Lag0000286 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0000286 vs. NCBI nr
Match: XP_030508852.1 (uncharacterized protein LOC115723496 [Cannabis sativa])

HSP 1 Score: 321.6 bits (823), Expect = 8.8e-84
Identity = 173/426 (40.61%), Postives = 233/426 (54.69%), Query Frame = 0

Query: 1    MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
            + C++SV YS L+NG+    + P RG+ QGDPLSPYLFLI AE LS LL  E+      G
Sbjct: 620  LRCLQSVSYSFLLNGTIQGQVIPSRGIRQGDPLSPYLFLICAEGLSRLLQYEELAGSLEG 679

Query: 61   ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
            + I+   PS+SHLFFADD ++FCRA+++  R+I + L  Y   S Q  N EK +   S+N
Sbjct: 680  LKISRSAPSVSHLFFADDSVLFCRANQQSARAIHRCLITYSRASGQVINPEKCVLSFSEN 739

Query: 121  IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
                +     DLLG+        YLG+PS + + K  LF    +K+ K L  WKE LFS 
Sbjct: 740  TRQHEQIFFKDLLGMPIQPCHEQYLGLPSFSGKNKKQLFGGITDKIWKLLSSWKEHLFSA 799

Query: 181  GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
            GGKE L+KA+VQAIPTY MSCF+LP   C  I    ARFWW S+   + IHW NW+ LCK
Sbjct: 800  GGKEVLLKAVVQAIPTYAMSCFRLPVTLCHQIESMMARFWWGSTATGKTIHWKNWNFLCK 859

Query: 241  SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
            +K  GG+ F +   FNQA+LAKQ+W+IL+ PN LLS +L  RYF +G+YL A +G NPSL
Sbjct: 860  AKVQGGLGFRNFIHFNQALLAKQAWRILEFPNSLLSNLLRHRYFSNGNYLIAGLGSNPSL 919

Query: 301  TWRSILWGRDLFL----------------------AHS---------------------- 358
            TWRS++WG++L L                       H+                      
Sbjct: 920  TWRSLVWGKELLLKGLRWRVGSGERINCKTDSWLPGHTTFKPYFFKGPDPNLLVADLITE 979

BLAST of Lag0000286 vs. NCBI nr
Match: XP_030509188.1 (uncharacterized protein LOC115723863 [Cannabis sativa])

HSP 1 Score: 317.4 bits (812), Expect = 1.7e-82
Identity = 169/385 (43.90%), Postives = 226/385 (58.70%), Query Frame = 0

Query: 1    MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
            + C+ +V YS L+NGS    + P RG+ QGDPLSPYLFLI AE LS LL  E+S     G
Sbjct: 620  LRCLTTVTYSFLLNGSIKGLVTPSRGIRQGDPLSPYLFLICAEGLSRLLQHEESTGALQG 679

Query: 61   ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
            + I+   PS+SHLFFADD ++FCRA+ +  RSI + LQ Y   S Q  N +K +   S N
Sbjct: 680  LKISRNAPSVSHLFFADDSVLFCRANRQSARSIHRCLQTYSQASGQVINPDKCVLSFSDN 739

Query: 121  IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
                +    + LLG+        YLG+PS   R K  LF    +K+ K L  WKE LFS 
Sbjct: 740  TKRHEQDFFTALLGMPIQPCHEQYLGLPSFAGRDKKKLFGGITDKIWKLLSSWKEHLFSA 799

Query: 181  GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
            GGKE L+KA+VQAIPTY MSCF+LP   C  I    A FWW SS   + IHW NW+ LCK
Sbjct: 800  GGKEILLKAVVQAIPTYAMSCFRLPITLCHQIESMMANFWWGSSASGKSIHWKNWNFLCK 859

Query: 241  SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
            +K  GG+ F +   FNQA+LAKQ+W++++ PN LLSK+L  RYF +G +L++ +G NPSL
Sbjct: 860  AKVQGGLGFRNFIHFNQALLAKQAWRLIEFPNSLLSKLLRHRYFSNGRFLSSGLGSNPSL 919

Query: 301  TWRSILWGRDLFLAH-----------SVRA-----------------YEQQNEIIWAENS 358
            TWRS+    +L +A            S+RA                 +   + +IW+ + 
Sbjct: 920  TWRSLGADPNLLVADLITEQRQWDLISLRANFSQPDVDRILSIPLSLFPHDDAMIWSHSF 979

BLAST of Lag0000286 vs. NCBI nr
Match: XP_030478973.1 (uncharacterized protein LOC115696051 [Cannabis sativa])

HSP 1 Score: 316.6 bits (810), Expect = 2.8e-82
Identity = 168/401 (41.90%), Postives = 216/401 (53.87%), Query Frame = 0

Query: 1    MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
            M C+ +   S L+NGS + ++KP RG+ QGDPLSPYLFL+ AE LSSLL   ++     G
Sbjct: 939  MKCITTSNLSFLINGSITGSVKPSRGIRQGDPLSPYLFLLCAEGLSSLLCAHQNSGSLKG 998

Query: 61   ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
            + I+   PSISHL FADD LIFC A  + C S+ +IL +Y   S Q  N  KS  + S N
Sbjct: 999  VAISRRAPSISHLLFADDSLIFCAADRESCNSLSRILNLYSLASGQVINFAKSSILFSPN 1058

Query: 121  IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
               D          ++    +  YLG+P   AR K   F   K+KV   L  W    FS+
Sbjct: 1059 TPQDVRNLFFTTFDLEDRPFVSKYLGLPQCLARSKYQSFAFLKDKVLSVLTNWSGKWFSK 1118

Query: 181  GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
             GKE L+KA++QAIP Y M+CF+LP   C  I  A ARFWW SS  N KIHW +W +LC+
Sbjct: 1119 AGKEILLKAVIQAIPAYVMACFRLPVKLCKGIEAAMARFWWGSSGNNHKIHWKSWKKLCQ 1178

Query: 241  SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
            SK++GG+ F  +  FNQAMLAKQ+WKI K P+ LL ++L  RYF +   L A  GHNPS 
Sbjct: 1179 SKSMGGLGFRSLIHFNQAMLAKQAWKIFKQPDSLLGRVLKARYFPNSSLLEAIPGHNPSY 1238

Query: 301  TWRSILWGRDLFLA----------------------HSVRAYEQQN-------------- 360
             WRSILWGRDL                         +    Y+  +              
Sbjct: 1239 AWRSILWGRDLMALGLIWKIGDGKNILTIGDHWLPNNKYLCYKDDHVPPSPKLSFFISDS 1298

Query: 361  --EIIWAENSKGVFFVKSAYHLAVSVAEANEASCSVNEMFF 364
              E+IW+    G+F VKSAYHLA+S  +    S   N   F
Sbjct: 1299 GYELIWSRAGSGLFTVKSAYHLALSYQDIPSTSSDTNSRKF 1339

BLAST of Lag0000286 vs. NCBI nr
Match: XP_030498122.1 (uncharacterized protein LOC115713779 [Cannabis sativa])

HSP 1 Score: 312.0 bits (798), Expect = 7.0e-81
Identity = 151/313 (48.24%), Postives = 202/313 (64.54%), Query Frame = 0

Query: 1   MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
           + C++SV YS L+NG     +KP RG+ QGDPLSPYLFLI AE LS LL  +++     G
Sbjct: 620 LRCLQSVSYSFLLNGKVLGDLKPSRGIRQGDPLSPYLFLICAEGLSRLLQSKENNGSLYG 679

Query: 61  ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
           + ++   PS+SHLFFADD ++FCRA+ +  RSI+++L +Y   S Q  N +K +   S N
Sbjct: 680 LRVSRTAPSVSHLFFADDSVLFCRANPQSARSIQQVLDIYHRASGQAVNPDKCVLSFSPN 739

Query: 121 IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
                      LL +        YLG+PS + R K  LF+   +K+ K L  WKE LFS 
Sbjct: 740 TRQYHQNYFQQLLNMPIQPCHEEYLGLPSFSGRDKTRLFSGITDKIWKLLSSWKEQLFSA 799

Query: 181 GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
           GGKE L+KA+VQAIPTY MSCF+LP   C  I    A FWW S+     IHW NW+ LCK
Sbjct: 800 GGKEVLLKAVVQAIPTYAMSCFRLPATLCHQIESMMANFWWGSTASGNAIHWKNWNSLCK 859

Query: 241 SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
           +K  GG+ F +   FNQA+LAKQ+W++L++PN LLS+IL  RYF HG  L+A +G+ PSL
Sbjct: 860 AKVQGGLGFRNFIDFNQALLAKQAWRLLESPNSLLSRILHHRYFLHGSLLSAGLGNCPSL 919

Query: 301 TWRSILWGRDLFL 314
           TWRSI+WG++L +
Sbjct: 920 TWRSIVWGKELLV 932

BLAST of Lag0000286 vs. NCBI nr
Match: XP_030483769.1 (uncharacterized protein LOC115700339 [Cannabis sativa])

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-80
Identity = 146/318 (45.91%), Postives = 208/318 (65.41%), Query Frame = 0

Query: 1   MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
           M C+ +  +S  +NG  S ++ P+RGL QGDPLSPYLFLI +E LS LL  E+++    G
Sbjct: 585 MTCLHTNSFSFAINGEVSGSVIPQRGLRQGDPLSPYLFLICSEGLSRLLKYEENIGRLQG 644

Query: 61  ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
           + ++   P+ISHL FADD L+FC+A+++ C +I++ L +Y   S Q  N +KS+   S N
Sbjct: 645 LAVSRHSPTISHLLFADDSLLFCQATDRSCGAIKRALDIYHRASGQELNTDKSVMSFSPN 704

Query: 121 IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
             +        +LG+   +    YLG+P+ + R K+ LF+  KEK+ K +  W + +FS 
Sbjct: 705 TPMFVQNSFQQILGMPICECHETYLGLPAYSERDKSQLFSHIKEKIWKLMHAWNDKIFSI 764

Query: 181 GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
           GGKE L+KA+VQ+IPTY MSCF+L K FC+++    ARFWW S+  N+KIHW  W  LCK
Sbjct: 765 GGKEVLLKAVVQSIPTYAMSCFRLSKKFCNEVEAMMARFWWGSATDNKKIHWKKWKFLCK 824

Query: 241 SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
           SK  GGM F     FNQA+LAKQ+W+I + PN LLS++L GRY+ H D++ A+     SL
Sbjct: 825 SKGDGGMGFRSFVHFNQALLAKQAWRIFQQPNSLLSRVLKGRYYPHSDFMTASANGLCSL 884

Query: 301 TWRSILWGRDLFLAHSVR 319
           TW+ I+WGR+L LA  +R
Sbjct: 885 TWQGIVWGREL-LAKGLR 901

BLAST of Lag0000286 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.5e-30
Identity = 58/120 (48.33%), Postives = 78/120 (65.00%), Query Frame = 0

Query: 193 AIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCKSKA-LGGMDFWD 252
           A+P Y MSCF+L K  C  +  A   FWW S +  RKI W+ W +LCKSK   GG+ F D
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 253 ISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSLTWRSILWGRDL 312
           +  FNQA+LAKQS++I+  P+ LLS++L  RYF H   +  ++G  PS  WRSI+ GR+L
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

BLAST of Lag0000286 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.5e-17
Identity = 50/175 (28.57%), Postives = 86/175 (49.14%), Query Frame = 0

Query: 147 MPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQGGKETLIKAIVQAIPTYTMSCFKLPK 206
           MP    R     F +  E+V   + GW+E   S  G+ TL KA++ ++P ++MS   LP+
Sbjct: 1   MPVLQKRINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQ 60

Query: 207 FFCDDINRACARFWWVSSQGNRKIHWMNWHRLCKSKALGGMDFWDISLFNQAMLAKQSWK 266
              + +++    F W S+   +K H + W ++C  K  GG+        N+A+++K  W+
Sbjct: 61  SILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWR 120

Query: 267 ILKNPNCLLSKILMGRYFKHGDYLNATIGHNP----SLTWRSILWGRDLFLAHSV 318
           +L+  N L + +L  +Y  H   +  +    P    S TWRSI  G    ++H V
Sbjct: 121 LLQEKNSLWTLVLQKKY--HVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGV 173

BLAST of Lag0000286 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 3.2e-12
Identity = 70/261 (26.82%), Postives = 122/261 (46.74%), Query Frame = 0

Query: 10  SILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNGICINNLYPS 69
           +I VNG   E I  + G  QG PLSPYLF I  EVL+  + ++K +    GI I      
Sbjct: 647 NIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKEI---KGIQIGKEEVK 706

Query: 70  ISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSI-FMTSKNIGVDK-IK 129
           IS L  ADD +++    +   R +  ++  +        N  KS+ F+ +KN   +K I+
Sbjct: 707 ISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMAFLYTKNKQAEKEIR 766

Query: 130 GLSDL-LGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQGGKETL 189
             +   +   + K +G  L    ++   KN  F   K+++ + L+ WK+   S  G+  +
Sbjct: 767 ETTPFSIVTNNIKYLGVTLTKEVKDLYDKN--FKSLKKEIKEDLRRWKDLPCSWIGRINI 826

Query: 190 IKAIV--QAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCKSKAL 249
           +K  +  +AI  +     K+P  F +++  A  +F W     N K   +    L   +  
Sbjct: 827 VKMAILPKAIYRFNAIPIKIPTQFFNELEGAICKFVW-----NNKKPRIAKSLLKDKRTS 886

Query: 250 GGMDFWDISLFNQAMLAKQSW 266
           GG+   D+ L+ +A++ K +W
Sbjct: 887 GGITMPDLKLYYRAIVIKTAW 895

BLAST of Lag0000286 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.2e-12
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 0

Query: 12 LVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNGICINNLYPSIS 71
          ++NG+P   + P RGL QGDPLSPYLF++  EVLS L  R +      GI ++N  P I+
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 72 HLFFADD 79
          HL FADD
Sbjct: 73 HLLFADD 79

BLAST of Lag0000286 vs. ExPASy TrEMBL
Match: A0A2N9J4E8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS59377 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 2.8e-83
Identity = 156/365 (42.74%), Postives = 226/365 (61.92%), Query Frame = 0

Query: 1   MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
           M C+ +V+YS+L++G P   I P RGL QGDPLSPYLFL+ AE LS+L+ +  ++    G
Sbjct: 251 MECIRTVQYSVLLDGVPKGFIIPSRGLRQGDPLSPYLFLLCAEGLSALMRKAGTMGRLKG 310

Query: 61  ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
              + + P +SHLFFADD L+F +AS  + +   +ILQ+YEA+S Q  N EK+    S N
Sbjct: 311 TKTSRVGPWVSHLFFADDSLLFGKASVAESQEFMRILQVYEASSGQQLNREKTAIFFSSN 370

Query: 121 IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
              +  + + DL G   T++   YLG+P+   R K S+FN  KE++ + LQGWKE   S+
Sbjct: 371 TVPETRQAIQDLWGSNGTQNFDKYLGLPALIGRSKRSIFNGLKERIVRRLQGWKEKFLSK 430

Query: 181 GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
            G+E LIKA+ Q+IPTYTM+CF+LPK +CD++N   A++WW  S+  RKIHW+ W +LC 
Sbjct: 431 AGREILIKAVAQSIPTYTMNCFRLPKAWCDEVNSLIAKYWWGQSKDERKIHWLKWDKLCI 490

Query: 241 SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
           SK  GG+ F ++ +FN A+LAKQ W++L N   L  ++   +YF    +++A +G  PS 
Sbjct: 491 SKEEGGLGFRNLHMFNTALLAKQCWRLLTNQQSLFFRVFKAKYFPSCSFVDAQLGSAPSF 550

Query: 301 TWRSILWGRDLFLAHSVRAYE--QQNEIIWAENSKGVFFVKSAYHLAVSVAEA-NEASCS 360
            WRS L GRDL L      Y      +++W+E   G+F V+SAY L        N   CS
Sbjct: 551 IWRSFLSGRDLLLKGIRWVYTPGALPKMLWSETKSGLFTVRSAYQLLEKTQRVENSGECS 610

Query: 361 VNEMF 363
            +E +
Sbjct: 611 NSENY 615

BLAST of Lag0000286 vs. ExPASy TrEMBL
Match: A0A2N9GD63 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25220 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 6.2e-83
Identity = 155/365 (42.47%), Postives = 225/365 (61.64%), Query Frame = 0

Query: 1    MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
            M C+ +V+YS+L++G P   I P RGL QGDPLSPYLFL+  E LS+L+ +  ++    G
Sbjct: 841  MECIRTVQYSVLLDGVPKGFIIPSRGLRQGDPLSPYLFLLCVEGLSALMRKAGTMGRLKG 900

Query: 61   ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
               + + P +SHLFFADD L+F +AS  + +   +ILQ+YEA+S Q  N EK+    S N
Sbjct: 901  TKTSRVGPWVSHLFFADDSLLFGKASVAESQEFMRILQLYEASSGQQLNREKTAIFFSSN 960

Query: 121  IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
               +  + + DL G   T++   YLG+P+   R K S+FN  KE++ + LQGWKE   S+
Sbjct: 961  TVPETRQAIQDLWGSNGTQNFDKYLGLPALIGRSKRSIFNGLKERIVRRLQGWKEKFLSK 1020

Query: 181  GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
             G+E LIKA+ Q+IPTYTM+CF+LPK +CD++N   A++WW  S+  RKIHW+ W +LC 
Sbjct: 1021 AGREILIKAVAQSIPTYTMNCFRLPKAWCDEVNSLIAKYWWGQSKDERKIHWLKWDKLCT 1080

Query: 241  SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
            SK  GG+ F ++ +FN A+LAKQ W++L N   L  ++   +YF    +++A +G  PS 
Sbjct: 1081 SKEEGGIGFRNLHMFNTALLAKQCWRLLTNQQSLFFRVFKAKYFPSCSFVDAQLGSAPSF 1140

Query: 301  TWRSILWGRDLFLAHSVRAYE--QQNEIIWAENSKGVFFVKSAYHLAVSVAEA-NEASCS 360
             WRS L GRDL L      Y      +++W+E   G+F V+SAY L        N   CS
Sbjct: 1141 IWRSFLSGRDLLLKGIRWVYTPGALPKMMWSETKSGLFTVRSAYQLLEKTQRVENSGECS 1200

Query: 361  VNEMF 363
             +E +
Sbjct: 1201 NSENY 1205

BLAST of Lag0000286 vs. ExPASy TrEMBL
Match: A0A2N9GRT8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30234 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.2e-81
Identity = 149/350 (42.57%), Postives = 217/350 (62.00%), Query Frame = 0

Query: 1    MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
            M C+ +V+YS+L++G P   I P RG+ QGDPLSPY+FL+ AE LS++L +     H  G
Sbjct: 1410 MECIRTVKYSVLIDGVPKGFITPTRGIRQGDPLSPYVFLLCAEGLSAMLQKASLSGHLKG 1469

Query: 61   ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
            + +    P +SHLFFADD L+F +A+ ++C  + +IL +YE +S Q  N++K+    S N
Sbjct: 1470 LQLCRGGPWVSHLFFADDSLLFGQATIEECSKMLEILNLYERSSGQKINMDKTAIHFSTN 1529

Query: 121  IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
               D    +    G +   +   YLG+P+   R K S+FN  KE++ + LQGWKE   S+
Sbjct: 1530 TAQDNRLAIRAFWGSQGVNNFDKYLGLPAMIGRSKKSIFNGLKERIVQRLQGWKERFLSK 1589

Query: 181  GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
             G+E LIKA+ QAIPTY M+CF+LPK +CD++N   AR+WW   +  RK+HW+ W +LC 
Sbjct: 1590 AGREILIKAVAQAIPTYAMNCFRLPKSWCDEVNGLIARYWWGQQKDERKLHWIKWGKLCT 1649

Query: 241  SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
            +KA GG+ F ++S FN A+LAKQ W+IL  P  L  ++   RYF +  +++A +G NPS 
Sbjct: 1650 AKADGGLGFRNLSSFNSALLAKQCWRILHYPQSLFFRVFKARYFPNCSFIDAQLGSNPSF 1709

Query: 301  TWRSILWGRDLFLAHSVRAYEQQNEI------IWAENSKGVFFVKSAYHL 345
             WRS LWGRD      ++  E Q++        W   S G+F VKSAY +
Sbjct: 1710 LWRSFLWGRDTL----IKGLEWQSQTGQPLRPRWKATSSGIFTVKSAYDM 1755

BLAST of Lag0000286 vs. ExPASy TrEMBL
Match: A0A803NTN0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 3.4e-81
Identity = 167/425 (39.29%), Postives = 223/425 (52.47%), Query Frame = 0

Query: 1   MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
           MNC+ +  +S  +NG     ++P RGL QGDPLSPYLFLI +E  S LL  E+S  +  G
Sbjct: 320 MNCITTSSFSFSLNGEVVGHVQPHRGLRQGDPLSPYLFLICSEGFSRLLQYEESAGNLEG 379

Query: 61  ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
           + +    PS+SHL FADD L+FCRA+++   +I++IL  Y   S Q  N  KS+   S N
Sbjct: 380 LRLTRNAPSVSHLLFADDSLLFCRATDQSATAIKRILDTYHKASGQALNNNKSVMSFSPN 439

Query: 121 IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
                    ++ L +  T+    YLG+PS + R K  LF+  KEKV K L  W E +FS 
Sbjct: 440 TSPAAQNFFANTLHMPITECHERYLGLPSYSGRDKQELFSHIKEKVWKLLHAWNEQIFSA 499

Query: 181 GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
           GGKE L+KA+VQ+IPTY MSCFKL K FC  +    A FWW ++Q   KIHW  W  LCK
Sbjct: 500 GGKEVLLKAVVQSIPTYAMSCFKLTKKFCSQLESMMANFWWGTNQNGTKIHWKRWKALCK 559

Query: 241 SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
           SK  GGM F     FNQA+LAKQ+W+I   PN LLS++L  RYF +  +L+A IGH+PS 
Sbjct: 560 SKHEGGMGFRSFVHFNQALLAKQAWRIFDMPNSLLSRLLKHRYFSNNSFLDAHIGHSPSY 619

Query: 301 TWRSILWGRDL------------------------------------------------- 358
           TW+SI WGRDL                                                 
Sbjct: 620 TWQSICWGRDLLVKGVRFKVGNGTNIACAIDPWIPSHTNFKPVSFQGSSSLSVSTFITDQ 679

BLAST of Lag0000286 vs. ExPASy TrEMBL
Match: A0A803NM27 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 9.9e-81
Identity = 142/311 (45.66%), Postives = 201/311 (64.63%), Query Frame = 0

Query: 1    MNCMESVEYSILVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNG 60
            +NC+++ + S ++NG+ S  +KP+RGL QGDPLSPYLFLI +E LS LL  E+S+    G
Sbjct: 1029 LNCLQTTQLSFIINGAISGNVKPQRGLRQGDPLSPYLFLICSEGLSRLLQYEESIGCLKG 1088

Query: 61   ICINNLYPSISHLFFADDRLIFCRASEKDCRSIRKILQMYEATSEQTTNLEKSIFMTSKN 120
            + ++   PS+SHL FADD L+FC A ++ C +I+++L  Y   S Q  N +KS+   S N
Sbjct: 1089 LAVSRHSPSVSHLLFADDSLLFCEADDRSCGAIKRVLDTYHKASGQQLNTDKSVMSFSPN 1148

Query: 121  IGVDKIKGLSDLLGIKHTKSIGHYLGMPSQNARRKNSLFNQRKEKVGKTLQGWKESLFSQ 180
                  +   ++LG+   +    YLG+P+ + R K  LFNQ KE++ K L  W + +FS 
Sbjct: 1149 TSEASKQSFQNILGMPICECHESYLGLPAYSERDKKQLFNQIKERIWKLLNAWTDKIFSV 1208

Query: 181  GGKETLIKAIVQAIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCK 240
            GGKE L+KA++Q+IPTY MSCFKLP  FC +I    + FWW S+   +KIHW  W  LCK
Sbjct: 1209 GGKEVLLKAVIQSIPTYAMSCFKLPVKFCHEIESLMSNFWWGSTSDKKKIHWKQWKFLCK 1268

Query: 241  SKALGGMDFWDISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSL 300
            SK  GG+ F +   FNQA+LAKQ+W++ +NP  LL ++L GRYF   D+L+A      SL
Sbjct: 1269 SKLEGGLGFRNFIHFNQALLAKQAWRLFQNPESLLFRVLKGRYFSRSDFLSAATCGVSSL 1328

Query: 301  TWRSILWGRDL 312
            TW+   WGR+L
Sbjct: 1329 TWQGFCWGREL 1339

BLAST of Lag0000286 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-31
Identity = 58/120 (48.33%), Postives = 78/120 (65.00%), Query Frame = 0

Query: 193 AIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCKSKA-LGGMDFWD 252
           A+P Y MSCF+L K  C  +  A   FWW S +  RKI W+ W +LCKSK   GG+ F D
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 253 ISLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSLTWRSILWGRDL 312
           +  FNQA+LAKQS++I+  P+ LLS++L  RYF H   +  ++G  PS  WRSI+ GR+L
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

BLAST of Lag0000286 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 3.3e-28
Identity = 60/138 (43.48%), Postives = 77/138 (55.80%), Query Frame = 0

Query: 193 AIPTYTMSCFKLPKFFCDDINRACARFWWVSSQGNRKIHWMNWHRLCKSKALGGMDFWDI 252
           A+PTYTM+CF LPK  C  I    A FWW + Q  + +HW  W  L   KA GG+ F DI
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 253 SLFNQAMLAKQSWKILKNPNCLLSKILMGRYFKHGDYLNATIGHNPSLTWRSILWGRDLF 312
             FN A+L KQ W++L  P  L++K+   RYF   D LNA +G  PS  W+SI   +++ 
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEI- 121

Query: 313 LAHSVRAYEQQNE--IIW 329
           L    RA     E  IIW
Sbjct: 122 LRQGARAVVGNGEDIIIW 138

BLAST of Lag0000286 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 73.9 bits (180), Expect = 3.0e-13
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 0

Query: 12 LVNGSPSETIKPERGLGQGDPLSPYLFLIRAEVLSSLLLREKSLSHFNGICINNLYPSIS 71
          ++NG+P   + P RGL QGDPLSPYLF++  EVLS L  R +      GI ++N  P I+
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 72 HLFFADD 79
          HL FADD
Sbjct: 73 HLLFADD 79