Lag0002125 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002125 vs. NCBI nr
Match: RVW70235.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 1013.4 bits (2619), Expect = 1.5e-291
Identity = 496/1127 (44.01%), Postives = 701/1127 (62.20%), Query Frame = 0

Query: 1    MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLS 60
            MK+ISWN RGLGS+ KR +VKDFL  E PD+V+ QETK +  DRR V SVW++R+  W +
Sbjct: 812  MKIISWNTRGLGSKKKRRVVKDFLRSEKPDVVMFQETKKEECDRRFVGSVWTARNKDWAA 871

Query: 61   LDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSF 120
            L A G++GGIL++W    +   + +LG++S+S + T +G    W++ VYGP +S+ RK  
Sbjct: 872  LPACGASGGILIIWDTKKLSREEVMLGSFSVSIKFTLNGCESLWLSAVYGPNNSALRKDL 931

Query: 121  LQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHG 180
              EL+D+AGL    WCV GDFN++R   E+L  +R T SM+ F+ FIS  +LID+P+   
Sbjct: 932  WVELSDIAGLASPRWCVGGDFNVIRRSSEKLGGSRLTPSMKDFDDFISDCELIDLPLRSA 991

Query: 181  RFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMP 240
             FTWS +       RLDRFL +++W  TF +     L R TSDH+P+ L     +WGP P
Sbjct: 992  SFTWSNMQVNPVCKRLDRFLYSNEWEQTFPQSIQGVLPRWTSDHWPIVLETNPFKWGPTP 1051

Query: 241  FRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKK 300
            FRFENMWL HP F+++  +WW EF   GW G +FM KL+ +K +LK WNK  FG L+++K
Sbjct: 1052 FRFENMWLQHPSFKENFGRWWREFQGNGWEGHKFMRKLQFVKAKLKVWNKASFGELSKRK 1111

Query: 301  KTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDEN 360
            + IL  +   D  E++G +    +A+R   K  L E+ + ++    QK ++KW++EGD N
Sbjct: 1112 EDILSALVNFDSLEQEGGLSHELLAQRAIKKGELEELILREEIHWRQKARVKWVKEGDCN 1171

Query: 361  SAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPN 420
            S FFH+     +++ FI  LE+E G+ ++    I++EI+ +F  LY+      + ++G +
Sbjct: 1172 SKFFHKVANGRRNRKFIKELENENGQMMNNSESIKEEILRYFEKLYTSPSGESWRVEGLD 1231

Query: 421  WAPLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQ 480
            W+P+  + + RLE PF E+EI +AI  +   K+PGPDG T   F++ W ++K DLV+VF 
Sbjct: 1232 WSPISGESAVRLESPFTEEEICKAIFQMDRDKAPGPDGFTIAVFQDCWEVIKEDLVKVFT 1291

Query: 481  EFFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTT 540
            EF R+GIIN+ TN ++I L+PKK  + +ISDFRPISL+TSLYKI+AKVLA R++++L  T
Sbjct: 1292 EFHRSGIINQSTNASFIVLLPKKSMSRRISDFRPISLITSLYKIIAKVLAGRIREVLHET 1351

Query: 541  ISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKL 600
            I   Q AFVQGR ILD +L+A+E+V+E R   E+GV+FK+DFEKAYD VSW+FLD ++++
Sbjct: 1352 IHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFLDHVMEM 1411

Query: 601  KGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSA 660
            KGFG  WRKW+RGCL++ +F++++NG  +G V ASRGLRQGDPLSPFLFTIV D LSR  
Sbjct: 1412 KGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRML 1471

Query: 661  HYCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLN 720
                E+ VL+G  VGR++T VS LQ+ADDTI FS+  E  M+    +L++    SGL +N
Sbjct: 1472 LKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFSSSREEDMMTLKNVLLVFGHISGLKVN 1531

Query: 721  LAKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKL 780
            L K++I GIN     ++R A    CK    PI  LG PLGGN +T  FWDP++++   +L
Sbjct: 1532 LDKSNIYGINLEQNHLSRLAEMLDCKASGWPILYLGLPLGGNPKTSGFWDPVIERISRRL 1591

Query: 781  EGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPAC 840
            +GW+K  LS GGR+TL QS L  +P Y+ SL K P  V  K+E++ RDF+W+G       
Sbjct: 1592 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEGKRD 1651

Query: 841  NLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGW 900
            +LV W+    P S GG+G G +  RN ALL KWLWR+  E   LW +VI SIYG  S GW
Sbjct: 1652 HLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNGW 1711

Query: 901  RTMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYE 960
                I     R  W  I   Y  F KFT+F V  G RIRFW D W   QPL V +P +  
Sbjct: 1712 DVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPRLLR 1771

Query: 961  ISHMKLASVAECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWK 1020
            +   K A ++         +WN   RR L D E+     L+   D + + + + D+ SW 
Sbjct: 1772 VVTDKNAPISSILGSTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKRSWS 1831

Query: 1021 LEGSGLFSTKSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQR 1080
            L  SGLF+ KS F A    S    +     +W  + P KVK F+W VA++ +NT+D +Q 
Sbjct: 1832 LSSSGLFTVKSFFLALSQYSVSPPIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDLLQL 1891

Query: 1081 KLKNWALSPRDVDYVLKR----VRILITSSFIVSMLARLGTSLQVCW 1122
            +    ALSP      +K       + +  S  + +  RL  S ++ W
Sbjct: 1892 RRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDW 1938

BLAST of Lag0002125 vs. NCBI nr
Match: RVW64408.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 1003.4 bits (2593), Expect = 1.5e-288
Identity = 492/1090 (45.14%), Postives = 682/1090 (62.57%), Query Frame = 0

Query: 1    MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLS 60
            MK++SWN RGLGS+ KR +V+ FLS +NPD+V+LQETK +  DRR V SVW  + V W +
Sbjct: 736  MKILSWNTRGLGSKKKRRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKGKRVEWAA 795

Query: 61   LDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSF 120
            L A G++GGI+++W  + +E  + VLG++S++ +     +   W+T VYGP +   RK F
Sbjct: 796  LPACGASGGIVILWDSSKLECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDF 855

Query: 121  LQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHG 180
              EL D+ GL    WCV GDFN++R + E+L  TR T +MR F+ FI    LID P+ + 
Sbjct: 856  WLELQDLYGLTFPRWCVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLIDPPLRNA 915

Query: 181  RFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMP 240
             FTWS +       RLDRFL + +W   F +   + L R TSDH P+ L    ++WGP P
Sbjct: 916  AFTWSNMQADPICKRLDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTP 975

Query: 241  FRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKK 300
            FRFENMWL HP+F++    WW E T  GW G +FM KLK +K +LKEWN   FG+L E+K
Sbjct: 976  FRFENMWLLHPEFKEKFRVWWLECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERK 1035

Query: 301  KTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDEN 360
            K IL  +  +D  E++G++    + ER   +  L ++ + ++ +  QK ++KW++EGD N
Sbjct: 1036 KLILTDLSRIDLIEQEGNLNSDLVLERTLKRRELEDVLLKEEVQWRQKSRVKWIKEGDCN 1095

Query: 361  SAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPN 420
            S FFHR     +S+ FI +L SE GE L+   DI +EI+ FF NLYSK     + ++G +
Sbjct: 1096 SKFFHRVATGRRSRKFIKSLISERGETLNNIEDISEEIVNFFGNLYSKPVGESWRVEGID 1155

Query: 421  WAPLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQ 480
            W P+  +    L+ PF E+E+ RA+  L   K+PGPDG T   ++  W+++K DL+ VF 
Sbjct: 1156 WVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAPGPDGFTIAVYQECWDVIKEDLMRVFL 1215

Query: 481  EFFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTT 540
            EF  NG+IN+ TN T+I L+PKK  + KISD+RPISLVTSLYKI+AKVL+ RL+ +L  T
Sbjct: 1216 EFHTNGVINQSTNATFIALVPKKSQSVKISDYRPISLVTSLYKIIAKVLSGRLRKVLHET 1275

Query: 541  ISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKL 600
            ISD Q AFV+GR ILD +L+A+EVV+E R   E+G++FK+DFEKAYD V W FLD +L+ 
Sbjct: 1276 ISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQR 1335

Query: 601  KGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSA 660
            KGF   WR WIRGCL++S+F+I++NG  +G V ASRGLRQGDPLSPFLFT+V D LSR  
Sbjct: 1336 KGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKASRGLRQGDPLSPFLFTLVADVLSRML 1395

Query: 661  HYCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLN 720
                E  + +G  VGRD+T VS+LQ+ADDTI FS      + N   IL++  + SGL +N
Sbjct: 1396 FRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFSKASMEHLQNLKIILLVFGQVSGLKIN 1455

Query: 721  LAKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKL 780
            L K++I GINT  E ++  A+ F C+V   P+  LG PLGGN +T  FWDP+V++   +L
Sbjct: 1456 LEKSTISGINTRQELLSSLASVFDCRVSEWPLSYLGLPLGGNPKTIGFWDPVVERISRRL 1515

Query: 781  EGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPAC 840
            +GWKK  LS GGR+TL QS L+ +P Y+ SL K P  +  K+EK+ R+F+W+G       
Sbjct: 1516 DGWKKAYLSLGGRITLIQSCLSHIPSYFLSLFKIPASIASKIEKMQRNFLWSGAGEGKKD 1575

Query: 841  NLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGW 900
            +LV+WE  + P   GG+G G +  RN ALL KWLWRF  E+ GLW +VIGSIYG    GW
Sbjct: 1576 HLVRWEVVSRPKELGGLGFGKISLRNIALLGKWLWRFPRERSGLWYKVIGSIYGTHPNGW 1635

Query: 901  RTMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYE 960
               ++     R  W  I + +  F  F +  V  G RIRFW D W   Q L   F ++Y 
Sbjct: 1636 DANMVVRWSHRCPWKAIAQVFQEFSPFVRLVVGNGERIRFWEDLWWGNQSLCSQFADLYR 1695

Query: 961  ISHMKLASVAECWDEN-NQTWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWK 1020
            +  +K  +V+     +    WNL  RR L D E+     L+  L +++    + D  +W 
Sbjct: 1696 VISVKNLTVSNVLGNSFPLAWNLNFRRNLTDSEIDLLQRLMSSLSSVRFFPSLADSRAWS 1755

Query: 1021 LEGSGLFSTKSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQR 1080
            L  SGLF+ KS F A    S  I    A  +W  K P KVK   W VA+  +NT+DK+Q 
Sbjct: 1756 LSSSGLFTVKSFFLALSKVSNPILFLPAKFLWSSKVPSKVKALAWIVAHGKVNTNDKLQL 1815

Query: 1081 KLKNWALSPR 1089
            +    +L P+
Sbjct: 1816 RRPYKSLCPQ 1825

BLAST of Lag0002125 vs. NCBI nr
Match: CAN68165.1 (hypothetical protein VITISV_008538 [Vitis vinifera])

HSP 1 Score: 982.2 bits (2538), Expect = 3.7e-282
Identity = 483/1088 (44.39%), Postives = 672/1088 (61.76%), Query Frame = 0

Query: 2    KLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLSL 61
            K+ISWN RGLGSR KR +VKDFL  E PD+V++QETK    DRR V SVW++R+  W  L
Sbjct: 113  KIISWNTRGLGSRKKRRVVKDFLRSEKPDIVMIQETKKAECDRRFVGSVWTARNKEWAVL 172

Query: 62   DAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSFL 121
             A G++GGIL++W    +   + VLG++S+S +    G  Q W++ VYGP S++ RK F 
Sbjct: 173  PACGASGGILVIWDSKKLHSEEVVLGSFSVSVKFAVDGSEQFWJSAVYGPNSTALRKDFW 232

Query: 122  QELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHGR 181
             EL+D+ GL    WCV GDFN++R   E+L   R T SM+  + FI   +LID P+    
Sbjct: 233  VELSDIFGLSSPCWCVGGDFNVIRRCSEKLGGGRLTPSMKDLDDFIRENELIDPPLRSAS 292

Query: 182  FTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMPF 241
            FTWS + E     RLDRFL +++W   F +   + L R TSDH+P+ L     +WGP PF
Sbjct: 293  FTWSNMQEHPVCKRLDRFLYSNEWEQLFPQSLQEVLPRWTSDHWPIVLETNPFKWGPTPF 352

Query: 242  RFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKKK 301
            RFENMWL HP F++   +WW EF   GW G +FM KL+ LK +LKEWNK  FG+L E+KK
Sbjct: 353  RFENMWLHHPSFKECFGRWWREFQGDGWEGHKFMRKLQFLKAKLKEWNKNAFGDLIERKK 412

Query: 302  TILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDENS 361
             IL  I   D  E++G + P  + +R   K  L E+ + ++    QK ++KW++EGD NS
Sbjct: 413  CILLDIANFDSMEQEGGLSPELLIQRAVRKGELEELILREEIHWRQKARVKWVKEGDCNS 472

Query: 362  AFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPNW 421
              FH+     +++ FI  LE+E G  L     I++EI+ +F  LY+      + ++G +W
Sbjct: 473  KXFHKVANGRRNRKFIKVLENERGLVLDNSDSIKEEILRYFEKLYASPSGESWRVEGLDW 532

Query: 422  APLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQE 481
            +P+  + ++RLE PF E+EI++AI  +    +PGPDG T   F++ W+++K DLV VF E
Sbjct: 533  SPISRESASRLESPFTEEEIYKAIFQMDRDXAPGPDGFTIAVFQDCWDVIKEDLVRVFDE 592

Query: 482  FFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTI 541
            F R+GIIN+ TN ++I L+PKK  A KIS++RPISL+TSLYKI+AKVLA RL+ IL  TI
Sbjct: 593  FHRSGIINQSTNASFIVLLPKKSMAKKISNYRPISLITSLYKIIAKVLAGRLRGILHETI 652

Query: 542  SDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLK 601
               Q AFVQGR ILD +L+A+E+V+E +   E+GV+FK+DFEKAYD VSW+FLD +++ K
Sbjct: 653  HSTQGAFVQGRQILDAVLIANEIVDEKKRSGEEGVVFKIDFEKAYDHVSWDFLDHVMEKK 712

Query: 602  GFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAH 661
            GF    RKWIR CL++ +F+I++NG  +G V   RGLRQGDPLSPFLFTIV D  S    
Sbjct: 713  GFNPXXRKWIRXCLSSVSFAILVNGNAKGWVKXXRGLRQGDPLSPFLFTIVADVXSXMLL 772

Query: 662  YCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLNL 721
               E+ V +G  VGR++T VS LQ+ADDTI FS+  E  +L    +L +    SGL +NL
Sbjct: 773  RAEERNVFEGFRVGRNRTRVSHLQFADDTIFFSSTREEDLLTLKSVLXVFGHISGLKVNL 832

Query: 722  AKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKLE 781
             K++I GIN   + + R A    CK    PI  LG PLGGN ++ +FWDP++++  ++L+
Sbjct: 833  DKSNIYGINLGQDHLHRLAELLDCKASGWPILYLGLPLGGNPKSGSFWDPVIERISSRLD 892

Query: 782  GWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPACN 841
            GW+K  LS GGR+TL QS L  +P Y+ SL K P  V  ++E+L RDF+W+G       +
Sbjct: 893  GWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAGRIERLQRDFLWSGVGEGKRDH 952

Query: 842  LVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGWR 901
            LV W+        GG+G+G +  RN+ALL KWLWR+  E   LW +VI SIYG  S GW 
Sbjct: 953  LVSWBVVCKSKMKGGLGLGRISLRNSALLGKWLWRYPREGSALWHQVILSIYGSHSNGWD 1012

Query: 902  TMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYEI 961
                     R  W  I + +  F KFT+F V  G RIRFW D W   Q L V FP +  +
Sbjct: 1013 ANTXVRWSHRCPWKAIAQVFQDFSKFTRFIVGDGDRIRFWEDLWWGDQSLGVRFPRLLRV 1072

Query: 962  SHMKLASVAECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWKL 1021
               K   ++         +WN   RR L D E+    +L+  LD+I +   + D+ SW L
Sbjct: 1073 VMDKNILISSILGSTRPFSWNFNFRRNLSDSEIEKVESLMQSLDHIHLSPSVPDKRSWSL 1132

Query: 1022 EGSGLFSTKSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQRK 1081
              SGLF+ KS F A    S   ++     +W  + P K+K F+W VA++ +NT+D +Q +
Sbjct: 1133 SSSGLFTVKSFFLALSQISGLPSVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTNDMLQLR 1192

Query: 1082 LKNWALSP 1088
                ALSP
Sbjct: 1193 RPYKALSP 1200

BLAST of Lag0002125 vs. NCBI nr
Match: CAN68838.1 (hypothetical protein VITISV_030956 [Vitis vinifera])

HSP 1 Score: 976.9 bits (2524), Expect = 1.5e-280
Identity = 470/1040 (45.19%), Postives = 658/1040 (63.27%), Query Frame = 0

Query: 1    MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLS 60
            MK+ISWN RGLGS+ KR +VKDFL  E PD+V+ QETK +  DRR V SVW++R+  W +
Sbjct: 830  MKIISWNTRGLGSKKKRRVVKDFLRSEKPDVVMFQETKKEECDRRFVGSVWTARNKDWAA 889

Query: 61   LDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSF 120
            L A G++GGIL++W    +   + +LG++S+S + T +G    W++ VYGP +S+ RK  
Sbjct: 890  LPACGASGGILIIWDTKKLSREEVMLGSFSVSIKFTLNGCESLWLSAVYGPNNSALRKDL 949

Query: 121  LQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHG 180
              EL+D+AGL    WCV GDFN++R   E+L  +R T SM+ F+ FIS  +LID+P+   
Sbjct: 950  WVELSDIAGLASPRWCVGGDFNVIRRSSEKLGGSRLTPSMKDFDDFISDCELIDLPLRSA 1009

Query: 181  RFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMP 240
             FTWS +       RLDRFL +++W  TF +     L R TSDH+P+ L     +WGP P
Sbjct: 1010 SFTWSNMQVNPVCKRLDRFLYSNEWEQTFPQSIQGVLPRWTSDHWPIVLETNPFKWGPTP 1069

Query: 241  FRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKK 300
            FRFENMWL HP F+++  +WW EF   GW G +FM KL+ +K +LK WNK  FG L+++K
Sbjct: 1070 FRFENMWLQHPSFKENFGRWWREFQGNGWEGHKFMRKLQFVKAKLKVWNKASFGELSKRK 1129

Query: 301  KTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDEN 360
            + IL  +   D  E++G +    +A+R   K  L E+ + ++    QK ++KW++EGD N
Sbjct: 1130 EDILSALVNFDSLEQEGGLSHELLAQRAIKKGELEELILREEIHWRQKARVKWVKEGDCN 1189

Query: 361  SAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPN 420
            S FFH+     +++ FI  LE+E G+ ++    I++EI+ +F  LY+      + ++G +
Sbjct: 1190 SKFFHKVANGRRNRKFIKELENENGQMMNNSESIKEEILRYFEKLYTSPSGESWRVEGLD 1249

Query: 421  WAPLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQ 480
            W+P+  + + RLE PF E+EI +AI  +   K+PGPDG T   F++ W ++K DLV+VF 
Sbjct: 1250 WSPISGESAVRLESPFTEEEICKAIFQMDRDKAPGPDGFTIAVFQDCWEVIKEDLVKVFT 1309

Query: 481  EFFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTT 540
            EF R+GIIN+ TN ++I L+PKK  + +ISDFRPISL+TSLYKI+AKVLA R++++L  T
Sbjct: 1310 EFHRSGIINQSTNASFIVLLPKKSMSRRISDFRPISLITSLYKIIAKVLAGRIREVLHET 1369

Query: 541  ISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKL 600
            I   Q AFVQGR ILD +L+A+E+V+E R   E+GV+FK+DFEKAYD VSW+FLD ++++
Sbjct: 1370 IHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFLDHVMEM 1429

Query: 601  KGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSA 660
            KGFG  WRKW+RGCL++ +F++++NG  +G V ASRGLRQGDPLSPFLFTIV D LSR  
Sbjct: 1430 KGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRML 1489

Query: 661  HYCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLN 720
                E+ VL+G  VGR++T VS LQ+ADDTI FS+  E  M+    +L++    SGL +N
Sbjct: 1490 LKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFSSSREEDMMTLKNVLLVFGHISGLKVN 1549

Query: 721  LAKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKL 780
            L K++I GIN     ++R A    CK    PI  LG PLGGN +T  FWDP++++   +L
Sbjct: 1550 LDKSNIYGINLEQNHLSRLAEMLDCKASGWPILYLGLPLGGNPKTSGFWDPVIERISRRL 1609

Query: 781  EGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPAC 840
            +GW+K  LS GGR+TL QS L  +P Y+ SL K P  V  K+E++ RDF+W+G       
Sbjct: 1610 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEGKRD 1669

Query: 841  NLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGW 900
            +LV W+    P S GG+G G +  RN ALL KWLWR+  E   LW +VI SIYG  S GW
Sbjct: 1670 HLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNGW 1729

Query: 901  RTMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYE 960
                I     R  W  I   Y  F KFT+F V  G RIRFW D W   QPL V +P +  
Sbjct: 1730 DVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPRLLR 1789

Query: 961  ISHMKLASVAECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWK 1020
            +   K A ++         +WN   RR L D E+     L+   D + + + + D+ SW 
Sbjct: 1790 VVTDKNAPISSILGSTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKRSWS 1849

Query: 1021 LEGS--GLFSTKSLFRAAVG 1037
            L  S    F  K + R  VG
Sbjct: 1850 LSSSERDCFMAKRVHRVNVG 1869

BLAST of Lag0002125 vs. NCBI nr
Match: RVW99790.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 976.1 bits (2522), Expect = 2.6e-280
Identity = 476/1080 (44.07%), Postives = 675/1080 (62.50%), Query Frame = 0

Query: 10   GLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLSLDAIGSAGG 69
            GLGS+ KR +VK+FLS E PD+V++QETK +  DRR+V SVWS R+  W +L A G++GG
Sbjct: 367  GLGSKKKRRVVKNFLSSEKPDVVMIQETKKEECDRRLVGSVWSVRNKDWAALPASGASGG 426

Query: 70   ILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSFLQELADVAG 129
            IL++W    +   + VLG++S+S +    G    W++ VYGP +S+ RK F  EL+D+AG
Sbjct: 427  ILIIWDSKKLRREEVVLGSFSVSIKFAMDGCESLWLSSVYGPNNSALRKDFWVELSDIAG 486

Query: 130  LCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHGRFTWSRVGE 189
            L H  WCV GDFN++R   E+L  +R +  M+ F+ FI   +LID P+    +TWS + E
Sbjct: 487  LSHPRWCVGGDFNVIRRSSEKLGGSRLSPCMKDFDEFIRDCELIDSPLRSASYTWSNMQE 546

Query: 190  RAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMPFRFENMWLD 249
                 RLDRFL +++W   F +     L R TSDH+P+ L     +WGP PF+FENMWL 
Sbjct: 547  NPVCKRLDRFLYSNEWEQVFPQSLQGVLPRWTSDHWPIVLETNPFKWGPTPFKFENMWLQ 606

Query: 250  HPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDV 309
            H  F+++  +WW EF   GW G +FM KL+ +K +LKEWNK  FG L++KKK IL  +  
Sbjct: 607  HSSFKENFGRWWSEFQGNGWEGHKFMRKLQFVKAKLKEWNKTSFGELSKKKKDILAVLAN 666

Query: 310  LDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDENSAFFHRWVG 369
             D  E++G +    + +R   K  L E+ + ++    QK ++KW++EGD NS FFH+   
Sbjct: 667  FDSLEQEGGLSHELLVQRAFSKGELEELILREEIHWRQKARVKWVKEGDCNSNFFHKVAN 726

Query: 370  SMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPNWAPLDAQIS 429
              +++ FI  LE+E G  L+    I++EI+ +F  LY       + ++G +W+P+D + +
Sbjct: 727  GRRNRKFIKELENESGLMLNNPESIKEEILKYFEKLYVSPSGESWRVEGLDWSPIDGESA 786

Query: 430  ARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFFRNGIIN 489
            +RLE PF E+EI++AI  +   K+PGPD  T   F++ W+++K DLV VF EF R+GIIN
Sbjct: 787  SRLESPFTEEEIYKAIFQMDRDKAPGPDDFTIAVFQDCWDVIKEDLVRVFTEFHRSGIIN 846

Query: 490  KKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTISDCQAAFV 549
            + TN ++I LIPKK  + +ISDFRPISL+TSLY+I+AKVLA RL+ +L  TI   Q AFV
Sbjct: 847  QSTNASFIVLIPKKSMSRRISDFRPISLITSLYEIIAKVLAGRLRGVLHETIHSTQGAFV 906

Query: 550  QGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLKGFGTTWRK 609
            QGR ILD +L+A+E+V+E R   E+GV+FK+DFEKAYD VSW+FLD +L++KGF   WRK
Sbjct: 907  QGRQILDAVLIANEIVDEKRRTGEEGVVFKIDFEKAYDHVSWDFLDHVLEMKGFSLRWRK 966

Query: 610  WIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVL 669
            W+RGCL++ ++++++NG  +G V ASRGLRQGDPLSPFLFTIV D LSR      E+ VL
Sbjct: 967  WMRGCLSSVSYAVLVNGNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVL 1026

Query: 670  KGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLNLAKTSIIGI 729
            +G  VGR++T VS LQ+ADDTI FS+  E  ++    +L++    SGL +NL K++I GI
Sbjct: 1027 EGFRVGRNRTRVSHLQFADDTIFFSSTREEDLMTLKSVLLVFGHISGLKVNLDKSNIYGI 1086

Query: 730  NTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKLEGWKKLLLS 789
            N     ++R A    CK    PI  LG PLGGN +   FWDP++++   +L+ W+K  LS
Sbjct: 1087 NLEQNHLSRLAVMLDCKASGWPILYLGLPLGGNPKASGFWDPVIERISRRLDVWQKAYLS 1146

Query: 790  KGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWEWTA 849
             GGR+TL QS L  +P Y+ SL K P  V  K+E++ R+F+W+G       +LV W+   
Sbjct: 1147 FGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQREFLWSGVGEGKRDHLVNWDVVC 1206

Query: 850  LPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGWRTMVITHIK 909
             P S GG+G G +  RN ALL KWLWR+  E   LW +VI SIYG  S GW         
Sbjct: 1207 KPKSRGGLGFGKISMRNVALLGKWLWRYRREGSALWHQVILSIYGSHSNGWDVNNNVRWS 1266

Query: 910  GRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYEISHMKLASV 969
             R  W  I   +  F KFT+F V  G RIRFW D W   Q L   +P +  +   K A +
Sbjct: 1267 HRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQTLGTQYPRLLSVVTDKNAPI 1326

Query: 970  AECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWKLEGSGLFST 1029
            +     ++  +WN   RR L D E+    +L+  LD + +   + D+ SW +  SGLF+ 
Sbjct: 1327 SSILGYSHPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPSVPDKRSWSISPSGLFTV 1386

Query: 1030 KSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQRKLKNWALSP 1088
            KS F A    S    +     +W  + P KVK F+W VA++ LNT+D +Q +  + ALSP
Sbjct: 1387 KSFFLALSQYSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDMLQLRRPHKALSP 1446

BLAST of Lag0002125 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 3.1e-58
Identity = 237/953 (24.87%), Postives = 393/953 (41.24%), Query Frame = 0

Query: 1   MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSR----HV 60
           + + + N  G  +  +   V  FL +    +  LQET             W  R    H+
Sbjct: 3   LSISTLNTNGCRNPFRMFQVLSFLRQGGYSVSFLQETHTTPELEASWNLEWKGRVFFNHL 62

Query: 61  GWLSLDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGW-----VTGVYGP 120
            W S        G++ ++ ++       VL A S+      H +++       +  VY P
Sbjct: 63  TWTSC-------GVVTLFSDS---FQPEVLSATSVIPGRLLHLRVRESGRTYNLMNVYAP 122

Query: 121 CSSSDRKSFLQELADVAGLCHG--VWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISS 180
            +  +R  F + L+             + GDFN      +R    +   S       I+ 
Sbjct: 123 TTGPERARFFESLSAYMETIDSDEALIIGGDFNYTLDARDRNVPKKRDSSESVLRELIAH 182

Query: 181 FDLIDIPMNHG----RFTWSRVGE-RAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDH 240
           F L+D+          FT+ RV +   + SR+DR  I+    +  +   + RL  P SDH
Sbjct: 183 FSLVDVWREQNPETVAFTYVRVRDGHVSQSRIDRIYISSHLMSRAQSSTI-RL-APFSDH 242

Query: 241 --FPLALSVGAMRWGPMPFRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFR--------- 300
               L +S+         + F N  L+   F KSV   W      GW  F+         
Sbjct: 243 NCVSLRMSIAPSLPKAAYWHFNNSLLEDEGFAKSVRDTW-----RGWRAFQDEFATLNQW 302

Query: 301 -FMSKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDVLDQ---FEEDGSIQPHHIAERLK 360
             + K+  LK   +E+ K V G  N + + +  ++  L+Q     ED ++Q     E L+
Sbjct: 303 WDVGKVH-LKLLCQEYTKSVSGQRNAEIEALNGEVLDLEQRLSGSEDQALQ----CEYLE 362

Query: 361 LKASLLEITVSDQRRLLQKCKIKWLREGDENSAFFHRWVGSMKSKSFIAALESEEGEFLS 420
            K +L  +     R    + +++ L + D  S FF+       ++  I  L +E+G  L 
Sbjct: 363 RKEALRNMEQRQARGAFVRSRMQLLCDMDRGSRFFYALEKKKGNRKQITCLFAEDGTPLE 422

Query: 421 AEPDIEKEIIGFFTNLYSKD----DSSRFVLDGPNWAPLDAQISARLEMPFKEDEIFRAI 480
               I      F+ NL+S D    D+   + DG     +  +   RLE P   DE+ +A+
Sbjct: 423 DPEAIRDRARSFYQNLFSPDPISPDACEELWDG--LPVVSERRKERLETPITLDELSQAL 482

Query: 481 KSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFFRNGIINKKTNETYICLIPKKRG 540
           + +   KSPG DG+T EFF+ FW+ L PD   V  E F+ G +        + L+PKK  
Sbjct: 483 RLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGD 542

Query: 541 ASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTISDCQAAFVQGRLILDPILVASEVV 600
              I ++RP+SL+++ YKIVAK ++ RLK +L   I   Q+  V GR I D + +  +++
Sbjct: 543 LRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLL 602

Query: 601 EEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLKGFGTTWRKWIRGCLTNSNFSIMIN 660
              R          LD EKA+D+V  ++L   L+   FG  +  +++    ++   + IN
Sbjct: 603 HFARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKIN 662

Query: 661 GKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVLKGMLVGRDQTEVSMLQ 720
                 +   RG+RQG PLS  L+     +L+     CL +K L G+++      V +  
Sbjct: 663 WSLTAPLAFGRGVRQGCPLSGQLY-----SLAIEPFLCLLRKRLTGLVLKEPDMRVVLSA 722

Query: 721 YADDTIIFS----------------AYEESVMLNWWEILMLVLKGSGLSLNLAKTSIIGI 780
           YADD I+ +                A   S  +NW        K SGL     K   +  
Sbjct: 723 YADDVILVAQDLVDLERAQECQEVYAAASSARINW-------SKSSGLLEGSLKVDFL-- 782

Query: 781 NTPSEDMTRWANKFGCKVETLPIKNLG-------YPLGGNHRTRTFWDPIVDKYEAKLEG 840
             P+     W +K         IK LG       YP+  N       +  V     K +G
Sbjct: 783 -PPAFRDISWESKI--------IKYLGVYLSAEEYPVSQNF---IELEECVLTRLGKWKG 842

Query: 841 WKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTM-VIKKLEKLTRDFIWNGGQFKPACN 894
           + K+L  +G  + + Q V +   I+Y  +  +PT   I K+++   DF+W G  +     
Sbjct: 843 FAKVLSMRGRALVINQLVASQ--IWYRLICLSPTQEFIAKIQRRLLDFLWIGKHW----- 897

BLAST of Lag0002125 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 5.9e-57
Identity = 223/931 (23.95%), Postives = 402/931 (43.18%), Query Frame = 0

Query: 3   LISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLSL- 62
           +++ NV GL S  KR  +  ++  ++P +  +QET L   D   +K        GW  + 
Sbjct: 10  ILTLNVNGLNSPIKRHRLASWIKSQDPSVCCIQETHLTCRDTHRLKI------KGWRKIY 69

Query: 63  --DAIGSAGGILLMWKENC----VEVHDSVLGAYSISAECTFHGQIQG---WVTGVYGPC 122
             +      G+ ++  +       ++     G Y +       G IQ     +  +Y P 
Sbjct: 70  QANGKQKKAGVAILVSDKTDFKPTKIKRDKEGHYIM-----VKGSIQQEELTILNIYAPN 129

Query: 123 SSSDRKSFLQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDL 182
           + + R    Q L+D+         + GDFN    + +R    +  K  ++ N  +   DL
Sbjct: 130 TGAPR-FIKQVLSDLQRDLDSHTLIMGDFNTPLSILDRSTRQKVNKDTQELNSALHQTDL 189

Query: 183 IDIP------------MNHGRFTWSR----VGERAAASRLDRF-LITHQWSNTFK---EL 242
           IDI              +    T+S+    VG +A  S+  R  +IT+  S+      EL
Sbjct: 190 IDIYRTLHPKSTEYTFFSAPHHTYSKIDHIVGSKALLSKCKRTEIITNYLSDHSAIKLEL 249

Query: 243 RLDRLQRPTSDHFPLALSVGAMRW--GPMPFRFENMWLDHPDFRKSVEKWWDEFTPTGWA 302
           R+  L +  S  + L   +    W    M    +  +  + +   + +  WD F      
Sbjct: 250 RIKNLTQSRSTTWKLNNLLLNDYWVHNEMKAEIKMFFETNENKDTTYQNLWDAFKAVCRG 309

Query: 303 GFRFMSKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDVLDQFEEDGSIQPHHIAERLKL 362
            F  ++  K  +E+              K  T+  Q+  L++ E+  S +     E  K+
Sbjct: 310 KFIALNAYKRKQER-------------SKIDTLTSQLKELEKQEQTHS-KASRRQEITKI 369

Query: 363 KASLLEITVSDQRRLLQKCKIKWLREGDENSAFFHRWVGSMKSKSFIAALESEEGEFLSA 422
           +A L EI      + + + +  +    ++      R +   + K+ I  +++++G+  + 
Sbjct: 370 RAELKEIETQKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDTIKNDKGDITTD 429

Query: 423 EPDIEKEIIGFFTNLYSKD----DSSRFVLDGPNWAPLDAQISARLEMPFKEDEIFRAIK 482
             +I+  I  ++ +LY+      +     LD      L+ +    L  P    EI   I 
Sbjct: 430 PTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEIVAIIN 489

Query: 483 SLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFFRNGIINKKTNETYICLIPKK-RG 542
           SL   KSPGPDG T EF++ +   L P L+++FQ   + GI+     E  I LIPK  R 
Sbjct: 490 SLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRD 549

Query: 543 ASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTISDCQAAFVQGRLILDPILVASEVV 602
            +K  +FRPISL+    KI+ K+LA R++  +   I   Q  F+ G      I  +  V+
Sbjct: 550 TTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVI 609

Query: 603 EEY-RCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLKGFGTTWRKWIRGCLTNSNFSIMI 662
           +   R K++  V+  +D EKA+DK+   F+   L   G    + K IR        +I++
Sbjct: 610 QHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIIL 669

Query: 663 NGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVLKGMLVGRDQTEVSML 722
           NG+         G RQG PLSP LF IV + L+R+     ++K +KG+ +G+++ ++S+ 
Sbjct: 670 NGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIR---QEKEIKGIQLGKEEVKLSL- 729

Query: 723 QYADDTIIFSAYEESVMLNWWEILMLV---LKGSGLSLNLAKTSIIGINTPSEDMTRWAN 782
            +ADD I+   Y E+ +++   +L L+    K SG  +N+ K+     N   +  ++   
Sbjct: 730 -FADDMIV---YLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYNNNRQTESQIMG 789

Query: 783 KFGCKVETLPIKNLGYPLGGNHRT--RTFWDPIVDKYEAKLEGWKKLLLSKGGRVTLAQS 842
           +    + +  IK LG  L  + +   +  + P++ + +     WK +  S  GR+ + + 
Sbjct: 790 ELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKEDTNKWKNIPCSWVGRINIVKM 849

Query: 843 VLNSLPIYYFSL--LKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWEWTALPTSHGGI 888
            +    IY F+   +K P     +LEK T  FIWN  + + A +++  +  A     GGI
Sbjct: 850 AILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIWNQKRARIAKSILSQKNKA-----GGI 901

BLAST of Lag0002125 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 3.2e-55
Identity = 231/937 (24.65%), Postives = 405/937 (43.22%), Query Frame = 0

Query: 1   MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDR-RIVKSVWSSRHVGWL 60
           + + S NV GL    KR  + D++ K  PD+  +QE+ L   D+ R+    WSS      
Sbjct: 7   LSIFSINVNGLNCPLKRHRLADWIQKLKPDICCIQESHLTLKDKYRLKVKGWSS----IF 66

Query: 61  SLDAIGSAGGILLMWKE----NCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSS 120
             +      GI +++ +       ++     G +      T + +I   +  +Y P  + 
Sbjct: 67  QANGKQKKAGIAILFADAIGFKPTKIRKDKDGHFIFVKGNTQYDEIS--IINIYAP--NH 126

Query: 121 DRKSFLQE-LADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLID 180
           +   F++E L D++ L      V GDFN    V +R +  + +K +   N  I   DL D
Sbjct: 127 NAPQFIRETLTDMSNLISSTSIVVGDFNTPLAVLDRSSKKKLSKEILDLNSTIQHLDLTD 186

Query: 181 I----PMNHGRFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALS 240
           I      N   +T+         S++D  ++ H+ SN  K  +++ +    SDH  + + 
Sbjct: 187 IYRTFHPNKTEYTFFS-SAHGTYSKIDH-ILGHK-SNLSKFKKIEIIPCIFSDHHGIKVE 246

Query: 241 VGAMR--------WGPMPFRFENMWLDHPDFRKSVEKW----------WDEFTPTGWAGF 300
           +   R        W       ++ W+   + +K + K+          +     T  A  
Sbjct: 247 LNNNRNLHTHTKTWKLNNLMLKDTWV-IDEIKKEITKFLEQNNNQDTNYQNLWDTAKAVL 306

Query: 301 RFMSKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDVLDQFEEDGSIQPHHIAERLKLKA 360
           R   K   L+  LK+  +E   NL    K +        + EE  + +P    E  K++A
Sbjct: 307 R--GKFIALQAFLKKTEREEVNNLMGHLKQL--------EKEEHSNPKPSRRKEITKIRA 366

Query: 361 SLLEITVSDQRRLLQ---KCKIKWLREGDENSAFFHRWVGSMKSKSFIAALESEEGEFLS 420
            L EI   + +R++Q   K K  +  + ++            + KS I+++ +   E  +
Sbjct: 367 ELNEI---ENKRIIQQINKSKSWFFEKINKIDKPLANLTRKKRVKSLISSIRNGNDEITT 426

Query: 421 AEPDIEKEIIGFFTNLYS-KDDSSRFV---LDGPNWAPLDAQISARLEMPFKEDEIFRAI 480
              +I+K +  ++  LYS K ++ + +   L+  +   L  +    L  P    EI   I
Sbjct: 427 DPSEIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIASTI 486

Query: 481 KSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFFRNGIINKKTNETYICLIPKK-R 540
           ++L   KSPGPDG T EF++ F   L P L+ +FQ   + GI+     E  I LIPK  +
Sbjct: 487 QNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGK 546

Query: 541 GASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTISDCQAAFVQGRLILDPILVASEV 600
             ++  ++RPISL+    KI+ K+L  R++  +   I   Q  F+ G      I  +  V
Sbjct: 547 DPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINV 606

Query: 601 VEEY-RCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLKGFGTTWRKWIRGCLTNSNFSIM 660
           ++   + KN+  ++  +D EKA+D +   F+   LK  G   T+ K I    +    +I+
Sbjct: 607 IQHINKLKNKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANII 666

Query: 661 INGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVLKGMLVGRDQTEVSM 720
           +NG          G RQG PLSP LF IV + L+ +     E+K +KG+ +G ++ ++S+
Sbjct: 667 LNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIR---EEKAIKGIHIGSEEIKLSL 726

Query: 721 LQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLNLAKT--SIIGINTPSEDMTRWAN 780
             +ADD I++            E++      SG  +N  K+   I   N  +E   + + 
Sbjct: 727 --FADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIYTNNNQAEKTVKDSI 786

Query: 781 KFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEA-------KLEGWKKLLLSKGGRV 840
            F     T+  K + Y   G + T+   D   + YE         +  WK +  S  GR+
Sbjct: 787 PF-----TVVPKKMKYL--GVYLTKDVKDLYKENYETLRKEIAEDVNKWKNIPCSWLGRI 846

Query: 841 TLAQSVLNSLPIYYFSL--LKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWEWTALPT 889
            + +  +    IY F+   +KAP    K LEK+   FIWN  + + A  L+  +  A   
Sbjct: 847 NIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIWNQKKPQIAKTLLSNKNKA--- 901

BLAST of Lag0002125 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 4.7e-54
Identity = 221/932 (23.71%), Postives = 398/932 (42.70%), Query Frame = 0

Query: 3   LISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLSL- 62
           LIS N+ GL S  KR  + D+L K++P    LQET L+  DR  +      R  GW ++ 
Sbjct: 17  LISLNINGLNSPIKRHRLTDWLHKQDPTFCCLQETHLREKDRHYL------RVKGWKTIF 76

Query: 63  --DAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQI---QGWVTGVYGPCSSSD 122
             + +    G+ ++  +  ++    V+            G+I   +  +  +Y P  ++ 
Sbjct: 77  QANGLKKQAGVAILISDK-IDFQPKVIKKDKEGHFILIKGKILQEELSILNIYAP--NAR 136

Query: 123 RKSFLQE-LADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDI 182
             +F+++ L  +         + GDFN      +R    +  +   K    +   DL DI
Sbjct: 137 AATFIRDTLVKLKAYIAPHTIIVGDFNTPLSSKDRSWKQKLNRDTVKLTEVMKQMDLTDI 196

Query: 183 -----PMNHGRFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALS 242
                P   G   +S        S++D  +      N +K + +  +    SDH  L L 
Sbjct: 197 YRTFYPKTKGYTFFS--APHGTFSKIDHIIGHKTGLNRYKNIEI--VPCILSDHHGLRLI 256

Query: 243 VGAMRWGPMP---FRFENMWLDHPDFRKSVEKWWDEFT----------PTGWAGFR--FM 302
                    P   ++  N  L+    ++ ++K   +F           P  W   +    
Sbjct: 257 FNNNINNGKPTFTWKLNNTLLNDTLVKEGIKKEIKDFLEFNENEATTYPNLWDTMKAFLR 316

Query: 303 SKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLL 362
            KL  L    K+       +L    K +        + +E  S +     E +KL+  + 
Sbjct: 317 GKLIALSASKKKRETAHTSSLTTHLKAL--------EKKEANSPKRSRRQEIIKLRGEIN 376

Query: 363 EITVSDQRRLLQKCKIKWLREGDENSAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIE 422
           ++      + + + +  +  + ++      R     + K  I  + +E+G+  +   +I+
Sbjct: 377 QVETRRTIQRINQTRSWFFEKINKIDKPLARLTKGHRDKILINKIRNEKGDITTDPEEIQ 436

Query: 423 KEIIGFFTNLYSK-----DDSSRFVLDGPNWAPLDAQISARLEMPFKEDEIFRAIKSLGP 482
             I  F+  LYS      D+  +F LD      L+      L  P    EI   I SL  
Sbjct: 437 NTIRSFYKRLYSTKLENLDEMDKF-LDRYQVPKLNQDQVDHLNSPISPKEIEAVINSLPT 496

Query: 483 MKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFFRNGIINKKTNETYICLIPK-KRGASKI 542
            KSPGPDG + EF++ F   L P L ++F +    G +     E  I LIPK ++  +KI
Sbjct: 497 KKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEVEGTLPNSFYEATITLIPKPQKDPTKI 556

Query: 543 SDFRPISLVTSLYKIVAKVLAERLKDILPTTISDCQAAFVQGRLILDPILVASEVVEEY- 602
            +FRPISL+    KI+ K+LA R+++ +   I   Q  F+ G      I  +  V+    
Sbjct: 557 ENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKSINVIHYIN 616

Query: 603 RCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLKGFGTTWRKWIRGCLTNSNFSIMINGKP 662
           + K++  ++  LD EKA+DK+   F+  +L+  G    +   I+   +    +I +NG+ 
Sbjct: 617 KLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEK 676

Query: 663 RGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVLKGMLVGRDQTEVSMLQYAD 722
              +    G RQG PLSP+LF IV + L+R+     ++K +KG+ +G+++ ++S+L  AD
Sbjct: 677 LEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIR---QQKEIKGIQIGKEEVKISLL--AD 736

Query: 723 DTIIFSAYEESVMLNWWEILMLV-----LKGSGLSLNLAKTSIIGINTPSEDMTRWANKF 782
           D I++ +  ++   +  E+L L+     + G  ++ N +   +   N  +E   R    F
Sbjct: 737 DMIVYISDPKN---STRELLNLINSFGEVVGYKINSNKSMAFLYTKNKQAEKEIRETTPF 796

Query: 783 GCKVETLPIKNLGYPLGGNHRTRTFWD----PIVDKYEAKLEGWKKLLLSKGGRVTLAQS 842
              + T  IK LG  L      +  +D     +  + +  L  WK L  S  GR+ + + 
Sbjct: 797 --SIVTNNIKYLGVTL--TKEVKDLYDKNFKSLKKEIKEDLRRWKDLPCSWIGRINIVKM 856

Query: 843 VLNSLPIYYFSL--LKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWEWTALPTSHGGI 889
            +    IY F+   +K PT    +LE     F+WN  + + A +L+K + T+     GGI
Sbjct: 857 AILPKAIYRFNAIPIKIPTQFFNELEGAICKFVWNNKKPRIAKSLLKDKRTS-----GGI 909

BLAST of Lag0002125 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-30
Identity = 87/316 (27.53%), Postives = 153/316 (48.42%), Query Frame = 0

Query: 772  IVDKYEAKLEGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIW 831
            I+++  +++ GW++  LS  GR+TL ++VL+S+P++  S +  P  ++ +L++L+R F+W
Sbjct: 16   ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 832  NGGQFKPACNLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGS 891
                 K   +LVKW     P   GG+GV A  S N AL++K  WR   EK  LW  V+  
Sbjct: 76   GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 892  IYGVDSLGWRTMVITHIKGRRLWPNIQRNY-DCFEKFTKFQVSCGRRIRFWGDAWCDVQP 951
             Y V  +     +I        W +I     D       +    G++IRFW D W   +P
Sbjct: 136  KYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKP 195

Query: 952  LQVTFPEIYEISHMKLASVAECWDENNQTWNLGLRRGLFDRELSSWVALIGKLDNIQMGN 1011
            L        E+ + +  +  +     +  W  G  RG    ++  +     +L+   +  
Sbjct: 196  L-------LELDNGERPTDCDTVVAKD-LWIPG--RGWDFAKIDPYTTNNTRLELRAVVL 255

Query: 1012 EM-----DRISWKLEGSGLFSTKSLFR-AAVGKSTKINL-SLAGKIWKHKSPKKVKIFLW 1071
            ++     DR+SWK    G FS +S +    V +  + N+ S    +WK + P++VK FLW
Sbjct: 256  DLVTGARDRLSWKFSQDGQFSVRSAYEMLTVDEVPRPNMASFFNCLWKVRVPERVKTFLW 315

Query: 1072 SVAYRSLNTDDKVQRK 1080
             V  +++ T+++  R+
Sbjct: 316  LVGNQAVMTEEERHRR 321

BLAST of Lag0002125 vs. ExPASy TrEMBL
Match: A0A438GDE7 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=Pol_4 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 7.2e-292
Identity = 496/1127 (44.01%), Postives = 701/1127 (62.20%), Query Frame = 0

Query: 1    MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLS 60
            MK+ISWN RGLGS+ KR +VKDFL  E PD+V+ QETK +  DRR V SVW++R+  W +
Sbjct: 812  MKIISWNTRGLGSKKKRRVVKDFLRSEKPDVVMFQETKKEECDRRFVGSVWTARNKDWAA 871

Query: 61   LDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSF 120
            L A G++GGIL++W    +   + +LG++S+S + T +G    W++ VYGP +S+ RK  
Sbjct: 872  LPACGASGGILIIWDTKKLSREEVMLGSFSVSIKFTLNGCESLWLSAVYGPNNSALRKDL 931

Query: 121  LQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHG 180
              EL+D+AGL    WCV GDFN++R   E+L  +R T SM+ F+ FIS  +LID+P+   
Sbjct: 932  WVELSDIAGLASPRWCVGGDFNVIRRSSEKLGGSRLTPSMKDFDDFISDCELIDLPLRSA 991

Query: 181  RFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMP 240
             FTWS +       RLDRFL +++W  TF +     L R TSDH+P+ L     +WGP P
Sbjct: 992  SFTWSNMQVNPVCKRLDRFLYSNEWEQTFPQSIQGVLPRWTSDHWPIVLETNPFKWGPTP 1051

Query: 241  FRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKK 300
            FRFENMWL HP F+++  +WW EF   GW G +FM KL+ +K +LK WNK  FG L+++K
Sbjct: 1052 FRFENMWLQHPSFKENFGRWWREFQGNGWEGHKFMRKLQFVKAKLKVWNKASFGELSKRK 1111

Query: 301  KTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDEN 360
            + IL  +   D  E++G +    +A+R   K  L E+ + ++    QK ++KW++EGD N
Sbjct: 1112 EDILSALVNFDSLEQEGGLSHELLAQRAIKKGELEELILREEIHWRQKARVKWVKEGDCN 1171

Query: 361  SAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPN 420
            S FFH+     +++ FI  LE+E G+ ++    I++EI+ +F  LY+      + ++G +
Sbjct: 1172 SKFFHKVANGRRNRKFIKELENENGQMMNNSESIKEEILRYFEKLYTSPSGESWRVEGLD 1231

Query: 421  WAPLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQ 480
            W+P+  + + RLE PF E+EI +AI  +   K+PGPDG T   F++ W ++K DLV+VF 
Sbjct: 1232 WSPISGESAVRLESPFTEEEICKAIFQMDRDKAPGPDGFTIAVFQDCWEVIKEDLVKVFT 1291

Query: 481  EFFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTT 540
            EF R+GIIN+ TN ++I L+PKK  + +ISDFRPISL+TSLYKI+AKVLA R++++L  T
Sbjct: 1292 EFHRSGIINQSTNASFIVLLPKKSMSRRISDFRPISLITSLYKIIAKVLAGRIREVLHET 1351

Query: 541  ISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKL 600
            I   Q AFVQGR ILD +L+A+E+V+E R   E+GV+FK+DFEKAYD VSW+FLD ++++
Sbjct: 1352 IHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFLDHVMEM 1411

Query: 601  KGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSA 660
            KGFG  WRKW+RGCL++ +F++++NG  +G V ASRGLRQGDPLSPFLFTIV D LSR  
Sbjct: 1412 KGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRML 1471

Query: 661  HYCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLN 720
                E+ VL+G  VGR++T VS LQ+ADDTI FS+  E  M+    +L++    SGL +N
Sbjct: 1472 LKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFSSSREEDMMTLKNVLLVFGHISGLKVN 1531

Query: 721  LAKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKL 780
            L K++I GIN     ++R A    CK    PI  LG PLGGN +T  FWDP++++   +L
Sbjct: 1532 LDKSNIYGINLEQNHLSRLAEMLDCKASGWPILYLGLPLGGNPKTSGFWDPVIERISRRL 1591

Query: 781  EGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPAC 840
            +GW+K  LS GGR+TL QS L  +P Y+ SL K P  V  K+E++ RDF+W+G       
Sbjct: 1592 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEGKRD 1651

Query: 841  NLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGW 900
            +LV W+    P S GG+G G +  RN ALL KWLWR+  E   LW +VI SIYG  S GW
Sbjct: 1652 HLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNGW 1711

Query: 901  RTMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYE 960
                I     R  W  I   Y  F KFT+F V  G RIRFW D W   QPL V +P +  
Sbjct: 1712 DVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPRLLR 1771

Query: 961  ISHMKLASVAECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWK 1020
            +   K A ++         +WN   RR L D E+     L+   D + + + + D+ SW 
Sbjct: 1772 VVTDKNAPISSILGSTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKRSWS 1831

Query: 1021 LEGSGLFSTKSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQR 1080
            L  SGLF+ KS F A    S    +     +W  + P KVK F+W VA++ +NT+D +Q 
Sbjct: 1832 LSSSGLFTVKSFFLALSQYSVSPPIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDLLQL 1891

Query: 1081 KLKNWALSPRDVDYVLKR----VRILITSSFIVSMLARLGTSLQVCW 1122
            +    ALSP      +K       + +  S  + +  RL  S ++ W
Sbjct: 1892 RRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDW 1938

BLAST of Lag0002125 vs. ExPASy TrEMBL
Match: A0A438FWU5 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_70 PE=4 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 7.4e-289
Identity = 492/1090 (45.14%), Postives = 682/1090 (62.57%), Query Frame = 0

Query: 1    MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLS 60
            MK++SWN RGLGS+ KR +V+ FLS +NPD+V+LQETK +  DRR V SVW  + V W +
Sbjct: 736  MKILSWNTRGLGSKKKRRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKGKRVEWAA 795

Query: 61   LDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSF 120
            L A G++GGI+++W  + +E  + VLG++S++ +     +   W+T VYGP +   RK F
Sbjct: 796  LPACGASGGIVILWDSSKLECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDF 855

Query: 121  LQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHG 180
              EL D+ GL    WCV GDFN++R + E+L  TR T +MR F+ FI    LID P+ + 
Sbjct: 856  WLELQDLYGLTFPRWCVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLIDPPLRNA 915

Query: 181  RFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMP 240
             FTWS +       RLDRFL + +W   F +   + L R TSDH P+ L    ++WGP P
Sbjct: 916  AFTWSNMQADPICKRLDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTP 975

Query: 241  FRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKK 300
            FRFENMWL HP+F++    WW E T  GW G +FM KLK +K +LKEWN   FG+L E+K
Sbjct: 976  FRFENMWLLHPEFKEKFRVWWLECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERK 1035

Query: 301  KTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDEN 360
            K IL  +  +D  E++G++    + ER   +  L ++ + ++ +  QK ++KW++EGD N
Sbjct: 1036 KLILTDLSRIDLIEQEGNLNSDLVLERTLKRRELEDVLLKEEVQWRQKSRVKWIKEGDCN 1095

Query: 361  SAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPN 420
            S FFHR     +S+ FI +L SE GE L+   DI +EI+ FF NLYSK     + ++G +
Sbjct: 1096 SKFFHRVATGRRSRKFIKSLISERGETLNNIEDISEEIVNFFGNLYSKPVGESWRVEGID 1155

Query: 421  WAPLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQ 480
            W P+  +    L+ PF E+E+ RA+  L   K+PGPDG T   ++  W+++K DL+ VF 
Sbjct: 1156 WVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAPGPDGFTIAVYQECWDVIKEDLMRVFL 1215

Query: 481  EFFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTT 540
            EF  NG+IN+ TN T+I L+PKK  + KISD+RPISLVTSLYKI+AKVL+ RL+ +L  T
Sbjct: 1216 EFHTNGVINQSTNATFIALVPKKSQSVKISDYRPISLVTSLYKIIAKVLSGRLRKVLHET 1275

Query: 541  ISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKL 600
            ISD Q AFV+GR ILD +L+A+EVV+E R   E+G++FK+DFEKAYD V W FLD +L+ 
Sbjct: 1276 ISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQR 1335

Query: 601  KGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSA 660
            KGF   WR WIRGCL++S+F+I++NG  +G V ASRGLRQGDPLSPFLFT+V D LSR  
Sbjct: 1336 KGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKASRGLRQGDPLSPFLFTLVADVLSRML 1395

Query: 661  HYCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLN 720
                E  + +G  VGRD+T VS+LQ+ADDTI FS      + N   IL++  + SGL +N
Sbjct: 1396 FRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFSKASMEHLQNLKIILLVFGQVSGLKIN 1455

Query: 721  LAKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKL 780
            L K++I GINT  E ++  A+ F C+V   P+  LG PLGGN +T  FWDP+V++   +L
Sbjct: 1456 LEKSTISGINTRQELLSSLASVFDCRVSEWPLSYLGLPLGGNPKTIGFWDPVVERISRRL 1515

Query: 781  EGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPAC 840
            +GWKK  LS GGR+TL QS L+ +P Y+ SL K P  +  K+EK+ R+F+W+G       
Sbjct: 1516 DGWKKAYLSLGGRITLIQSCLSHIPSYFLSLFKIPASIASKIEKMQRNFLWSGAGEGKKD 1575

Query: 841  NLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGW 900
            +LV+WE  + P   GG+G G +  RN ALL KWLWRF  E+ GLW +VIGSIYG    GW
Sbjct: 1576 HLVRWEVVSRPKELGGLGFGKISLRNIALLGKWLWRFPRERSGLWYKVIGSIYGTHPNGW 1635

Query: 901  RTMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYE 960
               ++     R  W  I + +  F  F +  V  G RIRFW D W   Q L   F ++Y 
Sbjct: 1636 DANMVVRWSHRCPWKAIAQVFQEFSPFVRLVVGNGERIRFWEDLWWGNQSLCSQFADLYR 1695

Query: 961  ISHMKLASVAECWDEN-NQTWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWK 1020
            +  +K  +V+     +    WNL  RR L D E+     L+  L +++    + D  +W 
Sbjct: 1696 VISVKNLTVSNVLGNSFPLAWNLNFRRNLTDSEIDLLQRLMSSLSSVRFFPSLADSRAWS 1755

Query: 1021 LEGSGLFSTKSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQR 1080
            L  SGLF+ KS F A    S  I    A  +W  K P KVK   W VA+  +NT+DK+Q 
Sbjct: 1756 LSSSGLFTVKSFFLALSKVSNPILFLPAKFLWSSKVPSKVKALAWIVAHGKVNTNDKLQL 1815

Query: 1081 KLKNWALSPR 1089
            +    +L P+
Sbjct: 1816 RRPYKSLCPQ 1825

BLAST of Lag0002125 vs. ExPASy TrEMBL
Match: A5BPI6 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_008538 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 4.0e-282
Identity = 483/1088 (44.39%), Postives = 671/1088 (61.67%), Query Frame = 0

Query: 2    KLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLSL 61
            K+ISWN RGLGSR KR +VKDFL  E PD+V++QETK    DRR V SVW++R+  W  L
Sbjct: 113  KIISWNTRGLGSRKKRRVVKDFLRSEKPDIVMIQETKKAECDRRFVGSVWTARNKEWAVL 172

Query: 62   DAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSFL 121
             A G++GGIL++W    +   + VLG++S+S +    G  Q W + VYGP S++ RK F 
Sbjct: 173  PACGASGGILVIWDSKKLHSEEVVLGSFSVSVKFAVDGSEQFWXSAVYGPNSTALRKDFW 232

Query: 122  QELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHGR 181
             EL+D+ GL    WCV GDFN++R   E+L   R T SM+  + FI   +LID P+    
Sbjct: 233  VELSDIFGLSSPCWCVGGDFNVIRRCSEKLGGGRLTPSMKDLDDFIRENELIDPPLRSAS 292

Query: 182  FTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMPF 241
            FTWS + E     RLDRFL +++W   F +   + L R TSDH+P+ L     +WGP PF
Sbjct: 293  FTWSNMQEHPVCKRLDRFLYSNEWEQLFPQSLQEVLPRWTSDHWPIVLETNPFKWGPTPF 352

Query: 242  RFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKKK 301
            RFENMWL HP F++   +WW EF   GW G +FM KL+ LK +LKEWNK  FG+L E+KK
Sbjct: 353  RFENMWLHHPSFKECFGRWWREFQGDGWEGHKFMRKLQFLKAKLKEWNKNAFGDLIERKK 412

Query: 302  TILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDENS 361
             IL  I   D  E++G + P  + +R   K  L E+ + ++    QK ++KW++EGD NS
Sbjct: 413  CILLDIANFDSMEQEGGLSPELLIQRAVRKGELEELILREEIHWRQKARVKWVKEGDCNS 472

Query: 362  AFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPNW 421
              FH+     +++ FI  LE+E G  L     I++EI+ +F  LY+      + ++G +W
Sbjct: 473  KXFHKVANGRRNRKFIKVLENERGLVLDNSDSIKEEILRYFEKLYASPSGESWRVEGLDW 532

Query: 422  APLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQE 481
            +P+  + ++RLE PF E+EI++AI  +    +PGPDG T   F++ W+++K DLV VF E
Sbjct: 533  SPISRESASRLESPFTEEEIYKAIFQMDRDXAPGPDGFTIAVFQDCWDVIKEDLVRVFDE 592

Query: 482  FFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTI 541
            F R+GIIN+ TN ++I L+PKK  A KIS++RPISL+TSLYKI+AKVLA RL+ IL  TI
Sbjct: 593  FHRSGIINQSTNASFIVLLPKKSMAKKISNYRPISLITSLYKIIAKVLAGRLRGILHETI 652

Query: 542  SDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLK 601
               Q AFVQGR ILD +L+A+E+V+E +   E+GV+FK+DFEKAYD VSW+FLD +++ K
Sbjct: 653  HSTQGAFVQGRQILDAVLIANEIVDEKKRSGEEGVVFKIDFEKAYDHVSWDFLDHVMEKK 712

Query: 602  GFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAH 661
            GF    RKWIR CL++ +F+I++NG  +G V   RGLRQGDPLSPFLFTIV D  S    
Sbjct: 713  GFNPXXRKWIRXCLSSVSFAILVNGNAKGWVKXXRGLRQGDPLSPFLFTIVADVXSXMLL 772

Query: 662  YCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLNL 721
               E+ V +G  VGR++T VS LQ+ADDTI FS+  E  +L    +L +    SGL +NL
Sbjct: 773  RAEERNVFEGFRVGRNRTRVSHLQFADDTIFFSSTREEDLLTLKSVLXVFGHISGLKVNL 832

Query: 722  AKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKLE 781
             K++I GIN   + + R A    CK    PI  LG PLGGN ++ +FWDP++++  ++L+
Sbjct: 833  DKSNIYGINLGQDHLHRLAELLDCKASGWPILYLGLPLGGNPKSGSFWDPVIERISSRLD 892

Query: 782  GWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPACN 841
            GW+K  LS GGR+TL QS L  +P Y+ SL K P  V  ++E+L RDF+W+G       +
Sbjct: 893  GWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAGRIERLQRDFLWSGVGEGKRDH 952

Query: 842  LVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGWR 901
            LV W+        GG+G+G +  RN+ALL KWLWR+  E   LW +VI SIYG  S GW 
Sbjct: 953  LVSWBVVCKSKMKGGLGLGRISLRNSALLGKWLWRYPREGSALWHQVILSIYGSHSNGWD 1012

Query: 902  TMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYEI 961
                     R  W  I + +  F KFT+F V  G RIRFW D W   Q L V FP +  +
Sbjct: 1013 ANTXVRWSHRCPWKAIAQVFQDFSKFTRFIVGDGDRIRFWEDLWWGDQSLGVRFPRLLRV 1072

Query: 962  SHMKLASVAECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWKL 1021
               K   ++         +WN   RR L D E+    +L+  LD+I +   + D+ SW L
Sbjct: 1073 VMDKNILISSILGSTRPFSWNFNFRRNLSDSEIEKVESLMQSLDHIHLSPSVPDKRSWSL 1132

Query: 1022 EGSGLFSTKSLFRAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQRK 1081
              SGLF+ KS F A    S   ++     +W  + P K+K F+W VA++ +NT+D +Q +
Sbjct: 1133 SSSGLFTVKSFFLALSQISGLPSVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTNDMLQLR 1192

Query: 1082 LKNWALSP 1088
                ALSP
Sbjct: 1193 RPYKALSP 1200

BLAST of Lag0002125 vs. ExPASy TrEMBL
Match: M5VS59 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016504mg PE=4 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 5.2e-282
Identity = 493/1135 (43.44%), Postives = 696/1135 (61.32%), Query Frame = 0

Query: 16   KRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLSLDAIGSAGGILLMWK 75
            KR+LVK+ L +  PD+VIL ETK + +DR++V  VW SR   W+   ++G +GGI ++W 
Sbjct: 2    KRLLVKEQLRRLKPDIVILLETKKETVDRQLVAGVWGSRFKEWVFSPSLGRSGGIAVLWN 61

Query: 76   ENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSFLQELADVAGLCHGVW 135
               V V DS++G +S+S     +     W++G+YGPC   +R SF +ELAD+ G C   W
Sbjct: 62   SQSVSVIDSMVGEFSVSIRIVENIGTDWWLSGIYGPCRQRERNSFWEELADLYGFCGDKW 121

Query: 136  CVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHGRFTWSRVGERAAASR 195
            C+ GDFN+VR+  E+ N  R TKSMR FN FI   +L D  + +  FTWS + E A   R
Sbjct: 122  CLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPNLLNASFTWSNLRENAVCRR 181

Query: 196  LDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMPFRFENMWLDHPDFRK 255
            LDRFL++  W + F   R   L R TSDH P+ L    ++WGP PFRFENMWL+HPDF +
Sbjct: 182  LDRFLVSGSWEDHFPHYRHKALPRITSDHCPIELDTSRVKWGPSPFRFENMWLNHPDFMR 241

Query: 256  SVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDVLDQFEE 315
             ++ WW E    GW G++FM++LK LK +LK W+KE FG++    +    ++ VLDQ E 
Sbjct: 242  KIKLWWGEDQIPGWEGYKFMTRLKMLKSKLKVWSKEEFGDVERDLREAEARLLVLDQREG 301

Query: 316  DGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDENSAFFHRWVGSMKSKS 375
               +     +ER  L   + ++   ++ +  Q+ K+KW REGD N+ FFHR     + ++
Sbjct: 302  TEGLDHLLRSERDNLLLKIGDLAQKEEVKWRQRGKVKWAREGDGNTKFFHRVANGARKRN 361

Query: 376  FIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPNWAPLDAQISARLEMP 435
            +I  LE E+   +  + +IE+E+I FF  LYS + +  + ++G NW P+    +  LE P
Sbjct: 362  YIEKLEVEDLGVIEVDANIEREVIRFFKGLYSSNKNVGWGVEGLNWCPISQVEADWLERP 421

Query: 436  FKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFFRNGIINKKTNET 495
            F  +E+ +A+   G  KSPGPDG +  FF++ W ++K DL++V Q+FF++GI+N  TNET
Sbjct: 422  FDLEEVQKAVFECGKDKSPGPDGFSMSFFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNET 481

Query: 496  YICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTTISDCQAAFVQGRLIL 555
            +ICLIPKK  + K++D RPISLVTSLYK+++KVLA RL+++L  TIS  Q AFVQ R IL
Sbjct: 482  FICLIPKKANSVKVTDNRPISLVTSLYKVISKVLASRLREVLGNTISQSQGAFVQKRQIL 541

Query: 556  DPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKLKGFGTTWRKWIRGCL 615
            D +LVA+EVVEE R +  KG++FK+DFEKAYD V W F+D +L  KGFG  WR WI GCL
Sbjct: 542  DAVLVANEVVEEVRKQKRKGLVFKIDFEKAYDHVEWNFVDDVLARKGFGVKWRGWIIGCL 601

Query: 616  TNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVLKGMLVG 675
             + NFSIMINGKPRGK  ASRGLRQGDPLSPFLFT+V D LSR      +  ++ G++ G
Sbjct: 602  ESVNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSG 661

Query: 676  RDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLNLAKTSIIGINTPSED 735
             DQ EVS LQ+ADDTI     +E   LN  ++L L    SG+ +N AK+ I+GIN  +E 
Sbjct: 662  HDQVEVSHLQFADDTIFLLDGKEEYWLNLLQLLKLFCDVSGMKINKAKSCILGINFSTEA 721

Query: 736  MTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKLEGWKKLLLSKGGRVT 795
            +   A  +GC+V   P+  LG PLGGN R   FW+P++DK E +L+ WK+  LSKGGR+T
Sbjct: 722  LNNMAGSWGCEVGCWPMVYLGLPLGGNPRALNFWNPVMDKVEKRLQKWKRACLSKGGRLT 781

Query: 796  LAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWEWTALPTSHG 855
            L Q+VL+S+P YY SL K P  V  K+E+L R+F+W G +    C+LV+WE        G
Sbjct: 782  LIQAVLSSIPSYYMSLFKMPIGVAAKVEQLMRNFLWEGLEEGKNCHLVRWERVTKSKEEG 841

Query: 856  GIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGWRTMVITHIKGRRLWP 915
            G+G+G+L  RN AL  KWLWRF  E   LW R+I S YG+DS GW T  I  +  R  W 
Sbjct: 842  GLGIGSLRERNEALRAKWLWRFPLEPNSLWHRIIKSKYGIDSNGWDTKQIDKVSCRNPWR 901

Query: 916  NIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYEISHMKLASVAECWDE 975
             I + Y+ F +  +F V  G +IRFW D W     L+  FP +  +S  K  S+A C+  
Sbjct: 902  EISKGYNSFLQCCRFSVGNGEKIRFWEDLWLKEGILKDLFPRLSSLSRRKNQSIA-CFAN 961

Query: 976  NN---QTWNLGLRRGLFDRELSSWVALIGKLDNIQM-GNEMDRISWKLEGSGLFSTKSLF 1035
            N+     W+   RR L + E++  V L+  L N+++ G+  DR SW++E  G FS KS F
Sbjct: 962  NHVMPLNWDFDFRRNLSEAEIAEVVILLDILGNVRLYGSRPDRRSWEVEEQGSFSCKS-F 1021

Query: 1036 RAAVGKSTKINLSLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKVQRKLKNWALSP---- 1095
            R+ +  +T+        IWK K+P K++ F+W  A   +NT D +QR+     LSP    
Sbjct: 1022 RSFLLSTTRDVFPPFSSIWKAKTPPKIQFFVWLAANGRINTCDCIQRRQPKMRLSPSWCV 1081

Query: 1096 ------RDVDYVLKRVRILITSSFIVSMLARLG--------TSLQVCWGYLSAYQ 1129
                   ++D++   +    +      ML  LG        + +++C G+L +YQ
Sbjct: 1082 LCKENAENIDHLF--IHCSYSLRLWWRMLGALGVEWNSLNFSPIKLCLGFLKSYQ 1132

BLAST of Lag0002125 vs. ExPASy TrEMBL
Match: A5CAA2 (Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_030956 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 7.5e-281
Identity = 470/1040 (45.19%), Postives = 658/1040 (63.27%), Query Frame = 0

Query: 1    MKLISWNVRGLGSRSKRMLVKDFLSKENPDLVILQETKLQHIDRRIVKSVWSSRHVGWLS 60
            MK+ISWN RGLGS+ KR +VKDFL  E PD+V+ QETK +  DRR V SVW++R+  W +
Sbjct: 830  MKIISWNTRGLGSKKKRRVVKDFLRSEKPDVVMFQETKKEECDRRFVGSVWTARNKDWAA 889

Query: 61   LDAIGSAGGILLMWKENCVEVHDSVLGAYSISAECTFHGQIQGWVTGVYGPCSSSDRKSF 120
            L A G++GGIL++W    +   + +LG++S+S + T +G    W++ VYGP +S+ RK  
Sbjct: 890  LPACGASGGILIIWDTKKLSREEVMLGSFSVSIKFTLNGCESLWLSAVYGPNNSALRKDL 949

Query: 121  LQELADVAGLCHGVWCVSGDFNMVRWVDERLNATRPTKSMRKFNRFISSFDLIDIPMNHG 180
              EL+D+AGL    WCV GDFN++R   E+L  +R T SM+ F+ FIS  +LID+P+   
Sbjct: 950  WVELSDIAGLASPRWCVGGDFNVIRRSSEKLGGSRLTPSMKDFDDFISDCELIDLPLRSA 1009

Query: 181  RFTWSRVGERAAASRLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAMRWGPMP 240
             FTWS +       RLDRFL +++W  TF +     L R TSDH+P+ L     +WGP P
Sbjct: 1010 SFTWSNMQVNPVCKRLDRFLYSNEWEQTFPQSIQGVLPRWTSDHWPIVLETNPFKWGPTP 1069

Query: 241  FRFENMWLDHPDFRKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKK 300
            FRFENMWL HP F+++  +WW EF   GW G +FM KL+ +K +LK WNK  FG L+++K
Sbjct: 1070 FRFENMWLQHPSFKENFGRWWREFQGNGWEGHKFMRKLQFVKAKLKVWNKASFGELSKRK 1129

Query: 301  KTILDQIDVLDQFEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDEN 360
            + IL  +   D  E++G +    +A+R   K  L E+ + ++    QK ++KW++EGD N
Sbjct: 1130 EDILSALVNFDSLEQEGGLSHELLAQRAIKKGELEELILREEIHWRQKARVKWVKEGDCN 1189

Query: 361  SAFFHRWVGSMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKDDSSRFVLDGPN 420
            S FFH+     +++ FI  LE+E G+ ++    I++EI+ +F  LY+      + ++G +
Sbjct: 1190 SKFFHKVANGRRNRKFIKELENENGQMMNNSESIKEEILRYFEKLYTSPSGESWRVEGLD 1249

Query: 421  WAPLDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQ 480
            W+P+  + + RLE PF E+EI +AI  +   K+PGPDG T   F++ W ++K DLV+VF 
Sbjct: 1250 WSPISGESAVRLESPFTEEEICKAIFQMDRDKAPGPDGFTIAVFQDCWEVIKEDLVKVFT 1309

Query: 481  EFFRNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIVAKVLAERLKDILPTT 540
            EF R+GIIN+ TN ++I L+PKK  + +ISDFRPISL+TSLYKI+AKVLA R++++L  T
Sbjct: 1310 EFHRSGIINQSTNASFIVLLPKKSMSRRISDFRPISLITSLYKIIAKVLAGRIREVLHET 1369

Query: 541  ISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGVLFKLDFEKAYDKVSWEFLDAILKL 600
            I   Q AFVQGR ILD +L+A+E+V+E R   E+GV+FK+DFEKAYD VSW+FLD ++++
Sbjct: 1370 IHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFLDHVMEM 1429

Query: 601  KGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSA 660
            KGFG  WRKW+RGCL++ +F++++NG  +G V ASRGLRQGDPLSPFLFTIV D LSR  
Sbjct: 1430 KGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRML 1489

Query: 661  HYCLEKKVLKGMLVGRDQTEVSMLQYADDTIIFSAYEESVMLNWWEILMLVLKGSGLSLN 720
                E+ VL+G  VGR++T VS LQ+ADDTI FS+  E  M+    +L++    SGL +N
Sbjct: 1490 LKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFSSSREEDMMTLKNVLLVFGHISGLKVN 1549

Query: 721  LAKTSIIGINTPSEDMTRWANKFGCKVETLPIKNLGYPLGGNHRTRTFWDPIVDKYEAKL 780
            L K++I GIN     ++R A    CK    PI  LG PLGGN +T  FWDP++++   +L
Sbjct: 1550 LDKSNIYGINLEQNHLSRLAEMLDCKASGWPILYLGLPLGGNPKTSGFWDPVIERISRRL 1609

Query: 781  EGWKKLLLSKGGRVTLAQSVLNSLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPAC 840
            +GW+K  LS GGR+TL QS L  +P Y+ SL K P  V  K+E++ RDF+W+G       
Sbjct: 1610 DGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEGKRD 1669

Query: 841  NLVKWEWTALPTSHGGIGVGALGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGW 900
            +LV W+    P S GG+G G +  RN ALL KWLWR+  E   LW +VI SIYG  S GW
Sbjct: 1670 HLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHSNGW 1729

Query: 901  RTMVITHIKGRRLWPNIQRNYDCFEKFTKFQVSCGRRIRFWGDAWCDVQPLQVTFPEIYE 960
                I     R  W  I   Y  F KFT+F V  G RIRFW D W   QPL V +P +  
Sbjct: 1730 DVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPRLLR 1789

Query: 961  ISHMKLASVAECWDENNQ-TWNLGLRRGLFDRELSSWVALIGKLDNIQMGNEM-DRISWK 1020
            +   K A ++         +WN   RR L D E+     L+   D + + + + D+ SW 
Sbjct: 1790 VVTDKNAPISSILGSTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKRSWS 1849

Query: 1021 LEGS--GLFSTKSLFRAAVG 1037
            L  S    F  K + R  VG
Sbjct: 1850 LSSSERDCFMAKRVHRVNVG 1869

BLAST of Lag0002125 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 1.9e-42
Identity = 113/402 (28.11%), Postives = 190/402 (47.26%), Query Frame = 0

Query: 139 GDFNMVRWVDER---LNATRPTKSMRKFNRFISSFDLIDIPMNHGRFTWS-RVGERAAAS 198
           GDF+ +    +    L  + P + + +F   +   DL+DIP     +TWS    +     
Sbjct: 225 GDFDQIAATSDHYSVLQTSIPMRGLEEFQNCLRDSDLVDIPSRGVHYTWSNHQDDNPIIR 284

Query: 199 RLDRFLITHQWSNTFKELRLDRLQRPTSDHFPLALSVGAM-RWGPMPFRFENMWLDHPDF 258
           +LDR +    W ++F            SDH P  + +  + +     FR+ +    HP F
Sbjct: 285 KLDRAIANGDWFSSFPSAIAVFELSGVSDHSPCIIILENLPKRSKKCFRYFSFLSTHPTF 344

Query: 259 RKSVEKWWDEFTPTGWAGFRFMSKLKGLKEQLKEWNKEVFGNLNEKKKTILDQIDVLDQ- 318
             S+   W+E  P G   F     LK  K+  K  N++ FGN+  K K  LD ++ +   
Sbjct: 345 LVSLTVAWEEQIPVGSHMFSLGEHLKAAKKCCKLLNRQGFGNIQHKTKEALDSLESIQSQ 404

Query: 319 ---FEEDGSIQPHHIAERLKLKASLLEITVSDQRRLLQKCKIKWLREGDENSAFFHRWVG 378
                 D   +  H+A +   K +     +    R  QK +IKWL++GD N+ FFH+ + 
Sbjct: 405 LLTNPSDSLFRVEHVARK---KWNFFAAALESFYR--QKSRIKWLQDGDANTRFFHKVIL 464

Query: 379 SMKSKSFIAALESEEGEFLSAEPDIEKEIIGFFTNLYSKD------DSSRFVLDGPNWAP 438
           + ++K+ I  L  ++   +     +++ I+ ++T+L   D      DS + + D   +  
Sbjct: 465 ANQAKNLIKFLRMDDDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRC 524

Query: 439 LDAQISARLEMPFKEDEIFRAIKSLGPMKSPGPDGMTGEFFKNFWNILKPDLVEVFQEFF 498
            D  +++RL     + EI  A+ ++   K+PGPD  T EFF   W ++K   +   +EFF
Sbjct: 525 NDT-LASRLSALPSDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFF 584

Query: 499 RNGIINKKTNETYICLIPKKRGASKISDFRPISLVTSLYKIV 526
           R G + K+ N T I LIPK  G  ++S FRP+S  T +YKI+
Sbjct: 585 RTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

BLAST of Lag0002125 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 95.9 bits (237), Expect = 2.2e-19
Identity = 80/333 (24.02%), Postives = 133/333 (39.94%), Query Frame = 0

Query: 750  LPIKNLGYPLGGNHRTRTFWDPIVDKYEAKLEGWKKLLLSKGGRVTLAQSVLNSLPIYYF 809
            LP++ LG PL     T + + P+V+K   ++  W    LS  GR+ L  SV++SL  ++ 
Sbjct: 23   LPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWM 82

Query: 810  SLLKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWEWTALPTSHGGIGVGALGSRNNAL 869
            S  + P+  IK+++ +   F+W+G +       V W     P   GG+G+ +L   N   
Sbjct: 83   SAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEAN--- 142

Query: 870  LTKWLWRFAHEKEGLWRRVIGSIYGVDSLG-WRTMVITHIKGRRLWPNIQRNYDCFEKFT 929
                       K   W     SI G  +LG W            +W  I ++      F 
Sbjct: 143  -----------KGSFW-----SISGNTTLGSW------------MWKKILKHRALASGFV 202

Query: 930  KFQVSCGRRIRFWGDAWCDV-QPLQVT-FPEIYEISHMKLASVAECWDENNQTWNLGLRR 989
            K  +  G    FW D W  + + + VT      ++     ASVAE         N   RR
Sbjct: 203  KHDIHNGSNTSFWFDNWSKIGRLIDVTGHRGCIDMGITLHASVAEA------VVNHRPRR 262

Query: 990  GLFDRELSSWVALIGKLDNIQMGNEMDRISWKLEG---SGLFSTKSLFRAAVGKSTKINL 1049
               D  L     +I ++ +  + +  D + WK  G      F+TK  + A   +  K+ +
Sbjct: 263  HRHD-TLLRIEDVIAEVRHQGLTSGEDTVRWKGNGDIFKPCFNTKETWAAT--REPKLKV 315

Query: 1050 SLAGKIWKHKSPKKVKIFLWSVAYRSLNTDDKV 1077
            +    +W   +  K  +  W      L T D++
Sbjct: 323  NWYKGVWFSHATPKYSVLAWIAIKNRLTTGDRM 315

BLAST of Lag0002125 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 64.7 bits (156), Expect = 5.5e-10
Identity = 58/203 (28.57%), Postives = 92/203 (45.32%), Query Frame = 0

Query: 529 LAERLKDILPTTISDCQAAFVQGRLILDPILVASEVVEEYRCKNEKGV----LFKLDFEK 588
           + ERLK ++   I   QA+F+ GR+  D I+   E V   R K  KGV    L KLD EK
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRK--KGVKGWMLLKLDLEK 60

Query: 589 AYDKVSWEFLDAILKLKGFGTTWRKWIRGCLTNSNFSIMINGKPRGKVYASRGLRQGDPL 648
           AYD++ W++L+  L   GF   W   I      S F         G+  AS+  R  D  
Sbjct: 61  AYDRIRWDYLEDTLISAGFPEVWLPEI----ARSTFGARRVAPEVGRADASKRPRVSDHR 120

Query: 649 SPFLFTIVGDALSRSAHYCLEKKVLKGMLVGRDQTEVSM-LQYADDTIIFSAYEESV-ML 708
             F +  +    + ++  C E  +L+   +GR     SM L   ++ +   A  +S+  +
Sbjct: 121 WGFRYDDMAAPFTSNSVACAE--LLRE--IGRGSGIPSMPLDLKEEKVFAGAARKSIEAI 180

Query: 709 NWWEILMLVLKGSGLSLNLAKTS 726
             W+ L  + +    SL++  T+
Sbjct: 181 AEWDRLTYLYERCVRSLSVEATN 193

BLAST of Lag0002125 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 63.5 bits (153), Expect = 1.2e-09
Identity = 31/68 (45.59%), Postives = 42/68 (61.76%), Query Frame = 0

Query: 623 MINGKPRGKVYASRGLRQGDPLSPFLFTIVGDALSRSAHYCLEKKVLKGMLVGRDQTEVS 682
           +ING P+G V  SRGLRQGDPLSP+LF +  + LS       E+  L G+ V  +   ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 683 MLQYADDT 691
            L +ADDT
Sbjct: 73  HLLFADDT 80

BLAST of Lag0002125 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 55.8 bits (133), Expect = 2.6e-07
Identity = 40/151 (26.49%), Postives = 61/151 (40.40%), Query Frame = 0

Query: 803 SLPIYYFSLLKAPTMVIKKLEKLTRDFIWNGGQFKPACNLVKWE-WTALPTSHGGIGVGA 862
           +LP+Y  S  +   ++ KKL     +F W+  + K   + V W+         GG+G   
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 863 LGSRNNALLTKWLWRFAHEKEGLWRRVIGSIYGVDSLGWRTMVITHIKGRRLWPNIQRNY 922
           LG  N ALL K  +R  H+   L  R++ S Y   S      V T  +    W +I    
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGT--RPSYAWRSIIHGR 121

Query: 923 DCFEKFTKFQVSCGRRIRFWGDAW-CDVQPL 952
           +   +     +  G   + W D W  D  PL
Sbjct: 122 ELLSRGLLRTIGDGIHTKVWLDRWIMDETPL 150