IVF0023880 (gene) Melon (IVF77) v1

Overview
NameIVF0023880
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionProcollagen-proline 4-dioxygenase
Locationchr04: 26269673 .. 26272810 (-)
RNA-Seq ExpressionIVF0023880
SyntenyIVF0023880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTTTCAGAGAGTCAATGCAAAGCAATTTCCCGCTGGAAGAAACTTCCATTTCCATGGTTATCAACGGCGGGTTTGCAATTGCAGCAAATCCGCTTCTGGGTTCCCTAAATTTCTTCATAAATGAGTATGAACCCCAAGAATCGAATTTGTACCTTCCTCTCTCTTCTCCATTTTCGTTCTTTGTTTTGATTTCCATTTTCTACATACATTTTGTTTACGTCTCCCTTTCTCTCTTGAATTTCTGGCTTCCGTACCTCCATTTTCGCTATGGCTTCTCCATTTCTTCTCGCATTTTCTATCTTTTTCCTTTGGCTTTTACCCCTTTCTTCTCTCTCTGCCAACCGCTTCCCCAAAATGCTCTTACACAACAACGACATGTAATAATACTTCATCGATTTCTTGATTTCTACTTTTCTTTTCTCTGTTTTCGTTTTCCCCATTCATACGGCATTTTAATATTTGACCTAATTGATGTCAATTTCACAGGTATGAATCTGTTATTAGGATGAAAACGGGTGGTTCCGCCATTACAATCGATCCCACTCGTGTCATTCAGCTTTCATCCAAACCCAGGTCCTTAAATTTCTTTCATTGGTTTTCCATTAGCTCTAAAACTTGCAGATTTTGGAGAACCCATTTTGAATTTTCACTTAATCTGATGAAAATGTACAGGGCTTTCTTATATAAGGGATTTTTGTCTTATGAGGAGTGCCAGCATCTTATCCATTTGGTAGAAGAAAATTCTTATTCTTCTTTTTTTTTGGAGAAAATTAATCACCAAACTCCATAGTGTATAATTGTGATACATATTGTGTAGGCGAAGGGTAAGCTACGTCAATCATTGGTGGCGGCTGGAACAGGTGAGAGTGTTACAAGTAAAGAACGGACGAGTACTGGCATGTTTCTTCGCAAGGCCCAGGTATTTTAACTCAATAGTAACAGAAGTTGATGTATCCATAATGTTATTTACTGGTCATCTACTCAAAATATCTCACTTTCTGTAGTTTAGACTGCCTTTTCCATTTTGGTTATCCTGTGGGTTTGGTTGAATTCGTATAAGGACAGAACATGAAACACGAACGAGAGAGTTAAGCATTGATGTTATGTGAAGGTGCTGGAAGAATCAAATATCTTGTCCTTATCTATCTTCGGCTGTTGCGAGCATAATCCTGCCTGTTTCATTTTTTGTTTTAATTGGTCGTGAAAAGGTCTTCTTTCAATTGGCTTTTGATATACTTTTTGGGTTAATAGACGGAAGAGCAATCCAAGAATCCAAGAGCTATGTAGTATGTTTGAGTTCTTACTTTGTTTTAGTCGAATGACAGTACTTTTACTAATCAGTCAAAATATAATTTCATATTAAGGTAAAATTAGTACCTTGTCTCTGCAGTTTTATATGCTAAACCCAATAGTCTCAACTTCAATGAATGATGTTGAGTGAAATTAAAATTTTAACTTGGTGTTGATGTTGATTGGCGTAAGGTTGATGTGATCAATGTCATAGTAGGATGGAGTTACAAGACCGAGAACTCTTAAACTTGAAATGCGATTTCCCTATTATTTTTTCCTGGCATTTGCAGGTTGAGGAACTTTTAGTTGTGGGTTATTTATTGGTAGATGTTGAGTTGTGTCGAAGTGCAGACAACCTTTCATTGTTTTTTTAGATTTGCATCTTTCTTAAATATAAACAATGTAGAGTTTGATTCTATGGTTCCATTCTCTTCCCAGGATAAAATAGTTGCTCGCATTGAGTCAAGGATTGCTGCATGGACTTTCCTTCCCCTTGGTAAACTATATAACTTATTTGATGTTTTGTCTTTCATTTATTTACGAAATGAGGCTTGTATAAACTCGAAATTGTTGTTCTGTTCAGATAATGGGGAGCCTATTCAGATACTAAGGTATGAGAACGGACAGAAATATGAGCCACATTTTGATTTTTTTCAAGACCCAGGCAATATAGCGATTGGAGGTCACCGGATAGCCACAATCTTGATGTATTTATCCGATGTTGAAAAGGGTGGAGAAACAGTCTTTCCCAATTCTCCGGTATTGCTTTTTTGAAACATTAACCGCTCTCAATCAGGACTATTTGTCATTATTGAGTTGTTATATTGTAACTATTAAACTTGCATGTTATGAATAATTCCATTTTCAAATCACTCTGGCAGGTTAAATTATCCGAGGAGGAAAAGGGTGACTTGTCTGAATGCGCTAAGGTTGGCTATGGAGGTATTGAATCTTTTCTTTGAAAATATATTATTGGAGGCTGCGTTGGCACTGAGTTAATGCTGCAATATGAGAATCTCATAATTCCCTAAACATTTCCTAATATCTTTTGTCTCAACTTTTGGTCAATGACTTGGAGATCTGTTGATTGGTAATTCTTCAAATCAAAAAGCCAACTGGAAAAATTTGTTTATTCAAAAGGACTGCCTATGTTCTATCAGTTGTTCAATTCTTACATTAGAAATACGATGCTGAAAACATTACTAAGAATTTTGACATAAAGCATATAAATATAAAGTGGTTGATAATCAACTCCCGAGTTTGCGGAAAAACTGCCATGAGAAAAAGCATCATCTTAATTGTCTCTACATCACATTTTTCTCTCGTTCCAGCTCATTGTTTCTTCAGCAATCTGTTATTGTAACGTTCTGCAATCAAATTTGTATGATTCAAAAGATTACGTTTGTTTGTGGGTGCAGTAAGACCAAAGTTGGGTGATGCTTTACTGTTCTTCAGTATGAATCCAAATGTGACACCAGACGCGACCAGCTATCACGGGAGCTGCCCAGTGATAGAGGGTGAGAAATGGTCTGCAACTAAATGGATTCACATGCTTCCAATCGATGAAGTTTGGAGGAATCCAGCTTGTGTAGACGAAAATGACCACTGTAGTGCGTGGGCAAAAGCAGGTGAATGTAAAAAGAATCCTGTTTATATGATGGGTTCTAAGAATGAACTTGGATTTTGTAGGTTGAGTTGCAAAGTATGCTCTCCTTCCTCATAGAAAAGGAAATGCTTGTTTTTACTTATACACAATTCAGTGGTAGGAGTTTTTTCTTCTTACACATGTACACATGTAAATGACTTTGAGAGGCATCACTTAGTATATTTAACTATTGAATCTCTC

mRNA sequence

GCTTTTCAGAGAGTCAATGCAAAGCAATTTCCCGCTGGAAGAAACTTCCATTTCCATGGTTATCAACGGCGGGTTTGCAATTGCAGCAAATCCGCTTCTGGGTTCCCTAAATTTCTTCATAAATGAGTATGAACCCCAAGAATCGAATTTGTACCTTCCTCTCTCTTCTCCATTTTCGTTCTTTGTTTTGATTTCCATTTTCTACATACATTTTGTTTACGTCTCCCTTTCTCTCTTGAATTTCTGGCTTCCGTACCTCCATTTTCGCTATGGCTTCTCCATTTCTTCTCGCATTTTCTATCTTTTTCCTTTGGCTTTTACCCCTTTCTTCTCTCTCTGCCAACCGCTTCCCCAAAATGCTCTTACACAACAACGACATGTATGAATCTGTTATTAGGATGAAAACGGGTGGTTCCGCCATTACAATCGATCCCACTCGTGTCATTCAGCTTTCATCCAAACCCAGGGCTTTCTTATATAAGGGATTTTTGTCTTATGAGGAGTGCCAGCATCTTATCCATTTGGCGAAGGGTAAGCTACGTCAATCATTGGTGGCGGCTGGAACAGGTGAGAGTGTTACAAGTAAAGAACGGACGAGTACTGGCATGTTTCTTCGCAAGGCCCAGGATAAAATAGTTGCTCGCATTGAGTCAAGGATTGCTGCATGGACTTTCCTTCCCCTTGATAATGGGGAGCCTATTCAGATACTAAGGTATGAGAACGGACAGAAATATGAGCCACATTTTGATTTTTTTCAAGACCCAGGCAATATAGCGATTGGAGGTCACCGGATAGCCACAATCTTGATGTATTTATCCGATGTTGAAAAGGGTGGAGAAACAGTCTTTCCCAATTCTCCGGTTAAATTATCCGAGGAGGAAAAGGGTGACTTGTCTGAATGCGCTAAGGTTGGCTATGGAGTAAGACCAAAGTTGGGTGATGCTTTACTGTTCTTCAGTATGAATCCAAATGTGACACCAGACGCGACCAGCTATCACGGGAGCTGCCCAGTGATAGAGGGTGAGAAATGGTCTGCAACTAAATGGATTCACATGCTTCCAATCGATGAAGTTTGGAGGAATCCAGCTTGTGTAGACGAAAATGACCACTGTAGTGCGTGGGCAAAAGCAGGTGAATGTAAAAAGAATCCTGTTTATATGATGGGTTCTAAGAATGAACTTGGATTTTGTAGGTTGAGTTGCAAAGTATGCTCTCCTTCCTCATAGAAAAGGAAATGCTTGTTTTTACTTATACACAATTCAGTGGTAGGAGTTTTTTCTTCTTACACATGTACACATGTAAATGACTTTGAGAGGCATCACTTAGTATATTTAACTATTGAATCTCTC

Coding sequence (CDS)

ATGGCTTCTCCATTTCTTCTCGCATTTTCTATCTTTTTCCTTTGGCTTTTACCCCTTTCTTCTCTCTCTGCCAACCGCTTCCCCAAAATGCTCTTACACAACAACGACATGTATGAATCTGTTATTAGGATGAAAACGGGTGGTTCCGCCATTACAATCGATCCCACTCGTGTCATTCAGCTTTCATCCAAACCCAGGGCTTTCTTATATAAGGGATTTTTGTCTTATGAGGAGTGCCAGCATCTTATCCATTTGGCGAAGGGTAAGCTACGTCAATCATTGGTGGCGGCTGGAACAGGTGAGAGTGTTACAAGTAAAGAACGGACGAGTACTGGCATGTTTCTTCGCAAGGCCCAGGATAAAATAGTTGCTCGCATTGAGTCAAGGATTGCTGCATGGACTTTCCTTCCCCTTGATAATGGGGAGCCTATTCAGATACTAAGGTATGAGAACGGACAGAAATATGAGCCACATTTTGATTTTTTTCAAGACCCAGGCAATATAGCGATTGGAGGTCACCGGATAGCCACAATCTTGATGTATTTATCCGATGTTGAAAAGGGTGGAGAAACAGTCTTTCCCAATTCTCCGGTTAAATTATCCGAGGAGGAAAAGGGTGACTTGTCTGAATGCGCTAAGGTTGGCTATGGAGTAAGACCAAAGTTGGGTGATGCTTTACTGTTCTTCAGTATGAATCCAAATGTGACACCAGACGCGACCAGCTATCACGGGAGCTGCCCAGTGATAGAGGGTGAGAAATGGTCTGCAACTAAATGGATTCACATGCTTCCAATCGATGAAGTTTGGAGGAATCCAGCTTGTGTAGACGAAAATGACCACTGTAGTGCGTGGGCAAAAGCAGGTGAATGTAAAAAGAATCCTGTTTATATGATGGGTTCTAAGAATGAACTTGGATTTTGTAGGTTGAGTTGCAAAGTATGCTCTCCTTCCTCATAG

Protein sequence

MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS
Homology
BLAST of IVF0023880 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 7.2e-106
Identity = 187/317 (58.99%), Postives = 233/317 (73.50%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           M S   LAFS+ FL+ LPL S + NRF  +   +N    SVI+MKT  S+   DPTRV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRF--LTRSSNTRDGSVIKMKTSASSFGFDPTRVTQ 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKERTSTGMFLRKAQ 120
           LS  PR FLY+GFLS EEC H I LAKGKL +S+VA   +GESV S+ RTS+GMFL K Q
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 120

Query: 121 DKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATIL 180
           D IV+ +E+++AAWTFLP +NGE +QIL YENGQKYEPHFD+F D  N+ +GGHRIAT+L
Sbjct: 121 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 180

Query: 181 MYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDA 240
           MYLS+VEKGGETVFP    K ++ +    +ECAK GY V+P+ GDALLFF+++PN T D+
Sbjct: 181 MYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDS 240

Query: 241 TSYHGSCPVIEGEKWSATKWIHMLPIDEVW-RNPACVDENDHCSAWAKAGECKKNPVYMM 300
            S HGSCPV+EGEKWSAT+WIH+   +  + +   C+DEN  C  WAKAGEC+KNP YM+
Sbjct: 241 NSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMV 300

Query: 301 GSKNELGFCRLSCKVCS 316
           GS  + G+CR SCK CS
Sbjct: 301 GSDKDHGYCRKSCKACS 315

BLAST of IVF0023880 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.4e-95
Identity = 163/267 (61.05%), Postives = 203/267 (76.03%), Query Frame = 0

Query: 50  AITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA--GTGESVTSKE 109
           + ++DPTR+ QLS  PRAFLYKGFLS EEC HLI LAKGKL +S+V A   +GES  S+ 
Sbjct: 24  SFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEV 83

Query: 110 RTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGN 169
           RTS+GMFL K QD IVA +E+++AAWTFLP +NGE +QIL YENGQKY+PHFD+F D   
Sbjct: 84  RTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKA 143

Query: 170 IAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALL 229
           + +GGHRIAT+LMYLS+V KGGETVFPN   K  + +    S+CAK GY V+P+ GDALL
Sbjct: 144 LELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALL 203

Query: 230 FFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKA 289
           FF+++ N T D  S HGSCPVIEGEKWSAT+WIH+    +  +   CVD+++ C  WA A
Sbjct: 204 FFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGK--KKLVCVDDHESCQEWADA 263

Query: 290 GECKKNPVYMMGSKNELGFCRLSCKVC 315
           GEC+KNP+YM+GS+  LGFCR SCK C
Sbjct: 264 GECEKNPMYMVGSETSLGFCRKSCKAC 288

BLAST of IVF0023880 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 9.8e-87
Identity = 151/271 (55.72%), Postives = 198/271 (73.06%), Query Frame = 0

Query: 49  SAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKE 108
           S++ ++P++V Q+SSKPRAF+Y+GFL+  EC H++ LAK  L++S VA   +GES  S+ 
Sbjct: 28  SSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEV 87

Query: 109 RTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGN 168
           RTS+G F+ K +D IV+ IE +I+ WTFLP +NGE IQ+LRYE+GQKY+ HFD+F D  N
Sbjct: 88  RTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVN 147

Query: 169 IAIGGHRIATILMYLSDVEKGGETVFPNSPV---KLSEEEKGDLSECAKVGYGVRPKLGD 228
           I  GGHR+ATILMYLS+V KGGETVFP++ +   ++  E K DLS+CAK G  V+P+ GD
Sbjct: 148 IVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGD 207

Query: 229 ALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDE-VWRNPACVDENDHCSA 288
           ALLFF+++P+  PD  S HG CPVIEGEKWSATKWIH+   D  V  +  C D N+ C  
Sbjct: 208 ALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSGNCTDMNESCER 267

Query: 289 WAKAGECKKNPVYMMGSKNELGFCRLSCKVC 315
           WA  GEC KNP YM+G+    G+CR SCK C
Sbjct: 268 WAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

BLAST of IVF0023880 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-83
Identity = 149/267 (55.81%), Postives = 190/267 (71.16%), Query Frame = 0

Query: 53  IDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKERTST 112
           I+P++V Q+SSKPRAF+Y+GFL+  EC HLI LAK  L++S VA    GES  S  RTS+
Sbjct: 33  INPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSS 92

Query: 113 GMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIG 172
           G F+ K +D IV+ IE +++ WTFLP +NGE +Q+LRYE+GQKY+ HFD+F D  NIA G
Sbjct: 93  GTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARG 152

Query: 173 GHRIATILMYLSDVEKGGETVFPNSPV---KLSEEEKGDLSECAKVGYGVRPKLGDALLF 232
           GHRIAT+L+YLS+V KGGETVFP++     +   E K DLS+CAK G  V+PK G+ALLF
Sbjct: 153 GHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLF 212

Query: 233 FSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPA-CVDENDHCSAWAKA 292
           F++  +  PD  S HG CPVIEGEKWSATKWIH+   D++  +   C D N+ C  WA  
Sbjct: 213 FNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDGNCTDVNESCERWAVL 272

Query: 293 GECKKNPVYMMGSKNELGFCRLSCKVC 315
           GEC KNP YM+G+    G CR SCK C
Sbjct: 273 GECGKNPEYMVGTPEIPGNCRRSCKAC 299

BLAST of IVF0023880 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.9e-62
Identity = 115/204 (56.37%), Postives = 151/204 (74.02%), Query Frame = 0

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKL-RQSLVAAGTGESVTSKERTSTGMFLRKAQ 120
           LS +PRAF+Y  FLS EEC++LI LAK  + + ++V + TG+S  S+ RTS+G FLR+ +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 121 DKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATIL 180
           DKI+  IE RIA +TF+P D+GE +Q+L YE GQKYEPH+D+F D  N   GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 181 MYLSDVEKGGETVFPNSPVKLSEEE-KGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPD 240
           MYLSDVE+GGETVFP + +  S      +LSEC K G  V+P++GDALLF+SM P+ T D
Sbjct: 199 MYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLD 258

Query: 241 ATSYHGSCPVIEGEKWSATKWIHM 263
            TS HG CPVI G KWS+TKW+H+
Sbjct: 259 PTSLHGGCPVIRGNKWSSTKWMHV 282

BLAST of IVF0023880 vs. ExPASy TrEMBL
Match: A0A1S3B814 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103487037 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.5e-186
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ
Sbjct: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD 120
           LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD
Sbjct: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD 120

Query: 121 KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM 180
           KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM
Sbjct: 121 KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM 180

Query: 181 YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT 240
           YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT
Sbjct: 181 YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT 240

Query: 241 SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS 300
           SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS
Sbjct: 241 SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS 300

Query: 301 KNELGFCRLSCKVCSPSS 319
           KNELGFCRLSCKVCSPSS
Sbjct: 301 KNELGFCRLSCKVCSPSS 318

BLAST of IVF0023880 vs. ExPASy TrEMBL
Match: A0A0A0LG32 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G828960 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.1e-170
Identity = 293/319 (91.85%), Postives = 304/319 (95.30%), Query Frame = 0

Query: 1   MASPFLLAFSIF--FLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRV 60
           MASPF L FSIF  FL+LLP SSLSANRFPK++LHNND+ ESVIRMKTGGSA+TIDPTRV
Sbjct: 1   MASPFFLPFSIFFLFLFLLPFSSLSANRFPKLILHNNDIDESVIRMKTGGSAMTIDPTRV 60

Query: 61  IQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKA 120
           IQLSSKPRAFLYKGFLS EECQHLI+ AKGKL QSLVAAGTG+SVTSKERTSTGMFL KA
Sbjct: 61  IQLSSKPRAFLYKGFLSAEECQHLINSAKGKLHQSLVAAGTGQSVTSKERTSTGMFLHKA 120

Query: 121 QDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATI 180
           QD+IVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATI
Sbjct: 121 QDEIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATI 180

Query: 181 LMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPD 240
           LMYLS+VEKGGETVFPNSPVKLSEEEK DLSEC KVGYGVRPKLGDALLFFSMNPNVTPD
Sbjct: 181 LMYLSNVEKGGETVFPNSPVKLSEEEKADLSECGKVGYGVRPKLGDALLFFSMNPNVTPD 240

Query: 241 ATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMM 300
            TSYHGSCPVIEGEKWSATKWIHMLPIDE WRNPACVDENDHC+AWAKAGEC+KNPVYMM
Sbjct: 241 TTSYHGSCPVIEGEKWSATKWIHMLPIDEFWRNPACVDENDHCTAWAKAGECEKNPVYMM 300

Query: 301 GSKNELGFCRLSCKVCSPS 318
           GSKNELGFCR SCKVCSPS
Sbjct: 301 GSKNELGFCRFSCKVCSPS 319

BLAST of IVF0023880 vs. ExPASy TrEMBL
Match: A0A5D3D1X2 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00060 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.5e-165
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 38  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 97
           YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA
Sbjct: 44  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 103

Query: 98  GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEP 157
           GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEP
Sbjct: 104 GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEP 163

Query: 158 HFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYG 217
           HFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYG
Sbjct: 164 HFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYG 223

Query: 218 VRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDE 277
           VRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDE
Sbjct: 224 VRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDE 283

Query: 278 NDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS 319
           NDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS
Sbjct: 284 NDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS 324

BLAST of IVF0023880 vs. ExPASy TrEMBL
Match: A0A5A7UCT9 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00990 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 281/310 (90.65%), Postives = 281/310 (90.65%), Query Frame = 0

Query: 38  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 97
           YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA
Sbjct: 44  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 103

Query: 98  GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPL------------------- 157
           GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPL                   
Sbjct: 104 GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLGKLYNLFDVLSFIYLRNEA 163

Query: 158 ----------DNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKG 217
                     DNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKG
Sbjct: 164 CINSKLLFCSDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKG 223

Query: 218 GETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPV 277
           GETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPV
Sbjct: 224 GETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPV 283

Query: 278 IEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCR 319
           IEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCR
Sbjct: 284 IEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCR 343

BLAST of IVF0023880 vs. ExPASy TrEMBL
Match: A0A6J1EYJ1 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437385 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.3e-134
Identity = 237/323 (73.37%), Postives = 268/323 (82.97%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           M S F LAFS+ FL   PL + SANR PK+LL +    +SVIRMK  GS+I IDPTRV+Q
Sbjct: 1   MDSRFFLAFSLCFLCSFPLFARSANRLPKLLLDDTKTEDSVIRMKMDGSSIKIDPTRVVQ 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKERTSTGMFLRKAQ 120
           LSS+PRAFLYKGFLS EECQHLI LAK  L QSLV    TG S +S +RTSTGMFL KAQ
Sbjct: 61  LSSQPRAFLYKGFLSAEECQHLIDLAKDYLEQSLVVDDITGASRSSTDRTSTGMFLYKAQ 120

Query: 121 DKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATIL 180
           D IVA IE++IAAWTFLP+DNGEPIQILRYENGQ+Y PHFDFFQDP N+A GGHRIAT+L
Sbjct: 121 DDIVAGIEAKIAAWTFLPVDNGEPIQILRYENGQQYVPHFDFFQDPVNVAAGGHRIATVL 180

Query: 181 MYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDA 240
           MYLS+VE+GGETVFP+SP K+ EEE  DL +C+  GYGV+PK GDALLFFS++PNVT D 
Sbjct: 181 MYLSNVERGGETVFPDSPAKVFEEENKDLFDCSTTGYGVKPKKGDALLFFSLHPNVTTDP 240

Query: 241 TSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYM-- 300
           TSYHGSCPVIEGEKWSATKWIHMLP+DE+WRNP CVDEN+HCSAWAKAGEC+KNP YM  
Sbjct: 241 TSYHGSCPVIEGEKWSATKWIHMLPVDEIWRNPDCVDENEHCSAWAKAGECEKNPGYMVG 300

Query: 301 --MGSKNELGFCRLSCKVCSPSS 319
             +GSK ELG+CRLSCK CSP S
Sbjct: 301 SSLGSKEELGYCRLSCKACSPPS 323

BLAST of IVF0023880 vs. NCBI nr
Match: XP_008443446.1 (PREDICTED: probable prolyl 4-hydroxylase 7 [Cucumis melo])

HSP 1 Score: 653 bits (1685), Expect = 8.04e-237
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ
Sbjct: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD 120
           LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD
Sbjct: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD 120

Query: 121 KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM 180
           KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM
Sbjct: 121 KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM 180

Query: 181 YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT 240
           YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT
Sbjct: 181 YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT 240

Query: 241 SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS 300
           SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS
Sbjct: 241 SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS 300

Query: 301 KNELGFCRLSCKVCSPSS 318
           KNELGFCRLSCKVCSPSS
Sbjct: 301 KNELGFCRLSCKVCSPSS 318

BLAST of IVF0023880 vs. NCBI nr
Match: XP_004147455.1 (probable prolyl 4-hydroxylase 7 [Cucumis sativus] >KGN59607.1 hypothetical protein Csa_001214 [Cucumis sativus])

HSP 1 Score: 601 bits (1549), Expect = 4.50e-216
Identity = 293/319 (91.85%), Postives = 304/319 (95.30%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWL--LPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRV 60
           MASPF L FSIFFL+L  LP SSLSANRFPK++LHNND+ ESVIRMKTGGSA+TIDPTRV
Sbjct: 1   MASPFFLPFSIFFLFLFLLPFSSLSANRFPKLILHNNDIDESVIRMKTGGSAMTIDPTRV 60

Query: 61  IQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKA 120
           IQLSSKPRAFLYKGFLS EECQHLI+ AKGKL QSLVAAGTG+SVTSKERTSTGMFL KA
Sbjct: 61  IQLSSKPRAFLYKGFLSAEECQHLINSAKGKLHQSLVAAGTGQSVTSKERTSTGMFLHKA 120

Query: 121 QDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATI 180
           QD+IVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATI
Sbjct: 121 QDEIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATI 180

Query: 181 LMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPD 240
           LMYLS+VEKGGETVFPNSPVKLSEEEK DLSEC KVGYGVRPKLGDALLFFSMNPNVTPD
Sbjct: 181 LMYLSNVEKGGETVFPNSPVKLSEEEKADLSECGKVGYGVRPKLGDALLFFSMNPNVTPD 240

Query: 241 ATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMM 300
            TSYHGSCPVIEGEKWSATKWIHMLPIDE WRNPACVDENDHC+AWAKAGEC+KNPVYMM
Sbjct: 241 TTSYHGSCPVIEGEKWSATKWIHMLPIDEFWRNPACVDENDHCTAWAKAGECEKNPVYMM 300

Query: 301 GSKNELGFCRLSCKVCSPS 317
           GSKNELGFCR SCKVCSPS
Sbjct: 301 GSKNELGFCRFSCKVCSPS 319

BLAST of IVF0023880 vs. NCBI nr
Match: TYK17735.1 (putative prolyl 4-hydroxylase 7 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 585 bits (1508), Expect = 9.61e-210
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 38  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 97
           YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA
Sbjct: 44  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 103

Query: 98  GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEP 157
           GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEP
Sbjct: 104 GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEP 163

Query: 158 HFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYG 217
           HFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYG
Sbjct: 164 HFDFFQDPGNIAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYG 223

Query: 218 VRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDE 277
           VRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDE
Sbjct: 224 VRPKLGDALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDE 283

Query: 278 NDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS 318
           NDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS
Sbjct: 284 NDHCSAWAKAGECKKNPVYMMGSKNELGFCRLSCKVCSPSS 324

BLAST of IVF0023880 vs. NCBI nr
Match: KAA0053723.1 (putative prolyl 4-hydroxylase 7 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 570 bits (1468), Expect = 3.48e-203
Identity = 281/310 (90.65%), Postives = 281/310 (90.65%), Query Frame = 0

Query: 38  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 97
           YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA
Sbjct: 44  YESVIRMKTGGSAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA 103

Query: 98  GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPL------------------- 157
           GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPL                   
Sbjct: 104 GTGESVTSKERTSTGMFLRKAQDKIVARIESRIAAWTFLPLGKLYNLFDVLSFIYLRNEA 163

Query: 158 ----------DNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKG 217
                     DNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKG
Sbjct: 164 CINSKLLFCSDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILMYLSDVEKG 223

Query: 218 GETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPV 277
           GETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPV
Sbjct: 224 GETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDATSYHGSCPV 283

Query: 278 IEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCR 318
           IEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCR
Sbjct: 284 IEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGSKNELGFCR 343

BLAST of IVF0023880 vs. NCBI nr
Match: XP_038905408.1 (probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida] >XP_038905409.1 probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida])

HSP 1 Score: 559 bits (1441), Expect = 1.25e-199
Identity = 270/318 (84.91%), Postives = 293/318 (92.14%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           MAS F LAFS+ FL   P  S SANR PK+LLHNN+M +SVIRMKT GS +TIDPTRVI+
Sbjct: 1   MASRFFLAFSLCFLCFFPFFSRSANRLPKLLLHNNNMDQSVIRMKTVGSPVTIDPTRVIK 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAGTGESVTSKERTSTGMFLRKAQD 120
           LSSKPRAFLYKGFLS +ECQHLI+LAKGKL+QSLVAA TGESVTS+ERTSTGMFL +AQD
Sbjct: 61  LSSKPRAFLYKGFLSEDECQHLINLAKGKLQQSLVAAETGESVTSQERTSTGMFLTRAQD 120

Query: 121 KIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATILM 180
           +IVARIESRIAAWTFLP+DNGEPIQILRYENGQKYEPHFDFFQDP NIAIGGHRIATILM
Sbjct: 121 EIVARIESRIAAWTFLPIDNGEPIQILRYENGQKYEPHFDFFQDPVNIAIGGHRIATILM 180

Query: 181 YLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDAT 240
           YLSDVEKGGETVFPNSP+KLSE+E+ DLS+CAKVGYGV+PK+GDALLFFS+NPNVTPDAT
Sbjct: 181 YLSDVEKGGETVFPNSPIKLSEQERADLSDCAKVGYGVKPKMGDALLFFSLNPNVTPDAT 240

Query: 241 SYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKAGECKKNPVYMMGS 300
           SYHGSCPVIEGEKWSATKWIHMLPI E+WRNPACVDEN  C AWA AGEC+KNPVYMMGS
Sbjct: 241 SYHGSCPVIEGEKWSATKWIHMLPIYEIWRNPACVDENVQCRAWANAGECEKNPVYMMGS 300

Query: 301 KNELGFCRLSCKVCSPSS 318
           KNELG CR+SCKVCSP S
Sbjct: 301 KNELGHCRMSCKVCSPPS 318

BLAST of IVF0023880 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 385.2 bits (988), Expect = 5.1e-107
Identity = 187/317 (58.99%), Postives = 233/317 (73.50%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           M S   LAFS+ FL+ LPL S + NRF  +   +N    SVI+MKT  S+   DPTRV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRF--LTRSSNTRDGSVIKMKTSASSFGFDPTRVTQ 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKERTSTGMFLRKAQ 120
           LS  PR FLY+GFLS EEC H I LAKGKL +S+VA   +GESV S+ RTS+GMFL K Q
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 120

Query: 121 DKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIGGHRIATIL 180
           D IV+ +E+++AAWTFLP +NGE +QIL YENGQKYEPHFD+F D  N+ +GGHRIAT+L
Sbjct: 121 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 180

Query: 181 MYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSMNPNVTPDA 240
           MYLS+VEKGGETVFP    K ++ +    +ECAK GY V+P+ GDALLFF+++PN T D+
Sbjct: 181 MYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDS 240

Query: 241 TSYHGSCPVIEGEKWSATKWIHMLPIDEVW-RNPACVDENDHCSAWAKAGECKKNPVYMM 300
            S HGSCPV+EGEKWSAT+WIH+   +  + +   C+DEN  C  WAKAGEC+KNP YM+
Sbjct: 241 NSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMV 300

Query: 301 GSKNELGFCRLSCKVCS 316
           GS  + G+CR SCK CS
Sbjct: 301 GSDKDHGYCRKSCKACS 315

BLAST of IVF0023880 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 361.7 bits (927), Expect = 6.1e-100
Identity = 180/325 (55.38%), Postives = 228/325 (70.15%), Query Frame = 0

Query: 1   MASPFLLAFSIFFLWLLPLSSLSANRFPKMLLHNNDMYESVIRMKTGGSAITIDPTRVIQ 60
           M S   LAFS+ FL+ LPL S + NRF  +   +N    SVI+MKT  S+   DPTRV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRF--LTRSSNTRDGSVIKMKTSASSFGFDPTRVTQ 60

Query: 61  LSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKERTS----TGMFL 120
           LS  PR FLY+GFLS EEC H I LAKGKL +S+VA   +GESV S++  S    +  F+
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFI 120

Query: 121 RKAQ----DKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIG 180
                   D IV+ +E+++AAWTFLP +NGE +QIL YENGQKYEPHFD+F D  N+ +G
Sbjct: 121 ANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELG 180

Query: 181 GHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALLFFSM 240
           GHRIAT+LMYLS+VEKGGETVFP    K ++ +    +ECAK GY V+P+ GDALLFF++
Sbjct: 181 GHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNL 240

Query: 241 NPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVW-RNPACVDENDHCSAWAKAGEC 300
           +PN T D+ S HGSCPV+EGEKWSAT+WIH+   +  + +   C+DEN  C  WAKAGEC
Sbjct: 241 HPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGEC 300

Query: 301 KKNPVYMMGSKNELGFCRLSCKVCS 316
           +KNP YM+GS  + G+CR SCK CS
Sbjct: 301 QKNPTYMVGSDKDHGYCRKSCKACS 323

BLAST of IVF0023880 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 349.7 bits (896), Expect = 2.4e-96
Identity = 163/267 (61.05%), Postives = 203/267 (76.03%), Query Frame = 0

Query: 50  AITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAA--GTGESVTSKE 109
           + ++DPTR+ QLS  PRAFLYKGFLS EEC HLI LAKGKL +S+V A   +GES  S+ 
Sbjct: 24  SFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEV 83

Query: 110 RTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGN 169
           RTS+GMFL K QD IVA +E+++AAWTFLP +NGE +QIL YENGQKY+PHFD+F D   
Sbjct: 84  RTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKA 143

Query: 170 IAIGGHRIATILMYLSDVEKGGETVFPNSPVKLSEEEKGDLSECAKVGYGVRPKLGDALL 229
           + +GGHRIAT+LMYLS+V KGGETVFPN   K  + +    S+CAK GY V+P+ GDALL
Sbjct: 144 LELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALL 203

Query: 230 FFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPACVDENDHCSAWAKA 289
           FF+++ N T D  S HGSCPVIEGEKWSAT+WIH+    +  +   CVD+++ C  WA A
Sbjct: 204 FFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGK--KKLVCVDDHESCQEWADA 263

Query: 290 GECKKNPVYMMGSKNELGFCRLSCKVC 315
           GEC+KNP+YM+GS+  LGFCR SCK C
Sbjct: 264 GECEKNPMYMVGSETSLGFCRKSCKAC 288

BLAST of IVF0023880 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 7.0e-88
Identity = 151/271 (55.72%), Postives = 198/271 (73.06%), Query Frame = 0

Query: 49  SAITIDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKE 108
           S++ ++P++V Q+SSKPRAF+Y+GFL+  EC H++ LAK  L++S VA   +GES  S+ 
Sbjct: 28  SSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEV 87

Query: 109 RTSTGMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGN 168
           RTS+G F+ K +D IV+ IE +I+ WTFLP +NGE IQ+LRYE+GQKY+ HFD+F D  N
Sbjct: 88  RTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVN 147

Query: 169 IAIGGHRIATILMYLSDVEKGGETVFPNSPV---KLSEEEKGDLSECAKVGYGVRPKLGD 228
           I  GGHR+ATILMYLS+V KGGETVFP++ +   ++  E K DLS+CAK G  V+P+ GD
Sbjct: 148 IVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGD 207

Query: 229 ALLFFSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDE-VWRNPACVDENDHCSA 288
           ALLFF+++P+  PD  S HG CPVIEGEKWSATKWIH+   D  V  +  C D N+ C  
Sbjct: 208 ALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSGNCTDMNESCER 267

Query: 289 WAKAGECKKNPVYMMGSKNELGFCRLSCKVC 315
           WA  GEC KNP YM+G+    G+CR SCK C
Sbjct: 268 WAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

BLAST of IVF0023880 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 310.5 bits (794), Expect = 1.6e-84
Identity = 149/267 (55.81%), Postives = 190/267 (71.16%), Query Frame = 0

Query: 53  IDPTRVIQLSSKPRAFLYKGFLSYEECQHLIHLAKGKLRQSLVAAG-TGESVTSKERTST 112
           I+P++V Q+SSKPRAF+Y+GFL+  EC HLI LAK  L++S VA    GES  S  RTS+
Sbjct: 33  INPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSS 92

Query: 113 GMFLRKAQDKIVARIESRIAAWTFLPLDNGEPIQILRYENGQKYEPHFDFFQDPGNIAIG 172
           G F+ K +D IV+ IE +++ WTFLP +NGE +Q+LRYE+GQKY+ HFD+F D  NIA G
Sbjct: 93  GTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARG 152

Query: 173 GHRIATILMYLSDVEKGGETVFPNSPV---KLSEEEKGDLSECAKVGYGVRPKLGDALLF 232
           GHRIAT+L+YLS+V KGGETVFP++     +   E K DLS+CAK G  V+PK G+ALLF
Sbjct: 153 GHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLF 212

Query: 233 FSMNPNVTPDATSYHGSCPVIEGEKWSATKWIHMLPIDEVWRNPA-CVDENDHCSAWAKA 292
           F++  +  PD  S HG CPVIEGEKWSATKWIH+   D++  +   C D N+ C  WA  
Sbjct: 213 FNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDKILTHDGNCTDVNESCERWAVL 272

Query: 293 GECKKNPVYMMGSKNELGFCRLSCKVC 315
           GEC KNP YM+G+    G CR SCK C
Sbjct: 273 GECGKNPEYMVGTPEIPGNCRRSCKAC 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L9707.2e-10658.99Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
F4J0A83.4e-9561.05Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=... [more]
Q8LAN39.8e-8755.72Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
F4JAU32.3e-8355.81Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1[more]
Q9LN202.9e-6256.37Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3B8142.5e-186100.00Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103487037 PE=3 S... [more]
A0A0A0LG321.1e-17091.85Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G828960 PE=... [more]
A0A5D3D1X22.5e-165100.00Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7UCT91.1e-16090.65Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1EYJ11.3e-13473.37Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437385 ... [more]
Match NameE-valueIdentityDescription
XP_008443446.18.04e-237100.00PREDICTED: probable prolyl 4-hydroxylase 7 [Cucumis melo][more]
XP_004147455.14.50e-21691.85probable prolyl 4-hydroxylase 7 [Cucumis sativus] >KGN59607.1 hypothetical prote... [more]
TYK17735.19.61e-210100.00putative prolyl 4-hydroxylase 7 isoform X2 [Cucumis melo var. makuwa][more]
KAA0053723.13.48e-20390.65putative prolyl 4-hydroxylase 7 isoform X2 [Cucumis melo var. makuwa][more]
XP_038905408.11.25e-19984.91probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida] >XP_038905409.1 p... [more]
Match NameE-valueIdentityDescription
AT3G28480.15.1e-10758.99Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.26.1e-10055.38Oxoglutarate/iron-dependent oxygenase [more]
AT3G28490.12.4e-9661.05Oxoglutarate/iron-dependent oxygenase [more]
AT5G18900.17.0e-8855.722-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G06300.11.6e-8455.81P4H isoform 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 273..315
e-value: 1.6E-8
score: 44.3
IPR003582ShKT domainPFAMPF01549ShKcoord: 274..314
e-value: 3.9E-4
score: 20.9
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 274..314
score: 7.851336
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 65..261
e-value: 4.4E-55
score: 199.0
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 57..262
e-value: 1.6E-70
score: 239.0
NoneNo IPR availablePANTHERPTHR10869:SF140OS03G0803500 PROTEINcoord: 44..315
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 145..261
e-value: 1.7E-19
score: 70.5
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 44..315
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 140..262
score: 11.755658

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0023880.1IVF0023880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen