Lag0002370 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002370 vs. NCBI nr
Match: XP_022960107.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 690/733 (94.13%), Postives = 710/733 (96.86%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASI+FRRKL N+P LLRVRLFS SNSN EPAR IEKILIANRGEIACRI RTARRLGIQ
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNSNTEPARRIEKILIANRGEIACRIARTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAASI++AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAASIIEAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQ CED+GLTFIGPP SAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE
Sbjct: 121 SADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI QS
Sbjct: 301 YYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM
Sbjct: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKDCLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLFVDPSNLVMAK+AY+AAGLNARLAAACL+SLELSKLNEKS G GIHSIWYSPPPFRV
Sbjct: 481 DDLFVDPSNLVMAKQAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RTIE+EWEN YD SVSKPFALTITYQQDGG L+ETGENGSSAVEVKVTHLGKH F
Sbjct: 541 HHCARRTIEYEWENPYDCSVSKPFALTITYQQDGGLLIETGENGSSAVEVKVTHLGKHKF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDGIIMEVRLAIYSKDQTKHVHIWHGS HHHFKQ LGVNLSE+DESQHKPGFEAAS+
Sbjct: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAASH 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HPEGTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVA GQ
Sbjct: 661 HPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAGQ 720

Query: 721 QVSDGSILFCVKE 734
           QVSDGSILF VK+
Sbjct: 721 QVSDGSILFSVKK 733

BLAST of Lag0002370 vs. NCBI nr
Match: XP_023513665.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1387.1 bits (3589), Expect = 0.0e+00
Identity = 689/733 (94.00%), Postives = 709/733 (96.73%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASI+FRRKL N+P LLRVRLFS SNSN EPAR IEKILIANRGEIACRI RTARRLGIQ
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNSNTEPARRIEKILIANRGEIACRIARTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNA SI++AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAVSIIEAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQ CED+GLTFIGPP SAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE
Sbjct: 121 SADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI QS
Sbjct: 301 YYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM
Sbjct: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKDCLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLFVDPSNLVMAKEAY+AAGLNARLAAACL+SLELSKLNEKS G GIHSIWYSPPPFRV
Sbjct: 481 DDLFVDPSNLVMAKEAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RTIE+EWEN YDSSVSKPFALTITYQ DGG L+ETGENGSSAVEVKVTHLGKH F
Sbjct: 541 HHCARRTIEYEWENPYDSSVSKPFALTITYQLDGGLLIETGENGSSAVEVKVTHLGKHKF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDGIIMEVRLAIYSKD+TKHVHIWHGS HHHFKQ LGVNLSE+DESQHKPGFEAAS+
Sbjct: 601 RVEVDGIIMEVRLAIYSKDRTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAASH 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HPEGTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVA GQ
Sbjct: 661 HPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAGQ 720

Query: 721 QVSDGSILFCVKE 734
           QVSDGSILF VK+
Sbjct: 721 QVSDGSILFSVKK 733

BLAST of Lag0002370 vs. NCBI nr
Match: KAG7025511.1 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 688/732 (93.99%), Postives = 706/732 (96.45%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASI+FRRKL N+P LLRVRLFS SNSN EPAR IEKILIANRGEIACRI RTARRLGIQ
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNSNTEPARRIEKILIANRGEIACRIARTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAASI++AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAASIIEAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQ CED+GLTFIGPP SAIRHMGDKSASKRIMGAAGVPLVPGYHGT QDIDTMKLE
Sbjct: 121 SADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTEQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI QS
Sbjct: 301 YYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGD VSVHYDPM
Sbjct: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDGVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKDCLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLFVDPSNLVMAKEAY+AAGLNARLAAACL+SLELSKLNEKS G GIHSIWYSPPPFRV
Sbjct: 481 DDLFVDPSNLVMAKEAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RTIE+EWEN YDSSVSKP ALTITYQQDGG L+ETGENGSSA EVKVTHLGKH F
Sbjct: 541 HHCARRTIEYEWENPYDSSVSKPCALTITYQQDGGLLIETGENGSSAAEVKVTHLGKHKF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDGIIMEVRLAIYSKDQTKHVHIWHGS HHHFKQ LGVNLSE+DESQHKPGFEAAS+
Sbjct: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAASH 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HPEGTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVA GQ
Sbjct: 661 HPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAGQ 720

Query: 721 QVSDGSILFCVK 733
           QVSDGSILF VK
Sbjct: 721 QVSDGSILFSVK 732

BLAST of Lag0002370 vs. NCBI nr
Match: XP_023004327.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 686/733 (93.59%), Postives = 709/733 (96.73%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASI+FRRKLPN+P LLRVRLFS SNS  EPAR IEKILIANRGEIACRI RTARRLGIQ
Sbjct: 1   MASIVFRRKLPNKPFLLRVRLFSQSNSYTEPARRIEKILIANRGEIACRIARTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAATIIEAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQ CED+GLTFIGPP SAIRHMGDKSASKRIMGAAGVPLVPGYHGT QDIDTMKLE
Sbjct: 121 SADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTEQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI QS
Sbjct: 301 YYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPL GHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM
Sbjct: 361 QVPLFGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKDCLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLFVDPSNLVMAKEAY+AA LNARLAAACL+SLELSKLNEKS G GIHSIWYSPPPFRV
Sbjct: 481 DDLFVDPSNLVMAKEAYDAAWLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RTIE+EWEN YDSSVSKPFALTITYQQDGG L+ETGE+GSSAVEVKVTHLGK+ F
Sbjct: 541 HHCARRTIEYEWENPYDSSVSKPFALTITYQQDGGLLIETGEHGSSAVEVKVTHLGKYKF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDGIIMEVRLAIYSKDQTKH+HIWHGSHHHHFKQ LGVNLSE+DESQHKPGFEAAS+
Sbjct: 601 RVEVDGIIMEVRLAIYSKDQTKHIHIWHGSHHHHFKQKLGVNLSEEDESQHKPGFEAASH 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HPEGTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVA GQ
Sbjct: 661 HPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAGQ 720

Query: 721 QVSDGSILFCVKE 734
           QVSDGSILF VK+
Sbjct: 721 QVSDGSILFSVKK 733

BLAST of Lag0002370 vs. NCBI nr
Match: XP_022150199.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 1374.4 bits (3556), Expect = 0.0e+00
Identity = 680/734 (92.64%), Postives = 706/734 (96.19%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNS-NIEPARPIEKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKLPN+P LLRVRLFS SNS   EP R I+KILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLC+DE LTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQ 360
           GYY+AGTVEFIVDTI+G+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI+Q
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQY 480
           MIAKLVVWGENR AALVKLKDCL KFEVAGVPTNINFLLKL+NHYAFE GDVETHFIE+Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIERY 480

Query: 481 KDDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAKEAYE A LNARLAAAC++ LE SKLN+KS G GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540

Query: 541 VHHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHN 600
           VHHSA+RT+EFEWEN YDSSVSKPFAL ITYQQDGGFL+ETGENGSSAV VKV  LGKHN
Sbjct: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKHN 600

Query: 601 FRVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKDQTKHVHIWHGSHHHHFKQ LG++LS++DESQHKPGFEAAS
Sbjct: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660

Query: 661 NHPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVG 720
           NHP+GTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVA G
Sbjct: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFCVKE 734
           QQVSDGSILF VK+
Sbjct: 721 QQVSDGSILFSVKK 734

BLAST of Lag0002370 vs. ExPASy Swiss-Prot
Match: Q42777 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Glycine max OX=3847 GN=MCCA PE=1 SV=2)

HSP 1 Score: 1067.0 bits (2758), Expect = 9.7e-311
Identity = 537/734 (73.16%), Postives = 613/734 (83.51%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MAS+   R+       +R R FS   S+      IEKIL+ANRGEIACRI RTARRLGIQ
Sbjct: 1   MASLALLRRTTLSHSHVRARAFSEGKSS--NRHRIEKILVANRGEIACRITRTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAV+SDADRDSLHV +ADEA+ IGP PARLSYLN ASIV AA R+GAQAIHPGYGFLSE
Sbjct: 61  TVAVYSDADRDSLHVATADEAIRIGPPPARLSYLNGASIVDAAIRSGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFA+LCE+ GLTFIGPP SAIR MGDKSASKRIMGAAGVPLVPGYHG  QDI+ MKLE
Sbjct: 121 SADFAKLCEESGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGYDQDIEKMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           AD+IGYP+LIKPTHGGGGKGMRIVH+P+EFV++FL AQREAAASFG++TILLEKYIT+PR
Sbjct: 181 ADRIGYPVLIKPTHGGGGKGMRIVHTPDEFVESFLAAQREAAASFGVNTILLEKYITRPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGD HGN+LHLYERDCSVQRRHQKIIEEAPAPN+  DFR+ LG AAVSAAKAV 
Sbjct: 241 HIEVQIFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISADFRAQLGVAAVSAAKAVN 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDT++ +FYFMEMNTRLQVEHPVTEMIVGQDLVEWQI VANGE+LP++QS
Sbjct: 301 YYNAGTVEFIVDTVSDEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQILVANGEALPLSQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPL GHAFEARIYAENV KGFLPATG LHHY  VPVS ++RVETGV++GD VS+HYDPM
Sbjct: 361 QVPLSGHAFEARIYAENVQKGFLPATGVLHHY-HVPVSSAVRVETGVKEGDKVSMHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKD L+KF+VAG+PTN+NFL KLANH AF  G+VETHFI+ YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDSLSKFQVAGLPTNVNFLQKLANHRAFAIGNVETHFIDNYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKL-NEKSYGTGIHSIWYSPPPFR 540
           +DLFVD +N V  KEAYEAA LNA L AACLI  E   L      G+ +  IWYS PPFR
Sbjct: 481 EDLFVDANNSVSVKEAYEAARLNASLVAACLIEKEHFILARNPPGGSSLLPIWYSSPPFR 540

Query: 541 VHHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHN 600
           +HH AKR +E EW+N Y S  SK   LTITYQ DG +L+ET +NGS  +EVK T++  + 
Sbjct: 541 IHHQAKRRMELEWDNEYGSGSSKIMKLTITYQPDGRYLIETEQNGSPVLEVKSTYVKDNY 600

Query: 601 FRVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAAS 660
           FRVE  G+I +V +A+YSKDQ +H+HIW GS HH+F++ LG+ LSED+ESQHKP  E ++
Sbjct: 601 FRVEAAGVINDVNVAVYSKDQIRHIHIWQGSCHHYFREKLGLELSEDEESQHKPKVETSA 660

Query: 661 NHPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVG 720
           N P+GTVVAPMAGLVVKVLV+N   V EGQ VLVLEAMKMEHVVKAP +G +HGL + VG
Sbjct: 661 N-PQGTVVAPMAGLVVKVLVENKTRVEEGQPVLVLEAMKMEHVVKAPSSGYVHGLQLMVG 720

Query: 721 QQVSDGSILFCVKE 734
           +QVSDGS+LF VK+
Sbjct: 721 EQVSDGSVLFSVKD 730

BLAST of Lag0002370 vs. ExPASy Swiss-Prot
Match: Q42523 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MCCA PE=1 SV=2)

HSP 1 Score: 1023.8 bits (2646), Expect = 9.4e-298
Identity = 519/727 (71.39%), Postives = 594/727 (81.71%), Query Frame = 0

Query: 7   RRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQTVAVFS 66
           RR +  +   + VR  S S S     + IEKIL+ANRGEIACRI+RTA+RLGIQTVAV+S
Sbjct: 10  RRNVRRKNHSMLVRYISGSASMKPKEQCIEKILVANRGEIACRIMRTAKRLGIQTVAVYS 69

Query: 67  DADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSESADFAQ 126
           DADRDSLHVKSADEAV IGP  ARLSYL+  +I++AA+RTGAQAIHPGYGFLSES+DFAQ
Sbjct: 70  DADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFAQ 129

Query: 127 LCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLEADKIGY 186
           LCED GLTFIGPP SAIR MGDKSASKRIMGAAGVPLVPGYHG  QDID MK EA+KIGY
Sbjct: 130 LCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIGY 189

Query: 187 PILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPRHIEVQI 246
           PI+IKPTHGGGGKGMRIV S  +F D+FLGAQREAAASFG++TILLEKYIT+PRHIEVQI
Sbjct: 190 PIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQI 249

Query: 247 FGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYNAGT 306
           FGD HGN+LHLYERDCSVQRRHQKIIEEAPAPN+ + FR++LGQAAVSAA+AVGYYNAGT
Sbjct: 250 FGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAGT 309

Query: 307 VEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQSQVPLLG 366
           VEFIVDT + QFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGE LP++QS+VP+ G
Sbjct: 310 VEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSG 369

Query: 367 HAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPMIAKLVV 426
           HAFEARIYAENVPKGFLPATG L+HY PV VS S+RVETGVEQGD VS+HYDPMIAKLVV
Sbjct: 370 HAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVV 429

Query: 427 WGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYKDDLFVD 486
           WG NR  ALVKLKDCL+ F+VAGVPTNINFL KLA+H  F  G+VETHFIE +K DLF D
Sbjct: 430 WGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFAD 489

Query: 487 PSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRVHHSAKR 546
            SN    + AY+A   +A L AAC+ ++E S  NE ++G  + SIWYS PPFRVHH AK+
Sbjct: 490 ESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGK-VPSIWYSNPPFRVHHEAKQ 549

Query: 547 TIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNFRVEVDG 606
           TIE EW N  + + S   +L + YQ DG +L+E G N S ++E++VT  GK +FRVE  G
Sbjct: 550 TIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEG-NDSPSLELRVTRAGKCDFRVEAAG 609

Query: 607 IIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDES-QHKPGFEAASNHPEGT 666
           + M V LA Y KD  KH+HIWHGS HH FKQ +G+  SED+E  QH+   E  S+HP GT
Sbjct: 610 LSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSE-TSSHPPGT 669

Query: 667 VVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQQVSDG 726
           +VAPMAGLVVKVLV+N  +V +GQ +LVLEAMKMEHVVKAP +G I  L V  GQQVSDG
Sbjct: 670 IVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDG 729

Query: 727 SILFCVK 733
           S LF +K
Sbjct: 730 SALFRIK 733

BLAST of Lag0002370 vs. ExPASy Swiss-Prot
Match: Q2QMG2 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=MCCA PE=2 SV=2)

HSP 1 Score: 981.9 bits (2537), Expect = 4.1e-285
Identity = 492/732 (67.21%), Postives = 585/732 (79.92%), Query Frame = 0

Query: 7   RRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQTVAVFS 66
           RR       LL  RL S S+S       +EK+L+ANRGEIACR++RTARRLGI TVAV+S
Sbjct: 11  RRSRHGGASLLLARLLSSSSSEAGGGGAVEKVLVANRGEIACRVMRTARRLGIPTVAVYS 70

Query: 67  DADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSESADFAQ 126
           DADR +LHV++ADEAV +GP PAR SYLNA++IV AA RTGA+AIHPGYGFLSESADFAQ
Sbjct: 71  DADRGALHVRAADEAVRLGPPPARESYLNASAIVDAALRTGAKAIHPGYGFLSESADFAQ 130

Query: 127 LCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLEADKIGY 186
           LC+ EGLTFIGPP SAIR MGDKSASKRIMGAAGVPLVPGYHG  QDI+ +KLEA+KIGY
Sbjct: 131 LCKAEGLTFIGPPPSAIRDMGDKSASKRIMGAAGVPLVPGYHGAEQDIELLKLEANKIGY 190

Query: 187 PILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPRHIEVQI 246
           P+LIKPTHGGGGKGMRIV  P +FVD+ L AQREAAASFGI+T+L+EKYITQPRHIEVQI
Sbjct: 191 PVLIKPTHGGGGKGMRIVQRPEDFVDSVLSAQREAAASFGINTLLVEKYITQPRHIEVQI 250

Query: 247 FGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYNAGT 306
           FGD HGN++HLYERDCS+QRRHQKIIEEAPAPNV   FRSH+G+AAVSAAKAVGYY+AGT
Sbjct: 251 FGDQHGNVIHLYERDCSLQRRHQKIIEEAPAPNVTAQFRSHIGEAAVSAAKAVGYYSAGT 310

Query: 307 VEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQSQVPLLG 366
           VEFIVDT++G+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIR+ANGE LP++Q QVPL G
Sbjct: 311 VEFIVDTLSGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRIANGECLPLSQEQVPLNG 370

Query: 367 HAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPMIAKLVV 426
           HAFEARIYAENVP+GFLPATG+LHHY PVP + ++RVETGVE+GD VS+HYDPMIAKLVV
Sbjct: 371 HAFEARIYAENVPRGFLPATGTLHHYRPVPSTATVRVETGVEEGDTVSMHYDPMIAKLVV 430

Query: 427 WGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYKDDLFVD 486
           WGE+R AALVKLK+ L+ F++AG+PTN+ FL +LA H AFE+G V+THFIE+Y++DL   
Sbjct: 431 WGESRNAALVKLKNSLSNFQIAGLPTNVGFLQELAGHSAFEKGLVDTHFIERYQNDLLST 490

Query: 487 PSNLVMAKEAYEAAGLNARLAAACL-----ISLELSKLNEKSYGTGIHSIWYSPPPFRVH 546
            +  +    ++EA  L A LAAAC+     +S E+S L++K       S+WY+ PPFR+H
Sbjct: 491 STQALSG--SHEAEELGAILAAACICKKDHVSSEVS-LHDKKL-----SMWYAHPPFRMH 550

Query: 547 HSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNFR 606
           H AKR +EFE +     S      L++TY+ DG + VET +  S  ++VKV   G H+FR
Sbjct: 551 HFAKRLMEFELDRELGGSSDDLLKLSVTYRSDGTYFVETEDGSSPGLDVKVDSRGDHDFR 610

Query: 607 VEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASNH 666
           V+V G+  +V LA YSKD   H+HIWHG HHHH++Q L    S DD SQ     EA S H
Sbjct: 611 VDVGGLQTDVTLAFYSKDNCNHIHIWHGKHHHHYRQTLRAEQSPDDSSQPSASSEARS-H 670

Query: 667 PEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQQ 726
           P+G+V+APMAGLVVKVL+K+G  V EGQ V+V+EAMKMEHVVKAP AG + GL    GQQ
Sbjct: 671 PKGSVLAPMAGLVVKVLLKDGARVEEGQPVMVMEAMKMEHVVKAPCAGYVEGLKATAGQQ 730

Query: 727 VSDGSILFCVKE 734
           V D S+LF VKE
Sbjct: 731 VFDSSVLFTVKE 733

BLAST of Lag0002370 vs. ExPASy Swiss-Prot
Match: Q54KE6 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mccA PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 4.5e-175
Identity = 344/721 (47.71%), Postives = 468/721 (64.91%), Query Frame = 0

Query: 20  RLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSAD 79
           R  ++ N +++  +PI KILIANRGEIACR++RTA+  G++TVAV+S+AD++SLHV  AD
Sbjct: 16  RYITNVNKDLK-IKPITKILIANRGEIACRVMRTAKSKGVKTVAVYSEADKNSLHVSMAD 75

Query: 80  EAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSESADFAQLCEDEGLTFIGPP 139
           E+  IGP+ A+ SYL    I+  A R+GAQAIHPGYGFLSE++DFA LCE EG+ FIGPP
Sbjct: 76  ESYLIGPAAAKESYLCGNKIIDVAKRSGAQAIHPGYGFLSENSDFADLCEREGIIFIGPP 135

Query: 140 ISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGK 199
             AI+ MG KSASK IM  AGVP +PGYHG  Q +  +K EA KIGYP+LIK   GGGGK
Sbjct: 136 SDAIKAMGSKSASKDIMIKAGVPTIPGYHGEDQSMSVLKSEAAKIGYPVLIKAVMGGGGK 195

Query: 200 GMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYE 259
           GMRIV    +  D    ++REA ASFG S +L+EKY+  PRH+E+Q+F D HGN +HL+E
Sbjct: 196 GMRIVEREEDLEDGVESSKREATASFGDSRVLVEKYLVHPRHVEIQVFADRHGNCVHLFE 255

Query: 260 RDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYNAGTVEFIVDTITGQFY 319
           RDCSVQRRHQKIIEEAPAP++ ++ R  +G AAV+AAKAVGY  AGTVEFI+ +    F+
Sbjct: 256 RDCSVQRRHQKIIEEAPAPHLSEELRKKMGDAAVAAAKAVGYVGAGTVEFIL-SADNSFF 315

Query: 320 FMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQSQVPLLGHAFEARIYAENVP 379
           FMEMNTRLQVEHP+TEMI  QDLVEWQ++VA  ++LP+ Q Q+ + GH+FEARIYAEN  
Sbjct: 316 FMEMNTRLQVEHPITEMITKQDLVEWQLKVAESQTLPMEQEQLKIHGHSFEARIYAENPD 375

Query: 380 KGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRTAALVKLK 439
             FLP TG L H      S ++RVETGV QGD VSV+YDPMIAKLVVW ++R  AL  L+
Sbjct: 376 SDFLPGTGKLAHLSTPTPSDTLRVETGVRQGDEVSVYYDPMIAKLVVWDQDREKALRYLR 435

Query: 440 DCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYKDDLFVDPSNLVMAKEAYEA 499
           + L ++ + G+ TNI+FL +L+ H +F  G+VET FI  +++ L        MA +A   
Sbjct: 436 NALDEYHIIGLNTNISFLKRLSTHPSFMAGEVETGFIPIHRESL--------MAPQA-PM 495

Query: 500 AGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRVHHSAKRTIEFEWENHYDSS 559
           +  +  LAA  L+  E+++   K      +S W S   FR++H+ K+ ++F   N  D+ 
Sbjct: 496 SDDSLALAATSLLLKEITQQKSKE---DPNSPWSSLGGFRINHNLKKQVKF---NQKDNK 555

Query: 560 VSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNFRVEVD-GIIMEVRLA-IYS 619
           V       +  +  GG        G +A        GKHNF+V +D G ++EV  A +  
Sbjct: 556 V------VVNVEFIGG--------GGAAAN------GKHNFKVTLDNGNVVEVLDAKLNQ 615

Query: 620 KDQTKHVHIWHGSHHHHFKQ----------NLGVNLSEDDESQHKPGFEAASNHPEGTVV 679
            ++T   H+ +G  +++ K           N G     D     KP     ++   G++V
Sbjct: 616 NNETISAHV-NGRFYNNIKSVIVKDTLTIFNEGQQYQLDIPQDVKP---KGADGVLGSLV 675

Query: 680 APMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQQVSDGSI 729
           +PM G + KV+V  GD V +GQ +L++EAMKMEH +++P+ G++  L   V + V D   
Sbjct: 676 SPMPGKITKVMVNVGDMVKKGQPILLMEAMKMEHTIRSPIDGKVESLPYNVNEIVEDKKT 695

BLAST of Lag0002370 vs. ExPASy Swiss-Prot
Match: Q99MR8 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Mccc1 PE=1 SV=2)

HSP 1 Score: 612.1 bits (1577), Expect = 8.5e-174
Identity = 331/695 (47.63%), Postives = 440/695 (63.31%), Query Frame = 0

Query: 31  PARPIEKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPAR 90
           P   I K+LIANRGEIACR+IRTA+++G+Q+VAV+S+ADR+S+HV  ADEA  IGP+P++
Sbjct: 41  PGGSITKVLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQ 100

Query: 91  LSYLNAASIVKAASRTGAQAIHPGYGFLSESADFAQLCEDEGLTFIGPPISAIRHMGDKS 150
            SYL    I++ A  + AQAIHPGYGFLSE+ +FA+LC+ EG+ FIGPP SAIR MG KS
Sbjct: 101 QSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSSAIRDMGIKS 160

Query: 151 ASKRIMGAAGVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEF 210
            SK IM AAGVP+V GYHG  Q    ++  A KIGYP++IK   GGGGKGMRIV S  EF
Sbjct: 161 TSKSIMAAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKGMRIVRSEREF 220

Query: 211 VDAFLGAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQK 270
            +    A+REA  SF    +L+EK++  PRH+EVQ+FGD HGN ++L+ERDCSVQRRHQK
Sbjct: 221 QEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQK 280

Query: 271 IIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYNAGTVEFIVDTITGQFYFMEMNTRLQVE 330
           IIEEAPAP +  + R  LG+AAV AAKAV Y  AGTVEFI+D+    FYFMEMNTRLQVE
Sbjct: 281 IIEEAPAPGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDS-RHNFYFMEMNTRLQVE 340

Query: 331 HPVTEMIVGQDLVEWQIRVANGESLPINQSQVPLLGHAFEARIYAENVPKGFLPATGSLH 390
           HPVTEMI G DLVEWQ+R+A GE +P++Q ++PL GHAFEARIYAE+    F+P  G L 
Sbjct: 341 HPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLV 400

Query: 391 HYCPVPVSQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRTAALVKLKDCLTKFEVAGV 450
           H        S R+ETGV QGD VSVHYDPMIAKLVVW  +R +AL KL+ CL ++ + G+
Sbjct: 401 HLSTPSADMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYCLHQYNIVGL 460

Query: 451 PTNINFLLKLANHYAFERGDVETHFIEQYKDDLFVDPSNLVMAKEAYEAAGLNARLAAAC 510
            +N++FLL+L+ H  FE G+V T FI Q+  DL   PS+  +AKE+   A L   L    
Sbjct: 461 RSNVDFLLRLSGHPEFEAGNVHTDFIPQHHKDLL--PSHSTIAKESVCQAALGLIL---- 520

Query: 511 LISLELSKLNEKSYGTGIHS-IWYSPPPFRVHHSAKRTIEFEW-ENHYDSSVSKPFALTI 570
                  K  E +    +H+   +SP  F    S+ R +   +  N    S      + +
Sbjct: 521 -------KEKEMTSAFKLHTQDQFSPFSF----SSGRRLNISYTRNMTLRSGKSDIVIAV 580

Query: 571 TYQQDGGFLVETGENGSSAVEVKVTHLGKHNFRVEVDGIIMEVRLAIYSKDQTKHVHIWH 630
           TY +DG + ++        +    +  G    +  ++G+  + +  +   D T H+    
Sbjct: 581 TYNRDGSYDMQIDNKSFRVLGDLSSEDGCTYLKSSINGVARKSKFILL--DNTVHLFSME 640

Query: 631 GSHHHHFKQNLGVNLSEDDESQHKPGFEAASNHPEGTVVAPMAGLVVKVLVKNGDEVGEG 690
           GS        +G+ +        K     ++   +G  +APM G + KV VK GD V  G
Sbjct: 641 GS------IEVGIPVP-------KYLSPVSAEGAQGGTIAPMTGTIEKVFVKAGDRVKAG 700

Query: 691 QSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQQVS 724
            S++V+ AMKMEH +KAP  G+I  +  + G Q +
Sbjct: 701 DSLMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQAN 702

BLAST of Lag0002370 vs. ExPASy TrEMBL
Match: A0A6J1H6G1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460955 PE=4 SV=1)

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 690/733 (94.13%), Postives = 710/733 (96.86%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASI+FRRKL N+P LLRVRLFS SNSN EPAR IEKILIANRGEIACRI RTARRLGIQ
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNSNTEPARRIEKILIANRGEIACRIARTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAASI++AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAASIIEAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQ CED+GLTFIGPP SAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE
Sbjct: 121 SADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI QS
Sbjct: 301 YYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM
Sbjct: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKDCLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLFVDPSNLVMAK+AY+AAGLNARLAAACL+SLELSKLNEKS G GIHSIWYSPPPFRV
Sbjct: 481 DDLFVDPSNLVMAKQAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RTIE+EWEN YD SVSKPFALTITYQQDGG L+ETGENGSSAVEVKVTHLGKH F
Sbjct: 541 HHCARRTIEYEWENPYDCSVSKPFALTITYQQDGGLLIETGENGSSAVEVKVTHLGKHKF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDGIIMEVRLAIYSKDQTKHVHIWHGS HHHFKQ LGVNLSE+DESQHKPGFEAAS+
Sbjct: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAASH 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HPEGTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVA GQ
Sbjct: 661 HPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAGQ 720

Query: 721 QVSDGSILFCVKE 734
           QVSDGSILF VK+
Sbjct: 721 QVSDGSILFSVKK 733

BLAST of Lag0002370 vs. ExPASy TrEMBL
Match: A0A6J1KQ45 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111497679 PE=4 SV=1)

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 686/733 (93.59%), Postives = 709/733 (96.73%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASI+FRRKLPN+P LLRVRLFS SNS  EPAR IEKILIANRGEIACRI RTARRLGIQ
Sbjct: 1   MASIVFRRKLPNKPFLLRVRLFSQSNSYTEPARRIEKILIANRGEIACRIARTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAATIIEAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQ CED+GLTFIGPP SAIRHMGDKSASKRIMGAAGVPLVPGYHGT QDIDTMKLE
Sbjct: 121 SADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTEQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YYNAGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI QS
Sbjct: 301 YYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQS 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPL GHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM
Sbjct: 361 QVPLFGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR AALVKLKDCLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLFVDPSNLVMAKEAY+AA LNARLAAACL+SLELSKLNEKS G GIHSIWYSPPPFRV
Sbjct: 481 DDLFVDPSNLVMAKEAYDAAWLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RTIE+EWEN YDSSVSKPFALTITYQQDGG L+ETGE+GSSAVEVKVTHLGK+ F
Sbjct: 541 HHCARRTIEYEWENPYDSSVSKPFALTITYQQDGGLLIETGEHGSSAVEVKVTHLGKYKF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDGIIMEVRLAIYSKDQTKH+HIWHGSHHHHFKQ LGVNLSE+DESQHKPGFEAAS+
Sbjct: 601 RVEVDGIIMEVRLAIYSKDQTKHIHIWHGSHHHHFKQKLGVNLSEEDESQHKPGFEAASH 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HPEGTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVA GQ
Sbjct: 661 HPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAGQ 720

Query: 721 QVSDGSILFCVKE 734
           QVSDGSILF VK+
Sbjct: 721 QVSDGSILFSVKK 733

BLAST of Lag0002370 vs. ExPASy TrEMBL
Match: A0A6J1D9A1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018426 PE=4 SV=1)

HSP 1 Score: 1374.4 bits (3556), Expect = 0.0e+00
Identity = 680/734 (92.64%), Postives = 706/734 (96.19%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNS-NIEPARPIEKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKLPN+P LLRVRLFS SNS   EP R I+KILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLC+DE LTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQ 360
           GYY+AGTVEFIVDTI+G+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI+Q
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQY 480
           MIAKLVVWGENR AALVKLKDCL KFEVAGVPTNINFLLKL+NHYAFE GDVETHFIE+Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIERY 480

Query: 481 KDDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAKEAYE A LNARLAAAC++ LE SKLN+KS G GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540

Query: 541 VHHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHN 600
           VHHSA+RT+EFEWEN YDSSVSKPFAL ITYQQDGGFL+ETGENGSSAV VKV  LGKHN
Sbjct: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKHN 600

Query: 601 FRVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKDQTKHVHIWHGSHHHHFKQ LG++LS++DESQHKPGFEAAS
Sbjct: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660

Query: 661 NHPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVG 720
           NHP+GTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVA G
Sbjct: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFCVKE 734
           QQVSDGSILF VK+
Sbjct: 721 QQVSDGSILFSVKK 734

BLAST of Lag0002370 vs. ExPASy TrEMBL
Match: A0A6J1DA31 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018426 PE=4 SV=1)

HSP 1 Score: 1369.4 bits (3543), Expect = 0.0e+00
Identity = 679/735 (92.38%), Postives = 706/735 (96.05%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNS-NIEPARPIEKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKLPN+P LLRVRLFS SNS   EP R I+KILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLC+DE LTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQ 360
           GYY+AGTVEFIVDTI+G+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI+Q
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQS-IRVETGVEQGDAVSVHYD 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQS +RVETGVEQGDAVSVHYD
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSTVRVETGVEQGDAVSVHYD 420

Query: 421 PMIAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQ 480
           PMIAKLVVWGENR AALVKLKDCL KFEVAGVPTNINFLLKL+NHYAFE GDVETHFIE+
Sbjct: 421 PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIER 480

Query: 481 YKDDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPF 540
           YKDDLFVDPSN VMAKEAYE A LNARLAAAC++ LE SKLN+KS G GIHSIWYSPPPF
Sbjct: 481 YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF 540

Query: 541 RVHHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKH 600
           RVHHSA+RT+EFEWEN YDSSVSKPFAL ITYQQDGGFL+ETGENGSSAV VKV  LGKH
Sbjct: 541 RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKH 600

Query: 601 NFRVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAA 660
           NFRVEVDG+IMEVRLAIYSKDQTKHVHIWHGSHHHHFKQ LG++LS++DESQHKPGFEAA
Sbjct: 601 NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA 660

Query: 661 SNHPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAV 720
           SNHP+GTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVA 
Sbjct: 661 SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA 720

Query: 721 GQQVSDGSILFCVKE 734
           GQQVSDGSILF VK+
Sbjct: 721 GQQVSDGSILFSVKK 735

BLAST of Lag0002370 vs. ExPASy TrEMBL
Match: A0A5A7VDQ8 (Methylcrotonoyl-CoA carboxylase subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001030 PE=4 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 659/732 (90.03%), Postives = 691/732 (94.40%), Query Frame = 0

Query: 1   MASIIFRRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQ 60
           MASIIFRRKLP  P LLR RLFSHSNSN  P   I+KILIANRGEIACRIIRTARRLGIQ
Sbjct: 1   MASIIFRRKLPTTPSLLRFRLFSHSNSNAHPPPRIDKILIANRGEIACRIIRTARRLGIQ 60

Query: 61  TVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSE 120
           TVAVFSDADRDSLHVKSADEAVHIGP PARLSYLNA SIV AASRTGAQAIHPGYGFLSE
Sbjct: 61  TVAVFSDADRDSLHVKSADEAVHIGPPPARLSYLNAPSIVDAASRTGAQAIHPGYGFLSE 120

Query: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180
           SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE
Sbjct: 121 SADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLE 180

Query: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPR 240
           ADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQPR
Sbjct: 181 ADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQPR 240

Query: 241 HIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300
           HIEVQIFGDTHGNILHL ERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG
Sbjct: 241 HIEVQIFGDTHGNILHLNERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVG 300

Query: 301 YYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQS 360
           YY+AGTVEFIVDTI+GQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI Q+
Sbjct: 301 YYSAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPITQA 360

Query: 361 QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPM 420
           QVPLLGHAFEARIYAENV KGFLPATG+LHHYCPVPVSQS+RVETGVEQGDAVSVHYDPM
Sbjct: 361 QVPLLGHAFEARIYAENVSKGFLPATGTLHHYCPVPVSQSVRVETGVEQGDAVSVHYDPM 420

Query: 421 IAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYK 480
           IAKLVVWGENR+AALVKLK CLTKFEVAGVPTNINFLLKLANH+AFERGDVETHFIE YK
Sbjct: 421 IAKLVVWGENRSAALVKLKHCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHYK 480

Query: 481 DDLFVDPSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRV 540
           DDLF DPSNL+MAKEAYEA G NA LAAAC++SLE SKL E   G  +HSIWYSPPPFRV
Sbjct: 481 DDLFGDPSNLLMAKEAYEAGGFNACLAAACIVSLEHSKLAENFSGNDLHSIWYSPPPFRV 540

Query: 541 HHSAKRTIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNF 600
           HH A+RT+EF  EN YDSS SKPF LTITYQQDGGFL+E+GE+GSSAVEVKV+HLGKHNF
Sbjct: 541 HHCARRTVEFALENQYDSSASKPFPLTITYQQDGGFLIESGESGSSAVEVKVSHLGKHNF 600

Query: 601 RVEVDGIIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDESQHKPGFEAASN 660
           RVEVDG+IMEVRLAIYS+D+ KHVHIWHGSHHHHFKQ LG+++ ++DESQHKPGFEAASN
Sbjct: 601 RVEVDGVIMEVRLAIYSEDRIKHVHIWHGSHHHHFKQKLGIDVVDEDESQHKPGFEAASN 660

Query: 661 HPEGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQ 720
           HP+GTVVAPMAGLVVKVL+KNGDEVGEGQ VLVLEAMKMEHVVK P+AGQI+GLHVA GQ
Sbjct: 661 HPQGTVVAPMAGLVVKVLMKNGDEVGEGQPVLVLEAMKMEHVVKTPIAGQIYGLHVAPGQ 720

Query: 721 QVSDGSILFCVK 733
           QV+DGSILF VK
Sbjct: 721 QVTDGSILFSVK 732

BLAST of Lag0002370 vs. TAIR 10
Match: AT1G03090.2 (methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 (MCCA) )

HSP 1 Score: 1023.8 bits (2646), Expect = 6.7e-299
Identity = 519/727 (71.39%), Postives = 594/727 (81.71%), Query Frame = 0

Query: 7   RRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQTVAVFS 66
           RR +  +   + VR  S S S     + IEKIL+ANRGEIACRI+RTA+RLGIQTVAV+S
Sbjct: 10  RRNVRRKNHSMLVRYISGSASMKPKEQCIEKILVANRGEIACRIMRTAKRLGIQTVAVYS 69

Query: 67  DADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSESADFAQ 126
           DADRDSLHVKSADEAV IGP  ARLSYL+  +I++AA+RTGAQAIHPGYGFLSES+DFAQ
Sbjct: 70  DADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFAQ 129

Query: 127 LCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLEADKIGY 186
           LCED GLTFIGPP SAIR MGDKSASKRIMGAAGVPLVPGYHG  QDID MK EA+KIGY
Sbjct: 130 LCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIGY 189

Query: 187 PILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPRHIEVQI 246
           PI+IKPTHGGGGKGMRIV S  +F D+FLGAQREAAASFG++TILLEKYIT+PRHIEVQI
Sbjct: 190 PIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQI 249

Query: 247 FGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYNAGT 306
           FGD HGN+LHLYERDCSVQRRHQKIIEEAPAPN+ + FR++LGQAAVSAA+AVGYYNAGT
Sbjct: 250 FGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAGT 309

Query: 307 VEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQSQVPLLG 366
           VEFIVDT + QFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGE LP++QS+VP+ G
Sbjct: 310 VEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSG 369

Query: 367 HAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPMIAKLVV 426
           HAFEARIYAENVPKGFLPATG L+HY PV VS S+RVETGVEQGD VS+HYDPMIAKLVV
Sbjct: 370 HAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVV 429

Query: 427 WGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYKDDLFVD 486
           WG NR  ALVKLKDCL+ F+VAGVPTNINFL KLA+H  F  G+VETHFIE +K DLF D
Sbjct: 430 WGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFAD 489

Query: 487 PSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRVHHSAKR 546
            SN    + AY+A   +A L AAC+ ++E S  NE ++G  + SIWYS PPFRVHH AK+
Sbjct: 490 ESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGK-VPSIWYSNPPFRVHHEAKQ 549

Query: 547 TIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNFRVEVDG 606
           TIE EW N  + + S   +L + YQ DG +L+E G N S ++E++VT  GK +FRVE  G
Sbjct: 550 TIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEG-NDSPSLELRVTRAGKCDFRVEAAG 609

Query: 607 IIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDES-QHKPGFEAASNHPEGT 666
           + M V LA Y KD  KH+HIWHGS HH FKQ +G+  SED+E  QH+   E  S+HP GT
Sbjct: 610 LSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSE-TSSHPPGT 669

Query: 667 VVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQQVSDG 726
           +VAPMAGLVVKVLV+N  +V +GQ +LVLEAMKMEHVVKAP +G I  L V  GQQVSDG
Sbjct: 670 IVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDG 729

Query: 727 SILFCVK 733
           S LF +K
Sbjct: 730 SALFRIK 733

BLAST of Lag0002370 vs. TAIR 10
Match: AT1G03090.1 (methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 (MCCA) )

HSP 1 Score: 984.9 bits (2545), Expect = 3.4e-287
Identity = 506/727 (69.60%), Postives = 576/727 (79.23%), Query Frame = 0

Query: 7   RRKLPNRPCLLRVRLFSHSNSNIEPARPIEKILIANRGEIACRIIRTARRLGIQTVAVFS 66
           RR +  +   + VR  S S S     + IEKIL+ANRGEIACRI+RTA+RLGIQTVAV+S
Sbjct: 10  RRNVRRKNHSMLVRYISGSASMKPKEQCIEKILVANRGEIACRIMRTAKRLGIQTVAVYS 69

Query: 67  DADRDSLHVKSADEAVHIGPSPARLSYLNAASIVKAASRTGAQAIHPGYGFLSESADFAQ 126
           DADRDSLHVKSADEAV IGP  ARLSYL+  +I++AA+RTGAQAIHPGYGFLSES+DFAQ
Sbjct: 70  DADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFAQ 129

Query: 127 LCEDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKLEADKIGY 186
           LCED GLTFIGPP SAIR MGDKSASKRIMGAAGVPLVPGYHG  QDID MK EA+KIGY
Sbjct: 130 LCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIGY 189

Query: 187 PILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQPRHIEVQI 246
           PI+IKPTHGGGGKGMRIV S  +F D+FLGAQREAAASFG++TILLEKYIT+PRHIEVQI
Sbjct: 190 PIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQI 249

Query: 247 FGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYNAGT 306
           FGD HGN+LHLYERDCSVQRRHQKIIEEAPA                    AVGYYNAGT
Sbjct: 250 FGDKHGNVLHLYERDCSVQRRHQKIIEEAPA--------------------AVGYYNAGT 309

Query: 307 VEFIVDTITGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPINQSQVPLLG 366
           VEFIVDT + QFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGE LP++QS+VP+ G
Sbjct: 310 VEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSG 369

Query: 367 HAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDPMIAKLVV 426
           HAFEARIYAENVPKGFLPATG L+HY PV VS S+RVETGVEQGD VS+HYDPMIAKLVV
Sbjct: 370 HAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVV 429

Query: 427 WGENRTAALVKLKDCLTKFEVAGVPTNINFLLKLANHYAFERGDVETHFIEQYKDDLFVD 486
           WG NR  ALVKLKDCL+ F+VAGVPTNINFL KLA+H  F  G+VETHFIE +K DLF D
Sbjct: 430 WGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFAD 489

Query: 487 PSNLVMAKEAYEAAGLNARLAAACLISLELSKLNEKSYGTGIHSIWYSPPPFRVHHSAKR 546
            SN    + AY+A   +A L AAC+ ++E S  NE ++G  + SIWYS PPFRVHH AK+
Sbjct: 490 ESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGK-VPSIWYSNPPFRVHHEAKQ 549

Query: 547 TIEFEWENHYDSSVSKPFALTITYQQDGGFLVETGENGSSAVEVKVTHLGKHNFRVEVDG 606
           TIE EW N  + + S   +L + YQ DG +L+E G N S ++E++VT  GK +FRVE  G
Sbjct: 550 TIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEG-NDSPSLELRVTRAGKCDFRVEAAG 609

Query: 607 IIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQNLGVNLSEDDES-QHKPGFEAASNHPEGT 666
           + M V LA Y KD  KH+HIWHGS HH FKQ +G+  SED+E  QH+   E  S+HP GT
Sbjct: 610 LSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSE-TSSHPPGT 669

Query: 667 VVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAVGQQVSDG 726
           +VAPMAGLVVKVLV+N  +V +GQ +LVLEAMKMEHVVKAP +G I  L V  GQQVSDG
Sbjct: 670 IVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDG 713

Query: 727 SILFCVK 733
           S LF +K
Sbjct: 730 SALFRIK 713

BLAST of Lag0002370 vs. TAIR 10
Match: AT5G35360.1 (acetyl Co-enzyme a carboxylase biotin carboxylase subunit )

HSP 1 Score: 432.2 bits (1110), Expect = 8.6e-121
Identity = 220/464 (47.41%), Postives = 299/464 (64.44%), Query Frame = 0

Query: 36  EKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLN 95
           +KIL+ANRGEIA R+IRTA  +GI  VAV+S  D+D+LHVK ADEAV IG +P+  SYL 
Sbjct: 75  DKILVANRGEIAVRVIRTAHEMGIPCVAVYSTIDKDALHVKLADEAVCIGEAPSNQSYLV 134

Query: 96  AASIVKAASRTGAQAIHPGYGFLSESADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRI 155
             +++ AA   G   +HPGYGFLSE+A F ++C D G+ FIGP   +IR MGDK+ ++  
Sbjct: 135 IPNVLSAAISRGCTMLHPGYGFLSENALFVEMCRDHGINFIGPNPDSIRVMGDKATARET 194

Query: 156 MGAAGVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFL 215
           M  AGVP VPG  G  Q  +     A++IG+P++IK T GGGG+GMR+   P EFV    
Sbjct: 195 MKNAGVPTVPGSDGLLQSTEEAVRVANEIGFPVMIKATAGGGGRGMRLAKEPGEFVKLLQ 254

Query: 216 GAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEA 275
            A+ EAAA+FG     LEK++  PRHIE Q+  D  GN++H  ERDCS+QRR+QK++EEA
Sbjct: 255 QAKSEAAAAFGNDGCYLEKFVQNPRHIEFQVLADKFGNVVHFGERDCSIQRRNQKLLEEA 314

Query: 276 PAPNVLDDFRSHLGQAAVSAAKAVGYYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTE 335
           P+P +  + R  +G AAV+AA ++GY   GTVEF++D   G FYFMEMNTR+QVEHPVTE
Sbjct: 315 PSPALTAELRKAMGDAAVAAAASIGYIGVGTVEFLLDE-RGSFYFMEMNTRIQVEHPVTE 374

Query: 336 MIVGQDLVEWQIRVANGESLPINQSQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPV 395
           MI   DL+E QIRVA GE L   Q  + L GH+ E RI AE+  KGF P  G +  Y P 
Sbjct: 375 MIYSVDLIEEQIRVAMGEKLRYKQEDIVLRGHSIECRINAEDPFKGFRPGPGRITSYLP- 434

Query: 396 PVSQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNIN 455
                +R+++ V     V   YD ++ KL+VW   R  A+ ++K  L    + GVPT IN
Sbjct: 435 SGGPFVRMDSHVYSDYVVPPSYDSLLGKLIVWAPTREKAIERMKRALNDTIITGVPTTIN 494

Query: 456 FLLKLANHYAFERGDVETHFIEQYKDDLFVDPSNLVMAKEAYEA 500
           +   + +   F+ G V+T FI +++++L  +P  +V  K+   A
Sbjct: 495 YHKLILDVEDFKNGKVDTAFIVKHEEEL-AEPQEIVAVKDLTNA 535

BLAST of Lag0002370 vs. TAIR 10
Match: AT5G35360.3 (acetyl Co-enzyme a carboxylase biotin carboxylase subunit )

HSP 1 Score: 428.3 bits (1100), Expect = 1.2e-119
Identity = 216/448 (48.21%), Postives = 292/448 (65.18%), Query Frame = 0

Query: 36  EKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLN 95
           +KIL+ANRGEIA R+IRTA  +GI  VAV+S  D+D+LHVK ADEAV IG +P+  SYL 
Sbjct: 75  DKILVANRGEIAVRVIRTAHEMGIPCVAVYSTIDKDALHVKLADEAVCIGEAPSNQSYLV 134

Query: 96  AASIVKAASRTGAQAIHPGYGFLSESADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRI 155
             +++ AA   G   +HPGYGFLSE+A F ++C D G+ FIGP   +IR MGDK+ ++  
Sbjct: 135 IPNVLSAAISRGCTMLHPGYGFLSENALFVEMCRDHGINFIGPNPDSIRVMGDKATARET 194

Query: 156 MGAAGVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFL 215
           M  AGVP VPG  G  Q  +     A++IG+P++IK T GGGG+GMR+   P EFV    
Sbjct: 195 MKNAGVPTVPGSDGLLQSTEEAVRVANEIGFPVMIKATAGGGGRGMRLAKEPGEFVKLLQ 254

Query: 216 GAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEA 275
            A+ EAAA+FG     LEK++  PRHIE Q+  D  GN++H  ERDCS+QRR+QK++EEA
Sbjct: 255 QAKSEAAAAFGNDGCYLEKFVQNPRHIEFQVLADKFGNVVHFGERDCSIQRRNQKLLEEA 314

Query: 276 PAPNVLDDFRSHLGQAAVSAAKAVGYYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTE 335
           P+P +  + R  +G AAV+AA ++GY   GTVEF++D   G FYFMEMNTR+QVEHPVTE
Sbjct: 315 PSPALTAELRKAMGDAAVAAAASIGYIGVGTVEFLLDE-RGSFYFMEMNTRIQVEHPVTE 374

Query: 336 MIVGQDLVEWQIRVANGESLPINQSQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPV 395
           MI   DL+E QIRVA GE L   Q  + L GH+ E RI AE+  KGF P  G +  Y P 
Sbjct: 375 MIYSVDLIEEQIRVAMGEKLRYKQEDIVLRGHSIECRINAEDPFKGFRPGPGRITSYLP- 434

Query: 396 PVSQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRTAALVKLKDCLTKFEVAGVPTNIN 455
                +R+++ V     V   YD ++ KL+VW   R  A+ ++K  L    + GVPT IN
Sbjct: 435 SGGPFVRMDSHVYSDYVVPPSYDSLLGKLIVWAPTREKAIERMKRALNDTIITGVPTTIN 494

Query: 456 FLLKLANHYAFERGDVETHFIEQYKDDL 484
           +   + +   F+ G V+T FI +++++L
Sbjct: 495 YHKLILDVEDFKNGKVDTAFIVKHEEEL 520

BLAST of Lag0002370 vs. TAIR 10
Match: AT5G35360.2 (acetyl Co-enzyme a carboxylase biotin carboxylase subunit )

HSP 1 Score: 408.3 bits (1048), Expect = 1.3e-113
Identity = 205/418 (49.04%), Postives = 273/418 (65.31%), Query Frame = 0

Query: 36  EKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLN 95
           +KIL+ANRGEIA R+IRTA  +GI  VAV+S  D+D+LHVK ADEAV IG +P+  SYL 
Sbjct: 75  DKILVANRGEIAVRVIRTAHEMGIPCVAVYSTIDKDALHVKLADEAVCIGEAPSNQSYLV 134

Query: 96  AASIVKAASRTGAQAIHPGYGFLSESADFAQLCEDEGLTFIGPPISAIRHMGDKSASKRI 155
             +++ AA   G   +HPGYGFLSE+A F ++C D G+ FIGP   +IR MGDK+ ++  
Sbjct: 135 IPNVLSAAISRGCTMLHPGYGFLSENALFVEMCRDHGINFIGPNPDSIRVMGDKATARET 194

Query: 156 MGAAGVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFL 215
           M  AGVP VPG  G  Q  +     A++IG+P++IK T GGGG+GMR+   P EFV    
Sbjct: 195 MKNAGVPTVPGSDGLLQSTEEAVRVANEIGFPVMIKATAGGGGRGMRLAKEPGEFVKLLQ 254

Query: 216 GAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEA 275
            A+ EAAA+FG     LEK++  PRHIE Q+  D  GN++H  ERDCS+QRR+QK++EEA
Sbjct: 255 QAKSEAAAAFGNDGCYLEKFVQNPRHIEFQVLADKFGNVVHFGERDCSIQRRNQKLLEEA 314

Query: 276 PAPNVLDDFRSHLGQAAVSAAKAVGYYNAGTVEFIVDTITGQFYFMEMNTRLQVEHPVTE 335
           P+P +  + R  +G AAV+AA ++GY   GTVEF++D   G FYFMEMNTR+QVEHPVTE
Sbjct: 315 PSPALTAELRKAMGDAAVAAAASIGYIGVGTVEFLLDE-RGSFYFMEMNTRIQVEHPVTE 374

Query: 336 MIVGQDLVEWQIRVANGESLPINQSQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPV 395
           MI   DL+E QIRVA GE L   Q  + L GH+ E RI AE+  KGF P  G +  Y P 
Sbjct: 375 MIYSVDLIEEQIRVAMGEKLRYKQEDIVLRGHSIECRINAEDPFKGFRPGPGRITSYLP- 434

Query: 396 PVSQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRTAALVKLKDCLTKFEVAGVPTN 454
                +R+++ V     V   YD ++ KL+VW   R  A+ ++K  L    + G+ +N
Sbjct: 435 SGGPFVRMDSHVYSDYVVPPSYDSLLGKLIVWAPTREKAIERMKRALNDTIITGMGSN 490