Lag0002312 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002312
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	hyoscyamine 6-dioxygenase-like
Location	chr4: 41554897 .. 41556075 (-)
RNA-Seq Expression	Lag0002312
Synteny	Lag0002312

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAGGTAAATATTTTGTCCAACTGTGTGGGTGTTGAATCAGTGCCGGAAAGCTATGTGTATCCGCCGGAGATGAGGCTCGGCGTTGCGGTGAAGAAGGAAATTCCGGTGGTTGATTTTACGTCCCACGACCGAGCCCTTCTCGTTCGAGAAATCCTCGACGTTTCTCAAGAGTTCGGATTTTTTCAGGTTAGGAAGTACTCAAAAATATATAACCATGTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGATACATAACCATGTTATGTTGAAAATTATATTCTATCAACAAATATGTTATAAAAAAACTAAAAAAAATTAATTAAAAAAATGTGTCACATTAGCTTTCTAGCTTTTTTTTTTTTCTTTGAAAATATGAGGGGTGGGGATTTGAACCACAGACCTTGTGGTTATCAGTACAACTTTATGCAAGTTGAGCTATGCTCTTGTTGGCCACATTAGCTTTCTAGCTATGATAACGGAAAAAGACCAAAAAAATGCATTTTTCCCAAACCTTAAGGTTAACCTATCGAGGGACAAAATATTCAAGAATAAAATTGTCAAGTTTGACTTTTTTAATAAAATAACGAAAAATGGATGGAGGACAAAATTAAACCAAATGAAAACCTTAGGGATATAAAATATTTTAGTTTGAAAACTCAGGGACTCAAATGCAACCTATCTCAAACATCAGATACCAAAACATTGTATTCTTTAAATTTAAAAAAAAGTGTCACATCAGCTTTCTAGCTATGAGGTAAGAAAAGAGGACCAAAAAAATGCATTTTCCCAAACCTTAAGGTTAACCCGTCGAGTTTGAAAATTTAAGAACAAAAAATATGATATCAAACTTAAACCTCAAGAACAAAAGTGTAATTTTTTTTTTCTCGTCCTGATAGGTGATAAACCATGGAGTGTCAAGAAGGTTATTAGAAGAGACTATGAAGATTTTCAAGGAGTTTCATAGCATGTCAACAGCAGACAAAGTGAGGGAATGCTCAAAAGATCCAAACCAAAGCTGCAGAGTTTACATTTACACAAGCAATGAGGATTGTGTAAATGAACACATTCACTCTTGGAGAGATTATGTGCTTTTCAATTGCTATCCTTTGGAGAAATATGTTCATTTTTGGCCTCAAAATCCCCCTAAATACAGGTAA

mRNA sequence

ATGGGAGAGGTAAATATTTTGTCCAACTGTGTGGGTGTTGAATCAGTGCCGGAAAGCTATGTGTATCCGCCGGAGATGAGGCTCGGCGTTGCGGTGAAGAAGGAAATTCCGGTGGTTGATTTTACGTCCCACGACCGAGCCCTTCTCGTTCGAGAAATCCTCGACGTTTCTCAAGAGTTCGGATTTTTTCAGGTGATAAACCATGGAGTGTCAAGAAGGTTATTAGAAGAGACTATGAAGATTTTCAAGGAGTTTCATAGCATGTCAACAGCAGACAAAGTGAGGGAATGCTCAAAAGATCCAAACCAAAGCTGCAGAGTTTACATTTACACAAGCAATGAGGATTGTGTAAATGAACACATTCACTCTTGGAGAGATTATGTGCTTTTCAATTGCTATCCTTTGGAGAAATATGTTCATTTTTGGCCTCAAAATCCCCCTAAATACAGGTAA

Coding sequence (CDS)

ATGGGAGAGGTAAATATTTTGTCCAACTGTGTGGGTGTTGAATCAGTGCCGGAAAGCTATGTGTATCCGCCGGAGATGAGGCTCGGCGTTGCGGTGAAGAAGGAAATTCCGGTGGTTGATTTTACGTCCCACGACCGAGCCCTTCTCGTTCGAGAAATCCTCGACGTTTCTCAAGAGTTCGGATTTTTTCAGGTGATAAACCATGGAGTGTCAAGAAGGTTATTAGAAGAGACTATGAAGATTTTCAAGGAGTTTCATAGCATGTCAACAGCAGACAAAGTGAGGGAATGCTCAAAAGATCCAAACCAAAGCTGCAGAGTTTACATTTACACAAGCAATGAGGATTGTGTAAATGAACACATTCACTCTTGGAGAGATTATGTGCTTTTCAATTGCTATCCTTTGGAGAAATATGTTCATTTTTGGCCTCAAAATCCCCCTAAATACAGGTAA

Protein sequence

MGEVNILSNCVGVESVPESYVYPPEMRLGVAVKKEIPVVDFTSHDRALLVREILDVSQEFGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCVNEHIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR

Homology

[Close]

BLAST of Lag0002312 vs. NCBI nr
Match: XP_022143610.1 (hyoscyamine 6-dioxygenase-like [Momordica charantia])

HSP 1 Score: 217.2 bits (552), Expect = 9.4e-53
Identity = 104/151 (68.87%), Postives = 122/151 (80.79%), Query Frame = 0


Query: 3   EVNILSNCVGVESVPESYVYPPEMRLG---VAVKKEIPVVDFTSHDRALLVREILDVSQE 62
           +VN +SN VGVESVPESYVYPPE R G   V V+  IPVVD T HDRA LV +ILD SQE
Sbjct: 4   QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63

Query: 63  FGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCVNE 122
           FGFFQVINHGVS+ L+EETM++F+EFH+MS  +K RECSKDPN+SCRV  YTS+E+ V E
Sbjct: 64  FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRV--YTSSENFVKE 123

Query: 123 HIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR 151
             H WRD ++FNC+PL+KYVHFWPQNP  YR
Sbjct: 124 KTHCWRDALIFNCHPLDKYVHFWPQNPSNYR 152

[Close]

BLAST of Lag0002312 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 216.1 bits (549), Expect = 2.1e-52
Identity = 103/153 (67.32%), Postives = 124/153 (81.05%), Query Frame = 0


Query: 1   MGEVNILSNCVGVESVPESYVYPPEMRLG---VAVKKEIPVVDFTSHDRALLVREILDVS 60
           MGEV  +SN  GV+SVP  YVYPPE R G   V +   IPV+D ++HDR LLVR+ILD S
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCV 120
           QEFGFFQVINHGVSRR+LEET +IFK+FH+MS A+KV+ECS+DPN+SCRV  YTS+E+  
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRV--YTSSENYR 120

Query: 121 NEHIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR 151
            E  H WRD ++FNC+PL+KYVHFWPQNPPKYR
Sbjct: 121 KEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYR 151

[Close]

BLAST of Lag0002312 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.8e-51
Identity = 102/153 (66.67%), Postives = 123/153 (80.39%), Query Frame = 0


Query: 1   MGEVNILSNCVGVESVPESYVYPPEMRLG---VAVKKEIPVVDFTSHDRALLVREILDVS 60
           MGEV  +SN  GV+SVP  YVYPPE R G   V +   IPV+D ++ DR LLVR+ILD S
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCV 120
           QEFGFFQVINHGVSRR+LEET +IFK+FH+MS A+KV+ECS+DPN+SCRV  YTS+E+  
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRV--YTSSENYR 120

Query: 121 NEHIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR 151
            E  H WRD ++FNC+PL+KYVHFWPQNPPKYR
Sbjct: 121 KEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYR 151

[Close]

BLAST of Lag0002312 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210.3 bits (534), Expect = 1.1e-50
Identity = 100/153 (65.36%), Postives = 122/153 (79.74%), Query Frame = 0


Query: 1   MGEVNILSNCVGVESVPESYVYPPEMRLG---VAVKKEIPVVDFTSHDRALLVREILDVS 60
           MGEV  +SN  GV+SVP  YVYPPE R G   V +   IPV+D ++ DR LLVR+ILD S
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCV 120
           QEFGFFQVINHGVS+R+LEET +IFK+FH+MS A+K +ECS+DPN+SCRV  YTS+E+  
Sbjct: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRV--YTSSENYR 120

Query: 121 NEHIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR 151
            E  H WRD ++FNC+PL+KYVHFWPQNPPKYR
Sbjct: 121 KEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYR 151

[Close]

BLAST of Lag0002312 vs. NCBI nr
Match: XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 210.3 bits (534), Expect = 1.1e-50
Identity = 101/153 (66.01%), Postives = 121/153 (79.08%), Query Frame = 0


Query: 1   MGEVNILSNCVGVESVPESYVYPPEMRLG---VAVKKEIPVVDFTSHDRALLVREILDVS 60
           MGEV  +SN  GV+SVP  Y+YPPE R G   V +   IPVVD ++ DR LLVR+ILD S
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCV 120
           QEFGFFQVINHGVSRR+LEET +IFK+FH+MS A+K +ECS+DP +SCRV  YTS+E+  
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRV--YTSSENYR 120

Query: 121 NEHIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR 151
            E  H WRD ++FNC+PLEKYVHFWPQNPPKYR
Sbjct: 121 KEQTHCWRDALIFNCHPLEKYVHFWPQNPPKYR 151

[Close]

BLAST of Lag0002312 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.0e-25
Identity = 52/139 (37.41%), Postives = 93/139 (66.91%), Query Frame = 0


Query: 15  SVPESYVYPPEMR---LGVAVKKEIPVVDFTSHDRALLVREILDVSQEFGFFQVINHGVS 74
           ++PE+YV P   R     V+  ++ P++D +S DR+ L+++I      FGFFQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  RRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCVNEHIHSWRDYVLFN 134
           +++++E + + +EF SMS  +K++  S DP ++ R+   +++ +   E +++WRDY+  +
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL---STSFNVKKEEVNNWRDYLRLH 133

Query: 135 CYPLEKYVHFWPQNPPKYR 151
           CYP+ KYV+ WP NPP ++
Sbjct: 134 CYPIHKYVNEWPSNPPSFK 149

[Close]

BLAST of Lag0002312 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 5.8e-21
Identity = 51/140 (36.43%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 14  ESVPESYVYPPEM--RLGVAVK-KEIPVVDFTSHDRALLVREILDVSQEFGFFQVINHGV 73
           +++P  YV P     RL + V    IPVVD  S DRA +V  + D  +  GFFQV+NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 74  SRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCVNEHIHSWRDYVLF 133
              L+   M++ +EF  +   +K +  S DP +  R+   +++ +   E +H+WRDY+  
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRL---STSFNVRKETVHNWRDYLRL 137

Query: 134 NCYPLEKYVHFWPQNPPKYR 151
           +CYPL ++V  WP NPP ++
Sbjct: 138 HCYPLHQFVPDWPSNPPSFK 154

[Close]

BLAST of Lag0002312 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 8.6e-17
Identity = 51/146 (34.93%), Postives = 80/146 (54.79%), Query Frame = 0


Query: 14  ESVPESYVYPPEMRL--GVAVKKEIPVVDFTSHDRALLVREILDVSQEFGFFQVINHGVS 73
           +SV ES++ P + R    V V  ++P++D   H   LLV++I    Q+FG FQVINHG  
Sbjct: 11  KSVSESFIAPLQKRAEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDFGLFQVINHGFP 70

Query: 74  RRLLEETMKIFKEFHSMSTADKVR-----ECSK-DPNQSCRVYIYTSNEDCVNEHIHSWR 133
             L+ ETM++ KEF ++   +K +     E +K +     +  +Y   E   NE    W+
Sbjct: 71  EELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLSNEEFLYWK 130

Query: 134 DYVLFNCYPLEK-YVHFWPQNPPKYR 151
           D +   C+PL++  V+ WP+ P KYR
Sbjct: 131 DTLAHGCHPLDQDLVNSWPEKPAKYR 155

[Close]

BLAST of Lag0002312 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.2e-14
Identity = 43/147 (29.25%), Postives = 77/147 (52.38%), Query Frame = 0


Query: 13  VESVPESYVYP----PEMRLGVAVKKEIPVVDFTSHD-----RALLVREILDVSQEFGFF 72
           V+ VP +YV P    P+M         IP++D   HD     RA ++ +        GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCVNEHIHS 132
           Q+ NHGV    +++ M   +EF   S +++V+  S D  ++ R+   +++ +   E + +
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRL---STSFNVSKEKVSN 134

Query: 133 WRDYVLFNCYPLEKYVHFWPQNPPKYR 151
           WRD++  +CYP+E +++ WP  P  +R
Sbjct: 135 WRDFLRLHCYPIEDFINEWPSTPISFR 156

[Close]

BLAST of Lag0002312 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 5.8e-13
Identity = 37/152 (24.34%), Postives = 81/152 (53.29%), Query Frame = 0


Query: 6   ILSNCVGVESVPESYVYP----PEMRLGVAVKKEIPVVDFT---SHDRALLVREILDVSQ 65
           ++S+      +P +YV P    P +    +    IP++D       +RA++V+++     
Sbjct: 10  LVSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACS 69

Query: 66  EFGFFQVINHGVSRRLLEETMKIFKEFHSMSTADKVRECSKDPNQSCRVYIYTSNEDCVN 125
            +GFFQ+ NHGV    + +   + +EF     +++V+  S DP ++ R+   +++ +   
Sbjct: 70  TYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRL---STSFNVGA 129

Query: 126 EHIHSWRDYVLFNCYPLEKYVHFWPQNPPKYR 151
           + + +WRD++  +C+P+E ++  WP +P  +R
Sbjct: 130 DKVLNWRDFLRLHCFPIEDFIEEWPSSPISFR 158

The following BLAST results are available for this feature:

BLAST of Lag0002312 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143610.1	9.4e-53	68.87	hyoscyamine 6-dioxygenase-like [Momordica charantia]	[more]
XP_022959666.1	2.1e-52	67.32	hyoscyamine 6-dioxygenase-like [Cucurbita moschata]	[more]
KAG6593078.1	1.8e-51	66.67	Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023514953.1	1.1e-50	65.36	hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004374.1	1.1e-50	66.01	hyoscyamine 6-dioxygenase-like [Cucurbita maxima]	[more]

BLAST of Lag0002312 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLV0	5.0e-25	37.41	Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S...	[more]
Q8W2X5	5.8e-21	36.43	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]
P24397	8.6e-17	34.93	Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1	[more]
Q9ZSA7	5.2e-14	29.25	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q9ZSA8	5.8e-13	24.34	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 36..144 e-value: 3.4E-16 score: 60.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..150 e-value: 4.5E-23 score: 84.3
None	No IPR available	PANTHER	PTHR47991:SF31	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 9..150
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 9..150
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 11..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002312.1	Lag0002312.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding