Lag0000486 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0000486 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 147.1 bits (370), Expect = 2.8e-31
Identity = 110/386 (28.50%), Postives = 147/386 (38.08%), Query Frame = 0

Query: 9    GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
            GF++ WI  I+ CIST  FSI +NG   G   PSRG+RQGDPLSPYLFLLC+EG S+L++
Sbjct: 759  GFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGIRQGDPLSPYLFLLCAEGLSALIN 818

Query: 69   TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCINF 128
               NS  L+G+    ++  ++HLLF DDS +FL+S   E    + L+  Y RASGQCINF
Sbjct: 819  HENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLESECLALRRLLDSYGRASGQCINF 878

Query: 129  S----------------------------------------------------------- 188
            S                                                           
Sbjct: 879  SKSALLFSPNVHPERQQYLQCILNVKLVSHFGNYLGLPSHFTRRRGESRKLHWMKWGRMC 938

Query: 189  ------------------------------------------------------------ 233
                                                                        
Sbjct: 939  YPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSS 998

BLAST of Lag0000486 vs. NCBI nr
Match: XP_030970961.1 (uncharacterized protein LOC115991405 [Quercus lobata])

HSP 1 Score: 142.5 bits (358), Expect = 6.8e-30
Identity = 67/119 (56.30%), Postives = 91/119 (76.47%), Query Frame = 0

Query: 9   GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
           GFHD+W  LI+ CI++ S+S+++NG + G+I PSRGLRQGDPLSPYLFLLC++GFSSL+S
Sbjct: 500 GFHDSWTCLIMRCITSVSYSVLINGVAFGNIIPSRGLRQGDPLSPYLFLLCADGFSSLIS 559

Query: 69  TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCIN 128
             V +++LSGLS+ R   K+SHL F DDS +F K+N++E      ++  YE ASGQ IN
Sbjct: 560 KAVRNQMLSGLSICRGGPKISHLFFADDSLLFCKANSHECQQLTEILCLYEAASGQKIN 618

BLAST of Lag0000486 vs. NCBI nr
Match: XP_030958760.1 (uncharacterized protein LOC115980671 [Quercus lobata])

HSP 1 Score: 141.4 bits (355), Expect = 1.5e-29
Identity = 66/119 (55.46%), Postives = 90/119 (75.63%), Query Frame = 0

Query: 9   GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
           GFHD+W  LI+ CI++ S+S+++NG + G+I PSRGLRQGDPLSPYLFLLC++GFSSL+S
Sbjct: 500 GFHDSWTCLIMHCITSVSYSVLINGVAFGNIIPSRGLRQGDPLSPYLFLLCADGFSSLIS 559

Query: 69  TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCIN 128
             V +++LSGLS+ R   K+SHL F DDS +F K+N++E      ++  YE  SGQ IN
Sbjct: 560 KAVRNQMLSGLSICRGGPKISHLFFADDSLLFCKANSHECQQLTEILCLYEATSGQKIN 618

BLAST of Lag0000486 vs. NCBI nr
Match: XP_030478262.1 (uncharacterized protein LOC115695328 [Cannabis sativa])

HSP 1 Score: 137.5 bits (345), Expect = 2.2e-28
Identity = 62/119 (52.10%), Postives = 86/119 (72.27%), Query Frame = 0

Query: 9   GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
           GFHD W+SLI++C+ + SFS ++NGE +GH+ PSRGLRQGDPLSPYLFL+CSEG S LL 
Sbjct: 123 GFHDRWVSLIMNCLHSTSFSFMLNGEEVGHVQPSRGLRQGDPLSPYLFLICSEGLSRLLQ 182

Query: 69  TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCIN 128
           +  +S  L GL L+RH+  +SHLLF DD+ +F ++    +     ++  Y +ASGQ +N
Sbjct: 183 SEESSNNLKGLRLTRHAPSISHLLFADDNLLFCEATNSSARAINKVLDIYHKASGQLLN 241

BLAST of Lag0000486 vs. NCBI nr
Match: XP_023880426.1 (uncharacterized protein LOC111992797 [Quercus suber])

HSP 1 Score: 135.6 bits (340), Expect = 8.3e-28
Identity = 63/119 (52.94%), Postives = 90/119 (75.63%), Query Frame = 0

Query: 9   GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
           GF++NWI L++ CIS+ S+S+++NG + G+I+P+RGLRQGDPLS YLFLLC+EGFS+L++
Sbjct: 27  GFNENWIMLVMKCISSVSYSVIINGTTYGNITPNRGLRQGDPLSLYLFLLCAEGFSALIN 86

Query: 69  TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCIN 128
              ++  L+G+S+ R + KVSHL F DDS +F K+N  E    K ++  YE ASGQ IN
Sbjct: 87  EVASNNQLNGISICRGAPKVSHLFFADDSIIFCKANGNECNKLKEILGLYESASGQKIN 145

BLAST of Lag0000486 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.9e-12
Identity = 35/73 (47.95%), Postives = 48/73 (65.75%), Query Frame = 0

Query: 25 FSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLSTTVNSRLLSGLSLSRH 84
          F    ++NG   G ++PSRGLRQGDPLSPYLF+LC+E  S L         L G+ +S +
Sbjct: 8  FYLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNN 67

Query: 85 SAKVSHLLFIDDS 98
          S +++HLLF DD+
Sbjct: 68 SPRINHLLFADDT 80

BLAST of Lag0000486 vs. ExPASy TrEMBL
Match: A0A803P5H2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.0e-35
Identity = 95/232 (40.95%), Postives = 122/232 (52.59%), Query Frame = 0

Query: 9   GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
           GF   WISLI+ C+ T SFS ++NGE  G + P RGLRQGDPLSPYLFL+CSEG S LL 
Sbjct: 660 GFGIKWISLIMTCLHTNSFSFLINGEVSGSVIPQRGLRQGDPLSPYLFLICSEGLSRLLQ 719

Query: 69  TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCIN- 128
                  L GL++SRHS  ++HLLF DDS +F ++N    G  K  +  Y RASGQ +N 
Sbjct: 720 HEEQIGRLQGLAVSRHSPSITHLLFADDSLLFCQANDRSCGSIKRALDVYHRASGQLLNT 779

Query: 129 --FSFVWGLELLHKGLRKNLGNGKEIRMFKDPRLPLSSTFKVV----------------- 188
                VWG ELL KGL   +G+G  +   +D  +P +  FK +                 
Sbjct: 780 DKSGIVWGRELLSKGLIIKIGDGTGVNCAQDSWIPRNDYFKPLRFTGSCSNLVADYITDT 839

Query: 189 -------------SSNADFIKNFPMS-NFTPDIWVWHSGRFGKYFVRSGYKV 207
                         ++ D I   P+S N T D W WH    G Y V+SGY +
Sbjct: 840 REWDLELLHNDFSPADIDRILTIPLSYNSTRDRWRWHYDSSGDYTVKSGYNL 891

BLAST of Lag0000486 vs. ExPASy TrEMBL
Match: A0A803NML1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 3.8e-34
Identity = 89/191 (46.60%), Postives = 113/191 (59.16%), Query Frame = 0

Query: 9    GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
            GF   WISLI+ CIST SFS  +NG+ +GH++P RGLRQGDPLSPYLFL+CSEG S  L 
Sbjct: 1054 GFDHLWISLIMRCISTSSFSFSLNGDIVGHVTPHRGLRQGDPLSPYLFLICSEGLSRFLQ 1113

Query: 69   TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCINF 128
                S +L GL L+R++  VSHLLF DDS +F +S    +   K  +  Y RASGQ    
Sbjct: 1114 AQEQSGMLKGLCLTRNAPSVSHLLFADDSLLFCQSTEQSAISIKRSLDTYHRASGQ---- 1173

Query: 129  SFVWGLELLHKGLRKNLGNGKEIRMFKDPRLPLSSTFKVVSSNADFIKNFPMSNFTPDIW 188
                  ELL KGLR  +G+G  +   KDP +P  + FK VS       + P+S+F  D  
Sbjct: 1174 ------ELLLKGLRFKIGDGIHVDNRKDPWIPSHNDFKPVSYLGS--DSLPVSHFITDNR 1232

Query: 189  VWHSGRFGKYF 200
            VW+      YF
Sbjct: 1234 VWNVPLLTSYF 1232

BLAST of Lag0000486 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.3e-31
Identity = 110/386 (28.50%), Postives = 147/386 (38.08%), Query Frame = 0

Query: 9    GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
            GF++ WI  I+ CIST  FSI +NG   G   PSRG+RQGDPLSPYLFLLC+EG S+L++
Sbjct: 759  GFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGIRQGDPLSPYLFLLCAEGLSALIN 818

Query: 69   TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCINF 128
               NS  L+G+    ++  ++HLLF DDS +FL+S   E    + L+  Y RASGQCINF
Sbjct: 819  HENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLESECLALRRLLDSYGRASGQCINF 878

Query: 129  S----------------------------------------------------------- 188
            S                                                           
Sbjct: 879  SKSALLFSPNVHPERQQYLQCILNVKLVSHFGNYLGLPSHFTRRRGESRKLHWMKWGRMC 938

Query: 189  ------------------------------------------------------------ 233
                                                                        
Sbjct: 939  YPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSS 998

BLAST of Lag0000486 vs. ExPASy TrEMBL
Match: A0A2N9F9Z6 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11476 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.3e-30
Identity = 86/259 (33.20%), Postives = 130/259 (50.19%), Query Frame = 0

Query: 9   GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
           GFH  W+S++++C+ T S+S+++NG+      PSRGLRQGDP+SPYLFLLC+EG  +LL+
Sbjct: 146 GFHSKWVSMVMECVRTVSYSVLVNGDPKRFFHPSRGLRQGDPISPYLFLLCAEGLQALLA 205

Query: 69  TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCIN- 128
           +  +S  + GLS+S     ++HL F DDS +F ++  +     + ++  YER SGQ IN 
Sbjct: 206 SAASSNQIQGLSISCGGPVLTHLFFADDSVLFCRATLFNCHAIQEILRKYERVSGQQINH 265

Query: 129 ------FSFVWGLEL---LHKGLR-KNLGNGKEIRMFKDPRLPLSSTFKVVS-------- 188
                 FS    LE    +   L    +GNG  I+++ D  LP  S    V+        
Sbjct: 266 DKTILFFSAATSLETQDEIQTALHLLRVGNGVSIQIWSDKWLPFPSAGVPVTPFNTLPED 325

Query: 189 -------------------------SNADFIKNFPMSNFTP-DIWVWHSGRFGKYFVRSG 223
                                      A  IK+  +S   P D+ VW   R G+Y VRS 
Sbjct: 326 ACVSQLIQATTGTWDNAMVDRVFLPGEASLIKSMVLSYRAPEDLLVWCGKRSGRYSVRSA 385

BLAST of Lag0000486 vs. ExPASy TrEMBL
Match: A0A2N9FR55 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17283 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.8e-29
Identity = 89/294 (30.27%), Postives = 142/294 (48.30%), Query Frame = 0

Query: 9    GFHDNWISLIVDCISTFSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLS 68
            GF   W+ LI++C+ST S+S+++NG    +I P RGLRQGDPLSPYLFLL +EG +SLL 
Sbjct: 787  GFESQWVDLIMECLSTVSYSVLLNGTPKSYIVPRRGLRQGDPLSPYLFLLYAEGLTSLLQ 846

Query: 69   TTVNSRLLSGLSLSRHSAKVSHLLFIDDSPVFLKSNAYESGLFKSLVLDYERASGQCINF 128
               + R + G+++S+   ++SH+LF+DDS +F K+   E    K ++  YE A G C+  
Sbjct: 847  KAESQRQVHGIAISQGGPRISHILFVDDSILFCKATMQECRALKDILSLYENAFGWCV-- 906

Query: 129  SFVWGLELLHKGLRKNLGNGKEIRMFKDPRLPLSSTF------KVVSSNADF-------- 188
                             G    IR+++D  +P   TF      K++S NA          
Sbjct: 907  -----------------GASSRIRIWRDRWIPTKMTFKIQSPVKILSENATVDSLIQLPT 966

Query: 189  -------------------IKNFPMSNF-TPDIWVWHSGRFGKYFVRSGYKVFM---LSK 248
                               IK+ P+S + +PD+ +W   + G + V+S Y + M    ++
Sbjct: 967  RQWNVPLIDAVFSTTEVATIKSIPLSRWASPDVLIWSETKNGVHSVKSAYHLLMEVNQAR 1026

Query: 249  VEGAPSSLANGLLKESSPTKLVAPFI-----QDRNGVVIHVDVPCNHHHNITGF 261
              G  S++++G L      K    +      +D    ++HV V C     + GF
Sbjct: 1027 DVGESSNISHGRLLWKDIWKRALNYFSSGVCEDDPKSIVHVLVDCCFAREVWGF 1061

BLAST of Lag0000486 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 73.9 bits (180), Expect = 2.8e-13
Identity = 35/73 (47.95%), Postives = 48/73 (65.75%), Query Frame = 0

Query: 25 FSFSIVMNGESIGHISPSRGLRQGDPLSPYLFLLCSEGFSSLLSTTVNSRLLSGLSLSRH 84
          F    ++NG   G ++PSRGLRQGDPLSPYLF+LC+E  S L         L G+ +S +
Sbjct: 8  FYLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNN 67

Query: 85 SAKVSHLLFIDDS 98
          S +++HLLF DD+
Sbjct: 68 SPRINHLLFADDT 80