IVF0022385 (gene) Melon (IVF77) v1

BLAST of IVF0022385 vs. ExPASy Swiss-Prot
Match: Q9LFC0 (E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAKAI PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 9.4e-86
Identity = 194/384 (50.52%), Postives = 243/384 (63.28%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRL +    E+G GA +P P E+VTVACPDHLVLADLPVAKGIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNGA-RPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRL PCDHAFCL+CARSDS+CYLCD+RIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYLCDERIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSA-KQSTASEST 180
           EGIFICAAPHCL+SFLK+ +FE+H+H+ H  LL+ +A+KEDGN+ +  S  +QS+ASEST
Sbjct: 121 EGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASEST 180

Query: 181 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 240
           +R PLR  +                 QQSR+  RS    K    F Q QN   +S  D+ 
Sbjct: 181 LRAPLRSQL-----------------QQSRELNRSASFAKSQSGFSQVQNYPPDS--DNS 240

Query: 241 HSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYP-PLQPIPPPNY 300
              GF+                             + GI     +  YP P+  + PP+ 
Sbjct: 241 RPPGFET-------------------------ASPKPGI----RFPDYPQPMNLMQPPSL 300

Query: 301 VVPANSNPMLTPPLPFGYPPFPIE---GAQQYYN-TPYEVSRQDTAAETGSEQGSLLGFP 360
            VP N NP L  P  FG+P +P      +QQ++N   YE++R ++    GSEQ SLLG+P
Sbjct: 301 PVPMNQNPGL--PQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYP 330

Query: 361 PGAP-GGMNFSATY-PQSWNTGQA 377
           P +P   +NF  +Y P SWN G A
Sbjct: 361 PPSPMTNLNFQGSYPPPSWNPGMA 330

BLAST of IVF0022385 vs. ExPASy Swiss-Prot
Match: Q4V7X9 (E3 ubiquitin-protein ligase Hakai OS=Xenopus laevis OX=8355 GN=cbll1 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 9.7e-06
Identity = 98/400 (24.50%), Postives = 146/400 (36.50%), Query Frame = 0

Query: 70  VHFCVRCDFPIAIYGRLSPCDHAFCLDCA-----RSDSLC--------------YLCDDR 129
           VHFC +C  PI IYGR+ PC H FC DCA     ++D LC                C+D 
Sbjct: 101 VHFCDKCGLPIKIYGRMIPCKHVFCYDCALMHEKKADKLCPGTLVEDSTDTFKRMSCNDP 160

Query: 130 IQKIQTIKLMEGIFICA-APHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANS 189
           +Q+I+       +F+C+    C +++L + + ++HI+  H    KP A  +         
Sbjct: 161 VQRIEQC-ARGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRASKPTARPQ--------- 220

Query: 190 AKQSTASESTVRGPLRPLIS--PGSNSQPQERDEKFHR--QQSRDQPRSGMQQKQTPSFG 249
                     +  PL P  +  P     P ++    H   +Q    P   MQ      F 
Sbjct: 221 -------PEPIHPPLAPPPAEIPDRFIMPPDKHHLSHMPPKQHILMPPPPMQHVPHEHFS 280

Query: 250 QNQNNTSESQQDSGHSQGFDRHGSHGRF---PPQNFDAQGAPHQDSSQ--FLEKQQGILS 309
           Q  ++   S  D   +    R  +   F     Q+ +    P QD S     E  Q    
Sbjct: 281 QQHDDIRPSPADISLAPPPPRSVNQDAFRISTRQHSNLITVPIQDDSNSGARETPQAPGP 340

Query: 310 DTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYPPF--PIEGAQQYYNTPYEVSRQD 369
              + +YP    +  P++++P   +    PP P   PP   P++   Q   TP+ V  Q 
Sbjct: 341 TLHHPEYPGQPVVAHPHHIMPPQQHYAPPPPPP---PPISHPMQHPPQAAGTPHMVYSQ- 400

Query: 370 TAAETGSEQGSLLGFPPGAPGGMNFSATYPQSWNTGQAGIPYEHAG---GGQGDYRRSPG 429
                G         PP  P   +  A  P   N    G P +H G        +  +P 
Sbjct: 401 -----GPPPPMTTAPPPITPPPGHIIAQIPPYMNHPPPGPPPQHGGPPVNAPPPHHYNPS 460

Query: 430 RMP-VNSSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYM 435
            MP  N   G         G ++P      P P  PPP M
Sbjct: 461 SMPQFNEDQGTLSPPFTQPGGMSPG---MWPAPRGPPPRM 471

BLAST of IVF0022385 vs. ExPASy Swiss-Prot
Match: Q75N03 (E3 ubiquitin-protein ligase Hakai OS=Homo sapiens OX=9606 GN=CBLL1 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-05
Identity = 100/423 (23.64%), Postives = 145/423 (34.28%), Query Frame = 0

Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCDHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI IYGR+ PC H FC DCA     + D +C  C D +Q+I+ 
Sbjct: 98  LGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQ 157

Query: 126 IKLMEGIFICA-APHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTA 185
                 +F+C+    C +++L + + ++HI+  H    KP       N     +   +  
Sbjct: 158 C-TRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEI 217

Query: 186 SESTVRGPLRPLIS------------PGSNSQPQERDEKFHR---------QQSRDQPRS 245
            E  +  P +  +S            P     P E   + H            +   PRS
Sbjct: 218 PERFIMPPDKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQPHEDIRAPPAELSMAPPPPRS 277

Query: 246 GMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEK 305
             Q+    S  ++ N  +   QD  +S      G+    PP    A   P       +  
Sbjct: 278 VSQETFRISTRKHSNLITVPIQDDSNS------GAREPPPPAPAPAHHHPEYQGQPVVSH 337

Query: 306 QQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEV 365
              I+   P   Y P  P PPP       S+PM  PP   G P      A     T    
Sbjct: 338 PHHIM--PPQQHYAPPPPPPPP------ISHPMPHPPQAAGTPHLVYSQAPPPPMTSAPP 397

Query: 366 SRQDTAAETGSEQGSLLGFPPGAP-----GGMNFSATYPQSWN----------TGQAGIP 425
                     ++    +  PP  P     GG   +A  P  +N           G    P
Sbjct: 398 PITPPPGHIIAQMPPYMNHPPPGPPPPQHGGPPVTAPPPHHYNPNSLPQFTEDQGTLSPP 457

Query: 426 YEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKR 443
           +   GG       SPG  P                   P P +Q PP   P P   H  +
Sbjct: 458 FTQPGG------MSPGIWPAPRGP-------------PPPPRLQGPPSQTPLPGPHHPDQ 486

BLAST of IVF0022385 vs. ExPASy Swiss-Prot
Match: Q4R7I8 (E3 ubiquitin-protein ligase Hakai OS=Macaca fascicularis OX=9541 GN=CBLL1 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-05
Identity = 100/423 (23.64%), Postives = 145/423 (34.28%), Query Frame = 0

Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCDHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI IYGR+ PC H FC DCA     + D +C  C D +Q+I+ 
Sbjct: 97  LGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQ 156

Query: 126 IKLMEGIFICA-APHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTA 185
                 +F+C+    C +++L + + ++HI+  H    KP       N     +   +  
Sbjct: 157 C-TRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEI 216

Query: 186 SESTVRGPLRPLIS------------PGSNSQPQERDEKFHR---------QQSRDQPRS 245
            E  +  P +  +S            P     P E   + H            +   PRS
Sbjct: 217 PERFIMPPDKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQPHEDIRAPPAELSMAPPPPRS 276

Query: 246 GMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEK 305
             Q+    S  ++ N  +   QD  +S      G+    PP    A   P       +  
Sbjct: 277 VSQETFRISTRKHSNLITVPIQDDSNS------GAREPPPPAPAPAHHHPEYQGQPVVSH 336

Query: 306 QQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEV 365
              I+   P   Y P  P PPP       S+PM  PP   G P      A     T    
Sbjct: 337 PHHIM--PPQQHYAPPPPPPPP------ISHPMPHPPQAAGTPHLVYSQAPPPPMTSAPP 396

Query: 366 SRQDTAAETGSEQGSLLGFPPGAP-----GGMNFSATYPQSWN----------TGQAGIP 425
                     ++    +  PP  P     GG   +A  P  +N           G    P
Sbjct: 397 PITPPPGHIIAQMPPYMNHPPPGPPPPQHGGPPVTAPPPHHYNPNSLPQFTEDQGTLSPP 456

Query: 426 YEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKR 443
           +   GG       SPG  P                   P P +Q PP   P P   H  +
Sbjct: 457 FTQPGG------MSPGIWPAPRGP-------------PPPPRLQGPPSQTPLPGPHHPDQ 485

BLAST of IVF0022385 vs. ExPASy Swiss-Prot
Match: Q5ZHZ4 (E3 ubiquitin-protein ligase Hakai OS=Gallus gallus OX=9031 GN=CBLL1 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 4.8e-05
Identity = 96/404 (23.76%), Postives = 152/404 (37.62%), Query Frame = 0

Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCDHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI +YGR+ PC H FC DCA     + D +C  C++ +Q+I+ 
Sbjct: 98  LGEKDDTPVHFCDKCGLPIKMYGRMIPCKHVFCYDCAILHEKKGDKMCPGCNEPVQRIEQ 157

Query: 126 IKLMEGIFICA-APHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTA 185
             +   +F+C+    C +++L + + ++HI+  H    KP                    
Sbjct: 158 C-VRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKP-------------------- 217

Query: 186 SESTVRGPLRPLISPGSNSQPQE---RDEKFHR-----QQSRDQPRSGMQQKQTPSFGQN 245
                  P+ P I+P     P+      EK H      +Q    P   +Q      + Q 
Sbjct: 218 VTRPPLEPVHPPIAPPPAEIPERFIMPPEKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQP 277

Query: 246 QNNTSESQQDSGHSQGFDRHGSHGRF---PPQNFDAQGAPHQDSSQFLEKQQGILSDTPY 305
             +      +   +    R  S   F     ++ +    P QD S    ++    +  P 
Sbjct: 278 HEDIRPPPAEMSMAPPPPRPVSQDTFRISTRKHSNLITVPIQDDSNSGAREPPPPAPAPA 337

Query: 306 SQYPPLQPIP---PPNYVVPANSNPMLTPPLPFGYPPF--PIEGAQQYYNTPYEVSRQDT 365
             +P  Q  P    P++++P   +    PP P   PP   P++   Q   TP+ V  Q  
Sbjct: 338 HHHPEYQGQPVVTHPHHIMPPQQHYAPPPPPP---PPISHPLQHPPQAAGTPHMVYSQAP 397

Query: 366 AAETGSEQGSLLGFPPGAPGGMNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPV 425
                S        PP  P   +  A  P   N    G P    GG   +    P   P 
Sbjct: 398 PPPMTSAP------PPITPPPGHIIAQMPPYMNHPPPGPPPPQHGGPPVNVSAPP---PH 457

Query: 426 NSSAGNAMDIRDGKGILAP---QP------LIQLPPPPPPPPYM 435
           + +  +     + +G L+P   QP      +   P  PPPPP M
Sbjct: 458 HYNPNSLPQFSEDQGTLSPPFTQPGGMSPGIWPAPRGPPPPPRM 468

BLAST of IVF0022385 vs. ExPASy TrEMBL
Match: A0A1S3B5R9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486330 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.3e-271
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360

Query: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 472
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

BLAST of IVF0022385 vs. ExPASy TrEMBL
Match: A0A5A7TKW6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001120 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.3e-271
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360

Query: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 472
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

BLAST of IVF0022385 vs. ExPASy TrEMBL
Match: A0A0A0LDI2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G732590 PE=4 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 3.6e-266
Identity = 462/471 (98.09%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AESGGGAVKPLPAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA GG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 472
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

BLAST of IVF0022385 vs. ExPASy TrEMBL
Match: A0A6J1J5I8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 PE=4 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.5e-240
Identity = 429/506 (84.78%), Postives = 449/506 (88.74%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAE G GAVKP PAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRT+FESHIHESHADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           R PLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQ NTSESQQDSGH
Sbjct: 181 RCPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQINTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDT-AAETGSEQGSLLGFPPGAPG 360
           P NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDT AAETGSEQGSLLGFPPGA G
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTGQAGIPYEHAGGGQ--------------------GDYRRSPGRMPV 420
           GMNFSATYP  WN GQAGIPY+HA GGQ                    GDYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NS--------------SAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 472
           NS              ++G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYPG 480

BLAST of IVF0022385 vs. ExPASy TrEMBL
Match: A0A6J1F6T3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 427/506 (84.39%), Postives = 449/506 (88.74%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAE G GAVKP PAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRT+FESHIHESHADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNN SESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDT-AAETGSEQGSLLGFPPGAPG 360
           P NSNPMLTPPLPFGYPPFP+EGAQ+YY++PYEV RQDT AAETGSEQGSLLGFPPGA G
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQKYYSSPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTGQAGIPYEHAGGGQ--------------------GDYRRSPGRMPV 420
           GMNFSATYP  WN GQAGIPY+HA GGQ                    GDYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NS--------------SAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 472
           NS              ++G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYPG 480

BLAST of IVF0022385 vs. NCBI nr
Match: XP_008442473.1 (PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >XP_008442475.1 PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >KAA0044143.1 E3 ubiquitin-protein ligase Hakai [Cucumis melo var. makuwa] >TYK24994.1 E3 ubiquitin-protein ligase Hakai [Cucumis melo var. makuwa])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360

Query: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

BLAST of IVF0022385 vs. NCBI nr
Match: XP_004137915.1 (E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >XP_031738067.1 E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >KGN58802.1 hypothetical protein Csa_000851 [Cucumis sativus])

HSP 1 Score: 922 bits (2384), Expect = 0.0
Identity = 462/471 (98.09%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AESGGGAVKPLPAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA GG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

BLAST of IVF0022385 vs. NCBI nr
Match: XP_038905987.1 (E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida] >XP_038905988.1 E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida])

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 453/491 (92.26%), Postives = 461/491 (93.89%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAE GGGAVKPLPAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGN+IEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNDIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPL+SPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQTP+FG NQNNTSESQQDSGH
Sbjct: 181 RGPLRPLVSPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQTPNFGPNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGIL+DTPYSQYPPLQ IPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFSEKQQGILADTPYSQYPPLQSIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360
           PANSNP+LTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA GG
Sbjct: 301 PANSNPILTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQG--------------------DYRRSPGRMPVN 420
           MNFSATYPQ WNTGQ GIPYEHAGGGQG                    DYRRSPGRMPVN
Sbjct: 361 MNFSATYPQPWNTGQGGIPYEHAGGGQGMPDAFANTPDSQGKHGFYQGDYRRSPGRMPVN 420

Query: 421 SSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDS 471
           SS GNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKR KFYSGDMDHDGQSLGWQNDS
Sbjct: 421 SSVGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRVKFYSGDMDHDGQSLGWQNDS 480

BLAST of IVF0022385 vs. NCBI nr
Match: XP_023527487.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845 bits (2183), Expect = 5.72e-307
Identity = 431/506 (85.18%), Postives = 451/506 (89.13%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAE G GAVKP PAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRT+FESHIHESHADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQ+S QF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESGQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAPG 360
           P NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGA G
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTGQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 420
           GMNFSATYP  WN GQAGIPY+HA GGQG                    DYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 471
           NS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYPG 480

BLAST of IVF0022385 vs. NCBI nr
Match: KAG6580495.1 (E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017247.1 E3 ubiquitin-protein ligase Hakai [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 839 bits (2168), Expect = 1.10e-304
Identity = 429/506 (84.78%), Postives = 449/506 (88.74%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAE G GAVKP PAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRT+FESHIHESHADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNN SESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSGH 240

Query: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNY V
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYAV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAPG 360
           P NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGA G
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTGQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 420
           GMNFSATYP  WN GQAGIPY+HA GGQG                    DYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHATGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 471
           NS+              +G+ MD+RDGKGILAPQPLIQLPPPP PP ++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPAFLSHNKRGKFYPG 480

BLAST of IVF0022385 vs. TAIR 10
Match: AT5G01160.1 (RING/U-box superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 6.7e-87
Identity = 194/384 (50.52%), Postives = 243/384 (63.28%), Query Frame = 0

Query: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRL +    E+G GA +P P E+VTVACPDHLVLADLPVAKGIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNGA-RPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRL PCDHAFCL+CARSDS+CYLCD+RIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYLCDERIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSA-KQSTASEST 180
           EGIFICAAPHCL+SFLK+ +FE+H+H+ H  LL+ +A+KEDGN+ +  S  +QS+ASEST
Sbjct: 121 EGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASEST 180

Query: 181 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 240
           +R PLR  +                 QQSR+  RS    K    F Q QN   +S  D+ 
Sbjct: 181 LRAPLRSQL-----------------QQSRELNRSASFAKSQSGFSQVQNYPPDS--DNS 240

Query: 241 HSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYP-PLQPIPPPNY 300
              GF+                             + GI     +  YP P+  + PP+ 
Sbjct: 241 RPPGFET-------------------------ASPKPGI----RFPDYPQPMNLMQPPSL 300

Query: 301 VVPANSNPMLTPPLPFGYPPFPIE---GAQQYYN-TPYEVSRQDTAAETGSEQGSLLGFP 360
            VP N NP L  P  FG+P +P      +QQ++N   YE++R ++    GSEQ SLLG+P
Sbjct: 301 PVPMNQNPGL--PQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYP 330

Query: 361 PGAP-GGMNFSATY-PQSWNTGQA 377
           P +P   +NF  +Y P SWN G A
Sbjct: 361 PPSPMTNLNFQGSYPPPSWNPGMA 330