68..124 e-value: 3.5E-15 score: 56.2
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 62..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002511.1	Lag0002511.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008233	peptidase activity