Homology
BLAST of Lag0002435 vs. NCBI nr
Match:
XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])
HSP 1 Score: 1550.8 bits (4014), Expect = 0.0e+00
Identity = 756/883 (85.62%), Postives = 806/883 (91.28%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS VIQPFCVYN GV ESFKLAV DHHT+G KIKY SGKMDEARMLA G S+VG
Sbjct: 1 MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
KSSHRNLSK ATSGI V KSGQRFGT GKSKEH KVATYLFRTEFGDLVNV+VGKTGS
Sbjct: 61 TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
GF V +E+SS+QLASI+ +LSWG+YRSDSAI+TPNFQSSLP+ET+G TETPFV S G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSFLLKYP+GVD GSSEIRSHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLD YINRSG+IWHASFE ASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG------LGQICKVPAFDW 360
YGYRCKGSKFHEN+D VEV+R+++DPYAKILT S KT GQG LGQICKVPAFDW
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLPKFLGQICKVPAFDW 360
Query: 361 DGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLLE 420
DGEVCPNLPMEKLF YRLNVKRFTVDKSSQL DIAGTFSGLTKK LH KNLGVNAVLLE
Sbjct: 361 DGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLE 420
Query: 421 PIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTHT 480
PIF FDEKEG YFP HFFSP N +GPSG SISAINS+K MVK+LHANG+EVILEVVFTHT
Sbjct: 421 PIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTHT 480
Query: 481 AEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFIN 540
A DGAL+GIDDSSYYF NRVAN+EGKSAL+CNYPIVQQLLLDSLRYWV EFHIDGFCFIN
Sbjct: 481 AMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFIN 540
Query: 541 ASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVN 600
ASSLLRGHHGE LSR P VEAIAFDPLLSKTK++ADFWDPQE V+ ET PHWKRWAEVN
Sbjct: 541 ASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEVN 600
Query: 601 SKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSS 660
SKFCNDVR FF+GEGL+SSLATRLCGSGD+FS+GRGPAFSFNFI RN GLPLVDLVSFS+
Sbjct: 601 SKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFSN 660
Query: 661 SKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 720
SKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFV+FVSLGVPVLNMGDECGQS+GGSV
Sbjct: 661 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGSV 720
Query: 721 AFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSPP 780
AFNDR++F+W++LKT FGTQTTQFITFLSSFR +RFDLFQSRNFLKGENIDW+ NQSPP
Sbjct: 721 AFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPP 780
Query: 781 QWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWFR 840
QWEDPSCKFLAVMLRADKEENGSI EN +PRSDIFMAFNASDQSE+VALPEPSEGTSWFR
Sbjct: 781 QWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWFR 840
Query: 841 LIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
L+DTALSFPGFFSSDGE VP TGSVTYEMQAHSCALFEAKS N
Sbjct: 841 LVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFN 883
BLAST of Lag0002435 vs. NCBI nr
Match:
XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])
HSP 1 Score: 1512.7 bits (3915), Expect = 0.0e+00
Identity = 739/882 (83.79%), Postives = 798/882 (90.48%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATF SS V+QP+ V+N GV E+F+LA SDHHTHGH IK+QSGK DE RMLA G S+ GF
Sbjct: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
KSS NLSKA A+ GISVEK+G RFG SGKSKE KVATYLFRTE GDLVNV V KTGS
Sbjct: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
GF V IE+SS+QLA+I+ PMLSWG+YRSDSA++TPN QSSLP++T+GT ETPFVN SEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKHVPFYLSFLLK+P+GVD GSSEIRSHRKT+FC+PVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGS+NFSIFSS+AESVVLCLY+DSTSEKPV+ELDLD YINRSGNIWH SFE SNFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY CKGSK H+ +D VE+SR+L+DPYAK+LT S+ KT G G LGQICKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNL MEKLF YRLNVKRFTVDKSSQL+TDIAGTF+GLTKK LH KNLGVNAVLL
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIFPFDEKEGPYFPSHFFSPTNI+GPSG SISAINS+KEMVKELHANGIEV+LEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
TAEDG L+GIDDSSYYF NRVAN+EGK+AL+CNYP+VQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ES++ ETR PHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
N KFC+DVR FF+GEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
SSKLASELSWNCGEEGPT+N EVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
+AF DR+ FDW++LKTGFGTQTTQFITF SSFR +RFDLFQSRNFLKGENIDWYG N SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWEDPSCKFLAVMLRADKEENG ENPEP+SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKS 875
RLIDTALSFPGFFSSDGE VP SVTYEMQAHSCALFEAKS
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 888
BLAST of Lag0002435 vs. NCBI nr
Match:
KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1505.7 bits (3897), Expect = 0.0e+00
Identity = 736/884 (83.26%), Postives = 797/884 (90.16%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS +IQPFC YN G ESFKLAV+D+HT+G K+ YQSGK+DEARM A G SKVGF
Sbjct: 24 MATFLSS-LIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
KS HRN+ KA A SGISV KSGQ+FGT GK++E KVATYLFRTEFGDLV +VGK GS
Sbjct: 84 TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
GF V IE+SSL+LASID +LSWG+YRS+SAI+TPNFQS LP ET+G TETPFV SEG
Sbjct: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSFLLKYP+GVD GSSEI+SHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLDPYINRSGNIWH SFEGASNFVS
Sbjct: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGYRC+GSKFHEN+D+ EVSR+L+DPYAKILT STPKT GQG LGQIC VPAF
Sbjct: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLF+YRLNVK FTVDKSSQL DIAGTFSGLT+K LH K LGVNAVLL
Sbjct: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIFPFDEK+GPYFP HFFSPTNI+ PSG+SIS++NS+KEMVKELHANG+EVILEVVFTH
Sbjct: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
TA DGAL+GIDDS YYF NRVAN EGKSAL+CN+PIVQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIA+FWDPQE V ETR PHWKRWAE+
Sbjct: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKF ND++ FF+GEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
SSKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAF DRK+F+W+ LKT FGTQTT+FI F SSFR +RFDLFQSR+FLKGENIDW+ NQSP
Sbjct: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
P+WEDPSCKFLAVMLRADKEE+GSI ENP+P+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
RL+DTALSFPGFFSSDGEPVP T VTYEMQAHSC LFEAKS N
Sbjct: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFN 906
BLAST of Lag0002435 vs. NCBI nr
Match:
XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])
HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 735/884 (83.14%), Postives = 796/884 (90.05%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS VIQPFCV+NCGV ES KLAVSDH T+G K KYQSGKMDEARMLA G SKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
+KSSHRNLSKA TSGI V KSGQR G GKSKE KVATYLFRTEFGDLVNV+VGKTGS
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
F V IE+SS+QL S D +LSW +YRSDSA +T NFQSS P+ T G TETPFV SEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSF+LK+P+GVD GSSEIRSHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKP+LELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY+CKG K HEN+D +EVSR+++DPYAKIL S PK+ QG LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNVKRFT+DKSSQL DIAGTFSGLTKK LHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN +GPSG+SISAINS+KEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
T+ DGAL+GIDDSSYYFANRVAN+E +SAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE V+ ETR PHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTN +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAFNDR++F+W++LKT FGTQTTQFI FLSSFR +RFDLFQSRNFLKGENIDW+ NQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAVMLRADKEEN SI ENP+ RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
R++DTAL FPGFFSSDGE VP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Lag0002435 vs. NCBI nr
Match:
KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])
HSP 1 Score: 1503.0 bits (3890), Expect = 0.0e+00
Identity = 734/884 (83.03%), Postives = 795/884 (89.93%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS VIQPFCV+NCGV ES KLAVSDH T+G K KYQSGKMDEARMLA G SKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
+KSSHRNLSKA TSGISV KSGQR G GKSKE KVATYLFRTEFGDLVNV+VGKTGS
Sbjct: 61 VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
F V IE+SS+QL S D +LSW +YRSDSA +T NFQSS P+ T G TETPFV SEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSF+LK+P+GVD GSSEIRSHRKT+F +PVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKP+LELDLDPYINRSGNIWHASFEG SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY+CKG K HEN+D +EVSR+++DPYAKIL S PK+ QG LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNVKRFT+DKSSQL DIAGTFSGLTKK LHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN +GPSG+SISAINS+KEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
T+ DGAL+GIDDSSYYFANRVAN+E +SAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE V+ ETR PHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTN +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAFNDR++F+W++LKT FGTQTTQFI FLSSFR +RFDLFQSRNFLKGENIDW+ NQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAVMLRADKEEN SI ENP+ RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
R++DTAL FPGFFSSDGE VP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Lag0002435 vs. ExPASy Swiss-Prot
Match:
Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)
HSP 1 Score: 955.7 bits (2469), Expect = 3.7e-277
Identity = 480/869 (55.24%), Postives = 625/869 (71.92%), Query Frame = 0
Query: 14 CVYNCGVAESFKLAVSDHHT-HGHKIKYQSGKMDEARMLALGSKVGFIKSSHRNLSKANA 73
C N G+ ++K + T +KIK S + R +G V S++ SK A
Sbjct: 13 CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQ-FSKICA 72
Query: 74 TSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGSGFAVYIEMSSLQL 133
S S+E ++ + KV +Y FRT+ G LV V V K +++ + +SSL+L
Sbjct: 73 -SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLEL 132
Query: 134 ASIDGIPM-LSWGIYRSDSAIITP-NFQSSLPNETVGTTETPFVNASEGKFVVELEFDAK 193
+ D + + WG+YRSDS+ P +F++S + TTET FV +S + ++ LEFD K
Sbjct: 133 SGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGK 192
Query: 194 HVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSIS-GDGSINFSI 253
PFYLSF LK G DP E+ +HR T+FCIPVGF G+P PLGLS D S NFS
Sbjct: 193 ESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSF 252
Query: 254 FSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSYGYRCKGSKF 313
FS ++ +VVLCLYDDST++KP LELDLDPY+NR+G++WHAS + +FV YGYRCK +
Sbjct: 253 FSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAH 312
Query: 314 HENKDKVEVSRVLLDPYAKILTVSTPKTCGQGLGQICKVPAFDWDGEVCPNLPMEKLFVY 373
+ VE ++LDPYA ++ S + + LG + K P+FDW +V PN+P+EKL VY
Sbjct: 313 SKEDVDVEGEPIVLDPYATVVGKSVSQ---KYLGSLSKSPSFDWGEDVSPNIPLEKLLVY 372
Query: 374 RLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFPSH 433
RLNVK FT +SS+L +++AGTFSG+ +K HLK LG NAVLLEPIF F E++GPYFP H
Sbjct: 373 RLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH 432
Query: 434 FFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTHTAEDGALKGIDDSSYYF 493
FFSP +I+GPS S SA+NS+K MVK+LH+ GIEV+LEVVFTHTA+ GAL+GIDDSSYY+
Sbjct: 433 FFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYY 492
Query: 494 ANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRP 553
R +++ KS L+CNYP+VQQL+L+SLRYWV EFH+DGFCFINASSLLRG HGE LSRP
Sbjct: 493 KGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 552
Query: 554 PLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGEGL 613
PLVEAIAFDPLL++TK+IAD WDP E + E R PHWKRWAE+N+++C +VR+F +G G+
Sbjct: 553 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGV 612
Query: 614 VSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELSWNCGEEGP 673
+S LATR+CGSGD+F+DGRGPAFSFN+I+RN+GL LVD+VSFS +LASELSWNCGEEG
Sbjct: 613 LSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGA 672
Query: 674 TNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRKAFDWNVLKTG 733
TN S VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS RK FDWN+L +
Sbjct: 673 TNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASA 732
Query: 734 FGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLR- 793
FGTQ TQFI+F++S R +R D+FQ R+FLK ENI WY +Q+ P+WEDP+ KFLA+ ++
Sbjct: 733 FGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKS 792
Query: 794 -ADKEENGSIIENPEPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGFFS 853
+++EE S+ E EP+S D+F+ FNASD ESV LP +G+ W RL+DTAL FPGFFS
Sbjct: 793 ESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFS 852
Query: 854 SDGEPV----PKTGSVTYEMQAHSCALFE 872
+GE V P V YEM+ +SC LFE
Sbjct: 853 VEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Lag0002435 vs. ExPASy Swiss-Prot
Match:
Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)
HSP 1 Score: 765.0 bits (1974), Expect = 9.3e-220
Identity = 377/722 (52.22%), Postives = 500/722 (69.25%), Query Frame = 0
Query: 176 ASEGKFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPL 235
A EL FD PFYLSFLL D +EIR+HRKT+F +PVG G G P+PL
Sbjct: 99 AGGASLAAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPL 158
Query: 236 GLSISGDGSINFSIFSSTAESVVLCLYDDST----SEKPVLELDLDPYINRSGNIWHASF 295
G+SISGDG++NF+++S A +V L LY + ++P LE+DLDPYI+R+GN+WH S
Sbjct: 159 GMSISGDGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSL 218
Query: 296 EGASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKIL--TVSTPKTCGQGL------- 355
+VSY + C G + R LLDPYAK++ VS+ +G+
Sbjct: 219 ASVDGYVSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRC 278
Query: 356 -GQICKVPAFDWDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLH 415
+ P+++W + P LP+EKL VYR NV FT D+SS L D AGTF+GL+ K H
Sbjct: 279 FASLAIAPSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEH 338
Query: 416 LKNLGVNAVLLEPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANG 475
++LGVNA+LLEP+FPF + +GPYFP HFFSP N++ G S+SAI S+K+MV+ +H NG
Sbjct: 339 FRSLGVNAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNG 398
Query: 476 IEVILEVVFTHTAEDG------ALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLD 535
IEV+LEVVFTHTAE +++GID+SSYY AN +A + S L+CN+P+ Q+L+LD
Sbjct: 399 IEVLLEVVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILD 458
Query: 536 SLRYWVAEFHIDGFCFINASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQE 595
SLR+WV +FH+DGFCFINA L+RG GE LSRPPL+EAI FDP+LS TKIIAD W P +
Sbjct: 459 SLRHWVLDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLD 518
Query: 596 SVTNETRLPHWKRWAEVNSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFN 655
+ PHWKRWAEVN++F DVR F K E L+S LATRLCGSGD+FS RGPAFSFN
Sbjct: 519 ISNVQFPFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFN 578
Query: 656 FIARNAGLPLVDLVSFSSSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLG 715
++RN+GL LVDLVSFS+ L SE SWNCGEEGP+ NS VL+ RL+QIRNF+F+LFVSLG
Sbjct: 579 HVSRNSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLG 638
Query: 716 VPVLNMGDECGQSSGGSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSR 775
VPVLNMGDECG S+ GSV++ DR +W +KT F + T FI+FL++ R +R D+FQ R
Sbjct: 639 VPVLNMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRR 698
Query: 776 NFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASD 835
FLK ENI WYG++ P W+DP+ FL + + A+ +E + R D+++ FNA++
Sbjct: 699 EFLKLENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANE 758
Query: 836 QSESVALPEPSEGTSWFRLIDTALSFPGFFSSDGEPVPK--TGSVTYEMQAHSCALFEAK 876
+S S ALP +EG+ W RL+DT+L+FPGFF+++ P + G +Y ++AH+C LFE+K
Sbjct: 759 ESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESK 798
BLAST of Lag0002435 vs. ExPASy Swiss-Prot
Match:
B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)
HSP 1 Score: 374.0 bits (959), Expect = 4.6e-102
Identity = 257/712 (36.10%), Postives = 363/712 (50.98%), Query Frame = 0
Query: 223 IPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----DDSTSEKPVLELDLD 282
+P + G PLG+S +G +NF++FS A SV+LCL + V+E LD
Sbjct: 96 MPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLD 155
Query: 283 PYINRSGNIWHASFEG-ASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILT----- 342
N++G+IWH EG ++ V YGYR G + + + + S VLLDPYAK+++
Sbjct: 156 QQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRKYF 215
Query: 343 -VSTPKTCGQGLGQICKVPAFDW-DGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIA 402
V+ K+ FDW D PNLP L +Y +NV+ FT D+SS L +
Sbjct: 216 GVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDSTSR 275
Query: 403 GTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFPS--------------HFFSPTN 462
G++ GL K HL LGVNAV L P+F +DE E +P+ +FF+P +
Sbjct: 276 GSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAPMS 335
Query: 463 IFGPSGSS-ISAINSLKEMVKELHANGIEVILEVVFTHT--AEDG-----ALKGIDDSSY 522
+ +G ++A LK+MVKELH GIEVIL+VV+ HT A+D + +GID+ Y
Sbjct: 336 RYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVY 395
Query: 523 YFANRVANVE------GKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGH 582
Y + N E + L+CN+P+V++L+LDSLR+WV E+HIDGF F AS L RG
Sbjct: 396 YMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCRGP 455
Query: 583 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVR 642
G L PPL++ IA D +LS+ KIIA+ WD + R P+W RWAE N K+ +D+R
Sbjct: 456 DGCPLDAPPLIKEIAKDAVLSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGKYRDDLR 515
Query: 643 SFFKGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNAGLPLVDLVSFS------- 702
F KG+ G+ ATR+ GS D++ + R P NF+ + G L DLVS++
Sbjct: 516 RFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDAN 575
Query: 703 ----SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-- 762
SWNCG EG TN+ VL R +Q++NF L +S G P++ MGDE G
Sbjct: 576 GEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHT 635
Query: 763 -----QSSGGSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGE 822
S G N+ F W L+ +F + + FR L + R FL
Sbjct: 636 RYGNNNSYGHDTCINN---FQWEQLEQRRDGH-FRFFSEMIKFRHSNPILRRDR-FLNKN 695
Query: 823 NIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVA 874
++ W+ WE+ KFLA + D G DI++AFNA D
Sbjct: 696 DVTWH-----EDCWENQESKFLAFTVH-DHNSGG----------DIYLAFNAHDYFVDAV 755
BLAST of Lag0002435 vs. ExPASy Swiss-Prot
Match:
Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)
HSP 1 Score: 373.2 bits (957), Expect = 7.8e-102
Identity = 248/679 (36.52%), Postives = 347/679 (51.10%), Query Frame = 0
Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----DDSTSEKPVLELDLDPYINRSG 289
G SPLG+S D INF++FS A SV LCL D T + ++EL LDP +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 290 NIWHASFEGAS-NFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILT-VSTPKTCGQGL 349
+ WH E N V YGYR G + + + S +LLDPYAK++ S+ Q
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKF 207
Query: 350 GQI-----CKVPAFDW-DGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLT 409
Q + FDW D PN+P + L +Y +NV+ FT D+SS + I G++ G
Sbjct: 208 AQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFI 267
Query: 410 KKFLHLKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIFGP-SG 469
+K HL++LG+NAV L P+F FDE E Y +FF+P + + G
Sbjct: 268 EKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEG 327
Query: 470 SSISAINSLKEMVKELHANGIEVILEVVFTHTAE-------DGALKGIDDSSYYFA---N 529
I A KEMVK LH+ GIEVIL+VV+ HT E + +GID+ YY N
Sbjct: 328 DPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNN 387
Query: 530 RVANVEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRPP 589
++ N G + L+CN+P+V +L+LDSLR+WV E+H+DGF F AS L R G LS PP
Sbjct: 388 QLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPP 447
Query: 590 LVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE-GL 649
L+ AIA D +LS+ KIIA+ WD + + P+W RWAE N + +DVR F KG+ G+
Sbjct: 448 LIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGM 507
Query: 650 VSSLATRLCGSGDIFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSKLA 709
S ATR+ GS D++ + R P NF+ + G L DLVS++ +
Sbjct: 508 KGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCN 567
Query: 710 SELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-------QSSG 769
SWNCG EG T ++ + R +Q++NF L +S G P++ MGDE G S G
Sbjct: 568 DNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYG 627
Query: 770 GSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQ 829
+ N+ F W L +F + + FR + + NFL I W+ N
Sbjct: 628 HDTSLNN---FQWKELDAK-KQNHFRFFSEVIKFRHSH-HVLKHENFLTQGEITWHEDN- 687
Query: 830 SPPQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTS 850
W++ KFLA L + DI++AFNA D +P+P G
Sbjct: 688 ----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQ 743
BLAST of Lag0002435 vs. ExPASy Swiss-Prot
Match:
D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 348.6 bits (893), Expect = 2.1e-94
Identity = 235/693 (33.91%), Postives = 342/693 (49.35%), Query Frame = 0
Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--DDSTSEKPVLELDLDPYINRSGNIW 289
G P+PLG + + DG +NF+++S+ A + LCL+ DD +++ E+ LDP NR+GN+W
Sbjct: 99 GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158
Query: 290 HASFEGASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQGLGQIC- 349
H EG + + YGYR G +VS V++DPYAK + +S + G G C
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAV-ISRGEYGVPGPGGDCW 218
Query: 350 -----KVP----AFDWDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTK 409
+P FDW G++ P + L +Y ++++ FT K S + + GT+ G
Sbjct: 219 PQMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFT--KHSSSNVEHPGTYIGAIS 278
Query: 410 KFLHLKNLGVNAVLLEPIFPFDEKE----------GPYFPSHFFSPTNIFGPSG---SSI 469
K +LK LGVN V L P F+E E Y +FFSP + G
Sbjct: 279 KLDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGR 338
Query: 470 SAINSLKEMVKELHANGIEVILEVVFTHTAEDG------ALKGIDDSSYYF---ANRVAN 529
AIN K V+E H GIEVI++VVF HTAE + +GID+S+YY N
Sbjct: 339 DAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYN 398
Query: 530 VEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRG-------------- 589
G + +CN+P+V++ ++D LRYWV E H+DGF F AS + RG
Sbjct: 399 YSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPV 458
Query: 590 -----HHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSK 649
G L+ PPL++ I+ DP+L K+IA+ WD + + PHWK W+E N K
Sbjct: 459 EGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWD-AGGLYQVGQFPHWKIWSEWNGK 518
Query: 650 FCNDVRSFFKG-EGLVSSLATRLCGSGDIF-SDGRGPAFSFNFIARNAGLPLVDLVSFSS 709
+ + VR F KG +G A LCGS ++ + GR P S NF+ + G L DLV+++
Sbjct: 519 YRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNK 578
Query: 710 SKLAS-----------ELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMG 769
+S LSWNCGEEG V R +Q+RNF L VS GVP+ MG
Sbjct: 579 KYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMG 638
Query: 770 DECGQSSGG--SVAFNDR--KAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFL 829
DE G + GG + +D F W+ K + +F + ++ FR K+ + +F
Sbjct: 639 DEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFP 698
Query: 830 KGENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSE 852
+ + W+G P W + S +F+A + E + +I++AFNAS
Sbjct: 699 TAQRLHWHGHQPGKPDWSETS-RFVAFSTK------------DETKGEIYVAFNASHLPA 758
BLAST of Lag0002435 vs. ExPASy TrEMBL
Match:
A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)
HSP 1 Score: 1512.7 bits (3915), Expect = 0.0e+00
Identity = 739/882 (83.79%), Postives = 798/882 (90.48%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATF SS V+QP+ V+N GV E+F+LA SDHHTHGH IK+QSGK DE RMLA G S+ GF
Sbjct: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
KSS NLSKA A+ GISVEK+G RFG SGKSKE KVATYLFRTE GDLVNV V KTGS
Sbjct: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
GF V IE+SS+QLA+I+ PMLSWG+YRSDSA++TPN QSSLP++T+GT ETPFVN SEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKHVPFYLSFLLK+P+GVD GSSEIRSHRKT+FC+PVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGS+NFSIFSS+AESVVLCLY+DSTSEKPV+ELDLD YINRSGNIWH SFE SNFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY CKGSK H+ +D VE+SR+L+DPYAK+LT S+ KT G G LGQICKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNL MEKLF YRLNVKRFTVDKSSQL+TDIAGTF+GLTKK LH KNLGVNAVLL
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIFPFDEKEGPYFPSHFFSPTNI+GPSG SISAINS+KEMVKELHANGIEV+LEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
TAEDG L+GIDDSSYYF NRVAN+EGK+AL+CNYP+VQQLLLDSLRYWV EFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ES++ ETR PHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
N KFC+DVR FF+GEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
SSKLASELSWNCGEEGPT+N EVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
+AF DR+ FDW++LKTGFGTQTTQFITF SSFR +RFDLFQSRNFLKGENIDWYG N SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWEDPSCKFLAVMLRADKEENG ENPEP+SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKS 875
RLIDTALSFPGFFSSDGE VP SVTYEMQAHSCALFEAKS
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 888
BLAST of Lag0002435 vs. ExPASy TrEMBL
Match:
A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)
HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 735/884 (83.14%), Postives = 796/884 (90.05%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS VIQPFCV+NCGV ES KLAVSDH T+G K KYQSGKMDEARMLA G SKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
+KSSHRNLSKA TSGI V KSGQR G GKSKE KVATYLFRTEFGDLVNV+VGKTGS
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
F V IE+SS+QL S D +LSW +YRSDSA +T NFQSS P+ T G TETPFV SEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSF+LK+P+GVD GSSEIRSHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKP+LELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY+CKG K HEN+D +EVSR+++DPYAKIL S PK+ QG LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNVKRFT+DKSSQL DIAGTFSGLTKK LHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN +GPSG+SISAINS+KEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
T+ DGAL+GIDDSSYYFANRVAN+E +SAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE V+ ETR PHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTN +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAFNDR++F+W++LKT FGTQTTQFI FLSSFR +RFDLFQSRNFLKGENIDW+ NQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAVMLRADKEEN SI ENP+ RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
R++DTAL FPGFFSSDGE VP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Lag0002435 vs. ExPASy TrEMBL
Match:
A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)
HSP 1 Score: 1503.0 bits (3890), Expect = 0.0e+00
Identity = 734/884 (83.03%), Postives = 795/884 (89.93%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS VIQPFCV+NCGV ES KLAVSDH T+G K KYQSGKMDEARMLA G SKVG
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
+KSSHRNLSKA TSGISV KSGQR G GKSKE KVATYLFRTEFGDLVNV+VGKTGS
Sbjct: 61 VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
F V IE+SS+QL S D +LSW +YRSDSA +T NFQSS P+ T G TETPFV SEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSF+LK+P+GVD GSSEIRSHRKT+F +PVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKP+LELDLDPYINRSGNIWHASFEG SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY+CKG K HEN+D +EVSR+++DPYAKIL S PK+ QG LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNVKRFT+DKSSQL DIAGTFSGLTKK LHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN +GPSG+SISAINS+KEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
T+ DGAL+GIDDSSYYFANRVAN+E +SAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE V+ ETR PHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTN +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAFNDR++F+W++LKT FGTQTTQFI FLSSFR +RFDLFQSRNFLKGENIDW+ NQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAVMLRADKEEN SI ENP+ RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
R++DTAL FPGFFSSDGE VP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of Lag0002435 vs. ExPASy TrEMBL
Match:
A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)
HSP 1 Score: 1500.7 bits (3884), Expect = 0.0e+00
Identity = 736/884 (83.26%), Postives = 794/884 (89.82%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS +IQPFC YN G ESFKLAVSD+HT+G K+ YQSGK+DEARM A G SKVGF
Sbjct: 1 MATFLSS-LIQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
KS HRN+ +A A S ISV KSGQ+FGT GK++E KVATYLFRTEFGDLV +VGK GS
Sbjct: 61 TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
GF IE+SSL+LASID +LSWG+YRS+SAI+TPNFQS LP ET+G TETPFV SEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSFLLKYP+GVD GSSEI+SHRKT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGSINFSIFSSTAESVVLCLY+DSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGYRC+GSKFHEN+D+ EVSR+L+DPYAKILT S PKT GQG LGQIC VPAF
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLF+YRLNVK FTVDKSSQL D AGTFSGLT+K LH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIFPFDEK+GPYFP HFFSPTNI+ PSG+SIS++NS+KEMVKELHANG+EVILEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
TA DGAL+GIDDS YYF NRVAN EGKSAL+CN+PIVQQLLLDSLR+WV EFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQESV ETR PHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKF ND++ FF+GEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
SSKLASELSWNCGEEGPT+N EVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAF DRK+F+W+ LKT FGTQTTQFITFLSSFR +RFDLFQSRNFLK ENIDW+ NQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
P WEDPSCKFLAVMLRADKEE+GSI ENP+P+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
RL+DTALSFPGFFSSDGEPVP T VTY MQAHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 883
BLAST of Lag0002435 vs. ExPASy TrEMBL
Match:
A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)
HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 724/884 (81.90%), Postives = 794/884 (89.82%), Query Frame = 0
Query: 1 MATFLSSPVIQPFCVYNCGVAESFKLAVSDHHTHGHKIKYQSGKMDEARMLALG-SKVGF 60
MATFLSS VIQPFCVYNCGV ES KLA SDH T+G K KYQ GKMDEARMLA G +KVG
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 61 IKSSHRNLSKANATSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGS 120
+KSSHRNLSK A SGISV KSGQR G GKSKE +VATYLFRTEFGDLVNV+VGK GS
Sbjct: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
Query: 121 GFAVYIEMSSLQLASIDGIPMLSWGIYRSDSAIITPNFQSSLPNETVGTTETPFVNASEG 180
F V IE+ S+QL SID +LSWG+YRSDSA++TPNF+SS P+ET G TETPFV SEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
Query: 181 KFVVELEFDAKHVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSI 240
KF VELEFDAKH PFYLSF+LKYP+GVD GSSEIRSH+KT+F +PVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSINFSIFSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
SGDGS+NFSIFSS+AES+VLCLY+DSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
Query: 301 YGYRCKGSKFHENKDKVEVSRVLLDPYAKILTVSTPKTCGQG-------LGQICKVPAFD 360
YGY+CKGSK HEN+D +EVSR+++DPYAKIL S PK+ GQG LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
Query: 361 WDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQL DIAGTFSGLTKK LH KNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTH 480
EPIF FDEKEGPYFP HFFSPTN +GPSG+SISAINS+KEMVKELHANG+EVILEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
Query: 481 TAEDGALKGIDDSSYYFANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFI 540
T+ +GAL+GIDDSSYYF NRVAN+E KSAL+CNYPIVQQLLLDSLRYWV EFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEV 600
NAS LLRGHHGELLSRPP VEAIAFDPLLSKTK++ADFWDPQE + ETR PHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
Query: 601 NSKFCNDVRSFFKGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
NSKFC+D+R FF+GEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 SSKLASELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPT+N +VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSP 780
VAFND+++F+W++LKT FGTQTTQFI FLSSFR +RFDLFQ+RNFLKGENIDW+ NQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
PQWED SCKFLAVMLRADKEEN SI ENP+ RS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
Query: 841 RLIDTALSFPGFFSSDGEPVPKTGSVTYEMQAHSCALFEAKSSN 877
R++DTAL FPGFFSSDGE VP TGSVTYE+QAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884
BLAST of Lag0002435 vs. TAIR 10
Match:
AT1G03310.2 (debranching enzyme 1 )
HSP 1 Score: 955.7 bits (2469), Expect = 2.6e-278
Identity = 480/869 (55.24%), Postives = 625/869 (71.92%), Query Frame = 0
Query: 14 CVYNCGVAESFKLAVSDHHT-HGHKIKYQSGKMDEARMLALGSKVGFIKSSHRNLSKANA 73
C N G+ ++K + T +KIK S + R +G V S++ SK A
Sbjct: 13 CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQ-FSKICA 72
Query: 74 TSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGSGFAVYIEMSSLQL 133
S S+E ++ + KV +Y FRT+ G LV V V K +++ + +SSL+L
Sbjct: 73 -SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLEL 132
Query: 134 ASIDGIPM-LSWGIYRSDSAIITP-NFQSSLPNETVGTTETPFVNASEGKFVVELEFDAK 193
+ D + + WG+YRSDS+ P +F++S + TTET FV +S + ++ LEFD K
Sbjct: 133 SGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGK 192
Query: 194 HVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSIS-GDGSINFSI 253
PFYLSF LK G DP E+ +HR T+FCIPVGF G+P PLGLS D S NFS
Sbjct: 193 ESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSF 252
Query: 254 FSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSYGYRCKGSKF 313
FS ++ +VVLCLYDDST++KP LELDLDPY+NR+G++WHAS + +FV YGYRCK +
Sbjct: 253 FSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAH 312
Query: 314 HENKDKVEVSRVLLDPYAKILTVSTPKTCGQGLGQICKVPAFDWDGEVCPNLPMEKLFVY 373
+ VE ++LDPYA ++ S + + LG + K P+FDW +V PN+P+EKL VY
Sbjct: 313 SKEDVDVEGEPIVLDPYATVVGKSVSQ---KYLGSLSKSPSFDWGEDVSPNIPLEKLLVY 372
Query: 374 RLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFPSH 433
RLNVK FT +SS+L +++AGTFSG+ +K HLK LG NAVLLEPIF F E++GPYFP H
Sbjct: 373 RLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH 432
Query: 434 FFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTHTAEDGALKGIDDSSYYF 493
FFSP +I+GPS S SA+NS+K MVK+LH+ GIEV+LEVVFTHTA+ GAL+GIDDSSYY+
Sbjct: 433 FFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYY 492
Query: 494 ANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRP 553
R +++ KS L+CNYP+VQQL+L+SLRYWV EFH+DGFCFINASSLLRG HGE LSRP
Sbjct: 493 KGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 552
Query: 554 PLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGEGL 613
PLVEAIAFDPLL++TK+IAD WDP E + E R PHWKRWAE+N+++C +VR+F +G G+
Sbjct: 553 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGV 612
Query: 614 VSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELSWNCGEEGP 673
+S LATR+CGSGD+F+DGRGPAFSFN+I+RN+GL LVD+VSFS +LASELSWNCGEEG
Sbjct: 613 LSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGA 672
Query: 674 TNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRKAFDWNVLKTG 733
TN S VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS RK FDWN+L +
Sbjct: 673 TNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASA 732
Query: 734 FGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLR- 793
FGTQ TQFI+F++S R +R D+FQ R+FLK ENI WY +Q+ P+WEDP+ KFLA+ ++
Sbjct: 733 FGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKS 792
Query: 794 -ADKEENGSIIENPEPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGFFS 853
+++EE S+ E EP+S D+F+ FNASD ESV LP +G+ W RL+DTAL FPGFFS
Sbjct: 793 ESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFS 852
Query: 854 SDGEPV----PKTGSVTYEMQAHSCALFE 872
+GE V P V YEM+ +SC LFE
Sbjct: 853 VEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Lag0002435 vs. TAIR 10
Match:
AT1G03310.1 (debranching enzyme 1 )
HSP 1 Score: 955.7 bits (2469), Expect = 2.6e-278
Identity = 480/869 (55.24%), Postives = 625/869 (71.92%), Query Frame = 0
Query: 14 CVYNCGVAESFKLAVSDHHT-HGHKIKYQSGKMDEARMLALGSKVGFIKSSHRNLSKANA 73
C N G+ ++K + T +KIK S + R +G V S++ SK A
Sbjct: 13 CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQSYQ-FSKICA 72
Query: 74 TSGISVEKSGQRFGTSGKSKEHTKVATYLFRTEFGDLVNVYVGKTGSGFAVYIEMSSLQL 133
S S+E ++ + KV +Y FRT+ G LV V V K +++ + +SSL+L
Sbjct: 73 -SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLEL 132
Query: 134 ASIDGIPM-LSWGIYRSDSAIITP-NFQSSLPNETVGTTETPFVNASEGKFVVELEFDAK 193
+ D + + WG+YRSDS+ P +F++S + TTET FV +S + ++ LEFD K
Sbjct: 133 SGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGK 192
Query: 194 HVPFYLSFLLKYPLGVDPGSSEIRSHRKTNFCIPVGFGRGYPSPLGLSIS-GDGSINFSI 253
PFYLSF LK G DP E+ +HR T+FCIPVGF G+P PLGLS D S NFS
Sbjct: 193 ESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSF 252
Query: 254 FSSTAESVVLCLYDDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSYGYRCKGSKF 313
FS ++ +VVLCLYDDST++KP LELDLDPY+NR+G++WHAS + +FV YGYRCK +
Sbjct: 253 FSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAH 312
Query: 314 HENKDKVEVSRVLLDPYAKILTVSTPKTCGQGLGQICKVPAFDWDGEVCPNLPMEKLFVY 373
+ VE ++LDPYA ++ S + + LG + K P+FDW +V PN+P+EKL VY
Sbjct: 313 SKEDVDVEGEPIVLDPYATVVGKSVSQ---KYLGSLSKSPSFDWGEDVSPNIPLEKLLVY 372
Query: 374 RLNVKRFTVDKSSQLSTDIAGTFSGLTKKFLHLKNLGVNAVLLEPIFPFDEKEGPYFPSH 433
RLNVK FT +SS+L +++AGTFSG+ +K HLK LG NAVLLEPIF F E++GPYFP H
Sbjct: 373 RLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFH 432
Query: 434 FFSPTNIFGPSGSSISAINSLKEMVKELHANGIEVILEVVFTHTAEDGALKGIDDSSYYF 493
FFSP +I+GPS S SA+NS+K MVK+LH+ GIEV+LEVVFTHTA+ GAL+GIDDSSYY+
Sbjct: 433 FFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYY 492
Query: 494 ANRVANVEGKSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRP 553
R +++ KS L+CNYP+VQQL+L+SLRYWV EFH+DGFCFINASSLLRG HGE LSRP
Sbjct: 493 KGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 552
Query: 554 PLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGEGL 613
PLVEAIAFDPLL++TK+IAD WDP E + E R PHWKRWAE+N+++C +VR+F +G G+
Sbjct: 553 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGV 612
Query: 614 VSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELSWNCGEEGP 673
+S LATR+CGSGD+F+DGRGPAFSFN+I+RN+GL LVD+VSFS +LASELSWNCGEEG
Sbjct: 613 LSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGA 672
Query: 674 TNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRKAFDWNVLKTG 733
TN S VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS RK FDWN+L +
Sbjct: 673 TNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASA 732
Query: 734 FGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLR- 793
FGTQ TQFI+F++S R +R D+FQ R+FLK ENI WY +Q+ P+WEDP+ KFLA+ ++
Sbjct: 733 FGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKS 792
Query: 794 -ADKEENGSIIENPEPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGFFS 853
+++EE S+ E EP+S D+F+ FNASD ESV LP +G+ W RL+DTAL FPGFFS
Sbjct: 793 ESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFS 852
Query: 854 SDGEPV----PKTGSVTYEMQAHSCALFE 872
+GE V P V YEM+ +SC LFE
Sbjct: 853 VEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Lag0002435 vs. TAIR 10
Match:
AT4G09020.1 (isoamylase 3 )
HSP 1 Score: 373.2 bits (957), Expect = 5.6e-103
Identity = 248/679 (36.52%), Postives = 347/679 (51.10%), Query Frame = 0
Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----DDSTSEKPVLELDLDPYINRSG 289
G SPLG+S D INF++FS A SV LCL D T + ++EL LDP +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 290 NIWHASFEGAS-NFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKILT-VSTPKTCGQGL 349
+ WH E N V YGYR G + + + S +LLDPYAK++ S+ Q
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKF 207
Query: 350 GQI-----CKVPAFDW-DGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLT 409
Q + FDW D PN+P + L +Y +NV+ FT D+SS + I G++ G
Sbjct: 208 AQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFI 267
Query: 410 KKFLHLKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIFGP-SG 469
+K HL++LG+NAV L P+F FDE E Y +FF+P + + G
Sbjct: 268 EKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEG 327
Query: 470 SSISAINSLKEMVKELHANGIEVILEVVFTHTAE-------DGALKGIDDSSYYFA---N 529
I A KEMVK LH+ GIEVIL+VV+ HT E + +GID+ YY N
Sbjct: 328 DPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNN 387
Query: 530 RVANVEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGHHGELLSRPP 589
++ N G + L+CN+P+V +L+LDSLR+WV E+H+DGF F AS L R G LS PP
Sbjct: 388 QLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPP 447
Query: 590 LVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRWAEVNSKFCNDVRSFFKGE-GL 649
L+ AIA D +LS+ KIIA+ WD + + P+W RWAE N + +DVR F KG+ G+
Sbjct: 448 LIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGM 507
Query: 650 VSSLATRLCGSGDIFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSKLA 709
S ATR+ GS D++ + R P NF+ + G L DLVS++ +
Sbjct: 508 KGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCN 567
Query: 710 SELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-------QSSG 769
SWNCG EG T ++ + R +Q++NF L +S G P++ MGDE G S G
Sbjct: 568 DNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYG 627
Query: 770 GSVAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQSRNFLKGENIDWYGTNQ 829
+ N+ F W L +F + + FR + + NFL I W+ N
Sbjct: 628 HDTSLNN---FQWKELDAK-KQNHFRFFSEVIKFRHSH-HVLKHENFLTQGEITWHEDN- 687
Query: 830 SPPQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNASDQSESVALPEPSEGTS 850
W++ KFLA L + DI++AFNA D +P+P G
Sbjct: 688 ----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQ 743
BLAST of Lag0002435 vs. TAIR 10
Match:
AT2G39930.1 (isoamylase 1 )
HSP 1 Score: 335.5 bits (859), Expect = 1.3e-91
Identity = 229/689 (33.24%), Postives = 337/689 (48.91%), Query Frame = 0
Query: 230 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YDDSTSEKPVLELDLDPYINRSGNIW 289
G PSP G ++ DG +NFS++S+ + S +CL D K E+ LDP NR+G++W
Sbjct: 72 GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131
Query: 290 HASFEGASNFVSYGYRCKGSKFHENKDKVEVSRVLLDPYAKIL-------TVSTPKTCGQ 349
H G + YGYR G E + S +LLDPYAK + + C
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWP 191
Query: 350 GLGQICKVPA----FDWDGEVCPNLPMEKLFVYRLNVKRFTVDKSSQLSTDIAGTFSGLT 409
+ C VP FDW+G++ LP + L +Y ++V+ FT +SS++ + GT+ G+
Sbjct: 192 QMA--CMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGVA 251
Query: 410 KKFLHLKNLGVNAVLLEPIFPFDEKE---------------GPYFPSHFFSPTNIFGPSG 469
+K HLK LG+N + L P F+E E Y FFSP + +
Sbjct: 252 EKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASAS 311
Query: 470 SSI---SAINSLKEMVKELHANGIEVILEVVFTHTAEDG------ALKGIDDSSYYF--- 529
S+ AIN K +VKE H GIEVI++VV HTAE + +G+D+S YY
Sbjct: 312 SNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAP 371
Query: 530 ANRVANVEG-KSALSCNYPIVQQLLLDSLRYWVAEFHIDGFCFINASSLLRGH------- 589
N G + +CN+P+V+Q +LD LRYWV E H+DGF F S + R
Sbjct: 372 KGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAAN 431
Query: 590 ------HGELL------SRPPLVEAIAFDPLLSKTKIIADFWDPQESVTNETRLPHWKRW 649
G+LL S PP+++ I+ DP+L K+IA+ WD + PHW W
Sbjct: 432 VYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWD-AGGLYQVGMFPHWGIW 491
Query: 650 AEVNSKFCNDVRSFFKG-EGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNAGLPLVDL 709
+E N KF + VR F KG +G + A LCGS +++ GR P S NFI + G L DL
Sbjct: 492 SEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADL 551
Query: 710 VSFSS-SKLAS----------ELSWNCGEEGPTNNSEVLEKRLKQIRNFIFVLFVSLGVP 769
V++++ + LA+ SWNCGEEG + V R +Q+RNF L VS GVP
Sbjct: 552 VTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVP 611
Query: 770 VLNMGDECGQSSGGS----VAFNDRKAFDWNVLKTGFGTQTTQFITFLSSFRGKRFDLFQ 829
++ MGDE G + GG+ N F W+ K + +F L FR + +
Sbjct: 612 MIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDE-CESLG 671
Query: 830 SRNFLKGENIDWYGTNQSPPQWEDPSCKFLAVMLRADKEENGSIIENPEPRSDIFMAFNA 843
+F + + W+G P W + S +F+A S++++ + +I++AFN
Sbjct: 672 LNDFPTAKRLQWHGLAPEIPNWSETS-RFVAF----------SLVDS--VKKEIYVAFNT 731
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038898155.1 | 0.0e+00 | 85.62 | isoamylase 2, chloroplastic [Benincasa hispida] | [more] |
| XP_022150231.1 | 0.0e+00 | 83.79 | isoamylase 2, chloroplastic [Momordica charantia] | [more] |
| KAG7014422.1 | 0.0e+00 | 83.26 | Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| XP_008451396.1 | 0.0e+00 | 83.14 | PREDICTED: isoamylase 2, chloroplastic [Cucumis melo] | [more] |
| KAA0057598.1 | 0.0e+00 | 83.03 | isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo v... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q8L735 | 3.7e-277 | 55.24 | Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2 | [more] |
| Q6AU80 | 9.3e-220 | 52.22 | Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=... | [more] |
| B9G434 | 4.6e-102 | 36.10 | Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... | [more] |
| Q9M0S5 | 7.8e-102 | 36.52 | Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2 | [more] |
| D0TZF0 | 2.1e-94 | 33.91 | Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DAX4 | 0.0e+00 | 83.79 | isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 ... | [more] |
| A0A1S3BRF8 | 0.0e+00 | 83.14 | isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1 | [more] |
| A0A5A7UR47 | 0.0e+00 | 83.03 | Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 P... | [more] |
| A0A6J1GPU3 | 0.0e+00 | 83.26 | isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145... | [more] |
| A0A0A0K551 | 0.0e+00 | 81.90 | Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 S... | [more] |
